RchiOBHm_Chr5g0025041

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000096
Physical Location & Seq
Forward (+)
19056953 .. 19060353
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30473

Sequence Viewer

Length: 2277 bp
ATGACTGATTTAGGGTTCATATTTCCAGTTCTGTCAACTGTTCTCAACTTGTTCTTCCTCATTTTTGCAATCTCGTGGACTTGTCATGAGGCTTTCTCGGACACACTCAAACCAGGGGACACTCTCAATTCCTCAAGTTTCTTAGTTTCTGCAAAGGGGAAGTTCACATTGGGTTTCCATGTAATTAATCCAAATTCCGAGTCCAGCTACCTAGCTATATGGCAGAACTACCTAAATACATGGCAGAAGAGAGGTAAAAGTCACGTATGGATTGCCAACAGAGACTCACCAATAGTATCCCCTTTGGGAGTTCTGACATTGGACGTCAATAAAACATTGAAAATTATGCACAAAGGTGGGGATCCTGTGGTGCTTTACCCTGCTTCTGAAACTACCAGTATTTACAGTACTAGTAGTGTTGTGGCTACTCTATTGGATTCTGGAAATTTTATCATGCAAGAACTGAACTCTGACGGATCAGTGAAGCATGTACTGTGGCAAAGTTTTGATTATCCTACACACACCCTTTTGCCCGGCATGAAAGTAGGAGTTAACCATATAAATGGCCACATTTGGTCGATTTCATCATGGTCAAGTGTGTATTGGCCAGCACCAGGGCCTTTCACCCTTGTTTGGGACCCTAATGGACGTGAACTGAAAATCAAGCGACGAGGGGTGGTTTATTGGACGAGTGGAATCTTTAGAGATGGGCAATTTGAATTTATGAAACCTTATGATGATATGCGTATGATCAGGTTTGAGTTTAGCATTGTTTCAAATGAGAATGAAGACTACTTCACTTACACTACTGCTAGTGTTAATCAAAGTCATAAACCAGAATGGGTTCTGAACATATTTGGGCAACTCCTTGAATTAGAAGGAAATGTTATTGCACCAGCAGATCAGTGTTATGGCTTTAACACTGATAAAGGGTGCCAGAGTCGGGACTATCCCAGTTGCAGGCATATTGGTGACACATTTGTTGAGAAAAATGGTTATTTAAAATCACTCACATCCAATCAGACAATTAAGCTTGACTTAAATACAAGTCTCACCCTCAGCGATTGTAAGGAAGCTTGCAGGGAAGATTGTGACTGTCTTGGATTCCTGTCTCTATTCGATAATCAGACTGGATGCAAATTTTGGGTTGGAAACTGGGTCTTTAGTTCACATGACCTCTTTGGTTATAGTAATCCAAGAATTTTTATCCTATCTGAGTTATCGCGCAATGGGAAACGCCAGCAAAAGATTGAGAAGGAATTGCTTGATTTGGTGCAATCTGATAAATCTTTTGATGTCAAGGGGCTTCCAACTGATGAAAGGGTGGGACATTGTGATTTGGGAGTTTATACTTATGCATCTGTCTTGGGAAAGTCAGTGACAGGAAGAGACATAGCTGTGAAAAGACTTTCCAGATGTTCAGGGCAAGGAGCATTGGAGTTTAAGAATGAATTGATACTAATATCTGAACTCCAACATACGAACCTTGTTCAACTTTTGGGATTTTGCACTCATGGGGAAGAGAGGATGTTGATATACGAGTATATGCCAAACAGAAGCTTGGACTACTTTTTGTTTGATTCAACCAGAGTGATGCTACTAGATTGGAATAAGCGTTGCAGTATAATAGAAGGAATCGCTCAAGGATTGCTTTATTTGCACAAATACTCGAGAGTGAGAGTAATTCATAGAGATTTGAAAGCTAGTAACATACTACTTGATGAAAACATGATTCCCAGAATTTCCGACTTTGGGATGGCAAGGATTTTCTCGCACAATGAACCACAAGCAAATACTAACAGGGTTGTTGGCACATATGGTTACATGTCTCCTGAGTATGCCATGCGTGGAACTTTCTCCACAAAATCTGATATCTACAGTTTTGGTGTAATGATGCTTGAAATCATAAGTGGCAGGAAAAACAACAGCTTCCACAGCGATGATCCAGTGCTCAATATAGTAGGATATGCATGGGAGTTGTGGAAGGATGGTCGTGGGCTAGAATTAATGGATCCAACACTATCCGATTCTTGTATTGAGGATCAATTTTTAAGATGCATCCATGTCGGTCTGTTATGTGTCGAAGAAAATGCAGTTGATCGGCCCACCATATCGGATATCATATCTATGCTGACAAATGAAAGTATTTCTCTACCTGTACCTACAAAGCCAGCATTTTGTATAGAAAGAAAAATTGTCAGGGCTGGTATAGATGGAGACGGATCAGAAATTATATTAGTAAATGATATGTCCAATTCTGAATTTGAGGCACGTTAA

Protein Analysis

758

Amino Acids

85.81

Weight (kDa)

5.68

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 197 2.4e-24 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 440 - 654 2.1e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 459 - 643 3.1e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 933
AccII CGCG 1 cut(s) 1225
AclWI GGATC 8 cut(s) 356, 369, 484, 1937, 2006, 2019, 2049, 2230
AcoI YGGCCR 2 cut(s) 565, 605
AcsI RAATTY 7 cut(s) 193, 445, 719, 1139, 1200, 1740, 2261
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 3 cut(s) 409, 492, 2160
AfiI CCNNNNNNNGG 6 cut(s) 614, 633, 634, 943, 960, 1752
AflIII ACRYGT 1 cut(s) 1824
AgsI TTSAA 8 cut(s) 340, 719, 777, 872, 1493, 1584, 1699, 1901
AhlI ACTAGT 1 cut(s) 410
AjiI CACGTC 1 cut(s) 650
AjnI CCWGG 2 cut(s) 112, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 8 cut(s) 207, 215, 1033, 1076, 1397, 1560, 1703, 1929
AluI AGCT 8 cut(s) 207, 215, 1033, 1076, 1397, 1560, 1703, 1929
Alw21I GWGCWC 1 cut(s) 1953
Alw26I GTCTC 6 cut(s) 276, 1055, 1116, 1383, 1833, 2211
AlwI GGATC 8 cut(s) 356, 369, 484, 1937, 2006, 2019, 2049, 2230
Ama87I CYCGRG 1 cut(s) 1669
AoxI GGCC 4 cut(s) 565, 605, 617, 2102
ApoI RAATTY 7 cut(s) 193, 445, 719, 1139, 1200, 1740, 2261
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 2 cut(s) 186, 2006
Asp700I GAANNNNTTC 1 cut(s) 843
AspLEI GCGC 1 cut(s) 1227
AspS9I GGNCC 3 cut(s) 617, 637, 2103
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 4 cut(s) 279, 616, 983, 1045
AvaI CYCGRG 1 cut(s) 1669
AvaII GGWCC 1 cut(s) 637
BalI TGGCCA 2 cut(s) 567, 607
BamHI GGATCC 2 cut(s) 361, 2011
BanI GGYRCC 1 cut(s) 933
BauI CACGAG 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 1953
BbvCI CCTCAGC 1 cut(s) 1058
BccI CCATC 4 cut(s) 701, 1750, 1982, 2207
BcgI CGANNNNNNTGC 4 cut(s) 2047, 2072, 2081, 2106
BciT130I CCWGG 2 cut(s) 114, 615
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 1 cut(s) 750
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 6 cut(s) 276, 1055, 1116, 1383, 1833, 2211
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 6 cut(s) 212, 411, 813, 1601, 1704, 2000
BfmI CTRYAG 1 cut(s) 1876
BfuI GTATCC 1 cut(s) 307
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 3 cut(s) 114, 534, 615
Bme18I GGWCC 1 cut(s) 637
BmeT110I CYCGRG 1 cut(s) 1669
BmgBI CACGTC 1 cut(s) 650
BmgT120I GGNCC 3 cut(s) 617, 637, 2103
BmiI GGNNCC 5 cut(s) 363, 638, 639, 935, 2013
BmrFI CCNGG 3 cut(s) 114, 534, 615
BmrI ACTGGG 2 cut(s) 948, 1165
BmsI GCATC 6 cut(s) 1124, 1367, 1584, 1884, 2045, 2067
BmuI ACTGGG 2 cut(s) 948, 1165
BpiI GAAGAC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 80, 112
Bpu10I CCTNAGC 1 cut(s) 1058
BpuEI CTTGAG 2 cut(s) 118, 1626
BpuMI CCSGG 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 113, 614
Bsc4I CCNNNNNNNGG 6 cut(s) 614, 633, 634, 943, 960, 1752
Bse1I ACTGG 6 cut(s) 26, 396, 954, 1135, 1160, 1946
Bse3DI GCAATG 1 cut(s) 1234
BseBI CCWGG 2 cut(s) 114, 615
BseDI CCNNGG 2 cut(s) 113, 614
BseGI GGATG 6 cut(s) 1013, 1139, 1533, 1761, 1993, 2058
BseLI CCNNNNNNNGG 6 cut(s) 614, 633, 634, 943, 960, 1752
BseMI GCAATG 1 cut(s) 1234
BseMII CTCAG 3 cut(s) 1072, 1206, 1824
BseNI ACTGG 6 cut(s) 26, 396, 954, 1135, 1160, 1946
Bsh1236I CGCG 1 cut(s) 1225
BshFI GGCC 4 cut(s) 567, 607, 619, 2104
BshNI GGYRCC 1 cut(s) 933
BsiHKAI GWGCWC 1 cut(s) 1953
BsiHKCI CYCGRG 1 cut(s) 1669
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 4 cut(s) 131, 650, 959, 1341
BslI CCNNNNNNNGG 6 cut(s) 614, 633, 634, 943, 960, 1752
BsmAI GTCTC 6 cut(s) 276, 1055, 1116, 1383, 1833, 2211
BsmBI CGTCTC 1 cut(s) 2211
BsmFI GGGAC 4 cut(s) 131, 650, 959, 1341
BsnI GGCC 4 cut(s) 567, 607, 619, 2104
BsoBI CYCGRG 1 cut(s) 1669
Bsp1286I GDGCHC 1 cut(s) 1953
Bsp143I GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
BspANI GGCC 4 cut(s) 567, 607, 619, 2104
BspCNI CTCAG 3 cut(s) 1071, 1207, 1825
BspFNI CGCG 1 cut(s) 1225
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 5 cut(s) 363, 638, 639, 935, 2013
BspPI GGATC 8 cut(s) 356, 369, 484, 1937, 2006, 2019, 2049, 2230
BspT107I GGYRCC 1 cut(s) 933
BsrDI GCAATG 1 cut(s) 1234
BsrI ACTGG 6 cut(s) 26, 396, 954, 1135, 1160, 1946
BssECI CCNNGG 2 cut(s) 113, 614
BssMI GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 114, 615
Bst4CI ACNGT 5 cut(s) 40, 407, 495, 1097, 1880
Bst6I CTCTTC 3 cut(s) 242, 1381, 1515
BstACI GRCGYC 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 5 cut(s) 609, 962, 1078, 1241, 2172
BstDEI CTNAG 4 cut(s) 142, 1058, 1215, 1833
BstF5I GGATG 6 cut(s) 1013, 1139, 1533, 1761, 1993, 2058
BstFNI CGCG 1 cut(s) 1225
BstHHI GCGC 1 cut(s) 1227
BstKTI GATC 9 cut(s) 364, 479, 753, 904, 1945, 2014, 2044, 2101, 2225
BstMAI GTCTC 6 cut(s) 276, 1055, 1116, 1383, 1833, 2211
BstMBI GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
BstMWI GCNNNNNNNGC 2 cut(s) 1657, 1935
BstNI CCWGG 2 cut(s) 114, 615
BstNSI RCATGY 2 cut(s) 491, 1828
BstSCI CCNGG 3 cut(s) 112, 532, 613
BstSFI CTRYAG 1 cut(s) 1876
BstUI CGCG 1 cut(s) 1225
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 2 cut(s) 361, 2011
BstXI CCANNNNNNTGG 1 cut(s) 563
BstYI RGATCY 2 cut(s) 361, 2011
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 4 cut(s) 567, 607, 619, 2104
BtgZI GCGATG 1 cut(s) 1953
BtrI CACGTC 1 cut(s) 650
BtsCI GGATG 6 cut(s) 1013, 1139, 1533, 1761, 1993, 2058
BtsIMutI CAGTG 5 cut(s) 486, 911, 921, 1383, 1953
Cac8I GCNNGC 5 cut(s) 609, 962, 1078, 1241, 2172
CciI TCATGA 1 cut(s) 85
CfoI GCGC 1 cut(s) 1227
Cfr13I GGNCC 3 cut(s) 617, 637, 2103
Csp6I GTAC 3 cut(s) 408, 491, 2159
CviQI GTAC 3 cut(s) 408, 491, 2159
DdeI CTNAG 4 cut(s) 142, 1058, 1215, 1833
DpnI GATC 9 cut(s) 363, 478, 752, 903, 1944, 2013, 2043, 2100, 2224
DpnII GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
DraI TTTAAA 1 cut(s) 1002
EaeI YGGCCR 2 cut(s) 565, 605
Eam1104I CTCTTC 3 cut(s) 242, 1381, 1515
EarI CTCTTC 3 cut(s) 242, 1381, 1515
Eco32I GATATC 2 cut(s) 1873, 2119
Eco47I GGWCC 1 cut(s) 637
Eco88I CYCGRG 1 cut(s) 1669
EcoO109I RGGNCCY 2 cut(s) 617, 637
EcoRII CCWGG 2 cut(s) 112, 613
EcoRV GATATC 2 cut(s) 1873, 2119
EcoT22I ATGCAT 3 cut(s) 1360, 1972, 2060
Esp3I CGTCTC 1 cut(s) 2211
FalI AAGNNNNNCTT 4 cut(s) 1022, 1054, 1635, 1667
FaqI GGGAC 4 cut(s) 131, 650, 959, 1341
FauNDI CATATG 1 cut(s) 1816
FbaI TGATCA 1 cut(s) 750
FokI GGATG 6 cut(s) 1000, 1146, 1540, 1768, 2000, 2045
FspBI CTAG 6 cut(s) 212, 411, 813, 1601, 1704, 2000
GlaI GCGC 1 cut(s) 1226
HaeIII GGCC 4 cut(s) 567, 607, 619, 2104
HapII CCGG 1 cut(s) 534
HhaI GCGC 1 cut(s) 1227
Hin1I GRCGYC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 1225
HinP1I GCGC 1 cut(s) 1225
HincII GTYRAC 2 cut(s) 36, 553
HindII GTYRAC 2 cut(s) 36, 553
HindIII AAGCTT 3 cut(s) 1031, 1074, 1558
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 1 cut(s) 534
HphI GGTGA 4 cut(s) 279, 616, 983, 1045
Hpy166II GTNNAC 6 cut(s) 36, 78, 165, 553, 653, 1169
Hpy188III TCNNGA 6 cut(s) 86, 441, 944, 1413, 1671, 1832
Hpy8I GTNNAC 6 cut(s) 36, 78, 165, 553, 653, 1169
Hpy99I CGWCG 1 cut(s) 672
HpyAV CCTTC 4 cut(s) 872, 1249, 1625, 1978
HpyCH4III ACNGT 5 cut(s) 40, 407, 495, 1097, 1880
HpyCH4IV ACGT 4 cut(s) 264, 324, 649, 2272
HpyF10VI GCNNNNNNNGC 2 cut(s) 1657, 1935
HpyF3I CTNAG 4 cut(s) 142, 1058, 1215, 1833
HpySE526I ACGT 4 cut(s) 264, 324, 649, 2272
Hsp92I GRCGYC 1 cut(s) 324
HspAI GCGC 1 cut(s) 1225
KflI GGGWCCC 1 cut(s) 637
Ksp22I TGATCA 1 cut(s) 750
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
LmnI GCTCC 1 cut(s) 1430
LweI GCATC 6 cut(s) 1124, 1367, 1584, 1884, 2045, 2067
MaeI CTAG 6 cut(s) 212, 411, 813, 1601, 1704, 2000
MaeII ACGT 4 cut(s) 264, 324, 649, 2272
MaeIII GTNAC 6 cut(s) 260, 971, 1091, 1378, 1706, 1820
MalI GATC 9 cut(s) 363, 478, 752, 903, 1944, 2013, 2043, 2100, 2224
MboI GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
MboII GAAGA 7 cut(s) 46, 259, 800, 1097, 1398, 1532, 2096
MflI RGATCY 2 cut(s) 361, 2011
MhlI GDGCHC 1 cut(s) 1953
MlsI TGGCCA 2 cut(s) 567, 607
MluNI TGGCCA 2 cut(s) 567, 607
MlyI GAGTC 3 cut(s) 209, 278, 949
MmeI TCCRAC 5 cut(s) 1129, 1334, 1498, 1770, 2039
Mox20I TGGCCA 2 cut(s) 567, 607
Mph1103I ATGCAT 3 cut(s) 1360, 1972, 2060
MroXI GAANNNNTTC 1 cut(s) 843
MscI TGGCCA 2 cut(s) 567, 607
MslI CAYNNNNRTG 6 cut(s) 354, 561, 969, 1397, 1938, 2126
Msp20I TGGCCA 2 cut(s) 567, 607
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 3 cut(s) 114, 534, 615
MvaI CCWGG 2 cut(s) 114, 615
MvnI CGCG 1 cut(s) 1225
MwoI GCNNNNNNNGC 2 cut(s) 1657, 1935
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 1816
NdeII GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
NlaIV GGNNCC 5 cut(s) 363, 638, 639, 935, 2013
NmuCI GTSAC 4 cut(s) 260, 971, 1091, 1378
NsiI ATGCAT 3 cut(s) 1360, 1972, 2060
NspI RCATGY 2 cut(s) 491, 1828
OliI CACNNNNGTG 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 1669
PagI TCATGA 1 cut(s) 85
PciI ACATGT 1 cut(s) 1824
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 7 cut(s) 437, 696, 1104, 1580, 1635, 1732, 2027
PleI GAGTC 3 cut(s) 208, 278, 948
PpsI GAGTC 3 cut(s) 208, 278, 948
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 637
PscI ACATGT 1 cut(s) 1824
PshBI ATTAAT 2 cut(s) 186, 2006
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 2 cut(s) 112, 613
PspGI CCWGG 2 cut(s) 112, 613
PspN4I GGNNCC 5 cut(s) 363, 638, 639, 935, 2013
PspPI GGNCC 3 cut(s) 617, 637, 2103
PspPPI RGGWCCY 1 cut(s) 637
PsuI RGATCY 2 cut(s) 361, 2011
RsaI GTAC 3 cut(s) 409, 492, 2160
RsaNI GTAC 3 cut(s) 408, 491, 2159
RseI CAYNNNNRTG 6 cut(s) 354, 561, 969, 1397, 1938, 2126
Sau3AI GATC 9 cut(s) 361, 476, 750, 901, 1942, 2011, 2041, 2098, 2222
Sau96I GGNCC 3 cut(s) 617, 637, 2103
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 3 cut(s) 209, 278, 949
ScrFI CCNGG 3 cut(s) 114, 534, 615
SduI GDGCHC 1 cut(s) 1953
SfaNI GCATC 6 cut(s) 1124, 1367, 1584, 1884, 2045, 2067
SfcI CTRYAG 1 cut(s) 1876
Sfr274I CTCGAG 1 cut(s) 1669
SinI GGWCC 1 cut(s) 637
SlaI CTCGAG 1 cut(s) 1669
SmiMI CAYNNNNRTG 6 cut(s) 354, 561, 969, 1397, 1938, 2126
SmlI CTYRAG 3 cut(s) 133, 1641, 1669
SmoI CTYRAG 3 cut(s) 133, 1641, 1669
SpeI ACTAGT 1 cut(s) 410
SspMI CTAG 6 cut(s) 212, 411, 813, 1601, 1704, 2000
StyD4I CCNGG 3 cut(s) 112, 532, 613
TaaI ACNGT 5 cut(s) 40, 407, 495, 1097, 1880
TaiI ACGT 4 cut(s) 267, 327, 652, 2275
TaqI TCGA 4 cut(s) 578, 1119, 1670, 2082
TaqII GACCGA 1 cut(s) 2057
TatI WGTACW 2 cut(s) 407, 490
TfiI GAWTC 7 cut(s) 437, 696, 1104, 1580, 1635, 1732, 2027
TscAI CASTG 5 cut(s) 486, 911, 928, 1383, 1953
TseFI GTSAC 4 cut(s) 260, 971, 1091, 1378
Tsp45I GTSAC 4 cut(s) 260, 971, 1091, 1378
TspGWI ACGGA 2 cut(s) 489, 2235
TspRI CASTG 5 cut(s) 486, 911, 928, 1383, 1953
VpaK11BI GGWCC 1 cut(s) 637
VspI ATTAAT 2 cut(s) 186, 2006
XapI RAATTY 7 cut(s) 193, 445, 719, 1139, 1200, 1740, 2261
XceI RCATGY 2 cut(s) 491, 1828
XhoI CTCGAG 1 cut(s) 1669
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 6 cut(s) 212, 411, 813, 1601, 1704, 2000
ZraI GACGTC 1 cut(s) 325
ZrmI AGTACT 1 cut(s) 409
Zsp2I ATGCAT 3 cut(s) 1360, 1972, 2060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.