Rmu_sc0005564.1_g000005

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005564.1
OG0000096
Physical Location & Seq
Reverse (-)
17754 .. 20756
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005564.1_g000005.1.cds

Sequence Viewer

Length: 2421 bp
atgatcaacttattgtgcttgctcatcagtttctcaagcttctggactggtcatggtgcagctgcactgttcattggagacacgcttaatccaggggaggccctcaattcctcgagctttatggtctccgcttcgggggagtttacgttgggtttcaactctggctacctcattctgaggtgcaagaactatacggtttgggttgccaacctgcacactccgatttcagacccttcgtcggcgcttctcaccttggacaagaacagaactgtcctgcaaattaccctgaaaggcaagaatcccatcgttctttactccccttctcatgataatagtaccaataatgttgtggctaccctgttagattctggcaatttagtcctacgagaggtcaatcagaacgtttcaaagtccccgttgtggcaaagctttgatggtcctaatgtggctcttaggccggggatgaaattaggtttacgtgatggtctaaccggcaacactatgtcgatttggagttggcgggaaagtcaaaacagcccggggccagggtctttcagcctttattgggattccggcacacgccaattgcaagttaggagaaacggggatctttggtggactagcgaaacattgagaagtggaagatacgagtatgctaatttccgtaataaatccaaggtctacaattttagcgtggtttcatatgagaatggagactacttcagttacactgtcgttggtgatccaaatggcagatcagcctgggtgctagacacaggtgactgtgatggctataattatcacaccacctatagctctgtcgatggctataactccgttggtgggacgtgccagagatggagtggccgggccactctttattgtcctgtggcaacatcctctggtgcatttattcagccgactgaaaggcgctcctttagaccgatcagtatgaacatgtctatcaattcaacatgggaggagacaaattcaagtcgatatgggatttatgaatgtatggctacttgttggatggactgtgactgccttggcttcgaatttctctttgataatcagactggatgtcgattttggagtgtaaagtattctcaggtccttgaagatccggatagctctgcaactaaatatattataccaggactagtcgctacgtataaactgcaatctcgaagaaacataattgatgcctcatataagtggatatggattggtattgcaattgcggctgttcttctggcgctggtgctttgcagcatctgctacctaatacgaaagagaaaacttgcaggtgagaaccaaataaacatccaggatatgctgaacatcatgaaatctaatagaccaactgatgctaatgggcttcaaaatgatggaaagaggggacaagatttaagtgtatttagttatgcatctattatagctgccacttgcaacttctctaatgaaaataagctcggagaagggggttttggacctgtttataaggggaaattggtgactggacaagaagtagcagtgaaaaggctttcaaaacgttcagggcagggattatatgagtttaagaatgaattgatacttatatatgaactccagcatacaaaccttgttcagctgtttggattttgcattcatggtgaagagaggatgttgatatacgagtacatgccaaacaaaagcttggactactttttatttgattcaactagatgccagcagcagttagactggaacaaccgtttcagcataattgaaggaattgctcaaggtttactttacttgcacaaatattccagaatgaaagtaattcatagggatttgaaagcaagtaatgttctacttgatgcaagcatgaaccccaaaatttccgattttggaatggcaaggattttcacgaatgatgaacaagaagcaaatactatgaagattgttgggacacgtggatatatgtcccctgagtatattatggggggaaaattttccataaaatctgatgtctatagtttcggtgtattgatgcttgaaatcctaagtggtagaagaaacaacagcttctacaatgatgatcgcgccctcaatttagttggttatgcatgggcgttatggaaagaaggtgcaggactagaactaatggatccaacacttggcgattcatgtgataaagatcatttgttgaggtgcatccatgttggtttgctttgtgtagaggaaaatgcagccagtcgaccaaagatgttagatgtgatatctatgctgactaatgaaagcatgtcactgccagtacctacaaaaccagcattttgtacagaaagaaatgtcatcacaactactgtaggtggaaacggaccggaagtggtggtatcggtaaatggtatctccaattctgattatgatggacgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

806

Amino Acids

90.15

Weight (kDa)

6.8

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1503
AarI CACCTGC 1 cut(s) 1298
Acc36I ACCTGC 2 cut(s) 219, 1298
AccB7I CCANNNNNTGG 1 cut(s) 2162
AccI GTMKAC 2 cut(s) 681, 2242
AccII CGCG 1 cut(s) 2088
AccIII TCCGGA 1 cut(s) 1126
AciI CCGC 3 cut(s) 129, 520, 1244
AclI AACGTT 2 cut(s) 402, 1555
AclWI GGATC 5 cut(s) 615, 737, 1118, 2147, 2160
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 4 cut(s) 988, 1058, 1881, 1994
AcuI CTGAAG 1 cut(s) 706
AcvI CACGTG 1 cut(s) 1958
AfaI GTAC 4 cut(s) 337, 1682, 2301, 2323
AfiI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 545, 565, 843, 2162
AflIII ACRYGT 2 cut(s) 957, 1955
AhdI GACNNNNNGTC 2 cut(s) 235, 1083
AhlI ACTAGT 1 cut(s) 1162
AjiI CACGTC 1 cut(s) 849
AjnI CCWGG 5 cut(s) 91, 544, 761, 1156, 1329
AjuI GAANNNNNNNTTGG 2 cut(s) 1473, 1505
Alw26I GTCTC 4 cut(s) 72, 130, 708, 977
AlwI GGATC 5 cut(s) 615, 737, 1118, 2147, 2160
AlwNI CAGNNNCTG 1 cut(s) 1278
Ama87I CYCGRG 2 cut(s) 112, 538
Aor13HI TCCGGA 1 cut(s) 1126
AoxI GGCC 5 cut(s) 99, 455, 542, 865, 870
ApeKI GCWGC 6 cut(s) 59, 62, 1272, 1442, 1735, 2234
ApoI RAATTY 4 cut(s) 988, 1058, 1881, 1994
Asp700I GAANNNNTTC 1 cut(s) 1996
AspLEI GCGC 4 cut(s) 244, 933, 1261, 2090
AspS9I GGNCC 7 cut(s) 100, 437, 542, 870, 1114, 1493, 2363
AsuC2I CCSGG 4 cut(s) 459, 539, 540, 869
AsuHPI GGTGA 6 cut(s) 241, 752, 791, 1322, 1528, 1667
AsuII TTCGAA 1 cut(s) 1056
AvaI CYCGRG 2 cut(s) 112, 538
AvaII GGWCC 4 cut(s) 437, 1114, 1493, 2363
BamHI GGATCC 1 cut(s) 2152
BbrPI CACGTG 1 cut(s) 1958
BbvI GCAGC 6 cut(s) 49, 71, 1284, 1429, 1747, 2246
BccI CCATC 9 cut(s) 311, 428, 476, 782, 818, 852, 1027, 1385, 2405
BciT130I CCWGG 5 cut(s) 93, 546, 763, 1158, 1331
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 459, 539, 540, 869
BcoDI GTCTC 4 cut(s) 72, 130, 708, 977
BcuI ACTAGT 1 cut(s) 1162
BfaI CTAG 5 cut(s) 621, 770, 1163, 1725, 2141
BfmI CTRYAG 3 cut(s) 811, 2017, 2349
BfoI RGCGCY 3 cut(s) 245, 934, 1262
BfuAI ACCTGC 2 cut(s) 219, 1298
BisI GCNGC 7 cut(s) 60, 63, 1245, 1273, 1443, 1736, 2235
BlsI GCNGC 7 cut(s) 61, 64, 1246, 1274, 1444, 1737, 2236
Bme1390I CCNGG 9 cut(s) 93, 459, 539, 540, 546, 763, 869, 1158, 1331
Bme18I GGWCC 4 cut(s) 437, 1114, 1493, 2363
BmeRI GACNNNNNGTC 2 cut(s) 235, 1083
BmeT110I CYCGRG 2 cut(s) 112, 538
BmgBI CACGTC 1 cut(s) 849
BmgT120I GGNCC 7 cut(s) 100, 437, 542, 870, 1114, 1493, 2363
BmiI GGNNCC 2 cut(s) 543, 2154
BmrFI CCNGG 9 cut(s) 93, 459, 539, 540, 546, 763, 869, 1158, 1331
BmsI GCATC 8 cut(s) 1195, 1284, 1360, 1439, 1718, 1852, 2025, 2208
BpmI CTGGAG 1 cut(s) 1595
Bpu14I TTCGAA 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 19, 1767
BpuMI CCSGG 4 cut(s) 459, 539, 540, 869
BsaAI YACGTR 3 cut(s) 479, 1173, 1958
BsaBI GATNNNNATC 1 cut(s) 2181
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 9 cut(s) 92, 252, 458, 538, 539, 545, 675, 762, 1048
BsaWI WCCGGW 2 cut(s) 1126, 2365
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 545, 565, 843, 2162
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 6 cut(s) 52, 1084, 1525, 1751, 2238, 2297
Bse8I GATNNNNATC 1 cut(s) 2181
BseAI TCCGGA 1 cut(s) 1126
BseBI CCWGG 5 cut(s) 93, 546, 763, 1158, 1331
BseDI CCNNGG 9 cut(s) 92, 252, 458, 538, 539, 545, 675, 762, 1048
BseGI GGATG 7 cut(s) 468, 896, 1038, 1088, 1326, 1671, 2199
BseJI GATNNNNATC 1 cut(s) 2181
BseLI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 545, 565, 843, 2162
BseMII CTCAG 3 cut(s) 167, 1124, 1965
BseNI ACTGG 6 cut(s) 52, 1084, 1525, 1751, 2238, 2297
BseRI GAGGAG 1 cut(s) 995
BseXI GCAGC 6 cut(s) 49, 71, 1284, 1429, 1747, 2246
BsgI GTGCAG 4 cut(s) 48, 78, 197, 2154
Bsh1236I CGCG 1 cut(s) 2088
BshFI GGCC 5 cut(s) 101, 457, 544, 867, 872
BsiHKCI CYCGRG 2 cut(s) 112, 538
BsiSI CCGG 7 cut(s) 458, 492, 539, 573, 868, 1127, 2366
BslFI GGGAC 5 cut(s) 397, 859, 1416, 1954, 1966
BslI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 545, 565, 843, 2162
BsmAI GTCTC 4 cut(s) 72, 130, 708, 977
BsmFI GGGAC 5 cut(s) 397, 859, 1416, 1954, 1966
BsmI GAATGC 1 cut(s) 1647
BsnI GGCC 5 cut(s) 101, 457, 544, 867, 872
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 112, 538
Bsp119I TTCGAA 1 cut(s) 1056
Bsp13I TCCGGA 1 cut(s) 1126
Bsp1407I TGTACA 1 cut(s) 2321
Bsp143I GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
BspACI CCGC 3 cut(s) 129, 520, 1244
BspANI GGCC 5 cut(s) 101, 457, 544, 867, 872
BspCNI CTCAG 3 cut(s) 168, 1123, 1966
BspEI TCCGGA 1 cut(s) 1126
BspFNI CGCG 1 cut(s) 2088
BspHI TCATGA 2 cut(s) 325, 1347
BspLI GGNNCC 2 cut(s) 543, 2154
BspMI ACCTGC 2 cut(s) 219, 1298
BspPI GGATC 5 cut(s) 615, 737, 1118, 2147, 2160
BspT104I TTCGAA 1 cut(s) 1056
BspTNI GGTCTC 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 2321
BsrI ACTGG 6 cut(s) 52, 1084, 1525, 1751, 2238, 2297
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 9 cut(s) 92, 252, 458, 538, 539, 545, 675, 762, 1048
BssMI GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
BssT1I CCWWGG 3 cut(s) 252, 675, 1048
Bst2UI CCWGG 5 cut(s) 93, 546, 763, 1158, 1331
Bst4CI ACNGT 8 cut(s) 69, 196, 271, 733, 785, 1040, 1757, 2350
Bst6I CTCTTC 1 cut(s) 1653
BstAPI GCANNNNNTGC 1 cut(s) 1278
BstAUI TGTACA 1 cut(s) 2321
BstBAI YACGTR 3 cut(s) 479, 1173, 1958
BstBI TTCGAA 1 cut(s) 1056
BstC8I GCNNGC 3 cut(s) 20, 1733, 1867
BstDEI CTNAG 5 cut(s) 176, 452, 1110, 1974, 2048
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 7 cut(s) 468, 896, 1038, 1088, 1326, 1671, 2199
BstFNI CGCG 1 cut(s) 2088
BstH2I RGCGCY 3 cut(s) 245, 934, 1262
BstHHI GCGC 4 cut(s) 244, 933, 1261, 2090
BstKTI GATC 9 cut(s) 6, 610, 745, 758, 948, 1126, 2086, 2155, 2185
BstMAI GTCTC 4 cut(s) 72, 130, 708, 977
BstMBI GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
BstMWI GCNNNNNNNGC 2 cut(s) 1244, 1278
BstNI CCWGG 5 cut(s) 93, 546, 763, 1158, 1331
BstNSI RCATGY 3 cut(s) 961, 1687, 2290
BstSCI CCNGG 9 cut(s) 91, 457, 537, 538, 544, 761, 867, 1156, 1329
BstSFI CTRYAG 3 cut(s) 811, 2017, 2349
BstSNI TACGTA 1 cut(s) 1173
BstUI CGCG 1 cut(s) 2088
BstV1I GCAGC 6 cut(s) 49, 71, 1284, 1429, 1747, 2246
BstX2I RGATCY 3 cut(s) 607, 1123, 2152
BstYI RGATCY 3 cut(s) 607, 1123, 2152
BsuRI GGCC 5 cut(s) 101, 457, 544, 867, 872
BtrI CACGTC 1 cut(s) 849
BtsCI GGATG 7 cut(s) 468, 896, 1038, 1088, 1326, 1671, 2199
BtsI GCAGTG 2 cut(s) 1542, 2291
BtsIMutI CAGTG 4 cut(s) 65, 729, 1542, 2291
BveI ACCTGC 2 cut(s) 219, 1298
Cac8I GCNNGC 3 cut(s) 20, 1733, 1867
CaiI CAGNNNCTG 1 cut(s) 1278
CciI TCATGA 2 cut(s) 325, 1347
CfoI GCGC 4 cut(s) 244, 933, 1261, 2090
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 7 cut(s) 100, 437, 542, 870, 1114, 1493, 2363
Cfr9I CCCGGG 1 cut(s) 538
CpoI CGGWCCG 1 cut(s) 2363
Csp6I GTAC 4 cut(s) 336, 1681, 2300, 2322
CspI CGGWCCG 1 cut(s) 2363
CviQI GTAC 4 cut(s) 336, 1681, 2300, 2322
DdeI CTNAG 5 cut(s) 176, 452, 1110, 1974, 2048
DpnI GATC 9 cut(s) 5, 609, 744, 757, 947, 1125, 2085, 2154, 2184
DpnII GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
DriI GACNNNNNGTC 2 cut(s) 235, 1083
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 1 cut(s) 1653
Eam1105I GACNNNNNGTC 2 cut(s) 235, 1083
EarI CTCTTC 1 cut(s) 1653
Eco105I TACGTA 1 cut(s) 1173
Eco130I CCWWGG 3 cut(s) 252, 675, 1048
Eco31I GGTCTC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 2265
Eco47I GGWCC 4 cut(s) 437, 1114, 1493, 2363
Eco57I CTGAAG 1 cut(s) 706
Eco72I CACGTG 1 cut(s) 1958
Eco88I CYCGRG 2 cut(s) 112, 538
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 100, 1114
EcoRII CCWGG 5 cut(s) 91, 544, 761, 1156, 1329
EcoRV GATATC 1 cut(s) 2265
EcoT14I CCWWGG 3 cut(s) 252, 675, 1048
EcoT22I ATGCAT 2 cut(s) 1432, 2113
ErhI CCWWGG 3 cut(s) 252, 675, 1048
FalI AAGNNNNNCTT 2 cut(s) 1776, 1808
FaqI GGGAC 5 cut(s) 397, 859, 1416, 1954, 1966
FauI CCCGC 1 cut(s) 513
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 681, 2242
Fnu4HI GCNGC 7 cut(s) 60, 63, 1245, 1273, 1443, 1736, 2235
FokI GGATG 7 cut(s) 475, 883, 1045, 1095, 1313, 1678, 2186
Fsp4HI GCNGC 7 cut(s) 60, 63, 1245, 1273, 1443, 1736, 2235
FspBI CTAG 5 cut(s) 621, 770, 1163, 1725, 2141
GlaI GCGC 4 cut(s) 243, 932, 1260, 2089
GluI GCNGC 7 cut(s) 60, 63, 1245, 1273, 1443, 1736, 2235
GsuI CTGGAG 1 cut(s) 1595
HaeII RGCGCY 3 cut(s) 245, 934, 1262
HaeIII GGCC 5 cut(s) 101, 457, 544, 867, 872
HapII CCGG 7 cut(s) 458, 492, 539, 573, 868, 1127, 2366
HhaI GCGC 4 cut(s) 244, 933, 1261, 2090
Hin6I GCGC 4 cut(s) 242, 931, 1259, 2088
HinP1I GCGC 4 cut(s) 242, 931, 1259, 2088
HincII GTYRAC 1 cut(s) 2243
HindII GTYRAC 1 cut(s) 2243
HindIII AAGCTT 3 cut(s) 37, 427, 1696
HinfI GANTC 5 cut(s) 298, 365, 569, 1718, 2168
HpaII CCGG 7 cut(s) 458, 492, 539, 573, 868, 1127, 2366
HphI GGTGA 6 cut(s) 241, 752, 791, 1322, 1528, 1667
Hpy166II GTNNAC 6 cut(s) 144, 476, 618, 682, 1790, 2243
Hpy188I TCNGA 9 cut(s) 177, 222, 229, 399, 1077, 1478, 1888, 2011, 2404
Hpy188III TCNNGA 7 cut(s) 43, 326, 1127, 1188, 1348, 1812, 1912
Hpy8I GTNNAC 6 cut(s) 144, 476, 618, 682, 1790, 2243
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 243, 330, 1475, 1766, 2123
HpyCH4III ACNGT 8 cut(s) 69, 196, 271, 733, 785, 1040, 1757, 2350
HpyCH4IV ACGT 8 cut(s) 146, 402, 478, 848, 1172, 1555, 1957, 2416
HpyF10VI GCNNNNNNNGC 2 cut(s) 1244, 1278
HpyF3I CTNAG 5 cut(s) 176, 452, 1110, 1974, 2048
HpySE526I ACGT 8 cut(s) 146, 402, 478, 848, 1172, 1555, 1957, 2416
HspAI GCGC 4 cut(s) 242, 931, 1259, 2088
Kpn2I TCCGGA 1 cut(s) 1126
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
LmnI GCTCC 1 cut(s) 938
Lsp1109I GCAGC 6 cut(s) 49, 71, 1284, 1429, 1747, 2246
LweI GCATC 8 cut(s) 1195, 1284, 1360, 1439, 1718, 1852, 2025, 2208
MaeI CTAG 5 cut(s) 621, 770, 1163, 1725, 2141
MaeII ACGT 8 cut(s) 146, 402, 478, 848, 1172, 1555, 1957, 2416
MaeIII GTNAC 5 cut(s) 725, 779, 1040, 1516, 2289
MalI GATC 9 cut(s) 5, 609, 744, 757, 947, 1125, 2085, 2154, 2184
MboI GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
MboII GAAGA 7 cut(s) 654, 1133, 1203, 1244, 1670, 1954, 2070
MfeI CAATTG 2 cut(s) 584, 1239
MflI RGATCY 3 cut(s) 607, 1123, 2152
MmeI TCCRAC 2 cut(s) 1010, 2180
Mph1103I ATGCAT 2 cut(s) 1432, 2113
MroI TCCGGA 1 cut(s) 1126
MroXI GAANNNNTTC 1 cut(s) 1996
MseI TTAA 4 cut(s) 87, 1412, 1581, 2419
MslI CAYNNNNRTG 1 cut(s) 1216
MspA1I CMGCKG 2 cut(s) 62, 1633
MspI CCGG 7 cut(s) 458, 492, 539, 573, 868, 1127, 2366
MspR9I CCNGG 9 cut(s) 93, 459, 539, 540, 546, 763, 869, 1158, 1331
MunI CAATTG 2 cut(s) 584, 1239
Mva1269I GAATGC 1 cut(s) 1647
MvaI CCWGG 5 cut(s) 93, 546, 763, 1158, 1331
MvnI CGCG 1 cut(s) 2088
MwoI GCNNNNNNNGC 2 cut(s) 1244, 1278
NciI CCSGG 4 cut(s) 459, 539, 540, 869
NdeI CATATG 1 cut(s) 703
NdeII GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
NlaIV GGNNCC 2 cut(s) 543, 2154
NmuCI GTSAC 4 cut(s) 779, 1040, 1516, 2289
NsiI ATGCAT 2 cut(s) 1432, 2113
NspI RCATGY 3 cut(s) 961, 1687, 2290
NspV TTCGAA 1 cut(s) 1056
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 2 cut(s) 325, 1347
PaqCI CACCTGC 1 cut(s) 1298
PciI ACATGT 1 cut(s) 957
PctI GAATGC 1 cut(s) 1647
PdmI GAANNNNTTC 1 cut(s) 1996
PfeI GAWTC 5 cut(s) 298, 365, 569, 1718, 2168
PflMI CCANNNNNTGG 1 cut(s) 2162
PfoI TCCNGGA 1 cut(s) 1329
PkrI GCNGC 7 cut(s) 61, 64, 1246, 1274, 1444, 1737, 2236
PmaCI CACGTG 1 cut(s) 1958
PmlI CACGTG 1 cut(s) 1958
Ppu21I YACGTR 3 cut(s) 479, 1173, 1958
PpuMI RGGWCCY 1 cut(s) 1114
PscI ACATGT 1 cut(s) 957
PsiI TTATAA 1 cut(s) 1503
Psp1406I AACGTT 2 cut(s) 402, 1555
Psp5II RGGWCCY 1 cut(s) 1114
Psp6I CCWGG 5 cut(s) 91, 544, 761, 1156, 1329
PspCI CACGTG 1 cut(s) 1958
PspGI CCWGG 5 cut(s) 91, 544, 761, 1156, 1329
PspN4I GGNNCC 2 cut(s) 543, 2154
PspPI GGNCC 7 cut(s) 100, 437, 542, 870, 1114, 1493, 2363
PspPPI RGGWCCY 1 cut(s) 1114
PspXI VCTCGAGB 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 1278
PsuI RGATCY 3 cut(s) 607, 1123, 2152
PvuII CAGCTG 2 cut(s) 62, 1633
RsaI GTAC 4 cut(s) 337, 1682, 2301, 2323
RsaNI GTAC 4 cut(s) 336, 1681, 2300, 2322
RseI CAYNNNNRTG 1 cut(s) 1216
Rsr2I CGGWCCG 1 cut(s) 2363
RsrII CGGWCCG 1 cut(s) 2363
SalI GTCGAC 1 cut(s) 2241
SaqAI TTAA 4 cut(s) 87, 1412, 1581, 2419
SatI GCNGC 7 cut(s) 60, 63, 1245, 1273, 1443, 1736, 2235
Sau3AI GATC 9 cut(s) 3, 607, 742, 755, 945, 1123, 2083, 2152, 2182
Sau96I GGNCC 7 cut(s) 100, 437, 542, 870, 1114, 1493, 2363
ScrFI CCNGG 9 cut(s) 93, 459, 539, 540, 546, 763, 869, 1158, 1331
SfaNI GCATC 8 cut(s) 1195, 1284, 1360, 1439, 1718, 1852, 2025, 2208
SfcI CTRYAG 3 cut(s) 811, 2017, 2349
Sfr274I CTCGAG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 1056
SinI GGWCC 4 cut(s) 437, 1114, 1493, 2363
SlaI CTCGAG 1 cut(s) 112
SmaI CCCGGG 1 cut(s) 540
SmiMI CAYNNNNRTG 1 cut(s) 1216
SmlI CTYRAG 3 cut(s) 34, 112, 1782
SmoI CTYRAG 3 cut(s) 34, 112, 1782
SnaBI TACGTA 1 cut(s) 1173
SpeI ACTAGT 1 cut(s) 1162
SsiI CCGC 3 cut(s) 129, 520, 1244
SspI AATATT 1 cut(s) 1808
SspMI CTAG 5 cut(s) 621, 770, 1163, 1725, 2141
StyD4I CCNGG 9 cut(s) 91, 457, 537, 538, 544, 761, 867, 1156, 1329
StyI CCWWGG 3 cut(s) 252, 675, 1048
TaaI ACNGT 8 cut(s) 69, 196, 271, 733, 785, 1040, 1757, 2350
TaiI ACGT 8 cut(s) 149, 405, 481, 851, 1175, 1558, 1960, 2419
TaqI TCGA 8 cut(s) 113, 506, 822, 997, 1056, 1087, 1189, 2242
TaqII GACCGA 1 cut(s) 958
TatI WGTACW 2 cut(s) 1680, 2321
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 5 cut(s) 298, 365, 569, 1718, 2168
Tru1I TTAA 4 cut(s) 87, 1412, 1581, 2419
Tru9I TTAA 4 cut(s) 87, 1412, 1581, 2419
TscAI CASTG 4 cut(s) 72, 736, 1542, 2298
TseFI GTSAC 4 cut(s) 779, 1040, 1516, 2289
TseI GCWGC 6 cut(s) 59, 62, 1272, 1442, 1735, 2234
Tsp45I GTSAC 4 cut(s) 779, 1040, 1516, 2289
TspGWI ACGGA 3 cut(s) 653, 826, 2376
TspMI CCCGGG 1 cut(s) 538
TspRI CASTG 4 cut(s) 72, 736, 1542, 2298
Van91I CCANNNNNTGG 1 cut(s) 2162
VpaK11BI GGWCC 4 cut(s) 437, 1114, 1493, 2363
XagI CCTNNNNNAGG 1 cut(s) 386
XapI RAATTY 4 cut(s) 988, 1058, 1881, 1994
XceI RCATGY 3 cut(s) 961, 1687, 2290
XcmI CCANNNNNNNNNTGG 2 cut(s) 346, 860
XhoI CTCGAG 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 538
XmiI GTMKAC 2 cut(s) 681, 2242
XmnI GAANNNNTTC 1 cut(s) 1996
XspI CTAG 5 cut(s) 621, 770, 1163, 1725, 2141
Zsp2I ATGCAT 2 cut(s) 1432, 2113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.