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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 33 |
| AcuI | CTGAAG | 1 cut(s) | 30 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 89 |
| AgsI | TTSAA | 1 cut(s) | 89 |
| ApoI | RAATTY | 1 cut(s) | 33 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 51, 83 |
| BbsI | GAAGAC | 2 cut(s) | 36, 69 |
| BccI | CCATC | 1 cut(s) | 136 |
| BfaI | CTAG | 1 cut(s) | 17 |
| BmiI | GGNNCC | 1 cut(s) | 94 |
| BmrI | ACTGGG | 1 cut(s) | 33 |
| BmuI | ACTGGG | 1 cut(s) | 33 |
| BpiI | GAAGAC | 2 cut(s) | 36, 69 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 89 |
| Bse1I | ACTGG | 1 cut(s) | 28 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 89 |
| BseNI | ACTGG | 1 cut(s) | 28 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 89 |
| BspLI | GGNNCC | 1 cut(s) | 94 |
| BsrI | ACTGG | 1 cut(s) | 28 |
| BstV2I | GAAGAC | 2 cut(s) | 36, 69 |
| BtsI | GCAGTG | 1 cut(s) | 169 |
| BtsIMutI | CAGTG | 1 cut(s) | 169 |
| CviAII | CATG | 1 cut(s) | 168 |
| CviJI | RGCY | 2 cut(s) | 13, 93 |
| CviKI_1 | RGCY | 2 cut(s) | 13, 93 |
| Eco57I | CTGAAG | 1 cut(s) | 30 |
| FaeI | CATG | 1 cut(s) | 171 |
| FaiI | YATR | 3 cut(s) | 41, 56, 169 |
| FatI | CATG | 1 cut(s) | 167 |
| FspBI | CTAG | 1 cut(s) | 17 |
| Hin1II | CATG | 1 cut(s) | 171 |
| HinfI | GANTC | 1 cut(s) | 124 |
| Hpy188I | TCNGA | 1 cut(s) | 49 |
| Hpy188III | TCNNGA | 2 cut(s) | 17, 132 |
| HpyAV | CCTTC | 1 cut(s) | 83 |
| HpyCH4V | TGCA | 2 cut(s) | 31, 167 |
| Hsp92II | CATG | 1 cut(s) | 171 |
| LmnI | GCTCC | 1 cut(s) | 98 |
| LpnPI | CCDG | 1 cut(s) | 9 |
| MaeI | CTAG | 1 cut(s) | 17 |
| MboII | GAAGA | 4 cut(s) | 17, 36, 71, 74 |
| MluCI | AATT | 1 cut(s) | 33 |
| MnlI | CCTC | 2 cut(s) | 24, 145 |
| NlaIII | CATG | 1 cut(s) | 171 |
| NlaIV | GGNNCC | 1 cut(s) | 94 |
| PfeI | GAWTC | 1 cut(s) | 124 |
| PspN4I | GGNNCC | 1 cut(s) | 94 |
| SgeI | CNNG | 5 cut(s) | 29, 36, 94, 144, 157 |
| Sse9I | AATT | 1 cut(s) | 33 |
| SspMI | CTAG | 1 cut(s) | 17 |
| TasI | AATT | 1 cut(s) | 33 |
| TfiI | GAWTC | 1 cut(s) | 124 |
| TscAI | CASTG | 1 cut(s) | 169 |
| TspDTI | ATGAA | 1 cut(s) | 132 |
| TspRI | CASTG | 1 cut(s) | 169 |
| XapI | RAATTY | 1 cut(s) | 33 |
| XbaI | TCTAGA | 1 cut(s) | 16 |
| XspI | CTAG | 1 cut(s) | 17 |