Rh5BG177900

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000096
Physical Location & Seq
Forward (+)
19307798 .. 19310976
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG177900.1

Sequence Viewer

Length: 2400 bp
ATGATCAACTTATTGTGCTTGCTCATCAGTTTCTCAAGCTTCTGGACTTGTCATGGTGCAGCTGCACTGTTCATTGGAGACACGCTTAATCCAGGGGAGACCCTCAATTCCTCGAGCTTTATGGTCTCCGCTTCGGGGGAGTTTACGTTGGGTTTCAACTCCGGCTACCTCATTCTGAGGTGCAAGAACTATACGGTTTGGGTTGCCAACCTGCACACTCCGATTTCAGACCCTTCGTCGGCGCTTCTCACCTTGGACAAGAATAAAACTGTCCTGCAAATTACCCTGAAAGGCAAGAATCCCATCGTTCTTTACTCCTCTTTTGATGATAATAGTACCAATGTCGTGGCTACCCTGTTAGATTCTGGCAATTTAGTCCTACGAGAGGTGAATCAGAAAGTTTCAAACTCTCCGTTGTGGCAAAGCTTTGATGGTCCTAATGTGGCTCTTAGGCCGGGGATGAAATTAGGTTTTGATGATGGTCTAACCGGCAACACTATGTCGATTTGGAGTTGGCGGGAAAGTCAGAACAGCCCGGGGCCAGGGTCTTTCAGCCTTTATTGGGATTCAGGCCAATTGCAAGTTAGAAGAAACGGGGATCTTTGGTGGACCAGCGAATATTACGAGTTGGAATCAAAGGTCTACAATGTTAGCGTGGTTTCATATGAGAATGGAGACTACTTCAGTTACACAGTCGTTGGTAATCCAAATAGCAGATCCGCCTGGGTGCTAGACACAGGCGGGAAGCTTTATGATGTTTACTATAACATTTCTATTGCGTCACCAGGTGACTGTGATGGCTATAACACCACCAATGATGGTGGTGGGCAGTGCCAGAGATGGAGTGGCCGGGCCACTCTTTATTGTCCTGTGGCAACATCCTATGGTGCACATATTCTGCAGATAGAAGGGCTCTCCTTTAGACCAATCAGTATGAACATGTCTATCAATTCAACCTGGGAGGAGACAAATTCAAGTCGATATGGGATTGATGAATGTATGGCTACTTGTTGGATGGACTGTGACTGCCTTGGCTTCGAATTTCTCTTTGATAATCAGACTGGATGTCGATTTTGGAGTGTAAAGTATTCTCAGGTCCTTGAAGATCCGGATAGTTCTGCAACTAAATATATTATACCAGGACTAGTCCCTACGTATAAACCGCAATCTCGAAGAAACATAATTGATGCATCACATAAGTGGATATGGATTGGTATTGCAATTGCGGCTGTTCTTCTGGCGCTGGTGCTTTGCAGCATCTGCTACCTAATACGAAAAAGAAAACTTGCAGGTGAGAACCAAACAAATATCCAGGATATGCTGAACATCATGAAATCTAATAGACCTACTGATGCTAATGGGCTTCAAAATGATGGAAAGAGGGGACAAGATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACTTGCAACTTCTCTAATGAAAATAAGCTCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGGAAATTGGTGACTGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAACGTTCAGGGCAGGGAATATATGAGTTTAAGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTTCAGCTGTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAAAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAGTTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAATATTCCAGAATGAAAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACAAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACACGTGGATATATGTCCCCTGAGTATATTATGGGGGGGAAATTTTCCATAAAATCTGATGTTTATAGTTTCGGTGTGTTGATGCTTGAAATCCTAAGTGGTAGAAGAAACAACAGCTTCTACAATGATGATCGCGCCCACAATTTAGTTGGTTATGCATGGGCGTTATGGAAAGAAGGTGCAGGACTAGAACTAATGGATCCAACACTTGGCGATTCATGTGATAAAGATCATTTGTTGAGGTGCATCCATGTTGGTTTGCTTTGCGTAGAGGAAAATGCAGCCAGTCGACCAAAGATGTTAGATGTGATATCTATGCTGACTAATGAAAGCATGTCACTGCCAGCACCTACAAAACCAGCATTTTGTACAGAAAGAAATGTCATCACAACTACTGTACGTGGAAACGGACCGGAAGTGGTGGTATCGGTAAATGGTATCTCCAATTCTGATTTTGATGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

799

Amino Acids

89.23

Weight (kDa)

5.56

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 65 - 173 3.4e-19 D-mannose binding lectin
Pkinase PF00069 481 - 681 1.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 483 - 681 1.7e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1485
AarI CACCTGC 1 cut(s) 1280
Acc36I ACCTGC 2 cut(s) 219, 1280
AccB7I CCANNNNNTGG 1 cut(s) 2141
AccI GTMKAC 2 cut(s) 642, 2221
AccII CGCG 1 cut(s) 2067
AccIII TCCGGA 1 cut(s) 1108
AciI CCGC 6 cut(s) 129, 517, 720, 741, 1163, 1226
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 5 cut(s) 606, 711, 1100, 2126, 2139
AcoI YGGCCR 1 cut(s) 847
AcsI RAATTY 4 cut(s) 970, 1040, 1860, 1973
AcuI CTGAAG 1 cut(s) 667
AcvI CACGTG 1 cut(s) 1937
AdeI CACNNNGTG 1 cut(s) 788
AfaI GTAC 4 cut(s) 337, 1664, 2302, 2331
AfiI CCNNNNNNNGG 6 cut(s) 135, 238, 385, 542, 562, 2141
AflIII ACRYGT 2 cut(s) 939, 1934
AhdI GACNNNNNGTC 2 cut(s) 235, 1065
AhlI ACTAGT 1 cut(s) 1144
AjnI CCWGG 7 cut(s) 91, 541, 722, 784, 956, 1138, 1311
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
AleI CACNNNNGTG 1 cut(s) 1198
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 5 cut(s) 72, 92, 130, 669, 959
Alw44I GTGCAC 1 cut(s) 888
AlwI GGATC 5 cut(s) 606, 711, 1100, 2126, 2139
AlwNI CAGNNNCTG 1 cut(s) 1260
Ama87I CYCGRG 2 cut(s) 112, 535
Aor13HI TCCGGA 1 cut(s) 1108
AoxI GGCC 5 cut(s) 452, 539, 571, 847, 852
ApaLI GTGCAC 1 cut(s) 888
ApeKI GCWGC 5 cut(s) 59, 62, 1254, 1424, 2213
ApoI RAATTY 4 cut(s) 970, 1040, 1860, 1973
AspLEI GCGC 3 cut(s) 244, 1243, 2069
AspS9I GGNCC 7 cut(s) 434, 539, 609, 852, 1096, 1475, 2342
AsuC2I CCSGG 4 cut(s) 456, 536, 537, 851
AsuHPI GGTGA 7 cut(s) 241, 400, 774, 800, 1304, 1510, 1649
AsuII TTCGAA 1 cut(s) 1038
AvaI CYCGRG 2 cut(s) 112, 535
AvaII GGWCC 5 cut(s) 434, 609, 1096, 1475, 2342
BaeGI GKGCMC 1 cut(s) 892
BamHI GGATCC 1 cut(s) 2131
BanII GRGCYC 1 cut(s) 915
BbrPI CACGTG 1 cut(s) 1937
Bbv12I GWGCWC 1 cut(s) 892
BbvI GCAGC 5 cut(s) 49, 71, 1266, 1411, 2225
BccI CCATC 9 cut(s) 311, 425, 473, 791, 812, 834, 1009, 1367, 2384
BciT130I CCWGG 7 cut(s) 93, 543, 724, 786, 958, 1140, 1313
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 456, 536, 537, 851
BcoDI GTCTC 5 cut(s) 72, 92, 130, 669, 959
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 4 cut(s) 731, 1145, 1707, 2120
BfmI CTRYAG 1 cut(s) 899
BfoI RGCGCY 2 cut(s) 245, 1244
BfuAI ACCTGC 2 cut(s) 219, 1280
BisI GCNGC 6 cut(s) 60, 63, 1227, 1255, 1425, 2214
BlsI GCNGC 6 cut(s) 61, 64, 1228, 1256, 1426, 2215
Bme18I GGWCC 5 cut(s) 434, 609, 1096, 1475, 2342
BmeRI GACNNNNNGTC 2 cut(s) 235, 1065
BmeT110I CYCGRG 2 cut(s) 112, 535
BmgT120I GGNCC 7 cut(s) 434, 539, 609, 852, 1096, 1475, 2342
BmiI GGNNCC 2 cut(s) 540, 2133
BmsI GCATC 8 cut(s) 1177, 1199, 1266, 1342, 1421, 1700, 2004, 2187
BpmI CTGGAG 1 cut(s) 1577
Bpu14I TTCGAA 1 cut(s) 1038
BpuEI CTTGAG 2 cut(s) 19, 1746
BpuMI CCSGG 4 cut(s) 456, 536, 537, 851
BsaAI YACGTR 3 cut(s) 1155, 1937, 2333
BsaBI GATNNNNATC 1 cut(s) 2160
BsaI GGTCTC 2 cut(s) 92, 130
BsaJI CCNNGG 9 cut(s) 92, 252, 455, 535, 536, 542, 723, 957, 1030
BsaWI WCCGGW 2 cut(s) 1108, 2344
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 238, 385, 542, 562, 2141
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 4 cut(s) 1066, 1507, 1730, 2217
Bse8I GATNNNNATC 1 cut(s) 2160
BseAI TCCGGA 1 cut(s) 1108
BseBI CCWGG 7 cut(s) 93, 543, 724, 786, 958, 1140, 1313
BseDI CCNNGG 9 cut(s) 92, 252, 455, 535, 536, 542, 723, 957, 1030
BseGI GGATG 6 cut(s) 465, 878, 1020, 1070, 1653, 2178
BseJI GATNNNNATC 1 cut(s) 2160
BseLI CCNNNNNNNGG 6 cut(s) 135, 238, 385, 542, 562, 2141
BseMII CTCAG 3 cut(s) 167, 1106, 1944
BseNI ACTGG 4 cut(s) 1066, 1507, 1730, 2217
BseRI GAGGAG 2 cut(s) 307, 977
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 5 cut(s) 49, 71, 1266, 1411, 2225
BsgI GTGCAG 4 cut(s) 48, 78, 197, 2133
Bsh1236I CGCG 1 cut(s) 2067
BshFI GGCC 5 cut(s) 454, 541, 573, 849, 854
BsiHKAI GWGCWC 1 cut(s) 892
BsiHKCI CYCGRG 2 cut(s) 112, 535
BsiSI CCGG 7 cut(s) 162, 455, 489, 536, 850, 1109, 2345
BslFI GGGAC 4 cut(s) 1133, 1398, 1933, 1945
BslI CCNNNNNNNGG 6 cut(s) 135, 238, 385, 542, 562, 2141
BsmAI GTCTC 5 cut(s) 72, 92, 130, 669, 959
BsmFI GGGAC 4 cut(s) 1133, 1398, 1933, 1945
BsmI GAATGC 1 cut(s) 1629
BsnI GGCC 5 cut(s) 454, 541, 573, 849, 854
Bso31I GGTCTC 2 cut(s) 92, 130
BsoBI CYCGRG 2 cut(s) 112, 535
Bsp119I TTCGAA 1 cut(s) 1038
Bsp1286I GDGCHC 2 cut(s) 892, 915
Bsp13I TCCGGA 1 cut(s) 1108
Bsp1407I TGTACA 1 cut(s) 2300
Bsp143I GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
BspACI CCGC 6 cut(s) 129, 517, 720, 741, 1163, 1226
BspANI GGCC 5 cut(s) 454, 541, 573, 849, 854
BspCNI CTCAG 3 cut(s) 168, 1105, 1945
BspEI TCCGGA 1 cut(s) 1108
BspFNI CGCG 1 cut(s) 2067
BspHI TCATGA 1 cut(s) 1329
BspLI GGNNCC 2 cut(s) 540, 2133
BspMAI CTGCAG 1 cut(s) 903
BspMI ACCTGC 2 cut(s) 219, 1280
BspPI GGATC 5 cut(s) 606, 711, 1100, 2126, 2139
BspT104I TTCGAA 1 cut(s) 1038
BspTNI GGTCTC 2 cut(s) 92, 130
BsrFI RCCGGY 1 cut(s) 488
BsrGI TGTACA 1 cut(s) 2300
BsrI ACTGG 4 cut(s) 1066, 1507, 1730, 2217
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 9 cut(s) 92, 252, 455, 535, 536, 542, 723, 957, 1030
BssMI GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
BssT1I CCWWGG 2 cut(s) 252, 1030
Bst2UI CCWGG 7 cut(s) 93, 543, 724, 786, 958, 1140, 1313
Bst4CI ACNGT 8 cut(s) 69, 196, 271, 694, 794, 1022, 1736, 2329
Bst6I CTCTTC 1 cut(s) 1635
BstAPI GCANNNNNTGC 1 cut(s) 1260
BstAUI TGTACA 1 cut(s) 2300
BstBAI YACGTR 3 cut(s) 1155, 1937, 2333
BstBI TTCGAA 1 cut(s) 1038
BstC8I GCNNGC 3 cut(s) 20, 1715, 2277
BstDEI CTNAG 5 cut(s) 176, 449, 1092, 1953, 2027
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 6 cut(s) 465, 878, 1020, 1070, 1653, 2178
BstFNI CGCG 1 cut(s) 2067
BstH2I RGCGCY 2 cut(s) 245, 1244
BstHHI GCGC 3 cut(s) 244, 1243, 2069
BstKTI GATC 7 cut(s) 6, 601, 719, 1108, 2065, 2134, 2164
BstMAI GTCTC 5 cut(s) 72, 92, 130, 669, 959
BstMBI GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
BstMWI GCNNNNNNNGC 2 cut(s) 1226, 1260
BstNI CCWGG 7 cut(s) 93, 543, 724, 786, 958, 1140, 1313
BstNSI RCATGY 3 cut(s) 943, 1669, 2269
BstSFI CTRYAG 1 cut(s) 899
BstSLI GKGCMC 1 cut(s) 892
BstSNI TACGTA 1 cut(s) 1155
BstUI CGCG 1 cut(s) 2067
BstV1I GCAGC 5 cut(s) 49, 71, 1266, 1411, 2225
BstX2I RGATCY 4 cut(s) 598, 716, 1105, 2131
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 4 cut(s) 598, 716, 1105, 2131
BsuRI GGCC 5 cut(s) 454, 541, 573, 849, 854
BtsCI GGATG 6 cut(s) 465, 878, 1020, 1070, 1653, 2178
BtsI GCAGTG 3 cut(s) 836, 1524, 2270
BtsIMutI CAGTG 4 cut(s) 65, 836, 1524, 2270
BveI ACCTGC 2 cut(s) 219, 1280
Cac8I GCNNGC 3 cut(s) 20, 1715, 2277
CaiI CAGNNNCTG 1 cut(s) 1260
CciI TCATGA 1 cut(s) 1329
CfoI GCGC 3 cut(s) 244, 1243, 2069
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 7 cut(s) 434, 539, 609, 852, 1096, 1475, 2342
Cfr9I CCCGGG 1 cut(s) 535
CpoI CGGWCCG 1 cut(s) 2342
CseI GACGC 1 cut(s) 768
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 4 cut(s) 336, 1663, 2301, 2330
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CspI CGGWCCG 1 cut(s) 2342
CviQI GTAC 4 cut(s) 336, 1663, 2301, 2330
DdeI CTNAG 5 cut(s) 176, 449, 1092, 1953, 2027
DpnI GATC 7 cut(s) 5, 600, 718, 1107, 2064, 2133, 2163
DpnII GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
DraIII CACNNNGTG 1 cut(s) 788
DriI GACNNNNNGTC 2 cut(s) 235, 1065
EaeI YGGCCR 1 cut(s) 847
Eam1104I CTCTTC 1 cut(s) 1635
Eam1105I GACNNNNNGTC 2 cut(s) 235, 1065
EarI CTCTTC 1 cut(s) 1635
EciI GGCGGA 1 cut(s) 709
Eco105I TACGTA 1 cut(s) 1155
Eco130I CCWWGG 2 cut(s) 252, 1030
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 2 cut(s) 92, 130
Eco32I GATATC 1 cut(s) 2244
Eco47I GGWCC 5 cut(s) 434, 609, 1096, 1475, 2342
Eco57I CTGAAG 1 cut(s) 667
Eco72I CACGTG 1 cut(s) 1937
Eco88I CYCGRG 2 cut(s) 112, 535
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 1096
EcoRII CCWGG 7 cut(s) 91, 541, 722, 784, 956, 1138, 1311
EcoRV GATATC 1 cut(s) 2244
EcoT14I CCWWGG 2 cut(s) 252, 1030
EcoT22I ATGCAT 3 cut(s) 1192, 1414, 2092
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 252, 1030
FalI AAGNNNNNCTT 2 cut(s) 1755, 1787
FaqI GGGAC 4 cut(s) 1133, 1398, 1933, 1945
FauI CCCGC 2 cut(s) 510, 734
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 642, 2221
Fnu4HI GCNGC 6 cut(s) 60, 63, 1227, 1255, 1425, 2214
FokI GGATG 6 cut(s) 472, 865, 1027, 1077, 1660, 2165
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 6 cut(s) 60, 63, 1227, 1255, 1425, 2214
FspBI CTAG 4 cut(s) 731, 1145, 1707, 2120
GlaI GCGC 3 cut(s) 243, 1242, 2068
GluI GCNGC 6 cut(s) 60, 63, 1227, 1255, 1425, 2214
GsuI CTGGAG 1 cut(s) 1577
HaeII RGCGCY 2 cut(s) 245, 1244
HaeIII GGCC 5 cut(s) 454, 541, 573, 849, 854
HapII CCGG 7 cut(s) 162, 455, 489, 536, 850, 1109, 2345
HgaI GACGC 1 cut(s) 768
HhaI GCGC 3 cut(s) 244, 1243, 2069
Hin6I GCGC 3 cut(s) 242, 1241, 2067
HinP1I GCGC 3 cut(s) 242, 1241, 2067
HincII GTYRAC 1 cut(s) 2222
HindII GTYRAC 1 cut(s) 2222
HindIII AAGCTT 4 cut(s) 37, 424, 746, 1678
HinfI GANTC 7 cut(s) 298, 362, 391, 566, 632, 1700, 2147
HpaII CCGG 7 cut(s) 162, 455, 489, 536, 850, 1109, 2345
HphI GGTGA 7 cut(s) 241, 400, 774, 800, 1304, 1510, 1649
Hpy166II GTNNAC 7 cut(s) 144, 609, 643, 760, 890, 1769, 2222
Hpy188III TCNNGA 5 cut(s) 43, 1109, 1170, 1330, 1791
Hpy8I GTNNAC 7 cut(s) 144, 609, 643, 760, 890, 1769, 2222
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 243, 902, 1457, 1745, 2102
HpyCH4III ACNGT 8 cut(s) 69, 196, 271, 694, 794, 1022, 1736, 2329
HpyCH4IV ACGT 6 cut(s) 146, 1154, 1537, 1936, 2332, 2395
HpyF10VI GCNNNNNNNGC 2 cut(s) 1226, 1260
HpyF3I CTNAG 5 cut(s) 176, 449, 1092, 1953, 2027
HpySE526I ACGT 6 cut(s) 146, 1154, 1537, 1936, 2332, 2395
HspAI GCGC 3 cut(s) 242, 1241, 2067
Kpn2I TCCGGA 1 cut(s) 1108
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
Lsp1109I GCAGC 5 cut(s) 49, 71, 1266, 1411, 2225
LweI GCATC 8 cut(s) 1177, 1199, 1266, 1342, 1421, 1700, 2004, 2187
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 4 cut(s) 731, 1145, 1707, 2120
MaeII ACGT 6 cut(s) 146, 1154, 1537, 1936, 2332, 2395
MaeIII GTNAC 6 cut(s) 686, 780, 788, 1022, 1498, 2268
MalI GATC 7 cut(s) 5, 600, 718, 1107, 2064, 2133, 2163
MboI GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
MboII GAAGA 7 cut(s) 600, 1115, 1185, 1226, 1652, 1933, 2049
MfeI CAATTG 2 cut(s) 575, 1221
MflI RGATCY 4 cut(s) 598, 716, 1105, 2131
MhlI GDGCHC 2 cut(s) 892, 915
MmeI TCCRAC 3 cut(s) 609, 992, 2159
Mph1103I ATGCAT 3 cut(s) 1192, 1414, 2092
MroI TCCGGA 1 cut(s) 1108
MseI TTAA 4 cut(s) 87, 1394, 1563, 2398
MslI CAYNNNNRTG 1 cut(s) 1198
MspA1I CMGCKG 2 cut(s) 62, 1615
MspI CCGG 7 cut(s) 162, 455, 489, 536, 850, 1109, 2345
MunI CAATTG 2 cut(s) 575, 1221
Mva1269I GAATGC 1 cut(s) 1629
MvaI CCWGG 7 cut(s) 93, 543, 724, 786, 958, 1140, 1313
MvnI CGCG 1 cut(s) 2067
MwoI GCNNNNNNNGC 2 cut(s) 1226, 1260
NciI CCSGG 4 cut(s) 456, 536, 537, 851
NdeI CATATG 1 cut(s) 664
NdeII GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
NlaIV GGNNCC 2 cut(s) 540, 2133
NmuCI GTSAC 5 cut(s) 780, 788, 1022, 1498, 2268
NsiI ATGCAT 3 cut(s) 1192, 1414, 2092
NspI RCATGY 3 cut(s) 943, 1669, 2269
NspV TTCGAA 1 cut(s) 1038
OliI CACNNNNGTG 1 cut(s) 1198
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 1 cut(s) 1329
PaqCI CACCTGC 1 cut(s) 1280
PciI ACATGT 1 cut(s) 939
PctI GAATGC 1 cut(s) 1629
PfeI GAWTC 7 cut(s) 298, 362, 391, 566, 632, 1700, 2147
PflMI CCANNNNNTGG 1 cut(s) 2141
PfoI TCCNGGA 1 cut(s) 1311
PkrI GCNGC 6 cut(s) 61, 64, 1228, 1256, 1426, 2215
PmaCI CACGTG 1 cut(s) 1937
PmlI CACGTG 1 cut(s) 1937
Ppu21I YACGTR 3 cut(s) 1155, 1937, 2333
PpuMI RGGWCCY 1 cut(s) 1096
PscI ACATGT 1 cut(s) 939
PsiI TTATAA 1 cut(s) 1485
Psp1406I AACGTT 1 cut(s) 1537
Psp5II RGGWCCY 1 cut(s) 1096
Psp6I CCWGG 7 cut(s) 91, 541, 722, 784, 956, 1138, 1311
PspCI CACGTG 1 cut(s) 1937
PspGI CCWGG 7 cut(s) 91, 541, 722, 784, 956, 1138, 1311
PspN4I GGNNCC 2 cut(s) 540, 2133
PspPI GGNCC 7 cut(s) 434, 539, 609, 852, 1096, 1475, 2342
PspPPI RGGWCCY 1 cut(s) 1096
PspXI VCTCGAGB 1 cut(s) 112
PstI CTGCAG 1 cut(s) 903
PstNI CAGNNNCTG 1 cut(s) 1260
PsuI RGATCY 4 cut(s) 598, 716, 1105, 2131
PvuII CAGCTG 2 cut(s) 62, 1615
RsaI GTAC 4 cut(s) 337, 1664, 2302, 2331
RsaNI GTAC 4 cut(s) 336, 1663, 2301, 2330
RseI CAYNNNNRTG 1 cut(s) 1198
Rsr2I CGGWCCG 1 cut(s) 2342
RsrII CGGWCCG 1 cut(s) 2342
SalI GTCGAC 1 cut(s) 2220
SaqAI TTAA 4 cut(s) 87, 1394, 1563, 2398
SatI GCNGC 6 cut(s) 60, 63, 1227, 1255, 1425, 2214
Sau3AI GATC 7 cut(s) 3, 598, 716, 1105, 2062, 2131, 2161
Sau96I GGNCC 7 cut(s) 434, 539, 609, 852, 1096, 1475, 2342
SduI GDGCHC 2 cut(s) 892, 915
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 8 cut(s) 1177, 1199, 1266, 1342, 1421, 1700, 2004, 2187
SfcI CTRYAG 1 cut(s) 899
Sfr274I CTCGAG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 1038
SinI GGWCC 5 cut(s) 434, 609, 1096, 1475, 2342
SlaI CTCGAG 1 cut(s) 112
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 1198
SmlI CTYRAG 3 cut(s) 34, 112, 1761
SmoI CTYRAG 3 cut(s) 34, 112, 1761
SnaBI TACGTA 1 cut(s) 1155
SpeI ACTAGT 1 cut(s) 1144
SsiI CCGC 6 cut(s) 129, 517, 720, 741, 1163, 1226
SspI AATATT 2 cut(s) 620, 1787
SspMI CTAG 4 cut(s) 731, 1145, 1707, 2120
StyI CCWWGG 2 cut(s) 252, 1030
TaaI ACNGT 8 cut(s) 69, 196, 271, 694, 794, 1022, 1736, 2329
TaiI ACGT 6 cut(s) 149, 1157, 1540, 1939, 2335, 2398
TaqI TCGA 7 cut(s) 113, 503, 979, 1038, 1069, 1171, 2221
TatI WGTACW 2 cut(s) 1662, 2300
TauI GCSGC 1 cut(s) 1229
TfiI GAWTC 7 cut(s) 298, 362, 391, 566, 632, 1700, 2147
Tru1I TTAA 4 cut(s) 87, 1394, 1563, 2398
Tru9I TTAA 4 cut(s) 87, 1394, 1563, 2398
TscAI CASTG 4 cut(s) 72, 836, 1524, 2277
TseFI GTSAC 5 cut(s) 780, 788, 1022, 1498, 2268
TseI GCWGC 5 cut(s) 59, 62, 1254, 1424, 2213
Tsp45I GTSAC 5 cut(s) 780, 788, 1022, 1498, 2268
TspGWI ACGGA 2 cut(s) 402, 2355
TspMI CCCGGG 1 cut(s) 535
TspRI CASTG 4 cut(s) 72, 836, 1524, 2277
Van91I CCANNNNNTGG 1 cut(s) 2141
VneI GTGCAC 1 cut(s) 888
VpaK11BI GGWCC 5 cut(s) 434, 609, 1096, 1475, 2342
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 4 cut(s) 970, 1040, 1860, 1973
XceI RCATGY 3 cut(s) 943, 1669, 2269
XcmI CCANNNNNNNNNTGG 2 cut(s) 842, 2078
XhoI CTCGAG 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 535
XmiI GTMKAC 2 cut(s) 642, 2221
XspI CTAG 4 cut(s) 731, 1145, 1707, 2120
Zsp2I ATGCAT 3 cut(s) 1192, 1414, 2092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.