MD10G1208000.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000096
Physical Location & Seq
Forward (+)
30753221 .. 30756495
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1208000.v1.1.491

Sequence Viewer

Length: 2364 bp
ATGGCTAGCTGCAGAACTTGCTTGCTCATATTTTCAAGCTTAGTGTGGATTTGTTATTATACAGCTGCAAGTGCACAGGACACACTCAAACCAGGTGACACTCTCAAACCAGGTGACACACTCAACTCTACAACTTATTTATGTTCTGCAATGGGGACCTTCTGCTTGGGTTTCTTTGTATATGACAAATCAAATTCCAGTCAGTTGTGCATACAGCGCGAAGGCAGTGGTAACCAGGGATGGATTGCCAGCCGAGACACACCTATTTTATACCCTTCAGGAGTTCTTACCTTCGACAAGAACAAAACGTTGAAAATTGTGGACCGAGGCGGGACGCGGTTGGAGCTTTACTCTGCCAGTATAGAAATAGCAAATTCAAACACTAGTACTGTGGTGGCTACTCTACTGGATTCTGGCAATTTTGTCGTACAAGAAGTGAACTCTATCCATGGATCGAAAAATCGGGTCTTGTGGCAAAGTTTTGATCATCCAATAGATACCCTTTATCCAGGCATGAAATTAGGGGTTAACCACAGAAACGGGCACATTTTGTCACTTACTTCATGGTCAAGTCAGTCCAATCCAGAGCCGGGGCCTTTCACCCTTGAATGGGACCACAAAACGCAAGAATTGCAGATTAAGCGACGCGGAGTGGTTTACTGGACTAGTGGAGCCTCTACAAGTAAGAGATTTAAGCAGCTGATGAGTAGGTATAATATTAGTATTGTTTCCAATGAAACTGAAGCCTCCTTCACTTACCACCCTCTAAACCAGACTTCAACTGAATGGGTGCTATCCTTAGCTGGACAACTAGACAACGCTGGAGAATTTGTGATTGCAAGAACAGAAAACTGTTATGGCTATAACACAGATGGAGGATGCCAGAGATGGGCGGAGAAGCCAACTTGCAGACATGTTGGTGACATATTCGAGCTAAGACGTGGTTACTTTACTCCAACCATCTCAAATAAAACCTACCCGATTGATCCTAATACAAGTCTAAGTCTTAGCGATTGTAAAGATTCTTGTTGGAAAAATTGCGAGTGCCTTGGATTTAATGTTCTAGATGAAGATGATCAATCTGGATGTCAATATTATACCAGAAATATCTCAGAGTTCCTTGAAGGCTCCACTGGTGATACTACTTTCAATATTTTGAAAACAAAGTCACCTCGCCATAATGGATGCATACAAAAACGCGGATATTTATTGGCACTAGTATCACAGCTGCTAAGAAAATGTGCACTTTCAGGCGAGAAAGAGACAAAGATCATTGAAGATGAATTGCTTGACTTAATGAAATCTGATCGACCTACTGATGCCAATGCACATCAAAATCATGGATATATGAGACCGGATTTAAGTGTCTTTAGTTATGCATCTGTAATGGCTGCCACATGTAATTTCTCTCCAGAAAACAAGCTCGGACAAGGGGGATTTGGTCCTGTTTATAAGGGAAAATTGGTGACGGGACAAGAAGTAGCTGTTAAGAGGCTTTCAAAATGTTCGGGGCAAGGAACACTGGAGTTTAAGAATGAATTGGTACTCATATATGAACTCCAACATAAAAACCTTGTCAAACTTTTTGGATTTTGCATTCATGGTGAAGAGAGGATGCTGATATACGAGTACATGCCAAACAAAAGTTTGGACTACTTTTTATTCGATTCAACAAGAGTCACGCCACTTGATTGGAAGAAGCGTTTTATTATAATTGAAGGAATTGCTCAAGGATTGCTTTACTTGCACAAGTACTCAAGAGTAACAGTAATACATAGAGATTTAAAAGCAAGTAACATACTACTTGACAAAAATATGAACCCCAAAATATCTGATTTTGGTATGGCAAGGATTTTCAAGCATAATGAACTTGAAGCAAATACTAATAGGGTTGTCGGAACATATGGTTACATGTCTCCTGAGTACGCCATGCAGGGCATCTTTTCCATAAAATCTGATGTTTATAGTTTTGGAGTGTTAATGCTTGAAATTGTAAGCGGCAGGAGAAACAATAGCTTCTACAATGCTGATCGTCTGCTTAATATAGTAGGATATGCATGGGAATTATGGAAGGAAGGCACAGTTCTAGAATTCATGGATCCAACATTAGGCGATTCATGCGTCAAAGATCAATTGTTAAGATGTGTTCATGTTGGTCTGCTATGCGTGGAAGAAAATGCAGCTGATCGGCCTACCATGCCAGATGTTGTATCTATGTTGACAAATCAAAGCACACCGATGCCATTACCTGCAAAGCCAGCATTTTTTACAGAAAGAAATGTTGTTGAAGCTGGTCTAAGTGGAAAAAAACCAGAAATTTTTTCCGCAAATGAGATATCCAATTCAACTGATGTACCGCGATGA

Protein Analysis

788

Amino Acids

88.33

Weight (kDa)

6.77

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 81 - 189 5.4e-23 D-mannose binding lectin
Pkinase PF00069 471 - 669 1.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 472 - 739 6.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1452, 1712
Acc36I ACCTGC 1 cut(s) 2257
AccII CGCG 5 cut(s) 219, 337, 648, 1200, 2359
AciI CCGC 8 cut(s) 330, 337, 648, 893, 1200, 1998, 2325, 2357
AclI AACGTT 1 cut(s) 308
AclWI GGATC 4 cut(s) 460, 980, 2093, 2106
AcsI RAATTY 5 cut(s) 193, 373, 827, 2090, 2316
AcuI CTGAAG 2 cut(s) 261, 762
AfaI GTAC 7 cut(s) 388, 429, 1545, 1631, 1754, 1925, 2355
AfiI CCNNNNNNNGG 5 cut(s) 590, 609, 610, 889, 2108
AflIII ACRYGT 3 cut(s) 913, 1397, 1911
AhlI ACTAGT 3 cut(s) 383, 665, 1216
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 4 cut(s) 91, 109, 234, 508
AloI GAACNNNNNNTCC 2 cut(s) 1044, 1076
Alw21I GWGCWC 2 cut(s) 76, 1246
Alw26I GTCTC 4 cut(s) 249, 1256, 1345, 1920
Alw44I GTGCAC 2 cut(s) 72, 1242
AlwI GGATC 4 cut(s) 460, 980, 2093, 2106
AoxI GGCC 2 cut(s) 593, 2189
ApaLI GTGCAC 2 cut(s) 72, 1242
ApeKI GCWGC 6 cut(s) 9, 65, 697, 1228, 1391, 2180
ApoI RAATTY 5 cut(s) 193, 373, 827, 2090, 2316
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 5 cut(s) 156, 322, 593, 613, 1442
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 8 cut(s) 107, 125, 592, 932, 1148, 1161, 1477, 1616
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 156, 322, 613, 1442
BaeGI GKGCMC 3 cut(s) 76, 546, 1246
BaeI ACNNNNGTAYC 1 cut(s) 2337
BamHI GGATCC 1 cut(s) 2098
Bbv12I GWGCWC 2 cut(s) 76, 1246
BbvI GCAGC 5 cut(s) 52, 709, 1215, 1378, 2192
BccI CCATC 4 cut(s) 234, 866, 882, 968
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 4 cut(s) 93, 111, 236, 510
BclI TGATCA 2 cut(s) 484, 1075
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 4 cut(s) 249, 1256, 1345, 1920
BcuI ACTAGT 3 cut(s) 383, 665, 1216
BfaI CTAG 7 cut(s) 6, 384, 666, 812, 1064, 1217, 2087
BfmI CTRYAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 2257
BisI GCNGC 7 cut(s) 10, 66, 698, 1229, 1392, 1999, 2181
BlsI GCNGC 7 cut(s) 11, 67, 699, 1230, 1393, 2000, 2182
BmcAI AGTACT 2 cut(s) 388, 1754
Bme1390I CCNGG 5 cut(s) 93, 111, 236, 510, 591
Bme18I GGWCC 4 cut(s) 156, 322, 613, 1442
BmgBI CACGTC 1 cut(s) 941
BmgT120I GGNCC 5 cut(s) 156, 322, 593, 613, 1442
BmiI GGNNCC 6 cut(s) 157, 594, 614, 673, 1129, 2100
BmrFI CCNGG 5 cut(s) 93, 111, 236, 510, 591
BmsI GCATC 7 cut(s) 869, 1175, 1309, 1388, 1605, 1947, 2229
BmtI GCTAGC 1 cut(s) 9
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 3 cut(s) 843, 1395, 1544
Bpu10I CCTNAGC 1 cut(s) 799
BpuEI CTTGAG 2 cut(s) 1713, 1741
BpuMI CCSGG 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 1345
BsaJI CCNNGG 5 cut(s) 235, 325, 448, 590, 1048
BsaWI WCCGGW 1 cut(s) 1354
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 5 cut(s) 590, 609, 610, 889, 2108
Bse1I ACTGG 6 cut(s) 198, 357, 411, 665, 1138, 1527
Bse3DI GCAATG 1 cut(s) 156
BseBI CCWGG 4 cut(s) 93, 111, 236, 510
BseDI CCNNGG 5 cut(s) 235, 325, 448, 590, 1048
BseGI GGATG 6 cut(s) 245, 487, 884, 1091, 1190, 1620
BseLI CCNNNNNNNGG 5 cut(s) 590, 609, 610, 889, 2108
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 1125, 1911
BseNI ACTGG 6 cut(s) 198, 357, 411, 665, 1138, 1527
BseSI GKGCMC 3 cut(s) 76, 546, 1246
BseXI GCAGC 5 cut(s) 52, 709, 1215, 1378, 2192
Bsh1236I CGCG 5 cut(s) 219, 337, 648, 1200, 2359
BshFI GGCC 2 cut(s) 595, 2191
BsiHKAI GWGCWC 2 cut(s) 76, 1246
BsiSI CCGG 2 cut(s) 590, 1355
BslFI GGGAC 4 cut(s) 169, 346, 626, 1485
BslI CCNNNNNNNGG 5 cut(s) 590, 609, 610, 889, 2108
BsmAI GTCTC 4 cut(s) 249, 1256, 1345, 1920
BsmFI GGGAC 4 cut(s) 169, 346, 626, 1485
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 2 cut(s) 595, 2191
Bso31I GGTCTC 1 cut(s) 1345
Bsp1286I GDGCHC 3 cut(s) 76, 546, 1246
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 8 cut(s) 330, 337, 648, 893, 1200, 1998, 2325, 2357
BspANI GGCC 2 cut(s) 595, 2191
BspCNI CTCAG 2 cut(s) 1124, 1912
BspFNI CGCG 5 cut(s) 219, 337, 648, 1200, 2359
BspLI GGNNCC 6 cut(s) 157, 594, 614, 673, 1129, 2100
BspMAI CTGCAG 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 2257
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 4 cut(s) 460, 980, 2093, 2106
BspTNI GGTCTC 1 cut(s) 1345
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 6 cut(s) 198, 357, 411, 665, 1138, 1527
BssECI CCNNGG 5 cut(s) 235, 325, 448, 590, 1048
BssT1I CCWWGG 2 cut(s) 448, 1048
Bst2UI CCWGG 4 cut(s) 93, 111, 236, 510
Bst4CI ACNGT 4 cut(s) 391, 854, 1768, 2083
Bst6I CTCTTC 1 cut(s) 1602
BstAPI GCANNNNNTGC 2 cut(s) 18, 631
BstC8I GCNNGC 4 cut(s) 7, 23, 250, 2259
BstDEI CTNAG 9 cut(s) 40, 799, 935, 1001, 1007, 1111, 1232, 1920, 2297
BstDSI CCRYGG 1 cut(s) 448
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 6 cut(s) 245, 487, 884, 1091, 1190, 1620
BstFNI CGCG 5 cut(s) 219, 337, 648, 1200, 2359
BstHHI GCGC 1 cut(s) 219
BstMAI GTCTC 4 cut(s) 249, 1256, 1345, 1920
BstNI CCWGG 4 cut(s) 93, 111, 236, 510
BstNSI RCATGY 4 cut(s) 917, 1401, 1636, 1915
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 5 cut(s) 91, 109, 234, 508, 589
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 3 cut(s) 76, 546, 1246
BstUI CGCG 5 cut(s) 219, 337, 648, 1200, 2359
BstV1I GCAGC 5 cut(s) 52, 709, 1215, 1378, 2192
BstX2I RGATCY 1 cut(s) 2098
BstXI CCANNNNNNTGG 1 cut(s) 1692
BstYI RGATCY 1 cut(s) 2098
BsuRI GGCC 2 cut(s) 595, 2191
BtgI CCRYGG 1 cut(s) 448
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 6 cut(s) 245, 487, 884, 1091, 1190, 1620
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 3 cut(s) 232, 1131, 1520
BveI ACCTGC 1 cut(s) 2257
Cac8I GCNNGC 4 cut(s) 7, 23, 250, 2259
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 5 cut(s) 156, 322, 593, 613, 1442
CseI GACGC 3 cut(s) 343, 654, 2110
CsiI ACCWGGT 2 cut(s) 91, 109
Csp6I GTAC 7 cut(s) 387, 428, 1544, 1630, 1753, 1924, 2354
CviQI GTAC 7 cut(s) 387, 428, 1544, 1630, 1753, 1924, 2354
DdeI CTNAG 9 cut(s) 40, 799, 935, 1001, 1007, 1111, 1232, 1920, 2297
DraI TTTAAA 1 cut(s) 1785
Eam1104I CTCTTC 1 cut(s) 1602
EarI CTCTTC 1 cut(s) 1602
EciI GGCGGA 1 cut(s) 908
Eco130I CCWWGG 2 cut(s) 448, 1048
Eco31I GGTCTC 1 cut(s) 1345
Eco32I GATATC 1 cut(s) 2337
Eco47I GGWCC 4 cut(s) 156, 322, 613, 1442
Eco57I CTGAAG 2 cut(s) 261, 762
Eco91I GGTNACC 1 cut(s) 230
EcoO109I RGGNCCY 2 cut(s) 156, 593
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 2090
EcoRII CCWGG 4 cut(s) 91, 109, 234, 508
EcoRV GATATC 1 cut(s) 2337
EcoT14I CCWWGG 2 cut(s) 448, 1048
EcoT22I ATGCAT 3 cut(s) 1190, 1381, 2059
ErhI CCWWGG 2 cut(s) 448, 1048
FalI AAGNNNNNCTT 2 cut(s) 1722, 1754
FaqI GGGAC 4 cut(s) 169, 346, 626, 1485
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 1903
FbaI TGATCA 2 cut(s) 484, 1075
Fnu4HI GCNGC 7 cut(s) 10, 66, 698, 1229, 1392, 1999, 2181
FokI GGATG 6 cut(s) 252, 474, 891, 1098, 1197, 1627
Fsp4HI GCNGC 7 cut(s) 10, 66, 698, 1229, 1392, 1999, 2181
FspBI CTAG 7 cut(s) 6, 384, 666, 812, 1064, 1217, 2087
GlaI GCGC 1 cut(s) 218
GluI GCNGC 7 cut(s) 10, 66, 698, 1229, 1392, 1999, 2181
GsuI CTGGAG 3 cut(s) 843, 1395, 1544
HaeIII GGCC 2 cut(s) 595, 2191
HapII CCGG 2 cut(s) 590, 1355
HgaI GACGC 3 cut(s) 343, 654, 2110
HhaI GCGC 1 cut(s) 219
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 529, 2220
HindII GTYRAC 2 cut(s) 529, 2220
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 5 cut(s) 410, 1022, 1667, 1677, 2114
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 2 cut(s) 590, 1355
HphI GGTGA 8 cut(s) 107, 125, 592, 932, 1148, 1161, 1477, 1616
Hpy166II GTNNAC 7 cut(s) 74, 322, 439, 529, 658, 1244, 2220
Hpy188I TCNGA 6 cut(s) 1114, 1306, 1427, 1834, 1898, 1957
Hpy188III TCNNGA 8 cut(s) 279, 584, 1064, 1083, 1412, 1758, 1919, 2087
Hpy8I GTNNAC 7 cut(s) 74, 322, 439, 529, 658, 1244, 2220
Hpy99I CGWCG 1 cut(s) 648
HpyAV CCTTC 9 cut(s) 169, 215, 285, 301, 760, 1118, 1712, 2065, 2069
HpyCH4III ACNGT 4 cut(s) 391, 854, 1768, 2083
HpyCH4IV ACGT 2 cut(s) 308, 940
HpyF3I CTNAG 9 cut(s) 40, 799, 935, 1001, 1007, 1111, 1232, 1920, 2297
HpySE526I ACGT 2 cut(s) 308, 940
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 2 cut(s) 484, 1075
KspAI GTTAAC 1 cut(s) 529
LmnI GCTCC 3 cut(s) 343, 671, 1133
Lsp1109I GCAGC 5 cut(s) 52, 709, 1215, 1378, 2192
LweI GCATC 7 cut(s) 869, 1175, 1309, 1388, 1605, 1947, 2229
MabI ACCWGGT 2 cut(s) 91, 109
MaeI CTAG 7 cut(s) 6, 384, 666, 812, 1064, 1217, 2087
MaeII ACGT 2 cut(s) 308, 940
MboII GAAGA 5 cut(s) 1082, 1289, 1619, 1708, 2183
MfeI CAATTG 1 cut(s) 2132
MflI RGATCY 1 cut(s) 2098
MhlI GDGCHC 3 cut(s) 76, 546, 1246
MlyI GAGTC 1 cut(s) 1686
MmeI TCCRAC 6 cut(s) 321, 980, 1010, 1585, 1876, 2126
MnlI CCTC 8 cut(s) 320, 685, 757, 774, 869, 1182, 1485, 1605
Mph1103I ATGCAT 3 cut(s) 1190, 1381, 2059
MslI CAYNNNNRTG 2 cut(s) 918, 2237
MspA1I CMGCKG 4 cut(s) 65, 700, 1228, 2183
MspI CCGG 2 cut(s) 590, 1355
MspR9I CCNGG 5 cut(s) 93, 111, 236, 510, 591
MunI CAATTG 1 cut(s) 2132
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 4 cut(s) 93, 111, 236, 510
MvnI CGCG 5 cut(s) 219, 337, 648, 1200, 2359
NciI CCSGG 1 cut(s) 591
NcoI CCATGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 1903
NheI GCTAGC 1 cut(s) 5
NlaIV GGNNCC 6 cut(s) 157, 594, 614, 673, 1129, 2100
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 7 cut(s) 95, 113, 552, 920, 1167, 1465, 1678
NsiI ATGCAT 3 cut(s) 1190, 1381, 2059
NspI RCATGY 4 cut(s) 917, 1401, 1636, 1915
PciI ACATGT 3 cut(s) 913, 1397, 1911
PctI GAATGC 1 cut(s) 1596
PfeI GAWTC 4 cut(s) 410, 1022, 1667, 2114
PkrI GCNGC 7 cut(s) 11, 67, 699, 1230, 1393, 2000, 2182
PleI GAGTC 1 cut(s) 1685
PpsI GAGTC 1 cut(s) 1685
PpuMI RGGWCCY 1 cut(s) 156
PscI ACATGT 3 cut(s) 913, 1397, 1911
PsiI TTATAA 2 cut(s) 1452, 1712
Psp1406I AACGTT 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 4 cut(s) 91, 109, 234, 508
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 4 cut(s) 91, 109, 234, 508
PspN4I GGNNCC 6 cut(s) 157, 594, 614, 673, 1129, 2100
PspPI GGNCC 5 cut(s) 156, 322, 593, 613, 1442
PspPPI RGGWCCY 1 cut(s) 156
PstI CTGCAG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 2098
PvuII CAGCTG 4 cut(s) 65, 700, 1228, 2183
RsaI GTAC 7 cut(s) 388, 429, 1545, 1631, 1754, 1925, 2355
RsaNI GTAC 7 cut(s) 387, 428, 1544, 1630, 1753, 1924, 2354
RseI CAYNNNNRTG 2 cut(s) 918, 2237
SatI GCNGC 7 cut(s) 10, 66, 698, 1229, 1392, 1999, 2181
Sau96I GGNCC 5 cut(s) 156, 322, 593, 613, 1442
ScaI AGTACT 2 cut(s) 388, 1754
SchI GAGTC 1 cut(s) 1686
ScrFI CCNGG 5 cut(s) 93, 111, 236, 510, 591
SduI GDGCHC 3 cut(s) 76, 546, 1246
SexAI ACCWGGT 2 cut(s) 91, 109
SfaNI GCATC 7 cut(s) 869, 1175, 1309, 1388, 1605, 1947, 2229
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 4 cut(s) 156, 322, 613, 1442
SmiMI CAYNNNNRTG 2 cut(s) 918, 2237
SmlI CTYRAG 2 cut(s) 1728, 1756
SmoI CTYRAG 2 cut(s) 1728, 1756
SpeI ACTAGT 3 cut(s) 383, 665, 1216
SsiI CCGC 8 cut(s) 330, 337, 648, 893, 1200, 1998, 2325, 2357
SspI AATATT 3 cut(s) 718, 1094, 1153
SspMI CTAG 7 cut(s) 6, 384, 666, 812, 1064, 1217, 2087
StyD4I CCNGG 5 cut(s) 91, 109, 234, 508, 589
StyI CCWWGG 2 cut(s) 448, 1048
TaaI ACNGT 4 cut(s) 391, 854, 1768, 2083
TaiI ACGT 2 cut(s) 311, 943
TaqI TCGA 5 cut(s) 294, 455, 930, 1309, 1665
TaqII GACCGA 1 cut(s) 339
TatI WGTACW 3 cut(s) 386, 1629, 1752
TauI GCSGC 1 cut(s) 2001
TfiI GAWTC 4 cut(s) 410, 1022, 1667, 2114
TscAI CASTG 3 cut(s) 232, 1138, 1527
TseFI GTSAC 7 cut(s) 95, 113, 552, 920, 1167, 1465, 1678
TseI GCWGC 6 cut(s) 9, 65, 697, 1228, 1391, 2180
Tsp45I GTSAC 7 cut(s) 95, 113, 552, 920, 1167, 1465, 1678
TspRI CASTG 3 cut(s) 232, 1138, 1527
VneI GTGCAC 2 cut(s) 72, 1242
VpaK11BI GGWCC 4 cut(s) 156, 322, 613, 1442
XapI RAATTY 5 cut(s) 193, 373, 827, 2090, 2316
XbaI TCTAGA 2 cut(s) 1063, 2086
XceI RCATGY 4 cut(s) 917, 1401, 1636, 1915
XspI CTAG 7 cut(s) 6, 384, 666, 812, 1064, 1217, 2087
ZrmI AGTACT 2 cut(s) 388, 1754
Zsp2I ATGCAT 3 cut(s) 1190, 1381, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.