FvH4_3g15130

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000096
Physical Location & Seq
Forward (+)
9383805 .. 9386796
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15130.t1

Sequence Viewer

Length: 2406 bp
ATGATTAACAGCATTGCAATGATCAACTACTTGTTGTGCTTGCTCATCAGTTTTTCAATCTTGTGGACTAGTCGTGGTGCACCATTGTTCATTGGGGACACGCTTAGGGCAGGGGAGGCTCTCAATTCCTCGAGCTTTATGGTCTCCGCTTCGGGGGAGTTCACGTTGGGTTTCTACTCCGGCTACCTCACAGTAAGGTCCAACAACTACACGGTCTGGGTTGCCAACCTGGTCTCACCGATTAACGACACTTCGTCGGCGCTTCTCACTTTGTTGGACATGAACAAAACTCTCCTGCAAATAATGGAGACAGGTGAAAAACCTGTCGTTCTTTACTCCTCTCCGTTTATTATTAGTACCAATGTCGTGGCTACCCTGACGGATTCTGGCAGTTTAGTCCTACAAGAGGTGGGTCAGAATGTTTCAAACTCTAATGTGCTGTGGCAAAGCATTGATGAACCTAATGTGGCACTTATGCCGGGGATGAAATTGAAAAGTAGTGATGAGATATACTCTTGGCAGGAAAGTCAGACCAGTCCGGGGATTGGGTCTCTCAGCCTCCAATGGGATTCTGACAAACTCCAACTGCAAGTTTGGAGGGACAATGCTAATTGCTGGAGTAGCGGAGGCCGATTCAGTATGGGGGGATTTGAGTTTATTAATATCCCACATGAATCCAAGGTTAGATACAATTTTAGCGTGGTTTCGAATAGCAATGAAAACTATTTCAGTTACGCTGTCGTTGGTGATCAAGATGTTAGATCAGCTTGGGTGCTACACACAGATGGGGAGCTTTATGATGTTTACAATAACATTTCTATTGTGCGAGCAGGTGACTGTGATGGCTTTAACACCAATGGAGGGTGCGTGAGATGGGGCCCCGCCATGTCCGATTACTGTGGTTATAGCTTTTCATTCATTAATGATTTTGTGATGATAAAACATGGTTCCTTCAAACCAATCAGTATGGCCCCCTCTATCAACTCAACATGGCCGGATTCATATCTTAGGAATGGTGATTGCACGTCTACTTGTTGGAAGGACTGTGACTGCCTTGGTTTCGACTTTGATAATCAGACTGGATGTATATATTGGAGTATAACGTACTTTCACTTCCTTGAAGATCCAGCTAGTTCTACAATTGGTTTCGTTCTACCGAGGAAACCACTTCAAAGAACTAATAGTAAGGACACATCACAAAAGTGGATATGGATTGGTATTTCAATTGCGGTTGTTCTTCTGGCACTGGTGCTTTGCAGCCTGTGCTATTTAATACAAAGAAGAAAACTTGCAGGTGAGAACCAAAGAAAGATCCAGGATATGCTGAACATGATGAATTCTAATAGACCTACCAATGCTAATGGACTCCAAAATGATGGAAAGCGTGGACATGATTTAAGCGTATTTAGTTATGCATCTATTGCAGCTGCGACTTGCAACTTCTCTGATAAGAATAAGCTAGGACAAGGCGGTTTTGGACCTGTTTATAAGGGGAAATTTGTGACCGGACAAGAAATAGCAGTGAAGAGACTTTCAAAATGTTCAGAGCAGGGAATATCAGAGTTTAAGAATGAGTTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGCTCTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAACAGCTTGGACTACTTTTTATTTGGTTATTCAATTAGATCCCAACAGTTAGATTGGGATAGGCGTTTCCGCATAATTGAAGGAATTGCTCAAGGTTTGCTTTACTTGCACAAATATTCCAGAACGAGAATAATTCATAGAGATGTGAAAGCAAGTAATGTTCTACTTGATGAGAGGATGAACCCCAAAATTTCTGATTTTGGAATGGCAAGGATTTTTGCCAATGATGAAGAAGAAGCAAATACAATGAAGATTGTTGGGACACGTGGTTATATGTCCCCTGAGTATGTTATGGGGGGGAATTTTTCCACAAAATCTGATGTCTATAGCTTTGGAGTGTTGATGCTTGAAATCCTTAGTGGTAGGAAAAACAACAGCTTCTACAATGATGATCGCGCCATCAATTTAGTCGGCTATGCATGGGCGTTATGGAGTGAAGGTGCAGGACTAGGACTAATGGATCCAACAATTGGTGATTCATGTGACAAAGATCAGATGTTGAGGTGCATCCATGTTGGTCTGCTATGCGTGAAGGAAAATGCAGCTGATAGACCGACGATGTCAGATGTGATACCAATGCTGACCAATGAAAGTATGTCACTGCCAGTACCTACAAAACCAGCATTTTGTTCTGAAAGAAATGTGATCACAGATGCTGTAGATGGAAACAGACCGGAAATAGTTGCATCTGTAAATGGTATCTCCATTTCTGATTTCGATGGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

802

Amino Acids

89.47

Weight (kDa)

5.25

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 167 3.4e-14 D-mannose binding lectin
Pkinase PF00069 482 - 683 1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 484 - 687 4.8e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1488
AarI CACCTGC 2 cut(s) 821, 1283
Acc36I ACCTGC 2 cut(s) 821, 1283
AccB7I CCANNNNNTGG 1 cut(s) 2147
AccI GTMKAC 1 cut(s) 1028
AccII CGCG 1 cut(s) 2073
AciI CCGC 6 cut(s) 147, 624, 882, 1229, 1470, 1747
AclWI GGATC 5 cut(s) 1118, 1306, 1710, 2132, 2145
AcoI YGGCCR 1 cut(s) 992
AcsI RAATTY 4 cut(s) 1336, 1496, 1866, 1978
AcvI CACGTG 1 cut(s) 1943
AfaI GTAC 4 cut(s) 358, 1106, 1667, 2286
AfiI CCNNNNNNNGG 7 cut(s) 153, 406, 540, 545, 565, 861, 2147
AflIII ACRYGT 1 cut(s) 1940
AhdI GACNNNNNGTC 1 cut(s) 253
AhlI ACTAGT 1 cut(s) 68
AjiI CACGTC 1 cut(s) 1026
AjnI CCWGG 2 cut(s) 228, 1314
AleI CACNNNNGTG 1 cut(s) 1201
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 5 cut(s) 148, 238, 302, 555, 1522
Alw44I GTGCAC 1 cut(s) 78
AlwI GGATC 5 cut(s) 1118, 1306, 1710, 2132, 2145
AlwNI CAGNNNCTG 1 cut(s) 2333
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 4 cut(s) 628, 877, 969, 992
ApaI GGGCCC 1 cut(s) 881
ApaLI GTGCAC 1 cut(s) 78
ApeKI GCWGC 4 cut(s) 1257, 1424, 1427, 2219
ApoI RAATTY 4 cut(s) 1336, 1496, 1866, 1978
AseI ATTAAT 2 cut(s) 660, 921
AspLEI GCGC 2 cut(s) 262, 2075
AspS9I GGNCC 5 cut(s) 198, 877, 878, 970, 1478
AsuC2I CCSGG 2 cut(s) 480, 540
AsuHPI GGTGA 8 cut(s) 228, 326, 758, 845, 1028, 1307, 1652, 2162
AsuII TTCGAA 1 cut(s) 707
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 2 cut(s) 198, 1478
BaeGI GKGCMC 2 cut(s) 82, 881
BamHI GGATCC 1 cut(s) 2137
BanII GRGCYC 1 cut(s) 881
BarI GAAGNNNNNNTAC 2 cut(s) 1097, 1129
BbrPI CACGTG 1 cut(s) 1943
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 4 cut(s) 1269, 1414, 1436, 2231
BccI CCATC 7 cut(s) 779, 836, 867, 1370, 2084, 2333, 2390
BciT130I CCWGG 2 cut(s) 230, 1316
BclI TGATCA 3 cut(s) 21, 748, 2322
BcnI CCSGG 2 cut(s) 480, 540
BcoDI GTCTC 5 cut(s) 148, 238, 302, 555, 1522
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 4 cut(s) 69, 1131, 1460, 2126
BfmI CTRYAG 2 cut(s) 2002, 2334
BfoI RGCGCY 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 821, 1283
BisI GCNGC 4 cut(s) 1258, 1425, 1428, 2220
BlsI GCNGC 4 cut(s) 1259, 1426, 1429, 2221
Bme1390I CCNGG 4 cut(s) 230, 480, 540, 1316
Bme18I GGWCC 2 cut(s) 198, 1478
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 1026
BmgT120I GGNCC 5 cut(s) 198, 877, 878, 970, 1478
BmiI GGNNCC 6 cut(s) 878, 879, 880, 949, 972, 2139
BmrFI CCNGG 4 cut(s) 230, 480, 540, 1316
BmsI GCATC 5 cut(s) 1424, 2010, 2193, 2320, 2372
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 2 cut(s) 637, 1580
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 1752
BpuMI CCSGG 2 cut(s) 480, 540
BsaAI YACGTR 1 cut(s) 1943
BsaBI GATNNNNATC 1 cut(s) 1002
BsaI GGTCTC 3 cut(s) 148, 238, 555
BsaJI CCNNGG 5 cut(s) 479, 539, 678, 1054, 1157
BsaWI WCCGGW 2 cut(s) 1505, 2350
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 852, 882, 2101, 2131
Bsc4I CCNNNNNNNGG 7 cut(s) 153, 406, 540, 545, 565, 861, 2147
Bse1I ACTGG 4 cut(s) 534, 1084, 1251, 2282
Bse3DI GCAATG 3 cut(s) 12, 24, 721
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 230, 1316
BseDI CCNNGG 5 cut(s) 479, 539, 678, 1054, 1157
BseGI GGATG 5 cut(s) 489, 1088, 1656, 1860, 2184
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 7 cut(s) 153, 406, 540, 545, 565, 861, 2147
BseMI GCAATG 3 cut(s) 12, 24, 721
BseMII CTCAG 2 cut(s) 568, 1950
BseNI ACTGG 4 cut(s) 534, 1084, 1251, 2282
BseRI GAGGAG 1 cut(s) 328
BseSI GKGCMC 2 cut(s) 82, 881
BseXI GCAGC 4 cut(s) 1269, 1414, 1436, 2231
BsgI GTGCAG 1 cut(s) 2139
Bsh1236I CGCG 1 cut(s) 2073
BshFI GGCC 4 cut(s) 630, 879, 971, 994
BsiHKAI GWGCWC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 6 cut(s) 180, 479, 539, 995, 1506, 2351
BslFI GGGAC 4 cut(s) 110, 614, 1939, 1951
BslI CCNNNNNNNGG 7 cut(s) 153, 406, 540, 545, 565, 861, 2147
BsmAI GTCTC 5 cut(s) 148, 238, 302, 555, 1522
BsmFI GGGAC 4 cut(s) 110, 614, 1939, 1951
BsmI GAATGC 1 cut(s) 1632
BsnI GGCC 4 cut(s) 630, 879, 971, 994
Bso31I GGTCTC 3 cut(s) 148, 238, 555
BsoBI CYCGRG 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 707
Bsp120I GGGCCC 1 cut(s) 877
Bsp1286I GDGCHC 2 cut(s) 82, 881
BspACI CCGC 6 cut(s) 147, 624, 882, 1229, 1470, 1747
BspANI GGCC 4 cut(s) 630, 879, 971, 994
BspCNI CTCAG 2 cut(s) 567, 1951
BspFNI CGCG 1 cut(s) 2073
BspLI GGNNCC 6 cut(s) 878, 879, 880, 949, 972, 2139
BspMI ACCTGC 2 cut(s) 821, 1283
BspPI GGATC 5 cut(s) 1118, 1306, 1710, 2132, 2145
BspT104I TTCGAA 1 cut(s) 707
BspTNI GGTCTC 3 cut(s) 148, 238, 555
BsrDI GCAATG 3 cut(s) 12, 24, 721
BsrI ACTGG 4 cut(s) 534, 1084, 1251, 2282
BssECI CCNNGG 5 cut(s) 479, 539, 678, 1054, 1157
BssT1I CCWWGG 2 cut(s) 678, 1054
Bst2UI CCWGG 2 cut(s) 230, 1316
Bst4CI ACNGT 6 cut(s) 193, 214, 839, 899, 1046, 1725
Bst6I CTCTTC 2 cut(s) 1520, 1638
BstAPI GCANNNNNTGC 2 cut(s) 1263, 1421
BstBAI YACGTR 1 cut(s) 1943
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 2 cut(s) 41, 828
BstDEI CTNAG 5 cut(s) 104, 554, 1007, 1959, 2033
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 5 cut(s) 489, 1088, 1656, 1860, 2184
BstFNI CGCG 1 cut(s) 2073
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 2 cut(s) 262, 2075
BstMAI GTCTC 5 cut(s) 148, 238, 302, 555, 1522
BstMWI GCNNNNNNNGC 5 cut(s) 116, 621, 1263, 1421, 1783
BstNI CCWGG 2 cut(s) 230, 1316
BstNSI RCATGY 1 cut(s) 1672
BstSCI CCNGG 4 cut(s) 228, 478, 538, 1314
BstSFI CTRYAG 2 cut(s) 2002, 2334
BstSLI GKGCMC 2 cut(s) 82, 881
BstUI CGCG 1 cut(s) 2073
BstV1I GCAGC 4 cut(s) 1269, 1414, 1436, 2231
BstX2I RGATCY 4 cut(s) 1123, 1311, 1715, 2137
BstXI CCANNNNNNTGG 2 cut(s) 367, 1376
BstYI RGATCY 4 cut(s) 1123, 1311, 1715, 2137
BsuRI GGCC 4 cut(s) 630, 879, 971, 994
BtrI CACGTC 1 cut(s) 1026
BtsCI GGATG 5 cut(s) 489, 1088, 1656, 1860, 2184
BtsI GCAGTG 2 cut(s) 1527, 2276
BtsIMutI CAGTG 3 cut(s) 1244, 1527, 2276
BveI ACCTGC 2 cut(s) 821, 1283
Cac8I GCNNGC 2 cut(s) 41, 828
CaiI CAGNNNCTG 1 cut(s) 2333
CfoI GCGC 2 cut(s) 262, 2075
Cfr13I GGNCC 5 cut(s) 198, 877, 878, 970, 1478
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 4 cut(s) 357, 1105, 1666, 2285
CviQI GTAC 4 cut(s) 357, 1105, 1666, 2285
DdeI CTNAG 5 cut(s) 104, 554, 1007, 1959, 2033
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 992
Eam1104I CTCTTC 2 cut(s) 1520, 1638
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 2 cut(s) 1520, 1638
Eco130I CCWWGG 2 cut(s) 678, 1054
Eco24I GRGCYC 1 cut(s) 881
Eco31I GGTCTC 3 cut(s) 148, 238, 555
Eco47I GGWCC 2 cut(s) 198, 1478
Eco72I CACGTG 1 cut(s) 1943
Eco88I CYCGRG 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 877, 878
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 2 cut(s) 228, 1314
EcoT14I CCWWGG 2 cut(s) 678, 1054
EcoT22I ATGCAT 2 cut(s) 1417, 2098
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 2 cut(s) 678, 1054
FalI AAGNNNNNCTT 2 cut(s) 1761, 1793
FaqI GGGAC 4 cut(s) 110, 614, 1939, 1951
FauI CCCGC 1 cut(s) 889
FbaI TGATCA 3 cut(s) 21, 748, 2322
FblI GTMKAC 1 cut(s) 1028
Fnu4HI GCNGC 4 cut(s) 1258, 1425, 1428, 2220
FokI GGATG 5 cut(s) 496, 1095, 1663, 1867, 2171
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 4 cut(s) 1258, 1425, 1428, 2220
FspBI CTAG 4 cut(s) 69, 1131, 1460, 2126
GlaI GCGC 2 cut(s) 261, 2074
GluI GCNGC 4 cut(s) 1258, 1425, 1428, 2220
GsuI CTGGAG 2 cut(s) 637, 1580
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 4 cut(s) 630, 879, 971, 994
HapII CCGG 6 cut(s) 180, 479, 539, 995, 1506, 2351
HhaI GCGC 2 cut(s) 262, 2075
Hin6I GCGC 2 cut(s) 260, 2073
HinP1I GCGC 2 cut(s) 260, 2073
HinfI GANTC 7 cut(s) 383, 569, 633, 674, 998, 1365, 2153
HpaII CCGG 6 cut(s) 180, 479, 539, 995, 1506, 2351
HphI GGTGA 8 cut(s) 228, 326, 758, 845, 1028, 1307, 1652, 2162
Hpy166II GTNNAC 6 cut(s) 66, 80, 162, 805, 1029, 1388
Hpy188III TCNNGA 2 cut(s) 752, 1797
Hpy8I GTNNAC 6 cut(s) 66, 80, 162, 805, 1029, 1388
Hpy99I CGWCG 2 cut(s) 259, 2236
HpyAV CCTTC 5 cut(s) 961, 1033, 1751, 2108, 2203
HpyCH4III ACNGT 6 cut(s) 193, 214, 839, 899, 1046, 1725
HpyCH4IV ACGT 4 cut(s) 164, 1025, 1103, 1942
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 621, 1263, 1421, 1783
HpyF3I CTNAG 5 cut(s) 104, 554, 1007, 1959, 2033
HpySE526I ACGT 4 cut(s) 164, 1025, 1103, 1942
HspAI GCGC 2 cut(s) 260, 2073
Ksp22I TGATCA 3 cut(s) 21, 748, 2322
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 4 cut(s) 1269, 1414, 1436, 2231
LweI GCATC 5 cut(s) 1424, 2010, 2193, 2320, 2372
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 4 cut(s) 69, 1131, 1460, 2126
MaeII ACGT 4 cut(s) 164, 1025, 1103, 1942
MaeIII GTNAC 6 cut(s) 731, 833, 1046, 1501, 2159, 2274
MboII GAAGA 8 cut(s) 1133, 1229, 1293, 1537, 1655, 1919, 1922, 1939
MfeI CAATTG 3 cut(s) 1140, 1224, 2145
MflI RGATCY 4 cut(s) 1123, 1311, 1715, 2137
MhlI GDGCHC 2 cut(s) 82, 881
MlyI GAGTC 1 cut(s) 1359
MmeI TCCRAC 5 cut(s) 225, 255, 607, 1016, 2165
Mph1103I ATGCAT 2 cut(s) 1417, 2098
MseI TTAA 8 cut(s) 6, 243, 660, 849, 921, 1271, 1397, 1566
MslI CAYNNNNRTG 4 cut(s) 17, 783, 1201, 1818
MspA1I CMGCKG 2 cut(s) 1427, 2222
MspI CCGG 6 cut(s) 180, 479, 539, 995, 1506, 2351
MspR9I CCNGG 4 cut(s) 230, 480, 540, 1316
MunI CAATTG 3 cut(s) 1140, 1224, 2145
Mva1269I GAATGC 1 cut(s) 1632
MvaI CCWGG 2 cut(s) 230, 1316
MvnI CGCG 1 cut(s) 2073
MwoI GCNNNNNNNGC 5 cut(s) 116, 621, 1263, 1421, 1783
NciI CCSGG 2 cut(s) 480, 540
NlaIV GGNNCC 6 cut(s) 878, 879, 880, 949, 972, 2139
NmuCI GTSAC 5 cut(s) 833, 1046, 1501, 2159, 2274
NsiI ATGCAT 2 cut(s) 1417, 2098
NspI RCATGY 1 cut(s) 1672
NspV TTCGAA 1 cut(s) 707
OliI CACNNNNGTG 1 cut(s) 1201
PaeR7I CTCGAG 1 cut(s) 130
PaqCI CACCTGC 2 cut(s) 821, 1283
PctI GAATGC 1 cut(s) 1632
PfeI GAWTC 6 cut(s) 383, 569, 633, 674, 998, 2153
PflFI GACNNNGTC 1 cut(s) 2236
PflMI CCANNNNNTGG 1 cut(s) 2147
PfoI TCCNGGA 1 cut(s) 1314
PkrI GCNGC 4 cut(s) 1259, 1426, 1429, 2221
PleI GAGTC 1 cut(s) 1359
PmaCI CACGTG 1 cut(s) 1943
PmlI CACGTG 1 cut(s) 1943
PpsI GAGTC 1 cut(s) 1359
Ppu21I YACGTR 1 cut(s) 1943
PshBI ATTAAT 2 cut(s) 660, 921
PsiI TTATAA 1 cut(s) 1488
Psp6I CCWGG 2 cut(s) 228, 1314
PspCI CACGTG 1 cut(s) 1943
PspGI CCWGG 2 cut(s) 228, 1314
PspN4I GGNNCC 6 cut(s) 878, 879, 880, 949, 972, 2139
PspOMI GGGCCC 1 cut(s) 877
PspPI GGNCC 5 cut(s) 198, 877, 878, 970, 1478
PspXI VCTCGAGB 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 2333
PsuI RGATCY 4 cut(s) 1123, 1311, 1715, 2137
PsyI GACNNNGTC 1 cut(s) 2236
PvuII CAGCTG 2 cut(s) 1427, 2222
RsaI GTAC 4 cut(s) 358, 1106, 1667, 2286
RsaNI GTAC 4 cut(s) 357, 1105, 1666, 2285
RseI CAYNNNNRTG 4 cut(s) 17, 783, 1201, 1818
SaqAI TTAA 8 cut(s) 6, 243, 660, 849, 921, 1271, 1397, 1566
SatI GCNGC 4 cut(s) 1258, 1425, 1428, 2220
Sau96I GGNCC 5 cut(s) 198, 877, 878, 970, 1478
SchI GAGTC 1 cut(s) 1359
ScrFI CCNGG 4 cut(s) 230, 480, 540, 1316
SduI GDGCHC 2 cut(s) 82, 881
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 5 cut(s) 1424, 2010, 2193, 2320, 2372
SfcI CTRYAG 2 cut(s) 2002, 2334
Sfr274I CTCGAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 707
SinI GGWCC 2 cut(s) 198, 1478
SlaI CTCGAG 1 cut(s) 130
SmiMI CAYNNNNRTG 4 cut(s) 17, 783, 1201, 1818
SmlI CTYRAG 2 cut(s) 130, 1767
SmoI CTYRAG 2 cut(s) 130, 1767
SpeI ACTAGT 1 cut(s) 68
SsiI CCGC 6 cut(s) 147, 624, 882, 1229, 1470, 1747
SspI AATATT 1 cut(s) 1793
SspMI CTAG 4 cut(s) 69, 1131, 1460, 2126
StyD4I CCNGG 4 cut(s) 228, 478, 538, 1314
StyI CCWWGG 2 cut(s) 678, 1054
TaaI ACNGT 6 cut(s) 193, 214, 839, 899, 1046, 1725
TaiI ACGT 4 cut(s) 167, 1028, 1106, 1945
TaqI TCGA 4 cut(s) 131, 707, 1062, 2394
TaqII GACCGA 1 cut(s) 2245
TatI WGTACW 1 cut(s) 1665
TfiI GAWTC 6 cut(s) 383, 569, 633, 674, 998, 2153
Tru1I TTAA 8 cut(s) 6, 243, 660, 849, 921, 1271, 1397, 1566
Tru9I TTAA 8 cut(s) 6, 243, 660, 849, 921, 1271, 1397, 1566
TscAI CASTG 3 cut(s) 1251, 1527, 2283
TseFI GTSAC 5 cut(s) 833, 1046, 1501, 2159, 2274
TseI GCWGC 4 cut(s) 1257, 1424, 1427, 2219
Tsp45I GTSAC 5 cut(s) 833, 1046, 1501, 2159, 2274
TspGWI ACGGA 2 cut(s) 333, 395
TspRI CASTG 3 cut(s) 1251, 1527, 2283
Tth111I GACNNNGTC 1 cut(s) 2236
Van91I CCANNNNNTGG 1 cut(s) 2147
VneI GTGCAC 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 198, 1478
VspI ATTAAT 2 cut(s) 660, 921
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 4 cut(s) 1336, 1496, 1866, 1978
XceI RCATGY 1 cut(s) 1672
XhoI CTCGAG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 1028
XspI CTAG 4 cut(s) 69, 1131, 1460, 2126
Zsp2I ATGCAT 2 cut(s) 1417, 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.