Rw2G020880

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000223
Physical Location & Seq
Reverse (-)
28609888 .. 28621556
11669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020880.1

Sequence Viewer

Length: 7335 bp
ATGGAGGGTTCACACAAGAAAAAGCCTAAAAAACCCAACCTTTTGGCTGAGACTGTGTTTTCTTGGTCCCTTGATAACATTTTCAATGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCACTCTGTGGAGCATTACCTCGGGTGTTATCTGTATCCTTTATTGGAAGAAACAAGAGCGCAGGTCCATGCAAGTATGGAAACTATTTACAGAGCACCATTTGCTAAAGTAGTTGCTTTTGAAAAAGTGAAGCCATATGGAACAAAGCTATATGATATCAAGGTTGATTATTGGCGAAACAGGTTAAATGACCGTGGCAAAGAGCCATATGAAACTTTGCCAGGTGATCTTTTTGTTTTGGCAAATGCTAAACCTGAAAGTTTTTCGGATTTACAAAGGGTAGGGAGGTCATGGGCTTTTGCATCAGTCACTAAAGTCTCAGAAAATGAGAACGAGGATGAAAGTACTTCTCTTTATTTTAAAATCAAGGCTTCCAAAGAACTTGAAGTTGTAAAGGGTTCGGCATCACTGTTTATGGTTTTTCTAGTGAACTTAATCCCGAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAACATTATCAAGGAAGTTCTGTGCACTGATTCTGTGGCTCATGAAAATCTCTGCTCTGAAAAGAATAATGACATCGTGAATAAGGGGTTAGTTCAGAGTTTATCATCTGGTCTGAATGAATCCCAAACAGGGACTGTTTTAGCCTGCCTTGAAATGCTGCATTGTCGTGAGAAGTCTGCTGTTGAACTAATTTGGGGTCCTCCTGGTACTGGAAAAACTAAAACAATTGCGACTCTACTTGTAACCTTGTTACAGATGAATTGTAGGACTCTTGTTTGTGCCCCAACAAATGTGGCAATAACTGAAGTTGCTTCTCGTGTTGTAAAGATGTTGACTGAAGTACAGTCCAGTTCTTTGTTTTGTTCTTTCGGAGAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAGAGTTGGCTCAGATATTGAAGAGATATATCTAGATTACCGTCTCAAAAGGCTTGCCGAGTGCTTAGGACCACAGACTGGCTGGAGGCATTGCTTTGCTTCCATGATAGATTTTCTTGAAGATTGTGTTTCTCACTACCATAACTTTTTGGTAAATGAGTTAACCATAGAGAAGGAACCAAAGACCTTAGGTCAAATGAAAGGAAAAGAAAGCAAGTGTGACACTCAAGTTGGCGAGGAGAAGTGCAAATCATTTCTGGAATTTATTAGAGACAGATTTGTTTCTAATGCATCACCTCTAAGAAATTGTATTGCTATCTTATGTACCCACATGGCGAAAAATTACATTTGTGGAGATAATGTCCAAAACATGATTATGCTTATCAACTTGGTTGATTCCTTTGAATCATTGTTGTTTCAAGATAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCTTGTTCTGACATTGACGACATATGGGAGACATATGCTGATAACTCATTTCATCTATTCATGAATAGAAGAGAATGCCTTAAAGTCTTGCACACTCTGTTTGACTCCCTTAGTGAACTTCACATTCCGAATTTTGTGAAGCAAGAGTCTCTAATGGAATTTTGTTTTCAAAATGCCTCCTTGATATTTTGCACTGCATCCAGCTCATATAAGTTGCATAAAGTGGCAATGGAGCCGTTGAACGTTGTTGTTATTGATGAAGCTGCACAATTGAAAGAGTCTGAATCAACAATCCCCCTTCAACTATGGGGTGTGAAGCATGCTGTTCTAGTTGGGGATGAATGTCAGTTACCAGCTACAGTGAAGAGCATTGTATCTGATGAAGCAGGTTTTGCAAGAAGTTTGTTCGAGAGGTTAAGCTCATTGGGTCGCTCGAAACACCTTCTAAATATGCAATACAGGATGCATCCATCAATAAGTTTCTTCCCAAATTCTAGATTCTACAATAACATGATCCTGGATGCTCCAAATCTTAAAAGTAGAAGCCAGAAGAAACACTATCTTAAAGGGTCAATGTTTGGTCCTTATTCTTTCATCAACGTAATTGGTGGACGAGAAGAGAAGGATGAGGATGGACGTAGTCGAAAGAATATGGTGGAGGTTGCTATTGTTTCACAAATACTGCGGAATCTCTACAAAAAATGGGTTGAATCAAAGCAGAAACTCAGTATTGGTATAGTATCTCCATATGCTGCTCAAGTAGTCGCAATCGAGGACAAACTTGCACAGAAGTATAACAATCTTGATGGCTTTACAGTGAAGGTAAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTGTCCACTGTACGATCCAATTATCAGCGGTCACTTGAGTTTGTTTCAAAACCGCAAAGAATTAATGTTGCTCTTACAAGGGCTAGGCACTGTCTATGGATTTTGGGGAATGAAAAAATTCTATGTCATAATGAGTCTGTTTGGAAGGCCGTGGTCCTTGATGCCAAAAATCGCCATTGCTTTTTTAATGTGGATGAAGACAAGGATCTAGCCAAGGCCATATTGGAGGTTAAGAAACAGTACGACCAACTAGATGATTTGCTTGATGCTGACAGTATACTTTTCAGAAGTGCTAGATGGAAGGTGCTTTTTAGTGATAACTTTTTTAAGTCATTCAAGAAACTGACATCAGTCCGTTTGAAGAGGTCGGTACTAAATGTTCTGCTGAAGCTTGCCAGTGGCTGGAGACCAAAGAAGAAGAGTTCAGAAATGATTTGTGGTAGCTCTTCACTGATCTTGAAGAAATTTAAGGTTGAAAATCTGTATGTTGTTTGTACAACAGACATTGCAAAGGACTCAAGATACATTCAAGTATTGAAGATTTGGGACATATTGCCCATAGGGGATGTCCCAACTTTGGTCAATCGCCTGGACCGTATAATGAACAAATATACTGATGCTTTCATTAATCTCTGTAAGGAGAGATGTCTTGAGGGTGATTTGGAAATTCCAAAGAGCTGGCCACCTTCTTTGGAATTTTTGCGGTTTAAGGATTTTAGCTTCACTAAAATCCAGAGTGATTTTGTTGGTGACACATGTGATGGTAGAAGTTACGCTGAGAATTCACGGGTTAGCGAAAGTTTGTTGCTGATGAAGTTCTACTCATTATCATCTGGGGTAGTAAACCACTTGCTGTCTGATTGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACGGACCAAGAAATGGAGATTATCCTTCATCATAGAAGTTCCTTCATAGTTGGAAGGTCAGGAACTGGGAAAACCACTGTTTTGACAATGAAATTATTTCAGAAAGAACAGTGGCACCAGTTGGCAGTGCAAGGATACTATACTAGTCAAAGCAGCAAGATTGAAAAGAGTTCAGCAGCTACTGAGGAGAAAGTTCTACACCAGCTTTTCGTTACAGTGAGTCCTAAGCTGTGTTATGCCATCAAGCAACATGTCTTACACTTGAAAAGTTTTGCATGTGGTGGAAGTCATTCGACTGAAAGCAGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTCAACGATATCCAAGATTCATTTCATGAAATTCCTCCCCATTCTTACCCTCTTTTCATCACATTTCATAAGTTCTTGATTATGCTTGATGGAACACTGAGCAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGCTTACTCATGGTCATCTACGGAATTCAAGATCAGCTGCCTTGCAGACTTTTATAAGAACAAAGGAAGTCAGTTATGAGAGGTTTAGTTCATTATACTGGCCGCATTTTAATACCGATTTAACAAAGAGGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCCCATATAAAAGGTGGCTTGGGAGCTATAGAAGCATGTGATGGTAAACTCAGTCGAGAGGATTATGTACAACTGTCAGAGATCCGAGTTTCCATTCTAACCAGGCAAGAGAGAGAAGTTATATATGATATTTTTCAGATGTATGAAAAAATGAAGATGGAAAATGGGGAATTTGATCTTGCTGATTTTGTAATTGATCTTCACCATCGACTGATGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATGTTGATGAGGTTCAAGATCTAACAATGAGTCAAATTGCCTTGTTTAAACATATGTGCCACAATGTTGAAGATGGTTTTGTTTTTTCCGGTGACACTGCACAAACAATTGCAAGGGGTATTGATTTCAGGTTTCAAGAATTACGACATCTATTCTACAAGAAGTTTGTGTTGGAGTCAAGAACCAATAAGCACGATGAAAGTAAGGAAAAAGGCCAAGTCTCGGAAATTCTTCACTTGACTCAAAACTTTCGTACCCATGATGGAATCCTCAAGCTATCACAAAGCATTATTGACCTACTATATCATTTCTTTCCCCTTTCCATTGATGTCTTAAAACCTGAAAGTAGTTTGATATTTGGGGAAGCTCCGGTATTGCTTGAATCTGAAGAAAATGAAAATGCAATCGTAAAGATTTTTGGAAATACTGGGAATATAGTTGGATTTGGTGCAGAGCAGGTAATTTTGGTCCGAGATGATTGTGCTCGAAAAGAGATATCCAAATTTATAGGGAAGCATGCTCTTGTTCTAACCATTGTGGAGTGCAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTTGGCTCATCACCTTCAAAAAACCAGTGGAGGGTTATATATGATTACATGAATGAACTAGATTTACTCGACCACAGTTTACCCAGGGGCTTTCCTGGTTTCGATGTAGCCAAACACAATATCTTATGCTCTGAACTGAAGCAACTATATGTGGCCGTTACTCGCACGAGACAGAGATTGTGGATTTGTGAAAATATGGAAGAGCTGTCCAAACCAATGTTTGACTACTGGAAGAAGAAATGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGAAATGCAAGTTGCAAGCAGTCCTGAGGAGTGGAAGTCACGTGGCATTAAGCTATATCATGAACACAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCAGGTGACACTTACTGGGAAAGAAGGTCTAAAGCAGCTGGCCTTAAAGCACTTGCTGCCCATATACGAACGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTAACGCTATAGGCAAGGCAGATTCTGCTGCCAGATGTTTTTCTGATCTGGGGGAGTATGAAAGAGCTGCTAGAATATATTTGAAATGTGGGGAGTCTGAACTGAAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGACGATATAAGGAGGCAGCAGATGTCTATGCTAAGGGTAATTTTTTCGCAGACAAGGGCTGGCCTCTGAAGAAGTTTCCACAACAGGAGGCCCTCTTGGCAAAAGCCAAGTCACTTGCCAAGAATGAGACAGACAACTTTCATGAGTTGGTTTGCACTGAGGTTGATATTTTGTTAAATAGGCAGGATAACTTGGCTGTGCTGAAAAATCAATTGAATTCTTCTCAGAGACACCAGAGTATCAGAGGGGAAGTTTTATCTGCTCGAAAAATTTTGGATGCACATCTTTCTCAAAGAACTGATAAGTATATATGGGAAAAAGAATTGATTGGTGATCTAGTAGAGGAGTCAGAAGAGAAAATATCTAAGAATCAGGTTTCCATTGATTCACTGGTATACTTTTGGAATTTTTGGAAGGAGAAGATTCTTCACATATATGAATCTGTGCGATGTTTGGAAACTCCAGAATGCAAGGGATATGTAGAGTGTTGTTTAACTTGCTTAGGTGTGTGTAGGCTATATCATGATCTGAATCCAGTCTATGTGTTGCTCCTCCCTCGCGCTGATTGGGTAAGATGTCTGGAAAGAAGACAATTCCAAAATCACGGGAAGTTGGTCTCCATTGATGGTCAGCAGCTCATTTCAGCTGCTCAGAAATATTGGAGTTCAGAAATGATCTCTGTTGGAATCAAGGTTTTGGAAAAGCTTGAAGCTCTTTACAAGTTCCCTGTCAAGAATTCTGATTCAGCCTTCTTCCAAAGCAAGTCCCTTACCTTGATCTATGATGTTGCAGTGTATCTTGTGGATTCCAAATGTCTGAAGCCTAGGCATCAAGATACCACAACACTACAGAGGTTTGTAACTTTTTCAACTGAGAGTTTGGTGTCTTACATGTTTCCTTTAGATGGGAGGAACTCATTGAGAGAGAGTATGATTTCTCTTAGAAGAACTGATGCTTTAAAGAATGCTCTGAGACAAGTAGTATTTGAATATACCCGGTCAAAGAACAAGCCGTCTTTTGGGGATATTGGAAGGATTGCAATGATCATTCTCGGGACTGGTAAGCTGAATAGCGAACTATATGCTAAGCTTCTCAAAAAGCTAGACTCCCACTCAAATTTGAGGGCCTTCATTATTTTTCTTTGCGAGACTTTTGGACGTGGGGAAATTATTTACGTGAGTATTATGTTGCCATTCCGTAAGGAAGTTAAAGTCAACGGGAGGGTTATTTATGACTACATGTCACCTGGTTGCTTCTTGTATATTCTGGAACGCCTTCTGATTTGGGCAGCTCTTACTCAAGGTTATGTTCTTACTACAAGTTCTTGCTTTATTGAATGGCTGATATCCCAGGATGAAGATAGCCGCTTAAATTTCATTGTGGAAGAAGATCGATCACCTTCTCTCGAAGGCATCCTTGAACTTACGATTAATGACTGTATATCGGAAAAGAAGGATATGATTGAATGGCTTGAAAAGTCTGACAAAAACTGGAAGAAGCATTATCCGCTATACATCCTTCGATTGGTTGTTCTGTTATGTTTGCTTTATGTGAACTTTGGTTTGTCTTTGGATACACTCTTTGACTTTCTGGGGAGGAGCTATGTCGCTGAGCAGTTGCCTCGTGACTTTGTTGACACCCTTAGGAGGGGGTGGAGTCATGATTCCCTTAGGAGGGATAATTCTCCGATTTCAAATGTGAGTGCACTTGCTGCAGCTCTCAAGAAGGATGATAATGATTCGGTGTTTGCAAGTTTTAGGCAGGATTGTAGTTGTACACCTCATAATTCTCTGAGTTCAAATGTGAATGCACTTGCTGCAGCTCTCAAGAAGGTTGATAATGATTTGGTAATTGCAAGTTTTAGGCGGGATTGTTCAAAGTTATGTTGTTCAGATGCCATCTTTATTGACATGACGGCCAACCAGTGCAAGGATGACATACTGAGAAAAATTGTGTCCAAAGCAGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2444

Amino Acids

280.06

Weight (kDa)

6.56

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 77 - 205 8.4e-64 Domain of unknown function (DUF6469)
AAA_11 PF13086 243 - 615 3.1e-35 AAA domain
AAA_19 PF13245 263 - 611 5.2e-07 AAA domain
AAA_12 PF13087 624 - 820 7e-58 AAA domain
UvrD-helicase PF00580 1091 - 1417 1.7e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3779
AarI CACCTGC 1 cut(s) 5099
Acc36I ACCTGC 4 cut(s) 184, 1853, 4588, 5099
AccB1I GGYRCC 1 cut(s) 3357
AccB7I CCANNNNNTGG 3 cut(s) 1097, 2748, 3256
AccI GTMKAC 2 cut(s) 2623, 5757
AccII CGCG 2 cut(s) 5342, 5922
AclI AACGTT 1 cut(s) 1719
AclWI GGATC 6 cut(s) 1984, 2355, 2559, 3234, 3964, 4131
AcoI YGGCCR 5 cut(s) 3026, 3824, 4878, 5085, 7278
AcvI CACGTG 1 cut(s) 5048
AdeI CACNNNGTG 2 cut(s) 139, 5094
AflIII ACRYGT 5 cut(s) 600, 3101, 3493, 6252, 6600
AhdI GACNNNNNGTC 2 cut(s) 1209, 6601
AhlI ACTAGT 1 cut(s) 3386
AjiI CACGTC 1 cut(s) 6521
AjuI GAANNNNNNNTTGG 4 cut(s) 3594, 3626, 4295, 4327
AloI GAACNNNNNNTCC 2 cut(s) 5167, 5199
Alw21I GWGCWC 4 cut(s) 229, 641, 4627, 7069
Alw44I GTGCAC 2 cut(s) 637, 7065
AlwI GGATC 6 cut(s) 1984, 2355, 2559, 3234, 3964, 4131
AlwNI CAGNNNCTG 5 cut(s) 749, 2748, 3308, 3425, 5252
Ama87I CYCGRG 2 cut(s) 152, 6413
ApaLI GTGCAC 2 cut(s) 637, 7065
ArsI GACNNNNNNTTYG 2 cut(s) 5456, 5488
AseI ATTAAT 3 cut(s) 2409, 2973, 6792
Asp700I GAANNNNTTC 7 cut(s) 117, 129, 993, 2463, 3532, 4371, 6636
AspA2I CCTAGG 1 cut(s) 6185
AspLEI GCGC 2 cut(s) 193, 5924
AsuC2I CCSGG 1 cut(s) 6358
AvaI CYCGRG 2 cut(s) 152, 6413
AvaII GGWCC 9 cut(s) 66, 196, 812, 1088, 2057, 2500, 2938, 3247, 4609
AvrII CCTAGG 1 cut(s) 6185
AxyI CCTNAGG 4 cut(s) 1207, 5031, 7004, 7031
BaeGI GKGCMC 3 cut(s) 641, 898, 7069
BaeI ACNNNNGTAYC 4 cut(s) 4378, 4411, 5584, 5617
BalI TGGCCA 2 cut(s) 3028, 5087
BanI GGYRCC 1 cut(s) 3357
BarI GAAGNNNNNNTAC 2 cut(s) 4071, 4103
BauI CACGAG 3 cut(s) 930, 4891, 6984
BbrPI CACGTG 1 cut(s) 5048
BbsI GAAGAC 3 cut(s) 1473, 2550, 5956
Bbv12I GWGCWC 4 cut(s) 229, 641, 4627, 7069
BceAI ACGGC 5 cut(s) 1696, 2480, 4865, 6358, 7293
BcgI CGANNNNNNTGC 4 cut(s) 6425, 6459, 6965, 6999
BciVI GTATCC 3 cut(s) 177, 3371, 6928
BclI TGATCA 1 cut(s) 6405
BcnI CCSGG 1 cut(s) 6358
BcuI ACTAGT 1 cut(s) 3386
BfmI CTRYAG 6 cut(s) 1833, 3915, 5235, 6209, 7074, 7179
BfuAI ACCTGC 4 cut(s) 184, 1853, 4588, 5099
BfuI GTATCC 3 cut(s) 177, 3371, 6928
BglI GCCNNNNNGGC 1 cut(s) 5459
BglII AGATCT 1 cut(s) 4159
BlnI CCTAGG 1 cut(s) 6185
BlpI GCTNAGC 2 cut(s) 6447, 6972
BmcAI AGTACT 1 cut(s) 478
Bme18I GGWCC 9 cut(s) 66, 196, 812, 1088, 2057, 2500, 2938, 3247, 4609
BmeRI GACNNNNNGTC 2 cut(s) 1209, 6601
BmeT110I CYCGRG 2 cut(s) 152, 6413
BmgBI CACGTC 1 cut(s) 6521
BmiI GGNNCC 6 cut(s) 68, 813, 1197, 1710, 2318, 3359
BmrI ACTGGG 3 cut(s) 3318, 4578, 5131
BmuI ACTGGG 3 cut(s) 3318, 4578, 5131
BoxI GACNNNNGTC 1 cut(s) 2696
BpiI GAAGAC 3 cut(s) 1473, 2550, 5956
BpmI CTGGAG 3 cut(s) 1123, 2770, 5808
Bpu10I CCTNAGC 4 cut(s) 1084, 3468, 5394, 5863
Bpu1102I GCTNAGC 2 cut(s) 6447, 6972
BpuMI CCSGG 1 cut(s) 6358
Bsa29I ATCGAT 1 cut(s) 6754
BsaAI YACGTR 2 cut(s) 5048, 6538
BsaBI GATNNNNATC 2 cut(s) 5643, 5892
BsaI GGTCTC 2 cut(s) 2746, 5983
BsaWI WCCGGW 2 cut(s) 4229, 4510
BsaXI ACNNNNNCTCC 4 cut(s) 1365, 1395, 5027, 5057
Bse21I CCTNAGG 4 cut(s) 1207, 5031, 7004, 7031
Bse3DI GCAATG 5 cut(s) 1108, 1710, 2521, 2850, 6408
Bse8I GATNNNNATC 2 cut(s) 5643, 5892
BseCI ATCGAT 1 cut(s) 6754
BseJI GATNNNNATC 2 cut(s) 5643, 5892
BseMI GCAATG 5 cut(s) 1108, 1710, 2521, 2850, 6408
BseRI GAGGAG 6 cut(s) 1271, 3443, 5048, 5720, 5903, 6973
BseSI GKGCMC 3 cut(s) 641, 898, 7069
BsgI GTGCAG 3 cut(s) 1725, 4224, 4611
Bsh1236I CGCG 2 cut(s) 5342, 5922
BshNI GGYRCC 1 cut(s) 3357
BshVI ATCGAT 1 cut(s) 6754
BsiHKAI GWGCWC 4 cut(s) 229, 641, 4627, 7069
BsiHKCI CYCGRG 2 cut(s) 152, 6413
BsiSI CCGG 3 cut(s) 4230, 4511, 6358
BslFI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6112, 6430
BsmBI CGTCTC 2 cut(s) 98, 1067
BsmFI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6112, 6430
BsmI GAATGC 4 cut(s) 1556, 5834, 6331, 7174
Bso31I GGTCTC 2 cut(s) 2746, 5983
BsoBI CYCGRG 2 cut(s) 152, 6413
Bsp1286I GDGCHC 5 cut(s) 229, 641, 898, 4627, 7069
Bsp1407I TGTACA 3 cut(s) 2840, 3955, 7136
Bsp1720I GCTNAGC 2 cut(s) 6447, 6972
BspDI ATCGAT 1 cut(s) 6754
BspFNI CGCG 2 cut(s) 5342, 5922
BspHI TCATGA 7 cut(s) 655, 1536, 3613, 5065, 5503, 5884, 7021
BspLI GGNNCC 6 cut(s) 68, 813, 1197, 1710, 2318, 3359
BspMAI CTGCAG 2 cut(s) 7078, 7183
BspMI ACCTGC 4 cut(s) 184, 1853, 4588, 5099
BspPI GGATC 6 cut(s) 1984, 2355, 2559, 3234, 3964, 4131
BspQI GCTCTTC 3 cut(s) 1835, 2797, 4920
BspT107I GGYRCC 1 cut(s) 3357
BspTNI GGTCTC 2 cut(s) 2746, 5983
BsrDI GCAATG 5 cut(s) 1108, 1710, 2521, 2850, 6408
BsrGI TGTACA 3 cut(s) 2840, 3955, 7136
BssNAI GTATAC 2 cut(s) 2624, 5758
BssSI CACGAG 3 cut(s) 930, 4891, 6984
BssT1I CCWWGG 2 cut(s) 2559, 6185
Bst1107I GTATAC 2 cut(s) 2624, 5758
Bst2BI CACGAG 3 cut(s) 930, 4891, 6984
BstAPI GCANNNNNTGC 6 cut(s) 233, 1868, 5162, 5252, 7073, 7178
BstAUI TGTACA 3 cut(s) 2840, 3955, 7136
BstBAI YACGTR 2 cut(s) 5048, 6538
BstC8I GCNNGC 9 cut(s) 760, 1074, 1797, 2739, 3026, 4659, 5023, 5146, 5423
BstDSI CCRYGG 3 cut(s) 325, 2496, 5340
BstFNI CGCG 2 cut(s) 5342, 5922
BstHHI GCGC 2 cut(s) 193, 5924
BstNSI RCATGY 9 cut(s) 604, 1799, 3105, 3497, 3522, 3927, 4661, 6256, 6604
BstPAI GACNNNNGTC 1 cut(s) 2696
BstSFI CTRYAG 6 cut(s) 1833, 3915, 5235, 6209, 7074, 7179
BstSLI GKGCMC 3 cut(s) 641, 898, 7069
BstUI CGCG 2 cut(s) 5342, 5922
BstV2I GAAGAC 3 cut(s) 1473, 2550, 5956
BstX2I RGATCY 4 cut(s) 2551, 3226, 3969, 4159
BstXI CCANNNNNNTGG 2 cut(s) 43, 3024
BstYI RGATCY 4 cut(s) 2551, 3226, 3969, 4159
BstZ17I GTATAC 2 cut(s) 2624, 5758
Bsu15I ATCGAT 1 cut(s) 6754
Bsu36I CCTNAGG 4 cut(s) 1207, 5031, 7004, 7031
BsuI GTATCC 3 cut(s) 177, 3371, 6928
BsuTUI ATCGAT 1 cut(s) 6754
BtgI CCRYGG 3 cut(s) 325, 2496, 5340
BtgZI GCGATG 1 cut(s) 5824
BtrI CACGTC 1 cut(s) 6521
BtsI GCAGTG 4 cut(s) 1668, 3375, 4236, 6159
BveI ACCTGC 4 cut(s) 184, 1853, 4588, 5099
Cac8I GCNNGC 9 cut(s) 760, 1074, 1797, 2739, 3026, 4659, 5023, 5146, 5423
CaiI CAGNNNCTG 5 cut(s) 749, 2748, 3308, 3425, 5252
CciI TCATGA 7 cut(s) 655, 1536, 3613, 5065, 5503, 5884, 7021
CfoI GCGC 2 cut(s) 193, 5924
Cfr42I CCGCGG 1 cut(s) 5343
ClaI ATCGAT 1 cut(s) 6754
CsiI ACCWGGT 1 cut(s) 6607
CspCI CAANNNNNGTGG 4 cut(s) 888, 923, 3018, 3053
DraI TTTAAA 3 cut(s) 493, 4189, 6321
DraIII CACNNNGTG 2 cut(s) 139, 5094
DriI GACNNNNNGTC 2 cut(s) 1209, 6601
EaeI YGGCCR 5 cut(s) 3026, 3824, 4878, 5085, 7278
Eam1105I GACNNNNNGTC 2 cut(s) 1209, 6601
Eco130I CCWWGG 2 cut(s) 2559, 6185
Eco31I GGTCTC 2 cut(s) 2746, 5983
Eco32I GATATC 4 cut(s) 289, 3598, 4638, 6708
Eco47I GGWCC 9 cut(s) 66, 196, 812, 1088, 2057, 2500, 2938, 3247, 4609
Eco72I CACGTG 1 cut(s) 5048
Eco81I CCTNAGG 4 cut(s) 1207, 5031, 7004, 7031
Eco88I CYCGRG 2 cut(s) 152, 6413
EcoO109I RGGNCCY 4 cut(s) 812, 4690, 5452, 6486
EcoRI GAATTC 4 cut(s) 3127, 3748, 5578, 6097
EcoRV GATATC 4 cut(s) 289, 3598, 4638, 6708
EcoT14I CCWWGG 2 cut(s) 2559, 6185
EcoT22I ATGCAT 3 cut(s) 1312, 1944, 4107
ErhI CCWWGG 2 cut(s) 2559, 6185
Esp3I CGTCTC 2 cut(s) 98, 1067
FalI AAGNNNNNCTT 2 cut(s) 6762, 6794
FaqI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6112, 6430
FauI CCCGC 1 cut(s) 7221
FauNDI CATATG 7 cut(s) 268, 340, 1499, 1510, 2224, 4128, 4194
FbaI TGATCA 1 cut(s) 6405
FblI GTMKAC 2 cut(s) 2623, 5757
GlaI GCGC 2 cut(s) 192, 5923
GsuI CTGGAG 3 cut(s) 1123, 2770, 5808
HapII CCGG 3 cut(s) 4230, 4511, 6358
HhaI GCGC 2 cut(s) 193, 5924
Hin6I GCGC 2 cut(s) 191, 5922
HinP1I GCGC 2 cut(s) 191, 5922
HincII GTYRAC 5 cut(s) 948, 1182, 2311, 6577, 6997
HindII GTYRAC 5 cut(s) 948, 1182, 2311, 6577, 6997
HindIII AAGCTT 4 cut(s) 591, 2735, 6065, 6449
HpaI GTTAAC 1 cut(s) 1182
HpaII CCGG 3 cut(s) 4230, 4511, 6358
HpyCH4IV ACGT 8 cut(s) 91, 1719, 2076, 2113, 5047, 5177, 6520, 6537
HpySE526I ACGT 8 cut(s) 91, 1719, 2076, 2113, 5047, 5177, 6520, 6537
HspAI GCGC 2 cut(s) 191, 5922
Ksp22I TGATCA 1 cut(s) 6405
KspAI GTTAAC 1 cut(s) 1182
KspI CCGCGG 1 cut(s) 5343
LguI GCTCTTC 3 cut(s) 1835, 2797, 4920
LmnI GCTCC 8 cut(s) 142, 1012, 1708, 2005, 3911, 4513, 5916, 6960
MabI ACCWGGT 1 cut(s) 6607
MaeII ACGT 8 cut(s) 91, 1719, 2076, 2113, 5047, 5177, 6520, 6537
MfeI CAATTG 4 cut(s) 840, 1745, 4248, 5573
MflI RGATCY 4 cut(s) 2551, 3226, 3969, 4159
MhlI GDGCHC 5 cut(s) 229, 641, 898, 4627, 7069
MlsI TGGCCA 2 cut(s) 3028, 5087
MluNI TGGCCA 2 cut(s) 3028, 5087
MmeI TCCRAC 4 cut(s) 3274, 4293, 4561, 6025
Mox20I TGGCCA 2 cut(s) 3028, 5087
Mph1103I ATGCAT 3 cut(s) 1312, 1944, 4107
MroXI GAANNNNTTC 7 cut(s) 117, 129, 993, 2463, 3532, 4371, 6636
MscI TGGCCA 2 cut(s) 3028, 5087
MslI CAYNNNNRTG 3 cut(s) 780, 1394, 5796
Msp20I TGGCCA 2 cut(s) 3028, 5087
MspA1I CMGCKG 5 cut(s) 2374, 3761, 5144, 5342, 6008
MspI CCGG 3 cut(s) 4230, 4511, 6358
MssI GTTTAAAC 1 cut(s) 4189
MunI CAATTG 4 cut(s) 840, 1745, 4248, 5573
Mva1269I GAATGC 4 cut(s) 1556, 5834, 6331, 7174
MvnI CGCG 2 cut(s) 5342, 5922
NciI CCSGG 1 cut(s) 6358
NdeI CATATG 7 cut(s) 268, 340, 1499, 1510, 2224, 4128, 4194
NlaIV GGNNCC 6 cut(s) 68, 813, 1197, 1710, 2318, 3359
NmeAIII GCCGAG 1 cut(s) 1102
NsiI ATGCAT 3 cut(s) 1312, 1944, 4107
NspI RCATGY 9 cut(s) 604, 1799, 3105, 3497, 3522, 3927, 4661, 6256, 6604
PaeI GCATGC 2 cut(s) 1799, 4661
PagI TCATGA 7 cut(s) 655, 1536, 3613, 5065, 5503, 5884, 7021
PaqCI CACCTGC 1 cut(s) 5099
PasI CCCWGGG 1 cut(s) 4809
PciI ACATGT 5 cut(s) 600, 3101, 3493, 6252, 6600
PciSI GCTCTTC 3 cut(s) 1835, 2797, 4920
PcsI WCGNNNNNNNCGW 1 cut(s) 2938
PctI GAATGC 4 cut(s) 1556, 5834, 6331, 7174
PdmI GAANNNNTTC 7 cut(s) 117, 129, 993, 2463, 3532, 4371, 6636
PflFI GACNNNGTC 1 cut(s) 2115
PflMI CCANNNNNTGG 3 cut(s) 1097, 2748, 3256
PfoI TCCNGGA 1 cut(s) 1992
PmaCI CACGTG 1 cut(s) 5048
PmeI GTTTAAAC 1 cut(s) 4189
PmlI CACGTG 1 cut(s) 5048
Ppu21I YACGTR 2 cut(s) 5048, 6538
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 5 cut(s) 600, 3101, 3493, 6252, 6600
PshAI GACNNNNGTC 1 cut(s) 2696
PshBI ATTAAT 3 cut(s) 2409, 2973, 6792
PsiI TTATAA 1 cut(s) 3779
Psp1406I AACGTT 1 cut(s) 1719
Psp5II RGGWCCY 1 cut(s) 812
PspCI CACGTG 1 cut(s) 5048
PspN4I GGNNCC 6 cut(s) 68, 813, 1197, 1710, 2318, 3359
PspPPI RGGWCCY 1 cut(s) 812
PsrI GAACNNNNNNTAC 4 cut(s) 2709, 2741, 4773, 4805
PstI CTGCAG 2 cut(s) 7078, 7183
PstNI CAGNNNCTG 5 cut(s) 749, 2748, 3308, 3425, 5252
PsuI RGATCY 4 cut(s) 2551, 3226, 3969, 4159
PsyI GACNNNGTC 1 cut(s) 2115
PvuII CAGCTG 3 cut(s) 3761, 5144, 6008
RseI CAYNNNNRTG 3 cut(s) 780, 1394, 5796
SacII CCGCGG 1 cut(s) 5343
SapI GCTCTTC 3 cut(s) 1835, 2797, 4920
ScaI AGTACT 1 cut(s) 478
SduI GDGCHC 5 cut(s) 229, 641, 898, 4627, 7069
SexAI ACCWGGT 1 cut(s) 6607
SfcI CTRYAG 6 cut(s) 1833, 3915, 5235, 6209, 7074, 7179
Sfr303I CCGCGG 1 cut(s) 5343
SgrBI CCGCGG 1 cut(s) 5343
SinI GGWCC 9 cut(s) 66, 196, 812, 1088, 2057, 2500, 2938, 3247, 4609
SmiMI CAYNNNNRTG 3 cut(s) 780, 1394, 5796
SpeI ACTAGT 1 cut(s) 3386
SphI GCATGC 2 cut(s) 1799, 4661
SspI AATATT 2 cut(s) 5226, 6020
StyI CCWWGG 2 cut(s) 2559, 6185
TaiI ACGT 8 cut(s) 94, 1722, 2079, 2116, 5050, 5180, 6523, 6540
TatI WGTACW 5 cut(s) 476, 955, 2840, 3955, 7136
TauI GCSGC 3 cut(s) 3829, 5342, 6729
TspGWI ACGGA 4 cut(s) 2690, 3260, 3760, 6548
Tth111I GACNNNGTC 1 cut(s) 2115
Van91I CCANNNNNTGG 3 cut(s) 1097, 2748, 3256
VneI GTGCAC 2 cut(s) 637, 7065
VpaK11BI GGWCC 9 cut(s) 66, 196, 812, 1088, 2057, 2500, 2938, 3247, 4609
VspI ATTAAT 3 cut(s) 2409, 2973, 6792
XbaI TCTAGA 2 cut(s) 1051, 1970
XceI RCATGY 9 cut(s) 604, 1799, 3105, 3497, 3522, 3927, 4661, 6256, 6604
XcmI CCANNNNNNNNNTGG 4 cut(s) 1098, 2566, 3899, 5749
XmaJI CCTAGG 1 cut(s) 6185
XmiI GTMKAC 2 cut(s) 2623, 5757
XmnI GAANNNNTTC 7 cut(s) 117, 129, 993, 2463, 3532, 4371, 6636
ZrmI AGTACT 1 cut(s) 478
Zsp2I ATGCAT 3 cut(s) 1312, 1944, 4107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.