Rw5G016240

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000096
Physical Location & Seq
Forward (+)
20355460 .. 20358520
3061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016240.1

Sequence Viewer

Length: 2271 bp
ATGATCAACTTATTGTGCTTGCTCATCAGTTTCTCAAGCTTCTGGACTTGTCATGGTGCAGCTGCACTGTTCATTGGAGACACGCTTAATCCAGGGGAGGCCCTCAATTCCTCGAGCTTTATGGTCTCCGCTTCGGGGGAGTTTACGTTGGGTTTCAACTCTGGCTACCTCATTCTGAGGTGCAAGAACTATACGGTTTGGGTTGCCAACCTGCACACTCCGATTTCAGACCCTTCGTCGGCGCTTCTCACCTTGGACAAGAACAGAACTGTCCTGCAAATTACCCTGAAAGGCAAGAATCCCATCGTTCTTTACTCCCCTTCTCATGATAATAGTACCAATAATGTTGTGGCTACCCTGTTAGATTCTGGCAATTTAGTCCTACGAGAGGTCAATCAGAACGTTTCAAAGTCCCCGTTGTGGCAAAGCTTTGATGGTCCTAATGTGGCTCTTAGGCCGGGGATGAAATTAGGTTTTAATGCTGGTCTAAGTATAACTAGCAACAGTATGTCGATTTGGAGTTGGCAGGAAAATCAGAACAGCCCGGGGCCAGGGTCTTTCAGCCTTTATTGGGATTCCGGCACACGCCAATTGCAAGTTAGGAGAAACGGGGATCTTTGGTGGACTAGCGAAACATTGAGAAGTGGAAGATACGAGTATGCTAATTTCCGTAATAAATCCAAGGTCTACAATTTTAGCATGGTTTCATATGAGAATGGAGACTACTTCAGTTACACTGTCGTTGGTGATCCAAATGGCAGATCAGCCTGGGTGCTAGACACAGGTGGGGAGCTTTATGATGTTAACTATAACATTTCTATTGGGTCAGCAGGTGACTGTGATGGCTATAATTATCACACCACCTATAGCTCTGTCGATGGCTATAACTCCGTTGGTGGGACGTGCCAGAGATGGAGTGGCCGGGCCACTCTTTATTGTCCTGTGGCAACATCCTCTGGTGCATTTATTCAGCCGACTGAAAGGCGCTCCTTTAGACCGATCAGTATGAACATGTCTATCAATTCAACATGGGAGGAGACAAATTCAAATGCCTCACATAAGTGGATATGGATTGGTATTGCAATTGCGGCTGTTCTTCTGGCGCTGGTGCTTTGCAGCATCTGCTACCTAATACGAAAGAGAAAACTTGCAGGTTTTAGTGAGAACCAAATAAACATCCAGGATATGCTGAACATCATGAAATCTAATAGACCTACTGATGCTAATGGGCTTCAAAATGATGGAAAGAGGGGACAAGATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACGTGCAACTTCTCTAATGAAAATAAGCTCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGGAAATTGGTGACTGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAACGTTCAGGGCAGGGATTATATGAGTTTAAGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGCTGTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAAAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAGCAGTTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAATATTCCAGAATGAAAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACGAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACACGTGGATATATGTCCCCTGAGTATATTATGGGGGGAAAATTTTCCATAAAATCTGATGTCTATAGTTTCGGTGTATTGATGCTTGAAATCCTAAGTGGTAGAAGAAACAACAGCTTCTACAATGATGATGGCGCCCTCAATTTAGTTGGTTATGCATGGGCTTTATGGAAAGAAGGTGCAGGACTAGAACTAATGGATCCAACACTAGGCGATTCATGTGATAAAGATCATTTGTTGAGGTGCATCCATGTTGGTTTGCTTTGTGTAGAGGAAAATGCAGCCAGTCGACCAAAGATGTTAGATGTGATATCTATGCTGACTAATGAAAGCATGTCACTGCCAGTACCTACAAAACCAGCATTTTGTACAGAAAGAAATGTCATCACAACTACTGTAGGTGGAAACGGACCGGAAGTGGTGGTATCAGTAAATGGTATCTCCAATTCTGATTATGATGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

756

Amino Acids

84.08

Weight (kDa)

6.3

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 65 - 175 4e-19 D-mannose binding lectin
Pkinase PF00069 437 - 637 1.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 637 8.8e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1353
AarI CACCTGC 1 cut(s) 821
Acc36I ACCTGC 3 cut(s) 219, 821, 1142
AccB1I GGYRCC 1 cut(s) 1937
AccI GTMKAC 2 cut(s) 687, 2092
AciI CCGC 2 cut(s) 129, 1088
AclI AACGTT 2 cut(s) 402, 1405
AclWI GGATC 4 cut(s) 621, 743, 1997, 2010
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 3 cut(s) 1042, 1731, 1844
AcuI CTGAAG 1 cut(s) 712
AcvI CACGTG 2 cut(s) 1299, 1808
AcyI GRCGYC 1 cut(s) 1938
AfaI GTAC 4 cut(s) 337, 1532, 2151, 2173
AfiI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 551, 571, 897, 2012
AflIII ACRYGT 2 cut(s) 1011, 1805
AhdI GACNNNNNGTC 1 cut(s) 235
AjiI CACGTC 1 cut(s) 903
AjnI CCWGG 4 cut(s) 91, 550, 767, 1179
AjuI GAANNNNNNNTTGG 2 cut(s) 1323, 1355
AleI CACNNNNGTG 1 cut(s) 1060
Alw26I GTCTC 4 cut(s) 72, 130, 714, 1031
AlwI GGATC 4 cut(s) 621, 743, 1997, 2010
AlwNI CAGNNNCTG 1 cut(s) 1122
Ama87I CYCGRG 2 cut(s) 112, 544
AoxI GGCC 5 cut(s) 99, 455, 548, 919, 924
ApeKI GCWGC 6 cut(s) 59, 62, 1116, 1292, 1585, 2084
ApoI RAATTY 3 cut(s) 1042, 1731, 1844
Asp700I GAANNNNTTC 1 cut(s) 1846
AspLEI GCGC 4 cut(s) 244, 987, 1105, 1940
AspS9I GGNCC 6 cut(s) 100, 437, 548, 924, 1343, 2213
AsuC2I CCSGG 4 cut(s) 459, 545, 546, 923
AsuHPI GGTGA 5 cut(s) 241, 758, 845, 1378, 1517
AvaI CYCGRG 2 cut(s) 112, 544
AvaII GGWCC 3 cut(s) 437, 1343, 2213
BamHI GGATCC 1 cut(s) 2002
BanI GGYRCC 1 cut(s) 1937
BbrPI CACGTG 2 cut(s) 1299, 1808
BbvI GCAGC 6 cut(s) 49, 71, 1128, 1279, 1597, 2096
BccI CCATC 8 cut(s) 311, 428, 836, 872, 906, 1235, 1928, 2255
BciT130I CCWGG 4 cut(s) 93, 552, 769, 1181
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 459, 545, 546, 923
BcoDI GTCTC 4 cut(s) 72, 130, 714, 1031
BfaI CTAG 6 cut(s) 498, 627, 776, 1575, 1991, 2012
BfmI CTRYAG 3 cut(s) 865, 1867, 2199
BfoI RGCGCY 4 cut(s) 245, 988, 1106, 1941
BfuAI ACCTGC 3 cut(s) 219, 821, 1142
BisI GCNGC 7 cut(s) 60, 63, 1089, 1117, 1293, 1586, 2085
BlsI GCNGC 7 cut(s) 61, 64, 1090, 1118, 1294, 1587, 2086
Bme1390I CCNGG 8 cut(s) 93, 459, 545, 546, 552, 769, 923, 1181
Bme18I GGWCC 3 cut(s) 437, 1343, 2213
BmeRI GACNNNNNGTC 1 cut(s) 235
BmeT110I CYCGRG 2 cut(s) 112, 544
BmgBI CACGTC 1 cut(s) 903
BmgT120I GGNCC 6 cut(s) 100, 437, 548, 924, 1343, 2213
BmiI GGNNCC 3 cut(s) 549, 1939, 2004
BmrFI CCNGG 8 cut(s) 93, 459, 545, 546, 552, 769, 923, 1181
BmsI GCATC 6 cut(s) 1128, 1210, 1289, 1568, 1875, 2058
BpmI CTGGAG 1 cut(s) 1445
BpuEI CTTGAG 2 cut(s) 19, 1617
BpuMI CCSGG 4 cut(s) 459, 545, 546, 923
BsaAI YACGTR 2 cut(s) 1299, 1808
BsaBI GATNNNNATC 1 cut(s) 2031
BsaHI GRCGYC 1 cut(s) 1938
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 8 cut(s) 92, 252, 458, 544, 545, 551, 681, 768
BsaWI WCCGGW 1 cut(s) 2215
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 551, 571, 897, 2012
Bse1I ACTGG 4 cut(s) 1375, 1601, 2088, 2147
Bse8I GATNNNNATC 1 cut(s) 2031
BseBI CCWGG 4 cut(s) 93, 552, 769, 1181
BseDI CCNNGG 8 cut(s) 92, 252, 458, 544, 545, 551, 681, 768
BseGI GGATG 5 cut(s) 468, 950, 1176, 1521, 2049
BseJI GATNNNNATC 1 cut(s) 2031
BseLI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 551, 571, 897, 2012
BseMII CTCAG 2 cut(s) 167, 1815
BseNI ACTGG 4 cut(s) 1375, 1601, 2088, 2147
BseRI GAGGAG 1 cut(s) 1049
BseXI GCAGC 6 cut(s) 49, 71, 1128, 1279, 1597, 2096
BsgI GTGCAG 4 cut(s) 48, 78, 197, 2004
BshFI GGCC 5 cut(s) 101, 457, 550, 921, 926
BshNI GGYRCC 1 cut(s) 1937
BsiHKCI CYCGRG 2 cut(s) 112, 544
BsiSI CCGG 5 cut(s) 458, 545, 579, 922, 2216
BslFI GGGAC 5 cut(s) 397, 913, 1266, 1804, 1816
BslI CCNNNNNNNGG 8 cut(s) 135, 238, 388, 420, 551, 571, 897, 2012
BsmAI GTCTC 4 cut(s) 72, 130, 714, 1031
BsmFI GGGAC 5 cut(s) 397, 913, 1266, 1804, 1816
BsmI GAATGC 1 cut(s) 1497
BsnI GGCC 5 cut(s) 101, 457, 550, 921, 926
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 112, 544
Bsp1407I TGTACA 1 cut(s) 2171
Bsp143I GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
BspACI CCGC 2 cut(s) 129, 1088
BspANI GGCC 5 cut(s) 101, 457, 550, 921, 926
BspCNI CTCAG 2 cut(s) 168, 1816
BspHI TCATGA 2 cut(s) 325, 1197
BspLI GGNNCC 3 cut(s) 549, 1939, 2004
BspMI ACCTGC 3 cut(s) 219, 821, 1142
BspPI GGATC 4 cut(s) 621, 743, 1997, 2010
BspT107I GGYRCC 1 cut(s) 1937
BspTNI GGTCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 2171
BsrI ACTGG 4 cut(s) 1375, 1601, 2088, 2147
BssECI CCNNGG 8 cut(s) 92, 252, 458, 544, 545, 551, 681, 768
BssMI GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
BssNI GRCGYC 1 cut(s) 1938
BssT1I CCWWGG 2 cut(s) 252, 681
Bst2UI CCWGG 4 cut(s) 93, 552, 769, 1181
Bst4CI ACNGT 8 cut(s) 69, 196, 271, 506, 739, 839, 1607, 2200
Bst6I CTCTTC 1 cut(s) 1503
BstACI GRCGYC 1 cut(s) 1938
BstAPI GCANNNNNTGC 1 cut(s) 1122
BstAUI TGTACA 1 cut(s) 2171
BstBAI YACGTR 2 cut(s) 1299, 1808
BstC8I GCNNGC 2 cut(s) 20, 1583
BstDEI CTNAG 5 cut(s) 176, 452, 488, 1824, 1898
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 5 cut(s) 468, 950, 1176, 1521, 2049
BstH2I RGCGCY 4 cut(s) 245, 988, 1106, 1941
BstHHI GCGC 4 cut(s) 244, 987, 1105, 1940
BstKTI GATC 7 cut(s) 6, 616, 751, 764, 1002, 2005, 2035
BstMAI GTCTC 4 cut(s) 72, 130, 714, 1031
BstMBI GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
BstMWI GCNNNNNNNGC 2 cut(s) 1088, 1122
BstNI CCWGG 4 cut(s) 93, 552, 769, 1181
BstNSI RCATGY 3 cut(s) 1015, 1537, 2140
BstSCI CCNGG 8 cut(s) 91, 457, 543, 544, 550, 767, 921, 1179
BstSFI CTRYAG 3 cut(s) 865, 1867, 2199
BstV1I GCAGC 6 cut(s) 49, 71, 1128, 1279, 1597, 2096
BstX2I RGATCY 2 cut(s) 613, 2002
BstYI RGATCY 2 cut(s) 613, 2002
BsuRI GGCC 5 cut(s) 101, 457, 550, 921, 926
BtrI CACGTC 1 cut(s) 903
BtsCI GGATG 5 cut(s) 468, 950, 1176, 1521, 2049
BtsI GCAGTG 2 cut(s) 1392, 2141
BtsIMutI CAGTG 4 cut(s) 65, 735, 1392, 2141
BveI ACCTGC 3 cut(s) 219, 821, 1142
Cac8I GCNNGC 2 cut(s) 20, 1583
CaiI CAGNNNCTG 1 cut(s) 1122
CciI TCATGA 2 cut(s) 325, 1197
CfoI GCGC 4 cut(s) 244, 987, 1105, 1940
Cfr13I GGNCC 6 cut(s) 100, 437, 548, 924, 1343, 2213
Cfr9I CCCGGG 1 cut(s) 544
CpoI CGGWCCG 1 cut(s) 2213
Csp6I GTAC 4 cut(s) 336, 1531, 2150, 2172
CspI CGGWCCG 1 cut(s) 2213
CviQI GTAC 4 cut(s) 336, 1531, 2150, 2172
DdeI CTNAG 5 cut(s) 176, 452, 488, 1824, 1898
DinI GGCGCC 1 cut(s) 1939
DpnI GATC 7 cut(s) 5, 615, 750, 763, 1001, 2004, 2034
DpnII GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
DriI GACNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 1503
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 1503
Eco130I CCWWGG 2 cut(s) 252, 681
Eco31I GGTCTC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 2115
Eco47I GGWCC 3 cut(s) 437, 1343, 2213
Eco57I CTGAAG 1 cut(s) 712
Eco72I CACGTG 2 cut(s) 1299, 1808
Eco88I CYCGRG 2 cut(s) 112, 544
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 4 cut(s) 91, 550, 767, 1179
EcoRV GATATC 1 cut(s) 2115
EcoT14I CCWWGG 2 cut(s) 252, 681
EcoT22I ATGCAT 2 cut(s) 1282, 1963
EgeI GGCGCC 1 cut(s) 1939
EheI GGCGCC 1 cut(s) 1939
ErhI CCWWGG 2 cut(s) 252, 681
FalI AAGNNNNNCTT 2 cut(s) 1626, 1658
FaqI GGGAC 5 cut(s) 397, 913, 1266, 1804, 1816
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 687, 2092
Fnu4HI GCNGC 7 cut(s) 60, 63, 1089, 1117, 1293, 1586, 2085
FokI GGATG 5 cut(s) 475, 937, 1163, 1528, 2036
Fsp4HI GCNGC 7 cut(s) 60, 63, 1089, 1117, 1293, 1586, 2085
FspBI CTAG 6 cut(s) 498, 627, 776, 1575, 1991, 2012
GlaI GCGC 4 cut(s) 243, 986, 1104, 1939
GluI GCNGC 7 cut(s) 60, 63, 1089, 1117, 1293, 1586, 2085
GsuI CTGGAG 1 cut(s) 1445
HaeII RGCGCY 4 cut(s) 245, 988, 1106, 1941
HaeIII GGCC 5 cut(s) 101, 457, 550, 921, 926
HapII CCGG 5 cut(s) 458, 545, 579, 922, 2216
HhaI GCGC 4 cut(s) 244, 987, 1105, 1940
Hin1I GRCGYC 1 cut(s) 1938
Hin6I GCGC 4 cut(s) 242, 985, 1103, 1938
HinP1I GCGC 4 cut(s) 242, 985, 1103, 1938
HincII GTYRAC 2 cut(s) 805, 2093
HindII GTYRAC 2 cut(s) 805, 2093
HindIII AAGCTT 3 cut(s) 37, 427, 1546
HinfI GANTC 5 cut(s) 298, 365, 575, 1568, 2018
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 5 cut(s) 458, 545, 579, 922, 2216
HphI GGTGA 5 cut(s) 241, 758, 845, 1378, 1517
Hpy166II GTNNAC 6 cut(s) 144, 624, 688, 805, 1640, 2093
Hpy188I TCNGA 9 cut(s) 177, 222, 229, 399, 537, 1328, 1738, 1861, 2254
Hpy188III TCNNGA 5 cut(s) 43, 326, 1198, 1662, 1762
Hpy8I GTNNAC 6 cut(s) 144, 624, 688, 805, 1640, 2093
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 243, 330, 1325, 1616, 1973
HpyCH4III ACNGT 8 cut(s) 69, 196, 271, 506, 739, 839, 1607, 2200
HpyCH4IV ACGT 7 cut(s) 146, 402, 902, 1298, 1405, 1807, 2266
HpyF10VI GCNNNNNNNGC 2 cut(s) 1088, 1122
HpyF3I CTNAG 5 cut(s) 176, 452, 488, 1824, 1898
HpySE526I ACGT 7 cut(s) 146, 402, 902, 1298, 1405, 1807, 2266
Hsp92I GRCGYC 1 cut(s) 1938
HspAI GCGC 4 cut(s) 242, 985, 1103, 1938
KasI GGCGCC 1 cut(s) 1937
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
LmnI GCTCC 2 cut(s) 790, 992
Lsp1109I GCAGC 6 cut(s) 49, 71, 1128, 1279, 1597, 2096
LweI GCATC 6 cut(s) 1128, 1210, 1289, 1568, 1875, 2058
MaeI CTAG 6 cut(s) 498, 627, 776, 1575, 1991, 2012
MaeII ACGT 7 cut(s) 146, 402, 902, 1298, 1405, 1807, 2266
MaeIII GTNAC 4 cut(s) 731, 833, 1366, 2139
MalI GATC 7 cut(s) 5, 615, 750, 763, 1001, 2004, 2034
MboI GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
MboII GAAGA 5 cut(s) 660, 1088, 1520, 1804, 1920
MfeI CAATTG 2 cut(s) 590, 1083
MflI RGATCY 2 cut(s) 613, 2002
Mly113I GGCGCC 1 cut(s) 1938
MmeI TCCRAC 1 cut(s) 2030
Mph1103I ATGCAT 2 cut(s) 1282, 1963
MroXI GAANNNNTTC 1 cut(s) 1846
MseI TTAA 6 cut(s) 87, 477, 804, 1262, 1431, 2269
MslI CAYNNNNRTG 1 cut(s) 1060
MspA1I CMGCKG 2 cut(s) 62, 1483
MspI CCGG 5 cut(s) 458, 545, 579, 922, 2216
MspR9I CCNGG 8 cut(s) 93, 459, 545, 546, 552, 769, 923, 1181
MunI CAATTG 2 cut(s) 590, 1083
Mva1269I GAATGC 1 cut(s) 1497
MvaI CCWGG 4 cut(s) 93, 552, 769, 1181
MwoI GCNNNNNNNGC 2 cut(s) 1088, 1122
NarI GGCGCC 1 cut(s) 1938
NciI CCSGG 4 cut(s) 459, 545, 546, 923
NdeI CATATG 1 cut(s) 709
NdeII GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
NlaIV GGNNCC 3 cut(s) 549, 1939, 2004
NmuCI GTSAC 3 cut(s) 833, 1366, 2139
NsiI ATGCAT 2 cut(s) 1282, 1963
NspI RCATGY 3 cut(s) 1015, 1537, 2140
OliI CACNNNNGTG 1 cut(s) 1060
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 2 cut(s) 325, 1197
PaqCI CACCTGC 1 cut(s) 821
PciI ACATGT 1 cut(s) 1011
PctI GAATGC 1 cut(s) 1497
PdmI GAANNNNTTC 1 cut(s) 1846
PfeI GAWTC 5 cut(s) 298, 365, 575, 1568, 2018
PfoI TCCNGGA 1 cut(s) 1179
PkrI GCNGC 7 cut(s) 61, 64, 1090, 1118, 1294, 1587, 2086
PluTI GGCGCC 1 cut(s) 1941
PmaCI CACGTG 2 cut(s) 1299, 1808
PmlI CACGTG 2 cut(s) 1299, 1808
Ppu21I YACGTR 2 cut(s) 1299, 1808
PscI ACATGT 1 cut(s) 1011
PsiI TTATAA 1 cut(s) 1353
Psp1406I AACGTT 2 cut(s) 402, 1405
Psp6I CCWGG 4 cut(s) 91, 550, 767, 1179
PspCI CACGTG 2 cut(s) 1299, 1808
PspGI CCWGG 4 cut(s) 91, 550, 767, 1179
PspN4I GGNNCC 3 cut(s) 549, 1939, 2004
PspPI GGNCC 6 cut(s) 100, 437, 548, 924, 1343, 2213
PspXI VCTCGAGB 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 1122
PsuI RGATCY 2 cut(s) 613, 2002
PvuII CAGCTG 2 cut(s) 62, 1483
RsaI GTAC 4 cut(s) 337, 1532, 2151, 2173
RsaNI GTAC 4 cut(s) 336, 1531, 2150, 2172
RseI CAYNNNNRTG 1 cut(s) 1060
Rsr2I CGGWCCG 1 cut(s) 2213
RsrII CGGWCCG 1 cut(s) 2213
SalI GTCGAC 1 cut(s) 2091
SaqAI TTAA 6 cut(s) 87, 477, 804, 1262, 1431, 2269
SatI GCNGC 7 cut(s) 60, 63, 1089, 1117, 1293, 1586, 2085
Sau3AI GATC 7 cut(s) 3, 613, 748, 761, 999, 2002, 2032
Sau96I GGNCC 6 cut(s) 100, 437, 548, 924, 1343, 2213
ScrFI CCNGG 8 cut(s) 93, 459, 545, 546, 552, 769, 923, 1181
SfaNI GCATC 6 cut(s) 1128, 1210, 1289, 1568, 1875, 2058
SfcI CTRYAG 3 cut(s) 865, 1867, 2199
SfoI GGCGCC 1 cut(s) 1939
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 3 cut(s) 437, 1343, 2213
SlaI CTCGAG 1 cut(s) 112
SmaI CCCGGG 1 cut(s) 546
SmiMI CAYNNNNRTG 1 cut(s) 1060
SmlI CTYRAG 3 cut(s) 34, 112, 1632
SmoI CTYRAG 3 cut(s) 34, 112, 1632
SsiI CCGC 2 cut(s) 129, 1088
SspDI GGCGCC 1 cut(s) 1937
SspI AATATT 1 cut(s) 1658
SspMI CTAG 6 cut(s) 498, 627, 776, 1575, 1991, 2012
StyD4I CCNGG 8 cut(s) 91, 457, 543, 544, 550, 767, 921, 1179
StyI CCWWGG 2 cut(s) 252, 681
TaaI ACNGT 8 cut(s) 69, 196, 271, 506, 739, 839, 1607, 2200
TaiI ACGT 7 cut(s) 149, 405, 905, 1301, 1408, 1810, 2269
TaqI TCGA 4 cut(s) 113, 512, 876, 2092
TaqII GACCGA 1 cut(s) 1012
TatI WGTACW 2 cut(s) 1530, 2171
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 5 cut(s) 298, 365, 575, 1568, 2018
Tru1I TTAA 6 cut(s) 87, 477, 804, 1262, 1431, 2269
Tru9I TTAA 6 cut(s) 87, 477, 804, 1262, 1431, 2269
TscAI CASTG 4 cut(s) 72, 742, 1392, 2148
TseFI GTSAC 3 cut(s) 833, 1366, 2139
TseI GCWGC 6 cut(s) 59, 62, 1116, 1292, 1585, 2084
Tsp45I GTSAC 3 cut(s) 833, 1366, 2139
TspGWI ACGGA 3 cut(s) 659, 880, 2226
TspMI CCCGGG 1 cut(s) 544
TspRI CASTG 4 cut(s) 72, 742, 1392, 2148
VpaK11BI GGWCC 3 cut(s) 437, 1343, 2213
XagI CCTNNNNNAGG 1 cut(s) 386
XapI RAATTY 3 cut(s) 1042, 1731, 1844
XceI RCATGY 3 cut(s) 1015, 1537, 2140
XcmI CCANNNNNNNNNTGG 2 cut(s) 346, 914
XhoI CTCGAG 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 544
XmiI GTMKAC 2 cut(s) 687, 2092
XmnI GAANNNNTTC 1 cut(s) 1846
XspI CTAG 6 cut(s) 498, 627, 776, 1575, 1991, 2012
Zsp2I ATGCAT 2 cut(s) 1282, 1963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.