Rh2DG272400

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000223
Physical Location & Seq
Reverse (-)
30214081 .. 30240114
26034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG272400.1

Sequence Viewer

Length: 8034 bp
ATGGGCAAGCTCTCCAAGGCTGGGAAGAAGATACGACGCCAAGCTCTGCCAGCTTCTCTATGGCCAGAGAGGTCAAATGGTCTAAGGGGAGAAGAACGTCCCCTCCAAGCACAAACCTTTTCTATTTGCATTCACTCTCTCAAGGGTTCTGGTCAGAAATTTTACCTCTCTCTTGAATATCTGGATGGTAATTCCAAGCCCAAAGAGTTGAAATATAACAAAAGCAAGAGAGTTTTGAGTCAGACAAGAAAAAGCAAGGAGATGATGGAAGGTTCACACAAGAAAAAGCCTAAAAAACCCAACCTTTTGGCTGAGACTGTGTTTTCTTGGTCCCTTGATAACATTTTCAATGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCACTCTGTGGAGCATTACTTCGGGTGTTATCTGTATCCTTTATTGGAAGAAACAAGAGCACAGGTCCATGCAAGTATGGAAACTATTTACAGAGCACCATTTGCTAAAGTAGTTGCTTTTGAAAAAGCGAAGCCATATGGAACAAAGCTATATGATATCAAGGTTGATTATTGGCGAAACAGGTTAAATGACCGTGGCAAAGAGCCATATGAAACTTTGCCAGGTGATCTTTTTGTTTTGGCAAATGCTAAACCTGAAAGTTTTTCAGATTTACAAAGGGTAGGGAGGTCATGGGCTTTTGCATCAGTCACTAAAGTCTCAGACAATGAGAACGAGGATGAAAGTACTTCTCTTTATTTTAAAATCAAGGCTTCCAAAGAACTTGAAGTTGTAAAGGGTTCGGCATCACTGTTTATGGTTTTTCTAGTGAACTTAATCCCGAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAACATTATCAAGGAAGTTCTGTGCACTGATTCTGTGGCTCATGAAAATCTCTGCTCTGAAAAGAATAATGACATCGTGAATAAGGGGTTAGTTCAGAGTTTATCATCTGGTCTGAATGAATCCCAAACAGAAACTGTTTTAGCCTGCCTTGAAATGCTGCATTGTCGTGAAAAGTCTGCTGTTGAACTAATTTGGGGTCCTCCTGGTACTGGAAAAACTAAAACAATTGCGACTCTACTTGTAACCTTGTTACAGATGAATTGTAGGACTCTTGTTTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCATGTTGTAAAGATGTTGACTGAAGTAGAGTCCAGTTCTTTGTTTTGTTCTTTTGGAGAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAGAGTTGGCTCAGACATTGAAGAGATATATCTAGATTACCGTCTCAAAAGGCTTTCTGAGTGCTTAGGACCACAGACTGGCTGGAGGCATTGCTTTGCTTCCATGATAGATTTTCTTGAAGATTGTGTTTCTCACTACCATAACTTTTTGGTAAATGAGTTAACCATAGAGAAGGAACCAAAGACCTTAGGTCAAATGAAAGGAAAAGAAAGCAAGTGTGACACTCAAGTTGGCGAGGAGAAGTGCAAATCATTTCTGGAATTTATTAGAGACAGATTTGTTTCTAATGCATCACCTCTAAGAAATTGTATTTCTATCTTATGTACCCACATGGCGAAAAATTACATTTGTGGAGATAATGTCCAAAACATGATTATGCTTATCAACTTGGTTGATTCCTTTGAATCATTGTTGTTTCAAAATAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCTTGTTCTGACATTGACGACATATGGGAGACATATGCTGATAACTCATTTCTTCTATTCATGAATAGAAGAGAATGCCTTAAAGTCTTGCACACTCTGTTTGACTCCCTTAGTGAACTTCACATTCCGAATTTTGTGAAGCAAGAGTCTCTAATGGAATTTTGTTTTCAAAAAGCCTCCTTGATATTTTGCACTGCATCCAGCTCATATAAATTGCATAAAGTGGCAATGGAGCCGTTGAGCGTTGTTGTTATTGATGAAGCTGCACAATTGAAAGAGTCTGAATCAACAATCCCCCTTCAACTATGGGGTGTGAAGCATGCTGTTCTAGTTGGGGATGAATGTCAGTTACCAGCTACAGTGAAGAGCATTGTATCTGATGAAGCAGGTTTTGCAAGAAGTTTGTTCGAGAGGTTAAGCTCATTTGGTCGCTCGAAACACCTTCTAAATATGCAATACAGGATGCATCCATCAATAAGTTTCTTGCCAAATTCTAGATTCTACAATAACATGATCCTGGATGCTCCAAATCTTAAAAGTAGAAGCCAGAAGAAACACTATCTTAAAGGGTCAATGTTTGGTCCTTATTCTTTCATCAATGTAATTGGTGGACGAGAAGAGAAGGATGAGGATGGACGTAGTCGAAAGAATATGGTGGAGGTTGCTATTGTTTCACAAATACTGCGGAATCTCTACAAAAAATGGGTTGAATCAAAGCAGAAACTCAGTATTGGTATAGTATCTCCATATGCTGCTCAAGTAGTCGCAATCGAGGACAAACTTGCACAGAAGTATAATAATCTTGATGGCTTTACAGTGAAGGTAAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTGTCCACTGTACGATCCAATTATCAGCGGTCACTTGAGTTTGTTTCAAAACCGCAAAGAATTAATGTTGCTCTTACAAGGGCTAGGCACTGTCTTTGGATTTTGGGGAATGAAAAAATTCTATGTGATAATGAGTCTGTTTGGAAGGCTGTGGTCCTTGATGCCAAAAATCGCCATTGCTTTTTTAATGCGGATGAAGACAAGGATCTAGCCAAGGCCATATTGGAGGTTAAGAAACAGTATGACCAACTAGATGATTTGCTTGATGCTGACAGTATACTTTTCAGAAGTGCTAGATGGAAGGTGCTTTTTAGTGATAACTTTTTTAAGTCATTCAAGAAACTGACGTCAGTCCGTTTGAAGAGGTCGGTACTAAATGTTCTACTGAAGCTTGCCAGTGGCTGGAGACCAAAGAAGAAGAGTTCAGAAATGATTTGTGGTAGCTCTTCACTGATCTTGAAGAAATTTAAGGTTGAAAATCTGTATGTTGTTTGTACAACAGACATTGCAAAGGACTCGAGATACATTCAAGTATTGAAGATTTGGGACATATTGCCTATAGGGGATGTCCCAACTTTGGTCAATCGCCTGGACCGTATAATGAACAAATATACTGATGCTTTCATTAATCTCTGCAAGGAGAAATGTCTTGAGGGTGATTTGGAAATTCCAAAGAGCTGGCCACCTTCTTTGGAGTTTTTGCGGTTTAAGGATCTTAGCTTCACTAAAATCCAGAATGATTTTGTTGGTGACACATGTGATGGTAGAAGCTACGCTGAGAATTCACGGGTCAGCGAAAGTTTGTTGCTGATGAAGTTCTACTCATTATCATCTGGGGTAGTAAACCACTTGCTGTCTGATTGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACGGACCAAGAAATGGAGATTATCCTTCATCATAGAAGTTCCTTCATAGTTGGAAGGTCAGGAACTGGGAAAACCACTGTTTTAACAATGAAATTATTTCAGAAAGAACAGTGGCACCAGTTGGCAGTGCAAGGATGCTATACTAGTCAAAGCAGCAAGATTGAAAAGAGTTCAGCAGCTACTGAGGAGAAAGTTCTACACCAGCTTTTCGTTACAGTGAGTCCTAAGCTGTGTTATGCCATCAAGCAACATGTCTTACACTTGAAAAGTTTTGCATGTGGTGGAAGTCATTCGACTGAAAGCAGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTCAACGATATCCAAGATTCATTTCATGAAATTCCTCCCCATTCTTACCCTCTTTTCATCACATTTCATAAGTTCTTGATTATGCTTGATGGAACACTGAGCAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGCTTACTCATGGTCATCTACGGAATTCAAGATCAGCTGCCTTGCATACTTTTATAAGAACAAAGGAAGTCAGTTATGAGAGGTTTAGTTCATTATACTGGCCGCATTTTAATACCGATTTAACAAAGAGGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCCCATATAAAAGGTGGCTTGGGAGCTATAGAAGCATGTGATGGTAAACTCAGTCGAGAGGATTATGTACAACTGTCAGAGATCCGAGTTTCCATTCTAACCAGACAAGAGAGAGAAGTTATATATGATATTTTTCAGATGTATGAAAAAATGAAGATGGAAAATGGGGAATTTGATCTTGCTGATTTTGTAATTGATCTTCACCATCGACTGATGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATGTTGATGAGGTTCAAGATCTAACAATGAGTCAAATTGCCTTGTTTAAACATATGTGCCACAATGTTGAAGATGGTTTTGTTTTTTCCGGTGACACTGCACAAACAATTGCAAGGGGTATTGATTTCAGGTTTCAAGAATTACGACATCTATTCTACAAGAAGTTTGTGTTGGAGTCAAGAACCAATAAGCACGATGAAAGTAAAGAAAAAGGCCAAGTCTCGGAAATTCTTCACTTGACTCAAAACTTTCGTACCCATGATGGAATCCTCAAGCTATCACAAAGCATTATTGACCTACTATATCATTTCTTTCCCCTTTCCATTGATGTCTTAAAACCTGAAAGTAGTTTGATATTTGGGGAAGCTCCGGTATTGCTTGAATCTGAAGAAAATGAAAATGCAATCGTAAAGATTTTTGGAAATACTGGGAATATAGTTGGATTTGGTGCAGAGCAGGTAATTTTGGTCCGAGATGATAGTGCTCGAAAAGAGATATCCAAATTTATAGGGAAGCATGCTCTTGTTCTAACCATTGTGGAGTGCAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTTGGCTCATCACCTTCAAAAAACCAGTGGAGGGTTATATATGATTACATGAATGAACTAGATTTACTCGACCACACTTTACCCAGGGGCTTTCCTGGTTTCGATGTAGCCAAACACAACATCTTATGCTCTGAACTGAAGCAACTATATGTGGCTGTTACTCGCACGAGACAGAGATTGTGGATTTGTGAAAATATGGAAGAGCTTTCCAAACCAATGTTTGACTACTGGAAGAAGAAATGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGAAATGCAAGTTGCAAGCAGTCCTGAGGAGTGGAAGTCACGTGGCATTAAGCTATATCATGAACACAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCAGGTGACACTTACTGGGAAAGAAGGTCTAAAGCAGCTGGCCTTAAAGCACTTGCTGCCCATATACGAATGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTAACGCTATAGGCAAGGCAGATTCTGCTGCCAGATGTTTTTCTGATCTGGGGGAGTATGAAAAAGCTGCTAGAATATATTTGAAATGTGGGGAGTCTGAACTGAAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGACGATATAAGGAGGCAGCAGATGTCTATGCTAAGGGTAATTTTTTCGCAGAGTGTCTCACTATTTGTGAAAAGGGAAAACTATTTGAACTGGGTTTGCAGTACATCAATAACTGGAAACAACATGCAGAAGAGGACACTGCTATGACTACTAGAGGAAAAGGAATAAATAGAATGCAGCTTGAATTTTTAGAGAGCTGTGCACTTCACTATTATGATTTGAATGATAACAGATCCATGATTAAGTTTATTAAAGCTTTTCCTTCTATAACTTTGGTGCGGGACTTTTTGAAGAGGTTGGATAGCCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCAAACATCGCACAGCTTAAAGGTGAAATTCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAAGCCTCATTGAAAATAATATTCTACGTACTTGCCATGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCTGAAGAAGTTTCCACAACAGGAGGCCCTCTTGGCAAAAGCCAAGTCACTTGCCAAGAATGAGACAGACAACTTTCATGAGTTGGTTTGCACTGAGGTTGATATTTTATTAAATAGGCAGGATAACTTGGCTGTGCTGAAAAATCAATTGAATTCTTCTCAGAGACACCAGAGTATCAGAGGGGAAGTTTTATCTGCTCGAAAAATTTTGGATGCACATCTTTCTCAAAGAACTGATAAGTATATATGGGAAAAAGAATTGATTGGTGATCTTGTAGAGGAGTCAGAAGAGAAAATATCTAAAAATCAGGTTTCCATTGATTCACTGGTATACTTTTGGAATTTTTGGAAGGAGAAGATTCTTCACATATATGAATCTGTGCGATGTTTGGAAACTCCAGAATGCAAGGGATATGTAGAGTGTTGTTTAACTTGCTTAGGTGTGTGTAGGCTATATCATGATCTGAATCCAGTCTATGTGTTGCTCCTCCCTCGTGCTGATTGGGTAAGATGTCTGGAAAGAAGACAATTCCAAAATCACGGGAAGTTGGTCTCCATTGATGGTCAGCAGCTCACTTCAGCTGCTCAGAAATATTGGAGTTCAGAAATGATCTCTGTTGGAATCAAGGTTTTGGAAAAGCTTGAAGCTCTTTACAAGTTCCCTGTCAAGAATTCTGATTCAGCCTTCTTCCAAAGCAAGTCCCTTACCTTGATCTATGATGTTGCAGTGTATCTTGTGGATTCCAAATGTCTGAAGCCTAGGCATCAAGATACCATAACACTACAGAGGTTTGTAACTTTTTCAACTGAGAGTTTGGTGTCTTACATGTTTCCTTTAGATGGGAGGAACTCATTGAGAGAGAGTATGATTTCTCTTAGAAGAACTGATGCTTTAAAGAATGCTCTGAGACAAGTAGTATTTGAATATACCCGGTCAAAGAACAAGCCGTCTTTTGGGGATATTGGAAGGATTGCAATGATCATTCTCGGGACTGGTAAGCTGAATAGCGAACTATATGCTAAGCTTCTCAAAAAGCTAGACTCCCACTCAAATTTGAGGGCCTTCATTATTTTTCTTTGCGAGACTTTTGGACGTGGGGAAATTATTTACGTGAGTATTATGTTGCCATTCCGTAAGGAAGTTAAAGTCAACGGGAGGGTTATTTATGACTACATGTCACCTGGTTGCTTCTTGTATATTCTGGAACGCCTTCTGATTTGGGCAGCTCTTACTCAAGGTTATGTTCTTACTACAAGTTCTTGCTTTATTGAATGGCTGATATCCCAGGATGAAGATAGCCGCTTAAATTTCATTGTGGAAGAAGATCGATCACCTTCTCTCGAAGGCATCCTTGAACTTACGATTAATGACTGTATATCGGAAAAGAAGGATATGATTGAATGGCTTGAAAAGTCTGACAAAAACTGGAAGAAGCATTATCCGCTATACATCCTTCGATTGGTTGTTCTGTTATGTTTGCTTTATGTGAACTTTGGTTTGTCTTTGGATACACTCTTTGACTTTCTGGGGAGGAGCTATGTCGCTGAGCAGTTGCCTTGTGACTTTGTTGACACCCTTAGGAGGGGGTGGAGTCATGATTCCCTTAGGAGGGATGATTCTCCGATTTCAAATGTGAGTGCACTTGCTGCAGCTCTCAAGAAGGATGATAATGATTCGGTGTTTGCAAGTTTTAGGCAGGATTGTAGTTGTACACCTCATAATTCTCTGAGTTCAAATGTGAATGCACTTGCTGCAGCTCTCAAGAAGGTTGATAATGATTTGGTAATTGCAAGTTTTAGGCGGGATTGTTCAAAGTTATGTTGTTCAGATGCCATCTTTATTGACATGACGGCCAACCAGTGCAAGGATGACATACTGAGAAAAATTGTGTCCAAAGCAGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2677

Amino Acids

306.55

Weight (kDa)

6.77

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 165 - 293 7.7e-64 Domain of unknown function (DUF6469)
AAA_11 PF13086 331 - 703 2.3e-34 AAA domain
AAA_19 PF13245 351 - 699 1.8e-06 AAA domain
AAA_12 PF13087 712 - 908 8.2e-57 AAA domain
AAA_19 PF13245 1177 - 1508 8.7e-06 AAA domain
UvrD-helicase PF00580 1179 - 1505 1.9e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4043
AarI CACCTGC 1 cut(s) 5363
AatII GACGTC 1 cut(s) 2960
Acc36I ACCTGC 4 cut(s) 2117, 4852, 5363, 6032
AccB1I GGYRCC 1 cut(s) 3621
AccB7I CCANNNNNTGG 3 cut(s) 1361, 3012, 3520
AccI GTMKAC 2 cut(s) 2887, 6456
AccII CGCG 1 cut(s) 5606
AclWI GGATC 8 cut(s) 2248, 2619, 2823, 3330, 3498, 4228, 4395, 5853
AcoI YGGCCR 5 cut(s) 62, 3290, 4088, 5349, 7977
AcvI CACGTG 1 cut(s) 5312
AcyI GRCGYC 2 cut(s) 37, 2957
AdeI CACNNNGTG 2 cut(s) 403, 5358
AflIII ACRYGT 5 cut(s) 864, 3365, 3757, 6951, 7299
AhdI GACNNNNNGTC 2 cut(s) 1473, 7300
AhlI ACTAGT 1 cut(s) 3650
AjiI CACGTC 1 cut(s) 7220
AjnI CCWGG 9 cut(s) 616, 1081, 2256, 2583, 3198, 5072, 5083, 7306, 7410
AjuI GAANNNNNNNTTGG 4 cut(s) 3858, 3890, 4559, 4591
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
Alw21I GWGCWC 6 cut(s) 457, 493, 905, 4891, 5830, 7768
Alw44I GTGCAC 3 cut(s) 901, 5826, 7764
AlwI GGATC 8 cut(s) 2248, 2619, 2823, 3330, 3498, 4228, 4395, 5853
AlwNI CAGNNNCTG 5 cut(s) 1013, 3012, 3572, 3689, 5516
Ama87I CYCGRG 2 cut(s) 3127, 7112
ApaLI GTGCAC 3 cut(s) 901, 5826, 7764
ArsI GACNNNNNNTTYG 2 cut(s) 6155, 6187
AseI ATTAAT 3 cut(s) 2673, 3237, 7491
Asp700I GAANNNNTTC 7 cut(s) 381, 393, 1257, 2727, 3796, 4635, 7335
AspA2I CCTAGG 1 cut(s) 6884
AsuC2I CCSGG 1 cut(s) 7057
AvaI CYCGRG 2 cut(s) 3127, 7112
AvaII GGWCC 9 cut(s) 330, 460, 1076, 1352, 2321, 2764, 3202, 3511, 4873
AvrII CCTAGG 1 cut(s) 6884
AxyI CCTNAGG 4 cut(s) 1471, 5295, 7703, 7730
BaeGI GKGCMC 4 cut(s) 905, 1162, 5830, 7768
BaeI ACNNNNGTAYC 4 cut(s) 4642, 4675, 6283, 6316
BalI TGGCCA 3 cut(s) 64, 3292, 5351
BanI GGYRCC 1 cut(s) 3621
BarI GAAGNNNNNNTAC 2 cut(s) 4335, 4367
BauI CACGAG 2 cut(s) 5155, 6618
BbrPI CACGTG 1 cut(s) 5312
BbsI GAAGAC 3 cut(s) 1737, 2814, 6655
Bbv12I GWGCWC 6 cut(s) 457, 493, 905, 4891, 5830, 7768
BceAI ACGGC 3 cut(s) 1960, 7057, 7992
BcgI CGANNNNNNTGC 4 cut(s) 3108, 3142, 7124, 7158
BciT130I CCWGG 9 cut(s) 618, 1083, 2258, 2585, 3200, 5074, 5085, 7308, 7412
BciVI GTATCC 2 cut(s) 441, 7627
BclI TGATCA 1 cut(s) 7104
BcnI CCSGG 1 cut(s) 7057
BcuI ACTAGT 1 cut(s) 3650
BfmI CTRYAG 7 cut(s) 2097, 3168, 4179, 5499, 6908, 7773, 7878
BfuAI ACCTGC 4 cut(s) 2117, 4852, 5363, 6032
BfuI GTATCC 2 cut(s) 441, 7627
BglI GCCNNNNNGGC 1 cut(s) 6158
BglII AGATCT 1 cut(s) 4423
BlnI CCTAGG 1 cut(s) 6884
BlpI GCTNAGC 2 cut(s) 7146, 7671
BmcAI AGTACT 1 cut(s) 742
Bme18I GGWCC 9 cut(s) 330, 460, 1076, 1352, 2321, 2764, 3202, 3511, 4873
BmeRI GACNNNNNGTC 2 cut(s) 1473, 7300
BmeT110I CYCGRG 2 cut(s) 3127, 7112
BmgBI CACGTC 1 cut(s) 7220
BmiI GGNNCC 6 cut(s) 332, 1077, 1461, 1974, 2582, 3623
BmrI ACTGGG 4 cut(s) 3582, 4842, 5395, 5726
BmuI ACTGGG 4 cut(s) 3582, 4842, 5395, 5726
BoxI GACNNNNGTC 1 cut(s) 2960
BpiI GAAGAC 3 cut(s) 1737, 2814, 6655
BpmI CTGGAG 3 cut(s) 1387, 3034, 6507
Bpu10I CCTNAGC 4 cut(s) 1348, 3732, 5658, 6562
Bpu1102I GCTNAGC 2 cut(s) 7146, 7671
BpuMI CCSGG 1 cut(s) 7057
Bsa29I ATCGAT 1 cut(s) 7453
BsaAI YACGTR 3 cut(s) 5312, 6082, 7237
BsaBI GATNNNNATC 2 cut(s) 6342, 6591
BsaHI GRCGYC 2 cut(s) 37, 2957
BsaI GGTCTC 2 cut(s) 3010, 6682
BsaWI WCCGGW 2 cut(s) 4493, 4774
BsaXI ACNNNNNCTCC 6 cut(s) 87, 117, 1629, 1659, 5291, 5321
Bse21I CCTNAGG 4 cut(s) 1471, 5295, 7703, 7730
Bse3DI GCAATG 5 cut(s) 1372, 1974, 2785, 3114, 7107
Bse8I GATNNNNATC 2 cut(s) 6342, 6591
BseBI CCWGG 9 cut(s) 618, 1083, 2258, 2585, 3200, 5074, 5085, 7308, 7412
BseCI ATCGAT 1 cut(s) 7453
BseJI GATNNNNATC 2 cut(s) 6342, 6591
BseMI GCAATG 5 cut(s) 1372, 1974, 2785, 3114, 7107
BseRI GAGGAG 6 cut(s) 1535, 3707, 5312, 6419, 6602, 7672
BseSI GKGCMC 4 cut(s) 905, 1162, 5830, 7768
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 3 cut(s) 1989, 4488, 4875
Bsh1236I CGCG 1 cut(s) 5606
BshNI GGYRCC 1 cut(s) 3621
BshVI ATCGAT 1 cut(s) 7453
BsiHKAI GWGCWC 6 cut(s) 457, 493, 905, 4891, 5830, 7768
BsiHKCI CYCGRG 2 cut(s) 3127, 7112
BsiSI CCGG 3 cut(s) 4494, 4775, 7057
BslFI GGGAC 7 cut(s) 84, 316, 3164, 3170, 5921, 6811, 7129
BsmBI CGTCTC 2 cut(s) 362, 1331
BsmFI GGGAC 7 cut(s) 84, 316, 3164, 3170, 5921, 6811, 7129
BsmI GAATGC 6 cut(s) 129, 1820, 5805, 6533, 7030, 7873
Bso31I GGTCTC 2 cut(s) 3010, 6682
BsoBI CYCGRG 2 cut(s) 3127, 7112
Bsp1286I GDGCHC 7 cut(s) 457, 493, 905, 1162, 4891, 5830, 7768
Bsp1407I TGTACA 3 cut(s) 3104, 4219, 7835
Bsp1720I GCTNAGC 2 cut(s) 7146, 7671
BspDI ATCGAT 1 cut(s) 7453
BspFNI CGCG 1 cut(s) 5606
BspHI TCATGA 7 cut(s) 919, 1800, 3877, 5329, 6202, 6583, 7720
BspLI GGNNCC 6 cut(s) 332, 1077, 1461, 1974, 2582, 3623
BspMAI CTGCAG 2 cut(s) 7777, 7882
BspMI ACCTGC 4 cut(s) 2117, 4852, 5363, 6032
BspPI GGATC 8 cut(s) 2248, 2619, 2823, 3330, 3498, 4228, 4395, 5853
BspQI GCTCTTC 3 cut(s) 2099, 3061, 5184
BspT107I GGYRCC 1 cut(s) 3621
BspTNI GGTCTC 2 cut(s) 3010, 6682
BsrDI GCAATG 5 cut(s) 1372, 1974, 2785, 3114, 7107
BsrGI TGTACA 3 cut(s) 3104, 4219, 7835
BssNAI GTATAC 2 cut(s) 2888, 6457
BssNI GRCGYC 2 cut(s) 37, 2957
BssSI CACGAG 2 cut(s) 5155, 6618
BssT1I CCWWGG 4 cut(s) 15, 2823, 6031, 6884
Bst1107I GTATAC 2 cut(s) 2888, 6457
Bst2BI CACGAG 2 cut(s) 5155, 6618
Bst2UI CCWGG 9 cut(s) 618, 1083, 2258, 2585, 3200, 5074, 5085, 7308, 7412
BstACI GRCGYC 2 cut(s) 37, 2957
BstAPI GCANNNNNTGC 6 cut(s) 497, 2132, 5426, 5516, 7772, 7877
BstAUI TGTACA 3 cut(s) 3104, 4219, 7835
BstBAI YACGTR 3 cut(s) 5312, 6082, 7237
BstDSI CCRYGG 2 cut(s) 589, 5604
BstENI CCTNNNNNAGG 1 cut(s) 6035
BstFNI CGCG 1 cut(s) 5606
BstNI CCWGG 9 cut(s) 618, 1083, 2258, 2585, 3200, 5074, 5085, 7308, 7412
BstPAI GACNNNNGTC 1 cut(s) 2960
BstSFI CTRYAG 7 cut(s) 2097, 3168, 4179, 5499, 6908, 7773, 7878
BstSLI GKGCMC 4 cut(s) 905, 1162, 5830, 7768
BstSNI TACGTA 1 cut(s) 6082
BstUI CGCG 1 cut(s) 5606
BstV2I GAAGAC 3 cut(s) 1737, 2814, 6655
BstX2I RGATCY 6 cut(s) 2815, 3322, 3490, 4233, 4423, 5858
BstXI CCANNNNNNTGG 2 cut(s) 307, 3288
BstYI RGATCY 6 cut(s) 2815, 3322, 3490, 4233, 4423, 5858
BstZ17I GTATAC 2 cut(s) 2888, 6457
Bsu15I ATCGAT 1 cut(s) 7453
Bsu36I CCTNAGG 4 cut(s) 1471, 5295, 7703, 7730
BsuI GTATCC 2 cut(s) 441, 7627
BsuTUI ATCGAT 1 cut(s) 7453
BtgI CCRYGG 2 cut(s) 589, 5604
BtgZI GCGATG 2 cut(s) 5974, 6523
BtrI CACGTC 1 cut(s) 7220
BtsI GCAGTG 5 cut(s) 1932, 3639, 4500, 5763, 6858
BveI ACCTGC 4 cut(s) 2117, 4852, 5363, 6032
CaiI CAGNNNCTG 5 cut(s) 1013, 3012, 3572, 3689, 5516
CciI TCATGA 7 cut(s) 919, 1800, 3877, 5329, 6202, 6583, 7720
Cfr42I CCGCGG 1 cut(s) 5607
ClaI ATCGAT 1 cut(s) 7453
CseI GACGC 1 cut(s) 45
CsiI ACCWGGT 1 cut(s) 7306
CspCI CAANNNNNGTGG 2 cut(s) 3282, 3317
DraI TTTAAA 3 cut(s) 757, 4453, 7020
DraIII CACNNNGTG 2 cut(s) 403, 5358
DriI GACNNNNNGTC 2 cut(s) 1473, 7300
EaeI YGGCCR 5 cut(s) 62, 3290, 4088, 5349, 7977
Eam1105I GACNNNNNGTC 2 cut(s) 1473, 7300
Eco105I TACGTA 1 cut(s) 6082
Eco130I CCWWGG 4 cut(s) 15, 2823, 6031, 6884
Eco31I GGTCTC 2 cut(s) 3010, 6682
Eco32I GATATC 4 cut(s) 553, 3862, 4902, 7407
Eco47I GGWCC 9 cut(s) 330, 460, 1076, 1352, 2321, 2764, 3202, 3511, 4873
Eco72I CACGTG 1 cut(s) 5312
Eco81I CCTNAGG 4 cut(s) 1471, 5295, 7703, 7730
Eco88I CYCGRG 2 cut(s) 3127, 7112
EcoNI CCTNNNNNAGG 1 cut(s) 6035
EcoO109I RGGNCCY 4 cut(s) 1076, 4954, 6151, 7185
EcoRI GAATTC 4 cut(s) 3391, 4012, 6277, 6796
EcoRII CCWGG 9 cut(s) 616, 1081, 2256, 2583, 3198, 5072, 5083, 7306, 7410
EcoRV GATATC 4 cut(s) 553, 3862, 4902, 7407
EcoT14I CCWWGG 4 cut(s) 15, 2823, 6031, 6884
EcoT22I ATGCAT 3 cut(s) 1576, 2208, 4371
ErhI CCWWGG 4 cut(s) 15, 2823, 6031, 6884
Esp3I CGTCTC 2 cut(s) 362, 1331
FalI AAGNNNNNCTT 2 cut(s) 7461, 7493
FaqI GGGAC 7 cut(s) 84, 316, 3164, 3170, 5921, 6811, 7129
FauI CCCGC 2 cut(s) 5898, 7920
FauNDI CATATG 7 cut(s) 532, 604, 1763, 1774, 2488, 4392, 4458
FbaI TGATCA 1 cut(s) 7104
FblI GTMKAC 2 cut(s) 2887, 6456
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 3 cut(s) 1387, 3034, 6507
HapII CCGG 3 cut(s) 4494, 4775, 7057
HgaI GACGC 1 cut(s) 45
Hin1I GRCGYC 2 cut(s) 37, 2957
HincII GTYRAC 5 cut(s) 1212, 1446, 2575, 7276, 7696
HindII GTYRAC 5 cut(s) 1212, 1446, 2575, 7276, 7696
HindIII AAGCTT 5 cut(s) 855, 2999, 5880, 6764, 7148
HpaI GTTAAC 1 cut(s) 1446
HpaII CCGG 3 cut(s) 4494, 4775, 7057
Hpy99I CGWCG 1 cut(s) 39
HpyCH4IV ACGT 8 cut(s) 97, 355, 2377, 2957, 5311, 6081, 7219, 7236
HpySE526I ACGT 8 cut(s) 97, 355, 2377, 2957, 5311, 6081, 7219, 7236
Hsp92I GRCGYC 2 cut(s) 37, 2957
Ksp22I TGATCA 1 cut(s) 7104
KspAI GTTAAC 1 cut(s) 1446
KspI CCGCGG 1 cut(s) 5607
LguI GCTCTTC 3 cut(s) 2099, 3061, 5184
LmnI GCTCC 8 cut(s) 406, 1276, 1972, 2269, 4175, 4777, 6615, 7659
MabI ACCWGGT 1 cut(s) 7306
MaeII ACGT 8 cut(s) 97, 355, 2377, 2957, 5311, 6081, 7219, 7236
MfeI CAATTG 4 cut(s) 1104, 2009, 4512, 6272
MflI RGATCY 6 cut(s) 2815, 3322, 3490, 4233, 4423, 5858
MhlI GDGCHC 7 cut(s) 457, 493, 905, 1162, 4891, 5830, 7768
MlsI TGGCCA 3 cut(s) 64, 3292, 5351
MluNI TGGCCA 3 cut(s) 64, 3292, 5351
MmeI TCCRAC 6 cut(s) 3538, 4557, 4825, 5904, 5931, 6724
Mox20I TGGCCA 3 cut(s) 64, 3292, 5351
Mph1103I ATGCAT 3 cut(s) 1576, 2208, 4371
MroXI GAANNNNTTC 7 cut(s) 381, 393, 1257, 2727, 3796, 4635, 7335
MscI TGGCCA 3 cut(s) 64, 3292, 5351
MslI CAYNNNNRTG 5 cut(s) 1044, 1658, 5768, 5838, 6495
Msp20I TGGCCA 3 cut(s) 64, 3292, 5351
MspA1I CMGCKG 5 cut(s) 2638, 4025, 5408, 5606, 6707
MspI CCGG 3 cut(s) 4494, 4775, 7057
MssI GTTTAAAC 1 cut(s) 4453
MunI CAATTG 4 cut(s) 1104, 2009, 4512, 6272
Mva1269I GAATGC 6 cut(s) 129, 1820, 5805, 6533, 7030, 7873
MvaI CCWGG 9 cut(s) 618, 1083, 2258, 2585, 3200, 5074, 5085, 7308, 7412
MvnI CGCG 1 cut(s) 5606
NciI CCSGG 1 cut(s) 7057
NdeI CATATG 7 cut(s) 532, 604, 1763, 1774, 2488, 4392, 4458
NlaIV GGNNCC 6 cut(s) 332, 1077, 1461, 1974, 2582, 3623
NsiI ATGCAT 3 cut(s) 1576, 2208, 4371
PaeI GCATGC 2 cut(s) 2063, 4925
PaeR7I CTCGAG 1 cut(s) 3127
PagI TCATGA 7 cut(s) 919, 1800, 3877, 5329, 6202, 6583, 7720
PaqCI CACCTGC 1 cut(s) 5363
PasI CCCWGGG 1 cut(s) 5073
PciI ACATGT 5 cut(s) 864, 3365, 3757, 6951, 7299
PciSI GCTCTTC 3 cut(s) 2099, 3061, 5184
PcsI WCGNNNNNNNCGW 1 cut(s) 3202
PctI GAATGC 6 cut(s) 129, 1820, 5805, 6533, 7030, 7873
PdmI GAANNNNTTC 7 cut(s) 381, 393, 1257, 2727, 3796, 4635, 7335
PflFI GACNNNGTC 1 cut(s) 2379
PflMI CCANNNNNTGG 3 cut(s) 1361, 3012, 3520
PfoI TCCNGGA 1 cut(s) 2256
PmaCI CACGTG 1 cut(s) 5312
PmeI GTTTAAAC 1 cut(s) 4453
PmlI CACGTG 1 cut(s) 5312
Ppu21I YACGTR 3 cut(s) 5312, 6082, 7237
PpuMI RGGWCCY 1 cut(s) 1076
PscI ACATGT 5 cut(s) 864, 3365, 3757, 6951, 7299
PshAI GACNNNNGTC 1 cut(s) 2960
PshBI ATTAAT 3 cut(s) 2673, 3237, 7491
PsiI TTATAA 1 cut(s) 4043
Psp5II RGGWCCY 1 cut(s) 1076
Psp6I CCWGG 9 cut(s) 616, 1081, 2256, 2583, 3198, 5072, 5083, 7306, 7410
PspCI CACGTG 1 cut(s) 5312
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 9 cut(s) 616, 1081, 2256, 2583, 3198, 5072, 5083, 7306, 7410
PspN4I GGNNCC 6 cut(s) 332, 1077, 1461, 1974, 2582, 3623
PspPPI RGGWCCY 1 cut(s) 1076
PsrI GAACNNNNNNTAC 4 cut(s) 2973, 3005, 5037, 5069
PstI CTGCAG 2 cut(s) 7777, 7882
PstNI CAGNNNCTG 5 cut(s) 1013, 3012, 3572, 3689, 5516
PsuI RGATCY 6 cut(s) 2815, 3322, 3490, 4233, 4423, 5858
PsyI GACNNNGTC 1 cut(s) 2379
PvuII CAGCTG 3 cut(s) 4025, 5408, 6707
RseI CAYNNNNRTG 5 cut(s) 1044, 1658, 5768, 5838, 6495
SacII CCGCGG 1 cut(s) 5607
SapI GCTCTTC 3 cut(s) 2099, 3061, 5184
ScaI AGTACT 1 cut(s) 742
SduI GDGCHC 7 cut(s) 457, 493, 905, 1162, 4891, 5830, 7768
SexAI ACCWGGT 1 cut(s) 7306
SfcI CTRYAG 7 cut(s) 2097, 3168, 4179, 5499, 6908, 7773, 7878
Sfr274I CTCGAG 1 cut(s) 3127
Sfr303I CCGCGG 1 cut(s) 5607
SgrBI CCGCGG 1 cut(s) 5607
SinI GGWCC 9 cut(s) 330, 460, 1076, 1352, 2321, 2764, 3202, 3511, 4873
SlaI CTCGAG 1 cut(s) 3127
SmiMI CAYNNNNRTG 5 cut(s) 1044, 1658, 5768, 5838, 6495
SnaBI TACGTA 1 cut(s) 6082
SpeI ACTAGT 1 cut(s) 3650
SphI GCATGC 2 cut(s) 2063, 4925
SspI AATATT 3 cut(s) 5490, 6075, 6719
StyI CCWWGG 4 cut(s) 15, 2823, 6031, 6884
TaiI ACGT 8 cut(s) 100, 358, 2380, 2960, 5314, 6084, 7222, 7239
TatI WGTACW 5 cut(s) 740, 3104, 4219, 5727, 7835
TauI GCSGC 3 cut(s) 4093, 5606, 7428
TspGWI ACGGA 4 cut(s) 2954, 3524, 4024, 7247
Tth111I GACNNNGTC 1 cut(s) 2379
Van91I CCANNNNNTGG 3 cut(s) 1361, 3012, 3520
VneI GTGCAC 3 cut(s) 901, 5826, 7764
VpaK11BI GGWCC 9 cut(s) 330, 460, 1076, 1352, 2321, 2764, 3202, 3511, 4873
VspI ATTAAT 3 cut(s) 2673, 3237, 7491
XagI CCTNNNNNAGG 1 cut(s) 6035
XbaI TCTAGA 2 cut(s) 1315, 2234
XcmI CCANNNNNNNNNTGG 5 cut(s) 57, 1362, 2830, 4163, 6448
XhoI CTCGAG 1 cut(s) 3127
XmaJI CCTAGG 1 cut(s) 6884
XmiI GTMKAC 2 cut(s) 2887, 6456
XmnI GAANNNNTTC 7 cut(s) 381, 393, 1257, 2727, 3796, 4635, 7335
ZraI GACGTC 1 cut(s) 2958
ZrmI AGTACT 1 cut(s) 742
Zsp2I ATGCAT 3 cut(s) 1576, 2208, 4371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.