MD05G1031400.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000143
Physical Location & Seq
Forward (+)
5091477 .. 5096066
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1031400.v1.1.491

Sequence Viewer

Length: 2676 bp
ATGCAGGGCGAAACTTCTTCTCGAGGTAGTGGGGTTGGCTCAGAATGTGCAACGGGCTACACAAGTGAATTTAGGAGTAATGAGGACAACAATTCTGCTCGAGATAGTGGGGTTATCTCAGACTGTGTAATGGATTTCACAAGTGAATTTACTACTTATGCGATATTTAAGACTAGAGAGTCCTTGATGCAGTGGGCTCGTCGGACGGGCAAGCGAAATGGATTTGTTATTGTGACATTACGATCTGATATGGATTGTAAGAGTACAAAACCAAGAGTTACATTAGGTTGTGAGAGGGGTGGAAGATTTAAGGAGAAGAAAACTAGGGCTGCCAAAAAAAGACGTGGGACAGGCACAAAGAAGTGTGGGTGTCCTTTCACATTGAAAGGGGAGAAGTCAGCGAATGGGGACGGATGGATGCTAAAGGTTGTATGTGGAGTGCATAACCATGCATCCACTGAGTATCCTGATGGGCGTTCATATGCAGGGAGGTTATCGGAGCAGGAGGCTTCACTCTTGATAGACTTGTCAAAGAATTTAGTACGACCCAAAGAAATCCTGAGCACATTGAAGCAAAGGGATGAACTCAATGCTACAACCATAAGGACGATCTATAATGCACGCCAAAGGCAAAGAGTTAAAGAGAAGGCGGGAAGGTCTCAAATGCAACAACTGTTATGTAAGTTGAATGAGCATGACTATGTCGAGTGGCATAGGAGGTGCCCTATGTCAGATAGTGTGCAGGACTTGTTTTGGGCGCATCCTGTGAGTTTGGAGTTGTTACGTGCATTTCCAACCATTTTGATGATAGATTGCACGTACAAGACCAATAGGTATTATTATCCCCTACTAGAGGTTGTAGGCGTCGCATCCACTGATATTACATTCTCAATTGCATTTGTCTTTTTGAATTCTGAAAAAGAAGACAACTACATATGGGCATTAAGTAAATTGCGTGGTGTCATGGTGGAGTGTCCCATGCCAAATGTAATTGTCACAGACATGGATTTGCAATTGATGAGAGCCATTGAGGTAGTATTCCCTACCGCTAAAAATCTTTTATGTAAATGGCATATTAATAAGCATGTGTTGGCCAAATGCAAAAACTTGTTTGAGTCCAAGGAAAGGTGTGATAGATTTATTATGAAGTGGAATGTACTGGTGACCTCTCCTACAGAAAATGATTACATACGGGAGCTGGCAGTGTTACAGAGAGAATTTAGTATGTACACTGAGGTCATGGAATATGTTACAAACACATGGTTGAATCCTTTCAAGGACAGATTTGTGTCGGCTTGGACGGACATGATCATGCATTTTGGTAATGTAACATCAAACAGGGCCAAGGGTGCACATACCAAGCTTAAGAGGGAACTTGGTATGAGCCTGGGAAATTTTGAGGGTTCTTTTGAGAGTATACACTCTCTTATTGAGTTACAGCACACTGACATAAAGATATCTTTTGACAAGTGTTTGATGGTCACACAACAAAGCTTCAAGTCTTCAGAATTCAAATATCTGAGAGGAGTTGTATCTATGTTTGCATTGGAGAAGGTGTTATCTCAGTCCAAACTAGCCACCTCAATTGGTGTTGATGGTTCAGGATGTGGTTGTGTCATACCCCGAACCCATGGTCTACCTTGTGCCCATCAGATTGTGAAATACAAGAGGGAGTGTCAGCCAATTCCTCTTGAGTCTCTTGACCCCCATTGGACGAAACTTGATATACTACCTCCTAGTTCTGCTCAGGATTCTATGGATGTGAATGAGTTTCCTGAGTTGGAGTTGCTTCAACAATACTTTCTGGAGGCAAATGCAGAAAATAGGTTGATGCTCAGGAAAAGAGTGAGAGAACTTGTAGCACCCTATACAACTAATCTTGTTGAACCAGGAGTTAAGTCCAGATTACGAGGTCAACTGAGGGAGAAAATTGATATGTCCACTCAACAAGATCCATATGCATTTGATTTGAGATCTGCATCTGTGGATAGTCACTCACCTAGCCCAATGGATGGTCAGCAAAATTTTACACACAAGCAAATGGAGCAAACCAAGAAGAAGGTGTACCGGACGCTGTCAATGAAAGGTATTTCGTATATTGATGCATTCCCAATTGGATTGAGGCCCTTCATTCAGCAAGTCAAGGATGTATGTCAGGATGGTCACTGCGGGTTTAGGGCAGTTGCTGATTTAGTGGGAATGGGTGAGAATGGATACTTGCAAGTGCGTAATGACTTGCTTACTGAGTTGTCTTCATTTTCTGCACATTATGGCGAGTTGTATGGCAGCACAGACAGGGTTCGTGAGTTAGCACAGATACTATCATTCTTTGATGAAGGTAGAGCACCATTTGATAGATGGATGATGATGCCTGACATGGGTCATCTTATAGCGTCATGCTATAAGGTCGTGCTTTTCCACTTATCATCTACTCAGTGCCTAACCTTCTTACCACTTCGGTCGGCACCAGTTCCTTTGCCAAGGCGTCGACAGATTGCTATTGGTTTTATGAATGATTGTCATTGCGTGGAGGTGTTCTTAAGGCCAGGGCATCCTGTGCCTCCCATTACGTCTGATTGGATCAGATATCATACACCAGCTGCACAAGGATGGGACACTGTGTATACTAGTTGTATTTTACGCTTCAAATCTCTTGTCAGCTCTGATCAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
FAR1 MULE SET

Protein Analysis

892

Amino Acids

101.63

Weight (kDa)

8.81

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZSWIM1-3_RNaseH-like PF21056 258 - 353 3e-10 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 268 - 361 4.8e-23 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76710 AT1G76710 AT1G76710 AT1G76710
fragaria_vesca FvH4_1g20063 FvH4_2g15231 FvH4_3g04011 FvH4_3g28681 FvH4_4g01411 FvH4_4g10181 FvH4_4g15922 FvH4_4g16651 FvH4_4g16652 FvH4_5g13892 FvH4_5g13893 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g20623 FvH4_5g25511 FvH4_6g12551 FvH4_6g12552 FvH4_6g38841 FvH4_7g04391
malus_domestica MD05G1031300.v1.1 MD05G1031400.v1.1 MD05G1031500.v1.1 MD08G1164400.v1.1 MD10G1032900.v1.1 MD10G1033000.v1.1 MD10G1033100.v1.1
prunus_persica Prupe.3G240700_v2.0.a1 Prupe.7G144600_v2.0.a1 Prupe.8G038500_v2.0.a1 Prupe.8G038500_v2.0.a1
pyrus_communis pycom05g02250 pycom05g02260 pycom05g02270 pycom05g02280 pycom08g14160 pycom10g02100 pycom10g02110 pycom10g02130
rosa_chinensis RchiOBHm_Chr1g0329481 RchiOBHm_Chr1g0329491 RchiOBHm_Chr1g0341531 RchiOBHm_Chr3g0464811 RchiOBHm_Chr3g0494701 RchiOBHm_Chr3g0497501 RchiOBHm_Chr4g0385841 RchiOBHm_Chr5g0061181 RchiOBHm_Chr6g0263001 RchiOBHm_Chr7g0193111 RchiOBHm_Chr7g0206281 RchiOBHm_Chr7g0206311
rosa_laevigata RLG00000018908 RLG00000028528 RLG00000028803 RLG00000029882 RLG00000029883 RLG00000030361
rosa_multiflora Rmu_co8196328.1_g000001 Rmu_co8259135.1_g000001 Rmu_co8269185.1_g000001 Rmu_sc0000076.1_g000019 Rmu_sc0000311.1_g000007 Rmu_sc0000429.1_g000038 Rmu_sc0000429.1_g000039 Rmu_sc0000429.1_g000040 Rmu_sc0000511.1_g000005 Rmu_sc0000539.1_g000054 Rmu_sc0000566.1_g000039 Rmu_sc0000805.1_g000032 Rmu_sc0000870.1_g000073 Rmu_sc0000870.1_g000074 Rmu_sc0001010.1_g000018 Rmu_sc0001083.1_g000013 Rmu_sc0001114.1_g000011 Rmu_sc0001702.1_g000027 Rmu_sc0001779.1_g000012 Rmu_sc0002923.1_g000036 Rmu_sc0003242.1_g000011 Rmu_sc0003291.1_g000036 Rmu_sc0003393.1_g000012 Rmu_sc0003872.1_g000016 Rmu_sc0003872.1_g000017 Rmu_sc0003872.1_g000018 Rmu_sc0004084.1_g000020 Rmu_sc0004386.1_g000005 Rmu_sc0004622.1_g000005 Rmu_sc0004742.1_g000021 Rmu_sc0004755.1_g000020 Rmu_sc0004805.1_g000036 Rmu_sc0005065.1_g000006 Rmu_sc0005177.1_g000004 Rmu_sc0006373.1_g000003 Rmu_sc0006373.1_g000004 Rmu_sc0007121.1_g000002 Rmu_sc0007943.1_g000003 Rmu_sc0010368.1_g000006 Rmu_sc0010543.1_g000001 Rmu_sc0012591.1_g000006 Rmu_sc0013504.1_g000009 Rmu_sc0015025.1_g000001 Rmu_sc0016170.1_g000001 Rmu_sc0017156.1_g000001 Rmu_sc0026439.1_g000001
rosa_roxburghii Rroxscaffold_175G00432280 Rroxscaffold_1G00030190 Rroxscaffold_1G00030880 Rroxscaffold_2G00090520 Rroxscaffold_3G00227940 Rroxscaffold_3G00233400 Rroxscaffold_3G00238500 Rroxscaffold_3G00251650 Rroxscaffold_4G00301570 Rroxscaffold_4G00321040 Rroxscaffold_5G00350880 Rroxscaffold_7G00184400
rosa_rugosa Rorug01G0080800 Rorug01G0080900 Rorug01G0193900 Rorug02G0275900 Rorug02G0275900 Rorug02G0275900 Rorug02G0305400 Rorug02G0311800 Rorug02G0350400 Rorug02G0381300 Rorug03G0136700 Rorug03G0181900 Rorug03G0268000 Rorug04G0301600 Rorug05G0175100 Rorug05G0250700 Rorug05G0298200 Rorug05G0596000 Rorug06G0029500 Rorug07G0094300 Rorug07G0094300 Rorug07G0095400 Rorug07G0140100
rosa_samantha Rh1AG099200 Rh1AG190500 Rh1BG078900 Rh1BG079000 Rh1BG079100 Rh1CG084600 Rh1CG084700 Rh1CG095300 Rh1CG175900 Rh1DG103300 Rh1DG150400 Rh2AG079300 Rh2AG272400 Rh2AG464800 Rh2AG507400 Rh2DG078300 Rh2DG078400 Rh3CG206900 Rh3CG295400 Rh3DG206800 Rh3DG356200 Rh4CG071500 Rh4CG179200 Rh4DG064700 Rh5CG362400 Rh7AG212300 Rh7AG226200 Rh7BG222200
rosa_wichuraiana Rw0G009990 Rw0G011260 Rw0G011270 Rw0G012910 Rw0G013740 Rw1G007740 Rw1G013050 Rw1G014230 Rw2G014270 Rw3G012720 Rw3G028110 Rw4G006630 Rw4G015270 Rw4G016160 Rw4G031380 Rw5G011440 Rw5G029180 Rw5G048360 Rw5G048430 Rw5G048780 Rw6G005250 Rw6G006390 Rw7G002840 Rw7G014080 Rw7G019650 Rw7G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 178
AccB1I GGYRCC 2 cut(s) 720, 2464
AccB7I CCANNNNNTGG 1 cut(s) 2012
AccI GTMKAC 4 cut(s) 1417, 1636, 2488, 2624
AciI CCGC 3 cut(s) 650, 1047, 2169
AclWI GGATC 2 cut(s) 1946, 2588
AcoI YGGCCR 1 cut(s) 1092
AcsI RAATTY 8 cut(s) 68, 146, 535, 910, 1217, 1393, 1508, 2023
AcuI CTGAAG 1 cut(s) 1488
AcyI GRCGYC 2 cut(s) 864, 2485
AfaI GTAC 6 cut(s) 265, 543, 821, 1158, 1229, 2066
AfiI CCNNNNNNNGG 4 cut(s) 853, 1125, 2012, 2378
AflII CTTAAG 2 cut(s) 1364, 2539
AhlI ACTAGT 1 cut(s) 2627
AjiI CACGTC 1 cut(s) 344
AjnI CCWGG 3 cut(s) 1386, 1888, 2545
AluBI AGCT 5 cut(s) 1198, 1363, 1494, 2600, 2661
AluI AGCT 5 cut(s) 1198, 1363, 1494, 2600, 2661
Alw21I GWGCWC 3 cut(s) 566, 1354, 2347
Alw26I GTCTC 2 cut(s) 663, 1701
Alw44I GTGCAC 1 cut(s) 1350
AlwI GGATC 2 cut(s) 1946, 2588
AlwNI CAGNNNCTG 1 cut(s) 2186
Ama87I CYCGRG 2 cut(s) 21, 99
AoxI GGCC 4 cut(s) 1092, 1341, 2123, 2543
ApaLI GTGCAC 1 cut(s) 1350
ApeKI GCWGC 3 cut(s) 329, 2286, 2600
ApoI RAATTY 8 cut(s) 68, 146, 535, 910, 1217, 1393, 1508, 2023
ArsI GACNNNNNNTTYG 4 cut(s) 1618, 1650, 2640, 2672
AseI ATTAAT 1 cut(s) 1077
Asp700I GAANNNNTTC 1 cut(s) 1271
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 2 cut(s) 1341, 2124
AsuHPI GGTGA 3 cut(s) 1174, 1989, 2216
AvaI CYCGRG 2 cut(s) 21, 99
BaeGI GKGCMC 3 cut(s) 725, 1354, 1648
BalI TGGCCA 1 cut(s) 1094
BanI GGYRCC 2 cut(s) 720, 2464
BanII GRGCYC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 2048, 2080
BbsI GAAGAC 3 cut(s) 930, 1494, 2244
Bbv12I GWGCWC 3 cut(s) 566, 1354, 2347
BbvI GCAGC 3 cut(s) 316, 2298, 2587
BccI CCATC 9 cut(s) 408, 464, 1471, 1589, 1656, 2006, 2153, 2352, 2604
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 3 cut(s) 1388, 1890, 2547
BciVI GTATCC 2 cut(s) 474, 2207
BclI TGATCA 2 cut(s) 1308, 2665
BcoDI GTCTC 2 cut(s) 663, 1701
BcuI ACTAGT 1 cut(s) 2627
BfaI CTAG 7 cut(s) 174, 324, 851, 1574, 1737, 2001, 2628
BfmI CTRYAG 1 cut(s) 1173
BfrI CTTAAG 2 cut(s) 1364, 2539
BfuI GTATCC 2 cut(s) 474, 2207
BglII AGATCT 1 cut(s) 1973
BisI GCNGC 3 cut(s) 330, 2287, 2601
BlsI GCNGC 3 cut(s) 331, 2288, 2602
Bme1390I CCNGG 3 cut(s) 1388, 1890, 2547
BmeT110I CYCGRG 2 cut(s) 21, 99
BmgBI CACGTC 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 1341, 2124
BmiI GGNNCC 2 cut(s) 722, 2466
BmrFI CCNGG 3 cut(s) 1388, 1890, 2547
BoxI GACNNNNGTC 1 cut(s) 2379
BpiI GAAGAC 3 cut(s) 930, 1494, 2244
BpmI CTGGAG 1 cut(s) 1826
Bpu10I CCTNAGC 3 cut(s) 560, 1746, 1835
BpuEI CTTGAG 1 cut(s) 1712
BsaAI YACGTR 2 cut(s) 785, 819
BsaBI GATNNNNATC 1 cut(s) 1978
BsaHI GRCGYC 2 cut(s) 864, 2485
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 6 cut(s) 1119, 1344, 1387, 1630, 2480, 2546
BsaWI WCCGGW 1 cut(s) 2067
BsaXI ACNNNNNCTCC 2 cut(s) 1541, 1571
Bsc4I CCNNNNNNNGG 4 cut(s) 853, 1125, 2012, 2378
Bse1I ACTGG 2 cut(s) 1164, 2468
Bse3DI GCAATG 1 cut(s) 2521
Bse8I GATNNNNATC 1 cut(s) 1978
BseBI CCWGG 3 cut(s) 1388, 1890, 2547
BseDI CCNNGG 6 cut(s) 1119, 1344, 1387, 1630, 2480, 2546
BseJI GATNNNNATC 1 cut(s) 1978
BseLI CCNNNNNNNGG 4 cut(s) 853, 1125, 2012, 2378
BseMI GCAATG 1 cut(s) 2521
BseNI ACTGG 2 cut(s) 1164, 2468
BseRI GAGGAG 1 cut(s) 1539
BseSI GKGCMC 3 cut(s) 725, 1354, 1648
BseXI GCAGC 3 cut(s) 316, 2298, 2587
BsgI GTGCAG 3 cut(s) 761, 2247, 2586
Bsh1285I CGRYCG 1 cut(s) 2462
BshFI GGCC 4 cut(s) 1094, 1343, 2125, 2545
BshNI GGYRCC 2 cut(s) 720, 2464
BsiEI CGRYCG 1 cut(s) 2462
BsiHKAI GWGCWC 3 cut(s) 566, 1354, 2347
BsiHKCI CYCGRG 2 cut(s) 21, 99
BsiSI CCGG 1 cut(s) 2068
BslFI GGGAC 4 cut(s) 361, 422, 960, 2627
BslI CCNNNNNNNGG 4 cut(s) 853, 1125, 2012, 2378
BsmAI GTCTC 2 cut(s) 663, 1701
BsmFI GGGAC 4 cut(s) 361, 422, 960, 2627
BsmI GAATGC 1 cut(s) 2105
BsnI GGCC 4 cut(s) 1094, 1343, 2125, 2545
Bso31I GGTCTC 1 cut(s) 663
BsoBI CYCGRG 2 cut(s) 21, 99
Bsp1286I GDGCHC 6 cut(s) 199, 566, 725, 1354, 1648, 2347
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
Bsp19I CCATGG 1 cut(s) 1630
BspACI CCGC 3 cut(s) 650, 1047, 2169
BspANI GGCC 4 cut(s) 1094, 1343, 2125, 2545
BspLI GGNNCC 2 cut(s) 722, 2466
BspPI GGATC 2 cut(s) 1946, 2588
BspT107I GGYRCC 2 cut(s) 720, 2464
BspTI CTTAAG 2 cut(s) 1364, 2539
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 2521
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 2 cut(s) 1164, 2468
BssECI CCNNGG 6 cut(s) 1119, 1344, 1387, 1630, 2480, 2546
BssMI GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
BssNAI GTATAC 2 cut(s) 1418, 2625
BssNI GRCGYC 2 cut(s) 864, 2485
BssT1I CCWWGG 4 cut(s) 1119, 1344, 1630, 2480
Bst1107I GTATAC 2 cut(s) 1418, 2625
Bst2UI CCWGG 3 cut(s) 1388, 1890, 2547
Bst4CI ACNGT 3 cut(s) 125, 675, 2620
BstACI GRCGYC 2 cut(s) 864, 2485
BstAFI CTTAAG 2 cut(s) 1364, 2539
BstAPI GCANNNNNTGC 1 cut(s) 2557
BstAUI TGTACA 1 cut(s) 1227
BstBAI YACGTR 2 cut(s) 785, 819
BstC8I GCNNGC 3 cut(s) 212, 622, 1200
BstDSI CCRYGG 1 cut(s) 1630
BstEII GGTNACC 1 cut(s) 1162
BstENI CCTNNNNNAGG 1 cut(s) 851
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 7 cut(s) 245, 612, 1311, 1954, 1976, 2583, 2668
BstMAI GTCTC 2 cut(s) 663, 1701
BstMBI GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
BstMCI CGRYCG 1 cut(s) 2462
BstMWI GCNNNNNNNGC 3 cut(s) 1349, 2044, 2557
BstNI CCWGG 3 cut(s) 1388, 1890, 2547
BstNSI RCATGY 1 cut(s) 1088
BstPAI GACNNNNGTC 1 cut(s) 2379
BstPI GGTNACC 1 cut(s) 1162
BstSCI CCNGG 3 cut(s) 1386, 1888, 2545
BstSFI CTRYAG 1 cut(s) 1173
BstSLI GKGCMC 3 cut(s) 725, 1354, 1648
BstV1I GCAGC 3 cut(s) 316, 2298, 2587
BstV2I GAAGAC 3 cut(s) 930, 1494, 2244
BstX2I RGATCY 2 cut(s) 1951, 1973
BstYI RGATCY 2 cut(s) 1951, 1973
BstZ17I GTATAC 2 cut(s) 1418, 2625
BsuI GTATCC 2 cut(s) 474, 2207
BsuRI GGCC 4 cut(s) 1094, 1343, 2125, 2545
BtgI CCRYGG 1 cut(s) 1630
BtrI CACGTC 1 cut(s) 344
BtsI GCAGTG 3 cut(s) 197, 1209, 2164
BtsIMutI CAGTG 9 cut(s) 197, 456, 873, 1209, 1230, 1443, 2164, 2441, 2616
Cac8I GCNNGC 3 cut(s) 212, 622, 1200
CaiI CAGNNNCTG 1 cut(s) 2186
CfoI GCGC 1 cut(s) 760
Cfr13I GGNCC 2 cut(s) 1341, 2124
CseI GACGC 4 cut(s) 853, 2080, 2382, 2474
Csp6I GTAC 6 cut(s) 264, 542, 820, 1157, 1228, 2065
CspCI CAANNNNNGTGG 2 cut(s) 1567, 1602
CviQI GTAC 6 cut(s) 264, 542, 820, 1157, 1228, 2065
DpnI GATC 7 cut(s) 244, 611, 1310, 1953, 1975, 2582, 2667
DpnII GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
DrdI GACNNNNNNGTC 1 cut(s) 178
DseDI GACNNNNNNGTC 1 cut(s) 178
EaeI YGGCCR 1 cut(s) 1092
Eco130I CCWWGG 4 cut(s) 1119, 1344, 1630, 2480
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 663
Eco32I GATATC 2 cut(s) 1458, 2588
Eco57I CTGAAG 1 cut(s) 1488
Eco88I CYCGRG 2 cut(s) 21, 99
Eco91I GGTNACC 1 cut(s) 1162
EcoNI CCTNNNNNAGG 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 2124
EcoO65I GGTNACC 1 cut(s) 1162
EcoRI GAATTC 2 cut(s) 910, 1508
EcoRII CCWGG 3 cut(s) 1386, 1888, 2545
EcoRV GATATC 2 cut(s) 1458, 2588
EcoT14I CCWWGG 4 cut(s) 1119, 1344, 1630, 2480
EcoT22I ATGCAT 4 cut(s) 454, 1317, 1963, 2107
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 4 cut(s) 1119, 1344, 1630, 2480
FaqI GGGAC 4 cut(s) 361, 422, 960, 2627
FauI CCCGC 2 cut(s) 643, 2162
FauNDI CATATG 3 cut(s) 481, 935, 1957
FbaI TGATCA 2 cut(s) 1308, 2665
FblI GTMKAC 4 cut(s) 1417, 1636, 2488, 2624
Fnu4HI GCNGC 3 cut(s) 330, 2287, 2601
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 3 cut(s) 330, 2287, 2601
FspBI CTAG 7 cut(s) 174, 324, 851, 1574, 1737, 2001, 2628
GlaI GCGC 1 cut(s) 759
GluI GCNGC 3 cut(s) 330, 2287, 2601
GsuI CTGGAG 1 cut(s) 1826
HaeIII GGCC 4 cut(s) 1094, 1343, 2125, 2545
HapII CCGG 1 cut(s) 2068
HgaI GACGC 4 cut(s) 853, 2080, 2382, 2474
HhaI GCGC 1 cut(s) 760
Hin1I GRCGYC 2 cut(s) 864, 2485
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HincII GTYRAC 2 cut(s) 1916, 2489
HindII GTYRAC 2 cut(s) 1916, 2489
HindIII AAGCTT 2 cut(s) 1361, 1492
HinfI GANTC 5 cut(s) 179, 1115, 1267, 1694, 1751
HpaII CCGG 1 cut(s) 2068
HphI GGTGA 3 cut(s) 1174, 1989, 2216
Hpy166II GTNNAC 9 cut(s) 1230, 1352, 1418, 1637, 1916, 1941, 2065, 2489, 2625
Hpy8I GTNNAC 9 cut(s) 1230, 1352, 1418, 1637, 1916, 1941, 2065, 2489, 2625
Hpy99I CGWCG 3 cut(s) 204, 869, 2490
HpyAV CCTTC 7 cut(s) 640, 648, 1546, 2053, 2137, 2330, 2455
HpyCH4III ACNGT 3 cut(s) 125, 675, 2620
HpyCH4IV ACGT 4 cut(s) 343, 784, 818, 2570
HpyF10VI GCNNNNNNNGC 3 cut(s) 1349, 2044, 2557
HpySE526I ACGT 4 cut(s) 343, 784, 818, 2570
Hsp92I GRCGYC 2 cut(s) 864, 2485
HspAI GCGC 1 cut(s) 758
Ksp22I TGATCA 2 cut(s) 1308, 2665
Kzo9I GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
LmnI GCTCC 3 cut(s) 499, 1195, 2044
Lsp1109I GCAGC 3 cut(s) 316, 2298, 2587
MaeI CTAG 7 cut(s) 174, 324, 851, 1574, 1737, 2001, 2628
MaeII ACGT 4 cut(s) 343, 784, 818, 2570
MalI GATC 7 cut(s) 244, 611, 1310, 1953, 1975, 2582, 2667
MboI GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
MboII GAAGA 7 cut(s) 9, 315, 328, 935, 1494, 2068, 2244
MfeI CAATTG 4 cut(s) 891, 1013, 1584, 2112
MflI RGATCY 2 cut(s) 1951, 1973
MhlI GDGCHC 6 cut(s) 199, 566, 725, 1354, 1648, 2347
MlsI TGGCCA 1 cut(s) 1094
MluNI TGGCCA 1 cut(s) 1094
MlyI GAGTC 3 cut(s) 188, 1124, 1703
MmeI TCCRAC 3 cut(s) 182, 818, 1761
Mox20I TGGCCA 1 cut(s) 1094
Mph1103I ATGCAT 4 cut(s) 454, 1317, 1963, 2107
MroXI GAANNNNTTC 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 1094
MseI TTAA 8 cut(s) 168, 309, 639, 944, 1077, 1365, 1896, 2540
MslI CAYNNNNRTG 6 cut(s) 447, 699, 803, 1001, 1310, 2608
Msp20I TGGCCA 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 2600
MspCI CTTAAG 2 cut(s) 1364, 2539
MspI CCGG 1 cut(s) 2068
MspR9I CCNGG 3 cut(s) 1388, 1890, 2547
MunI CAATTG 4 cut(s) 891, 1013, 1584, 2112
Mva1269I GAATGC 1 cut(s) 2105
MvaI CCWGG 3 cut(s) 1388, 1890, 2547
MwoI GCNNNNNNNGC 3 cut(s) 1349, 2044, 2557
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 3 cut(s) 481, 935, 1957
NdeII GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
NlaIV GGNNCC 2 cut(s) 722, 2466
NmuCI GTSAC 6 cut(s) 232, 994, 1162, 1480, 1991, 2162
NsiI ATGCAT 4 cut(s) 454, 1317, 1963, 2107
NspI RCATGY 1 cut(s) 1088
PaeR7I CTCGAG 2 cut(s) 21, 99
PctI GAATGC 1 cut(s) 2105
PdmI GAANNNNTTC 1 cut(s) 1271
PfeI GAWTC 2 cut(s) 1267, 1751
PflFI GACNNNGTC 2 cut(s) 701, 2074
PflMI CCANNNNNTGG 1 cut(s) 2012
PkrI GCNGC 3 cut(s) 331, 2288, 2602
PleI GAGTC 3 cut(s) 187, 1123, 1702
PpsI GAGTC 3 cut(s) 187, 1123, 1702
Ppu21I YACGTR 2 cut(s) 785, 819
PshAI GACNNNNGTC 1 cut(s) 2379
PshBI ATTAAT 1 cut(s) 1077
Psp6I CCWGG 3 cut(s) 1386, 1888, 2545
PspEI GGTNACC 1 cut(s) 1162
PspGI CCWGG 3 cut(s) 1386, 1888, 2545
PspN4I GGNNCC 2 cut(s) 722, 2466
PspPI GGNCC 2 cut(s) 1341, 2124
PstNI CAGNNNCTG 1 cut(s) 2186
PsuI RGATCY 2 cut(s) 1951, 1973
PsyI GACNNNGTC 2 cut(s) 701, 2074
PvuII CAGCTG 1 cut(s) 2600
RsaI GTAC 6 cut(s) 265, 543, 821, 1158, 1229, 2066
RsaNI GTAC 6 cut(s) 264, 542, 820, 1157, 1228, 2065
RseI CAYNNNNRTG 6 cut(s) 447, 699, 803, 1001, 1310, 2608
SalI GTCGAC 1 cut(s) 2487
SaqAI TTAA 8 cut(s) 168, 309, 639, 944, 1077, 1365, 1896, 2540
SatI GCNGC 3 cut(s) 330, 2287, 2601
Sau3AI GATC 7 cut(s) 242, 609, 1308, 1951, 1973, 2580, 2665
Sau96I GGNCC 2 cut(s) 1341, 2124
SchI GAGTC 3 cut(s) 188, 1124, 1703
ScrFI CCNGG 3 cut(s) 1388, 1890, 2547
SduI GDGCHC 6 cut(s) 199, 566, 725, 1354, 1648, 2347
SfcI CTRYAG 1 cut(s) 1173
Sfr274I CTCGAG 2 cut(s) 21, 99
SlaI CTCGAG 2 cut(s) 21, 99
SmiMI CAYNNNNRTG 6 cut(s) 447, 699, 803, 1001, 1310, 2608
SmlI CTYRAG 5 cut(s) 21, 99, 1364, 1691, 2539
SmoI CTYRAG 5 cut(s) 21, 99, 1364, 1691, 2539
SpeI ACTAGT 1 cut(s) 2627
SsiI CCGC 3 cut(s) 650, 1047, 2169
SspMI CTAG 7 cut(s) 174, 324, 851, 1574, 1737, 2001, 2628
StyD4I CCNGG 3 cut(s) 1386, 1888, 2545
StyI CCWWGG 4 cut(s) 1119, 1344, 1630, 2480
TaaI ACNGT 3 cut(s) 125, 675, 2620
TaiI ACGT 4 cut(s) 346, 787, 821, 2573
TaqI TCGA 4 cut(s) 22, 100, 705, 2488
TaqII GACCGA 1 cut(s) 2448
TatI WGTACW 3 cut(s) 263, 1156, 1227
TfiI GAWTC 2 cut(s) 1267, 1751
Tru1I TTAA 8 cut(s) 168, 309, 639, 944, 1077, 1365, 1896, 2540
Tru9I TTAA 8 cut(s) 168, 309, 639, 944, 1077, 1365, 1896, 2540
TscAI CASTG 9 cut(s) 197, 463, 880, 1209, 1237, 1450, 2171, 2441, 2623
TseFI GTSAC 6 cut(s) 232, 994, 1162, 1480, 1991, 2162
TseI GCWGC 3 cut(s) 329, 2286, 2600
Tsp45I GTSAC 6 cut(s) 232, 994, 1162, 1480, 1991, 2162
TspDTI ATGAA 8 cut(s) 468, 597, 1160, 2096, 2119, 2244, 2349, 2525
TspGWI ACGGA 2 cut(s) 426, 1316
TspRI CASTG 9 cut(s) 197, 463, 880, 1209, 1237, 1450, 2171, 2441, 2623
Tth111I GACNNNGTC 2 cut(s) 701, 2074
Van91I CCANNNNNTGG 1 cut(s) 2012
Vha464I CTTAAG 2 cut(s) 1364, 2539
VneI GTGCAC 1 cut(s) 1350
VspI ATTAAT 1 cut(s) 1077
XagI CCTNNNNNAGG 1 cut(s) 851
XapI RAATTY 8 cut(s) 68, 146, 535, 910, 1217, 1393, 1508, 2023
XceI RCATGY 1 cut(s) 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 2355
XhoI CTCGAG 2 cut(s) 21, 99
XmiI GTMKAC 4 cut(s) 1417, 1636, 2488, 2624
XmnI GAANNNNTTC 1 cut(s) 1271
XspI CTAG 7 cut(s) 174, 324, 851, 1574, 1737, 2001, 2628
Zsp2I ATGCAT 4 cut(s) 454, 1317, 1963, 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.