Rh5CG195600

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000096
Physical Location & Seq
Forward (+)
19461446 .. 19464788
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG195600.1

Sequence Viewer

Length: 2493 bp
ATGGTTAACAGCATTCCAATGATCAACTTATTGTGCTTGCTCATCAGTTTCTCAAGCTTCTGGACTTGTCATGGTGCAGCTGCACTGTTCATTGGAGACACGCTTAATCCAGGGGAGGCCCTCAATTCCTCGAGCTTTATGGTCTCCGCTTCGGGGGAGTTTACGTTGGGTTTCAACTCCGGCTACCTCATTCTGAGGTGCAAGAACTATGCGGTTTGGGTTGCCAACCTGCACACTTCGATTTCAGACCCTTCGTTGGCGCTTCTCACCTTGGACAAGAACAGAACTGTCCTGCAAATTACCCTGAAAGGCAAGAATCCCATCGTTCTTTACTCCCCTCATTATGATAATAGTACCAATAATGTTGTGGCTACCCTGTTAGATTCTGGCAATTTAGTCCTACGAGAGGTCAATCAGAACGTTTCAAACTCTCCGTTGTGGCAAAGCTTTGATGGTCCTAATGTGGCTCTTAGGCCGGGGATGAAATTAGGTTTTGATGCTGGTCTAAGTATAACTAGCAACAGTATGTCGATTTGGAGTTGGCAGGAAAATCAGAACAGCCCGGGGCCAGGGTCTTTCAGCCTTTATTGGGATTCCGGCACACGCCAATTGCAAGTTAGGAGAAACGGGGATCTTTGGTGGACTAGCGAAACATTGAAAAGTGGAATATACGAGTATGCTAATTTCCGTAATAAATCCAAGGTCTACAATTTTAGCGTGGTTTCATATGAGAATGGAGACTACTTCAGTTACACTGTCGTTGGTGATCCAAATGGCAGATCAGCCTGGGTGCTAGACACAGGTGGGGAGCTTTATGATGTTAACTATAATATTTCTATTGGGTCAGCAGGTGACTGTGATGGCTATAATTATCACACCACCTATAGCTATGTCGACGGCTATAACCCCGTTGGTGGGAAGTGCCAGAGATGGAGTGGCCGGGCCACTCTTTATTGTCCTGTGGCAACATCCTCTGGTGCATTTATTCAGCCGACAGAAAGGCGCTCCTTTAGACCAATCAGTATGAACAAGTCTATCAATTCAACATGGGAGGAGACAAATTCAAGTCGATATGGGATTTATGAATGTATGGCTACTTGTTGGATGGACTGTGACTGCCTTGGTTTCGAATTTCTCTTTGATAATCAGACTGGATGTCGATTTTGGAGCGTAAAGTATTCTCAGGTCCTTGAAGATCCGGATAGTTCTGCAACTAAATATATTATACCAGGACTAGTCGCTACGTATAAACCGCAATCTCGAAGAAACATAATTGATGCATCACATAAGTGGATATGGATTGGTATTGCAATTGCGGCTGTTCTTCTGGCGCTGGTGCTTTGTAGCATCTGCTACCTAATACGAAAAAGAAAACTTGCAGGTGAGAACCAAATAAACATCCAGGATATGCTGAACGTCATGAAATCTAATAGACCTACTGATGCTAATGGGCTTCAAAATGATGGAAAGAGGGGACAAGATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACTTGCAACTTCTCTAATGAAAATAAGCTCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGGAAATTGGTGACTGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAACGTTCAGGGCAGGGATTATATGAGTTTAAGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGCTGTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAAAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAGCAGTTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAGTATTCCAGAATGAAAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACGAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACACGTGGATATATGTCCCCTGAGTATATTATGGGGGGAAAATTTTCCATAAAATCTGATGTCTATAGTTTCGGTGTATTGATGCTTGAAATCCTAAGTGGTAGAAGAAACAACAGCTTCTACAATGATGATCGCGCCCTCAATTTAGTTGGTTATGCATGGGCGTTATGGAAAGAAGGTGCAGGACTAGAACTAATGGATCCAACACTTGGCGATTCATGTGATAAACATCATTTGTTGAGGTGCATCCATGTTGGTTTGCTTTGTGTAGAGGAAAATGCAGCCAGTCGACCAAAGATGTTAGATGTGATATCTATGCTGACTAATGAAAGCATGTCACTGCCAGTACCTACAAAACCAGCATTTTGTACAGAAAGAAATGTCATCACAACTACTGTAGGTGGAAACGGACCGGAAGTGGTGGTATCGGTAAATGGTATCTCCAATTCTGATTTTGATGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

830

Amino Acids

92.75

Weight (kDa)

6.23

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 181 1.2e-16 D-mannose binding lectin
Pkinase PF00069 511 - 711 2.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 513 - 712 8e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1575
AarI CACCTGC 2 cut(s) 839, 1370
Acc36I ACCTGC 3 cut(s) 237, 839, 1370
AccB7I CCANNNNNTGG 1 cut(s) 2234
AccI GTMKAC 3 cut(s) 705, 894, 2314
AccII CGCG 1 cut(s) 2160
AccIII TCCGGA 1 cut(s) 1198
AciI CCGC 4 cut(s) 147, 212, 1253, 1316
AclI AACGTT 2 cut(s) 420, 1627
AclWI GGATC 5 cut(s) 639, 761, 1190, 2219, 2232
AcoI YGGCCR 1 cut(s) 937
AcsI RAATTY 4 cut(s) 1060, 1130, 1953, 2066
AcuI CTGAAG 1 cut(s) 730
AcvI CACGTG 1 cut(s) 2030
AfaI GTAC 4 cut(s) 355, 1754, 2373, 2395
AfiI CCNNNNNNNGG 8 cut(s) 153, 256, 406, 569, 589, 914, 915, 2234
AflIII ACRYGT 1 cut(s) 2027
AhdI GACNNNNNGTC 1 cut(s) 1155
AhlI ACTAGT 1 cut(s) 1234
AjnI CCWGG 5 cut(s) 109, 568, 785, 1228, 1401
AjuI GAANNNNNNNTTGG 2 cut(s) 1545, 1577
AleI CACNNNNGTG 1 cut(s) 1288
Alw26I GTCTC 4 cut(s) 90, 148, 732, 1049
AlwI GGATC 5 cut(s) 639, 761, 1190, 2219, 2232
Ama87I CYCGRG 2 cut(s) 130, 562
Aor13HI TCCGGA 1 cut(s) 1198
AoxI GGCC 5 cut(s) 117, 473, 566, 937, 942
ApeKI GCWGC 5 cut(s) 77, 80, 1514, 1807, 2306
ApoI RAATTY 4 cut(s) 1060, 1130, 1953, 2066
Asp700I GAANNNNTTC 1 cut(s) 2068
AspLEI GCGC 4 cut(s) 262, 1005, 1333, 2162
AspS9I GGNCC 7 cut(s) 118, 455, 566, 942, 1186, 1565, 2435
AsuC2I CCSGG 4 cut(s) 477, 563, 564, 941
AsuHPI GGTGA 6 cut(s) 259, 776, 863, 1394, 1600, 1739
AsuII TTCGAA 1 cut(s) 1128
AvaI CYCGRG 2 cut(s) 130, 562
AvaII GGWCC 4 cut(s) 455, 1186, 1565, 2435
BamHI GGATCC 1 cut(s) 2224
BbrPI CACGTG 1 cut(s) 2030
BbvI GCAGC 5 cut(s) 67, 89, 1501, 1819, 2318
BccI CCATC 7 cut(s) 329, 446, 854, 924, 1099, 1457, 2477
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 5 cut(s) 111, 570, 787, 1230, 1403
BclI TGATCA 1 cut(s) 21
BcnI CCSGG 4 cut(s) 477, 563, 564, 941
BcoDI GTCTC 4 cut(s) 90, 148, 732, 1049
BcuI ACTAGT 1 cut(s) 1234
BfaI CTAG 6 cut(s) 516, 645, 794, 1235, 1797, 2213
BfmI CTRYAG 3 cut(s) 883, 2089, 2421
BfoI RGCGCY 3 cut(s) 263, 1006, 1334
BfuAI ACCTGC 3 cut(s) 237, 839, 1370
BisI GCNGC 6 cut(s) 78, 81, 1317, 1515, 1808, 2307
BlsI GCNGC 6 cut(s) 79, 82, 1318, 1516, 1809, 2308
Bme1390I CCNGG 9 cut(s) 111, 477, 563, 564, 570, 787, 941, 1230, 1403
Bme18I GGWCC 4 cut(s) 455, 1186, 1565, 2435
BmeRI GACNNNNNGTC 1 cut(s) 1155
BmeT110I CYCGRG 2 cut(s) 130, 562
BmgT120I GGNCC 7 cut(s) 118, 455, 566, 942, 1186, 1565, 2435
BmiI GGNNCC 2 cut(s) 567, 2226
BmrFI CCNGG 9 cut(s) 111, 477, 563, 564, 570, 787, 941, 1230, 1403
BmsI GCATC 9 cut(s) 487, 1267, 1289, 1356, 1432, 1511, 1790, 2097, 2280
BpmI CTGGAG 1 cut(s) 1667
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 2 cut(s) 37, 1839
BpuMI CCSGG 4 cut(s) 477, 563, 564, 941
BsaAI YACGTR 2 cut(s) 1245, 2030
BsaBI GATNNNNATC 1 cut(s) 2253
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 9 cut(s) 110, 270, 476, 562, 563, 569, 699, 786, 1120
BsaWI WCCGGW 2 cut(s) 1198, 2437
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 8 cut(s) 153, 256, 406, 569, 589, 914, 915, 2234
Bse1I ACTGG 5 cut(s) 1156, 1597, 1823, 2310, 2369
Bse8I GATNNNNATC 1 cut(s) 2253
BseAI TCCGGA 1 cut(s) 1198
BseBI CCWGG 5 cut(s) 111, 570, 787, 1230, 1403
BseDI CCNNGG 9 cut(s) 110, 270, 476, 562, 563, 569, 699, 786, 1120
BseGI GGATG 7 cut(s) 486, 968, 1110, 1160, 1398, 1743, 2271
BseJI GATNNNNATC 1 cut(s) 2253
BseLI CCNNNNNNNGG 8 cut(s) 153, 256, 406, 569, 589, 914, 915, 2234
BseMII CTCAG 3 cut(s) 185, 1196, 2037
BseNI ACTGG 5 cut(s) 1156, 1597, 1823, 2310, 2369
BseRI GAGGAG 1 cut(s) 1067
BseXI GCAGC 5 cut(s) 67, 89, 1501, 1819, 2318
BsgI GTGCAG 4 cut(s) 66, 96, 215, 2226
Bsh1236I CGCG 1 cut(s) 2160
BshFI GGCC 5 cut(s) 119, 475, 568, 939, 944
BsiHKCI CYCGRG 2 cut(s) 130, 562
BsiSI CCGG 7 cut(s) 180, 476, 563, 597, 940, 1199, 2438
BslFI GGGAC 3 cut(s) 1488, 2026, 2038
BslI CCNNNNNNNGG 8 cut(s) 153, 256, 406, 569, 589, 914, 915, 2234
BsmAI GTCTC 4 cut(s) 90, 148, 732, 1049
BsmFI GGGAC 3 cut(s) 1488, 2026, 2038
BsmI GAATGC 2 cut(s) 12, 1719
BsnI GGCC 5 cut(s) 119, 475, 568, 939, 944
Bso31I GGTCTC 1 cut(s) 148
BsoBI CYCGRG 2 cut(s) 130, 562
Bsp119I TTCGAA 1 cut(s) 1128
Bsp13I TCCGGA 1 cut(s) 1198
Bsp1407I TGTACA 1 cut(s) 2393
Bsp143I GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
BspACI CCGC 4 cut(s) 147, 212, 1253, 1316
BspANI GGCC 5 cut(s) 119, 475, 568, 939, 944
BspCNI CTCAG 3 cut(s) 186, 1195, 2038
BspEI TCCGGA 1 cut(s) 1198
BspFNI CGCG 1 cut(s) 2160
BspHI TCATGA 1 cut(s) 1419
BspLI GGNNCC 2 cut(s) 567, 2226
BspMI ACCTGC 3 cut(s) 237, 839, 1370
BspPI GGATC 5 cut(s) 639, 761, 1190, 2219, 2232
BspT104I TTCGAA 1 cut(s) 1128
BspTNI GGTCTC 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 2393
BsrI ACTGG 5 cut(s) 1156, 1597, 1823, 2310, 2369
BssECI CCNNGG 9 cut(s) 110, 270, 476, 562, 563, 569, 699, 786, 1120
BssMI GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
BssT1I CCWWGG 3 cut(s) 270, 699, 1120
Bst2UI CCWGG 5 cut(s) 111, 570, 787, 1230, 1403
Bst4CI ACNGT 8 cut(s) 87, 289, 524, 757, 857, 1112, 1829, 2422
Bst6I CTCTTC 1 cut(s) 1725
BstAUI TGTACA 1 cut(s) 2393
BstBAI YACGTR 2 cut(s) 1245, 2030
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 2 cut(s) 38, 1805
BstDEI CTNAG 6 cut(s) 194, 470, 506, 1182, 2046, 2120
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 7 cut(s) 486, 968, 1110, 1160, 1398, 1743, 2271
BstFNI CGCG 1 cut(s) 2160
BstH2I RGCGCY 3 cut(s) 263, 1006, 1334
BstHHI GCGC 4 cut(s) 262, 1005, 1333, 2162
BstKTI GATC 7 cut(s) 24, 634, 769, 782, 1198, 2158, 2227
BstMAI GTCTC 4 cut(s) 90, 148, 732, 1049
BstMBI GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
BstMWI GCNNNNNNNGC 1 cut(s) 1316
BstNI CCWGG 5 cut(s) 111, 570, 787, 1230, 1403
BstNSI RCATGY 2 cut(s) 1759, 2362
BstSCI CCNGG 9 cut(s) 109, 475, 561, 562, 568, 785, 939, 1228, 1401
BstSFI CTRYAG 3 cut(s) 883, 2089, 2421
BstSNI TACGTA 1 cut(s) 1245
BstUI CGCG 1 cut(s) 2160
BstV1I GCAGC 5 cut(s) 67, 89, 1501, 1819, 2318
BstX2I RGATCY 3 cut(s) 631, 1195, 2224
BstYI RGATCY 3 cut(s) 631, 1195, 2224
BsuRI GGCC 5 cut(s) 119, 475, 568, 939, 944
BtsCI GGATG 7 cut(s) 486, 968, 1110, 1160, 1398, 1743, 2271
BtsI GCAGTG 2 cut(s) 1614, 2363
BtsIMutI CAGTG 4 cut(s) 83, 753, 1614, 2363
BveI ACCTGC 3 cut(s) 237, 839, 1370
Cac8I GCNNGC 2 cut(s) 38, 1805
CciI TCATGA 1 cut(s) 1419
CfoI GCGC 4 cut(s) 262, 1005, 1333, 2162
Cfr13I GGNCC 7 cut(s) 118, 455, 566, 942, 1186, 1565, 2435
Cfr9I CCCGGG 1 cut(s) 562
CpoI CGGWCCG 1 cut(s) 2435
Csp6I GTAC 4 cut(s) 354, 1753, 2372, 2394
CspI CGGWCCG 1 cut(s) 2435
CviQI GTAC 4 cut(s) 354, 1753, 2372, 2394
DdeI CTNAG 6 cut(s) 194, 470, 506, 1182, 2046, 2120
DpnI GATC 7 cut(s) 23, 633, 768, 781, 1197, 2157, 2226
DpnII GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
DriI GACNNNNNGTC 1 cut(s) 1155
EaeI YGGCCR 1 cut(s) 937
Eam1104I CTCTTC 1 cut(s) 1725
Eam1105I GACNNNNNGTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 1725
Eco105I TACGTA 1 cut(s) 1245
Eco130I CCWWGG 3 cut(s) 270, 699, 1120
Eco31I GGTCTC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 2337
Eco47I GGWCC 4 cut(s) 455, 1186, 1565, 2435
Eco57I CTGAAG 1 cut(s) 730
Eco72I CACGTG 1 cut(s) 2030
Eco88I CYCGRG 2 cut(s) 130, 562
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 118, 1186
EcoRII CCWGG 5 cut(s) 109, 568, 785, 1228, 1401
EcoRV GATATC 1 cut(s) 2337
EcoT14I CCWWGG 3 cut(s) 270, 699, 1120
EcoT22I ATGCAT 3 cut(s) 1282, 1504, 2185
ErhI CCWWGG 3 cut(s) 270, 699, 1120
FalI AAGNNNNNCTT 2 cut(s) 1848, 1880
FaqI GGGAC 3 cut(s) 1488, 2026, 2038
FauNDI CATATG 1 cut(s) 727
FbaI TGATCA 1 cut(s) 21
FblI GTMKAC 3 cut(s) 705, 894, 2314
Fnu4HI GCNGC 6 cut(s) 78, 81, 1317, 1515, 1808, 2307
FokI GGATG 7 cut(s) 493, 955, 1117, 1167, 1385, 1750, 2258
Fsp4HI GCNGC 6 cut(s) 78, 81, 1317, 1515, 1808, 2307
FspBI CTAG 6 cut(s) 516, 645, 794, 1235, 1797, 2213
GlaI GCGC 4 cut(s) 261, 1004, 1332, 2161
GluI GCNGC 6 cut(s) 78, 81, 1317, 1515, 1808, 2307
GsuI CTGGAG 1 cut(s) 1667
HaeII RGCGCY 3 cut(s) 263, 1006, 1334
HaeIII GGCC 5 cut(s) 119, 475, 568, 939, 944
HapII CCGG 7 cut(s) 180, 476, 563, 597, 940, 1199, 2438
HhaI GCGC 4 cut(s) 262, 1005, 1333, 2162
Hin6I GCGC 4 cut(s) 260, 1003, 1331, 2160
HinP1I GCGC 4 cut(s) 260, 1003, 1331, 2160
HincII GTYRAC 4 cut(s) 7, 823, 895, 2315
HindII GTYRAC 4 cut(s) 7, 823, 895, 2315
HindIII AAGCTT 3 cut(s) 55, 445, 1768
HinfI GANTC 5 cut(s) 316, 383, 593, 1790, 2240
HpaI GTTAAC 2 cut(s) 7, 823
HpaII CCGG 7 cut(s) 180, 476, 563, 597, 940, 1199, 2438
HphI GGTGA 6 cut(s) 259, 776, 863, 1394, 1600, 1739
Hpy166II GTNNAC 8 cut(s) 7, 162, 642, 706, 823, 895, 1862, 2315
Hpy188I TCNGA 9 cut(s) 195, 247, 417, 555, 1149, 1550, 1960, 2083, 2476
Hpy188III TCNNGA 6 cut(s) 61, 1199, 1260, 1420, 1884, 1984
Hpy8I GTNNAC 8 cut(s) 7, 162, 642, 706, 823, 895, 1862, 2315
Hpy99I CGWCG 1 cut(s) 899
HpyAV CCTTC 4 cut(s) 261, 1547, 1838, 2195
HpyCH4III ACNGT 8 cut(s) 87, 289, 524, 757, 857, 1112, 1829, 2422
HpyCH4IV ACGT 7 cut(s) 164, 420, 1244, 1416, 1627, 2029, 2488
HpyF10VI GCNNNNNNNGC 1 cut(s) 1316
HpyF3I CTNAG 6 cut(s) 194, 470, 506, 1182, 2046, 2120
HpySE526I ACGT 7 cut(s) 164, 420, 1244, 1416, 1627, 2029, 2488
HspAI GCGC 4 cut(s) 260, 1003, 1331, 2160
Kpn2I TCCGGA 1 cut(s) 1198
Ksp22I TGATCA 1 cut(s) 21
KspAI GTTAAC 2 cut(s) 7, 823
Kzo9I GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
LmnI GCTCC 3 cut(s) 808, 1010, 1167
Lsp1109I GCAGC 5 cut(s) 67, 89, 1501, 1819, 2318
LweI GCATC 9 cut(s) 487, 1267, 1289, 1356, 1432, 1511, 1790, 2097, 2280
MaeI CTAG 6 cut(s) 516, 645, 794, 1235, 1797, 2213
MaeII ACGT 7 cut(s) 164, 420, 1244, 1416, 1627, 2029, 2488
MaeIII GTNAC 5 cut(s) 749, 851, 1112, 1588, 2361
MalI GATC 7 cut(s) 23, 633, 768, 781, 1197, 2157, 2226
MboI GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
MboII GAAGA 6 cut(s) 1205, 1275, 1316, 1742, 2026, 2142
MfeI CAATTG 2 cut(s) 608, 1311
MflI RGATCY 3 cut(s) 631, 1195, 2224
MmeI TCCRAC 2 cut(s) 1082, 2252
Mph1103I ATGCAT 3 cut(s) 1282, 1504, 2185
MroI TCCGGA 1 cut(s) 1198
MroXI GAANNNNTTC 1 cut(s) 2068
MseI TTAA 6 cut(s) 6, 105, 822, 1484, 1653, 2491
MslI CAYNNNNRTG 2 cut(s) 17, 1288
MspA1I CMGCKG 2 cut(s) 80, 1705
MspI CCGG 7 cut(s) 180, 476, 563, 597, 940, 1199, 2438
MspR9I CCNGG 9 cut(s) 111, 477, 563, 564, 570, 787, 941, 1230, 1403
MunI CAATTG 2 cut(s) 608, 1311
Mva1269I GAATGC 2 cut(s) 12, 1719
MvaI CCWGG 5 cut(s) 111, 570, 787, 1230, 1403
MvnI CGCG 1 cut(s) 2160
MwoI GCNNNNNNNGC 1 cut(s) 1316
NciI CCSGG 4 cut(s) 477, 563, 564, 941
NdeI CATATG 1 cut(s) 727
NdeII GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
NlaIV GGNNCC 2 cut(s) 567, 2226
NmuCI GTSAC 4 cut(s) 851, 1112, 1588, 2361
NsiI ATGCAT 3 cut(s) 1282, 1504, 2185
NspI RCATGY 2 cut(s) 1759, 2362
NspV TTCGAA 1 cut(s) 1128
OliI CACNNNNGTG 1 cut(s) 1288
PaeR7I CTCGAG 1 cut(s) 130
PagI TCATGA 1 cut(s) 1419
PaqCI CACCTGC 2 cut(s) 839, 1370
PctI GAATGC 2 cut(s) 12, 1719
PdmI GAANNNNTTC 1 cut(s) 2068
PfeI GAWTC 5 cut(s) 316, 383, 593, 1790, 2240
PflMI CCANNNNNTGG 1 cut(s) 2234
PfoI TCCNGGA 1 cut(s) 1401
PkrI GCNGC 6 cut(s) 79, 82, 1318, 1516, 1809, 2308
PmaCI CACGTG 1 cut(s) 2030
PmlI CACGTG 1 cut(s) 2030
Ppu21I YACGTR 2 cut(s) 1245, 2030
PpuMI RGGWCCY 1 cut(s) 1186
PsiI TTATAA 1 cut(s) 1575
Psp1406I AACGTT 2 cut(s) 420, 1627
Psp5II RGGWCCY 1 cut(s) 1186
Psp6I CCWGG 5 cut(s) 109, 568, 785, 1228, 1401
PspCI CACGTG 1 cut(s) 2030
PspGI CCWGG 5 cut(s) 109, 568, 785, 1228, 1401
PspN4I GGNNCC 2 cut(s) 567, 2226
PspPI GGNCC 7 cut(s) 118, 455, 566, 942, 1186, 1565, 2435
PspPPI RGGWCCY 1 cut(s) 1186
PspXI VCTCGAGB 1 cut(s) 130
PsuI RGATCY 3 cut(s) 631, 1195, 2224
PvuII CAGCTG 2 cut(s) 80, 1705
RsaI GTAC 4 cut(s) 355, 1754, 2373, 2395
RsaNI GTAC 4 cut(s) 354, 1753, 2372, 2394
RseI CAYNNNNRTG 2 cut(s) 17, 1288
Rsr2I CGGWCCG 1 cut(s) 2435
RsrII CGGWCCG 1 cut(s) 2435
SalI GTCGAC 2 cut(s) 893, 2313
SaqAI TTAA 6 cut(s) 6, 105, 822, 1484, 1653, 2491
SatI GCNGC 6 cut(s) 78, 81, 1317, 1515, 1808, 2307
Sau3AI GATC 7 cut(s) 21, 631, 766, 779, 1195, 2155, 2224
Sau96I GGNCC 7 cut(s) 118, 455, 566, 942, 1186, 1565, 2435
ScrFI CCNGG 9 cut(s) 111, 477, 563, 564, 570, 787, 941, 1230, 1403
SfaNI GCATC 9 cut(s) 487, 1267, 1289, 1356, 1432, 1511, 1790, 2097, 2280
SfcI CTRYAG 3 cut(s) 883, 2089, 2421
Sfr274I CTCGAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 1128
SinI GGWCC 4 cut(s) 455, 1186, 1565, 2435
SlaI CTCGAG 1 cut(s) 130
SmaI CCCGGG 1 cut(s) 564
SmiMI CAYNNNNRTG 2 cut(s) 17, 1288
SmlI CTYRAG 3 cut(s) 52, 130, 1854
SmoI CTYRAG 3 cut(s) 52, 130, 1854
SnaBI TACGTA 1 cut(s) 1245
SpeI ACTAGT 1 cut(s) 1234
SsiI CCGC 4 cut(s) 147, 212, 1253, 1316
SspI AATATT 1 cut(s) 832
SspMI CTAG 6 cut(s) 516, 645, 794, 1235, 1797, 2213
StyD4I CCNGG 9 cut(s) 109, 475, 561, 562, 568, 785, 939, 1228, 1401
StyI CCWWGG 3 cut(s) 270, 699, 1120
TaaI ACNGT 8 cut(s) 87, 289, 524, 757, 857, 1112, 1829, 2422
TaiI ACGT 7 cut(s) 167, 423, 1247, 1419, 1630, 2032, 2491
TaqI TCGA 9 cut(s) 131, 239, 530, 894, 1069, 1128, 1159, 1261, 2314
TatI WGTACW 2 cut(s) 1752, 2393
TauI GCSGC 1 cut(s) 1319
TfiI GAWTC 5 cut(s) 316, 383, 593, 1790, 2240
Tru1I TTAA 6 cut(s) 6, 105, 822, 1484, 1653, 2491
Tru9I TTAA 6 cut(s) 6, 105, 822, 1484, 1653, 2491
TscAI CASTG 4 cut(s) 90, 760, 1614, 2370
TseFI GTSAC 4 cut(s) 851, 1112, 1588, 2361
TseI GCWGC 5 cut(s) 77, 80, 1514, 1807, 2306
Tsp45I GTSAC 4 cut(s) 851, 1112, 1588, 2361
TspGWI ACGGA 3 cut(s) 423, 677, 2448
TspMI CCCGGG 1 cut(s) 562
TspRI CASTG 4 cut(s) 90, 760, 1614, 2370
Van91I CCANNNNNTGG 1 cut(s) 2234
VpaK11BI GGWCC 4 cut(s) 455, 1186, 1565, 2435
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 4 cut(s) 1060, 1130, 1953, 2066
XceI RCATGY 2 cut(s) 1759, 2362
XcmI CCANNNNNNNNNTGG 2 cut(s) 364, 932
XhoI CTCGAG 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 562
XmiI GTMKAC 3 cut(s) 705, 894, 2314
XmnI GAANNNNTTC 1 cut(s) 2068
XspI CTAG 6 cut(s) 516, 645, 794, 1235, 1797, 2213
Zsp2I ATGCAT 3 cut(s) 1282, 1504, 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.