Rh4DG435700

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000223
Physical Location & Seq
Forward (+)
63900844 .. 63907321
6478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG435700.1

Sequence Viewer

Length: 1209 bp
ATGAGGCAAGGGCGTGTGATTAGCGGTGATGTGGCTCTCGTCCATGAGCGATCGTCTCCTCGTTCCGTCGTCGATGGTGGTGTTGGGCGAAACCCTAGAAATGAGTCAACTGGTCAAAGGGGGGAACTTCTCTTCAATGCACGCAGTGCTACATCGTTGATCATCTTTCAATTTGACAAAGAAAAAGCTATTCTGTTTGATCTAATTGATTACATGAAGGTTCAGAAAGCTTCACTGACACTGCTTTCTTGGTCTCTTGAAGACATATGTAATGAAAACCTTTACAAGCACCAGGTTGGAAGCCCTGAATCATTTCAGTCCGACCTTCATCAATATTTTGCATCTTACAAATATCCTTTATTGGAGGACACTCGAGCTCAACTGCAATGCAGGATCGGAACCCTGTATAAAGCACCATTTGGTAAAGTAATTTCTATACAAGAATCCAATCCGCATGGAACAAAGCAATACAGTGTCAAAGTTGATTCTTGGAGAAACAGTGTCATTAAGGAATGCAAAACTAGGCCTGGGGATCTTTTTATTTTAGCAGATGCTAAAGCTGAAAGTGTTTCTGATTTACAAAAGGAAGGGAGATCATGGGTGTTTCTAATAGCCATTGAAGTATTCCCAAAGAGTAGGAGTGAGGATGATGATGGTTTTATCAAGCTCTGCTGCATGAAGTATTTTGAGGGTAACTTGGAAGTTCCAATGATCTGGCCAGCCTCCTTGGATGTCATCCGATATAAGGATCTTTGCGTCACTAGTGATTCAGTGGATGACGTGTCTAAGGATGAAAGTTATGTTGAAAATTCAAAGGTCAGTGAGAGTTTGTTGCTGATGAAATTCTACTCCTTATCATCTGGGCTCATGAATCACTTGCTCTCTGACCTTCAGGGCAGAGAGTTGGTTCTCCCATTTGAAGTTACTGACGAAGAAATGGAGGCCATACTTTACAATAGAAGTACATTCATAGCTGGGCGATCAGGCACTGGGAAGACCACTGTTTTAACAATGAAGTTATTTCAGAAGGAACAGTATTACAATATGGCAGAGGAAGGATTGTATGCTGTCGAGCAGAGTTCTGGAGGTTTTAATGCAACTGTTTTACGTCAGATATTTGTAACAGTTAGTCCAGAACTCTGTATTGCCGTTAAGCAACATGTTTCAAACTTGAAAAGGGACTATGACTTTGGAAAGCTCTGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

402

Amino Acids

45.54

Weight (kDa)

5.58

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 139 - 217 5.7e-21 Domain of unknown function (DUF6469)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 24, 452
AclWI GGATC 3 cut(s) 401, 540, 756
AcoI YGGCCR 1 cut(s) 716
AcsI RAATTY 2 cut(s) 808, 842
AcuI CTGAAG 1 cut(s) 875
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 1 cut(s) 964
AfiI CCNNNNNNNGG 1 cut(s) 745
AflIII ACRYGT 2 cut(s) 780, 1159
AgsI TTSAA 9 cut(s) 136, 170, 260, 620, 806, 813, 920, 1167, 1174
AhlI ACTAGT 1 cut(s) 761
AjiI CACGTC 1 cut(s) 781
AjnI CCWGG 2 cut(s) 291, 526
AluBI AGCT 7 cut(s) 188, 230, 377, 560, 667, 974, 1198
AluI AGCT 7 cut(s) 188, 230, 377, 560, 667, 974, 1198
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 2 cut(s) 60, 258
AlwI GGATC 3 cut(s) 401, 540, 756
AlwNI CAGNNNCTG 1 cut(s) 989
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 3 cut(s) 524, 716, 942
ApeKI GCWGC 1 cut(s) 672
ApoI RAATTY 2 cut(s) 808, 842
ArsI GACNNNNNNTTYG 2 cut(s) 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 372
BalI TGGCCA 1 cut(s) 718
BanII GRGCYC 2 cut(s) 379, 867
BbsI GAAGAC 2 cut(s) 267, 1001
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 659
BccI CCATC 2 cut(s) 68, 647
BceAI ACGGC 1 cut(s) 1133
BciT130I CCWGG 2 cut(s) 293, 528
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 60, 258
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 3 cut(s) 96, 522, 762
BisI GCNGC 1 cut(s) 673
BlsI GCNGC 1 cut(s) 674
Bme1390I CCNGG 2 cut(s) 293, 528
BmeT110I CYCGRG 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 2 cut(s) 293, 528
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 2 cut(s) 350, 541
BmuI ACTGGG 1 cut(s) 999
BpiI GAAGAC 2 cut(s) 267, 1001
BpmI CTGGAG 1 cut(s) 1104
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 527, 726
BsaXI ACNNNNNCTCC 2 cut(s) 484, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 745
Bse1I ACTGG 2 cut(s) 115, 994
Bse3DI GCAATG 1 cut(s) 392
BseBI CCWGG 2 cut(s) 293, 528
BseDI CCNNGG 2 cut(s) 527, 726
BseGI GGATG 5 cut(s) 652, 735, 736, 781, 796
BseLI CCNNNNNNNGG 1 cut(s) 745
BseMI GCAATG 1 cut(s) 392
BseNI ACTGG 2 cut(s) 115, 994
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 659
BseYI CCCAGC 1 cut(s) 974
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 526, 718, 944
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 1193
BslI CCNNNNNNNGG 1 cut(s) 745
BsmAI GTCTC 2 cut(s) 60, 258
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 1193
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 3 cut(s) 526, 718, 944
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 379, 867
Bsp143I GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
BspACI CCGC 2 cut(s) 24, 452
BspANI GGCC 3 cut(s) 526, 718, 944
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 3 cut(s) 401, 540, 756
BspTNI GGTCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 392
BsrI ACTGG 2 cut(s) 115, 994
BssECI CCNNGG 2 cut(s) 527, 726
BssMI GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
BssT1I CCWWGG 1 cut(s) 726
Bst2UI CCWGG 2 cut(s) 293, 528
Bst4CI ACNGT 6 cut(s) 473, 500, 1003, 1035, 1102, 1126
Bst6I CTCTTC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 142, 720
BstDEI CTNAG 1 cut(s) 786
BstF5I GGATG 5 cut(s) 652, 735, 736, 781, 796
BstKTI GATC 9 cut(s) 53, 162, 202, 396, 535, 596, 714, 751, 983
BstMAI GTCTC 2 cut(s) 60, 258
BstMBI GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 293, 528
BstNSI RCATGY 1 cut(s) 1163
BstSCI CCNGG 2 cut(s) 291, 526
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 2 cut(s) 267, 1001
BstX2I RGATCY 2 cut(s) 532, 748
BstXI CCANNNNNNTGG 1 cut(s) 714
BstYI RGATCY 2 cut(s) 532, 748
BsuRI GGCC 3 cut(s) 526, 718, 944
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 5 cut(s) 652, 735, 736, 781, 796
BtsI GCAGTG 2 cut(s) 151, 239
BtsIMutI CAGTG 9 cut(s) 151, 233, 239, 478, 505, 777, 826, 987, 999
Cac8I GCNNGC 2 cut(s) 142, 720
CaiI CAGNNNCTG 1 cut(s) 989
CciI TCATGA 1 cut(s) 867
CseI GACGC 1 cut(s) 745
CsiI ACCWGGT 1 cut(s) 291
Csp6I GTAC 1 cut(s) 963
CviAII CATG 7 cut(s) 44, 214, 455, 597, 676, 868, 1160
CviQI GTAC 1 cut(s) 963
DdeI CTNAG 1 cut(s) 786
DpnI GATC 9 cut(s) 52, 161, 201, 395, 534, 595, 713, 750, 982
DpnII GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
DraIII CACNNNGTG 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 716
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 726
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 2 cut(s) 379, 867
Eco31I GGTCTC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 875
Eco88I CYCGRG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 291, 526
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 2 cut(s) 379, 867
ErhI CCWWGG 1 cut(s) 726
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 7 cut(s) 47, 217, 458, 600, 679, 871, 1163
FaqI GGGAC 1 cut(s) 1193
FatI CATG 7 cut(s) 43, 213, 454, 596, 675, 867, 1159
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 673
FokI GGATG 5 cut(s) 659, 722, 743, 788, 803
FriOI GRGCYC 2 cut(s) 379, 867
Fsp4HI GCNGC 1 cut(s) 673
FspBI CTAG 3 cut(s) 96, 522, 762
GluI GCNGC 1 cut(s) 673
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 1 cut(s) 1104
HaeIII GGCC 3 cut(s) 526, 718, 944
HgaI GACGC 1 cut(s) 745
Hin1II CATG 7 cut(s) 47, 217, 458, 600, 679, 871, 1163
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 6 cut(s) 104, 308, 443, 485, 767, 871
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 8 cut(s) 225, 322, 398, 574, 740, 886, 1026, 1113
Hpy188III TCNNGA 4 cut(s) 257, 868, 1083, 1133
Hpy8I GTNNAC 1 cut(s) 108
Hpy99I CGWCG 2 cut(s) 71, 74
HpyAV CCTTC 6 cut(s) 211, 335, 581, 899, 1021, 1049
HpyCH4III ACNGT 6 cut(s) 473, 500, 1003, 1035, 1102, 1126
HpyCH4IV ACGT 2 cut(s) 780, 1108
HpyCH4V TGCA 7 cut(s) 140, 341, 385, 390, 516, 675, 1097
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 1 cut(s) 786
HpySE526I ACGT 2 cut(s) 780, 1108
Hsp92II CATG 7 cut(s) 47, 217, 458, 600, 679, 871, 1163
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 2 cut(s) 350, 541
MabI ACCWGGT 1 cut(s) 291
MaeI CTAG 3 cut(s) 96, 522, 762
MaeII ACGT 2 cut(s) 780, 1108
MaeIII GTNAC 4 cut(s) 692, 757, 922, 1120
MalI GATC 9 cut(s) 52, 161, 201, 395, 534, 595, 713, 750, 982
MboI GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
MboII GAAGA 4 cut(s) 124, 272, 944, 1006
MflI RGATCY 2 cut(s) 532, 748
MhlI GDGCHC 2 cut(s) 379, 867
MlsI TGGCCA 1 cut(s) 718
MluCI AATT 5 cut(s) 170, 204, 429, 808, 842
MluNI TGGCCA 1 cut(s) 718
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 277, 345
MnlI CCTC 8 cut(s) 69, 358, 637, 682, 733, 934, 1045, 1079
Mox20I TGGCCA 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 312
MscI TGGCCA 1 cut(s) 718
MseI TTAA 4 cut(s) 507, 1007, 1092, 1152
Msp20I TGGCCA 1 cut(s) 718
MspR9I CCNGG 2 cut(s) 293, 528
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 2 cut(s) 293, 528
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeI CATATG 1 cut(s) 266
NdeII GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
NlaIII CATG 7 cut(s) 47, 217, 458, 600, 679, 871, 1163
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 757
NspI RCATGY 1 cut(s) 1163
PaeR7I CTCGAG 1 cut(s) 372
PagI TCATGA 1 cut(s) 867
PceI AGGCCT 1 cut(s) 526
PciI ACATGT 1 cut(s) 1159
PctI GAATGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 5 cut(s) 308, 443, 485, 767, 871
PkrI GCNGC 1 cut(s) 674
Ple19I CGATCG 1 cut(s) 53
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PscI ACATGT 1 cut(s) 1159
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 291, 526
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 2 cut(s) 291, 526
PspN4I GGNNCC 1 cut(s) 400
PspXI VCTCGAGB 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 989
PsuI RGATCY 2 cut(s) 532, 748
PvuI CGATCG 1 cut(s) 53
RsaI GTAC 1 cut(s) 964
RsaNI GTAC 1 cut(s) 963
SacI GAGCTC 1 cut(s) 379
SaqAI TTAA 4 cut(s) 507, 1007, 1092, 1152
SatI GCNGC 1 cut(s) 673
Sau3AI GATC 9 cut(s) 50, 159, 199, 393, 532, 593, 711, 748, 980
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 2 cut(s) 293, 528
SduI GDGCHC 2 cut(s) 379, 867
SexAI ACCWGGT 1 cut(s) 291
SfaNI GCATC 2 cut(s) 350, 541
Sfr274I CTCGAG 1 cut(s) 372
SlaI CTCGAG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 372
SmoI CTYRAG 1 cut(s) 372
SpeI ACTAGT 1 cut(s) 761
Sse9I AATT 5 cut(s) 170, 204, 429, 808, 842
SseBI AGGCCT 1 cut(s) 526
SsiI CCGC 2 cut(s) 24, 452
SspI AATATT 1 cut(s) 335
SspMI CTAG 3 cut(s) 96, 522, 762
SstI GAGCTC 1 cut(s) 379
StuI AGGCCT 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 291, 526
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 6 cut(s) 473, 500, 1003, 1035, 1102, 1126
TaiI ACGT 2 cut(s) 783, 1111
TaqI TCGA 3 cut(s) 72, 373, 1071
TasI AATT 5 cut(s) 170, 204, 429, 808, 842
TatI WGTACW 1 cut(s) 962
TfiI GAWTC 5 cut(s) 308, 443, 485, 767, 871
Tru1I TTAA 4 cut(s) 507, 1007, 1092, 1152
Tru9I TTAA 4 cut(s) 507, 1007, 1092, 1152
TscAI CASTG 9 cut(s) 151, 240, 246, 478, 505, 777, 826, 994, 1006
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 1 cut(s) 672
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 9 cut(s) 230, 288, 317, 692, 807, 854, 884, 958, 1028
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 9 cut(s) 151, 240, 246, 478, 505, 777, 826, 994, 1006
XapI RAATTY 2 cut(s) 808, 842
XceI RCATGY 1 cut(s) 1163
XhoI CTCGAG 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 3 cut(s) 96, 522, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.