RchiOBHm_Chr1g0329481

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000143
Physical Location & Seq
Forward (+)
19136487 .. 19145579
9093 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55879

Sequence Viewer

Length: 3510 bp
ATGGATCCCAACGAGACCGCACAGCAGCTTCTTGCCCTTCATGCTAGAATTGTGGGAAACATGGAGTCAGAGACTATGGTCTCTACCACAGATCAACGTGTTGAAGAAGAGCCACAGTACACACATATTGACCAGAATGAATTCTCACAGAGAAGGCACAAGAAGCAGGAGGAGGAGGAGATTGTCATATGTGAATGCAAGTATGATGAAAAGGATCCTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGTGTTGACCAGCACGGAATGCACTCCTGGATATTGCCCTTGTGGTGTCTATTGTAAGAATCAGAGATTTCAGAAATGTGAATATGCCAAGACAAAATTGTTTAAAACAGAAGGCCGTGGTTGGGGTCTTTTAGCCAATGAGAACATTAAGGCAGGGCAGTTTATTATTGAATACTGTGGAGAAGTGATATCATGGAAGGAAGCAAAGAAAAGATCTCATGCTTATGAAGTTGAAGGTATCAGGGATGCATTTATTATTTCTCTCAATGCCTCTGAGTCTATTGATGCCACTGTGAAGGGAAGCCTAGCAAGATTTATAAATCATTCTTGCCAACCAAATTGTGAGACAAGAAAGTGGAATGTGCTGGGGGAAATTAGGGTTGGAATATTTGCAAAACAAGATATATTAGCTGGAACTGAGTTGGCATATGACTACAATTTTGAATGGTATGGGGGTGTGAAGGTTCGCTGCCTCTGTGGTGCACCCAGCTGTTCGGGATTTCTTGGGGCAAAATCTCGTGGTTTTCAGCATCAAATCATCATCTATGACGGCTCTAAGGATGAAAAGGGTGATACTTCTAGTCGAGGTAGTGGGGTTGGGTCTGATACTGCAATGGATTCCACGACTCAATCTAGTACTCATGAGGCCCACACTTATACTCGAGGTAGTGAGGTCGACTCAGAATGTGTAATGGATTTCACAAGTGAATTTACTACTTATGAGATTTTCAAGAGTAGAGAATCCTTGATGCTGTGGGCTCGTGAGGCCGGGAAACGAAACGGGTTTGTTGTTGTGACGGTACGATCTGATTTGGGTGGTAAAACTTTGAAACCAAGAGTTACATTAGGGTGTGAGCGTGGAGGAAAATTTAAGGTTCACAAAGAAAGATGTGATAAAGGTGGGAAGCAAAGATGTGGGACTGGCAGCAAGAAGTGTGGCTGTCCTTTCACATTAAAGGGAGAGAAGTTAACAACTGGTGATGGATGGATGCTAAAGGTAGTATGTGGTGTGCACAATCATGCCTTAACCGAGTCTCTTGAAGGGCACTCATATGCAGGGAGGTTGTCAGAGGAGGAGACTTCACTCTTGATAGACTTATCAAAGAGTTTAGTACGACCCAAAGAAATCTTGAACAAATTGAAGCAAAGGGATTCACTCAATGTTACAACTATAAGAACTATCTACAATGCACGCCAGAGGCAAAGAGTTAAAGAGAAAGCAGGGAGGTCTCAAATGGAACAACTTTTATGTAAGTTGAATGAGCATAATTATCTTGAGTGGCACAGGAGTTGCCCTATTTCAGGGAGTGTGCTGGACTTGTTTTGGGCACACCCTGTGAGCTTGGAATTGTTACGTGTATTTCCAACCATCTTGATGATGGATTGCACTTTTAAAACCAATAGGTATCGTTATCCTCTCCTAGAGGTTGTGGGTGTGACATCGACTAATATGACATTCTCAATTGCATTTGTATTTTTGGATTCTGAAAAGGAAGATAATTATGTGTGGGCCTTGAATAGATTACGTGATGTGTTGGATGGGTGTGCTATGCCCAATGTTATTGTCACAGACAAGGATTTGGAATTGATGAGAGCCGTTGAGATGGTATTTCCTTCAGCTAGACATATGCTGTGCAAATGGCATATCAATAAGAATGTGTTGGCAAAATGCAGAAATCTGTTTGAGTCCAAGGAAAAGTGGGATATGTTTATTATGAAGTGGAATGTGCTTGTGATGTCACCTACTGAAGAAGACTACATACAAGAGCTAGCAGTGCTACAGAGTGAATTTAGGAATTACACAGACGTCTTGGACTATGTTACAAACACTTGGTTGAATCCTTTTAGGGACCGATTTGTGTCGGCCTGGACAGACGTAATCATGCATTTTGGTAATGTGGCATCAAACAGGGCGGAGAGTGCACATTCTAAGCTTAAGAGGCAACTTGGTTTGAGTCAGGGGAATTTTGAGGGTTCTTTTGAGAAGATACACGCTCTGCTTGAGTTACAACACACTGACATTAAGGCATCTTTCGAGAAATGTTTGACTGTTGTACGACATAGCTCCTTGCCTTTAGAATTCAAACATCTAAGAGGAGTAGTTTCTACTTTTGCATTGGACAAGGTATTATCTCAGTTTAAGCTAGCCACCTCAATTGGAATTGATGATTTTCATTGTGGTTGTGTCATACGTCGCACCCATGGTTTACCTTGTGCCCATGAAATTGCCAAATATAAGAGGGATGGTCAACCAATTCCGCTCGATTCTCTTGACCCCCATTGGAGAAAACTTCATATGTCACCCTCTTCTGATCCTGTTAATGATAATATGGATGTTAATGAATTTCCTGAGTTGGAGTTGCTTCAACAGTACTTTTTAAAGACAAATGCTGATAATCGGTTGATGCTGCGGAAAAGAGTGAGAGAACTTGTACCATCCCTAGCAACTCTTATTGAACCAGGGGTGAAGTCCAGAACACGAGGTCAATTGAAGGAGAGAATTGATATGCCCATTCAGAAAAACGCATCTGTGTTTGAGTTAATACAATCTGCATCTGTGGATAGTCATTCACCTAGTGTGACTGGTGGTACAATGGATGTTTTGAAAAAATCAACACATAAGCAGACAGGGCCGATGAAGGAGAAGGTTTACCGGACACTTTCAGCAAAAGTTATGTCATATATTGATTCATTTCCAGTGGGATTGATGCCTTACATAGACCAAGTCAAGGATGTATGTGATGATGGTAACTGTGGGTTTAGAGCGGTCGCTGATTTAGTAGGATTGGGCGAGAACGCATACTTGCAAGTGAGAAATGACCTACTGACTGAATTGTCTTCCTATTCTGCACATTATGGTGAGCTATACGGCAGCGCAGAGAAGGTTCGTGACTTGGCACAATCACTTTCATTCTTCAATGAGGGTACAGCGCCATTTGACAGATGCATGACAATGCCTGACATGGGTCATCTCATAGCATCATGCTATAAGATTGTACTCTTCCACTTTTCAGCTACTCAATGCCTTACCTTCCTACCTCTTCGATCAACACCTGTTCCTTTGCCAATGCGTAGACATATTGCTATTGGTCTTGTTAATGATTCCCATTATGTTGAGGTCTTCTTAAAGCCAGGACATCCTGTACCACCCATTGAGTCTAATTGGATCAGATATCATACACAAGCTGCAAAAGGGTGGGATACTATATATACTGATTGTATTGCACACTTCCAGTTGCTTGCCCGCTCGGATAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
FAR1 MULE SET

Protein Analysis

1169

Amino Acids

132.51

Weight (kDa)

7.2

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWS PF17907 72 - 109 3.9e-14 AWS domain
SET PF00856 123 - 229 5.7e-25 SET domain
ZSWIM1-3_RNaseH-like PF21056 532 - 625 2.7e-11 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 541 - 635 2.7e-26 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76710 AT1G76710 AT1G76710 AT1G76710
fragaria_vesca FvH4_1g20063 FvH4_2g15231 FvH4_3g04011 FvH4_3g28681 FvH4_4g01411 FvH4_4g10181 FvH4_4g15922 FvH4_4g16651 FvH4_4g16652 FvH4_5g13892 FvH4_5g13893 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g25511 FvH4_6g12551 FvH4_6g12552 FvH4_6g38841 FvH4_7g04391
malus_domestica MD05G1031300.v1.1 MD05G1031400.v1.1 MD05G1031500.v1.1 MD08G1164400.v1.1 MD10G1032900.v1.1 MD10G1033000.v1.1 MD10G1033100.v1.1
prunus_persica Prupe.3G240700_v2.0.a1 Prupe.7G144600_v2.0.a1 Prupe.8G038500_v2.0.a1 Prupe.8G038500_v2.0.a1
pyrus_communis pycom05g02250 pycom05g02260 pycom05g02270 pycom05g02280 pycom08g14160 pycom10g02100 pycom10g02110 pycom10g02130
rosa_chinensis RchiOBHm_Chr1g0329481 RchiOBHm_Chr1g0329491 RchiOBHm_Chr1g0341531 RchiOBHm_Chr3g0464811 RchiOBHm_Chr3g0494701 RchiOBHm_Chr3g0497501 RchiOBHm_Chr4g0385841 RchiOBHm_Chr5g0061181 RchiOBHm_Chr6g0263001 RchiOBHm_Chr7g0193111 RchiOBHm_Chr7g0206281 RchiOBHm_Chr7g0206311
rosa_laevigata RLG00000018908 RLG00000028528 RLG00000028803 RLG00000029882 RLG00000029883 RLG00000030361
rosa_multiflora Rmu_co8196328.1_g000001 Rmu_co8259135.1_g000001 Rmu_co8269185.1_g000001 Rmu_sc0000076.1_g000019 Rmu_sc0000311.1_g000007 Rmu_sc0000429.1_g000038 Rmu_sc0000429.1_g000039 Rmu_sc0000429.1_g000040 Rmu_sc0000511.1_g000005 Rmu_sc0000539.1_g000054 Rmu_sc0000566.1_g000039 Rmu_sc0000805.1_g000032 Rmu_sc0000870.1_g000073 Rmu_sc0000870.1_g000074 Rmu_sc0001010.1_g000018 Rmu_sc0001083.1_g000013 Rmu_sc0001114.1_g000011 Rmu_sc0001702.1_g000027 Rmu_sc0001779.1_g000012 Rmu_sc0002923.1_g000036 Rmu_sc0003242.1_g000011 Rmu_sc0003291.1_g000036 Rmu_sc0003393.1_g000012 Rmu_sc0003872.1_g000016 Rmu_sc0003872.1_g000017 Rmu_sc0003872.1_g000018 Rmu_sc0004084.1_g000020 Rmu_sc0004386.1_g000005 Rmu_sc0004622.1_g000005 Rmu_sc0004742.1_g000021 Rmu_sc0004755.1_g000020 Rmu_sc0004805.1_g000036 Rmu_sc0005065.1_g000006 Rmu_sc0005177.1_g000004 Rmu_sc0006373.1_g000003 Rmu_sc0006373.1_g000004 Rmu_sc0007121.1_g000002 Rmu_sc0007943.1_g000003 Rmu_sc0010368.1_g000006 Rmu_sc0010543.1_g000001 Rmu_sc0012591.1_g000006 Rmu_sc0013504.1_g000009 Rmu_sc0015025.1_g000001 Rmu_sc0016170.1_g000001 Rmu_sc0017156.1_g000001 Rmu_sc0026439.1_g000001
rosa_roxburghii Rroxscaffold_175G00432280 Rroxscaffold_1G00030190 Rroxscaffold_1G00030880 Rroxscaffold_2G00090520 Rroxscaffold_3G00227940 Rroxscaffold_3G00233400 Rroxscaffold_3G00238500 Rroxscaffold_3G00251650 Rroxscaffold_4G00301570 Rroxscaffold_4G00321040 Rroxscaffold_5G00350880 Rroxscaffold_7G00184400
rosa_rugosa Rorug01G0080800 Rorug01G0080900 Rorug01G0193900 Rorug02G0275900 Rorug02G0275900 Rorug02G0275900 Rorug02G0305400 Rorug02G0311800 Rorug02G0350400 Rorug02G0381300 Rorug03G0136700 Rorug03G0181900 Rorug03G0268000 Rorug04G0301600 Rorug05G0175100 Rorug05G0250700 Rorug05G0298200 Rorug05G0596000 Rorug06G0029500 Rorug07G0094300 Rorug07G0094300 Rorug07G0095400 Rorug07G0140100
rosa_samantha Rh1AG099200 Rh1AG190500 Rh1BG078900 Rh1BG079000 Rh1BG079100 Rh1CG084600 Rh1CG084700 Rh1CG095300 Rh1CG175900 Rh1DG103300 Rh1DG150400 Rh2AG079300 Rh2AG272400 Rh2AG464800 Rh2AG507400 Rh2DG078300 Rh2DG078400 Rh3CG206900 Rh3CG295400 Rh3DG206800 Rh3DG356200 Rh4CG071500 Rh4CG179200 Rh4DG064700 Rh5CG362400 Rh7AG212300 Rh7AG226200 Rh7BG222200
rosa_wichuraiana Rw0G009990 Rw0G011260 Rw0G011270 Rw0G012910 Rw0G013740 Rw1G007740 Rw1G013050 Rw1G014230 Rw2G014270 Rw3G012720 Rw3G028110 Rw4G006630 Rw4G015270 Rw4G016160 Rw4G031380 Rw5G011440 Rw5G029180 Rw5G048360 Rw5G048430 Rw5G048780 Rw6G005250 Rw6G006390 Rw7G002840 Rw7G014080 Rw7G019650 Rw7G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AasI GACNNNNNNGTC 1 cut(s) 3082
AatII GACGTC 1 cut(s) 2058
AccBSI CCGCTC 3 cut(s) 2509, 3014, 3495
AccI GTMKAC 2 cut(s) 924, 3322
AciI CCGC 6 cut(s) 18, 2162, 2507, 2659, 3014, 3493
AclWI GGATC 5 cut(s) 12, 209, 222, 2555, 3422
AcsI RAATTY 7 cut(s) 140, 956, 1115, 2036, 2212, 2327, 2591
AcuI CTGAAG 2 cut(s) 1848, 2016
AcyI GRCGYC 1 cut(s) 2055
AdeI CACNNNGTG 2 cut(s) 1585, 2825
AfiI CCNNNNNNNGG 4 cut(s) 370, 1550, 2977, 3212
AflII CTTAAG 1 cut(s) 2183
AflIII ACRYGT 2 cut(s) 97, 1603
AjnI CCWGG 4 cut(s) 274, 2114, 2707, 3379
AjuI GAANNNNNNNTTGG 2 cut(s) 612, 644
Alw21I GWGCWC 3 cut(s) 733, 1263, 2173
Alw26I GTCTC 7 cut(s) 8, 65, 85, 587, 1287, 1319, 1482
Alw44I GTGCAC 3 cut(s) 729, 1259, 2169
AlwI GGATC 5 cut(s) 12, 209, 222, 2555, 3422
Ama87I CYCGRG 1 cut(s) 909
AoxI GGCC 6 cut(s) 361, 894, 1014, 1758, 2112, 2879
ApaLI GTGCAC 3 cut(s) 729, 1259, 2169
ApeKI GCWGC 6 cut(s) 25, 717, 1173, 2656, 3120, 3434
ApoI RAATTY 7 cut(s) 140, 956, 1115, 2036, 2212, 2327, 2591
ArsI GACNNNNNNTTYG 4 cut(s) 671, 703, 2909, 2941
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 2 cut(s) 3125, 3181
AspS9I GGNCC 4 cut(s) 895, 1758, 2098, 2879
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 7 cut(s) 830, 1238, 1980, 2541, 2725, 2811, 3119
AsuNHI GCTAGC 2 cut(s) 2017, 2392
AvaI CYCGRG 1 cut(s) 909
AvaII GGWCC 1 cut(s) 2098
BaeGI GKGCMC 6 cut(s) 733, 1263, 1296, 1579, 2173, 2467
BamHI GGATCC 2 cut(s) 4, 214
BanII GRGCYC 1 cut(s) 1009
BarI GAAGNNNNNNTAC 2 cut(s) 1992, 2024
BauI CACGAG 3 cut(s) 765, 1008, 2727
BbsI GAAGAC 3 cut(s) 2007, 3078, 3361
Bbv12I GWGCWC 3 cut(s) 733, 1263, 2173
BbvI GCAGC 6 cut(s) 37, 704, 1185, 2643, 3132, 3421
BccI CCATC 9 cut(s) 1223, 1227, 1621, 1625, 1781, 1846, 2486, 2692, 2987
BceAI ACGGC 4 cut(s) 348, 814, 1829, 3133
BciT130I CCWGG 4 cut(s) 276, 2116, 2709, 3381
BciVI GTATCC 1 cut(s) 3442
BcnI CCSGG 1 cut(s) 1018
BcoDI GTCTC 7 cut(s) 8, 65, 85, 587, 1287, 1319, 1482
BfmI CTRYAG 1 cut(s) 2027
BfoI RGCGCY 1 cut(s) 3182
BfrI CTTAAG 1 cut(s) 2183
BfuI GTATCC 1 cut(s) 3442
BglII AGATCT 1 cut(s) 461
BisI GCNGC 6 cut(s) 26, 718, 1174, 2657, 3121, 3435
BlsI GCNGC 6 cut(s) 27, 719, 1175, 2658, 3122, 3436
BmcAI AGTACT 2 cut(s) 886, 2621
Bme1390I CCNGG 5 cut(s) 276, 1018, 2116, 2709, 3381
Bme18I GGWCC 1 cut(s) 2098
BmeT110I CYCGRG 1 cut(s) 909
BmgT120I GGNCC 4 cut(s) 895, 1758, 2098, 2879
BmiI GGNNCC 3 cut(s) 6, 216, 2099
BmrFI CCNGG 5 cut(s) 276, 1018, 2116, 2709, 3381
BmtI GCTAGC 2 cut(s) 2021, 2396
BoxI GACNNNNGTC 2 cut(s) 77, 3213
BpiI GAAGAC 3 cut(s) 2007, 3078, 3361
BplI GAGNNNNNCTC 2 cut(s) 911, 943
BpuEI CTTGAG 2 cut(s) 1544, 2270
BpuMI CCSGG 1 cut(s) 1018
BsaAI YACGTR 2 cut(s) 1604, 1775
BsaHI GRCGYC 1 cut(s) 2055
BsaI GGTCTC 3 cut(s) 8, 85, 1482
BsaJI CCNNGG 4 cut(s) 364, 1938, 2449, 2708
BsaWI WCCGGW 1 cut(s) 2901
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 1550, 2977, 3212
Bse1I ACTGG 5 cut(s) 1174, 1228, 2836, 2945, 3481
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 4 cut(s) 276, 2116, 2709, 3381
BseDI CCNNGG 4 cut(s) 364, 1938, 2449, 2708
BseLI CCNNNNNNNGG 4 cut(s) 370, 1550, 2977, 3212
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 5 cut(s) 513, 657, 942, 2396, 2589
BseNI ACTGG 5 cut(s) 1174, 1228, 2836, 2945, 3481
BseRI GAGGAG 6 cut(s) 185, 188, 191, 1334, 1337, 2358
BseSI GKGCMC 6 cut(s) 733, 1263, 1296, 1579, 2173, 2467
BseXI GCAGC 6 cut(s) 37, 704, 1185, 2643, 3132, 3421
BseYI CCCAGC 2 cut(s) 613, 734
BsgI GTGCAG 1 cut(s) 3081
Bsh1285I CGRYCG 1 cut(s) 3018
BshFI GGCC 6 cut(s) 363, 896, 1016, 1760, 2114, 2881
BsiEI CGRYCG 1 cut(s) 3018
BsiHKAI GWGCWC 3 cut(s) 733, 1263, 2173
BsiHKCI CYCGRG 1 cut(s) 909
BsiSI CCGG 2 cut(s) 1017, 2902
BslFI GGGAC 2 cut(s) 1180, 2111
BslI CCNNNNNNNGG 4 cut(s) 370, 1550, 2977, 3212
BsmAI GTCTC 7 cut(s) 8, 65, 85, 587, 1287, 1319, 1482
BsmFI GGGAC 2 cut(s) 1180, 2111
BsmI GAATGC 2 cut(s) 200, 272
BsnI GGCC 6 cut(s) 363, 896, 1016, 1760, 2114, 2881
Bso31I GGTCTC 3 cut(s) 8, 85, 1482
BsoBI CYCGRG 1 cut(s) 909
Bsp1286I GDGCHC 7 cut(s) 733, 1009, 1263, 1296, 1579, 2173, 2467
Bsp143I GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
Bsp19I CCATGG 1 cut(s) 2449
BspACI CCGC 6 cut(s) 18, 2162, 2507, 2659, 3014, 3493
BspANI GGCC 6 cut(s) 363, 896, 1016, 1760, 2114, 2881
BspCNI CTCAG 5 cut(s) 514, 658, 941, 2395, 2590
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 3 cut(s) 6, 216, 2099
BspOI GCTAGC 2 cut(s) 2021, 2396
BspPI GGATC 5 cut(s) 12, 209, 222, 2555, 3422
BspQI GCTCTTC 1 cut(s) 102
BspTI CTTAAG 1 cut(s) 2183
BspTNI GGTCTC 3 cut(s) 8, 85, 1482
BsrBI CCGCTC 3 cut(s) 2509, 3014, 3495
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 5 cut(s) 1174, 1228, 2836, 2945, 3481
BssECI CCNNGG 4 cut(s) 364, 1938, 2449, 2708
BssMI GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
BssNI GRCGYC 1 cut(s) 2055
BssSI CACGAG 3 cut(s) 765, 1008, 2727
BssT1I CCWWGG 2 cut(s) 1938, 2449
Bst2BI CACGAG 3 cut(s) 765, 1008, 2727
Bst2UI CCWGG 4 cut(s) 276, 2116, 2709, 3381
Bst4CI ACNGT 7 cut(s) 117, 425, 541, 1048, 2299, 2619, 3002
Bst6I CTCTTC 4 cut(s) 102, 2560, 3254, 3294
BstACI GRCGYC 1 cut(s) 2055
BstAFI CTTAAG 1 cut(s) 2183
BstAPI GCANNNNNTGC 1 cut(s) 267
BstBAI YACGTR 2 cut(s) 1604, 1775
BstC8I GCNNGC 5 cut(s) 1441, 2019, 2394, 3489, 3493
BstDEI CTNAG 8 cut(s) 522, 666, 804, 928, 2178, 2339, 2382, 2598
BstDSI CCRYGG 2 cut(s) 364, 2449
BstENI CCTNNNNNAGG 1 cut(s) 1548
BstH2I RGCGCY 1 cut(s) 3182
BstHHI GCGC 2 cut(s) 3125, 3181
BstKTI GATC 8 cut(s) 7, 94, 217, 464, 1055, 2563, 3296, 3417
BstMAI GTCTC 7 cut(s) 8, 65, 85, 587, 1287, 1319, 1482
BstMBI GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
BstMCI CGRYCG 1 cut(s) 3018
BstMWI GCNNNNNNNGC 8 cut(s) 41, 163, 267, 1013, 2023, 2168, 2188, 2878
BstNI CCWGG 4 cut(s) 276, 2116, 2709, 3381
BstNSI RCATGY 1 cut(s) 231
BstPAI GACNNNNGTC 2 cut(s) 77, 3213
BstSCI CCNGG 5 cut(s) 274, 1016, 2114, 2707, 3379
BstSFI CTRYAG 1 cut(s) 2027
BstSLI GKGCMC 6 cut(s) 733, 1263, 1296, 1579, 2173, 2467
BstV1I GCAGC 6 cut(s) 37, 704, 1185, 2643, 3132, 3421
BstV2I GAAGAC 3 cut(s) 2007, 3078, 3361
BstX2I RGATCY 3 cut(s) 4, 214, 461
BstYI RGATCY 3 cut(s) 4, 214, 461
BsuI GTATCC 1 cut(s) 3442
BsuRI GGCC 6 cut(s) 363, 896, 1016, 1760, 2114, 2881
BtgI CCRYGG 2 cut(s) 364, 2449
BtsI GCAGTG 1 cut(s) 2028
BtsIMutI CAGTG 4 cut(s) 537, 2028, 2262, 2952
Cac8I GCNNGC 5 cut(s) 1441, 2019, 2394, 3489, 3493
CciI TCATGA 1 cut(s) 889
CfoI GCGC 2 cut(s) 3125, 3181
Cfr13I GGNCC 4 cut(s) 895, 1758, 2098, 2879
CspCI CAANNNNNGTGG 4 cut(s) 1165, 1200, 2386, 2421
DdeI CTNAG 8 cut(s) 522, 666, 804, 928, 2178, 2339, 2382, 2598
DpnI GATC 8 cut(s) 6, 93, 216, 463, 1054, 2562, 3295, 3416
DpnII GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
DraI TTTAAA 3 cut(s) 352, 1642, 2628
DraIII CACNNNGTG 2 cut(s) 1585, 2825
DrdI GACNNNNNNGTC 1 cut(s) 3082
DseDI GACNNNNNNGTC 1 cut(s) 3082
Eam1104I CTCTTC 4 cut(s) 102, 2560, 3254, 3294
EarI CTCTTC 4 cut(s) 102, 2560, 3254, 3294
EciI GGCGGA 1 cut(s) 2177
Eco130I CCWWGG 2 cut(s) 1938, 2449
Eco24I GRGCYC 1 cut(s) 1009
Eco31I GGTCTC 3 cut(s) 8, 85, 1482
Eco32I GATATC 2 cut(s) 438, 3422
Eco47I GGWCC 1 cut(s) 2098
Eco57I CTGAAG 2 cut(s) 1848, 2016
Eco88I CYCGRG 1 cut(s) 909
EcoNI CCTNNNNNAGG 1 cut(s) 1548
EcoRI GAATTC 2 cut(s) 140, 2327
EcoRII CCWGG 4 cut(s) 274, 2114, 2707, 3379
EcoRV GATATC 2 cut(s) 438, 3422
EcoT14I CCWWGG 2 cut(s) 1938, 2449
EcoT22I ATGCAT 3 cut(s) 499, 2136, 3197
EcoT38I GRGCYC 1 cut(s) 1009
ErhI CCWWGG 2 cut(s) 1938, 2449
FaqI GGGAC 2 cut(s) 1180, 2111
FauI CCCGC 1 cut(s) 3500
FauNDI CATATG 5 cut(s) 188, 676, 1300, 1875, 2544
FblI GTMKAC 2 cut(s) 924, 3322
Fnu4HI GCNGC 6 cut(s) 26, 718, 1174, 2657, 3121, 3435
FriOI GRGCYC 1 cut(s) 1009
Fsp4HI GCNGC 6 cut(s) 26, 718, 1174, 2657, 3121, 3435
GlaI GCGC 2 cut(s) 3124, 3180
GluI GCNGC 6 cut(s) 26, 718, 1174, 2657, 3121, 3435
GsaI CCCAGC 2 cut(s) 617, 738
HaeII RGCGCY 1 cut(s) 3182
HaeIII GGCC 6 cut(s) 363, 896, 1016, 1760, 2114, 2881
HapII CCGG 2 cut(s) 1017, 2902
HhaI GCGC 2 cut(s) 3125, 3181
Hin1I GRCGYC 1 cut(s) 2055
Hin6I GCGC 2 cut(s) 3123, 3179
HinP1I GCGC 2 cut(s) 3123, 3179
HincII GTYRAC 4 cut(s) 255, 925, 1218, 2498
HindII GTYRAC 4 cut(s) 255, 925, 1218, 2498
HindIII AAGCTT 1 cut(s) 2180
HpaI GTTAAC 1 cut(s) 1218
HpaII CCGG 2 cut(s) 1017, 2902
HphI GGTGA 7 cut(s) 830, 1238, 1980, 2541, 2725, 2811, 3119
Hpy99I CGWCG 1 cut(s) 2445
HpyCH4III ACNGT 7 cut(s) 117, 425, 541, 1048, 2299, 2619, 3002
HpyCH4IV ACGT 6 cut(s) 97, 1603, 1774, 2055, 2124, 2440
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 163, 267, 1013, 2023, 2168, 2188, 2878
HpyF3I CTNAG 8 cut(s) 522, 666, 804, 928, 2178, 2339, 2382, 2598
HpySE526I ACGT 6 cut(s) 97, 1603, 1774, 2055, 2124, 2440
Hsp92I GRCGYC 1 cut(s) 2055
HspAI GCGC 2 cut(s) 3123, 3179
KspAI GTTAAC 1 cut(s) 1218
Kzo9I GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 2318
Lsp1109I GCAGC 6 cut(s) 37, 704, 1185, 2643, 3132, 3421
MaeII ACGT 6 cut(s) 97, 1603, 1774, 2055, 2124, 2440
MalI GATC 8 cut(s) 6, 93, 216, 463, 1054, 2562, 3295, 3416
MbiI CCGCTC 3 cut(s) 2509, 3014, 3495
MboI GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
MfeI CAATTG 3 cut(s) 1710, 2403, 2735
MflI RGATCY 3 cut(s) 4, 214, 461
MhlI GDGCHC 7 cut(s) 733, 1009, 1263, 1296, 1579, 2173, 2467
MlyI GAGTC 8 cut(s) 74, 533, 868, 920, 1289, 1943, 2212, 3413
MmeI TCCRAC 4 cut(s) 610, 1637, 1764, 2583
Mph1103I ATGCAT 3 cut(s) 499, 2136, 3197
MroXI GAANNNNTTC 1 cut(s) 140
MslI CAYNNNNRTG 7 cut(s) 471, 1096, 1266, 1299, 1622, 3105, 3200
MspA1I CMGCKG 1 cut(s) 738
MspCI CTTAAG 1 cut(s) 2183
MspI CCGG 2 cut(s) 1017, 2902
MspR9I CCNGG 5 cut(s) 276, 1018, 2116, 2709, 3381
MunI CAATTG 3 cut(s) 1710, 2403, 2735
Mva1269I GAATGC 2 cut(s) 200, 272
MvaI CCWGG 4 cut(s) 276, 2116, 2709, 3381
MwoI GCNNNNNNNGC 8 cut(s) 41, 163, 267, 1013, 2023, 2168, 2188, 2878
NciI CCSGG 1 cut(s) 1018
NcoI CCATGG 1 cut(s) 2449
NdeI CATATG 5 cut(s) 188, 676, 1300, 1875, 2544
NdeII GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
NheI GCTAGC 2 cut(s) 2017, 2392
NlaIV GGNNCC 3 cut(s) 6, 216, 2099
NmuCI GTSAC 7 cut(s) 1042, 1684, 1813, 1986, 2547, 2827, 3137
NsiI ATGCAT 3 cut(s) 499, 2136, 3197
NspI RCATGY 1 cut(s) 231
PaeR7I CTCGAG 1 cut(s) 909
PagI TCATGA 1 cut(s) 889
PciSI GCTCTTC 1 cut(s) 102
PctI GAATGC 2 cut(s) 200, 272
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 9 cut(s) 307, 866, 989, 1400, 1730, 2086, 2513, 2936, 3350
PflFI GACNNNGTC 1 cut(s) 2972
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 6 cut(s) 27, 719, 1175, 2658, 3122, 3436
PleI GAGTC 8 cut(s) 73, 532, 868, 920, 1288, 1942, 2211, 3412
PpsI GAGTC 8 cut(s) 73, 532, 868, 920, 1288, 1942, 2211, 3412
Ppu21I YACGTR 2 cut(s) 1604, 1775
PshAI GACNNNNGTC 2 cut(s) 77, 3213
PsiI TTATAA 1 cut(s) 566
Psp6I CCWGG 4 cut(s) 274, 2114, 2707, 3379
PspFI CCCAGC 2 cut(s) 613, 734
PspGI CCWGG 4 cut(s) 274, 2114, 2707, 3379
PspN4I GGNNCC 3 cut(s) 6, 216, 2099
PspPI GGNCC 4 cut(s) 895, 1758, 2098, 2879
PspXI VCTCGAGB 1 cut(s) 909
PsuI RGATCY 3 cut(s) 4, 214, 461
PsyI GACNNNGTC 1 cut(s) 2972
PvuII CAGCTG 1 cut(s) 738
RseI CAYNNNNRTG 7 cut(s) 471, 1096, 1266, 1299, 1622, 3105, 3200
SalI GTCGAC 1 cut(s) 923
SapI GCTCTTC 1 cut(s) 102
SatI GCNGC 6 cut(s) 26, 718, 1174, 2657, 3121, 3435
Sau3AI GATC 8 cut(s) 4, 91, 214, 461, 1052, 2560, 3293, 3414
Sau96I GGNCC 4 cut(s) 895, 1758, 2098, 2879
ScaI AGTACT 2 cut(s) 886, 2621
SchI GAGTC 8 cut(s) 74, 533, 868, 920, 1289, 1943, 2212, 3413
ScrFI CCNGG 5 cut(s) 276, 1018, 2116, 2709, 3381
SduI GDGCHC 7 cut(s) 733, 1009, 1263, 1296, 1579, 2173, 2467
SfcI CTRYAG 1 cut(s) 2027
Sfr274I CTCGAG 1 cut(s) 909
SinI GGWCC 1 cut(s) 2098
SlaI CTCGAG 1 cut(s) 909
SmiMI CAYNNNNRTG 7 cut(s) 471, 1096, 1266, 1299, 1622, 3105, 3200
SmlI CTYRAG 4 cut(s) 909, 1523, 2183, 2249
SmoI CTYRAG 4 cut(s) 909, 1523, 2183, 2249
SsiI CCGC 6 cut(s) 18, 2162, 2507, 2659, 3014, 3493
SspI AATATT 1 cut(s) 636
StyD4I CCNGG 5 cut(s) 274, 1016, 2114, 2707, 3379
StyI CCWWGG 2 cut(s) 1938, 2449
TaaI ACNGT 7 cut(s) 117, 425, 541, 1048, 2299, 2619, 3002
TaiI ACGT 6 cut(s) 100, 1606, 1777, 2058, 2127, 2443
TaqI TCGA 7 cut(s) 832, 910, 924, 1691, 2283, 2511, 3292
TaqII GACCGA 1 cut(s) 2115
TatI WGTACW 4 cut(s) 117, 884, 2619, 3244
TfiI GAWTC 9 cut(s) 307, 866, 989, 1400, 1730, 2086, 2513, 2936, 3350
TscAI CASTG 4 cut(s) 544, 2028, 2269, 2952
TseFI GTSAC 7 cut(s) 1042, 1684, 1813, 1986, 2547, 2827, 3137
TseI GCWGC 6 cut(s) 25, 717, 1173, 2656, 3120, 3434
Tsp45I GTSAC 7 cut(s) 1042, 1684, 1813, 1986, 2547, 2827, 3137
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 4 cut(s) 544, 2028, 2269, 2952
Tth111I GACNNNGTC 1 cut(s) 2972
Vha464I CTTAAG 1 cut(s) 2183
VneI GTGCAC 3 cut(s) 729, 1259, 2169
VpaK11BI GGWCC 1 cut(s) 2098
XagI CCTNNNNNAGG 1 cut(s) 1548
XapI RAATTY 7 cut(s) 140, 956, 1115, 2036, 2212, 2327, 2591
XceI RCATGY 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 1624
XhoI CTCGAG 1 cut(s) 909
XmiI GTMKAC 2 cut(s) 924, 3322
XmnI GAANNNNTTC 1 cut(s) 140
ZraI GACGTC 1 cut(s) 2056
ZrmI AGTACT 2 cut(s) 886, 2621
Zsp2I ATGCAT 3 cut(s) 499, 2136, 3197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.