FvH4_5g34990

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000223
Physical Location & Seq
Forward (+)
25574080 .. 25585739
11660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34990.t1

Sequence Viewer

Length: 7869 bp
ATGGAAGCTTCACACAAGAAGAAGGGTAAAAGACCCAACCTTTTGGCCGATACAGTCTTCTCTTGGTCCCTTGATGACGTTCTCAACGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAGTCATTTAACTCTGTTGGGCATTACTTTGGGTGTTATCTGTATCCTTTATTGGAAGAAACACGTGCACAAGTCCATTTGAGTATGGAGACTATTCACAGAGCACCATATGCTAAAGTAGTTTCTTTTGAAAATGCCAAGCCATATGGAAGAAAGCTCTATAATATCAAGGTTGATTACTGGCGAAACAGGTTCAATGACCATGGCAAGGAGCAGTATAAAACTTTGCCTGGTGATCTTTTTGTTTTAGCAAATGCTAAACCTGAAACTGTTTCAGATTTACAAAGGGTTGGGAGGTCGTGGGCTTTTGGATCAGTCACTAAAGTGTCAGAAAATGATGACACTACTTCTCTTTATTTTAAAGTCAAGGCTTCCAAAGAACTTGAAGTCTTAAAGAGCACAACACCATTGTTTATGGTTTTCCTAGTGAACTTAATACCAAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAAGATTATCAAGGAGGTTCTGTGCCCTGATTCTGAGGCTCAGAAAAGTCTCTTCTCTGAAAAGAACAATGACATGGTGAATATGGGATTAGTTCAGAGTTTATCATCTGGTTTGAATGAATCCCAAACTGGGACCGTTTTGGCCTGTCTTGAAATGCTGCATTCTCATGAGAAGTCTGCTGTGGAACTGATTTGGGGTCCTCCTGGTACTGGAAAGACTAAAACTATTGTCACCCTACTTTTAACCCTGCTACAGATGAATTGTAGGACTCTTGTCTGTGCCCCAACAAATGTTGCAATAACTGAAATTGCTTCTCGCCTTGTAAAGATGGTGACTGAAGTAGAGTCCAATGCTCTGTATTGTTCTTTGGCAAAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAAAGTTGGTTCAGACGTTGAAGAGATATATTTAGATTACCGTCTCAAAAGGCTTGCTGAGTGCTTAGGGCCACAGACTGGTTGGAGGCATTGCTTTACTTCCATGATAGAATGTCTTGAAGATGGTGTTTCTCACTACCATACTTTCTTGGAAAACGGATTAACTATTGAGAAGGAACCGAAGACTACAGGTCAAATGAAAGAGAAAGAAAGCAGAATTGTCACTAAAGTGGGCAAGGACAAGTGCAAAACATTTCTGGAGTTTATTAGAGATAGATTTGTTTCTACTGCATCACCTCTAAGATATTGCATTTCTATCTTCTGTACCCACATGGCGAACAATCACATTTCCGTAGATATTTTCCAAAACATGGTTTTGCTTGTCAACTTGGTAGATTCCTTTGAATCATTGTTGTTTCAAGGTAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCTCGTTCTGATGTAGAAGACATATCAGAGACATGTGTTGATAACTCGTTTCTTCTATTCTCAAATAGAAGAGAATGCCTTCAAGCCTTGCATAAACTATTTGACTCTCTTAGAAAACTTGATCTTCCGGACTTTATGAAGCAAGAGTCTCTAATGGCATTTTGTTTTCAAAGTTCTTCCTTGATATTTTGCACTGCATCCAGCTCATATAAGTTGCACAAACTGGCAATGCAGCCACTAAGCATTGTGGTAATTGATGAAGCTGCTCAATTGAAAGAGTGTGAATCAACAATCCCCCTTCAACTACCAGGTGTGAGGCATGCTGTTCTTGTTGGTGACGAATGTCAGTTACCTGCTATAGTGAAGAGCATTGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCGTAGTTGGTCACTCAAAACACCTCCTGAATGTGCAGTACAGGATGCATCCATCAATCAGTTCCTTCCCAAATTCTAGTTTCTACAATAACATGATCCTGGATGCTCCATATATTAAAAGGAGAGGCCAGGAGAAAAATTATCTTAAAGGGTCAATGTTTGGTCCTTACTCTTTTATCAATGTAATTGGTGGACGAGAAGAGAAAGACGAGGATGGACGCAGTCGGAAGAATATGGTTGAGGTTGCTGTTGTTTCACAAATACTGCGGAATTTGTACAAAGAATGGATTGATTCAAAACAGAATCTCAGTATTGGTGTAGTTTCTCCATATGCTGCTCAAGTAGTCGCAATAGAGGACAAACTTGGACAGAAATATAATAATCTTGATGGATTTATAGTGAAAGTGAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTTTCCACTGTACGATCCAATTGTCAACAGTCACTTGAGTTTATTTCAAAACCGCAGAGAATTAATGTTGCTCTTACAAGGGCAAGGCACTGCCTGTGGATTTTGGGGAATGAAAGAACTCTATGTGAAAGTGAGTCTGTTTGGGAGGCCTTGGTCCTTGATGCCAAAAATCGCCAATGCTTTTTTAATGCTGATGAAGACGAGGATTTAGCCAAGGCCATACTACAAGTGAAGAAACAGTTCGACCAACTAGATGATTTGCTTAATTCTGACAGTGTACTTTTCAGAAGAGCTAGATGGAAGGTACTTTTTAGTGATAACTTTTTGAAGTCATTCAAGAAACTGAAATCAGTCAGTTTGAAGAGGTCAGTGCTAAATGTTCTACTAAAGCTTGCCAGTGGCTGGAGACCGAAGATGCATAGTTCAGATATACTTTGTGGTAACTCTTCACTGATCCTGAAGAAATTTAAGGTTGATGACCTATACATTGTTTGCACAACAGACATAGCAAAGGATTTTAGATACATTCAAGTATTGAAGATTTGGGACATAATGCCTATAGAGGATATCCCAGCGTTGGTAAATCGCCTGGACAATATATTAAACAAATATACAGATGAGTATATTAATCTGTGTAAGGAGAAATGTCTCGAGGGTGATTTGGAAGTTCCAAAGAGCTGGCCAAGTTCTTTGGAGTTTTTGCGGTTTAAGGATCTTAGCTGCACTGACATCCAAAATGATTTAGTTGGTGACACTTCTGATGGTCGAAGTTATGTGGAGAATTCAAGGGTCAGCGAAAGTTTGTTACTGATGAAATTTTACTCGCTATCATCTGGCGTTGTAAACCACTTGCTGTCTGATCATGAGGGTATAGAACTGGATCTTCCTTTTGAAGTTACAGACCAAGAAATGGAGATTATCCTTTATCAAAGAAGTTCCTTTATAGTTGGACGGTCAGGAACTGGGAAAACAACAGTTTTAACAATGAAATTATATCAGAAAGAACAGTGGCACCAGTTGGCTGTGCAAGGATGCCAGCTTAGTGACAGCAGCAAGGTTGAACAGATTTCTGCAGACACTGAGGGGAAAGTTCTTCACCAGCTTTTCGTGACAGTGAGTCCAAAGCTCTGTTATGCTATCAAGCAACATGTTTTACACTTGAAAAGATTTGCATCTGGTGGAGGTCATTCAACTGAAACCACTTTGATTGATATGAGTGATTTTGATGAGGATGCAGCTCAATTCAACGATCTCCATGATTCATTTCATGAGGTTCCTCCCCATTCTTACCCTCTTTTCATCACATTGCAGAAGTTCTTAATTATGCTTGATGGAACATTGAGAAACTCGTACTTTGAAAGATTCCTTGATGCAACTGAGGTTACTAATGGTCAAGTACGAAGTTCAAGATCAGTTGCTTTGCAGACATTTATAAGAACAAAGGAAGTCAGTTATGAGAAGTTTAGCTCAATATACTGGCCACATTTTGATACCGAGTTAACAAAGAAGCTTCATGCTTCAAGAGTCTTCACGGAGATCATTTCTCATATAAAAGGTGGCCTGGGAGCTATTGAAGCATGTAATGGTAAACTCAGTCGAGAGGATTATGTTCAACTGTCAGAGAACCGAGCTTCCAGTCTAACTAGCCAAGAGAGAGAAGTTATATATGATATCTTTCAGATGTATGAAAAAATGAAGATGGAACATGGTGAATTTGATCTTTCTGATTTTGTAACTGATCTTCACCGTCGACTCAGGCATGAAAAGTATGAGGGGGATCACATGGACTTTGTTTATATTGATGAGGTTCAGGATCTCACCATGAGTCAGATTGCCTTGTTTAAACACATGTGCCACAATGTTGAAGAGGGGTTTGTATTTTCAGGTGATACTGCACAGACAATTGCAAAGGGTGTTGATTTCAGGTTTCAAGAATTACGACATCTATTCTATAAGAAGTTTGTGCTGGAGTCAAGAAGCAATAAGCACGATGAAAGTAAGGAAAAAGGACAAATCTCAGAGATTCTTCACCTGACTCAAAACTTCCGTACCCATGCTGGAATCCTCAAGCTATCACAAAGCATTGTTGAACTTCTTTATCATTTTTTCCCCTCTTCCATTGATGTTTTAAAACCTGAAAGCAGTTTGATATATGGGGAAGCTCCAGTTTTGCTTGAATCTGGAGAAAATGAAAATGCAATCGGGAAGATTTTTGGAAATACTGGAAATATAGTTGGATTTGGAGCAGAGCAGGTAATTTTGGTGCGAGATGATAGTGCTCGAAATGAGATTTTAAAACTTGTAGGGAAGCATGCTCTTGTGCTTACAATTGTCGAGTGCAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTCGGCTCATCACCTTCGAGAAATCAGTGGAGGGTTATATATGATTACATGAAGGAACTAGATTTACTTGACCACACATTACCTAGGGCGTTTCCAAGTTTTAATGTGGCCAATCACAACATATTGTGCTCTGAACTGAAACAACTATACGTGGCTGTTACTCGCACGAGACAGAGATTGTGGATTTGTGAGAATTTTGAAGAGCTCTCCAAACCAATGTTTGACTACTGGAAGAAGAAATGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGCAATGCAAGTGGCAAGCAGTCCAGAGGAGTGGAAGTCACGTGGCATGAAGCTATATCATGAACATAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGACAGTTACTGGGAAAGAAGGTCTAAAGCTGCTGGCCTTAAAGCACTTGCTGACCATATGCGAACATCAAATCCTGAAGAGGCTAGATGTATACTGAGGGAAGCTGCTGAAATCTTTGATGCTATAGGCAAGGCCGATTCTGCTGCGAGATGCTTTTCTGATTTGGGGGAGTTTGAAAGAGCTGCTAGGATATACTTGAACAGATGTGGTGAGTCTGAACTGGAGAAGGCCGCAGAATGTTTTTCTCTAGCAGGATGCTATGAGGAGGCTGCAGATGTGTATGCTAAGGGGAACTTTTTCTCAGAGTGTCTCACTGTTTGTGCAAAGGGAAAACTATTTGAAATGGGCTTGCAATACATCAATAACTGGAAACAGCATGCAGGAGAGGTCACTGCCATGATTACAAGAGGAAACGGAATAAATAGAATGGAGCATGAATTTTTAGAGAGCTGTGCTGTTCACTATTACAATTTGCATGATAACCGATCCATGATGAAGTTTGTTAAAGCTTTCCATTCTATTATTTTGATGCGAAACTTTTTGAAGAGGTTGGATAGCCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCGAACATTGCACAGCTAAAAGGTGATATTCTGCTTGAATCTGATTTCCTTGGAAAGGCAGGAAAGTTCAAAGAGGCCTCATTGCACATACTGTTCTTAGTACTTGCCGAGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCCGAAGACGTTTCCACAACAGGAGGTGCTTTTGGCAGAAGCCAAGTCACTTGCCAAGAATGAGACAGACATCTTTTATGAGGTGGTTTGCACTGAGGTTGATATCCTATTAAGTGGGATGGATAGCTTGGCTGTTTTGAGAAATCAACTGGAAGCTTCTGTGAGACATATGAGTATCAGAGGGGAAGTGCTATCTGCTCGAAGAATTTTGGATGCCCATCTTTCTCGAAGAACTGATAAGTATGTATGGGAAAAAGACTTGATTGGTGATCTTGTACAGCATTCAGAAGAGATAATATCTAAGAATCAAGTTTCCATAGACTCACTCATCTACTTCTGGAATTCTTGGAAGGAGAAGATCCTTCAAATAATTGAATCTGTAGGTTGTTTGGAAACACAGGACTACAAGGGTTATGTAGAGTTTGGTTTTGCTTTTTTAGGAGTATGGAGGTTAAATCATAATACGAGTCCAGTCTATGTTTTACTTATCTCTGATGCTGATTGGGTTAGAGGTCTGGACAAAATACATTTCCAAAATTATGGGAAGTTAGTCTCCATTGATGTTCACCAACTCATTCGAGCTGCTCAAACTTATTGGGGTTCAGAACTGGTCACTGTTGGCATCAAGGTATTGGAGAAGCTTGAAGCACTTTTCAAGTTCCCAATCAACAGTCCTGATTCAATTTTCTTTCAAAGCAGGGTTCTTACTCTAATCAATGATGTTGGAATGTATCTTTTGGATTCCAACTGTCTGAAACAAAGGAATCAAGATACCATGACATTACAGAGGTTTGTAACCTTTTCGACTGAAAAATATATTACGTGCATATTTCCTTTAGATTGGAGGAATTCAGCGAGAGAGAATATGATTTCTCTCAGAAGAACTTATGCTTCCCAATCTGTGCTGAAACAAGCTATAGTTAAATGTATCAGCGGGAAGAACAAGATGTCTTATGGGCAAATTGGAAAGGTCATCATGATCATTCTTGGGTCTGGTAAGCCAAGTAGTGAACTTCACAGCATGCTTATGAAAAAGCTGGTCGACAACCCGCCATGGATGTCACTCATTGAGACTTTGTATGAGGAAATGGGACCTTGGAGTCGTAGTGGAGAACCAAAACAGGTGTCAGTTTTGTGGCGATTCTATGAAGCATTAGTCAAAACTTATAGTGCAAACTGGAGGGGTCTTCATGATTACATATCTCCTGGTTGCTTCTTGTATCTTGTAGAGCGCCTTTTGACTTTGGCATCTTGCTTTCATGGTTTTGTAATGTGCACCAGTTCAAATTTTATAGAATGGCTGATCTACAAGGATGGAGATACCACCACAAGTTCACCATCTCTGGAACGGGTTTCTGATTTTGTGACTGATGTGGTTCGAGATTGTGTTTATAAGAGGGCAAACCTTACTGATTGGATTAAAAAAACTGATGCAGATGGGAAGAGTTCTTATTCGCTACTGATGTTGAGACTGGTCATTCTACTATGTTTGCTCACTGTGAACTTTGGGAAGTCTTTAGATATGCTCCTTGATGTTCTGGGGAAGAACTATGTCACTGCAGTACTGCCCAAGGAATTTGTTGATGTCCTTAAGAAGGGGAGGAAAGACAAATCTCCTACTGCCTGTGTGCTATCTATAGCTACTGCCCTTAAGGGGATTGGTAATCCTCTAGTCATTGCTAGTTTTGGGTCCAATTGTTCAAGGTTCTTATGCGCAGATGCCATTTATGTTGACATGTCATTAGACCTGTGCAAGGAGGACATTTTCAGGAAATTGTTCCCTAAATCCCCTGTTCTTCCAGCTTCAAAAAGCCAAATGAGGCCTGTTGAAGGGAAAGGTAATAATCGTTATACTTTTCTGTCCGATGATGGAGACACTGAGGGTGAAGATGAGGAGATTTGCATTTCCAATGACTCGAAGCCAAAGTGTCCAGAACCTGATGCTGCTTCTGCAACCCACGGGAAGAAAAAGGGCAACACCAAGAAGCCTAAGGGGAAAAAAGGCCGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2623

Amino Acids

298.49

Weight (kDa)

6.43

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 77 - 202 1.3e-58 Domain of unknown function (DUF6469)
AAA_11 PF13086 240 - 612 4.4e-36 AAA domain
AAA_19 PF13245 259 - 609 2.5e-07 AAA domain
AAA_12 PF13087 621 - 817 2.7e-56 AAA domain
UvrD-helicase PF00580 1316 - 1414 3.3e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3770, 7248
AasI GACNNNNNNGTC 1 cut(s) 6954
Acc16I TGCGCA 1 cut(s) 7570
Acc36I ACCTGC 2 cut(s) 1828, 4579
AccB1I GGYRCC 1 cut(s) 3348
AccB7I CCANNNNNTGG 4 cut(s) 1088, 1381, 2739, 3247
AccI GTMKAC 3 cut(s) 4087, 5194, 6897
AccIII TCCGGA 1 cut(s) 1594
AciI CCGC 7 cut(s) 2152, 2390, 3040, 5334, 6789, 6905, 7861
AcoI YGGCCR 5 cut(s) 45, 3017, 3815, 4824, 5076
AcuI CTGAAG 5 cut(s) 626, 963, 1467, 2816, 5199
AcvI CACGTG 2 cut(s) 188, 5039
AdeI CACNNNGTG 1 cut(s) 5085
AflII CTTAAG 2 cut(s) 7445, 7505
AflIII ACRYGT 6 cut(s) 185, 591, 1499, 3484, 4185, 7590
AhdI GACNNNNNGTC 2 cut(s) 1200, 3453
AjnI CCWGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
AleI CACNNNNGTG 2 cut(s) 446, 1238
AloI GAACNNNNNNTCC 4 cut(s) 3953, 3985, 5158, 5190
Alw21I GWGCWC 7 cut(s) 193, 229, 524, 4618, 4847, 4923, 7133
Alw44I GTGCAC 2 cut(s) 189, 7129
AlwNI CAGNNNCTG 5 cut(s) 2739, 3299, 3416, 5112, 7793
Ama87I CYCGRG 1 cut(s) 2987
Aor13HI TCCGGA 1 cut(s) 1594
ApaLI GTGCAC 2 cut(s) 189, 7129
ArsI GACNNNNNNTTYG 2 cut(s) 6965, 6997
AseI ATTAAT 2 cut(s) 2400, 2964
Asp700I GAANNNNTTC 6 cut(s) 117, 1545, 2576, 2669, 5399, 7631
AspA2I CCTAGG 1 cut(s) 4799
AspLEI GCGC 2 cut(s) 7089, 7571
AspS9I GGNCC 9 cut(s) 66, 738, 803, 1079, 2048, 2491, 4681, 6947, 7545
AvaI CYCGRG 1 cut(s) 2987
AvaII GGWCC 7 cut(s) 66, 738, 803, 2048, 2491, 6947, 7545
AvrII CCTAGG 1 cut(s) 4799
AxyI CCTNAGG 1 cut(s) 7845
BaeGI GKGCMC 4 cut(s) 193, 632, 889, 7133
BaeI ACNNNNGTAYC 2 cut(s) 4369, 4402
BalI TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
BanI GGYRCC 1 cut(s) 3348
BanII GRGCYC 1 cut(s) 4923
BarI GAAGNNNNNNTAC 4 cut(s) 1426, 1458, 4062, 4094
BauI CACGAG 1 cut(s) 4882
BbrPI CACGTG 2 cut(s) 188, 5039
BbsI GAAGAC 9 cut(s) 49, 1199, 1464, 1491, 2300, 2541, 3856, 5867, 7034
Bbv12I GWGCWC 7 cut(s) 193, 229, 524, 4618, 4847, 4923, 7133
BcgI CGANNNNNNTGC 2 cut(s) 5228, 5262
BciT130I CCWGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 2 cut(s) 3196, 6834
BfoI RGCGCY 1 cut(s) 7090
BfrI CTTAAG 2 cut(s) 7445, 7505
BfuAI ACCTGC 2 cut(s) 1828, 4579
BfuI GTATCC 1 cut(s) 177
BlnI CCTAGG 1 cut(s) 4799
BmcAI AGTACT 2 cut(s) 5814, 7419
Bme1390I CCNGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
Bme18I GGWCC 7 cut(s) 66, 738, 803, 2048, 2491, 6947, 7545
BmeRI GACNNNNNGTC 2 cut(s) 1200, 3453
BmeT110I CYCGRG 1 cut(s) 2987
BmgT120I GGNCC 9 cut(s) 66, 738, 803, 1079, 2048, 2491, 4681, 6947, 7545
BmiI GGNNCC 9 cut(s) 68, 739, 804, 1188, 2309, 3350, 3612, 6948, 7546
BmrFI CCNGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
BmrI ACTGGG 3 cut(s) 744, 3309, 5122
BmuI ACTGGG 3 cut(s) 744, 3309, 5122
BoxI GACNNNNGTC 2 cut(s) 878, 1809
BpiI GAAGAC 9 cut(s) 49, 1199, 1464, 1491, 2300, 2541, 3856, 5867, 7034
BpmI CTGGAG 7 cut(s) 1289, 2761, 4325, 4485, 4539, 5345, 7054
Bpu10I CCTNAGC 2 cut(s) 1075, 5388
BpuEI CTTGAG 4 cut(s) 2208, 2393, 4388, 4706
BsaAI YACGTR 4 cut(s) 188, 4867, 5039, 6678
BsaBI GATNNNNATC 2 cut(s) 3508, 6072
BsaI GGTCTC 1 cut(s) 2737
BsaWI WCCGGW 1 cut(s) 1594
BsaXI ACNNNNNCTCC 6 cut(s) 611, 641, 5018, 5048, 6946, 6976
Bse21I CCTNAGG 1 cut(s) 7845
Bse3DI GCAATG 7 cut(s) 1099, 1701, 3641, 5007, 5718, 5792, 7530
Bse8I GATNNNNATC 2 cut(s) 3508, 6072
BseAI TCCGGA 1 cut(s) 1594
BseBI CCWGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
BseJI GATNNNNATC 2 cut(s) 3508, 6072
BseMI GCAATG 7 cut(s) 1099, 1701, 3641, 5007, 5718, 5792, 7530
BseRI GAGGAG 3 cut(s) 5039, 5381, 7763
BseSI GKGCMC 4 cut(s) 193, 632, 889, 7133
BseYI CCCAGC 1 cut(s) 2908
BsgI GTGCAG 3 cut(s) 1940, 3043, 4215
BshNI GGYRCC 1 cut(s) 3348
BsiHKAI GWGCWC 7 cut(s) 193, 229, 524, 4618, 4847, 4923, 7133
BsiHKCI CYCGRG 1 cut(s) 2987
BsiSI CCGG 1 cut(s) 1595
BslFI GGGAC 4 cut(s) 52, 751, 2897, 6960
BsmBI CGTCTC 2 cut(s) 98, 1058
BsmFI GGGAC 4 cut(s) 52, 751, 2897, 6960
BsmI GAATGC 3 cut(s) 766, 1547, 6136
Bso31I GGTCTC 1 cut(s) 2737
BsoBI CYCGRG 1 cut(s) 2987
Bsp1286I GDGCHC 9 cut(s) 193, 229, 524, 632, 889, 4618, 4847, 4923, 7133
Bsp13I TCCGGA 1 cut(s) 1594
Bsp1407I TGTACA 2 cut(s) 2160, 6130
Bsp19I CCATGG 2 cut(s) 325, 6908
BspACI CCGC 7 cut(s) 2152, 2390, 3040, 5334, 6789, 6905, 7861
BspEI TCCGGA 1 cut(s) 1594
BspHI TCATGA 6 cut(s) 772, 3199, 3604, 5056, 6831, 7045
BspLI GGNNCC 9 cut(s) 68, 739, 804, 1188, 2309, 3350, 3612, 6948, 7546
BspMAI CTGCAG 3 cut(s) 3412, 5377, 7417
BspMI ACCTGC 2 cut(s) 1828, 4579
BspQI GCTCTTC 3 cut(s) 1826, 2620, 4911
BspT107I GGYRCC 1 cut(s) 3348
BspTI CTTAAG 2 cut(s) 7445, 7505
BspTNI GGTCTC 1 cut(s) 2737
BsrDI GCAATG 7 cut(s) 1099, 1701, 3641, 5007, 5718, 5792, 7530
BsrGI TGTACA 2 cut(s) 2160, 6130
BssNAI GTATAC 1 cut(s) 5195
BssSI CACGAG 1 cut(s) 4882
BssT1I CCWWGG 8 cut(s) 325, 2487, 2550, 4799, 5761, 6908, 6950, 7425
Bst1107I GTATAC 1 cut(s) 5195
Bst2BI CACGAG 1 cut(s) 4882
Bst2UI CCWGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
BstAFI CTTAAG 2 cut(s) 7445, 7505
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 2 cut(s) 2160, 6130
BstBAI YACGTR 4 cut(s) 188, 4867, 5039, 6678
BstDSI CCRYGG 3 cut(s) 325, 6908, 7813
BstENI CCTNNNNNAGG 4 cut(s) 5765, 7448, 7607, 7683
BstH2I RGCGCY 1 cut(s) 7090
BstHHI GCGC 2 cut(s) 7089, 7571
BstMWI GCNNNNNNNGC 9 cut(s) 233, 1448, 1859, 3911, 4972, 5243, 5720, 5847, 7805
BstNI CCWGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
BstPAI GACNNNNGTC 2 cut(s) 878, 1809
BstSCI CCNGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
BstSLI GKGCMC 4 cut(s) 193, 632, 889, 7133
BstV2I GAAGAC 9 cut(s) 49, 1199, 1464, 1491, 2300, 2541, 3856, 5867, 7034
BstX2I RGATCY 4 cut(s) 3049, 3217, 4150, 6213
BstXI CCANNNNNNTGG 4 cut(s) 43, 3015, 5028, 6395
BstYI RGATCY 4 cut(s) 3049, 3217, 4150, 6213
BstZ17I GTATAC 1 cut(s) 5195
Bsu36I CCTNAGG 1 cut(s) 7845
BsuI GTATCC 1 cut(s) 177
BtgI CCRYGG 3 cut(s) 325, 6908, 7813
BtsI GCAGTG 4 cut(s) 1659, 2425, 5493, 7410
BveI ACCTGC 2 cut(s) 1828, 4579
CaiI CAGNNNCTG 5 cut(s) 2739, 3299, 3416, 5112, 7793
CciI TCATGA 6 cut(s) 772, 3199, 3604, 5056, 6831, 7045
CfoI GCGC 2 cut(s) 7089, 7571
Cfr13I GGNCC 9 cut(s) 66, 738, 803, 1079, 2048, 2491, 4681, 6947, 7545
CseI GACGC 1 cut(s) 2112
CsiI ACCWGGT 1 cut(s) 1774
CspCI CAANNNNNGTGG 4 cut(s) 3526, 3561, 4989, 5024
DraI TTTAAA 4 cut(s) 484, 4180, 4467, 4632
DraIII CACNNNGTG 1 cut(s) 5085
DrdI GACNNNNNNGTC 1 cut(s) 6954
DriI GACNNNNNGTC 2 cut(s) 1200, 3453
DseDI GACNNNNNNGTC 1 cut(s) 6954
EaeI YGGCCR 5 cut(s) 45, 3017, 3815, 4824, 5076
Eam1105I GACNNNNNGTC 2 cut(s) 1200, 3453
Ecl136II GAGCTC 1 cut(s) 4921
Eco130I CCWWGG 8 cut(s) 325, 2487, 2550, 4799, 5761, 6908, 6950, 7425
Eco147I AGGCCT 3 cut(s) 2486, 5789, 7678
Eco24I GRGCYC 1 cut(s) 4923
Eco31I GGTCTC 1 cut(s) 2737
Eco32I GATATC 3 cut(s) 2905, 4009, 5959
Eco47I GGWCC 7 cut(s) 66, 738, 803, 2048, 2491, 6947, 7545
Eco53kI GAGCTC 1 cut(s) 4921
Eco57I CTGAAG 5 cut(s) 626, 963, 1467, 2816, 5199
Eco72I CACGTG 2 cut(s) 188, 5039
Eco81I CCTNAGG 1 cut(s) 7845
Eco88I CYCGRG 1 cut(s) 2987
EcoICRI GAGCTC 1 cut(s) 4921
EcoNI CCTNNNNNAGG 4 cut(s) 5765, 7448, 7607, 7683
EcoO109I RGGNCCY 3 cut(s) 803, 4681, 6947
EcoRI GAATTC 3 cut(s) 3118, 6196, 6703
EcoRII CCWGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
EcoRV GATATC 3 cut(s) 2905, 4009, 5959
EcoT14I CCWWGG 8 cut(s) 325, 2487, 2550, 4799, 5761, 6908, 6950, 7425
EcoT22I ATGCAT 2 cut(s) 1935, 2757
EcoT38I GRGCYC 1 cut(s) 4923
ErhI CCWWGG 8 cut(s) 325, 2487, 2550, 4799, 5761, 6908, 6950, 7425
Esp3I CGTCTC 2 cut(s) 98, 1058
FalI AAGNNNNNCTT 2 cut(s) 5381, 5413
FaqI GGGAC 4 cut(s) 52, 751, 2897, 6960
FauI CCCGC 2 cut(s) 6782, 6912
FauNDI CATATG 5 cut(s) 232, 268, 2215, 5160, 6024
FbaI TGATCA 2 cut(s) 3196, 6834
FblI GTMKAC 3 cut(s) 4087, 5194, 6897
FriOI GRGCYC 1 cut(s) 4923
FspI TGCGCA 1 cut(s) 7570
GlaI GCGC 2 cut(s) 7088, 7570
GsaI CCCAGC 1 cut(s) 2912
GsuI CTGGAG 7 cut(s) 1289, 2761, 4325, 4485, 4539, 5345, 7054
HaeII RGCGCY 1 cut(s) 7090
HapII CCGG 1 cut(s) 1595
HgaI GACGC 1 cut(s) 2112
HhaI GCGC 2 cut(s) 7089, 7571
Hin6I GCGC 2 cut(s) 7087, 7569
HinP1I GCGC 2 cut(s) 7087, 7569
HincII GTYRAC 7 cut(s) 1396, 2302, 2363, 3837, 4088, 6898, 7588
HindII GTYRAC 7 cut(s) 1396, 2302, 2363, 3837, 4088, 6898, 7588
HindIII AAGCTT 8 cut(s) 6, 582, 1867, 2726, 3845, 5610, 6009, 6494
HpaI GTTAAC 1 cut(s) 3837
HpaII CCGG 1 cut(s) 1595
Hpy99I CGWCG 1 cut(s) 4089
HpyCH4IV ACGT 8 cut(s) 78, 91, 187, 1026, 4866, 5038, 5864, 6677
HpyF10VI GCNNNNNNNGC 9 cut(s) 233, 1448, 1859, 3911, 4972, 5243, 5720, 5847, 7805
HpySE526I ACGT 8 cut(s) 78, 91, 187, 1026, 4866, 5038, 5864, 6677
HspAI GCGC 2 cut(s) 7087, 7569
Kpn2I TCCGGA 1 cut(s) 1594
Ksp22I TGATCA 2 cut(s) 3196, 6834
KspAI GTTAAC 1 cut(s) 3837
LguI GCTCTTC 3 cut(s) 1826, 2620, 4911
LmnI GCTCC 8 cut(s) 334, 1003, 1996, 3902, 4504, 4580, 5533, 7386
MabI ACCWGGT 1 cut(s) 1774
MaeII ACGT 8 cut(s) 78, 91, 187, 1026, 4866, 5038, 5864, 6677
MfeI CAATTG 5 cut(s) 1736, 2356, 4239, 4665, 7549
MflI RGATCY 4 cut(s) 3049, 3217, 4150, 6213
MhlI GDGCHC 9 cut(s) 193, 229, 524, 632, 889, 4618, 4847, 4923, 7133
MlsI TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
MluNI TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
MmeI TCCRAC 8 cut(s) 1073, 2090, 3265, 4552, 5634, 5661, 6559, 6624
Mox20I TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
Mph1103I ATGCAT 2 cut(s) 1935, 2757
MroI TCCGGA 1 cut(s) 1594
MroXI GAANNNNTTC 6 cut(s) 117, 1545, 2576, 2669, 5399, 7631
MscI TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
MslI CAYNNNNRTG 5 cut(s) 446, 771, 1238, 5498, 6881
Msp20I TGGCCA 4 cut(s) 3019, 3817, 4826, 5078
MspA1I CMGCKG 1 cut(s) 6789
MspCI CTTAAG 2 cut(s) 7445, 7505
MspI CCGG 1 cut(s) 1595
MspR9I CCNGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
MssI GTTTAAAC 1 cut(s) 4180
MunI CAATTG 5 cut(s) 1736, 2356, 4239, 4665, 7549
Mva1269I GAATGC 3 cut(s) 766, 1547, 6136
MvaI CCWGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
MwoI GCNNNNNNNGC 9 cut(s) 233, 1448, 1859, 3911, 4972, 5243, 5720, 5847, 7805
NcoI CCATGG 2 cut(s) 325, 6908
NdeI CATATG 5 cut(s) 232, 268, 2215, 5160, 6024
NlaIV GGNNCC 9 cut(s) 68, 739, 804, 1188, 2309, 3350, 3612, 6948, 7546
NmeAIII GCCGAG 1 cut(s) 5845
NsbI TGCGCA 1 cut(s) 7570
NsiI ATGCAT 2 cut(s) 1935, 2757
OliI CACNNNNGTG 2 cut(s) 446, 1238
PaeI GCATGC 4 cut(s) 1790, 4652, 5483, 6880
PaeR7I CTCGAG 1 cut(s) 2987
PagI TCATGA 6 cut(s) 772, 3199, 3604, 5056, 6831, 7045
PceI AGGCCT 3 cut(s) 2486, 5789, 7678
PciI ACATGT 5 cut(s) 591, 1499, 3484, 4185, 7590
PciSI GCTCTTC 3 cut(s) 1826, 2620, 4911
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 3 cut(s) 766, 1547, 6136
PdmI GAANNNNTTC 6 cut(s) 117, 1545, 2576, 2669, 5399, 7631
PflFI GACNNNGTC 1 cut(s) 2106
PflMI CCANNNNNTGG 4 cut(s) 1088, 1381, 2739, 3247
PfoI TCCNGGA 1 cut(s) 1983
PmaCI CACGTG 2 cut(s) 188, 5039
PmeI GTTTAAAC 1 cut(s) 4180
PmlI CACGTG 2 cut(s) 188, 5039
Ppu21I YACGTR 4 cut(s) 188, 4867, 5039, 6678
PpuMI RGGWCCY 2 cut(s) 803, 6947
PscI ACATGT 5 cut(s) 591, 1499, 3484, 4185, 7590
PshAI GACNNNNGTC 2 cut(s) 878, 1809
PshBI ATTAAT 2 cut(s) 2400, 2964
PsiI TTATAA 2 cut(s) 3770, 7248
Psp124BI GAGCTC 1 cut(s) 4923
Psp5II RGGWCCY 2 cut(s) 803, 6947
Psp6I CCWGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
PspCI CACGTG 2 cut(s) 188, 5039
PspFI CCCAGC 1 cut(s) 2908
PspGI CCWGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
PspN4I GGNNCC 9 cut(s) 68, 739, 804, 1188, 2309, 3350, 3612, 6948, 7546
PspPI GGNCC 9 cut(s) 66, 738, 803, 1079, 2048, 2491, 4681, 6947, 7545
PspPPI RGGWCCY 2 cut(s) 803, 6947
PsrI GAACNNNNNNTAC 2 cut(s) 4764, 4796
PstI CTGCAG 3 cut(s) 3412, 5377, 7417
PstNI CAGNNNCTG 5 cut(s) 2739, 3299, 3416, 5112, 7793
PsuI RGATCY 4 cut(s) 3049, 3217, 4150, 6213
PsyI GACNNNGTC 1 cut(s) 2106
RseI CAYNNNNRTG 5 cut(s) 446, 771, 1238, 5498, 6881
SacI GAGCTC 1 cut(s) 4923
SalI GTCGAC 2 cut(s) 4086, 6896
SapI GCTCTTC 3 cut(s) 1826, 2620, 4911
Sau96I GGNCC 9 cut(s) 66, 738, 803, 1079, 2048, 2491, 4681, 6947, 7545
ScaI AGTACT 2 cut(s) 5814, 7419
ScrFI CCNGG 9 cut(s) 354, 810, 1776, 1985, 2015, 2312, 2927, 3899, 7062
SduI GDGCHC 9 cut(s) 193, 229, 524, 632, 889, 4618, 4847, 4923, 7133
SexAI ACCWGGT 1 cut(s) 1774
Sfr274I CTCGAG 1 cut(s) 2987
SinI GGWCC 7 cut(s) 66, 738, 803, 2048, 2491, 6947, 7545
SlaI CTCGAG 1 cut(s) 2987
SmiMI CAYNNNNRTG 5 cut(s) 446, 771, 1238, 5498, 6881
SmlI CTYRAG 7 cut(s) 2223, 2372, 2987, 4403, 4685, 7445, 7505
SmoI CTYRAG 7 cut(s) 2223, 2372, 2987, 4403, 4685, 7445, 7505
SphI GCATGC 4 cut(s) 1790, 4652, 5483, 6880
SseBI AGGCCT 3 cut(s) 2486, 5789, 7678
SsiI CCGC 7 cut(s) 2152, 2390, 3040, 5334, 6789, 6905, 7861
SstI GAGCTC 1 cut(s) 4923
StuI AGGCCT 3 cut(s) 2486, 5789, 7678
StyD4I CCNGG 9 cut(s) 352, 808, 1774, 1983, 2013, 2310, 2925, 3897, 7060
StyI CCWWGG 8 cut(s) 325, 2487, 2550, 4799, 5761, 6908, 6950, 7425
TaiI ACGT 8 cut(s) 81, 94, 190, 1029, 4869, 5041, 5867, 6680
TaqII GACCGA 1 cut(s) 2761
TatI WGTACW 6 cut(s) 1923, 2160, 2614, 5812, 6130, 7417
TauI GCSGC 2 cut(s) 5336, 7863
TspGWI ACGGA 5 cut(s) 1182, 1351, 3884, 4373, 5532
Tth111I GACNNNGTC 1 cut(s) 2106
Van91I CCANNNNNTGG 4 cut(s) 1088, 1381, 2739, 3247
Vha464I CTTAAG 2 cut(s) 7445, 7505
VneI GTGCAC 2 cut(s) 189, 7129
VpaK11BI GGWCC 7 cut(s) 66, 738, 803, 2048, 2491, 6947, 7545
VspI ATTAAT 2 cut(s) 2400, 2964
XagI CCTNNNNNAGG 4 cut(s) 5765, 7448, 7607, 7683
XcmI CCANNNNNNNNNTGG 1 cut(s) 1089
XhoI CTCGAG 1 cut(s) 2987
XmaJI CCTAGG 1 cut(s) 4799
XmiI GTMKAC 3 cut(s) 4087, 5194, 6897
XmnI GAANNNNTTC 6 cut(s) 117, 1545, 2576, 2669, 5399, 7631
ZrmI AGTACT 2 cut(s) 5814, 7419
Zsp2I ATGCAT 2 cut(s) 1935, 2757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.