RchiOBHm_Chr5g0024871

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000096
Physical Location & Seq
Forward (+)
18897170 .. 18901416
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30458

Sequence Viewer

Length: 2310 bp
ATGAACACACTGGAACCAGGCGACACTCTCGATTCTTCTAGCTTCTTAGTTTCTGCAAATGGGAAGTTCACCCTGAATTTTCGTAAATATGATGATGCTTTAAGCTTGAGCTACCTGGTTATAAAGTGGAATGGAAGTCATAATTATGCCTGGGTTGCAAATCCGTACACACCTGTTTTATACCCTTTTGGAGTTCTTACGTTGGACAGGAACAACACATTGAAAATCACTCAGAAAGATGGTGATCCAGTGGTGCTTTACTCAGCTTCAGAGACCGCCAATAGCAGTGTGTTAGCTACCCTTTTGGATTCTGGCAACTTTGTCCTAGAAGAGGTGAGCTCTGATGGATCAGTGAAAAGGGTTTTGTGGCAAAGTTTTGATTATCCAGGAGATGTGCTTCTGCCAGGTATGAAATTAGGGATTAATCATAGTAATGGCCACAACTGGTCACTTTCATCGTGGTTTACTGAGAAAAGTGCAATGCCAGGATCTTTCACACTTGATTGGGACCCTGAGGGACATGAATTGAAAATTAGGCGACGGGGGGTAGTTTATTGGAGTAGTGGAATCTTTAGAGATGGGAGTTTTGAAAATATTAAGCAGAAGAGGTATACTTTTAGCATTGTTTCAAATCAGAATGAAGATTATTTCAGTTACTCTGTTGTAGATCAAAATGCTAAACCACAATGGCTCCTAACCACAATAGGGAGACTATATGACTTTGATGGATCACTGGATATTGCAACAGCGGATTCCTGTTATGGGTATAACACTGATGGAGGATGCCAGATTTGGGACCAACCAACTGAGTGCAGGCATTTTGGTGATGTATTTGAGCAAGGAAATGGGTACTTTAATCCGACAGGTCCTAGTGGTTCAAATGTAAAATCTACCAGTGATTCAAACACAAGTCTCAGCATAACTGATTGTAAGGCTGCTTGTTGGGAAAATTGTGACTGTCACGGGTTCATGTTTCTGTTTGCTAATCAGACTGGATGTCGATTTTGGACTGGAAACTTGAAGTTCATTGCAGACAGCGCAGGCTATAGTTCAAGTGTTGTTTATTTTTTAACAAAAAAGTCAGCCAGCAGCCGCTCACATAAGTGGATATGGATTGGTGTTGCTACTGGCACGGCTCTATTGGTAATGGTGCTTTGCATAGTATGCTGTCTATTACGAAGAAGAAACTTGTCCGGAGGGGAGAACAAAAAAAAGATTGATGAGAAAAAATTGCTTCCCTTAAGGGGATCTGATATATCAACTGATGTCCATGGGATTCAAAATGATGGATCCATGGGACATGATTTAAGAGCATTTAGCTATGAATCTGTCATGACTGCTACAGACTACCTCTCCTTACAAAACAAGCTAGGAGAAGGGGGCTTTGGACCTGTTTATAAGGGAAAATTGTCGTCAGGGCGAGAAGTAGCTGTAAAGAGGCTTTCAAGTTGTTCCGGGCAAGGAGAAGTACAGTTTAAGAATGAATTGATTCTCATATCTGAACTCCAACATACTAATCTTGTTCAGCTTTTCGGATTTTGCATTCATGGTGAAGAGAGGATGCTAATATACGAGTATATGCCAAACAAAGATCTGGACTACATATTATTTGATTCAACCAGAAGCATGCTACTGGATTGGAAGAAACGTTTCAATATAATTGAAGGAATCGCCCAAGGATTGCTTTACTTGCACAAGTACTCTAGATTAAAAGTAATTCATAGAGATCTTAAAGCTAGTAACATACTACTAGATGAAAATATGAACCCCAAAATTTCTGATTTTGGAACGGCAAGGAGTTTCATGCTTAATGAAGTTGAAGCAAGTACTGGACGGATTGTTGGGACACATGGTTACATGTCTCCCGAGTATGCTATGCAGGGAATTTTTTCTGGAAAATCTGATGTCTTCAGTTTTGGAGTGTTGATGCTCGAAATCATAAGTGGTAGGAAAAACAACAGGTTTCACAGTGCTCATCGCGCGCTCAACTTAGTAGGATATGCGTGGGAATTATGGCAAGAAGGTGCAGGACTAGAACTGATGGACACAACACTAGGTGATTCGTGTGTGGAAAATCAATTTTTAAGATGCACCCATGTTGGTCTACTATGTGTCGAAGAAGATGCAGAACTTCGGCCTACCATGTCAGATGCCATATCTATGTTGACAAATGAAAGCTTGCCTTTACCTATACCAATAAGACCAGTATGTTTTCCAGTAAGGCATTCGGCTGGAGTTGACATAAGAGGAAACAAATCCGAAATTATATCAGCAAATGGCCTGTCCAGCACCCTTGTTATAGGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

769

Amino Acids

85.71

Weight (kDa)

5.95

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 50 - 154 2e-24 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 452 - 669 1.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 453 - 652 3.5e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 122, 1398
AccBSI CCGCTC 1 cut(s) 1095
AccI GTMKAC 2 cut(s) 611, 2104
AccII CGCG 2 cut(s) 1980, 1982
AccIII TCCGGA 1 cut(s) 1193
AciI CCGC 3 cut(s) 276, 749, 1093
AclI AACGTT 1 cut(s) 1648
AclWI GGATC 7 cut(s) 239, 355, 496, 736, 1255, 1284, 1297
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 76, 1773, 1884
AcuI CTGAAG 2 cut(s) 252, 1894
AdeI CACNNNGTG 1 cut(s) 2057
AfaI GTAC 5 cut(s) 167, 851, 1470, 1700, 1828
AfiI CCNNNNNNNGG 5 cut(s) 331, 705, 762, 793, 1244
AflII CTTAAG 1 cut(s) 1240
AflIII ACRYGT 1 cut(s) 1857
AhdI GACNNNNNGTC 1 cut(s) 996
AjnI CCWGG 6 cut(s) 16, 114, 149, 385, 403, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 1368, 1400
AleI CACNNNNGTG 1 cut(s) 1102
Alw21I GWGCWC 2 cut(s) 341, 1975
Alw26I GTCTC 4 cut(s) 266, 703, 917, 1866
AlwI GGATC 7 cut(s) 239, 355, 496, 736, 1255, 1284, 1297
Ama87I CYCGRG 1 cut(s) 1865
Aor13HI TCCGGA 1 cut(s) 1193
AoxI GGCC 3 cut(s) 436, 2135, 2278
ApeKI GCWGC 2 cut(s) 935, 1089
ApoI RAATTY 3 cut(s) 76, 1773, 1884
ArsI GACNNNNNNTTYG 2 cut(s) 2267, 2299
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 3 cut(s) 1488, 1649, 1888
AspLEI GCGC 3 cut(s) 1040, 1982, 1984
AspS9I GGNCC 4 cut(s) 508, 796, 866, 1388
AsuC2I CCSGG 1 cut(s) 1456
AsuHPI GGTGA 6 cut(s) 61, 254, 346, 836, 1562, 2069
AvaI CYCGRG 1 cut(s) 1865
AvaII GGWCC 4 cut(s) 508, 796, 866, 1388
AxyI CCTNAGG 1 cut(s) 513
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 1289
BanII GRGCYC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 1900
Bbv12I GWGCWC 2 cut(s) 341, 1975
BbvI GCAGC 2 cut(s) 922, 1101
BccI CCATC 7 cut(s) 233, 338, 572, 719, 770, 1280, 2035
BceAI ACGGC 2 cut(s) 1149, 1806
BcgI CGANNNNNNTGC 2 cut(s) 2105, 2139
BciT130I CCWGG 6 cut(s) 18, 116, 151, 387, 405, 486
BcnI CCSGG 1 cut(s) 1456
BcoDI GTCTC 4 cut(s) 266, 703, 917, 1866
BfaI CTAG 9 cut(s) 39, 326, 870, 1370, 1704, 1737, 1751, 2033, 2054
BfmI CTRYAG 2 cut(s) 1045, 1341
BfrI CTTAAG 1 cut(s) 1240
BglII AGATCT 2 cut(s) 1591, 1726
BisI GCNGC 3 cut(s) 936, 1090, 1093
BlsI GCNGC 3 cut(s) 937, 1091, 1094
BmcAI AGTACT 2 cut(s) 1700, 1828
Bme1390I CCNGG 7 cut(s) 18, 116, 151, 387, 405, 486, 1456
Bme18I GGWCC 4 cut(s) 508, 796, 866, 1388
BmeRI GACNNNNNGTC 1 cut(s) 996
BmeT110I CYCGRG 1 cut(s) 1865
BmgT120I GGNCC 4 cut(s) 508, 796, 866, 1388
BmiI GGNNCC 6 cut(s) 15, 509, 510, 692, 797, 1291
BmrFI CCNGG 7 cut(s) 18, 116, 151, 387, 405, 486, 1456
BmsI GCATC 7 cut(s) 85, 773, 1551, 1917, 2078, 2113, 2140
BpiI GAAGAC 1 cut(s) 1900
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 1 cut(s) 2253
BpuEI CTTGAG 1 cut(s) 127
BpuMI CCSGG 1 cut(s) 1456
BsaBI GATNNNNATC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 150, 1270, 1293, 1675
BsaWI WCCGGW 1 cut(s) 1193
BsaXI ACNNNNNCTCC 4 cut(s) 675, 705, 1337, 1367
Bsc4I CCNNNNNNNGG 5 cut(s) 331, 705, 762, 793, 1244
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 2 cut(s) 486, 1026
Bse8I GATNNNNATC 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 1193
BseBI CCWGG 6 cut(s) 18, 116, 151, 387, 405, 486
BseDI CCNNGG 4 cut(s) 150, 1270, 1293, 1675
BseGI GGATG 3 cut(s) 788, 1001, 1566
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 5 cut(s) 331, 705, 762, 793, 1244
BseMI GCAATG 2 cut(s) 486, 1026
BseMII CTCAG 6 cut(s) 245, 276, 459, 504, 798, 928
BsePI GCGCGC 1 cut(s) 1980
BseXI GCAGC 2 cut(s) 922, 1101
BsgI GTGCAG 2 cut(s) 832, 2046
Bsh1236I CGCG 2 cut(s) 1980, 1982
BshFI GGCC 3 cut(s) 438, 2137, 2280
BsiHKAI GWGCWC 2 cut(s) 341, 1975
BsiHKCI CYCGRG 1 cut(s) 1865
BsiSI CCGG 2 cut(s) 1194, 1455
BslFI GGGAC 5 cut(s) 521, 531, 809, 1311, 1858
BslI CCNNNNNNNGG 5 cut(s) 331, 705, 762, 793, 1244
BsmAI GTCTC 4 cut(s) 266, 703, 917, 1866
BsmFI GGGAC 5 cut(s) 521, 531, 809, 1311, 1858
BsmI GAATGC 2 cut(s) 1542, 2224
BsnI GGCC 3 cut(s) 438, 2137, 2280
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 1865
Bsp1286I GDGCHC 2 cut(s) 341, 1975
Bsp13I TCCGGA 1 cut(s) 1193
Bsp143I GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
Bsp19I CCATGG 2 cut(s) 1270, 1293
BspACI CCGC 3 cut(s) 276, 749, 1093
BspANI GGCC 3 cut(s) 438, 2137, 2280
BspCNI CTCAG 6 cut(s) 244, 275, 460, 505, 799, 927
BspEI TCCGGA 1 cut(s) 1193
BspFNI CGCG 2 cut(s) 1980, 1982
BspHI TCATGA 1 cut(s) 1332
BspLI GGNNCC 6 cut(s) 15, 509, 510, 692, 797, 1291
BspPI GGATC 7 cut(s) 239, 355, 496, 736, 1255, 1284, 1297
BspTI CTTAAG 1 cut(s) 1240
BspTNI GGTCTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 1095
BsrDI GCAATG 2 cut(s) 486, 1026
BssECI CCNNGG 4 cut(s) 150, 1270, 1293, 1675
BssHII GCGCGC 1 cut(s) 1980
BssMI GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
BssNAI GTATAC 1 cut(s) 612
BssT1I CCWWGG 3 cut(s) 1270, 1293, 1675
Bst1107I GTATAC 1 cut(s) 612
Bst2UI CCWGG 6 cut(s) 18, 116, 151, 387, 405, 486
Bst4CI ACNGT 3 cut(s) 959, 1473, 1970
Bst6I CTCTTC 3 cut(s) 324, 599, 1548
BstAFI CTTAAG 1 cut(s) 1240
BstAPI GCANNNNNTGC 1 cut(s) 1164
BstC8I GCNNGC 6 cut(s) 815, 1042, 1087, 1628, 1982, 2180
BstDEI CTNAG 8 cut(s) 46, 231, 262, 468, 513, 807, 914, 1990
BstDSI CCRYGG 2 cut(s) 1270, 1293
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 3 cut(s) 788, 1001, 1566
BstFNI CGCG 2 cut(s) 1980, 1982
BstHHI GCGC 3 cut(s) 1040, 1982, 1984
BstKTI GATC 9 cut(s) 247, 350, 491, 670, 731, 1250, 1292, 1594, 1729
BstMAI GTCTC 4 cut(s) 266, 703, 917, 1866
BstMBI GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
BstMWI GCNNNNNNNGC 6 cut(s) 155, 1037, 1164, 1690, 1979, 2286
BstNI CCWGG 6 cut(s) 18, 116, 151, 387, 405, 486
BstNSI RCATGY 2 cut(s) 1630, 1861
BstSCI CCNGG 7 cut(s) 16, 114, 149, 385, 403, 484, 1454
BstSFI CTRYAG 2 cut(s) 1045, 1341
BstUI CGCG 2 cut(s) 1980, 1982
BstV1I GCAGC 2 cut(s) 922, 1101
BstV2I GAAGAC 1 cut(s) 1900
BstX2I RGATCY 5 cut(s) 488, 1247, 1289, 1591, 1726
BstYI RGATCY 5 cut(s) 488, 1247, 1289, 1591, 1726
BstZ17I GTATAC 1 cut(s) 612
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 3 cut(s) 438, 2137, 2280
BtgI CCRYGG 2 cut(s) 1270, 1293
BtgZI GCGATG 1 cut(s) 1961
BtsCI GGATG 3 cut(s) 788, 1001, 1566
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 8 cut(s) 8, 255, 292, 357, 731, 771, 901, 1975
Cac8I GCNNGC 6 cut(s) 815, 1042, 1087, 1628, 1982, 2180
CciI TCATGA 1 cut(s) 1332
CfoI GCGC 3 cut(s) 1040, 1982, 1984
Cfr13I GGNCC 4 cut(s) 508, 796, 866, 1388
CsiI ACCWGGT 1 cut(s) 114
Csp6I GTAC 5 cut(s) 166, 850, 1469, 1699, 1827
CviQI GTAC 5 cut(s) 166, 850, 1469, 1699, 1827
DdeI CTNAG 8 cut(s) 46, 231, 262, 468, 513, 807, 914, 1990
DpnI GATC 9 cut(s) 246, 349, 490, 669, 730, 1249, 1291, 1593, 1728
DpnII GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
DraIII CACNNNGTG 1 cut(s) 2057
DriI GACNNNNNGTC 1 cut(s) 996
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 3 cut(s) 324, 599, 1548
Eam1105I GACNNNNNGTC 1 cut(s) 996
EarI CTCTTC 3 cut(s) 324, 599, 1548
Ecl136II GAGCTC 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 1270, 1293, 1675
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 4 cut(s) 508, 796, 866, 1388
Eco53kI GAGCTC 1 cut(s) 339
Eco57I CTGAAG 2 cut(s) 252, 1894
Eco81I CCTNAGG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 1865
EcoICRI GAGCTC 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 508, 866
EcoRII CCWGG 6 cut(s) 16, 114, 149, 385, 403, 484
EcoT14I CCWWGG 3 cut(s) 1270, 1293, 1675
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 3 cut(s) 1270, 1293, 1675
FalI AAGNNNNNCTT 4 cut(s) 1668, 1700, 2167, 2199
FaqI GGGAC 5 cut(s) 521, 531, 809, 1311, 1858
FblI GTMKAC 2 cut(s) 611, 2104
Fnu4HI GCNGC 3 cut(s) 936, 1090, 1093
FokI GGATG 3 cut(s) 795, 1008, 1573
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 3 cut(s) 936, 1090, 1093
FspBI CTAG 9 cut(s) 39, 326, 870, 1370, 1704, 1737, 1751, 2033, 2054
GlaI GCGC 3 cut(s) 1039, 1981, 1983
GluI GCNGC 3 cut(s) 936, 1090, 1093
GsuI CTGGAG 1 cut(s) 2253
HaeIII GGCC 3 cut(s) 438, 2137, 2280
HapII CCGG 2 cut(s) 1194, 1455
HhaI GCGC 3 cut(s) 1040, 1982, 1984
Hin6I GCGC 3 cut(s) 1038, 1980, 1982
HinP1I GCGC 3 cut(s) 1038, 1980, 1982
HincII GTYRAC 2 cut(s) 2166, 2239
HindII GTYRAC 2 cut(s) 2166, 2239
HindIII AAGCTT 2 cut(s) 103, 2176
HpaII CCGG 2 cut(s) 1194, 1455
HphI GGTGA 6 cut(s) 61, 254, 346, 836, 1562, 2069
Hpy166II GTNNAC 7 cut(s) 69, 168, 465, 612, 2105, 2166, 2239
Hpy188III TCNNGA 7 cut(s) 29, 1194, 1333, 1595, 1704, 1865, 1893
Hpy8I GTNNAC 7 cut(s) 69, 168, 465, 612, 2105, 2166, 2239
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 3 cut(s) 1370, 1658, 2015
HpyCH4III ACNGT 3 cut(s) 959, 1473, 1970
HpyCH4IV ACGT 2 cut(s) 200, 1648
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 1037, 1164, 1690, 1979, 2286
HpyF3I CTNAG 8 cut(s) 46, 231, 262, 468, 513, 807, 914, 1990
HpySE526I ACGT 2 cut(s) 200, 1648
HspAI GCGC 3 cut(s) 1038, 1980, 1982
KflI GGGWCCC 1 cut(s) 508
Kpn2I TCCGGA 1 cut(s) 1193
Kzo9I GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
LmnI GCTCC 1 cut(s) 696
Lsp1109I GCAGC 2 cut(s) 922, 1101
LweI GCATC 7 cut(s) 85, 773, 1551, 1917, 2078, 2113, 2140
MabI ACCWGGT 1 cut(s) 114
MaeI CTAG 9 cut(s) 39, 326, 870, 1370, 1704, 1737, 1751, 2033, 2054
MaeII ACGT 2 cut(s) 200, 1648
MaeIII GTNAC 6 cut(s) 447, 653, 953, 959, 1739, 1853
MalI GATC 9 cut(s) 246, 349, 490, 669, 730, 1249, 1291, 1593, 1728
MbiI CCGCTC 1 cut(s) 1095
MboI GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
MflI RGATCY 5 cut(s) 488, 1247, 1289, 1591, 1726
MhlI GDGCHC 2 cut(s) 341, 1975
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MmeI TCCRAC 3 cut(s) 183, 884, 1531
MnlI CCTC 9 cut(s) 325, 508, 600, 773, 1190, 1361, 1431, 1551, 2240
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 1 cut(s) 1193
MroXI GAANNNNTTC 3 cut(s) 1488, 1649, 1888
MscI TGGCCA 1 cut(s) 438
MslI CAYNNNNRTG 5 cut(s) 144, 432, 822, 1102, 2159
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 1240
MspI CCGG 2 cut(s) 1194, 1455
MspR9I CCNGG 7 cut(s) 18, 116, 151, 387, 405, 486, 1456
Mva1269I GAATGC 2 cut(s) 1542, 2224
MvaI CCWGG 6 cut(s) 18, 116, 151, 387, 405, 486
MvnI CGCG 2 cut(s) 1980, 1982
MwoI GCNNNNNNNGC 6 cut(s) 155, 1037, 1164, 1690, 1979, 2286
NciI CCSGG 1 cut(s) 1456
NcoI CCATGG 2 cut(s) 1270, 1293
NdeII GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
NlaIV GGNNCC 6 cut(s) 15, 509, 510, 692, 797, 1291
NmuCI GTSAC 3 cut(s) 447, 953, 959
NspI RCATGY 2 cut(s) 1630, 1861
OliI CACNNNNGTG 1 cut(s) 1102
PaeI GCATGC 1 cut(s) 1630
PagI TCATGA 1 cut(s) 1332
PauI GCGCGC 1 cut(s) 1980
PciI ACATGT 1 cut(s) 1857
PctI GAATGC 2 cut(s) 1542, 2224
PdmI GAANNNNTTC 3 cut(s) 1488, 1649, 1888
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 3 cut(s) 937, 1091, 1094
PpuMI RGGWCCY 2 cut(s) 508, 866
PscI ACATGT 1 cut(s) 1857
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 2 cut(s) 122, 1398
Psp124BI GAGCTC 1 cut(s) 341
Psp1406I AACGTT 1 cut(s) 1648
Psp5II RGGWCCY 2 cut(s) 508, 866
Psp6I CCWGG 6 cut(s) 16, 114, 149, 385, 403, 484
PspGI CCWGG 6 cut(s) 16, 114, 149, 385, 403, 484
PspN4I GGNNCC 6 cut(s) 15, 509, 510, 692, 797, 1291
PspPI GGNCC 4 cut(s) 508, 796, 866, 1388
PspPPI RGGWCCY 2 cut(s) 508, 866
PsuI RGATCY 5 cut(s) 488, 1247, 1289, 1591, 1726
PteI GCGCGC 1 cut(s) 1980
RsaI GTAC 5 cut(s) 167, 851, 1470, 1700, 1828
RsaNI GTAC 5 cut(s) 166, 850, 1469, 1699, 1827
RseI CAYNNNNRTG 5 cut(s) 144, 432, 822, 1102, 2159
SacI GAGCTC 1 cut(s) 341
SatI GCNGC 3 cut(s) 936, 1090, 1093
Sau3AI GATC 9 cut(s) 244, 347, 488, 667, 728, 1247, 1289, 1591, 1726
Sau96I GGNCC 4 cut(s) 508, 796, 866, 1388
ScaI AGTACT 2 cut(s) 1700, 1828
ScrFI CCNGG 7 cut(s) 18, 116, 151, 387, 405, 486, 1456
SduI GDGCHC 2 cut(s) 341, 1975
SexAI ACCWGGT 1 cut(s) 114
SfaNI GCATC 7 cut(s) 85, 773, 1551, 1917, 2078, 2113, 2140
SfcI CTRYAG 2 cut(s) 1045, 1341
SinI GGWCC 4 cut(s) 508, 796, 866, 1388
SmiMI CAYNNNNRTG 5 cut(s) 144, 432, 822, 1102, 2159
SmlI CTYRAG 2 cut(s) 106, 1240
SmoI CTYRAG 2 cut(s) 106, 1240
SphI GCATGC 1 cut(s) 1630
SsiI CCGC 3 cut(s) 276, 749, 1093
SspI AATATT 1 cut(s) 595
SspMI CTAG 9 cut(s) 39, 326, 870, 1370, 1704, 1737, 1751, 2033, 2054
SstI GAGCTC 1 cut(s) 341
StyD4I CCNGG 7 cut(s) 16, 114, 149, 385, 403, 484, 1454
StyI CCWWGG 3 cut(s) 1270, 1293, 1675
TaaI ACNGT 3 cut(s) 959, 1473, 1970
TaiI ACGT 2 cut(s) 203, 1651
TaqI TCGA 4 cut(s) 30, 1000, 1932, 2115
TatI WGTACW 3 cut(s) 1468, 1698, 1826
TauI GCSGC 1 cut(s) 1095
TscAI CASTG 8 cut(s) 15, 255, 292, 357, 738, 778, 901, 1975
TseFI GTSAC 3 cut(s) 447, 953, 959
TseI GCWGC 2 cut(s) 935, 1089
Tsp45I GTSAC 3 cut(s) 447, 953, 959
TspGWI ACGGA 2 cut(s) 153, 1849
TspRI CASTG 8 cut(s) 15, 255, 292, 357, 738, 778, 901, 1975
Vha464I CTTAAG 1 cut(s) 1240
VpaK11BI GGWCC 4 cut(s) 508, 796, 866, 1388
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 3 cut(s) 76, 1773, 1884
XbaI TCTAGA 1 cut(s) 1703
XceI RCATGY 2 cut(s) 1630, 1861
XmiI GTMKAC 2 cut(s) 611, 2104
XmnI GAANNNNTTC 3 cut(s) 1488, 1649, 1888
XspI CTAG 9 cut(s) 39, 326, 870, 1370, 1704, 1737, 1751, 2033, 2054
ZrmI AGTACT 2 cut(s) 1700, 1828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.