RchiOBHm_Chr5g0024971

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000096
Physical Location & Seq
Forward (+)
19002027 .. 19008265
6239 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30466

Sequence Viewer

Length: 3243 bp
ATGAGAGACAATTTCTCTGCAGAAAACAAGCTCGGAGAAGGGGGCTTTGGACCTGTTTATAAGGTTCGGTACAAGCTTGTTCAGCTTTTTGGATATTGCATTTATGGTGAAGAGAGGATGCTGATATACGAGTATATGCCAAACAAAAGTCTGGACTACATTTCATTTGATTCTAGCAGATGCATGCTACAAGATTGGAAGAAGCGTTTCAATATAATCGAAGGAATCGCTCAAGGATTGCTTTACTTGCACAAACACTCAAGATCAAAAGTAATTCATAGAGATCTTAAAGCTAGTAACATACTACTTGATGAAAATATGTACCCCAAAATTTCTGATTTTGGTACGGCAAGGATGTTCACACGGCATGAAGTTCAAGCAATTACTAGACGAGTTGTTGGGACATTTTTTGGAGTGTTGATGCTTGAAATCATAAGTGGTAGGAAAAACAACAGCTTCCACACTGCTCATCGTGCGCCCAATTTAGTAGGATATGCATGGGAGTTATGGCAAGAAGATGCAGGACTAGAATTGATGGACGCAACTCTAACTGATTCGTGTGTTGAAAATCAATTTTTAAGATGCATCCATGTTGGTCTGCCATGCGTCGAAGAAGATGCAGAACTTCGGCCAACCATGTCAGATGCTATATCTATGCTGACAAATGAAAGCTTTTGCCTTTACCTATACCAACAAGACCAGCATCTTTTCCAGTGGGTGAATTGGGTTGAAGCACAAAAAAGGGTCTCTAGAATATTTTGCTTTTTTGGCTGTACAGTACAGCGCAGCAAGAAAAATTTTCAGCATAGATTTTATCATTTGGATTTTGGAGGAGTACTCTGTGGTTTTGGTGATTTAGCATTTATGGCTGGCGGTGTCGATTTGATTTGCTTAGTGTTTATGTGGAGCTTGTGGAGTATTTGTGATGGTGCAACAAACACACTGAAACCAGGTGAAAGTCTAAATTCTTCAAGCTTGTTAGTTTCTGCAAATGGGAAGTTCACTTTGAATTTTCGTGTTTATGAGCATGAGGCAAACTTGAGTTACTTAGTTATAAAGTGGAATGCGAGTCATAACTATGCGTGGGTTGCAAACAGAGAAACACCCATTTTGTACCCTTTTGGAGTTCTTACATTGGACAGGAACTACACATTGAAGATTACTGACAGAGATGGGGATGCTGTGGTGCTTTACTCTGCTGATTCGGAGACTATCAGTGGTGATGCTGTGGCTACTCTTATGGATGATGGGAATTTTGTGCTGCAAGAAGTGAGTGATGATGGATCGGTGAAGAGGGATTTGTGGCAGGGTTTTGATTATCCTGGGGATGTGCTTCTGCCAGGTATGAAATTAGGGGTTAACCGTAGTAATGGGCATAATTGGTCACTTTCGTGCTGGTTAACTGAGAAAAGTGCAGTGCCAGGACCTTTCACTCTTGAATGGGACCCTGATGGACACGAATTGAAAATTAAGCGGCGTGGGGTGGTGTATTGGAGTAGTGGAGTGTTTCGAGATGGGAATTTTGAAAATATTAAGCAGAAGAGGTTTAGTTTTAGCGTTGTTTCAAAGAAGAATGAAGACTATTTCAGTTACACTGCTTTAGATGAAAATGCTGTATCAGAATGGATGCTAACCACAATAGGGCGACTACAAGACTTTGATGAATCAATTGATATTGCAAAAGCAGATTCCTGTTATGGGTATAACACAGATGGAGGATGCCAGATTTGGGACCAGCCAACTAAGTGTAGGCGTTATGGTGATGTATTTGAGCAAGAAAATGGTTACTTCAATCCAACGGATTCTAGTGGCTCTACTGCAACATCAACAAGTGATCCAAATACAAGTCTCACTATTAGTGATTGTAAGGCTGCTTGTTGGGCAGATTGTAACTGTCGTGGATTCATCTTTCTGTTTGTTAATCAGACTGGATGCCGGTATTGGACTGGAAACTTGAAATTCATTGCAGACAGCGCAGGTTATAGTTCAAGTGTTGTATATTTTTTAACAACAAAGTCAGACAGCAGCTCGCATAAGTGGATATGGATTGGCGCTACTATTGGCACTTCTGTATTTTTAATGGCGGTTTGCATCATCTGCTGTCTATTAAGAAGAAGAAAACTCTCCCTTTTTGGGAAGAACAAAGCAAAGATTGATGAGAAGGACCTGCTTGACTTAAGGGGATCTGATATATCAACTGATGTCCACGGGATTCAGAATGATGGATCGATGGGACATGATTTAAGAGCATTTAGCTATGAATCTATCATGGCTGCCACAGACTGCTTCTCCTTACAAAACAAGCTAGGAGAGGGGGGCTTTGGACCTGTTTATAAGGGAAAATTGTCAGAGGGGCGAGAAGTAGCTATAAAGAGGCTTTCAAGAGGTTCGGTGCAGGGAACATTGGAGTTTAAAAATGAATTGATACTCATATCTGAACTCCAACACACTAACCTTGTTCAGCTCTTGGGATATTGCATTCATGGTGTAGAGAGGATGTTAATATACGAGTATCTGTCAAACAAAAGTTTAGACTACATCTTATTTGATTCAACCAGAGGCATGCTACTAGATTGGAAGAAGCGTTTCAATATAATTGAAGGAATTGCTCAAGGATTGATTTACTTGCACAAATACTCAAGATTGAAAGTAATCCATAGAGATCTTAAAGCTAGTAACATACTACTTGATGAAGATATGAATCCCAAAATTTCTGATTTTGGTTTGGCAAGGATTTTCACGGTTAATGAAGTTGAAGCAAATACTAATCGGATTGTTGGCACATATGGTTACATGTCTCCTGAGTATGCTATGGAGGGAATTTTTTCTGGCAAATCTGATGTCTTTAGTTTTGGAGTGTTGATGCTTGAAATCATAAGTGGTAGGAGAAACAACAGCTTCCACAATGCTCATCGCTCGCTCAATTTAGTAGGATATACATGGGAGTTATGGAAAGAAGGTGCAGGACTAGATTTAATGGATCCAAGGCTAAGCGATTCATGTAATAGAGATCAACTGCTAAGATGCATACATGTTGGTCTATTATGCGTGGAAGAAGATGCAGAACATCGGCCAACCATGTCAGATGCCATATCACTGTTGACAAATGAAAGCTTGCCATTACCTACACCAACAAAACCAGCATTTTTCCCAGTAAGACGTGTGGCTGAAGTTGATGGAAGTGGAAACAGATCAGAAATTTCGTCAAATGACTTGTCAAACTCCTTTGTTATTGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

1080

Amino Acids

122.24

Weight (kDa)

5.83

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 25 - 124 4.8e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 25 - 135 4.1e-18 Protein kinase domain
B_lectin PF01453 361 - 466 4.8e-23 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 764 - 1028 9.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 764 - 970 1.2e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 60, 1055, 2334
Acc36I ACCTGC 2 cut(s) 1967, 2175
AciI CCGC 3 cut(s) 873, 1474, 2084
AclWI GGATC 6 cut(s) 1291, 1829, 2191, 2233, 2975, 2988
AcoI YGGCCR 2 cut(s) 629, 3071
AcuI CTGAAG 1 cut(s) 3189
AfaI GTAC 7 cut(s) 71, 323, 346, 775, 780, 837, 1115
AfiI CCNNNNNNNGG 4 cut(s) 1641, 1698, 1729, 2133
AflII CTTAAG 1 cut(s) 2176
AflIII ACRYGT 3 cut(s) 2793, 3031, 3160
AjiI CACGTC 1 cut(s) 3161
AjnI CCWGG 4 cut(s) 949, 1321, 1339, 1420
AjuI GAANNNNNNNTTGG 2 cut(s) 30, 62
AleI CACNNNNGTG 1 cut(s) 1390
Alw26I GTCTC 4 cut(s) 751, 1202, 1853, 2802
AlwI GGATC 6 cut(s) 1291, 1829, 2191, 2233, 2975, 2988
AoxI GGCC 2 cut(s) 629, 3071
ApeKI GCWGC 5 cut(s) 786, 1261, 1871, 2025, 2273
ArsI GACNNNNNNTTYG 4 cut(s) 2826, 2858, 3200, 3232
Asp700I GAANNNNTTC 3 cut(s) 206, 2585, 2824
AspLEI GCGC 4 cut(s) 478, 786, 1976, 2054
AspS9I GGNCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
AsuHPI GGTGA 7 cut(s) 119, 730, 863, 965, 1232, 1300, 1772
AvaII GGWCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
BamHI GGATCC 1 cut(s) 2980
BbsI GAAGAC 1 cut(s) 1584
BbvI GCAGC 5 cut(s) 798, 1248, 1858, 2037, 2260
BceAI ACGGC 2 cut(s) 363, 380
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 4 cut(s) 951, 1323, 1341, 1422
BcoDI GTCTC 4 cut(s) 751, 1202, 1853, 2802
BfmI CTRYAG 1 cut(s) 18
BfoI RGCGCY 1 cut(s) 2055
BfrI CTTAAG 1 cut(s) 2176
BfuAI ACCTGC 2 cut(s) 1967, 2175
BglII AGATCT 2 cut(s) 283, 2662
BisI GCNGC 6 cut(s) 787, 1262, 1475, 1872, 2026, 2274
BlpI GCTNAGC 1 cut(s) 2990
BlsI GCNGC 6 cut(s) 788, 1263, 1476, 1873, 2027, 2275
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 4 cut(s) 951, 1323, 1341, 1422
Bme18I GGWCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
BmgBI CACGTC 1 cut(s) 3161
BmgT120I GGNCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
BmiI GGNNCC 4 cut(s) 1445, 1446, 1733, 2982
BmrFI CCNGG 4 cut(s) 951, 1323, 1341, 1422
BmrI ACTGGG 1 cut(s) 3146
BmuI ACTGGG 1 cut(s) 3146
BpiI GAAGAC 1 cut(s) 1584
BplI GAGNNNNNCTC 2 cut(s) 822, 854
Bpu1102I GCTNAGC 1 cut(s) 2990
BpuEI CTTGAG 5 cut(s) 216, 244, 1060, 2595, 2623
Bsa29I ATCGAT 1 cut(s) 2228
BsaBI GATNNNNATC 1 cut(s) 2700
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 3 cut(s) 1322, 2206, 2984
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 2273, 2303
Bsc4I CCNNNNNNNGG 4 cut(s) 1641, 1698, 1729, 2133
Bse118I RCCGGY 1 cut(s) 1935
Bse1I ACTGG 4 cut(s) 712, 1933, 1951, 3152
Bse3DI GCAATG 1 cut(s) 1962
Bse8I GATNNNNATC 1 cut(s) 2700
BseBI CCWGG 4 cut(s) 951, 1323, 1341, 1422
BseCI ATCGAT 1 cut(s) 2228
BseDI CCNNGG 3 cut(s) 1322, 2206, 2984
BseJI GATNNNNATC 1 cut(s) 2700
BseLI CCNNNNNNNGG 4 cut(s) 1641, 1698, 1729, 2133
BseMI GCAATG 1 cut(s) 1962
BseMII CTCAG 2 cut(s) 1395, 2793
BseNI ACTGG 4 cut(s) 712, 1933, 1951, 3152
BseRI GAGGAG 1 cut(s) 846
BseXI GCAGC 5 cut(s) 798, 1248, 1858, 2037, 2260
BsgI GTGCAG 3 cut(s) 1434, 2414, 2982
BshFI GGCC 2 cut(s) 631, 3073
BshVI ATCGAT 1 cut(s) 2228
BsiSI CCGG 1 cut(s) 1936
BslFI GGGAC 4 cut(s) 415, 1457, 1745, 2247
BslI CCNNNNNNNGG 4 cut(s) 1641, 1698, 1729, 2133
BsmAI GTCTC 4 cut(s) 751, 1202, 1853, 2802
BsmFI GGGAC 4 cut(s) 415, 1457, 1745, 2247
BsmI GAATGC 2 cut(s) 1069, 2478
BsnI GGCC 2 cut(s) 631, 3073
Bso31I GGTCTC 1 cut(s) 751
Bsp1407I TGTACA 1 cut(s) 773
Bsp1720I GCTNAGC 1 cut(s) 2990
BspACI CCGC 3 cut(s) 873, 1474, 2084
BspANI GGCC 2 cut(s) 631, 3073
BspCNI CTCAG 2 cut(s) 1396, 2794
BspDI ATCGAT 1 cut(s) 2228
BspLI GGNNCC 4 cut(s) 1445, 1446, 1733, 2982
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 2 cut(s) 1967, 2175
BspPI GGATC 6 cut(s) 1291, 1829, 2191, 2233, 2975, 2988
BspTI CTTAAG 1 cut(s) 2176
BspTNI GGTCTC 1 cut(s) 751
BsrDI GCAATG 1 cut(s) 1962
BsrFI RCCGGY 1 cut(s) 1935
BsrGI TGTACA 1 cut(s) 773
BsrI ACTGG 4 cut(s) 712, 1933, 1951, 3152
BssAI RCCGGY 1 cut(s) 1935
BssECI CCNNGG 3 cut(s) 1322, 2206, 2984
BssT1I CCWWGG 1 cut(s) 2984
Bst2UI CCWGG 4 cut(s) 951, 1323, 1341, 1422
Bst4CI ACNGT 5 cut(s) 778, 1364, 1895, 2743, 3099
Bst6I CTCTTC 3 cut(s) 105, 1286, 1535
BstAFI CTTAAG 1 cut(s) 2176
BstAPI GCANNNNNTGC 1 cut(s) 2097
BstAUI TGTACA 1 cut(s) 773
BstC8I GCNNGC 6 cut(s) 185, 871, 2030, 2564, 2918, 3116
BstDEI CTNAG 7 cut(s) 892, 1048, 1404, 1743, 2802, 2990, 3020
BstDSI CCRYGG 1 cut(s) 2206
BstH2I RGCGCY 1 cut(s) 2055
BstHHI GCGC 4 cut(s) 478, 786, 1976, 2054
BstMAI GTCTC 4 cut(s) 751, 1202, 1853, 2802
BstMWI GCNNNNNNNGC 9 cut(s) 82, 247, 473, 768, 866, 1088, 1880, 1973, 2097
BstNI CCWGG 4 cut(s) 951, 1323, 1341, 1422
BstNSI RCATGY 4 cut(s) 187, 2566, 2797, 3035
BstSCI CCNGG 4 cut(s) 949, 1321, 1339, 1420
BstSFI CTRYAG 1 cut(s) 18
BstV1I GCAGC 5 cut(s) 798, 1248, 1858, 2037, 2260
BstV2I GAAGAC 1 cut(s) 1584
BstX2I RGATCY 4 cut(s) 283, 2183, 2662, 2980
BstYI RGATCY 4 cut(s) 283, 2183, 2662, 2980
Bsu15I ATCGAT 1 cut(s) 2228
BsuRI GGCC 2 cut(s) 631, 3073
BsuTUI ATCGAT 1 cut(s) 2228
BtgI CCRYGG 1 cut(s) 2206
BtgZI GCGATG 1 cut(s) 2897
BtrI CACGTC 1 cut(s) 3161
BtsI GCAGTG 3 cut(s) 462, 1422, 1593
BtsIMutI CAGTG 7 cut(s) 462, 719, 941, 1222, 1422, 1593, 3095
BveI ACCTGC 2 cut(s) 1967, 2175
Cac8I GCNNGC 6 cut(s) 185, 871, 2030, 2564, 2918, 3116
CfoI GCGC 4 cut(s) 478, 786, 1976, 2054
Cfr10I RCCGGY 1 cut(s) 1935
Cfr13I GGNCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
ClaI ATCGAT 1 cut(s) 2228
CseI GACGC 2 cut(s) 548, 595
CsiI ACCWGGT 1 cut(s) 949
Csp6I GTAC 7 cut(s) 70, 322, 345, 774, 779, 836, 1114
CviQI GTAC 7 cut(s) 70, 322, 345, 774, 779, 836, 1114
DdeI CTNAG 7 cut(s) 892, 1048, 1404, 1743, 2802, 2990, 3020
DraI TTTAAA 1 cut(s) 2413
EaeI YGGCCR 2 cut(s) 629, 3071
Eam1104I CTCTTC 3 cut(s) 105, 1286, 1535
EarI CTCTTC 3 cut(s) 105, 1286, 1535
Eco130I CCWWGG 1 cut(s) 2984
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
Eco57I CTGAAG 1 cut(s) 3189
EcoO109I RGGNCCY 3 cut(s) 1424, 1444, 2164
EcoRII CCWGG 4 cut(s) 949, 1321, 1339, 1420
EcoT14I CCWWGG 1 cut(s) 2984
EcoT22I ATGCAT 4 cut(s) 185, 499, 587, 3029
ErhI CCWWGG 1 cut(s) 2984
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 4 cut(s) 415, 1457, 1745, 2247
FauNDI CATATG 1 cut(s) 2785
Fnu4HI GCNGC 6 cut(s) 787, 1262, 1475, 1872, 2026, 2274
Fsp4HI GCNGC 6 cut(s) 787, 1262, 1475, 1872, 2026, 2274
GlaI GCGC 4 cut(s) 477, 785, 1975, 2053
GluI GCNGC 6 cut(s) 787, 1262, 1475, 1872, 2026, 2274
HaeII RGCGCY 1 cut(s) 2055
HaeIII GGCC 2 cut(s) 631, 3073
HapII CCGG 1 cut(s) 1936
HgaI GACGC 2 cut(s) 548, 595
HhaI GCGC 4 cut(s) 478, 786, 1976, 2054
Hin6I GCGC 4 cut(s) 476, 784, 1974, 2052
HinP1I GCGC 4 cut(s) 476, 784, 1974, 2052
HincII GTYRAC 3 cut(s) 1360, 1401, 3102
HindII GTYRAC 3 cut(s) 1360, 1401, 3102
HindIII AAGCTT 4 cut(s) 74, 670, 973, 3112
HpaI GTTAAC 2 cut(s) 1360, 1401
HpaII CCGG 1 cut(s) 1936
HphI GGTGA 7 cut(s) 119, 730, 863, 965, 1232, 1300, 1772
Hpy166II GTNNAC 6 cut(s) 360, 1002, 1360, 1401, 2206, 3102
Hpy188III TCNNGA 8 cut(s) 152, 261, 750, 1436, 1511, 2382, 2640, 2801
Hpy8I GTNNAC 6 cut(s) 360, 1002, 1360, 1401, 2206, 3102
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 5 cut(s) 32, 215, 2155, 2594, 2951
HpyCH4III ACNGT 5 cut(s) 778, 1364, 1895, 2743, 3099
HpyCH4IV ACGT 1 cut(s) 3160
HpyF10VI GCNNNNNNNGC 9 cut(s) 82, 247, 473, 768, 866, 1088, 1880, 1973, 2097
HpyF3I CTNAG 7 cut(s) 892, 1048, 1404, 1743, 2802, 2990, 3020
HpySE526I ACGT 1 cut(s) 3160
HspAI GCGC 4 cut(s) 476, 784, 1974, 2052
KflI GGGWCCC 1 cut(s) 1444
KspAI GTTAAC 2 cut(s) 1360, 1401
LmnI GCTCC 1 cut(s) 906
Lsp1109I GCAGC 5 cut(s) 798, 1248, 1858, 2037, 2260
MabI ACCWGGT 1 cut(s) 949
MaeII ACGT 1 cut(s) 3160
MaeIII GTNAC 8 cut(s) 296, 1043, 1383, 1589, 1784, 1889, 2675, 2789
MfeI CAATTG 1 cut(s) 1668
MflI RGATCY 4 cut(s) 283, 2183, 2662, 2980
MlyI GAGTC 1 cut(s) 1078
MmeI TCCRAC 2 cut(s) 1820, 2467
Mph1103I ATGCAT 4 cut(s) 185, 499, 587, 3029
MroXI GAANNNNTTC 3 cut(s) 206, 2585, 2824
MslI CAYNNNNRTG 2 cut(s) 1077, 1390
MspCI CTTAAG 1 cut(s) 2176
MspI CCGG 1 cut(s) 1936
MspR9I CCNGG 4 cut(s) 951, 1323, 1341, 1422
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 2 cut(s) 1069, 2478
MvaI CCWGG 4 cut(s) 951, 1323, 1341, 1422
MwoI GCNNNNNNNGC 9 cut(s) 82, 247, 473, 768, 866, 1088, 1880, 1973, 2097
NdeI CATATG 1 cut(s) 2785
NlaIV GGNNCC 4 cut(s) 1445, 1446, 1733, 2982
NmuCI GTSAC 1 cut(s) 1383
NsiI ATGCAT 4 cut(s) 185, 499, 587, 3029
NspI RCATGY 4 cut(s) 187, 2566, 2797, 3035
OliI CACNNNNGTG 1 cut(s) 1390
PaeI GCATGC 2 cut(s) 187, 2566
PciI ACATGT 2 cut(s) 2793, 3031
PctI GAATGC 2 cut(s) 1069, 2478
PdmI GAANNNNTTC 3 cut(s) 206, 2585, 2824
PkrI GCNGC 6 cut(s) 788, 1263, 1476, 1873, 2027, 2275
PleI GAGTC 1 cut(s) 1077
PpsI GAGTC 1 cut(s) 1077
PpuMI RGGWCCY 3 cut(s) 1424, 1444, 2164
PscI ACATGT 2 cut(s) 2793, 3031
PsiI TTATAA 3 cut(s) 60, 1055, 2334
Psp5II RGGWCCY 3 cut(s) 1424, 1444, 2164
Psp6I CCWGG 4 cut(s) 949, 1321, 1339, 1420
PspGI CCWGG 4 cut(s) 949, 1321, 1339, 1420
PspN4I GGNNCC 4 cut(s) 1445, 1446, 1733, 2982
PspPI GGNCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
PspPPI RGGWCCY 3 cut(s) 1424, 1444, 2164
PstI CTGCAG 1 cut(s) 22
PsuI RGATCY 4 cut(s) 283, 2183, 2662, 2980
RsaI GTAC 7 cut(s) 71, 323, 346, 775, 780, 837, 1115
RsaNI GTAC 7 cut(s) 70, 322, 345, 774, 779, 836, 1114
RseI CAYNNNNRTG 2 cut(s) 1077, 1390
SatI GCNGC 6 cut(s) 787, 1262, 1475, 1872, 2026, 2274
Sau96I GGNCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 1 cut(s) 1078
ScrFI CCNGG 4 cut(s) 951, 1323, 1341, 1422
SexAI ACCWGGT 1 cut(s) 949
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
SmiMI CAYNNNNRTG 2 cut(s) 1077, 1390
SmlI CTYRAG 6 cut(s) 231, 259, 1039, 2176, 2610, 2638
SmoI CTYRAG 6 cut(s) 231, 259, 1039, 2176, 2610, 2638
SphI GCATGC 2 cut(s) 187, 2566
SsiI CCGC 3 cut(s) 873, 1474, 2084
SspI AATATT 2 cut(s) 756, 1531
StyD4I CCNGG 4 cut(s) 949, 1321, 1339, 1420
StyI CCWWGG 1 cut(s) 2984
TaaI ACNGT 5 cut(s) 778, 1364, 1895, 2743, 3099
TaiI ACGT 1 cut(s) 3163
TaqI TCGA 5 cut(s) 219, 609, 879, 1510, 2228
TatI WGTACW 3 cut(s) 773, 778, 835
TauI GCSGC 1 cut(s) 1477
TscAI CASTG 7 cut(s) 469, 719, 948, 1222, 1422, 1600, 3102
TseFI GTSAC 1 cut(s) 1383
TseI GCWGC 5 cut(s) 786, 1261, 1871, 2025, 2273
Tsp45I GTSAC 1 cut(s) 1383
TspGWI ACGGA 1 cut(s) 1814
TspRI CASTG 7 cut(s) 469, 719, 948, 1222, 1422, 1600, 3102
Vha464I CTTAAG 1 cut(s) 2176
VpaK11BI GGWCC 6 cut(s) 50, 1424, 1444, 1732, 2164, 2324
XbaI TCTAGA 1 cut(s) 749
XceI RCATGY 4 cut(s) 187, 2566, 2797, 3035
XmnI GAANNNNTTC 3 cut(s) 206, 2585, 2824
ZrmI AGTACT 1 cut(s) 837
Zsp2I ATGCAT 4 cut(s) 185, 499, 587, 3029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.