Rroxscaffold_6G00396020

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000223
Physical Location & Seq
Reverse (-)
17253193 .. 17264376
11184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396020.1

Sequence Viewer

Length: 7965 bp
ATGCTGGGTTTAACTGATACAGTGTTTTCTTGGTCCCTTGAAGATATTTTCAATGAAGATCTTTACAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCAGTCGGTTGTCCAGTACTTTGGGTCTTATCTGTATCCTTTATTGGAAGAAACTCGAGCTCAGTTACATTCCAGTATGGAAACTATTGACAGTGCACCATTTGCGGAAGTAGTTGATTTTAAAGAGTCCAAGCCATATGGAACAAAGCAATATACAGTCAAGGTTGATTGTTGGCGAAACAGGTTCAGTGAACGTGGCAATGAACCATACAAAACTTTGCCTGGGGATCTTTTTATTTTAGCAAATGCTAAACCTGAAACTGTTTCTGAATTACAACTGGCTGGGAGGTCATGGTCTTTTGTATCTGTCACTCATCTTGTGTCTAACATCTCACAAATTCAGAAAAAAGATGAGAATGGGGATGAGGTTGAGGATGTGGAAGAGGAAACTTCTCTTTGTTTTAAAATCAAGTCTTCAAAAGAGTTTGAAGTCGATGATGGCACATCACTGTCTGTTGTTTTCTTGTTGAATTTAATCCCCAGTGAAAGAATATACAAAGCATTGCACATGCTGGGAAATTTAACAATTATCAAAGAAGTTCTATGCTCTGATTCTGTGGCTCAGGAAAATCTTTCCTATGAGAAGAATAATGACATCATGGAGAAGGGGTTAAATGAAAATTTATTTCCAGGACTTAATGAGGCCCAAACTGGGGCAGTTCTGGCTTGTCTTGGAATGCAGCATCGTGGTGCAAAGTCTTCGGTGGAACTTATATGGGGTCCTCCTGGGACAGGAAAAACTAAAACAATCGCTACTCTGCTTTATACTCTGTTAAAGATGAATTGCAGGACTCTTATTTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGTCTTCTGAAAATGGTAACAGAAGCAGAGTCCAATGCAATGTTTTGTTCAGTTGGAAAAATCCTTTTATTTGGGAATAAGGAACGACTCAAACTCAAAGATGGTTCAGATATTGAAGAGATATATTTGGGTCACCGTATTCAAAAGCTCATCGAGTGCATAGGACCACAAACTGGTTGGAGGCATTGCTTTGCTTCCATGATAAATTTACTTAAAGATTGTGTTCGTGATTACAATATTTACTTGTGCAATGAGTTGACTGAAGGGGAAGAACAAAATAGTGCTTGTGAAGTGAATGATGAATGCAGAAGTGGACCAGGAGTTAGCGAGGTGAAGTGCAAAACATTTCTTGAATATTTCAGAAATAGATTTCTCTCTACCGCACTGCCACTAAGATATTGCATTTCTATCTTCTGTACCCATATGACCAAAAGCTGCATTCCAGAACACATATTCAGGGATATGGTTTCTCTTATCCTCTTAGTTGATTCCTTTGAGATGTTGTTGTTTGAAGGTAATGTTATTTCAGAAGCATTGGAAGACCTTTTTTCTTGTTCAGAGGTTGAGGGTGGATCTGAGTTACATGTAGATGACTCAATTCCTCTTGTCATGAAGAGAAAGGAATGCCTTATAGCTTTACGCACTCTACAGTATTCTCTTAGTAAACTTCAACTTCCAGATTTTAGGAGAGGAACTGATCTAATGGATTTATGTTTCCGAGGTGCATCCTTAGTATTTTGCACTGCATCTAGTTCATTTAAGCTGCATAGACTGGAAATGGAGCCATTTACCTTTGTGGTTATTGATGAAGCTGCACAGTTGAAAGAGTGCGAGTCAACTATACCCCTGCAACTACGAGGAGTAAAACATGTAATTCTTGTTGGTGATGAATGCCAATTGCCAGCTATGGTGAAAAGCAATGTTTCTCAAGAAGCAGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCACAATGGGTCACCCAAAACACCTTCTAAATATGCAGTACAGAATGCATCCGTCGATAAGTTTCTTCCCAAATTCTAGATTCTATAATAACCTGATCCTGGATGCTCCAAATGTCACAAAAAAAAGCCATGAGGAGCACTATCTTCCAGGGCCAATATATGGTCCTTATTCTTTTATAAATGTAAATGATGGAAGAGAGGAGAAGGATGAGGATGGACATAGTCGAAAGAATATGGTTGAGGTCGCTATTGTTTTGAAAGTACTGCGGAATCTTTACAAAGAATGGATGAATTCAAACCAGAATCTCAGTGTTGGAGTGGTATCTCCCTATGCTGCTCAAGTAGTGGCAATTCAGGAAAATCTTAGACAAAAGTATGACAATCTTGATGGCTTTAAAGTGAAGGTAAATACAGTTGACGGGTTCCAGGGTGGGGAAGACGACATAATTATAATGTCCACTGTAAGATCCAACAGCCATCAATCACTTCAGTTTATTTCAAATATTCAAAGAGTCAATGTTGCTCTCACAAGGGCTAGGCACTGTCTCTGGATTTTGGGCAATGAAAAAACTCTATGCAGTAGTGATTCTGTTTGGAAGGACTTGGTTCTTGATGCCAAAAGTCGTGAACGCTTCTTTAATGCTGATCAAGACAAGGATTTGGCCAAGGCCATAGTAGACGTAAAGAAAGAGTTGGACCAATTGGATGATTTGCTCAACGCCGACAGCATACTTTTCAAAAATGCCAGATGGAAGGTGCTTTTTAGTGATAACTTTTTGAAGTCATTCAAGAAACTGACGTCAGCCTCTTTGAAGAAGTCAGTACTAAACCTTCTACTGAAGCTTTCAACTGGCTGGAGACCTAAGAAGAGAAATGTAGAGACAAGTTGTAGTAGCTTTTCACTAATCTTGAAGAAATTTAAGGTTAAATCTCTGTATATTGTTTGCACGACAGACATTGCAAAGGATATGAGATACATTCAAATGTTAAAGGTCTGGGACATATTGCCCCTGGAGGATATCCCAAACTTGGAATATCGCCTGGAGACTGTTCTAAACAGATATACAGATGATTTTATTAATCTTTGCAAGGAGAAATATCTTGATGGTGATTTAGAAGTTCCACAGAGCTGGCCACTTTCTCTGGAATTTTCCCAGTTTAAGGATCTTAGTGTCACTGAAACCAAGAGAGATTTTGTTGGTGACACTTCTGATGGCGGAAGTTATGTTGAGAACTCACAGGTCAGCGAGAGTTTGTTGCTAATGAAATTTTACTCTTTATCATCTGGTTTGGTGAACCACTTGCTCTCTGGCCGTGAGGGTAGAGAATTGGATCTTCCGTTTGAAGTTACAGACCAAGAAATGGAGATTATACTTCATCATAGAAGTTCTTTTATAGTTGGACGGTCAGGTACTGGGAAGACCACCGTCTTAACCATGAAGTTATTCCAAAGAGAACAATGGCACCATTTGGCTGTGCAGGGATGTTATGGTACTCAAAATAACAATGATGAGCAAAGTTCTGCAACTACTGAAGGGAAAATTCTACGCCAGCTTTTCGTTACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCAACATGTTACACGATTGAAAAGTTTTGCATGTGGTGGAAGTGACTCAGCTGAAAGAAGTTTGACTGATACAGTTGATTTTGATGAGGAGGAATCCCAATTCAAGGATATCCAAGATTCATTTCATGATATTCCTCCGAGTTCTTACCCTCTTGTGATAACATTCTATAAGTTCCTGATGATGCTTGATGGAACATTGAGTAACTCATACTTTGCAAGGTTCTGCGATGCGTCACTTCCTCATGGCCAATTTAGAAATTCGAGATCAGTTGTGTTCCAGGCCTTTATAAGGACAAAAGAAGTCAATTATGAGAGGTTTAGTTCATCATATTGGCCCCATTTTAATACTCAGCTAACTAAGAACCTAGATGTTGCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGCTTGGGAACTATAGAAGCATGTGATGGGAAGCTCAGCCGGGCGGATTATGTTAAATTGTCAGAGGGCCGAGCTTCTAATTTAAGCAAGCAAAAGAGAGAACAGATATATGAGATATTTCAAGCTTATGAAAAAATGAAGATGCGAAATGGTGAATTTGATCTGGCTGATTTTGTAAATGATCTTCAACGTCGACTCAAGCATGAGAAATATATGGGTGATCAGATGGATTTTATTTACATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTCAAACATATGTGCAACAATGTTGATGAGGGATTTGTTTTTTCTGGTGATACAGCACAAACAATTGCCAGGGGTATTGATTTTAGGTTCCAAGATATAAGACATCTGTTCTACAAGAAGTTTGTGTTGGAGTCAAGAAGCAATAAGCAAGATGAAAGAAAAGGAAAAGGCCAAATTTCTGAAATATATCATTTGACTCAAAACTTCCGTACCCATTCTGGTATATTGAAGTTATCGCAGAGCATTATTGAATTACTATATCGCTTTTTTCCTCTTTCAATTGATGTTTTAAAACCAGAAAGCAGTCTGATTTATGGGGAAGCTCCGGTTGTGCTTGAATCTGGAGAAAATGAAAATGCAATCATCAAAATATTTGGAAATAGTAGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATATTGGTGCGAGATGATAGTACTCGAAGAGAAATTTCCAATGTTGTTGGGAAGCATGCTCTTGTCCTTACCATAGTTGAGTGCAAGGGCCTTGAGTTTCAGGATGTACTCTTGTACAACTTTTTCGGCTCATCTCCTTCGAAAAATCAATGGAGGGTTATTTATGATTACATGAAGGAACAAGATTTACTCGATTCTACATTGCCTAAGAAATTTCCAAGTTTCAGTGAAGCGAAACACAACATATTATGCTCTGAATTGAAGCAATTATATGTTGCTGTTACTCGCACCAGACAGAGATTGTGGATTTGTGAAACTGTAGAGGAGATTTCGAAACCCATGTTTGACTATTGGAAGAAGAAATGTCTTGTGCAAGTTAGGCAACTTGATGATTCACTTGCACAAGCCATGCAAGTTGCAAGCAGCACAGAGGAGTGGAGATCCCGTGGGATTAAGCTATATCGTGAGAATAACTACGAGATGGCCACAATGTGCTTTAAAAGATCTGGTGACACTTATTGGGAACAAAGGTCCAAAGCTGCTGGCCTTAAAGCTATTGCTGACCGTATGCGAATGTCAAATCCTGAAGAGGCTAATTCTTTTCTTAGGAAAGCTGCTGAAATATTTGATGCTATAGGGAAGGCTGATTCTGCTGCAAGATGTTTTTCTGATTTGGGAGAGTATGAAAGAGCAGCCAGGATTTATTTTGAGAAATGTGGCGAATTGGAAAGAGCTGCTGAATGTTTCACTCTAGCAGGATGCTATGAGGATGCTGCAGATGTGTATGCTAAAGGCAATTGTTTCTTAGAGTGTCTCTCTGTCTGTGCCAAAGGAAAATTATTTGATAGAGGTTTGGAATACATCAATTATTGGAAGAGGCATGCAGTAGAGAACAATTCTTTAACTAGAGGAGGAGAGGGGATACATAAGATGGAACAAAAATTTCTAGAGGGCTGTGCACGCCACTATCATAAGATGAAAGATAACAGATCCATGATGAAATTTGTAAAAGCCTTCCAGTCTATATCATTGATACGGAATTTTTTGAAGAGTTTAGGTAGCCTTGATGAACTTCTTTTGCTGGAAGATCAATTTGGAAACTATTTGGAAGCTGCGGAGATTGCACAGATTAAAGGCGAAATTCTACTTAAATCGGATTATCTTGGAAAGGCAGGTGAATTCAAAGATGCCTCAATGCATGTCCTATTCTATGTACTTGCCAGCTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCCATGAAGAAGTTTGCACAAAAGGAAGCTCTTTTGACAGAAGCAAAGTCAGTTGCCAAGAATGAGACTGACGACTTTTATGAGTTGGTTTGCACTGAGGTTGATATTTTATTGAATGGGCAGGATAACTTGGCTGTGTTGAAACATCAAATGAAGGCTTCTCAGAGACACGCAAGTATTATAGGTGAAGTGTTATCTGCTCAAAAAATTTTGGATTCCCATTTTGCTCTAAGGACTGATAAGTATTTGTGGGAAAAAGAATTGATCAGTGATTTTGTGAAGCATTCTGAAGACATGATATCCCAAAATCTGGTTTCCATCGAATCACTAGTTTACTTCTGGAATTTTTGGAAGGACAAGATTGTTCTCGTGATTGAATGTCTAGGATGCCTTGAAACCCAAGATGTTAATGTAAACAGAAGTTATCAAAAGTTTTGTTTAAACTACTTGGGAGTATGGAGGTTATCTAATCAAATGAATCCTGTGTACGTTTTACTTTTCCCTGATGCTGATTGGGTTAGAGATGTGGACAAAAGTTATTTCCGAAGTCATGGGAAGTTGGTCTCCATTGATGTTCACCAGCTTATTTCAGCTGCTCAGAAATATTGGTGTTCAGAACTGCTTGCTGTAGGCATGAAGGTTTTGGAAAAGCTTGATGAGCTTTTCAAGTTCTCTATCAAGAGTTCTGATTCAACATTCTTCCAGAGCAGGGCTCTTACCTTCATCTATGATGCTGCGGTATATCTCTTGAATTCCAAATGTTTGAAGAAAAGGCCTCAAGATGCCAAGGCACTGCAGAAATTTGTAACTTTCTCAACTGAAAGGTTTGTTGCTTGCGTGTTTCCTTTAGATTGGAGGAAATCTTTTAGAGAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATACATCAGCTCAAAGAACGAGCTGTCTTATGGTCAAATTGGAAGGATCGCAATGACCATTCTCGGGTCTGGTAAGCTGAATGTTGAACTATGTAAGAAGCTTGTGAAAATGTTGGACTCCAATCCACCATGGAAGGACTTTTTTGAGAACCTATGTGCGAGTACTGAACCTGGAAATACCAGTCAGAAGCCTAGAGAGGTGTCTTTTATGTTGCTATTATCTGAGGCTTTAGTTGAAACTTATAATGCAAACTGGAGGATTGTTAAAGACTACATATCACCTGGTTGCTTCTTGTATCTTATGGAGCGTCTTCTGATTTGGGCTTCTAAATGTCGAGGTTATGTTATCACCACAAGTTCTTGTTTCACTGAATGGCTGATGTACCAGGATGAAGATACTCATGTGCAACCATCTATAGAAAGCATCCTTCATCTTGTTAGTAATGTGGTTACCGAATGCCTTTGTAACAGGGAAAATCTGATTGAATGGATTAAAAAGTCTAATGCAGATTGGAAGACCTGTTATTCACTACTCGTGACAAGATTGGTGTTTGTACTATGTTTGATTTGTGTGAACTTTGGAATATCCTTGGGTCTACTGTCTGATATGCTGGGAAGCAGCTATATCACTGAACAACTTCCATCGGAATTTTACGACACCCTTAAGAGGCGGAGGAGGAACAATTTTCTGAATATAAATGAGGAAGTGCTTGCAGCAGCTCTCAAGAAGATTCATAATCCTTTGGTAATTGCGAGTTTTGGGTTTGATTGTTCAAGGTTCGTATGCCCAGATGCCATCTTTGTTGACATGAAGGCCATTCAGTCCAAGGATGACCTATTCAGAATAATGTTTCCTAAACCACCAGTCATTCAAGTTGCACCAGGTCGAATTGGATTTGTTGAAGCTGGAGGTAATACTCTTTGTGATGGTGTACCTTCTGCAAATGATATCGATGCAGGGAAGAGCTCTTTAAACTCCAACTTGGTGGGAGAAACTGATGAGCAGCTTGATGCTGAAGACGGTCAGAGTAGAGGTGAGGAAATCTGCAAAGCCGGTGTGTCTACACCAATGTGTCCACATCCTGATGCAGTCTCGGCTTCTACTTCTGCAACCCAAGCAAACAAAAAGGGCAACCGCAAGAATAATACTAAGAAAAATAAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2654

Amino Acids

302.51

Weight (kDa)

6.16

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 66 - 207 4.8e-48 Domain of unknown function (DUF6469)
AAA_11 PF13086 245 - 619 1.4e-33 AAA domain
AAA_19 PF13245 264 - 615 8.9e-09 AAA domain
AAA_12 PF13087 627 - 823 3.6e-57 AAA domain
UvrD-helicase PF00580 1330 - 1419 2.3e-08 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 2081, 2354, 3785, 7041
AarI CACCTGC 2 cut(s) 4594, 5771
AatII GACGTC 1 cut(s) 2705
Acc36I ACCTGC 3 cut(s) 1862, 4594, 5771
AccB1I GGYRCC 1 cut(s) 3366
AccB7I CCANNNNNTGG 4 cut(s) 1109, 2063, 3265, 6172
AccI GTMKAC 4 cut(s) 2580, 4102, 7393, 7859
AciI CCGC 9 cut(s) 203, 1317, 2170, 3120, 3953, 5723, 6599, 7468, 7933
AclWI GGATC 9 cut(s) 333, 1516, 1993, 2364, 3075, 3243, 5043, 5592, 6851
AcoI YGGCCR 5 cut(s) 2565, 3035, 3214, 3742, 5091
AcuI CTGAAG 8 cut(s) 945, 1218, 2375, 2762, 3456, 5214, 6171, 7833
AcyI GRCGYC 1 cut(s) 2702
AdeI CACNNNGTG 1 cut(s) 5100
AflII CTTAAG 1 cut(s) 7460
AflIII ACRYGT 3 cut(s) 1519, 1804, 3502
AhlI ACTAGT 1 cut(s) 6190
AjuI GAANNNNNNNTTGG 6 cut(s) 3603, 3635, 4301, 4333, 5685, 5717
AleI CACNNNNGTG 1 cut(s) 7867
Alw21I GWGCWC 5 cut(s) 160, 196, 2043, 5569, 7766
Alw26I GTCTC 9 cut(s) 2453, 2755, 2777, 2942, 5426, 5921, 6022, 6430, 7894
Alw44I GTGCAC 2 cut(s) 192, 5565
AlwI GGATC 9 cut(s) 333, 1516, 1993, 2364, 3075, 3243, 5043, 5592, 6851
AlwNI CAGNNNCTG 1 cut(s) 3317
Ama87I CYCGRG 2 cut(s) 153, 6860
ApaLI GTGCAC 2 cut(s) 192, 5565
AseI ATTAAT 1 cut(s) 2982
Asp700I GAANNNNTTC 5 cut(s) 84, 96, 2687, 3347, 7434
AsuC2I CCSGG 1 cut(s) 3950
AsuII TTCGAA 2 cut(s) 4748, 4940
AvaI CYCGRG 2 cut(s) 153, 6860
AvaII GGWCC 7 cut(s) 33, 818, 1100, 1250, 2066, 2599, 5139
BaeGI GKGCMC 3 cut(s) 196, 904, 5569
BaeI ACNNNNGTAYC 4 cut(s) 4384, 4417, 7185, 7218
BalI TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
BanI GGYRCC 1 cut(s) 3366
BanII GRGCYC 3 cut(s) 160, 6577, 7766
BarI GAAGNNNNNNTAC 4 cut(s) 2718, 2750, 4077, 4109
BauI CACGAG 2 cut(s) 6230, 7331
Bbv12I GWGCWC 5 cut(s) 160, 196, 2043, 5569, 7766
BceAI ACGGC 1 cut(s) 3201
BcgI CGANNNNNNTGC 2 cut(s) 780, 814
BciVI GTATCC 2 cut(s) 144, 5523
BclI TGATCA 3 cut(s) 2548, 4129, 6126
BcnI CCSGG 1 cut(s) 3950
BcoDI GTCTC 9 cut(s) 2453, 2755, 2777, 2942, 5426, 5921, 6022, 6430, 7894
BcuI ACTAGT 1 cut(s) 6190
BfmI CTRYAG 8 cut(s) 1583, 3921, 4926, 5241, 5382, 6489, 6656, 7212
BfrI CTTAAG 1 cut(s) 7460
BfuAI ACCTGC 3 cut(s) 1862, 4594, 5771
BfuI GTATCC 2 cut(s) 144, 5523
BglII AGATCT 3 cut(s) 58, 4165, 5111
BlpI GCTNAGC 1 cut(s) 3944
BmcAI AGTACT 5 cut(s) 116, 2166, 2727, 4630, 6961
Bme18I GGWCC 7 cut(s) 33, 818, 1100, 1250, 2066, 2599, 5139
BmeT110I CYCGRG 2 cut(s) 153, 6860
BmiI GGNNCC 7 cut(s) 35, 819, 1719, 2327, 3368, 3833, 4280
BmrI ACTGGG 4 cut(s) 573, 759, 3052, 3327
BmuI ACTGGG 4 cut(s) 573, 759, 3052, 3327
BoxI GACNNNNGTC 1 cut(s) 3329
BplI GAGNNNNNCTC 4 cut(s) 5408, 5440, 6559, 6591
BpmI CTGGAG 6 cut(s) 2779, 2936, 2966, 4554, 7072, 7725
Bpu10I CCTNAGC 2 cut(s) 660, 3477
Bpu1102I GCTNAGC 1 cut(s) 3944
Bpu14I TTCGAA 2 cut(s) 4748, 4940
BpuEI CTTGAG 6 cut(s) 1848, 2226, 4091, 4721, 6624, 7505
BpuMI CCSGG 1 cut(s) 3950
Bsa29I ATCGAT 1 cut(s) 7749
BsaHI GRCGYC 1 cut(s) 2702
BsaI GGTCTC 2 cut(s) 2755, 6430
BsaWI WCCGGW 1 cut(s) 4516
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 5038, 5068
Bse118I RCCGGY 1 cut(s) 7850
BseCI ATCGAT 1 cut(s) 7749
BseRI GAGGAG 9 cut(s) 1809, 2051, 2117, 3599, 4946, 5054, 5532, 5535, 7486
BseSI GKGCMC 3 cut(s) 196, 904, 5569
BseYI CCCAGC 4 cut(s) 4, 380, 610, 7408
BsgI GTGCAG 3 cut(s) 1734, 3401, 4617
BshNI GGYRCC 1 cut(s) 3366
BshVI ATCGAT 1 cut(s) 7749
BsiHKAI GWGCWC 5 cut(s) 160, 196, 2043, 5569, 7766
BsiHKCI CYCGRG 2 cut(s) 153, 6860
BsiSI CCGG 3 cut(s) 3949, 4517, 7851
BslFI GGGAC 3 cut(s) 19, 841, 2915
BsmAI GTCTC 9 cut(s) 2453, 2755, 2777, 2942, 5426, 5921, 6022, 6430, 7894
BsmFI GGGAC 3 cut(s) 19, 841, 2915
BsmI GAATGC 8 cut(s) 780, 1244, 1374, 1565, 1832, 1953, 6145, 7259
Bso31I GGTCTC 2 cut(s) 2755, 6430
BsoBI CYCGRG 2 cut(s) 153, 6860
Bsp119I TTCGAA 2 cut(s) 4748, 4940
Bsp1286I GDGCHC 7 cut(s) 160, 196, 904, 2043, 5569, 6577, 7766
Bsp1407I TGTACA 1 cut(s) 4722
Bsp1720I GCTNAGC 1 cut(s) 3944
Bsp19I CCATGG 1 cut(s) 6926
BspACI CCGC 9 cut(s) 203, 1317, 2170, 3120, 3953, 5723, 6599, 7468, 7933
BspDI ATCGAT 1 cut(s) 7749
BspHI TCATGA 2 cut(s) 1545, 3622
BspLI GGNNCC 7 cut(s) 35, 819, 1719, 2327, 3368, 3833, 4280
BspMAI CTGCAG 2 cut(s) 5386, 6660
BspMI ACCTGC 3 cut(s) 1862, 4594, 5771
BspPI GGATC 9 cut(s) 333, 1516, 1993, 2364, 3075, 3243, 5043, 5592, 6851
BspQI GCTCTTC 1 cut(s) 7754
BspT104I TTCGAA 2 cut(s) 4748, 4940
BspT107I GGYRCC 1 cut(s) 3366
BspTI CTTAAG 1 cut(s) 7460
BspTNI GGTCTC 2 cut(s) 2755, 6430
BsrFI RCCGGY 1 cut(s) 7850
BsrGI TGTACA 1 cut(s) 4722
BssAI RCCGGY 1 cut(s) 7850
BssNI GRCGYC 1 cut(s) 2702
BssSI CACGAG 2 cut(s) 6230, 7331
BssT1I CCWWGG 5 cut(s) 2568, 6648, 6926, 7386, 7623
Bst2BI CACGAG 2 cut(s) 6230, 7331
BstACI GRCGYC 1 cut(s) 2702
BstAFI CTTAAG 1 cut(s) 7460
BstAPI GCANNNNNTGC 2 cut(s) 200, 1877
BstAUI TGTACA 1 cut(s) 4722
BstBI TTCGAA 2 cut(s) 4748, 4940
BstDSI CCRYGG 2 cut(s) 5053, 6926
BstEII GGTNACC 3 cut(s) 1067, 1913, 7246
BstENI CCTNNNNNAGG 2 cut(s) 828, 3784
BstMAI GTCTC 9 cut(s) 2453, 2755, 2777, 2942, 5426, 5921, 6022, 6430, 7894
BstNSI RCATGY 9 cut(s) 610, 1523, 1808, 3506, 3531, 3933, 4667, 5492, 5810
BstPAI GACNNNNGTC 1 cut(s) 3329
BstPI GGTNACC 3 cut(s) 1067, 1913, 7246
BstSFI CTRYAG 8 cut(s) 1583, 3921, 4926, 5241, 5382, 6489, 6656, 7212
BstSLI GKGCMC 3 cut(s) 196, 904, 5569
BstXI CCANNNNNNTGG 3 cut(s) 119, 3033, 7783
Bsu15I ATCGAT 1 cut(s) 7749
BsuI GTATCC 2 cut(s) 144, 5523
BsuTUI ATCGAT 1 cut(s) 7749
BtgI CCRYGG 2 cut(s) 5053, 6926
BtgZI GCGATG 1 cut(s) 3738
BtsI GCAGTG 3 cut(s) 1319, 1677, 6653
BveI ACCTGC 3 cut(s) 1862, 4594, 5771
CaiI CAGNNNCTG 1 cut(s) 3317
CciI TCATGA 2 cut(s) 1545, 3622
Cfr10I RCCGGY 1 cut(s) 7850
ClaI ATCGAT 1 cut(s) 7749
CseI GACGC 2 cut(s) 3717, 7094
CsiI ACCWGGT 2 cut(s) 7078, 7678
DraI TTTAAA 7 cut(s) 220, 502, 2299, 4482, 5107, 6303, 7770
DraIII CACNNNGTG 1 cut(s) 5100
EaeI YGGCCR 5 cut(s) 2565, 3035, 3214, 3742, 5091
EciI GGCGGA 3 cut(s) 3135, 3968, 7483
Ecl136II GAGCTC 2 cut(s) 158, 7764
Eco130I CCWWGG 5 cut(s) 2568, 6648, 6926, 7386, 7623
Eco147I AGGCCT 2 cut(s) 3779, 6637
Eco24I GRGCYC 3 cut(s) 160, 6577, 7766
Eco31I GGTCTC 2 cut(s) 2755, 6430
Eco32I GATATC 4 cut(s) 2923, 3607, 6162, 7747
Eco47I GGWCC 7 cut(s) 33, 818, 1100, 1250, 2066, 2599, 5139
Eco53kI GAGCTC 2 cut(s) 158, 7764
Eco57I CTGAAG 8 cut(s) 945, 1218, 2375, 2762, 3456, 5214, 6171, 7833
Eco88I CYCGRG 2 cut(s) 153, 6860
Eco91I GGTNACC 3 cut(s) 1067, 1913, 7246
EcoICRI GAGCTC 2 cut(s) 158, 7764
EcoNI CCTNNNNNAGG 2 cut(s) 828, 3784
EcoO109I RGGNCCY 2 cut(s) 818, 4696
EcoO65I GGTNACC 3 cut(s) 1067, 1913, 7246
EcoRI GAATTC 3 cut(s) 2194, 5786, 6613
EcoRV GATATC 4 cut(s) 2923, 3607, 6162, 7747
EcoT14I CCWWGG 5 cut(s) 2568, 6648, 6926, 7386, 7623
EcoT22I ATGCAT 2 cut(s) 1953, 5808
EcoT38I GRGCYC 3 cut(s) 160, 6577, 7766
ErhI CCWWGG 5 cut(s) 2568, 6648, 6926, 7386, 7623
FaqI GGGAC 3 cut(s) 19, 841, 2915
FauNDI CATATG 3 cut(s) 235, 1359, 4200
FbaI TGATCA 3 cut(s) 2548, 4129, 6126
FblI GTMKAC 4 cut(s) 2580, 4102, 7393, 7859
FriOI GRGCYC 3 cut(s) 160, 6577, 7766
GsaI CCCAGC 4 cut(s) 8, 384, 614, 7412
GsuI CTGGAG 6 cut(s) 2779, 2936, 2966, 4554, 7072, 7725
HapII CCGG 3 cut(s) 3949, 4517, 7851
HgaI GACGC 2 cut(s) 3717, 7094
Hin1I GRCGYC 1 cut(s) 2702
HincII GTYRAC 5 cut(s) 1194, 1773, 2320, 4103, 7603
HindII GTYRAC 5 cut(s) 1194, 1773, 2320, 4103, 7603
HindIII AAGCTT 5 cut(s) 1885, 2744, 4032, 6512, 6896
HpaII CCGG 3 cut(s) 3949, 4517, 7851
Hpy99I CGWCG 2 cut(s) 1960, 4104
HpyCH4IV ACGT 5 cut(s) 292, 2583, 2702, 4099, 6351
HpySE526I ACGT 5 cut(s) 292, 2583, 2702, 4099, 6351
Hsp92I GRCGYC 1 cut(s) 2702
Ksp22I TGATCA 3 cut(s) 2548, 4129, 6126
LguI GCTCTTC 1 cut(s) 7754
LmnI GCTCC 5 cut(s) 1717, 2014, 2038, 4519, 7102
MabI ACCWGGT 2 cut(s) 7078, 7678
MaeII ACGT 5 cut(s) 292, 2583, 2702, 4099, 6351
MfeI CAATTG 5 cut(s) 1832, 2603, 4254, 4470, 5404
MhlI GDGCHC 7 cut(s) 160, 196, 904, 2043, 5569, 6577, 7766
MlsI TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
MluNI TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
MmeI TCCRAC 9 cut(s) 970, 1094, 2197, 2397, 2577, 3283, 4299, 6891, 7800
Mox20I TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
Mph1103I ATGCAT 2 cut(s) 1953, 5808
MroXI GAANNNNTTC 5 cut(s) 84, 96, 2687, 3347, 7434
MscI TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
MslI CAYNNNNRTG 5 cut(s) 786, 1524, 2885, 7867, 7881
Msp20I TGGCCA 4 cut(s) 2567, 3037, 3744, 5093
MspA1I CMGCKG 2 cut(s) 3548, 6455
MspCI CTTAAG 1 cut(s) 7460
MspI CCGG 3 cut(s) 3949, 4517, 7851
MssI GTTTAAAC 1 cut(s) 6303
MunI CAATTG 5 cut(s) 1832, 2603, 4254, 4470, 5404
Mva1269I GAATGC 8 cut(s) 780, 1244, 1374, 1565, 1832, 1953, 6145, 7259
NciI CCSGG 1 cut(s) 3950
NcoI CCATGG 1 cut(s) 6926
NdeI CATATG 3 cut(s) 235, 1359, 4200
NlaIV GGNNCC 7 cut(s) 35, 819, 1719, 2327, 3368, 3833, 4280
NmeAIII GCCGAG 2 cut(s) 4004, 7871
NsiI ATGCAT 2 cut(s) 1953, 5808
NspI RCATGY 9 cut(s) 610, 1523, 1808, 3506, 3531, 3933, 4667, 5492, 5810
NspV TTCGAA 2 cut(s) 4748, 4940
OliI CACNNNNGTG 1 cut(s) 7867
PaeI GCATGC 2 cut(s) 4667, 5492
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 2 cut(s) 1545, 3622
PaqCI CACCTGC 2 cut(s) 4594, 5771
PceI AGGCCT 2 cut(s) 3779, 6637
PciI ACATGT 3 cut(s) 1519, 1804, 3502
PciSI GCTCTTC 1 cut(s) 7754
PctI GAATGC 8 cut(s) 780, 1244, 1374, 1565, 1832, 1953, 6145, 7259
PdmI GAANNNNTTC 5 cut(s) 84, 96, 2687, 3347, 7434
PflFI GACNNNGTC 1 cut(s) 2124
PflMI CCANNNNNTGG 4 cut(s) 1109, 2063, 3265, 6172
PfoI TCCNGGA 2 cut(s) 727, 2001
PmeI GTTTAAAC 1 cut(s) 6303
PpuMI RGGWCCY 1 cut(s) 818
PscI ACATGT 3 cut(s) 1519, 1804, 3502
PshAI GACNNNNGTC 1 cut(s) 3329
PshBI ATTAAT 1 cut(s) 2982
PsiI TTATAA 4 cut(s) 2081, 2354, 3785, 7041
Psp124BI GAGCTC 2 cut(s) 160, 7766
Psp5II RGGWCCY 1 cut(s) 818
PspEI GGTNACC 3 cut(s) 1067, 1913, 7246
PspFI CCCAGC 4 cut(s) 4, 380, 610, 7408
PspN4I GGNNCC 7 cut(s) 35, 819, 1719, 2327, 3368, 3833, 4280
PspPPI RGGWCCY 1 cut(s) 818
PspXI VCTCGAGB 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 4779, 4811
PstI CTGCAG 2 cut(s) 5386, 6660
PstNI CAGNNNCTG 1 cut(s) 3317
PsyI GACNNNGTC 1 cut(s) 2124
PvuII CAGCTG 2 cut(s) 3548, 6455
RseI CAYNNNNRTG 5 cut(s) 786, 1524, 2885, 7867, 7881
SacI GAGCTC 2 cut(s) 160, 7766
SalI GTCGAC 1 cut(s) 4101
SapI GCTCTTC 1 cut(s) 7754
ScaI AGTACT 5 cut(s) 116, 2166, 2727, 4630, 6961
SduI GDGCHC 7 cut(s) 160, 196, 904, 2043, 5569, 6577, 7766
SexAI ACCWGGT 2 cut(s) 7078, 7678
SfcI CTRYAG 8 cut(s) 1583, 3921, 4926, 5241, 5382, 6489, 6656, 7212
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 4748, 4940
SinI GGWCC 7 cut(s) 33, 818, 1100, 1250, 2066, 2599, 5139
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 5 cut(s) 786, 1524, 2885, 7867, 7881
SmlI CTYRAG 8 cut(s) 153, 1863, 2241, 4106, 4700, 6639, 7460, 7520
SmoI CTYRAG 8 cut(s) 153, 1863, 2241, 4106, 4700, 6639, 7460, 7520
SpeI ACTAGT 1 cut(s) 6190
SphI GCATGC 2 cut(s) 4667, 5492
SseBI AGGCCT 2 cut(s) 3779, 6637
SsiI CCGC 9 cut(s) 203, 1317, 2170, 3120, 3953, 5723, 6599, 7468, 7933
SspI AATATT 6 cut(s) 1174, 1292, 2407, 4563, 5232, 6467
SstI GAGCTC 2 cut(s) 160, 7766
StuI AGGCCT 2 cut(s) 3779, 6637
StyI CCWWGG 5 cut(s) 2568, 6648, 6926, 7386, 7623
TaiI ACGT 5 cut(s) 295, 2586, 2705, 4102, 6354
TspGWI ACGGA 4 cut(s) 1944, 3231, 4388, 5659
Tth111I GACNNNGTC 1 cut(s) 2124
Van91I CCANNNNNTGG 4 cut(s) 1109, 2063, 3265, 6172
Vha464I CTTAAG 1 cut(s) 7460
VneI GTGCAC 2 cut(s) 192, 5565
VpaK11BI GGWCC 7 cut(s) 33, 818, 1100, 1250, 2066, 2599, 5139
VspI ATTAAT 1 cut(s) 2982
XagI CCTNNNNNAGG 2 cut(s) 828, 3784
XbaI TCTAGA 2 cut(s) 1979, 5554
XceI RCATGY 9 cut(s) 610, 1523, 1808, 3506, 3531, 3933, 4667, 5492, 5810
XcmI CCANNNNNNNNNTGG 2 cut(s) 1110, 3209
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 4 cut(s) 2580, 4102, 7393, 7859
XmnI GAANNNNTTC 5 cut(s) 84, 96, 2687, 3347, 7434
ZraI GACGTC 1 cut(s) 2703
ZrmI AGTACT 5 cut(s) 116, 2166, 2727, 4630, 6961
Zsp2I ATGCAT 2 cut(s) 1953, 5808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.