Rroxscaffold_1G00054130

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000096
Physical Location & Seq
Forward (+)
75439807 .. 75443571
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054130.1

Sequence Viewer

Length: 2418 bp
ATGGGAAGGCCAGCAGCGCAAGGCCTGCACCGAAGATGCTGCTCCGATATGGTCATCGGGACGCTGCCCGGCATGGTCATCGGGACGCTGCCCGGCATGGTCATCGGGACGCTGCCCGGCATGGTCATCGGGACGCTGCCCGGCATGTTCATCGGAGACGCTGCCCGGCATGTTCATCGGAGACGCTGCTCCGGCATGTTGGTTTTGACATCATTGACACCCGAAGGTGCCGTTGTGAGGAGGGCCTGCAACAAGAAAGAGAAGGTGGTGGAGTGCTATGGAATGAGCATTCCAATGATCAACTTATTGTGCCTCATCAGTTTCTCAAGCTTCTGGACTTGTCATGGTGCAGCTGCACTGTTCATTGGAGACATGCTTAATCCAGGGGAGGCCCTCAATTCCTCGAGCTTTATGGTCTCCGCTTCGAGGGAGTTTACGTTGGGTTTCAACTCCGGCTACCTCATTTTGAGGTGCAAGAACTATACGGTTTGGGTTGCCAACCTGCACACTCCGATTTCAGACCCTTCGTCGGCGCTTCTCACCTTGGACAAGAACAGAACTGTCCTGCAAATTACCCTGAAAGGCAAGAATCCCATCGTTCTTTACTCCTCTTTTGATGATAATAGTACCAATGTTGTGGCTACCCTGTCAGATTCTGGCAATTTTGTCCTACGAGAGGTGAATCAGAACGTTTCAAACTCTCCGTTGTGGCAAAGCTTTGATGGTCCTAATGTGGCTCTTAGGCCGGGGATGAAATTAGGTTTTGATGCTGGTCTAACTATAACTAGCAACAGTATGTCGATTTGGAGTTGGCAGGAAAATCAGAACAGCCCAGGGCCAGGGTCTTTCAGCCTTTATTGGGATTCCGGCACACGCCAATTGCAAGTTAGGAGAAACGGGGATCTTTGGTGGACTAGCGAAACATTGAGAAGTGGAAGATACGAGTATGCTGATTTCCGTAATAAATCCAAGGTCTACAATTTTAGCGTGGTTTCATATGAGAATGGAGACTACTTCAGTTACACTGTCGTTGTAGGTGACTGTGATGGCTATAATTATAACATCAAATTCACCGATCGCTATAACTCCGTTGGTGGGACGTGCCAGAGATGGAGTGGCCGGGCCACTCTTTATTGTCCTGTGCCAACATCCACTGGTGCATTTATTCAGCAGACAGAAAGGCGCTCCTTTAGACCAATCAGTATGAACATGTCTATCAATTCAACATGGGAGGAGACAAATTCAAGTCGATATGGGATTTATGAATATGCATCACATAAGTGGATATGGATTGGTATTGCAATTGCAGCTGTTCTTCTGGTGCTGGTGCTTTGCAGCATCTGCTACCTAATACGAAAAAGAAAACTTGCAGGTGAGAACCAAACAAATATCCAGGATATGCTGAACATCATGAAATCTAATAGACCTACTGATGCTAATGGGCTTCAAAATGATGGAAACAGGGGACAAGATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACTTGCAACTTCTCTAATGAAAATAAGCCCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGGAAATTGGTGACTGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAACGTTCAGGGCAAGGAATATATGAGTTTAAGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGTTGTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAAAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAGTTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAATATTCCAGAATGAGAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACGAATGATGAACAAGAAGCAAATACTGTGAAGATTGTTGGGACACGTTTCGGTGTGTTGATGCTTGAAATCCTAAGCGGTAGAAGAAACAACAGCTTCTACAATGATGATCGCGCCCTCAATTTAGTTGGTTATGCATGGGCGCTATGGAAAGAAGGTGCAGGACTAGAACTAATGGATCCAACACTTGGTGATTCATGTGATAAAGATCATTTGTTGAGGTGCATCCATGTTGGTTTGCTCTGCGTAGAGGAAAATGCAGCCAGTCGACCAAAGATGTTAGATGTGATATCTATGCTGACTAATGAAAGCATGTCACTGCCAGTACCTATAAAACCAGCATTTTGTATAGAAAGAAATGTCATCACAACTACTATAGGTGGAAACAGACCGGAAGTGGTGGTATCGGTAAATGGTATCTCCAATTCTGATTTTGATGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

805

Amino Acids

89.85

Weight (kDa)

8.36

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 162 - 271 2.6e-17 D-mannose binding lectin
Pkinase PF00069 509 - 673 9.3e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 511 - 665 9.1e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1059, 1566
AarI CACCTGC 1 cut(s) 1361
Acc36I ACCTGC 2 cut(s) 510, 1361
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 2159
AccI GTMKAC 2 cut(s) 975, 2239
AccII CGCG 1 cut(s) 2085
AciI CCGC 2 cut(s) 420, 2049
AclI AACGTT 2 cut(s) 690, 1618
AclWI GGATC 3 cut(s) 909, 2144, 2157
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 3 cut(s) 1067, 1240, 1941
AcuI CTGAAG 1 cut(s) 1000
AdeI CACNNNGTG 1 cut(s) 2162
AfaI GTAC 3 cut(s) 628, 1745, 2298
AflIII ACRYGT 2 cut(s) 1209, 2015
AhdI GACNNNNNGTC 1 cut(s) 526
AjiI CACGTC 1 cut(s) 1101
AjnI CCWGG 4 cut(s) 382, 832, 838, 1392
AjuI GAANNNNNNNTTGG 2 cut(s) 1536, 1568
AleI CACNNNNGTG 1 cut(s) 1279
AluBI AGCT 8 cut(s) 330, 353, 408, 717, 1310, 1505, 1761, 2067
AluI AGCT 8 cut(s) 330, 353, 408, 717, 1310, 1505, 1761, 2067
Alw26I GTCTC 6 cut(s) 150, 175, 363, 421, 1002, 1229
AlwI GGATC 3 cut(s) 909, 2144, 2157
AlwNI CAGNNNCTG 2 cut(s) 656, 1341
Ama87I CYCGRG 1 cut(s) 403
AoxI GGCC 8 cut(s) 8, 22, 243, 390, 743, 836, 1117, 1122
ApoI RAATTY 3 cut(s) 1067, 1240, 1941
AspLEI GCGC 5 cut(s) 19, 535, 1185, 2087, 2116
AspS9I GGNCC 6 cut(s) 243, 391, 725, 836, 1122, 1556
AsuC2I CCSGG 8 cut(s) 69, 93, 117, 141, 166, 747, 1121, 1539
AsuHPI GGTGA 8 cut(s) 532, 691, 1049, 1063, 1385, 1591, 1730, 2174
AvaI CYCGRG 1 cut(s) 403
AvaII GGWCC 2 cut(s) 725, 1556
BamHI GGATCC 1 cut(s) 2149
BanI GGYRCC 1 cut(s) 227
BccI CCATC 6 cut(s) 602, 716, 1040, 1104, 1448, 2402
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 4 cut(s) 384, 834, 840, 1394
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 8 cut(s) 69, 93, 117, 141, 166, 747, 1121, 1539
BcoDI GTCTC 6 cut(s) 150, 175, 363, 421, 1002, 1229
BfaI CTAG 4 cut(s) 786, 915, 1788, 2138
BfmI CTRYAG 1 cut(s) 2346
BfoI RGCGCY 3 cut(s) 536, 1186, 2117
BfuAI ACCTGC 2 cut(s) 510, 1361
Bme18I GGWCC 2 cut(s) 725, 1556
BmeRI GACNNNNNGTC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 403
BmgBI CACGTC 1 cut(s) 1101
BmgT120I GGNCC 6 cut(s) 243, 391, 725, 836, 1122, 1556
BmiI GGNNCC 2 cut(s) 229, 2151
BmsI GCATC 9 cut(s) 26, 757, 1280, 1347, 1423, 1502, 1781, 2022, 2205
BpmI CTGGAG 1 cut(s) 1658
Bpu10I CCTNAGC 1 cut(s) 2045
BpuEI CTTGAG 2 cut(s) 310, 1827
BpuMI CCSGG 8 cut(s) 69, 93, 117, 141, 166, 747, 1121, 1539
BsaBI GATNNNNATC 1 cut(s) 2178
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 7 cut(s) 383, 543, 746, 832, 833, 839, 969
BsaWI WCCGGW 1 cut(s) 2362
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bse1I ACTGG 5 cut(s) 1159, 1588, 1811, 2235, 2294
Bse8I GATNNNNATC 1 cut(s) 2178
BseBI CCWGG 4 cut(s) 384, 834, 840, 1394
BseDI CCNNGG 7 cut(s) 383, 543, 746, 832, 833, 839, 969
BseGI GGATG 4 cut(s) 756, 1148, 1734, 2196
BseJI GATNNNNATC 1 cut(s) 2178
BseNI ACTGG 5 cut(s) 1159, 1588, 1811, 2235, 2294
BseRI GAGGAG 3 cut(s) 253, 598, 1247
BsgI GTGCAG 5 cut(s) 11, 339, 369, 488, 2151
Bsh1236I CGCG 1 cut(s) 2085
Bsh1285I CGRYCG 1 cut(s) 1078
BshFI GGCC 8 cut(s) 10, 24, 245, 392, 745, 838, 1119, 1124
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 1078
BsiHKCI CYCGRG 1 cut(s) 403
BslFI GGGAC 7 cut(s) 73, 97, 121, 145, 1111, 1479, 2026
BsmAI GTCTC 6 cut(s) 150, 175, 363, 421, 1002, 1229
BsmBI CGTCTC 2 cut(s) 150, 175
BsmFI GGGAC 7 cut(s) 73, 97, 121, 145, 1111, 1479, 2026
BsmI GAATGC 2 cut(s) 288, 1710
BsnI GGCC 8 cut(s) 10, 24, 245, 392, 745, 838, 1119, 1124
Bso31I GGTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 403
Bsp143I GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
BspACI CCGC 2 cut(s) 420, 2049
BspANI GGCC 8 cut(s) 10, 24, 245, 392, 745, 838, 1119, 1124
BspFNI CGCG 1 cut(s) 2085
BspHI TCATGA 1 cut(s) 1410
BspLI GGNNCC 2 cut(s) 229, 2151
BspMI ACCTGC 2 cut(s) 510, 1361
BspPI GGATC 3 cut(s) 909, 2144, 2157
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 421
BsrI ACTGG 5 cut(s) 1159, 1588, 1811, 2235, 2294
BssECI CCNNGG 7 cut(s) 383, 543, 746, 832, 833, 839, 969
BssMI GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
BssT1I CCWWGG 2 cut(s) 543, 969
Bst2UI CCWGG 4 cut(s) 384, 834, 840, 1394
Bst4CI ACNGT 8 cut(s) 360, 487, 562, 794, 1027, 1043, 1817, 1999
Bst6I CTCTTC 1 cut(s) 1716
BstAPI GCANNNNNTGC 2 cut(s) 25, 1341
BstC8I GCNNGC 4 cut(s) 12, 26, 247, 1796
BstDEI CTNAG 2 cut(s) 740, 2045
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 4 cut(s) 756, 1148, 1734, 2196
BstFNI CGCG 1 cut(s) 2085
BstH2I RGCGCY 3 cut(s) 536, 1186, 2117
BstHHI GCGC 5 cut(s) 19, 535, 1185, 2087, 2116
BstKTI GATC 6 cut(s) 300, 904, 1078, 2083, 2152, 2182
BstMAI GTCTC 6 cut(s) 150, 175, 363, 421, 1002, 1229
BstMBI GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
BstMCI CGRYCG 1 cut(s) 1078
BstMWI GCNNNNNNNGC 5 cut(s) 16, 25, 192, 1307, 1341
BstNI CCWGG 4 cut(s) 384, 834, 840, 1394
BstNSI RCATGY 7 cut(s) 148, 173, 199, 376, 1213, 1750, 2287
BstSFI CTRYAG 1 cut(s) 2346
BstUI CGCG 1 cut(s) 2085
BstX2I RGATCY 2 cut(s) 901, 2149
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 2 cut(s) 901, 2149
BsuRI GGCC 8 cut(s) 10, 24, 245, 392, 745, 838, 1119, 1124
BtrI CACGTC 1 cut(s) 1101
BtsCI GGATG 4 cut(s) 756, 1148, 1734, 2196
BtsI GCAGTG 2 cut(s) 1605, 2288
BtsIMutI CAGTG 5 cut(s) 356, 1023, 1152, 1605, 2288
BveI ACCTGC 2 cut(s) 510, 1361
Cac8I GCNNGC 4 cut(s) 12, 26, 247, 1796
CaiI CAGNNNCTG 2 cut(s) 656, 1341
CciI TCATGA 1 cut(s) 1410
CfoI GCGC 5 cut(s) 19, 535, 1185, 2087, 2116
Cfr13I GGNCC 6 cut(s) 243, 391, 725, 836, 1122, 1556
CseI GACGC 6 cut(s) 70, 94, 118, 142, 167, 192
Csp6I GTAC 3 cut(s) 627, 1744, 2297
CviQI GTAC 3 cut(s) 627, 1744, 2297
DdeI CTNAG 2 cut(s) 740, 2045
DpnI GATC 6 cut(s) 299, 903, 1077, 2082, 2151, 2181
DpnII GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
DraIII CACNNNGTG 1 cut(s) 2162
DriI GACNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 1 cut(s) 1716
Eam1105I GACNNNNNGTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 1716
Eco130I CCWWGG 2 cut(s) 543, 969
Eco147I AGGCCT 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 421
Eco32I GATATC 1 cut(s) 2262
Eco47I GGWCC 2 cut(s) 725, 1556
Eco57I CTGAAG 1 cut(s) 1000
Eco88I CYCGRG 1 cut(s) 403
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO109I RGGNCCY 2 cut(s) 243, 391
EcoRII CCWGG 4 cut(s) 382, 832, 838, 1392
EcoRV GATATC 1 cut(s) 2262
EcoT14I CCWWGG 2 cut(s) 543, 969
EcoT22I ATGCAT 3 cut(s) 1273, 1495, 2110
ErhI CCWWGG 2 cut(s) 543, 969
Esp3I CGTCTC 2 cut(s) 150, 175
FalI AAGNNNNNCTT 2 cut(s) 1836, 1868
FaqI GGGAC 7 cut(s) 73, 97, 121, 145, 1111, 1479, 2026
FauNDI CATATG 1 cut(s) 997
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 2 cut(s) 975, 2239
FokI GGATG 4 cut(s) 763, 1135, 1741, 2183
FspBI CTAG 4 cut(s) 786, 915, 1788, 2138
GlaI GCGC 5 cut(s) 18, 534, 1184, 2086, 2115
GsuI CTGGAG 1 cut(s) 1658
HaeII RGCGCY 3 cut(s) 536, 1186, 2117
HaeIII GGCC 8 cut(s) 10, 24, 245, 392, 745, 838, 1119, 1124
HgaI GACGC 6 cut(s) 70, 94, 118, 142, 167, 192
HhaI GCGC 5 cut(s) 19, 535, 1185, 2087, 2116
Hin6I GCGC 5 cut(s) 17, 533, 1183, 2085, 2114
HinP1I GCGC 5 cut(s) 17, 533, 1183, 2085, 2114
HincII GTYRAC 1 cut(s) 2240
HindII GTYRAC 1 cut(s) 2240
HindIII AAGCTT 3 cut(s) 328, 715, 1759
HinfI GANTC 6 cut(s) 589, 653, 682, 863, 1781, 2165
HphI GGTGA 8 cut(s) 532, 691, 1049, 1063, 1385, 1591, 1730, 2174
Hpy166II GTNNAC 5 cut(s) 435, 912, 976, 1850, 2240
Hpy188III TCNNGA 8 cut(s) 58, 82, 106, 130, 334, 1411, 1872, 1972
Hpy8I GTNNAC 5 cut(s) 435, 912, 976, 1850, 2240
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 6 cut(s) 218, 256, 534, 1538, 1826, 2120
HpyCH4III ACNGT 8 cut(s) 360, 487, 562, 794, 1027, 1043, 1817, 1999
HpyCH4IV ACGT 6 cut(s) 437, 690, 1100, 1618, 2017, 2413
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 25, 192, 1307, 1341
HpyF3I CTNAG 2 cut(s) 740, 2045
HpySE526I ACGT 6 cut(s) 437, 690, 1100, 1618, 2017, 2413
HspAI GCGC 5 cut(s) 17, 533, 1183, 2085, 2114
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
LmnI GCTCC 3 cut(s) 47, 194, 1190
LweI GCATC 9 cut(s) 26, 757, 1280, 1347, 1423, 1502, 1781, 2022, 2205
MaeI CTAG 4 cut(s) 786, 915, 1788, 2138
MaeII ACGT 6 cut(s) 437, 690, 1100, 1618, 2017, 2413
MaeIII GTNAC 4 cut(s) 1019, 1037, 1579, 2286
MalI GATC 6 cut(s) 299, 903, 1077, 2082, 2151, 2181
MboI GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
MboII GAAGA 6 cut(s) 45, 948, 1307, 1733, 2014, 2067
MfeI CAATTG 2 cut(s) 878, 1302
MflI RGATCY 2 cut(s) 901, 2149
MmeI TCCRAC 1 cut(s) 2177
Mph1103I ATGCAT 3 cut(s) 1273, 1495, 2110
MseI TTAA 4 cut(s) 378, 1475, 1644, 2416
MslI CAYNNNNRTG 2 cut(s) 293, 1279
MspA1I CMGCKG 2 cut(s) 353, 1310
MunI CAATTG 2 cut(s) 878, 1302
Mva1269I GAATGC 2 cut(s) 288, 1710
MvaI CCWGG 4 cut(s) 384, 834, 840, 1394
MvnI CGCG 1 cut(s) 2085
MwoI GCNNNNNNNGC 5 cut(s) 16, 25, 192, 1307, 1341
NciI CCSGG 8 cut(s) 69, 93, 117, 141, 166, 747, 1121, 1539
NdeI CATATG 1 cut(s) 997
NdeII GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
NlaIV GGNNCC 2 cut(s) 229, 2151
NmuCI GTSAC 3 cut(s) 1037, 1579, 2286
NsiI ATGCAT 3 cut(s) 1273, 1495, 2110
NspI RCATGY 7 cut(s) 148, 173, 199, 376, 1213, 1750, 2287
OliI CACNNNNGTG 1 cut(s) 1279
PaeR7I CTCGAG 1 cut(s) 403
PagI TCATGA 1 cut(s) 1410
PaqCI CACCTGC 1 cut(s) 1361
PasI CCCWGGG 1 cut(s) 833
PceI AGGCCT 1 cut(s) 24
PciI ACATGT 1 cut(s) 1209
PctI GAATGC 2 cut(s) 288, 1710
PfeI GAWTC 6 cut(s) 589, 653, 682, 863, 1781, 2165
PflMI CCANNNNNTGG 1 cut(s) 2159
PfoI TCCNGGA 1 cut(s) 1392
Ple19I CGATCG 1 cut(s) 1078
PscI ACATGT 1 cut(s) 1209
PsiI TTATAA 2 cut(s) 1059, 1566
Psp1406I AACGTT 2 cut(s) 690, 1618
Psp6I CCWGG 4 cut(s) 382, 832, 838, 1392
PspGI CCWGG 4 cut(s) 382, 832, 838, 1392
PspN4I GGNNCC 2 cut(s) 229, 2151
PspPI GGNCC 6 cut(s) 243, 391, 725, 836, 1122, 1556
PspXI VCTCGAGB 1 cut(s) 403
PstNI CAGNNNCTG 2 cut(s) 656, 1341
PsuI RGATCY 2 cut(s) 901, 2149
PvuI CGATCG 1 cut(s) 1078
PvuII CAGCTG 2 cut(s) 353, 1310
RsaI GTAC 3 cut(s) 628, 1745, 2298
RsaNI GTAC 3 cut(s) 627, 1744, 2297
RseI CAYNNNNRTG 2 cut(s) 293, 1279
SalI GTCGAC 1 cut(s) 2238
SaqAI TTAA 4 cut(s) 378, 1475, 1644, 2416
Sau3AI GATC 6 cut(s) 297, 901, 1075, 2080, 2149, 2179
Sau96I GGNCC 6 cut(s) 243, 391, 725, 836, 1122, 1556
SfaNI GCATC 9 cut(s) 26, 757, 1280, 1347, 1423, 1502, 1781, 2022, 2205
SfcI CTRYAG 1 cut(s) 2346
Sfr274I CTCGAG 1 cut(s) 403
SinI GGWCC 2 cut(s) 725, 1556
SlaI CTCGAG 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 293, 1279
SmlI CTYRAG 3 cut(s) 325, 403, 1842
SmoI CTYRAG 3 cut(s) 325, 403, 1842
SseBI AGGCCT 1 cut(s) 24
SsiI CCGC 2 cut(s) 420, 2049
SspI AATATT 1 cut(s) 1868
SspMI CTAG 4 cut(s) 786, 915, 1788, 2138
StuI AGGCCT 1 cut(s) 24
StyI CCWWGG 2 cut(s) 543, 969
TaaI ACNGT 8 cut(s) 360, 487, 562, 794, 1027, 1043, 1817, 1999
TaiI ACGT 6 cut(s) 440, 693, 1103, 1621, 2020, 2416
TaqI TCGA 5 cut(s) 404, 425, 800, 1249, 2239
TatI WGTACW 1 cut(s) 1743
TfiI GAWTC 6 cut(s) 589, 653, 682, 863, 1781, 2165
Tru1I TTAA 4 cut(s) 378, 1475, 1644, 2416
Tru9I TTAA 4 cut(s) 378, 1475, 1644, 2416
TscAI CASTG 5 cut(s) 363, 1030, 1159, 1605, 2295
TseFI GTSAC 3 cut(s) 1037, 1579, 2286
Tsp45I GTSAC 3 cut(s) 1037, 1579, 2286
TspGWI ACGGA 3 cut(s) 693, 947, 1078
TspRI CASTG 5 cut(s) 363, 1030, 1159, 1605, 2295
Van91I CCANNNNNTGG 1 cut(s) 2159
VpaK11BI GGWCC 2 cut(s) 725, 1556
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 3 cut(s) 1067, 1240, 1941
XceI RCATGY 7 cut(s) 148, 173, 199, 376, 1213, 1750, 2287
XcmI CCANNNNNNNNNTGG 1 cut(s) 1112
XhoI CTCGAG 1 cut(s) 403
XmiI GTMKAC 2 cut(s) 975, 2239
XspI CTAG 4 cut(s) 786, 915, 1788, 2138
Zsp2I ATGCAT 3 cut(s) 1273, 1495, 2110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.