RchiOBHm_Chr5g0025451

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000096
Physical Location & Seq
Reverse (-)
19343808 .. 19347140
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30510

Sequence Viewer

Length: 2577 bp
ATGGTTAATGTGATGATCATGTTTGCTGTTCAAGAACATCAGGAGAAAGCAAAGTATCTTCTGATCATCAACCATGGTTTTAGGTTTGATCAACTCTTAATTTTCTTCATGATCATTCTTTCAAGCTCGTGGAATTGTCATGTTGCTGCTGCAGCAGACAATATTGGGGACACACTCAAAATAGGCGACACTCTCAATTCCTCAAGCGCTTTAGTTTCCACCACTGGGAAGTTCACTTTGCTTTTCTTGCCCCTCAGTTCAAGTTCCAACTACAGCTATCTATATATTAGCCGGTACAATGTAAGTGGTGCGAATAGGGCTTGGGTTGGCAACCGAGACTCGCCTGTTCTATACCCTTTTGGAGTTCTTACCTTGGACTCCAACAACACATTAAAAATCATTACAACCAACGAAGGTGGCGGCGGCGATCCTCCTATAGTGCTTGGCTCCGTGCCAAAAAGTAGTAGTAATGTTGCGGCTACTCTGTTGGATTCTGGCAATTTTATCCTACAAGAGTTGAACTCCGATGGATCAACGAAGCAGGTGTGGTGGCAAAGTTTTGATTATCCAGCAGATACCTTTTTACCAGGCATGAAGTTAGGTTTCAACCGTAGCAACGGGCACATTTGGTCTCTTACGTCATGGACATCCGTTAGCTACCCAACGCCGGGGCCTTTCATCCTAGATTGGGACCCAACTGCCCGCCAATTGGAAATTCGGAGAGAAGGGGTGGTTTATTGGAGAAGCGGAAACTATAGTGCTACAAACAACAGATTCCAAAACATTTTGCCGAGCGCAACGGTTAGCTACACATTTAGCATTGTTTCAAACGAGAATGAAGACTACCTCACTTACACTTCTGAAGATGGTGCATATGAGTTACCAGAATGGATTCTAAATTATTATGGGAAGCTTTTTAATTATTATGGATCGAGAATTGATATTGCACGAGCAGATCAGTGTGGAGGCTACAACACAGATGGAGTAGGGTGCCAGACAACTGGCCGGCCAACTACTTGTATGCCGGATTTCGTTAGTCCATTTGGGCTAAAAAAAGGTTCCTTTAAACCAATCACCTCGAGTTCAACTTCAAGATGCCCATACTCGCTCAACTTTGTATCAAATGGAAGTCACAGTGATACTAGTATTGACTGTAAGAATACTTGTTTGCAAAACTGTGACTGCCTTGGATTCGACTTTCTATTTGACAATCAGACTGGATGCCGATTTTGGAGTGTGGACTGTGAATTCCTTGAAGACCGCACTCAACCTGGTAATGCAAGCAGTTTGGTTTTAACAAAGCTAATGCCAACAGCAAAATCACCAGCGCCGAAGTCACCACCAAGCAAAACTGGTGCAACGCATAAATGGATTTGGATTGGGGTTGCTATTAGTGCTGCTTTAGTGATGATGGCCTTTTCCATCTTGTGCTGTCTACGAAGAAGAAAAAGACAATCTAAACATTCAGGTGAGGGTAAGATCGACCAGAATGAATTGCTGAATTTCAGGAATTCTAAGAGGCCTACTGATTTGAATGGACTTCAAAATGATGGAAAGATGGGACATGATTTGAGCGTATTTAGCTATGCATCTGTAATGGCTGCCACAATCAACTTCGCTGAAGAAAACAAGCTTGGAGAAGGGGGCTTTGGACCTGTTTATAAGGGGAAATTGGCAACGGGACAAGAAGTAGCTGTGAAGAGGCTTTCAAAATGTTCAGGGCAAGGAACACTAGAGTTTAAGAATGAATTGATCCTCATACATGAACTTCAACATACAAACCTTGTCCAGCTTTTTGGATTTTGCATTCACGGTGAAGAGAGGATGTTGATATATGAGTACATGCCAAACAAAAGTCTGGACTACTTTTTATTTAATTCAATCAGAAGTGTTTTTCTCGATTGGAGCAAGCGTTTCAGCATAATTGAAGGAATCGCTCAAGGATTGCTTTATTTGCACAAATTCTCAAGAACAAGAGTAATTCATAGAGATTTAAAAGCCAGTAACGTACTACTTGATGAAAATATGAACCCCAAAATTTCAGATTTTGGTATGGCTAGGATTTTCTCCATTAATGAACTGGAAGCAAATACTAGTAGGATTGTTGGGACACGTGGTTACATGCCTCCTGAGTACGCAATGGAAGGAATTTTCTCTACAAAATCCGATGTCTACAGTTTTGGAGTGTTAGTGCTTGAAATCATAAGTGGTAGGAGAAACAATAGCTTCTACAATGATGATCGCGCCATCAATTTAGTAGGATATGCATGGGAGTTGTGGAAAGAAGGCGCAGGGCTACAACTAATGGATCCAACACTAGCTGATTCGTGTACTAACAAAGATCAACTGTTAATATGCGTTCAAGTTGGTCTTCTATGCGTGGAGGATAGTGCAGCGAATCGACCTACCATGTCAGATGTGCTCTCCGCGTTGACAAATGAAAGCTCACCATTACCTGCACCTACAAAGCCAGCATTTTCTACAGATAGACATGCGATCACCAGTAGTATAGTTGAAAAGGAACCACAATCTTTATCTGTAAATGATCTGACCATTTCCGCTTGTTATGGACGTTAA

Protein Analysis

858

Amino Acids

95.27

Weight (kDa)

6.21

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 108 - 215 1.9e-23 D-mannose binding lectin
Pkinase PF00069 540 - 740 5.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 542 - 742 4.9e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1662
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 2 cut(s) 532, 2464
AccB1I GGYRCC 1 cut(s) 990
AccI GTMKAC 2 cut(s) 1435, 2172
AccII CGCG 2 cut(s) 2244, 2429
AciI CCGC 8 cut(s) 420, 423, 476, 703, 747, 1261, 2427, 2559
AclWI GGATC 6 cut(s) 422, 538, 937, 1747, 2303, 2316
AcoI YGGCCR 2 cut(s) 1003, 1007
AcsI RAATTY 7 cut(s) 714, 1247, 1501, 1510, 1961, 2037, 2148
AcuI CTGAAG 2 cut(s) 882, 1641
AcvI CACGTG 1 cut(s) 2114
AfaI GTAC 5 cut(s) 296, 1841, 2010, 2135, 2332
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 5 cut(s) 225, 667, 668, 688, 709
AflIII ACRYGT 1 cut(s) 2111
AhlI ACTAGT 2 cut(s) 1142, 2093
AjnI CCWGG 2 cut(s) 586, 1270
AjuI GAANNNNNNNTTGG 6 cut(s) 699, 731, 1617, 1632, 1649, 1664
Alw21I GWGCWC 1 cut(s) 2424
Alw26I GTCTC 2 cut(s) 330, 636
AlwI GGATC 6 cut(s) 422, 538, 937, 1747, 2303, 2316
Ama87I CYCGRG 1 cut(s) 1078
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 5 cut(s) 671, 1003, 1007, 1413, 1520
ApeKI GCWGC 6 cut(s) 146, 149, 152, 1397, 1601, 2393
ApoI RAATTY 7 cut(s) 714, 1247, 1501, 1510, 1961, 2037, 2148
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AseI ATTAAT 1 cut(s) 2073
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 5 cut(s) 209, 797, 1330, 2246, 2291
AspS9I GGNCC 3 cut(s) 671, 691, 1652
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 7 cut(s) 1066, 1314, 1329, 1481, 1826, 2439, 2491
AvaI CYCGRG 1 cut(s) 1078
AvaII GGWCC 2 cut(s) 691, 1652
BaeGI GKGCMC 1 cut(s) 624
BamHI GGATCC 1 cut(s) 2308
BanI GGYRCC 1 cut(s) 990
BauI CACGAG 2 cut(s) 127, 948
BbrPI CACGTG 1 cut(s) 2114
BbsI GAAGAC 3 cut(s) 846, 1263, 2363
Bbv12I GWGCWC 1 cut(s) 2424
BbvI GCAGC 6 cut(s) 133, 136, 164, 1384, 1588, 2405
BccI CCATC 8 cut(s) 521, 860, 974, 1405, 1430, 1544, 1552, 2255
BciT130I CCWGG 2 cut(s) 588, 1272
BclI TGATCA 4 cut(s) 15, 63, 88, 111
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 330, 636
BcuI ACTAGT 2 cut(s) 1142, 2093
BfaI CTAG 6 cut(s) 683, 1143, 1733, 2058, 2094, 2318
BfmI CTRYAG 6 cut(s) 150, 271, 435, 754, 2173, 2481
BfoI RGCGCY 2 cut(s) 210, 1331
BfuAI ACCTGC 2 cut(s) 532, 2464
BisI GCNGC 9 cut(s) 147, 150, 153, 421, 424, 477, 1398, 1602, 2394
BlsI GCNGC 9 cut(s) 148, 151, 154, 422, 425, 478, 1399, 1603, 2395
Bme1390I CCNGG 3 cut(s) 588, 669, 1272
Bme18I GGWCC 2 cut(s) 691, 1652
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 3 cut(s) 671, 691, 1652
BmiI GGNNCC 8 cut(s) 448, 672, 692, 693, 992, 1060, 2310, 2523
BmrFI CCNGG 3 cut(s) 588, 669, 1272
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 3 cut(s) 1085, 1211, 1598
BmuI ACTGGG 1 cut(s) 234
BpiI GAAGAC 3 cut(s) 846, 1263, 2363
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BpuEI CTTGAG 3 cut(s) 187, 1923, 1951
BpuMI CCSGG 1 cut(s) 669
BsaAI YACGTR 1 cut(s) 2114
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 73, 372, 668, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 1897, 1927, 2175, 2205
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 667, 668, 688, 709
Bse118I RCCGGY 2 cut(s) 291, 1005
Bse1I ACTGG 7 cut(s) 229, 1006, 1222, 1357, 2001, 2085, 2502
Bse3DI GCAATG 1 cut(s) 2145
BseBI CCWGG 2 cut(s) 588, 1272
BseDI CCNNGG 4 cut(s) 73, 372, 668, 1186
BseGI GGATG 4 cut(s) 647, 678, 1226, 1830
BseLI CCNNNNNNNGG 5 cut(s) 225, 667, 668, 688, 709
BseMI GCAATG 1 cut(s) 2145
BseMII CTCAG 2 cut(s) 268, 2121
BseNI ACTGG 7 cut(s) 229, 1006, 1222, 1357, 2001, 2085, 2502
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 6 cut(s) 133, 136, 164, 1384, 1588, 2405
BsgI GTGCAG 2 cut(s) 2412, 2442
Bsh1236I CGCG 2 cut(s) 2244, 2429
BshFI GGCC 5 cut(s) 673, 1005, 1009, 1415, 1522
BshNI GGYRCC 1 cut(s) 990
BsiHKAI GWGCWC 1 cut(s) 2424
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 4 cut(s) 292, 668, 1006, 1025
BslFI GGGAC 5 cut(s) 182, 704, 1575, 1695, 2122
BslI CCNNNNNNNGG 5 cut(s) 225, 667, 668, 688, 709
BsmAI GTCTC 2 cut(s) 330, 636
BsmFI GGGAC 5 cut(s) 182, 704, 1575, 1695, 2122
BsmI GAATGC 1 cut(s) 1806
BsnI GGCC 5 cut(s) 673, 1005, 1009, 1415, 1522
Bso31I GGTCTC 1 cut(s) 636
BsoBI CYCGRG 1 cut(s) 1078
Bsp1286I GDGCHC 2 cut(s) 624, 2424
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 8 cut(s) 420, 423, 476, 703, 747, 1261, 2427, 2559
BspANI GGCC 5 cut(s) 673, 1005, 1009, 1415, 1522
BspCNI CTCAG 2 cut(s) 267, 2122
BspFNI CGCG 2 cut(s) 2244, 2429
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 8 cut(s) 448, 672, 692, 693, 992, 1060, 2310, 2523
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 2 cut(s) 532, 2464
BspPI GGATC 6 cut(s) 422, 538, 937, 1747, 2303, 2316
BspT107I GGYRCC 1 cut(s) 990
BspTNI GGTCTC 1 cut(s) 636
BsrDI GCAATG 1 cut(s) 2145
BsrFI RCCGGY 2 cut(s) 291, 1005
BsrI ACTGG 7 cut(s) 229, 1006, 1222, 1357, 2001, 2085, 2502
BssAI RCCGGY 2 cut(s) 291, 1005
BssECI CCNNGG 4 cut(s) 73, 372, 668, 1186
BssSI CACGAG 2 cut(s) 127, 948
BssT1I CCWWGG 3 cut(s) 73, 372, 1186
Bst2BI CACGAG 2 cut(s) 127, 948
Bst2UI CCWGG 2 cut(s) 588, 1272
Bst4CI ACNGT 9 cut(s) 611, 802, 1136, 1154, 1178, 1244, 1814, 2177, 2349
Bst6I CTCTTC 2 cut(s) 1694, 1812
BstBAI YACGTR 1 cut(s) 2114
BstC8I GCNNGC 5 cut(s) 703, 1007, 1282, 1910, 2472
BstDEI CTNAG 3 cut(s) 254, 1515, 2130
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 4 cut(s) 647, 678, 1226, 1830
BstFNI CGCG 2 cut(s) 2244, 2429
BstH2I RGCGCY 2 cut(s) 210, 1331
BstHHI GCGC 5 cut(s) 209, 797, 1330, 2246, 2291
BstMAI GTCTC 2 cut(s) 330, 636
BstMWI GCNNNNNNNGC 6 cut(s) 152, 247, 317, 1394, 1581, 1954
BstNI CCWGG 2 cut(s) 588, 1272
BstNSI RCATGY 3 cut(s) 1846, 2125, 2495
BstSCI CCNGG 3 cut(s) 586, 667, 1270
BstSFI CTRYAG 6 cut(s) 150, 271, 435, 754, 2173, 2481
BstSLI GKGCMC 1 cut(s) 624
BstUI CGCG 2 cut(s) 2244, 2429
BstV1I GCAGC 6 cut(s) 133, 136, 164, 1384, 1588, 2405
BstV2I GAAGAC 3 cut(s) 846, 1263, 2363
BstX2I RGATCY 1 cut(s) 2308
BstXI CCANNNNNNTGG 2 cut(s) 1001, 1796
BstYI RGATCY 1 cut(s) 2308
BsuRI GGCC 5 cut(s) 673, 1005, 1009, 1415, 1522
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 4 cut(s) 647, 678, 1226, 1830
BtsIMutI CAGTG 3 cut(s) 222, 965, 1141
BveI ACCTGC 2 cut(s) 532, 2464
Cac8I GCNNGC 5 cut(s) 703, 1007, 1282, 1910, 2472
CciI TCATGA 1 cut(s) 108
CfoI GCGC 5 cut(s) 209, 797, 1330, 2246, 2291
Cfr10I RCCGGY 2 cut(s) 291, 1005
Cfr13I GGNCC 3 cut(s) 671, 691, 1652
CsiI ACCWGGT 1 cut(s) 1270
Csp6I GTAC 5 cut(s) 295, 1840, 2009, 2134, 2331
CspCI CAANNNNNGTGG 4 cut(s) 286, 321, 397, 432
CviQI GTAC 5 cut(s) 295, 1840, 2009, 2134, 2331
DdeI CTNAG 3 cut(s) 254, 1515, 2130
DraI TTTAAA 2 cut(s) 1066, 1995
EaeI YGGCCR 2 cut(s) 1003, 1007
Eam1104I CTCTTC 2 cut(s) 1694, 1812
EarI CTCTTC 2 cut(s) 1694, 1812
Eco130I CCWWGG 3 cut(s) 73, 372, 1186
Eco147I AGGCCT 1 cut(s) 1522
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 691, 1652
Eco47III AGCGCT 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 882, 1641
Eco72I CACGTG 1 cut(s) 2114
Eco88I CYCGRG 1 cut(s) 1078
EcoO109I RGGNCCY 2 cut(s) 671, 691
EcoRI GAATTC 2 cut(s) 1247, 1510
EcoRII CCWGG 2 cut(s) 586, 1270
EcoT14I CCWWGG 3 cut(s) 73, 372, 1186
EcoT22I ATGCAT 2 cut(s) 1591, 2269
ErhI CCWWGG 3 cut(s) 73, 372, 1186
FalI AAGNNNNNCTT 4 cut(s) 1932, 1964, 2355, 2387
FaqI GGGAC 5 cut(s) 182, 704, 1575, 1695, 2122
FauI CCCGC 1 cut(s) 710
FauNDI CATATG 1 cut(s) 874
FbaI TGATCA 4 cut(s) 15, 63, 88, 111
FblI GTMKAC 2 cut(s) 1435, 2172
Fnu4HI GCNGC 9 cut(s) 147, 150, 153, 421, 424, 477, 1398, 1602, 2394
FokI GGATG 4 cut(s) 634, 665, 1233, 1837
FseI GGCCGGCC 1 cut(s) 1009
Fsp4HI GCNGC 9 cut(s) 147, 150, 153, 421, 424, 477, 1398, 1602, 2394
FspBI CTAG 6 cut(s) 683, 1143, 1733, 2058, 2094, 2318
GlaI GCGC 5 cut(s) 208, 796, 1329, 2245, 2290
GluI GCNGC 9 cut(s) 147, 150, 153, 421, 424, 477, 1398, 1602, 2394
HaeII RGCGCY 2 cut(s) 210, 1331
HaeIII GGCC 5 cut(s) 673, 1005, 1009, 1415, 1522
HapII CCGG 4 cut(s) 292, 668, 1006, 1025
HhaI GCGC 5 cut(s) 209, 797, 1330, 2246, 2291
Hin6I GCGC 5 cut(s) 207, 795, 1328, 2244, 2289
HinP1I GCGC 5 cut(s) 207, 795, 1328, 2244, 2289
HincII GTYRAC 1 cut(s) 2433
HindII GTYRAC 1 cut(s) 2433
HindIII AAGCTT 2 cut(s) 911, 1631
HinfI GANTC 9 cut(s) 338, 377, 491, 774, 892, 1191, 1932, 2324, 2398
HpaII CCGG 4 cut(s) 292, 668, 1006, 1025
HphI GGTGA 7 cut(s) 1066, 1314, 1329, 1481, 1826, 2439, 2491
Hpy166II GTNNAC 6 cut(s) 234, 1240, 1436, 2173, 2331, 2433
Hpy8I GTNNAC 6 cut(s) 234, 1240, 1436, 2173, 2331, 2433
HpyAV CCTTC 6 cut(s) 407, 719, 1634, 1922, 2138, 2279
HpyCH4III ACNGT 9 cut(s) 611, 802, 1136, 1154, 1178, 1244, 1814, 2177, 2349
HpyCH4IV ACGT 4 cut(s) 638, 2007, 2113, 2572
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 247, 317, 1394, 1581, 1954
HpyF3I CTNAG 3 cut(s) 254, 1515, 2130
HpySE526I ACGT 4 cut(s) 638, 2007, 2113, 2572
HspAI GCGC 5 cut(s) 207, 795, 1328, 2244, 2289
KflI GGGWCCC 1 cut(s) 691
KroI GCCGGC 1 cut(s) 1005
KroNI GCCGGC 1 cut(s) 1007
Ksp22I TGATCA 4 cut(s) 15, 63, 88, 111
LmnI GCTCC 2 cut(s) 452, 1905
Lsp1109I GCAGC 6 cut(s) 133, 136, 164, 1384, 1588, 2405
LweI GCATC 3 cut(s) 1085, 1211, 1598
MabI ACCWGGT 1 cut(s) 1270
MaeI CTAG 6 cut(s) 683, 1143, 1733, 2058, 2094, 2318
MaeII ACGT 4 cut(s) 638, 2007, 2113, 2572
MaeIII GTNAC 6 cut(s) 879, 1130, 1178, 1335, 2003, 2117
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 1 cut(s) 2308
MhlI GDGCHC 2 cut(s) 624, 2424
MlyI GAGTC 2 cut(s) 332, 371
MmeI TCCRAC 4 cut(s) 291, 405, 468, 2336
Mph1103I ATGCAT 2 cut(s) 1591, 2269
MroNI GCCGGC 1 cut(s) 1005
MroXI GAANNNNTTC 1 cut(s) 891
MslI CAYNNNNRTG 1 cut(s) 1467
MspI CCGG 4 cut(s) 292, 668, 1006, 1025
MspR9I CCNGG 3 cut(s) 588, 669, 1272
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 1806
MvaI CCWGG 2 cut(s) 588, 1272
MvnI CGCG 2 cut(s) 2244, 2429
MwoI GCNNNNNNNGC 6 cut(s) 152, 247, 317, 1394, 1581, 1954
NaeI GCCGGC 1 cut(s) 1007
NciI CCSGG 1 cut(s) 669
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 874
NgoMIV GCCGGC 1 cut(s) 1005
NlaIV GGNNCC 8 cut(s) 448, 672, 692, 693, 992, 1060, 2310, 2523
NmeAIII GCCGAG 1 cut(s) 816
NmuCI GTSAC 3 cut(s) 1130, 1178, 1335
NsiI ATGCAT 2 cut(s) 1591, 2269
NspI RCATGY 3 cut(s) 1846, 2125, 2495
PaeR7I CTCGAG 1 cut(s) 1078
PagI TCATGA 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 532
PceI AGGCCT 1 cut(s) 1522
PctI GAATGC 1 cut(s) 1806
PdiI GCCGGC 1 cut(s) 1007
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 7 cut(s) 491, 774, 892, 1191, 1932, 2324, 2398
PkrI GCNGC 9 cut(s) 148, 151, 154, 422, 425, 478, 1399, 1603, 2395
PleI GAGTC 2 cut(s) 332, 371
PmaCI CACGTG 1 cut(s) 2114
PmlI CACGTG 1 cut(s) 2114
PpsI GAGTC 2 cut(s) 332, 371
Ppu21I YACGTR 1 cut(s) 2114
PpuMI RGGWCCY 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 2073
PsiI TTATAA 1 cut(s) 1662
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 2 cut(s) 586, 1270
PspCI CACGTG 1 cut(s) 2114
PspGI CCWGG 2 cut(s) 586, 1270
PspN4I GGNNCC 8 cut(s) 448, 672, 692, 693, 992, 1060, 2310, 2523
PspPI GGNCC 3 cut(s) 671, 691, 1652
PspPPI RGGWCCY 1 cut(s) 691
PspXI VCTCGAGB 1 cut(s) 1078
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 2308
RigI GGCCGGCC 1 cut(s) 1009
RsaI GTAC 5 cut(s) 296, 1841, 2010, 2135, 2332
RsaNI GTAC 5 cut(s) 295, 1840, 2009, 2134, 2331
RseI CAYNNNNRTG 1 cut(s) 1467
SatI GCNGC 9 cut(s) 147, 150, 153, 421, 424, 477, 1398, 1602, 2394
Sau96I GGNCC 3 cut(s) 671, 691, 1652
SchI GAGTC 2 cut(s) 332, 371
ScrFI CCNGG 3 cut(s) 588, 669, 1272
SduI GDGCHC 2 cut(s) 624, 2424
SexAI ACCWGGT 1 cut(s) 1270
SfaNI GCATC 3 cut(s) 1085, 1211, 1598
SfcI CTRYAG 6 cut(s) 150, 271, 435, 754, 2173, 2481
Sfr274I CTCGAG 1 cut(s) 1078
SinI GGWCC 2 cut(s) 691, 1652
SlaI CTCGAG 1 cut(s) 1078
SmiMI CAYNNNNRTG 1 cut(s) 1467
SmlI CTYRAG 4 cut(s) 202, 1078, 1938, 1966
SmoI CTYRAG 4 cut(s) 202, 1078, 1938, 1966
SpeI ACTAGT 2 cut(s) 1142, 2093
SseBI AGGCCT 1 cut(s) 1522
SsiI CCGC 8 cut(s) 420, 423, 476, 703, 747, 1261, 2427, 2559
SspI AATATT 1 cut(s) 163
SspMI CTAG 6 cut(s) 683, 1143, 1733, 2058, 2094, 2318
StuI AGGCCT 1 cut(s) 1522
StyD4I CCNGG 3 cut(s) 586, 667, 1270
StyI CCWWGG 3 cut(s) 73, 372, 1186
TaaI ACNGT 9 cut(s) 611, 802, 1136, 1154, 1178, 1244, 1814, 2177, 2349
TaiI ACGT 4 cut(s) 641, 2010, 2116, 2575
TaqI TCGA 6 cut(s) 932, 1079, 1194, 1482, 1899, 2401
TatI WGTACW 2 cut(s) 1839, 2330
TauI GCSGC 3 cut(s) 423, 426, 479
TfiI GAWTC 7 cut(s) 491, 774, 892, 1191, 1932, 2324, 2398
TscAI CASTG 3 cut(s) 229, 965, 1141
TseFI GTSAC 3 cut(s) 1130, 1178, 1335
TseI GCWGC 6 cut(s) 146, 149, 152, 1397, 1601, 2393
Tsp45I GTSAC 3 cut(s) 1130, 1178, 1335
TspGWI ACGGA 2 cut(s) 439, 640
TspRI CASTG 3 cut(s) 229, 965, 1141
VpaK11BI GGWCC 2 cut(s) 691, 1652
VspI ATTAAT 1 cut(s) 2073
XapI RAATTY 7 cut(s) 714, 1247, 1501, 1510, 1961, 2037, 2148
XceI RCATGY 3 cut(s) 1846, 2125, 2495
XcmI CCANNNNNNNNNTGG 1 cut(s) 2077
XhoI CTCGAG 1 cut(s) 1078
XmiI GTMKAC 2 cut(s) 1435, 2172
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 6 cut(s) 683, 1143, 1733, 2058, 2094, 2318
Zsp2I ATGCAT 2 cut(s) 1591, 2269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.