Rw5G016050

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000096
Physical Location & Seq
Forward (+)
20045921 .. 20048885
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016050.1

Sequence Viewer

Length: 2379 bp
ATGGCTGGCGGTGTCGATTTGATTTGCTTGGTGTTTATGTGGAGCTTGTGGAGTACTTGTGATGGTGCAACAAACATACTGAAACCAGGTGAAAGTCTAGATTCTTCAAGGTCATTAGTTTCTGCAAATCGGAAGTTCACTTTGAATTTTCGTGTTTATGAGCATGATGCAAACTTGAGTTACTTAGTTATAAAGTGGAATGCGAGTCATAACTATGCGTGGGTTGCGAATAGAGAAACACCCATTTTGTACCCTTTTGGAGTTCTTACATTGGACAGGAACTACACATTGAAGATTACTCACAGAGATGGGGCTGCTGTGGTGCTTTACTCTGCTGATTCGGAGACCATCAGTGGTGATGTTGTGGCTACTCTTATGGATGATGGGAATTTTGTGCTGCAAGAAGTGAGTGATGCTGGATCGGTGAAGAGGGATTTGTGGCAGAGTTTTGATTATCCTGGGGATGTGCTTCTGCCAGGTATGAAATTAGGGGTTAACCGTAGTAATGGGCATAATTGGTCACTTTCGTGCTGGTTAACTGAGAAAAGTGCAGTGCCAGGACCCTTCACTCTTGTTTGGGACCCTGATGGACACGACTTGAAAATTAAGCGGCGTGGGGTGGTGTATTGGAGTAGTGGAGTATTTCGCGATGGGAGTTTTGAAAATATTAAGCAGAAGAGGTATAATTTTAGCATTGTTTCAAAGAAGAATGAAGACTATTTTAGTTACACTACTTTAGATGAAAATGCTGTATCAGAGTGGCTGCTAACCACAATAGGGCGACTAAAAGACTTTGATGAATCAATTGATATTGCAAAAGCAGATTCCTGTTATGGGTATAACACTGACGGTGGATGCCAGATTTGGGACCAGCCAACTAAGTGTGTGCGTACTGGTGATGTATTTGAGCAAGAAAATGGTTACTTTAATCCAACAGATGCTAGCGGCTCTACTGTATCATCAAGAAGTGATTCAAATACTAGTCTCAGTATTAGTGATTGTAAGGATGCTTGTTGGGCAGATTGTAACTGTCGTGGATTCATCTTTCTGTTTCCTAATCAGACTGGATGCCGGTATTGGACTGGAAACTTGAAATTCATTGCAGACAGCGCAGGTTATAGTTCAAGTGTTGTATATTTTTTAACAACAAAGTCAGACAGCAACTCACATAAGTGGATATGGATTGGCGCTGCTATTGGCACTTCTGTATTTTTAATGGCAGTTTGCATCATCTGCTGTCTATTAAGAAGAAGAAAACTCTCCCTTTTTGGGAAGAACAAAGCAAAGATTGATGAGAAGGACCTGCTTGACTTAAGGGGATCTGATATATCAACTGATGTCCACGGGATTCAGAATGATGGATCGATGGGACATGATTTAAGAGCATTTAGCTATGAATCTATCATGGCTGCCACAGACTGCTTCTCCTTCCATAACAAGCTAGGAGAGGGGGGCTTTGGACCTGTTTATAAGGGAAAATTGTCATCAGGGCGAGAAGTAGCTATAAAGAGGCTTTCAAGAGGTTCGGTGCAGGGAACATTGGAGTTTAAAAATGAATTGATACTGATATCTGAACTCCAACATACTAACCTTGTTCAGCTCTTGGGATATTGCATTCATGGTGTAGAGAGGATGTTAATATACGAGTATCTGCCAAACAAAAGTTTAGACTACATCTTATTTGATTCAACCAGAGGCATGCTACTAGATTGGAAGAAGCGTTTCAATATAATTGAAGGAATTGCTCAAGGATTGATTTACTTGCACAAATACTCAAGATTGAAAGTAATTCATAGAGATCTTAAAGCTAGTAACATACTACTTGATGAAGATATGAATCCCAAAATTTCTGATTTTGGTTTGGCAAGGATTTTCACGGTTAATGAAGTTGAAGCAAATACTAATCGGATTGTTGGCACATATGGTTACATGTCTCCTGAGTATGCTATGGAGGGAATTTTTTCTGGCAAATCTGATGTCTTCAGTTTTGGAGTGTTGATGCTTGAAATCATAAGTGGTAGGAGAAACAACAGCTTCCACAATGCTCATCGCTCGCTCAATTTAGTAGGATATACATGGGAGTTATGGAAAGAAGGTGCAGGACTAGATTTAATGGATCCAAGGCTAAGCGATTCATGTAATAGAGATCAACTGCTAAGATGCATACATGTTGGTCTATTATGTGTGGAAGAAGATGCAGAACATCGGCCAACCATGTCAGATGCCATATCACTGTTGACAAATGAAAGCTTGCCATTACCTACACCAACAAAACCAGCATTTTTCCCAGTAAGACGTGTGGCTGAAGTTGATGTAAGTGGAAGCAGATCGGAAATTTCGTCAAATGACTTGTCAAACTCCTTTGTTATTGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

792

Amino Acids

88.74

Weight (kDa)

5.85

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 178 7.2e-24 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 476 - 741 5.7e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 476 - 683 5.3e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000096)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24770 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15080 FvH4_3g15090 FvH4_3g15090 FvH4_3g15090 FvH4_3g15100 FvH4_3g15110 FvH4_3g15110 FvH4_3g15120 FvH4_3g15120 FvH4_3g15130 FvH4_3g15140 FvH4_3g15150 FvH4_3g15160 FvH4_3g29142
malus_domestica MD05G1226400.v1.1 MD05G1227000.v1.1 MD05G1227200.v1.1 MD05G1227300.v1.1 MD05G1227400.v1.1 MD05G1227500.v1.1 MD05G1227600.v1.1 MD09G1060100.v1.1 MD10G1206700.v1.1 MD10G1206800.v1.1 MD10G1206900.v1.1 MD10G1207000.v1.1 MD10G1207300.v1.1 MD10G1207500.v1.1 MD10G1207700.v1.1 MD10G1207800.v1.1 MD10G1208000.v1.1
prunus_persica Prupe.4G134900_v2.0.a1 Prupe.4G135000_v2.0.a1 Prupe.4G135100_v2.0.a1 Prupe.4G135200_v2.0.a1 Prupe.4G135400_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135500_v2.0.a1 Prupe.4G135600_v2.0.a1 Prupe.4G162400_v2.0.a1 Prupe.6G158800_v2.0.a1 Prupe.6G158800_v2.0.a1
pyrus_communis pycom05g20870 pycom05g20880 pycom05g20950 pycom05g20960 pycom10g17650 pycom10g17670 pycom10g17690
rosa_chinensis RchiOBHm_Chr4g0410521 RchiOBHm_Chr5g0024831 RchiOBHm_Chr5g0024841 RchiOBHm_Chr5g0024871 RchiOBHm_Chr5g0024901 RchiOBHm_Chr5g0024921 RchiOBHm_Chr5g0024951 RchiOBHm_Chr5g0024971 RchiOBHm_Chr5g0025011 RchiOBHm_Chr5g0025031 RchiOBHm_Chr5g0025041 RchiOBHm_Chr5g0025051 RchiOBHm_Chr5g0025071 RchiOBHm_Chr5g0025091 RchiOBHm_Chr5g0025101 RchiOBHm_Chr5g0025121 RchiOBHm_Chr5g0025131 RchiOBHm_Chr5g0025171 RchiOBHm_Chr5g0025181 RchiOBHm_Chr5g0025201 RchiOBHm_Chr5g0025211 RchiOBHm_Chr5g0025221 RchiOBHm_Chr5g0025261 RchiOBHm_Chr5g0025321 RchiOBHm_Chr5g0025351 RchiOBHm_Chr5g0025371 RchiOBHm_Chr5g0025381 RchiOBHm_Chr5g0025421 RchiOBHm_Chr5g0025451 RchiOBHm_Chr5g0025511 RchiOBHm_Chr5g0025521 RchiOBHm_Chr5g0025531 RchiOBHm_Chr5g0025551 RchiOBHm_Chr5g0025571 RchiOBHm_Chr5g0025581 RchiOBHm_Chr5g0025611 RchiOBHm_Chr5g0072881
rosa_laevigata RLG00000008419 RLG00000032818 RLG00000032819 RLG00000032820 RLG00000032822 RLG00000032823 RLG00000032824 RLG00000032826 RLG00000032828 RLG00000032832 RLG00000032834 RLG00000032835 RLG00000032836 RLG00000032837 RLG00000032838 RLG00000032839 RLG00000032840 RLG00000032841 RLG00000032842 RLG00000032844 RLG00000032845 RLG00000032847 RLG00000032848 RLG00000032849
rosa_multiflora Rmu_co8009526.1_g000001 Rmu_co8228985.1_g000001 Rmu_co8395667.1_g000001 Rmu_sc0001069.1_g000006 Rmu_sc0001069.1_g000008 Rmu_sc0001069.1_g000030 Rmu_sc0001069.1_g000031 Rmu_sc0003576.1_g000001 Rmu_sc0003576.1_g000006 Rmu_sc0003576.1_g000011 Rmu_sc0003576.1_g000020 Rmu_sc0003576.1_g000021 Rmu_sc0003576.1_g000024 Rmu_sc0003576.1_g000028 Rmu_sc0004107.1_g000005 Rmu_sc0004107.1_g000006 Rmu_sc0004107.1_g000007 Rmu_sc0004107.1_g000017 Rmu_sc0005100.1_g000007 Rmu_sc0005564.1_g000005 Rmu_sc0006827.1_g000005 Rmu_sc0006827.1_g000006 Rmu_sc0006827.1_g000012 Rmu_sc0006827.1_g000014 Rmu_sc0006827.1_g000016 Rmu_sc0006827.1_g000020 Rmu_sc0006827.1_g000025 Rmu_sc0009744.1_g000005 Rmu_sc0018969.1_g000001 Rmu_sc0039632.1_g000001
rosa_roxburghii Rroxscaffold_1G00043620 Rroxscaffold_1G00053590 Rroxscaffold_1G00053620 Rroxscaffold_1G00053660 Rroxscaffold_1G00053670 Rroxscaffold_1G00053680 Rroxscaffold_1G00053740 Rroxscaffold_1G00053760 Rroxscaffold_1G00053770 Rroxscaffold_1G00053790 Rroxscaffold_1G00053810 Rroxscaffold_1G00053830 Rroxscaffold_1G00053840 Rroxscaffold_1G00053850 Rroxscaffold_1G00053860 Rroxscaffold_1G00053880 Rroxscaffold_1G00053890 Rroxscaffold_1G00053920 Rroxscaffold_1G00053930 Rroxscaffold_1G00053940 Rroxscaffold_1G00053950 Rroxscaffold_1G00053980 Rroxscaffold_1G00054000 Rroxscaffold_1G00054050 Rroxscaffold_1G00054080 Rroxscaffold_1G00054090 Rroxscaffold_1G00054110 Rroxscaffold_1G00054130 Rroxscaffold_1G00054160 Rroxscaffold_1G00054170 Rroxscaffold_1G00054390 Rroxscaffold_1G00054440 Rroxscaffold_1G00054450 Rroxscaffold_1G00054460 Rroxscaffold_1G00054480 Rroxscaffold_1G00054520 Rroxscaffold_1G00054540 Rroxscaffold_1G00054550 Rroxscaffold_7G00198640
rosa_rugosa Rorug05G0084800.1 Rorug05G0084900.1 Rorug05G0085100.1 Rorug05G0085200.1 Rorug05G0085300 Rorug05G0085300 Rorug05G0085400 Rorug05G0085400 Rorug05G0085500 Rorug05G0085600 Rorug05G0085700 Rorug05G0085800 Rorug05G0085900 Rorug05G0086000 Rorug05G0086400 Rorug05G0086600.1 Rorug05G0086900 Rorug05G0087000 Rorug05G0087100 Rorug05G0088000
rosa_samantha Rh4AG161200 Rh4BG160700 Rh4CG173500 Rh5AG173800 Rh5AG176700 Rh5AG176800 Rh5AG177000 Rh5AG177900 Rh5AG178900 Rh5BG174300 Rh5BG174400 Rh5BG174600 Rh5BG174700 Rh5BG174800 Rh5BG175000 Rh5BG175400 Rh5BG176000 Rh5BG176100 Rh5BG176200 Rh5BG176300 Rh5BG176600 Rh5BG176700 Rh5BG177000 Rh5BG177400 Rh5BG177900 Rh5CG191400 Rh5CG191600 Rh5CG191700 Rh5CG191900 Rh5CG192000 Rh5CG192200 Rh5CG192300 Rh5CG192500 Rh5CG192600 Rh5CG193200 Rh5CG193300 Rh5CG193400 Rh5CG193500 Rh5CG193700 Rh5CG193800 Rh5CG193900 Rh5CG194000 Rh5CG194100 Rh5CG194200 Rh5CG194600 Rh5CG194700 Rh5CG194800 Rh5CG195300 Rh5CG195400 Rh5CG195500 Rh5CG195600
rosa_wichuraiana Rw4G013480 Rw5G016010 Rw5G016020 Rw5G016030 Rw5G016040 Rw5G016050 Rw5G016110 Rw5G016120 Rw5G016130 Rw5G016140 Rw5G016150 Rw5G016170 Rw5G016180 Rw5G016230 Rw5G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 191, 1470
Acc36I ACCTGC 2 cut(s) 1103, 1311
AccII CGCG 1 cut(s) 648
AciI CCGC 3 cut(s) 9, 610, 945
AclWI GGATC 5 cut(s) 427, 1327, 1369, 2111, 2124
AcoI YGGCCR 1 cut(s) 2207
AcsI RAATTY 6 cut(s) 145, 388, 1094, 1845, 1956, 2334
AcuI CTGAAG 2 cut(s) 1966, 2325
AfaI GTAC 3 cut(s) 55, 251, 892
AfiI CCNNNNNNNGG 4 cut(s) 777, 834, 865, 1269
AflII CTTAAG 1 cut(s) 1312
AflIII ACRYGT 3 cut(s) 1929, 2167, 2296
AhlI ACTAGT 1 cut(s) 980
AjiI CACGTC 1 cut(s) 2297
AjnI CCWGG 4 cut(s) 85, 457, 475, 556
AleI CACNNNNGTG 2 cut(s) 526, 1171
AluBI AGCT 8 cut(s) 45, 1392, 1441, 1502, 1600, 1808, 2034, 2250
AluI AGCT 8 cut(s) 45, 1392, 1441, 1502, 1600, 1808, 2034, 2250
Alw26I GTCTC 3 cut(s) 338, 989, 1938
AlwI GGATC 5 cut(s) 427, 1327, 1369, 2111, 2124
AoxI GGCC 1 cut(s) 2207
ApeKI GCWGC 5 cut(s) 314, 397, 763, 1190, 1409
ApoI RAATTY 6 cut(s) 145, 388, 1094, 1845, 1956, 2334
ArsI GACNNNNNNTTYG 4 cut(s) 1962, 1994, 2336, 2368
Asp700I GAANNNNTTC 3 cut(s) 970, 1721, 1960
AspLEI GCGC 2 cut(s) 1112, 1190
AspS9I GGNCC 5 cut(s) 560, 580, 868, 1300, 1460
AsuHPI GGTGA 4 cut(s) 101, 368, 436, 908
AsuNHI GCTAGC 1 cut(s) 941
AvaII GGWCC 5 cut(s) 560, 580, 868, 1300, 1460
BamHI GGATCC 1 cut(s) 2116
BbsI GAAGAC 2 cut(s) 720, 1972
BbvI GCAGC 5 cut(s) 301, 384, 750, 1177, 1396
BccI CCATC 8 cut(s) 56, 302, 356, 377, 581, 644, 1352, 1360
BciT130I CCWGG 4 cut(s) 87, 459, 477, 558
BcoDI GTCTC 3 cut(s) 338, 989, 1938
BcuI ACTAGT 1 cut(s) 980
BfaI CTAG 7 cut(s) 98, 942, 981, 1442, 1706, 1809, 2105
BfoI RGCGCY 1 cut(s) 1191
BfrI CTTAAG 1 cut(s) 1312
BfuAI ACCTGC 2 cut(s) 1103, 1311
BglII AGATCT 1 cut(s) 1798
BisI GCNGC 7 cut(s) 315, 398, 611, 764, 946, 1191, 1410
BlpI GCTNAGC 1 cut(s) 2126
BlsI GCNGC 7 cut(s) 316, 399, 612, 765, 947, 1192, 1411
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 4 cut(s) 87, 459, 477, 558
Bme18I GGWCC 5 cut(s) 560, 580, 868, 1300, 1460
BmgBI CACGTC 1 cut(s) 2297
BmgT120I GGNCC 5 cut(s) 560, 580, 868, 1300, 1460
BmiI GGNNCC 5 cut(s) 562, 581, 582, 869, 2118
BmrFI CCNGG 4 cut(s) 87, 459, 477, 558
BmrI ACTGGG 1 cut(s) 2282
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 2282
BpiI GAAGAC 2 cut(s) 720, 1972
Bpu1102I GCTNAGC 1 cut(s) 2126
BpuEI CTTGAG 3 cut(s) 196, 1731, 1759
Bsa29I ATCGAT 1 cut(s) 1364
BsaBI GATNNNNATC 1 cut(s) 1836
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 458, 1342, 2120
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 1409, 1439
Bsc4I CCNNNNNNNGG 4 cut(s) 777, 834, 865, 1269
Bse118I RCCGGY 1 cut(s) 1071
Bse1I ACTGG 4 cut(s) 898, 1069, 1087, 2288
Bse3DI GCAATG 1 cut(s) 1098
Bse8I GATNNNNATC 1 cut(s) 1836
BseBI CCWGG 4 cut(s) 87, 459, 477, 558
BseCI ATCGAT 1 cut(s) 1364
BseDI CCNNGG 3 cut(s) 458, 1342, 2120
BseGI GGATG 6 cut(s) 385, 469, 860, 1012, 1073, 1638
BseJI GATNNNNATC 1 cut(s) 1836
BseLI CCNNNNNNNGG 4 cut(s) 777, 834, 865, 1269
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 3 cut(s) 531, 1000, 1929
BseNI ACTGG 4 cut(s) 898, 1069, 1087, 2288
BseXI GCAGC 5 cut(s) 301, 384, 750, 1177, 1396
BsgI GTGCAG 3 cut(s) 570, 1550, 2118
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 1 cut(s) 2209
BshVI ATCGAT 1 cut(s) 1364
BsiSI CCGG 1 cut(s) 1072
BslFI GGGAC 3 cut(s) 593, 881, 1383
BslI CCNNNNNNNGG 4 cut(s) 777, 834, 865, 1269
BsmAI GTCTC 3 cut(s) 338, 989, 1938
BsmFI GGGAC 3 cut(s) 593, 881, 1383
BsmI GAATGC 2 cut(s) 205, 1614
BsnI GGCC 1 cut(s) 2209
Bso31I GGTCTC 1 cut(s) 338
Bsp143I GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
Bsp1720I GCTNAGC 1 cut(s) 2126
Bsp68I TCGCGA 1 cut(s) 648
BspACI CCGC 3 cut(s) 9, 610, 945
BspANI GGCC 1 cut(s) 2209
BspCNI CTCAG 3 cut(s) 532, 999, 1930
BspDI ATCGAT 1 cut(s) 1364
BspFNI CGCG 1 cut(s) 648
BspLI GGNNCC 5 cut(s) 562, 581, 582, 869, 2118
BspMI ACCTGC 2 cut(s) 1103, 1311
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 5 cut(s) 427, 1327, 1369, 2111, 2124
BspTI CTTAAG 1 cut(s) 1312
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 1098
BsrFI RCCGGY 1 cut(s) 1071
BsrI ACTGG 4 cut(s) 898, 1069, 1087, 2288
BssAI RCCGGY 1 cut(s) 1071
BssECI CCNNGG 3 cut(s) 458, 1342, 2120
BssMI GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
BssT1I CCWWGG 1 cut(s) 2120
Bst2UI CCWGG 4 cut(s) 87, 459, 477, 558
Bst4CI ACNGT 6 cut(s) 500, 851, 955, 1031, 1879, 2235
Bst6I CTCTTC 2 cut(s) 422, 671
BstAFI CTTAAG 1 cut(s) 1312
BstAPI GCANNNNNTGC 1 cut(s) 1233
BstC8I GCNNGC 5 cut(s) 7, 943, 1700, 2054, 2252
BstDEI CTNAG 7 cut(s) 184, 540, 879, 986, 1938, 2126, 2156
BstDSI CCRYGG 1 cut(s) 1342
BstF5I GGATG 6 cut(s) 385, 469, 860, 1012, 1073, 1638
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 1 cut(s) 1191
BstHHI GCGC 2 cut(s) 1112, 1190
BstKTI GATC 7 cut(s) 422, 1322, 1364, 1801, 2119, 2149, 2330
BstMAI GTCTC 3 cut(s) 338, 989, 1938
BstMBI GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
BstMWI GCNNNNNNNGC 4 cut(s) 224, 1016, 1109, 1233
BstNI CCWGG 4 cut(s) 87, 459, 477, 558
BstNSI RCATGY 3 cut(s) 1702, 1933, 2171
BstSCI CCNGG 4 cut(s) 85, 457, 475, 556
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 5 cut(s) 301, 384, 750, 1177, 1396
BstV2I GAAGAC 2 cut(s) 720, 1972
BstX2I RGATCY 3 cut(s) 1319, 1798, 2116
BstYI RGATCY 3 cut(s) 1319, 1798, 2116
Bsu15I ATCGAT 1 cut(s) 1364
BsuRI GGCC 1 cut(s) 2209
BsuTUI ATCGAT 1 cut(s) 1364
BtgI CCRYGG 1 cut(s) 1342
BtgZI GCGATG 2 cut(s) 663, 2033
BtrI CACGTC 1 cut(s) 2297
BtsCI GGATG 6 cut(s) 385, 469, 860, 1012, 1073, 1638
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 4 cut(s) 358, 558, 843, 2231
BtuMI TCGCGA 1 cut(s) 648
BveI ACCTGC 2 cut(s) 1103, 1311
Cac8I GCNNGC 5 cut(s) 7, 943, 1700, 2054, 2252
CfoI GCGC 2 cut(s) 1112, 1190
Cfr10I RCCGGY 1 cut(s) 1071
Cfr13I GGNCC 5 cut(s) 560, 580, 868, 1300, 1460
ClaI ATCGAT 1 cut(s) 1364
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 3 cut(s) 54, 250, 891
CviQI GTAC 3 cut(s) 54, 250, 891
DdeI CTNAG 7 cut(s) 184, 540, 879, 986, 1938, 2126, 2156
DpnI GATC 7 cut(s) 421, 1321, 1363, 1800, 2118, 2148, 2329
DpnII GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
DraI TTTAAA 1 cut(s) 1549
EaeI YGGCCR 1 cut(s) 2207
Eam1104I CTCTTC 2 cut(s) 422, 671
EarI CTCTTC 2 cut(s) 422, 671
Eco130I CCWWGG 1 cut(s) 2120
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 1569
Eco47I GGWCC 5 cut(s) 560, 580, 868, 1300, 1460
Eco57I CTGAAG 2 cut(s) 1966, 2325
EcoO109I RGGNCCY 3 cut(s) 560, 580, 1300
EcoRII CCWGG 4 cut(s) 85, 457, 475, 556
EcoRV GATATC 1 cut(s) 1569
EcoT14I CCWWGG 1 cut(s) 2120
EcoT22I ATGCAT 1 cut(s) 2165
ErhI CCWWGG 1 cut(s) 2120
FaqI GGGAC 3 cut(s) 593, 881, 1383
FauNDI CATATG 1 cut(s) 1921
Fnu4HI GCNGC 7 cut(s) 315, 398, 611, 764, 946, 1191, 1410
FokI GGATG 6 cut(s) 392, 476, 867, 1019, 1080, 1645
Fsp4HI GCNGC 7 cut(s) 315, 398, 611, 764, 946, 1191, 1410
FspBI CTAG 7 cut(s) 98, 942, 981, 1442, 1706, 1809, 2105
GlaI GCGC 2 cut(s) 1111, 1189
GluI GCNGC 7 cut(s) 315, 398, 611, 764, 946, 1191, 1410
HaeII RGCGCY 1 cut(s) 1191
HaeIII GGCC 1 cut(s) 2209
HapII CCGG 1 cut(s) 1072
HhaI GCGC 2 cut(s) 1112, 1190
Hin6I GCGC 2 cut(s) 1110, 1188
HinP1I GCGC 2 cut(s) 1110, 1188
HincII GTYRAC 3 cut(s) 496, 537, 2238
HindII GTYRAC 3 cut(s) 496, 537, 2238
HindIII AAGCTT 1 cut(s) 2248
HpaI GTTAAC 2 cut(s) 496, 537
HpaII CCGG 1 cut(s) 1072
HphI GGTGA 4 cut(s) 101, 368, 436, 908
Hpy166II GTNNAC 5 cut(s) 138, 496, 537, 1342, 2238
Hpy188III TCNNGA 6 cut(s) 98, 647, 963, 1518, 1776, 1937
Hpy8I GTNNAC 5 cut(s) 138, 496, 537, 1342, 2238
HpyAV CCTTC 5 cut(s) 574, 1291, 1438, 1730, 2087
HpyCH4III ACNGT 6 cut(s) 500, 851, 955, 1031, 1879, 2235
HpyCH4IV ACGT 1 cut(s) 2296
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 1016, 1109, 1233
HpyF3I CTNAG 7 cut(s) 184, 540, 879, 986, 1938, 2126, 2156
HpySE526I ACGT 1 cut(s) 2296
HspAI GCGC 2 cut(s) 1110, 1188
KflI GGGWCCC 1 cut(s) 580
KspAI GTTAAC 2 cut(s) 496, 537
Kzo9I GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 5 cut(s) 301, 384, 750, 1177, 1396
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 7 cut(s) 98, 942, 981, 1442, 1706, 1809, 2105
MaeII ACGT 1 cut(s) 2296
MaeIII GTNAC 7 cut(s) 179, 519, 725, 920, 1025, 1811, 1925
MalI GATC 7 cut(s) 421, 1321, 1363, 1800, 2118, 2148, 2329
MboI GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 3 cut(s) 1319, 1798, 2116
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 2 cut(s) 956, 1603
MnlI CCTC 8 cut(s) 423, 672, 1441, 1503, 1514, 1623, 1688, 1945
Mph1103I ATGCAT 1 cut(s) 2165
MroXI GAANNNNTTC 3 cut(s) 970, 1721, 1960
MslI CAYNNNNRTG 4 cut(s) 213, 306, 526, 1171
MspCI CTTAAG 1 cut(s) 1312
MspI CCGG 1 cut(s) 1072
MspR9I CCNGG 4 cut(s) 87, 459, 477, 558
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 2 cut(s) 205, 1614
MvaI CCWGG 4 cut(s) 87, 459, 477, 558
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 4 cut(s) 224, 1016, 1109, 1233
NdeI CATATG 1 cut(s) 1921
NdeII GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
NheI GCTAGC 1 cut(s) 941
NlaIV GGNNCC 5 cut(s) 562, 581, 582, 869, 2118
NmuCI GTSAC 1 cut(s) 519
NruI TCGCGA 1 cut(s) 648
NsiI ATGCAT 1 cut(s) 2165
NspI RCATGY 3 cut(s) 1702, 1933, 2171
OliI CACNNNNGTG 2 cut(s) 526, 1171
PaeI GCATGC 1 cut(s) 1702
PciI ACATGT 2 cut(s) 1929, 2167
PcsI WCGNNNNNNNCGW 1 cut(s) 2336
PctI GAATGC 2 cut(s) 205, 1614
PdmI GAANNNNTTC 3 cut(s) 970, 1721, 1960
PkrI GCNGC 7 cut(s) 316, 399, 612, 765, 947, 1192, 1411
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PpuMI RGGWCCY 3 cut(s) 560, 580, 1300
PscI ACATGT 2 cut(s) 1929, 2167
PsiI TTATAA 2 cut(s) 191, 1470
Psp5II RGGWCCY 3 cut(s) 560, 580, 1300
Psp6I CCWGG 4 cut(s) 85, 457, 475, 556
PspGI CCWGG 4 cut(s) 85, 457, 475, 556
PspN4I GGNNCC 5 cut(s) 562, 581, 582, 869, 2118
PspPI GGNCC 5 cut(s) 560, 580, 868, 1300, 1460
PspPPI RGGWCCY 3 cut(s) 560, 580, 1300
PsuI RGATCY 3 cut(s) 1319, 1798, 2116
RruI TCGCGA 1 cut(s) 648
RsaI GTAC 3 cut(s) 55, 251, 892
RsaNI GTAC 3 cut(s) 54, 250, 891
RseI CAYNNNNRTG 4 cut(s) 213, 306, 526, 1171
SatI GCNGC 7 cut(s) 315, 398, 611, 764, 946, 1191, 1410
Sau3AI GATC 7 cut(s) 419, 1319, 1361, 1798, 2116, 2146, 2327
Sau96I GGNCC 5 cut(s) 560, 580, 868, 1300, 1460
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 4 cut(s) 87, 459, 477, 558
SexAI ACCWGGT 1 cut(s) 85
SinI GGWCC 5 cut(s) 560, 580, 868, 1300, 1460
SmiMI CAYNNNNRTG 4 cut(s) 213, 306, 526, 1171
SmlI CTYRAG 4 cut(s) 175, 1312, 1746, 1774
SmoI CTYRAG 4 cut(s) 175, 1312, 1746, 1774
SpeI ACTAGT 1 cut(s) 980
SphI GCATGC 1 cut(s) 1702
SsiI CCGC 3 cut(s) 9, 610, 945
SspI AATATT 1 cut(s) 667
SspMI CTAG 7 cut(s) 98, 942, 981, 1442, 1706, 1809, 2105
StyD4I CCNGG 4 cut(s) 85, 457, 475, 556
StyI CCWWGG 1 cut(s) 2120
TaaI ACNGT 6 cut(s) 500, 851, 955, 1031, 1879, 2235
TaiI ACGT 1 cut(s) 2299
TaqI TCGA 2 cut(s) 15, 1364
TatI WGTACW 1 cut(s) 53
TauI GCSGC 2 cut(s) 613, 948
TscAI CASTG 4 cut(s) 358, 558, 850, 2238
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 5 cut(s) 314, 397, 763, 1190, 1409
Tsp45I GTSAC 1 cut(s) 519
TspRI CASTG 4 cut(s) 358, 558, 850, 2238
Vha464I CTTAAG 1 cut(s) 1312
VpaK11BI GGWCC 5 cut(s) 560, 580, 868, 1300, 1460
XapI RAATTY 6 cut(s) 145, 388, 1094, 1845, 1956, 2334
XbaI TCTAGA 1 cut(s) 97
XceI RCATGY 3 cut(s) 1702, 1933, 2171
XmnI GAANNNNTTC 3 cut(s) 970, 1721, 1960
XspI CTAG 7 cut(s) 98, 942, 981, 1442, 1706, 1809, 2105
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 2165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.