FvH4_4g36180

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000223
Physical Location & Seq
Forward (+)
33228779 .. 33237206
8428 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36180.t4

Sequence Viewer

Length: 6009 bp
ATGATGGAGGGTTTTGAGTCGTCTTCTGATAGCTTCGTTGATGTTGTGTTTTCTTGGTCTCTGGAGGATATTCTCAATGAAAATTTTTACCAGCACCAGGTTGCCGGATTGGGAAAGAGTCCTCAATCATTTGGGTCGGACCTACTACAATATTTTGGTTCTTACATATATCCATTGTTAGATGAAACTCGATCTGAGTTGCAATCCAAGGTGGAAACTATCCATGGAGCACCGTTTGGTAAGGTAATATCTATTGAAGCATGCGCAAGAAATAAGCTATGTGATGTCAAAGTTGATTGTTGGAGAAACAATGTCAGTAAAGAGCCATACGAAACTAGGCCCGGTGATCTTTTCATTTTAGCTGATGCTAAACCTGAAACTGTTTCTGATCTACTAAAGGAAGGGAGATCATGGGTGTTTCTAATAGCCACTGAATTATTCCCAATAGATCAGAGCGAGGATAATACTACTTGTCTTCGTTTTAGAGTCAAAGCATCGAAAGAGTTTGATATCAACAGTGGCATTCAGACACCACTGTTTATCATTTACTTGGAGAATATAGCACCAAACATGAGAATATGGAAAGCTATGCACATGTGCACAGATGCTACTCATGCTAGATGGAAGGTGACTTTCAGTGATAACTTTCTGAAGTCGTTCAATCGACTGACGTCAATCCGAACAAAGTTGTCTGTAGTCAGTTTGCTACTTAAGCTATCAACTGGCTGGAGAGGTACGAAACGTAATGCTGAAATAGTTTGTGTTACCTCTTCGATGGTTGTGAAGAAGTTCAAAGTTCAAGGGTTATATATTCTTTGCTCAACTGACATCATAAAACATTTGAGATACATGCAAGTTTTAAAAGTATGGGACATATTGCCGGATCTTTGTGCTATCCAAACACTGGTAAATCACTTGGATCTGTTTTTCGTGAGATATACTGCCGGTTTCATCAAACTCTGCTGTGAGAAATATTTCGAGGGTGACTTGGAAGTTCCAATGACCTGGCCGCCCTCTTTGGATATTGTTCGATATAAGGATCTTAGCATGTCCCAGGATGATAGTTCTGTTGAAGGTTCAAAGTTCAATCAGAGTTTGTTACTGATGAAGTTCTACTCCTTATCATCTGGGGTGGTGAATCACTTACTCTCTGACCGTGAGGGTACAAAAATGGATCTCCCTTTTGAAGTCACAGACGAGGAAATGGACGCAATCCTTTACGGTTGGAGTACCTTTATAGTTGGACGATCAGGAACCGGTAAGACTACTGTTTTGTCAATGAAGTTGTATCAGAACGAAGAGGGTCACAATATGGCAGAAGAAGCATTGAATGGAGCAGAGGAGAGTTCTGTTCTACGACAGATTTTTGTAACAGTGAGTCCGAAACTTTGTTTTGCTGTGAAGCAACATGTTTTACATTTGAAAAGTTTTTCTTCTGGTGGAAGCCATTCATCTGAGTTCAGTTTGTTTGACATGGATGATTTTGATGCTGAGGAAGCACAGTTTGGGGATATTCCAAACTCTTTTCTTGATCTTCCGCTGAAGATCTATCCTCTTTTCATAACATTTAGTAAGTTCTTAATGATGCTGGATGGAAGTTTGGGTAATTCTTACTTTGAAAGATTCCTTGAAAAGCTAAATCTTCCTCATGGTTCACTAGAGTGTTCTAGATCTGTTCTGCTGCAAACCGTCATGAGGACAAAGGAGGTCAATTATGATAAGTTCTATTCATCATACTGGCCTCATTTTAATATCCAGATAACAAAGAAGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGTCTGGAAGCTTTGGAAGCAAGTGATGGGATATGTAGTAGGGACTGTTATGTGAAAATGTCAGGTCGTGCTTCCAACCTTAGTGAGGAGAATAAAGAGATCATATATGATATATTTCAGGTTTATGAAAAAATGAAGCTGAGGAATGGGGAGTTCGATATTGCTGATTTCGTAAATGATATTCACCGTCGGCTGAAACATGAAAATTATAGGGGTGATGAGATGGATTATGTGTACATTGATGAAGTACAGGATCTTACAATGAGTCAAGTTGCCCTATTCAAGCATATCTGTAGTAATGTCGACGAGGGTTTTGTCTTTTCGGGTGATACGGCGCAGACCATTTCAAAGGGTATTGACTTCAGATTTGAAGATATACGGCATCTGTTCTACAAGAAGTTTATGTTGGAATCGGGAAGCAACAAGCATGAAAAAGGGTTTGGCAAAGCAAAAATCTCGAACAAGTTTCATTTGACTCAGAACTTCCGCACACATAATGGTATATTGAAGTTATCACAGAGCATAATTGATCTACTGTATCATTTTTTCCCCCAATATATTGATCCTTTGAATCCTGAAACTAGTCCAGTACATGGGGAAGCTCCGGTTTTACTTGAATCCGGAGAAGATGAAAATGAAAATATGATTGGAAAGTTATTTGGGAGTAATAGTACATCAGGAAGCATTGTTGGCTTCGGAGCAGAGCAGGTTATTTTGGTGCGTGACTCTTGTGCTCGAAAGGAAATTTCCAACTTTGTTGGCAAGCAGGCTCTTGTTCTTACCATATTGGAGAGCAAGGGGCTCGAGTTTCAGGATGTGATTTTGTATGACTTTTTTGGATCTTCATCCCTCAAACAGCAATGGAGGCTGATATATGAATATATGAAGGAAAATGAATTGCTTGATCCGAGAACACCTGAGAAATTTCCAAGTTTCAATGGAGGCAAACACCATTTGCTGTGCTCTGAATTGAAGCAATTGTATGTTGCTGTTACACGCACAAAACAGAGACTTTGGATTTATGAGAAAATGGCTGATATCTGCAGTCCCATGTTTGACTTTTGGAAGAAGAAGTGTCTGATACAAGTGAGGCAAATTGATGATTCTCTTGTAGAGAAAATGAAAGTTGAAAGTACCCCAGAGGAGTGGAGAATGCGTGGCATGAAGCTATATCACCAGCATAACTATGAACTGGCCACTATGTGCTTTGAAAAAGCACATGATATTAATTGGGAAAGAAAGTCAAGAGCATCTGGCCTTAAAGTTTTGGCTGATTGTACGCAATCATCAAATCCTGAAAAGGCCAGATCCTTTCTTAGGGAAGCTGCAATAACTTTTGAAGATATAGGAATGGCAAATTCTGCTGCCAATTGCTTTTTTGATTTAGGGGATTACGAAAGGGCTGGTAGGATTTATTTGGACAAATGTGGTGAACAAGGACTGGAAAGAGCTGCAGAATGCTTTTATCTAGCAGGCAGCTATGAGGATGCGGCAAACATTTTTGAATCCATAGAAAGGCCAGAATATGCTGCTAGATGCTTTTGTAATTTGGGGATGTATGTAAGGGCAGCTACTATATATATGGAAAAATGTGGTGAACCCGGACTTCAAAAGGCCGGCGAATGCTTCTCACTAGCAGAACGCTATGATTTGGCGGCTGATGTATATGCTAGGGGGAAACTTTTCTCAGAGTGTCTTACTGCATGTTTGGTGGGAGATCTATTTGACATAGGTTTGCAGTATATTAAGGATTGGAAGCAACATGCAAGAGAAGAGTATGGTCTGGCTATAAGAGAAAAAGGGACTGATAAAACTGAACTTAAGTTTCTTGAGAGATGTGCAGTTCACTATTATGATGAGGTTGATAATATCAGACCAGAGATGAAATTGGTGAGAGCTGAACTGTTTTCCAGCCAAAAAATACTTAATTCTCATCTGTCCTCAAGCTCAAAGTATTTGTGGGAAGAAAAATCGCCTGATGATATGATAAAGTGTTTAGAAGTGAAGGAATCAAAAAAGCAGTTTTCTGTTGATTCACTGGTGTATTTCTGGAATCTTGTGAAGGACAAGATTGTTTACTTGATCAAATATTTCGATAATCTTGAAGACCAAGATGTTAACAAAAAGAGATGCTATGGAGACTTGTTTCTGAATTATTTGGGAGTATGGAGGGATTTTCATGATGATCTGAAACCAGTCTATGTTTCACTCATGTCTGATGATGATTGGGTCACAGGTAAAAATAAAAGATATATTCTGATGAAGGGGGAATTGGTCACCATAGACTATTTCCAAATTCTTTTGGATTCTCGGAATTTCTGGAGTTCCAAACTAATCTCTTGTAGTATTAAGGTCTTGAACAAGCTTGAAGAGCTCTACAAGTTCATTATCAAGAAGTCCGATTCAGTTTCTTTCCAGAGCTGGACTCTTAACCAGGTTTATGTAGCTGCAAAGATGTATGCGGTAGCGAAGTGTGTTTTGGGATCCAAGTTTTTGAATGATGAAGATCTAAACACACTAAGGAAATTTGTTGATCTGTCAGCTGAAAGTATTGTTGCTTGCATATTTCCTTTTGATTGGAGAAAAACTCTGAGAGGGAATATGATTCTTCTTAGACAAAGTGATGCTTTTAAAAAACTTCTCAAACAACTCGTTGTTGATTATACCAGCTCAACTATTGAGCTTTCCTATGGGCAAGTTGGAAGGCTAGCAATGATTATTCTGGGGTCCGGTAGTGTTGATTCTGAACTACATGAGAAGCTCGTGAAAGATTTGAAATGTAGTCGCCCATGGGAGTTGTTCATTCTGAATATGTGTGGGAGTATCATTGGAGTAAAGAAGGGGTCTGTACATGTGATTAATACTCGAGAAACAAGGTGTAACCTTGTCAGGCAGTTGCATCAAGCTTTGGTTGAAACCTATCATACAAACTGGAGGACTGTTTTTGACTATATTTCGCCTAATGGTTTCTTGTATCTTACGGAACGCCTTTTGATGTTGGTATCTTGCTTCCAAGGTTATGTTGTCACTACCAAATCTTGTTTCATTGAATGGTTGATGCAGAATAGAGATACCAAATTTACTTCTAGTACACAGGCAGATGTGCTAATATCTTTCAAACCAATCCTAAAGTTTCTGCGCCATGTGGTTCGGGAGTTTCTCTATGATAAGAAAACCACAATTCAATGGATTCAAAGGTTTATCACAGAGTGGAAGGAGTATTACTCGCTGCTGGTTCTTAGATCGGTTCTCATCTTGTGTTTGCTTTATATCAATTTTGGGATGTGTTTTGATGTACTCTTAGATGTTCTACGGAGGGAACATATCACTGAACAACTACCAACGGAGGTTTGCGATGCACTTAGGAAATTTATTTTTGCACAAGATACTCTCAGTATGGATGTGAAGGTGAATGTGCTTGCTTCAGCTTTTAAGAAGGCTGGTAATGATTTGGTAGTTGCAAGTCTTGGGATTGATTGCTCAAGGTTCTTTAGTTCAGACTCCATTTTTGTTGACATGAAGGCTATTCTGTGGTGTGATGAAACATTTTCGAAATTGTTAGCCCATTTGCATATTCATGTTCCATGTTCATCCTCTCAATATGTAGCCGTTTTTGGAGAAGCTAATGCTAATGATTGTGCTGGGCCTTCTGCTTTTGATTCGGATGAGGCTGAGATCAAAAGTTCTAAGCTTCTTCAACCCGACTCAGGTCTGGAAGCAGATGAGGTGAAAAACAGCGGGAACCTCCCTAAGGATTTTGATCACTTGTGGGATATATTGGAAGGCTTGCAGGAGGTGAAAAAAAGAGTTCATCAGAGGAGTTTATTTTCAGATGCCTCAACAAAAAAGTTGGAGTTGGAAAAATTTGTTTTCCTTTTAACTTCTGTGTGGTCTGCACGCTGTCCGAACGCATTTGAACAAATATTATCTGGAGAAGTGATGACAATGCTTGATGATTTAATGCAACTTTATGCGGCATTAGATGGGAGTGAACAAGAACTCCGGAACAAAATATCTACAGTTGCACAACTTAGCAGGAGGTTGCTCACGAGGGGGGAGAGGATTTATTGGCCTGAAAAGATAGAACCTGAATTTTCGCAGCTGTTTTTGGAGCACAATGTCAAACTTGCTGGAAAGGAACTCGAGACCATTGTCTGGGTCCGGCAACCATTTTACAGCAGAAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2003

Amino Acids

230.28

Weight (kDa)

5.84

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 442 - 531 5e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 674
Acc16I TGCGCA 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 2515
AccB7I CCANNNNNTGG 3 cut(s) 904, 2411, 5973
AccI GTMKAC 1 cut(s) 2121
AccIII TCCGGA 2 cut(s) 2438, 5820
AciI CCGC 8 cut(s) 1010, 1538, 2305, 3308, 3473, 4280, 5556, 5792
AcoI YGGCCR 2 cut(s) 1007, 3012
AcuI CTGAAG 4 cut(s) 671, 1562, 2164, 5226
AcyI GRCGYC 1 cut(s) 671
AdeI CACNNNGTG 2 cut(s) 3021, 5028
AflII CTTAAG 2 cut(s) 710, 3638
AflIII ACRYGT 3 cut(s) 594, 1408, 4669
AgeI ACCGGT 1 cut(s) 1256
AhlI ACTAGT 1 cut(s) 2399
AjnI CCWGG 4 cut(s) 96, 1004, 1053, 4251
AleI CACNNNNGTG 1 cut(s) 1660
AloI GAACNNNNNNTCC 2 cut(s) 5802, 5834
Alw21I GWGCWC 6 cut(s) 232, 602, 2554, 2783, 4194, 5934
Alw26I GTCTC 4 cut(s) 63, 2821, 3951, 5957
Alw44I GTGCAC 1 cut(s) 598
Ama87I CYCGRG 3 cut(s) 2621, 4683, 5960
Aor13HI TCCGGA 2 cut(s) 2438, 5820
ApaLI GTGCAC 1 cut(s) 598
ArsI GACNNNNNNTTYG 8 cut(s) 1255, 1287, 2114, 2146, 2168, 2200, 5309, 5341
AseI ATTAAT 2 cut(s) 3045, 4677
AsiGI ACCGGT 1 cut(s) 1256
Asp700I GAANNNNTTC 5 cut(s) 656, 788, 974, 1447, 3500
AspLEI GCGC 3 cut(s) 266, 2155, 4959
AspS9I GGNCC 5 cut(s) 139, 339, 4545, 5462, 5977
AsuC2I CCSGG 2 cut(s) 342, 3420
AsuII TTCGAA 1 cut(s) 5369
AsuNHI GCTAGC 1 cut(s) 4525
AvaI CYCGRG 3 cut(s) 2621, 4683, 5960
AvaII GGWCC 3 cut(s) 139, 4545, 5977
AxyI CCTNAGG 1 cut(s) 5568
BaeGI GKGCMC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 3014
BamHI GGATCC 1 cut(s) 4301
BanII GRGCYC 2 cut(s) 2622, 4194
BarI GAAGNNNNNNTAC 2 cut(s) 5024, 5056
BauI CACGAG 2 cut(s) 4580, 5866
BbsI GAAGAC 3 cut(s) 15, 467, 3930
Bbv12I GWGCWC 6 cut(s) 232, 602, 2554, 2783, 4194, 5934
BbvCI CCTCAGC 2 cut(s) 1491, 1958
BccI CCATC 6 cut(s) 615, 769, 1586, 1838, 2035, 5795
BceAI ACGGC 3 cut(s) 2166, 2213, 5411
BcgI CGANNNNNNTGC 4 cut(s) 2256, 2290, 2602, 2636
BciT130I CCWGG 4 cut(s) 98, 1006, 1055, 4253
BclI TGATCA 2 cut(s) 3900, 5578
BcnI CCSGG 2 cut(s) 342, 3420
BcoDI GTCTC 4 cut(s) 63, 2821, 3951, 5957
BcuI ACTAGT 1 cut(s) 2399
BfmI CTRYAG 5 cut(s) 693, 2110, 2860, 3270, 5835
BfrI CTTAAG 2 cut(s) 710, 3638
BfuAI ACCTGC 1 cut(s) 2515
BglII AGATCT 4 cut(s) 1545, 1670, 3535, 4324
Bme1390I CCNGG 6 cut(s) 98, 342, 1006, 1055, 3420, 4253
Bme18I GGWCC 3 cut(s) 139, 4545, 5977
BmeT110I CYCGRG 3 cut(s) 2621, 4683, 5960
BmgT120I GGNCC 5 cut(s) 139, 339, 4545, 5462, 5977
BmiI GGNNCC 5 cut(s) 1255, 4303, 4546, 5561, 5978
BmrFI CCNGG 6 cut(s) 98, 342, 1006, 1055, 3420, 4253
BmtI GCTAGC 1 cut(s) 4529
BoxI GACNNNNGTC 3 cut(s) 670, 5526, 5969
BpiI GAAGAC 3 cut(s) 15, 467, 3930
BplI GAGNNNNNCTC 6 cut(s) 4228, 4260, 4390, 4422, 5027, 5059
BpmI CTGGAG 5 cut(s) 83, 748, 4159, 4771, 5769
Bpu10I CCTNAGC 2 cut(s) 1491, 1958
Bpu14I TTCGAA 1 cut(s) 5369
BpuEI CTTGAG 3 cut(s) 3668, 3745, 5284
BpuMI CCSGG 2 cut(s) 342, 3420
BsaBI GATNNNNATC 3 cut(s) 2662, 4323, 5577
BsaHI GRCGYC 1 cut(s) 671
BsaI GGTCTC 2 cut(s) 63, 5957
BsaJI CCNNGG 5 cut(s) 207, 223, 1053, 4607, 4831
BsaWI WCCGGW 5 cut(s) 1256, 2422, 2438, 4547, 5820
BsaXI ACNNNNNCTCC 6 cut(s) 2473, 2503, 2959, 2989, 5802, 5832
Bse118I RCCGGY 3 cut(s) 944, 1256, 3434
Bse1I ACTGG 9 cut(s) 727, 909, 1742, 2405, 3015, 3264, 3861, 4013, 4754
Bse21I CCTNAGG 1 cut(s) 5568
Bse3DI GCAATG 2 cut(s) 2684, 4536
Bse8I GATNNNNATC 3 cut(s) 2662, 4323, 5577
BseAI TCCGGA 2 cut(s) 2438, 5820
BseBI CCWGG 4 cut(s) 98, 1006, 1055, 4253
BseDI CCNNGG 5 cut(s) 207, 223, 1053, 4607, 4831
BseJI GATNNNNATC 3 cut(s) 2662, 4323, 5577
BseMI GCAATG 2 cut(s) 2684, 4536
BseNI ACTGG 9 cut(s) 727, 909, 1742, 2405, 3015, 3264, 3861, 4013, 4754
BseRI GAGGAG 4 cut(s) 1355, 1919, 2975, 5650
BseSI GKGCMC 1 cut(s) 602
BseYI CCCAGC 1 cut(s) 5459
BsgI GTGCAG 2 cut(s) 3678, 5697
BshTI ACCGGT 1 cut(s) 1256
BsiHKAI GWGCWC 6 cut(s) 232, 602, 2554, 2783, 4194, 5934
BsiHKCI CYCGRG 3 cut(s) 2621, 4683, 5960
BslFI GGGAC 5 cut(s) 884, 1036, 1874, 2850, 3634
BsmAI GTCTC 4 cut(s) 63, 2821, 3951, 5957
BsmFI GGGAC 5 cut(s) 884, 1036, 1874, 2850, 3634
BsmI GAATGC 4 cut(s) 522, 2976, 3281, 3446
Bso31I GGTCTC 2 cut(s) 63, 5957
BsoBI CYCGRG 3 cut(s) 2621, 4683, 5960
Bsp119I TTCGAA 1 cut(s) 5369
Bsp1286I GDGCHC 7 cut(s) 232, 602, 2554, 2622, 2783, 4194, 5934
Bsp13I TCCGGA 2 cut(s) 2438, 5820
Bsp1407I TGTACA 2 cut(s) 2052, 4666
Bsp19I CCATGG 2 cut(s) 223, 4607
BspACI CCGC 8 cut(s) 1010, 1538, 2305, 3308, 3473, 4280, 5556, 5792
BspEI TCCGGA 2 cut(s) 2438, 5820
BspHI TCATGA 2 cut(s) 1692, 3997
BspLI GGNNCC 5 cut(s) 1255, 4303, 4546, 5561, 5978
BspMAI CTGCAG 2 cut(s) 2864, 3274
BspMI ACCTGC 1 cut(s) 2515
BspOI GCTAGC 1 cut(s) 4529
BspQI GCTCTTC 1 cut(s) 4182
BspT104I TTCGAA 1 cut(s) 5369
BspTI CTTAAG 2 cut(s) 710, 3638
BspTNI GGTCTC 2 cut(s) 63, 5957
BsrDI GCAATG 2 cut(s) 2684, 4536
BsrFI RCCGGY 3 cut(s) 944, 1256, 3434
BsrGI TGTACA 2 cut(s) 2052, 4666
BsrI ACTGG 9 cut(s) 727, 909, 1742, 2405, 3015, 3264, 3861, 4013, 4754
BssAI RCCGGY 3 cut(s) 944, 1256, 3434
BssECI CCNNGG 5 cut(s) 207, 223, 1053, 4607, 4831
BssNI GRCGYC 1 cut(s) 671
BssSI CACGAG 2 cut(s) 4580, 5866
BssT1I CCWWGG 4 cut(s) 207, 223, 4607, 4831
Bst2BI CACGAG 2 cut(s) 4580, 5866
Bst2UI CCWGG 4 cut(s) 98, 1006, 1055, 4253
Bst6I CTCTTC 4 cut(s) 775, 1293, 3585, 4182
BstACI GRCGYC 1 cut(s) 671
BstAFI CTTAAG 2 cut(s) 710, 3638
BstAPI GCANNNNNTGC 1 cut(s) 3179
BstAUI TGTACA 2 cut(s) 2052, 4666
BstBI TTCGAA 1 cut(s) 5369
BstDSI CCRYGG 2 cut(s) 223, 4607
BstEII GGTNACC 1 cut(s) 4093
BstENI CCTNNNNNAGG 3 cut(s) 1901, 3133, 5567
BstHHI GCGC 3 cut(s) 266, 2155, 4959
BstMAI GTCTC 4 cut(s) 63, 2821, 3951, 5957
BstMWI GCNNNNNNNGC 9 cut(s) 614, 712, 1322, 1496, 1835, 2508, 2683, 3179, 4189
BstNI CCWGG 4 cut(s) 98, 1006, 1055, 4253
BstNSI RCATGY 8 cut(s) 264, 598, 853, 1051, 1412, 3525, 3584, 4673
BstPAI GACNNNNGTC 3 cut(s) 670, 5526, 5969
BstPI GGTNACC 1 cut(s) 4093
BstSCI CCNGG 6 cut(s) 96, 340, 1004, 1053, 3418, 4251
BstSFI CTRYAG 5 cut(s) 693, 2110, 2860, 3270, 5835
BstSLI GKGCMC 1 cut(s) 602
BstV2I GAAGAC 3 cut(s) 15, 467, 3930
BstXI CCANNNNNNTGG 2 cut(s) 1005, 2964
Bsu36I CCTNAGG 1 cut(s) 5568
BtgI CCRYGG 2 cut(s) 223, 4607
BtgZI GCGATG 1 cut(s) 5187
BtsIMutI CAGTG 8 cut(s) 429, 523, 533, 643, 902, 1380, 3854, 5145
BveI ACCTGC 1 cut(s) 2515
CciI TCATGA 2 cut(s) 1692, 3997
CfoI GCGC 3 cut(s) 266, 2155, 4959
Cfr10I RCCGGY 3 cut(s) 944, 1256, 3434
Cfr13I GGNCC 5 cut(s) 139, 339, 4545, 5462, 5977
CseI GACGC 1 cut(s) 1217
CsiI ACCWGGT 2 cut(s) 96, 4251
CspAI ACCGGT 1 cut(s) 1256
DraI TTTAAA 2 cut(s) 861, 4450
DraIII CACNNNGTG 2 cut(s) 3021, 5028
EaeI YGGCCR 2 cut(s) 1007, 3012
Eam1104I CTCTTC 4 cut(s) 775, 1293, 3585, 4182
EarI CTCTTC 4 cut(s) 775, 1293, 3585, 4182
Ecl136II GAGCTC 1 cut(s) 4192
Eco130I CCWWGG 4 cut(s) 207, 223, 4607, 4831
Eco24I GRGCYC 2 cut(s) 2622, 4194
Eco31I GGTCTC 2 cut(s) 63, 5957
Eco32I GATATC 2 cut(s) 511, 2857
Eco47I GGWCC 3 cut(s) 139, 4545, 5977
Eco53kI GAGCTC 1 cut(s) 4192
Eco57I CTGAAG 4 cut(s) 671, 1562, 2164, 5226
Eco81I CCTNAGG 1 cut(s) 5568
Eco88I CYCGRG 3 cut(s) 2621, 4683, 5960
Eco91I GGTNACC 1 cut(s) 4093
EcoICRI GAGCTC 1 cut(s) 4192
EcoNI CCTNNNNNAGG 3 cut(s) 1901, 3133, 5567
EcoO65I GGTNACC 1 cut(s) 4093
EcoRII CCWGG 4 cut(s) 96, 1004, 1053, 4251
EcoRV GATATC 2 cut(s) 511, 2857
EcoT14I CCWWGG 4 cut(s) 207, 223, 4607, 4831
EcoT38I GRGCYC 2 cut(s) 2622, 4194
ErhI CCWWGG 4 cut(s) 207, 223, 4607, 4831
FalI AAGNNNNNCTT 4 cut(s) 1417, 1449, 4429, 4461
FaqI GGGAC 5 cut(s) 884, 1036, 1874, 2850, 3634
FauI CCCGC 1 cut(s) 5549
FbaI TGATCA 2 cut(s) 3900, 5578
FblI GTMKAC 1 cut(s) 2121
FriOI GRGCYC 2 cut(s) 2622, 4194
FspI TGCGCA 1 cut(s) 265
GlaI GCGC 3 cut(s) 265, 2154, 4958
GsaI CCCAGC 1 cut(s) 5463
GsuI CTGGAG 5 cut(s) 83, 748, 4159, 4771, 5769
HgaI GACGC 1 cut(s) 1217
HhaI GCGC 3 cut(s) 266, 2155, 4959
Hin1I GRCGYC 1 cut(s) 671
Hin6I GCGC 3 cut(s) 264, 2153, 4957
HinP1I GCGC 3 cut(s) 264, 2153, 4957
HincII GTYRAC 3 cut(s) 2122, 3937, 5332
HindII GTYRAC 3 cut(s) 2122, 3937, 5332
HindIII AAGCTT 5 cut(s) 1769, 1827, 4181, 4722, 5507
HpaI GTTAAC 1 cut(s) 3937
Hpy99I CGWCG 2 cut(s) 2010, 2126
HpyCH4IV ACGT 2 cut(s) 671, 742
HpyF10VI GCNNNNNNNGC 9 cut(s) 614, 712, 1322, 1496, 1835, 2508, 2683, 3179, 4189
HpySE526I ACGT 2 cut(s) 671, 742
Hsp92I GRCGYC 1 cut(s) 671
HspAI GCGC 3 cut(s) 264, 2153, 4957
Kpn2I TCCGGA 2 cut(s) 2438, 5820
KroI GCCGGC 1 cut(s) 3434
KroNI GCCGGC 1 cut(s) 3436
Ksp22I TGATCA 2 cut(s) 3900, 5578
KspAI GTTAAC 1 cut(s) 3937
LguI GCTCTTC 1 cut(s) 4182
LmnI GCTCC 5 cut(s) 227, 1334, 2425, 2516, 5929
MabI ACCWGGT 2 cut(s) 96, 4251
MaeII ACGT 2 cut(s) 671, 742
MfeI CAATTG 2 cut(s) 2795, 3187
MhlI GDGCHC 7 cut(s) 232, 602, 2554, 2622, 2783, 4194, 5934
MlsI TGGCCA 1 cut(s) 3014
MluNI TGGCCA 1 cut(s) 3014
Mox20I TGGCCA 1 cut(s) 3014
MroI TCCGGA 2 cut(s) 2438, 5820
MroNI GCCGGC 1 cut(s) 3434
MroXI GAANNNNTTC 5 cut(s) 656, 788, 974, 1447, 3500
MscI TGGCCA 1 cut(s) 3014
MslI CAYNNNNRTG 4 cut(s) 1660, 3003, 3669, 5394
Msp20I TGGCCA 1 cut(s) 3014
MspA1I CMGCKG 4 cut(s) 1540, 4361, 5556, 5920
MspCI CTTAAG 2 cut(s) 710, 3638
MspR9I CCNGG 6 cut(s) 98, 342, 1006, 1055, 3420, 4253
MunI CAATTG 2 cut(s) 2795, 3187
Mva1269I GAATGC 4 cut(s) 522, 2976, 3281, 3446
MvaI CCWGG 4 cut(s) 98, 1006, 1055, 4253
MwoI GCNNNNNNNGC 9 cut(s) 614, 712, 1322, 1496, 1835, 2508, 2683, 3179, 4189
NaeI GCCGGC 1 cut(s) 3436
NciI CCSGG 2 cut(s) 342, 3420
NcoI CCATGG 2 cut(s) 223, 4607
NgoMIV GCCGGC 1 cut(s) 3434
NheI GCTAGC 1 cut(s) 4525
NlaIV GGNNCC 5 cut(s) 1255, 4303, 4546, 5561, 5978
NmuCI GTSAC 8 cut(s) 628, 983, 1189, 1304, 2540, 4048, 4093, 4843
NsbI TGCGCA 1 cut(s) 265
NspI RCATGY 8 cut(s) 264, 598, 853, 1051, 1412, 3525, 3584, 4673
NspV TTCGAA 1 cut(s) 5369
OliI CACNNNNGTG 1 cut(s) 1660
PaeI GCATGC 1 cut(s) 264
PaeR7I CTCGAG 3 cut(s) 2621, 4683, 5960
PagI TCATGA 2 cut(s) 1692, 3997
PciI ACATGT 3 cut(s) 594, 1408, 4669
PciSI GCTCTTC 1 cut(s) 4182
PctI GAATGC 4 cut(s) 522, 2976, 3281, 3446
PdiI GCCGGC 1 cut(s) 3436
PdmI GAANNNNTTC 5 cut(s) 656, 788, 974, 1447, 3500
PflMI CCANNNNNTGG 3 cut(s) 904, 2411, 5973
PinAI ACCGGT 1 cut(s) 1256
PscI ACATGT 3 cut(s) 594, 1408, 4669
PshAI GACNNNNGTC 3 cut(s) 670, 5526, 5969
PshBI ATTAAT 2 cut(s) 3045, 4677
Psp124BI GAGCTC 1 cut(s) 4194
Psp6I CCWGG 4 cut(s) 96, 1004, 1053, 4251
PspEI GGTNACC 1 cut(s) 4093
PspFI CCCAGC 1 cut(s) 5459
PspGI CCWGG 4 cut(s) 96, 1004, 1053, 4251
PspN4I GGNNCC 5 cut(s) 1255, 4303, 4546, 5561, 5978
PspPI GGNCC 5 cut(s) 139, 339, 4545, 5462, 5977
PspXI VCTCGAGB 1 cut(s) 2621
PstI CTGCAG 2 cut(s) 2864, 3274
PvuII CAGCTG 2 cut(s) 4361, 5920
RseI CAYNNNNRTG 4 cut(s) 1660, 3003, 3669, 5394
SacI GAGCTC 1 cut(s) 4194
SalI GTCGAC 1 cut(s) 2120
SapI GCTCTTC 1 cut(s) 4182
Sau96I GGNCC 5 cut(s) 139, 339, 4545, 5462, 5977
ScrFI CCNGG 6 cut(s) 98, 342, 1006, 1055, 3420, 4253
SduI GDGCHC 7 cut(s) 232, 602, 2554, 2622, 2783, 4194, 5934
SexAI ACCWGGT 2 cut(s) 96, 4251
SfcI CTRYAG 5 cut(s) 693, 2110, 2860, 3270, 5835
Sfr274I CTCGAG 3 cut(s) 2621, 4683, 5960
SfuI TTCGAA 1 cut(s) 5369
SinI GGWCC 3 cut(s) 139, 4545, 5977
SlaI CTCGAG 3 cut(s) 2621, 4683, 5960
SmiMI CAYNNNNRTG 4 cut(s) 1660, 3003, 3669, 5394
SmlI CTYRAG 8 cut(s) 710, 2621, 3638, 3647, 3760, 4683, 5299, 5960
SmoI CTYRAG 8 cut(s) 710, 2621, 3638, 3647, 3760, 4683, 5299, 5960
SpeI ACTAGT 1 cut(s) 2399
SphI GCATGC 1 cut(s) 264
SsiI CCGC 8 cut(s) 1010, 1538, 2305, 3308, 3473, 4280, 5556, 5792
SspI AATATT 4 cut(s) 152, 974, 3908, 5742
SstI GAGCTC 1 cut(s) 4194
StyD4I CCNGG 6 cut(s) 96, 340, 1004, 1053, 3418, 4251
StyI CCWWGG 4 cut(s) 207, 223, 4607, 4831
TaiI ACGT 2 cut(s) 674, 745
TatI WGTACW 7 cut(s) 2052, 2065, 2407, 2489, 4666, 4907, 5113
TauI GCSGC 4 cut(s) 1012, 3311, 3476, 5795
TscAI CASTG 8 cut(s) 436, 523, 540, 643, 909, 1380, 3861, 5152
TseFI GTSAC 8 cut(s) 628, 983, 1189, 1304, 2540, 4048, 4093, 4843
Tsp45I GTSAC 8 cut(s) 628, 983, 1189, 1304, 2540, 4048, 4093, 4843
TspGWI ACGGA 3 cut(s) 4814, 5146, 5177
TspRI CASTG 8 cut(s) 436, 523, 540, 643, 909, 1380, 3861, 5152
Van91I CCANNNNNTGG 3 cut(s) 904, 2411, 5973
Vha464I CTTAAG 2 cut(s) 710, 3638
VneI GTGCAC 1 cut(s) 598
VpaK11BI GGWCC 3 cut(s) 139, 4545, 5977
VspI ATTAAT 2 cut(s) 3045, 4677
XagI CCTNNNNNAGG 3 cut(s) 1901, 3133, 5567
XbaI TCTAGA 1 cut(s) 1667
XceI RCATGY 8 cut(s) 264, 598, 853, 1051, 1412, 3525, 3584, 4673
XhoI CTCGAG 3 cut(s) 2621, 4683, 5960
XmiI GTMKAC 1 cut(s) 2121
XmnI GAANNNNTTC 5 cut(s) 656, 788, 974, 1447, 3500
ZraI GACGTC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.