RchiOBHm_Chr5g0025581

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000096
Physical Location & Seq
Forward (+)
19517172 .. 19520190
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30522

Sequence Viewer

Length: 2385 bp
ATGGTTAACAGCATTCCAATGATCACCTTATTGTGTTTGCTCGTCAGCTTCTGGACTTGTCATGGTGCAGCTGCACTGTTCATTGGAGACACGCTTAATCCAGGGGAGGCCCTCAATTCCTCGAGCTCTATGGTCTCCGCTTCGGGGGAGTTTACGTTGGGTTTCAACTCCGGCTACCTCATTCTGAGGTGCAAGAACTATGCGGTTTGGGTTGCCAACCTGCACACTCCGATTTCAGACCCTTCGTCGGCGCTTCTCACCTTGGAAAAGAACACAACTGTCCTGCAAATTACCCTGAAAGGCAAGAATCCCATCGTTCTTTACTCCCCTTTTCATGATAATAGTACCAATAATGTTGTGGCTACCCTGTTAGATTCTGGCAATTTAGTCCTACGAGAGGTCAATCAGAACGTTTCAAACTCTCCGTTGTGGCAAAGCTTTGACGGTCCTAATGTGGCTCTTAGGCCGGGGATGAAATTAGGTTTTGATGCTGGTCTAAGTATAACTAGCAACAGTATGTCGATTTGGAGTTGGCAGGAAAATCAGAACAGCCCGGGGCCAGGGTCTTTCAGCCTTTATTGGGATTCCGGCACGCGCCAATTGCAAGTTAGGAGAAACGGGGATCTTTGGTGGACTAGCGAAACATTGAAAAGTGGAATATACGAGTATGCTAATTTCCGTAATAAATCCAAGGTCTACAATTTTAGCGTGGTTTCATATGAGAATGGAGACTTCTTCAGTTACACTGTCGTTGGTGATCCAAATGGCAGATCAGCCTGGGTGCTAGACACAGGTGGGGAGCTTTATGATGTTAACTATAATATTTCTATTGGGTCAGCAGGTGACTGTGATGGCTATAATTATCACACCACCTATAGCTATGTCGCGATCGACGGCTATAACCCCGTTGCTGGGAAGTGCCAGAGATGGAGTGGCCGGGCCACTCTTTATTGTCCTGTGGCAACATCCTCTGGTGCATTTATTCAGCCGACAGAAAGGCGCTCCTTTAGACCAATCAGTATGAACATGTATATCAATTCAACATGGGAGGAGACAAATTCAAGTCGATATGGGATTTATGAATGTATGGCTACTTGTTGGATGGACTGTGACTGCCTTGGTTTCGAATTTCTCTTTGATAATCAGACTGGATGTCGATTTTGGAGCGTAAAGTATTCTCAGGTCCTTGAAGATCCGGATAGTTCTGCAACTAAATATATTATACCAGGACTAGTCGCTACGAATAAACCGCAATCTCGAAGAAACATAATTGGTGAGAACCAAATAAACATCCAGGATATGCTGAACGTCATGAAATCTAATAGACCTACTGATGCTAATGGGCTTCAAAATGATGGAAAGAGGGGACAAGATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACTTGCAACTTCTCTAATGAAAATAAGCTCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGGAAATTGGTGACTGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAACGTTCAGGGCAGGGATTATATGAGTTTAAGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGCTGTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAGAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAGCAGTTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAGTATTCCAGAATGAAAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACGAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACACGTGGATATATGTCCCCTGAGTATATTATGGGGGGAAAATTTTCCATAAAATCTGATGTCTATAGTTTCGGTGTATTGATGCTTGAAATCCTAAGTGGTAGAAGAAACAACAGCTTCTACAATGATGATCGCGCCCTCAATTTAGTTGGTTATGCATGGGCGATATGGAAAGAAGGTGCAGGACTAGAACTAATGGATCCAACACTTGGCGATTCATGTGATAAAGATCATTTGTTGAGGTGCATCCATGTTGGTTTGCTTTGTGTAGAGGAAAATGCAGCCAGTCGACCAAAGGTGTTAGATGTGATATCTATGCTGACTAATGAAAGCATGTCACTGCCAGTACCTACAAAACCAGCATTTTGTACAGAAAGAAATGTCATCACAACTACTATAGGTGGAAACGGACCGGAAGTGGTGGTATCGGTAAATGGTATCTCCAATTCTGATTTTGATGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

794

Amino Acids

88.56

Weight (kDa)

5.5

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 178 1.9e-18 D-mannose binding lectin
Pkinase PF00069 475 - 675 2.7e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 477 - 675 1.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1467
AarI CACCTGC 1 cut(s) 830
Acc36I ACCTGC 2 cut(s) 228, 830
AccB7I CCANNNNNTGG 1 cut(s) 2126
AccI GTMKAC 2 cut(s) 696, 2206
AccII CGCG 3 cut(s) 595, 887, 2052
AccIII TCCGGA 1 cut(s) 1195
AciI CCGC 3 cut(s) 138, 203, 1250
AclI AACGTT 2 cut(s) 411, 1519
AclWI GGATC 5 cut(s) 630, 752, 1187, 2111, 2124
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 4 cut(s) 1057, 1127, 1845, 1958
AcuI CTGAAG 1 cut(s) 721
AcvI CACGTG 1 cut(s) 1922
AfaI GTAC 4 cut(s) 346, 1646, 2265, 2287
AfiI CCNNNNNNNGG 8 cut(s) 144, 247, 397, 560, 580, 911, 912, 2126
AflIII ACRYGT 2 cut(s) 1026, 1919
AhdI GACNNNNNGTC 2 cut(s) 244, 1152
AhlI ACTAGT 1 cut(s) 1231
AjnI CCWGG 5 cut(s) 100, 559, 776, 1225, 1293
AjuI GAANNNNNNNTTGG 2 cut(s) 1437, 1469
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 4 cut(s) 81, 139, 723, 1046
AlwI GGATC 5 cut(s) 630, 752, 1187, 2111, 2124
AlwNI CAGNNNCTG 1 cut(s) 51
Ama87I CYCGRG 2 cut(s) 121, 553
Aor13HI TCCGGA 1 cut(s) 1195
AoxI GGCC 5 cut(s) 108, 464, 557, 934, 939
ApeKI GCWGC 5 cut(s) 68, 71, 1406, 1699, 2198
ApoI RAATTY 4 cut(s) 1057, 1127, 1845, 1958
Asp700I GAANNNNTTC 1 cut(s) 1960
AspLEI GCGC 4 cut(s) 253, 597, 1002, 2054
AspS9I GGNCC 7 cut(s) 109, 446, 557, 939, 1183, 1457, 2327
AsuC2I CCSGG 4 cut(s) 468, 554, 555, 938
AsuHPI GGTGA 7 cut(s) 16, 250, 767, 854, 1286, 1492, 1631
AsuII TTCGAA 1 cut(s) 1125
AvaI CYCGRG 2 cut(s) 121, 553
AvaII GGWCC 4 cut(s) 446, 1183, 1457, 2327
BamHI GGATCC 1 cut(s) 2116
BanII GRGCYC 1 cut(s) 128
BbrPI CACGTG 1 cut(s) 1922
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 5 cut(s) 58, 80, 1393, 1711, 2210
BccI CCATC 6 cut(s) 320, 845, 921, 1096, 1349, 2369
BceAI ACGGC 1 cut(s) 910
BciT130I CCWGG 5 cut(s) 102, 561, 778, 1227, 1295
BclI TGATCA 1 cut(s) 21
BcnI CCSGG 4 cut(s) 468, 554, 555, 938
BcoDI GTCTC 4 cut(s) 81, 139, 723, 1046
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 6 cut(s) 507, 636, 785, 1232, 1689, 2105
BfmI CTRYAG 3 cut(s) 874, 1981, 2313
BfoI RGCGCY 2 cut(s) 254, 1003
BfuAI ACCTGC 2 cut(s) 228, 830
BisI GCNGC 5 cut(s) 69, 72, 1407, 1700, 2199
BlsI GCNGC 5 cut(s) 70, 73, 1408, 1701, 2200
Bme1390I CCNGG 9 cut(s) 102, 468, 554, 555, 561, 778, 938, 1227, 1295
Bme18I GGWCC 4 cut(s) 446, 1183, 1457, 2327
BmeRI GACNNNNNGTC 2 cut(s) 244, 1152
BmeT110I CYCGRG 2 cut(s) 121, 553
BmgT120I GGNCC 7 cut(s) 109, 446, 557, 939, 1183, 1457, 2327
BmiI GGNNCC 2 cut(s) 558, 2118
BmrFI CCNGG 9 cut(s) 102, 468, 554, 555, 561, 778, 938, 1227, 1295
BmsI GCATC 6 cut(s) 478, 1324, 1403, 1682, 1989, 2172
BpmI CTGGAG 1 cut(s) 1559
Bpu14I TTCGAA 1 cut(s) 1125
BpuEI CTTGAG 1 cut(s) 1731
BpuMI CCSGG 4 cut(s) 468, 554, 555, 938
BsaAI YACGTR 1 cut(s) 1922
BsaBI GATNNNNATC 1 cut(s) 2145
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 9 cut(s) 101, 261, 467, 553, 554, 560, 690, 777, 1117
BsaWI WCCGGW 2 cut(s) 1195, 2329
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 8 cut(s) 144, 247, 397, 560, 580, 911, 912, 2126
Bse1I ACTGG 5 cut(s) 1153, 1489, 1715, 2202, 2261
Bse8I GATNNNNATC 1 cut(s) 2145
BseAI TCCGGA 1 cut(s) 1195
BseBI CCWGG 5 cut(s) 102, 561, 778, 1227, 1295
BseDI CCNNGG 9 cut(s) 101, 261, 467, 553, 554, 560, 690, 777, 1117
BseGI GGATG 7 cut(s) 477, 965, 1107, 1157, 1290, 1635, 2163
BseJI GATNNNNATC 1 cut(s) 2145
BseLI CCNNNNNNNGG 8 cut(s) 144, 247, 397, 560, 580, 911, 912, 2126
BseMII CTCAG 3 cut(s) 176, 1193, 1929
BseNI ACTGG 5 cut(s) 1153, 1489, 1715, 2202, 2261
BseRI GAGGAG 1 cut(s) 1064
BseXI GCAGC 5 cut(s) 58, 80, 1393, 1711, 2210
BseYI CCCAGC 1 cut(s) 911
BsgI GTGCAG 4 cut(s) 57, 87, 206, 2118
Bsh1236I CGCG 3 cut(s) 595, 887, 2052
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 5 cut(s) 110, 466, 559, 936, 941
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 2 cut(s) 121, 553
BsiSI CCGG 7 cut(s) 171, 467, 554, 588, 937, 1196, 2330
BslFI GGGAC 3 cut(s) 1380, 1918, 1930
BslI CCNNNNNNNGG 8 cut(s) 144, 247, 397, 560, 580, 911, 912, 2126
BsmAI GTCTC 4 cut(s) 81, 139, 723, 1046
BsmFI GGGAC 3 cut(s) 1380, 1918, 1930
BsmI GAATGC 2 cut(s) 12, 1611
BsnI GGCC 5 cut(s) 110, 466, 559, 936, 941
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 2 cut(s) 121, 553
Bsp119I TTCGAA 1 cut(s) 1125
Bsp1286I GDGCHC 1 cut(s) 128
Bsp13I TCCGGA 1 cut(s) 1195
Bsp1407I TGTACA 1 cut(s) 2285
Bsp143I GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
Bsp68I TCGCGA 1 cut(s) 887
BspACI CCGC 3 cut(s) 138, 203, 1250
BspANI GGCC 5 cut(s) 110, 466, 559, 936, 941
BspCNI CTCAG 3 cut(s) 177, 1192, 1930
BspEI TCCGGA 1 cut(s) 1195
BspFNI CGCG 3 cut(s) 595, 887, 2052
BspHI TCATGA 2 cut(s) 334, 1311
BspLI GGNNCC 2 cut(s) 558, 2118
BspMI ACCTGC 2 cut(s) 228, 830
BspPI GGATC 5 cut(s) 630, 752, 1187, 2111, 2124
BspT104I TTCGAA 1 cut(s) 1125
BspTNI GGTCTC 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 2285
BsrI ACTGG 5 cut(s) 1153, 1489, 1715, 2202, 2261
BssECI CCNNGG 9 cut(s) 101, 261, 467, 553, 554, 560, 690, 777, 1117
BssMI GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
BssT1I CCWWGG 3 cut(s) 261, 690, 1117
Bst2UI CCWGG 5 cut(s) 102, 561, 778, 1227, 1295
Bst4CI ACNGT 8 cut(s) 78, 280, 446, 515, 748, 848, 1109, 1721
Bst6I CTCTTC 1 cut(s) 1617
BstAUI TGTACA 1 cut(s) 2285
BstBAI YACGTR 1 cut(s) 1922
BstBI TTCGAA 1 cut(s) 1125
BstC8I GCNNGC 2 cut(s) 593, 1697
BstDEI CTNAG 6 cut(s) 185, 461, 497, 1179, 1938, 2012
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 7 cut(s) 477, 965, 1107, 1157, 1290, 1635, 2163
BstFNI CGCG 3 cut(s) 595, 887, 2052
BstH2I RGCGCY 2 cut(s) 254, 1003
BstHHI GCGC 4 cut(s) 253, 597, 1002, 2054
BstKTI GATC 9 cut(s) 24, 625, 760, 773, 891, 1195, 2050, 2119, 2149
BstMAI GTCTC 4 cut(s) 81, 139, 723, 1046
BstMBI GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
BstMCI CGRYCG 1 cut(s) 891
BstMWI GCNNNNNNNGC 1 cut(s) 601
BstNI CCWGG 5 cut(s) 102, 561, 778, 1227, 1295
BstNSI RCATGY 3 cut(s) 1030, 1651, 2254
BstSCI CCNGG 9 cut(s) 100, 466, 552, 553, 559, 776, 936, 1225, 1293
BstSFI CTRYAG 3 cut(s) 874, 1981, 2313
BstUI CGCG 3 cut(s) 595, 887, 2052
BstV1I GCAGC 5 cut(s) 58, 80, 1393, 1711, 2210
BstX2I RGATCY 3 cut(s) 622, 1192, 2116
BstYI RGATCY 3 cut(s) 622, 1192, 2116
BsuRI GGCC 5 cut(s) 110, 466, 559, 936, 941
BtsCI GGATG 7 cut(s) 477, 965, 1107, 1157, 1290, 1635, 2163
BtsI GCAGTG 2 cut(s) 1506, 2255
BtsIMutI CAGTG 4 cut(s) 74, 744, 1506, 2255
BtuMI TCGCGA 1 cut(s) 887
BveI ACCTGC 2 cut(s) 228, 830
Cac8I GCNNGC 2 cut(s) 593, 1697
CaiI CAGNNNCTG 1 cut(s) 51
CciI TCATGA 2 cut(s) 334, 1311
CfoI GCGC 4 cut(s) 253, 597, 1002, 2054
Cfr13I GGNCC 7 cut(s) 109, 446, 557, 939, 1183, 1457, 2327
Cfr9I CCCGGG 1 cut(s) 553
CpoI CGGWCCG 1 cut(s) 2327
Csp6I GTAC 4 cut(s) 345, 1645, 2264, 2286
CspI CGGWCCG 1 cut(s) 2327
CviQI GTAC 4 cut(s) 345, 1645, 2264, 2286
DdeI CTNAG 6 cut(s) 185, 461, 497, 1179, 1938, 2012
DpnI GATC 9 cut(s) 23, 624, 759, 772, 890, 1194, 2049, 2118, 2148
DpnII GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
DriI GACNNNNNGTC 2 cut(s) 244, 1152
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 1 cut(s) 1617
Eam1105I GACNNNNNGTC 2 cut(s) 244, 1152
EarI CTCTTC 1 cut(s) 1617
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 3 cut(s) 261, 690, 1117
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 2229
Eco47I GGWCC 4 cut(s) 446, 1183, 1457, 2327
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 721
Eco72I CACGTG 1 cut(s) 1922
Eco88I CYCGRG 2 cut(s) 121, 553
EcoICRI GAGCTC 1 cut(s) 126
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoO109I RGGNCCY 2 cut(s) 109, 1183
EcoRII CCWGG 5 cut(s) 100, 559, 776, 1225, 1293
EcoRV GATATC 1 cut(s) 2229
EcoT14I CCWWGG 3 cut(s) 261, 690, 1117
EcoT22I ATGCAT 2 cut(s) 1396, 2077
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 3 cut(s) 261, 690, 1117
FalI AAGNNNNNCTT 2 cut(s) 1740, 1772
FaqI GGGAC 3 cut(s) 1380, 1918, 1930
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 1 cut(s) 21
FblI GTMKAC 2 cut(s) 696, 2206
Fnu4HI GCNGC 5 cut(s) 69, 72, 1407, 1700, 2199
FokI GGATG 7 cut(s) 484, 952, 1114, 1164, 1277, 1642, 2150
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 5 cut(s) 69, 72, 1407, 1700, 2199
FspBI CTAG 6 cut(s) 507, 636, 785, 1232, 1689, 2105
GlaI GCGC 4 cut(s) 252, 596, 1001, 2053
GluI GCNGC 5 cut(s) 69, 72, 1407, 1700, 2199
GsaI CCCAGC 1 cut(s) 915
GsuI CTGGAG 1 cut(s) 1559
HaeII RGCGCY 2 cut(s) 254, 1003
HaeIII GGCC 5 cut(s) 110, 466, 559, 936, 941
HapII CCGG 7 cut(s) 171, 467, 554, 588, 937, 1196, 2330
HhaI GCGC 4 cut(s) 253, 597, 1002, 2054
Hin6I GCGC 4 cut(s) 251, 595, 1000, 2052
HinP1I GCGC 4 cut(s) 251, 595, 1000, 2052
HincII GTYRAC 3 cut(s) 7, 814, 2207
HindII GTYRAC 3 cut(s) 7, 814, 2207
HindIII AAGCTT 2 cut(s) 436, 1660
HinfI GANTC 5 cut(s) 307, 374, 584, 1682, 2132
HpaI GTTAAC 2 cut(s) 7, 814
HpaII CCGG 7 cut(s) 171, 467, 554, 588, 937, 1196, 2330
HphI GGTGA 7 cut(s) 16, 250, 767, 854, 1286, 1492, 1631
Hpy166II GTNNAC 7 cut(s) 7, 153, 633, 697, 814, 1754, 2207
Hpy188III TCNNGA 8 cut(s) 52, 335, 886, 1196, 1257, 1312, 1776, 1876
Hpy8I GTNNAC 7 cut(s) 7, 153, 633, 697, 814, 1754, 2207
Hpy99I CGWCG 2 cut(s) 250, 896
HpyAV CCTTC 4 cut(s) 252, 1439, 1730, 2087
HpyCH4III ACNGT 8 cut(s) 78, 280, 446, 515, 748, 848, 1109, 1721
HpyCH4IV ACGT 6 cut(s) 155, 411, 1308, 1519, 1921, 2380
HpyF10VI GCNNNNNNNGC 1 cut(s) 601
HpyF3I CTNAG 6 cut(s) 185, 461, 497, 1179, 1938, 2012
HpySE526I ACGT 6 cut(s) 155, 411, 1308, 1519, 1921, 2380
HspAI GCGC 4 cut(s) 251, 595, 1000, 2052
Kpn2I TCCGGA 1 cut(s) 1195
Ksp22I TGATCA 1 cut(s) 21
KspAI GTTAAC 2 cut(s) 7, 814
Kzo9I GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
LmnI GCTCC 3 cut(s) 799, 1007, 1164
Lsp1109I GCAGC 5 cut(s) 58, 80, 1393, 1711, 2210
LweI GCATC 6 cut(s) 478, 1324, 1403, 1682, 1989, 2172
MaeI CTAG 6 cut(s) 507, 636, 785, 1232, 1689, 2105
MaeII ACGT 6 cut(s) 155, 411, 1308, 1519, 1921, 2380
MaeIII GTNAC 5 cut(s) 740, 842, 1109, 1480, 2253
MalI GATC 9 cut(s) 23, 624, 759, 772, 890, 1194, 2049, 2118, 2148
MboI GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
MboII GAAGA 6 cut(s) 727, 1202, 1272, 1634, 1918, 2034
MfeI CAATTG 1 cut(s) 599
MflI RGATCY 3 cut(s) 622, 1192, 2116
MhlI GDGCHC 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 1079, 2144
Mph1103I ATGCAT 2 cut(s) 1396, 2077
MroI TCCGGA 1 cut(s) 1195
MroXI GAANNNNTTC 1 cut(s) 1960
MseI TTAA 6 cut(s) 6, 96, 813, 1376, 1545, 2383
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 71, 1597
MspI CCGG 7 cut(s) 171, 467, 554, 588, 937, 1196, 2330
MspR9I CCNGG 9 cut(s) 102, 468, 554, 555, 561, 778, 938, 1227, 1295
MunI CAATTG 1 cut(s) 599
Mva1269I GAATGC 2 cut(s) 12, 1611
MvaI CCWGG 5 cut(s) 102, 561, 778, 1227, 1295
MvnI CGCG 3 cut(s) 595, 887, 2052
MwoI GCNNNNNNNGC 1 cut(s) 601
NciI CCSGG 4 cut(s) 468, 554, 555, 938
NdeI CATATG 1 cut(s) 718
NdeII GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
NlaIV GGNNCC 2 cut(s) 558, 2118
NmuCI GTSAC 4 cut(s) 842, 1109, 1480, 2253
NruI TCGCGA 1 cut(s) 887
NsiI ATGCAT 2 cut(s) 1396, 2077
NspI RCATGY 3 cut(s) 1030, 1651, 2254
NspV TTCGAA 1 cut(s) 1125
PaeR7I CTCGAG 1 cut(s) 121
PagI TCATGA 2 cut(s) 334, 1311
PaqCI CACCTGC 1 cut(s) 830
PciI ACATGT 1 cut(s) 1026
PctI GAATGC 2 cut(s) 12, 1611
PdmI GAANNNNTTC 1 cut(s) 1960
PfeI GAWTC 5 cut(s) 307, 374, 584, 1682, 2132
PflMI CCANNNNNTGG 1 cut(s) 2126
PfoI TCCNGGA 1 cut(s) 1293
PkrI GCNGC 5 cut(s) 70, 73, 1408, 1701, 2200
Ple19I CGATCG 1 cut(s) 891
PmaCI CACGTG 1 cut(s) 1922
PmlI CACGTG 1 cut(s) 1922
Ppu21I YACGTR 1 cut(s) 1922
PpuMI RGGWCCY 1 cut(s) 1183
PscI ACATGT 1 cut(s) 1026
PsiI TTATAA 1 cut(s) 1467
Psp124BI GAGCTC 1 cut(s) 128
Psp1406I AACGTT 2 cut(s) 411, 1519
Psp5II RGGWCCY 1 cut(s) 1183
Psp6I CCWGG 5 cut(s) 100, 559, 776, 1225, 1293
PspCI CACGTG 1 cut(s) 1922
PspFI CCCAGC 1 cut(s) 911
PspGI CCWGG 5 cut(s) 100, 559, 776, 1225, 1293
PspN4I GGNNCC 2 cut(s) 558, 2118
PspPI GGNCC 7 cut(s) 109, 446, 557, 939, 1183, 1457, 2327
PspPPI RGGWCCY 1 cut(s) 1183
PspXI VCTCGAGB 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 3 cut(s) 622, 1192, 2116
PvuI CGATCG 1 cut(s) 891
PvuII CAGCTG 2 cut(s) 71, 1597
RruI TCGCGA 1 cut(s) 887
RsaI GTAC 4 cut(s) 346, 1646, 2265, 2287
RsaNI GTAC 4 cut(s) 345, 1645, 2264, 2286
RseI CAYNNNNRTG 1 cut(s) 17
Rsr2I CGGWCCG 1 cut(s) 2327
RsrII CGGWCCG 1 cut(s) 2327
SacI GAGCTC 1 cut(s) 128
SalI GTCGAC 1 cut(s) 2205
SaqAI TTAA 6 cut(s) 6, 96, 813, 1376, 1545, 2383
SatI GCNGC 5 cut(s) 69, 72, 1407, 1700, 2199
Sau3AI GATC 9 cut(s) 21, 622, 757, 770, 888, 1192, 2047, 2116, 2146
Sau96I GGNCC 7 cut(s) 109, 446, 557, 939, 1183, 1457, 2327
ScrFI CCNGG 9 cut(s) 102, 468, 554, 555, 561, 778, 938, 1227, 1295
SduI GDGCHC 1 cut(s) 128
SfaNI GCATC 6 cut(s) 478, 1324, 1403, 1682, 1989, 2172
SfcI CTRYAG 3 cut(s) 874, 1981, 2313
Sfr274I CTCGAG 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 1125
SinI GGWCC 4 cut(s) 446, 1183, 1457, 2327
SlaI CTCGAG 1 cut(s) 121
SmaI CCCGGG 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 2 cut(s) 121, 1746
SmoI CTYRAG 2 cut(s) 121, 1746
SpeI ACTAGT 1 cut(s) 1231
SsiI CCGC 3 cut(s) 138, 203, 1250
SspI AATATT 1 cut(s) 823
SspMI CTAG 6 cut(s) 507, 636, 785, 1232, 1689, 2105
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 9 cut(s) 100, 466, 552, 553, 559, 776, 936, 1225, 1293
StyI CCWWGG 3 cut(s) 261, 690, 1117
TaaI ACNGT 8 cut(s) 78, 280, 446, 515, 748, 848, 1109, 1721
TaiI ACGT 6 cut(s) 158, 414, 1311, 1522, 1924, 2383
TaqI TCGA 8 cut(s) 122, 521, 891, 1066, 1125, 1156, 1258, 2206
TatI WGTACW 2 cut(s) 1644, 2285
TfiI GAWTC 5 cut(s) 307, 374, 584, 1682, 2132
Tru1I TTAA 6 cut(s) 6, 96, 813, 1376, 1545, 2383
Tru9I TTAA 6 cut(s) 6, 96, 813, 1376, 1545, 2383
TscAI CASTG 4 cut(s) 81, 751, 1506, 2262
TseFI GTSAC 4 cut(s) 842, 1109, 1480, 2253
TseI GCWGC 5 cut(s) 68, 71, 1406, 1699, 2198
Tsp45I GTSAC 4 cut(s) 842, 1109, 1480, 2253
TspGWI ACGGA 3 cut(s) 414, 668, 2340
TspMI CCCGGG 1 cut(s) 553
TspRI CASTG 4 cut(s) 81, 751, 1506, 2262
Van91I CCANNNNNTGG 1 cut(s) 2126
VpaK11BI GGWCC 4 cut(s) 446, 1183, 1457, 2327
XagI CCTNNNNNAGG 1 cut(s) 395
XapI RAATTY 4 cut(s) 1057, 1127, 1845, 1958
XceI RCATGY 3 cut(s) 1030, 1651, 2254
XcmI CCANNNNNNNNNTGG 2 cut(s) 355, 929
XhoI CTCGAG 1 cut(s) 121
XmaI CCCGGG 1 cut(s) 553
XmiI GTMKAC 2 cut(s) 696, 2206
XmnI GAANNNNTTC 1 cut(s) 1960
XspI CTAG 6 cut(s) 507, 636, 785, 1232, 1689, 2105
Zsp2I ATGCAT 2 cut(s) 1396, 2077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.