Rh4DG435800

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000223
Physical Location & Seq
Forward (+)
63919778 .. 63927609
7832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG435800.1

Sequence Viewer

Length: 5832 bp
ATGATGGAAGGTTCAGAAAGCTTCACTGACACTGTGCTTTCTTGGTCCCTTGAAGACATATGTAATGAAAACCTTTACAAGCACCAGGTGGAAAAGATTCCCGAGTCAGTTCAACAATTTGGGGCATGTTCATATCCATTATTGGATGAAACTCGAGCTCAGTTGCAATCCAGTATGGAATCCATGCACAGAGCGCCATTTTGTCAAGTAAATGCCATCCAAGAGTCAAAGCACTATGAAGGAGAAACAAAGCTATATGATATAGAGATTGATGGCTGGAGAAACAGTGATTCTGGCAAGGAGTTGCACAAACCCATTCAGGGAGATGTTTTTGTTTTAGCAGATGCTAAACTCGAAACTATATCTGATTTACGAAAGTTGGGGAAATGGTGGTCACTTGTAATAGTCACTGAGGTCTCCACAGATAAGAAAGAGGATGATAGAACCTTTCTTTCCCTTAAAGTCAGAGCACCGAAAGAATTTGAAGTCAACAATGGCAATGGAACATCACTGTTTTTGGTCTTCTTAGCAAACATAACTCCTAACTTAAGGATATGGAACGCAATGCACATGTGCATCAGTTCCAGATGGAAGGTGCTGTTTGGTGATAACTTTATGAAATCATTCAAAAAGTTGAAGTCATTCCAAAAGAAGATGTCAGTACTGAATCTTCTTCTCAAGCTTTCGAGTGGCTGGAGACCCAAGAAAAGAAATGTAGACACAATATGTAAAATCTCTTCGATGACACTGAAGCAATTTAAGGTCGATGATCTATATATTGTTTTGACAATCGACATTGAAAAGGATTTGAAATACATGCAAGTGTTGAGGATGTGGGACTTATTGCCTGGTCTGTTGGATATCCAAAAATTGGCAGATCGATTGGACTGCATATTTAGTAGATACACGAATCTTTTTGTCAATCTCTGTAGAGAGAAATGTCTTGAGGGTCATTTGGAAGTTCCAGCAAGCTGGCCCCTCTCTTTGGATGTTGTCCGCTATAAAGATCTAAGCAGCACCAAAAGCATGAGTAATTCTGTTGGTGACTCTTCTGTGAATGGAAGTTGTGTTGAGAATTCAAAGGTCAATGAGAGCTTGTTGCTGATGAAGTTTTACTCCTTATCATCTGGGGTAGTGAACTTCTTGTTATCTGACAGAGAGGACAGCAAAGTGGATCTCCCTTTTGAAGTTACTGACCAAGAAAGGGAGGCTGTTCTTTATGATAGAAGTACATTTATACTGGGACGGTCAGGCACTGGAAAGACAACAGTTTTGACTACAAAGTTATATCAGAAAGAACAGCAGCACCTTATGGTTGATGAAGGTTTCTATGATGTTGAAAGCAATGCAGTCGGGCATGCTGGTCTGCATAATAAAGCTGTACAGAGTTCTTCTTCTGCATTAAATAAAGGGATGGTGTTACACCAGCTTTTTGTGACCGTGAGTCCTAAACTCTGTTTTGCCGTCAAACAACATTTTTTACGCTTGAAAAGTTTTGCTTCCGGCAGAAGCAGTTTGGTTGATATGGATGATTTTGATGATGAGGAAGCTCAATTTAGGGGTATCCCAGATTCTTTTTGTGATATTCCTCCAAAATTTTATCCGCTTTTCGTAACATTTCATAAGTTCTTGATGATGCTTGATGGAAGTCTGAGTAATTCATACTTTGAAAGATTCCCAGACATCGCAAAACTACCTCAAGATCGACAGCGAATATCAAGAGCTAATGTGCTGCAGACCTTCCTCACCACCAAGGAGGTCACATATGCAAGGTTTAGCTCATCATATTGGCCTCATTTTGATGTTCAGTTGACAAAGAAGCTTGATGCTTCACGAGTCTTTACTGAGATTATTTCTCATATCAAAGGTGGTCTTAGAGCGTTGGAAGCAGGTGATGGAAAACTCAGTCAGTTGGATTATGTGCAAATGTCAGAGGGCCGGGCTTCCAGTTTAAGCCAGCAAAAGAGAGAGATAATATATGATATATTTCAGGTATATGAGAAAATGAAAATGAGAAATGGTGAGTTTGATATTGCTGATTTTGTAAATGATATTCACCATCGGCTCAAATGTGAAAAATACAAGGGTGATGAAATTGATTTTGTGTACATTGATGAGGTCCAGGATCTTGCCATGAGCCAAATTGCATTGTTTAAACATATCTGCAGCAATGTTGAAGAGGGTTTCGTTTTTTCTGGAGATACAGCCCAGACTATAGCAAGGGGTATTGATTTTAGATTCCAAGATATACGACATCTCTTTTACAAGAAGTTTGTATTGGAATCAAGAAAAAAAAAACACGACAATAGGAAAGAAAAAGGACAGATCTCAAACATCTTTCAGTTGACACAAAACTTCCGAAGCCATGATGGGATACTGAAGTTGTTGCAGAGCATTGTTGAACTGATTTATCATTTTTTCCCCCTCTGTATTGATGTCTTAGAACCAGAAACGAGTATGATATATGGGGAAGCTCCGATTCTACTCTATTCTGGAAATGATGAAAAACTATTCAAAAAAATTCTAGGAAGTAGTGGGATTATTGCCGGAAATATGGGTGGCTTTGGTGCGGATCAAGTTATTTTGGTCCGTGATGATTGTTCTCGAGAAGAAATCTCTAACTCCATTGGGAAGCAAGCTCTAGTTCTTACTATAGTGGAGTGCAAGGGTCTAGAATTTGAGGATGTACTCCTATACAAATTTTTTGGATCATCACCACTGAAAAATCAATGGAGGGTGCTATATGAATTCATGCAAGAACAAAATTTGCTGGACTCCACATTACCTGAGCGCTTTCCAAGTTTTGATGATGCCAAACACAGCATGTTATGCTCTGAACTAAAGCAGTTATATGTGGCTGTCTCTCGTACAAGACAGAGACTCTGGATATACGAGAATGTTGAAGAACTTTCCAACCCAATTTTTGACTATTGGAAAAGGCAACATCTTGTACAACTTAGAGAACTAGACGATTCACTTGCACAAATTATGCAAGTTGCAAGCACTTCAGGAGACTGGAGATCACGTGGCATCAAGCTATATCATGAGCATAACTACAAAATGGCCAGAATGTGCTTTCATAAAGCTGGTGATACATTTTGGGAAAGACGGTCTGAAGCTGCTGAGCTTAAAGTCAAGGCAGACCATATGCGCACTTCTAGTCATGGAGAGGCAAATGCACTCCTTAGGAAAGCAGCTTCAATTTTTGAAGCTATAGGCCTGTTTGTACCTTCTGCCAGATGCTTTTATGATTTGGGCGAGTATGAACGAGCAGGAACAATTTATTTGTATAAATGTGGCGAACTGGAACTGGAAAATGCTGGGGAGTGCTTTTATCTTGCTGAAAATTATGTGCTTGCTGCAGATGTGTATGCTAGAGGAAACTTTTTCTTTCTGAGTATTTCTTCTTGTTTACGAGGAAATATATTTCACAGAGGTTTGGAATACATAAAATATTGGAGACAACATGCAAGAGACGAGTATGATCTGGCCAGACAAGCAGATGAGACTCATGAAATGGAATTGGAATTTCTTGAGCATTGCGCATTTCACTATTATGAGGGAAAAGATGACAGATCAAGGATGAAAGTTCTTAGAGATGGAATGCTTTCTACTCGAAAGTTTTTGAATGCTCAGATTTCATCACGCACTTCAAAATATTTGTGGGAAGAAAAACTGCCTGGTGATCTTAAAAGGCCTTCAAACAAAAAATATGAGAAGCAGGTTTCTATTGATTCACTGGTCTACTTCTGGAATTCTTGGAAGGATACAGTTGTACACCTGTTTGAATATCTTGGATCGATAGATGTTAATGATTACATAAGTCATGGTGACTTCTTTCTGAATTATTTAGGGGTGTGGAGGCATTTTCATGATGGTCTAAATCCGCTCTATCTTTCACTCATTTATGATATTGACTGGGTGAGAAGTATTGACAAGAGATCTTTCCCAAGAAATGAGGAGTCAATCCCCATTGATGTTCACCGACTTGTCTTGGATGCTCAGAGTTATTGGAGTTCAGAGATGCTTTCTCTTGGCATTAAGGTTTTGGAGAAGCTTGAAGTGCTTTACAACTTCGCAATCAATAATTCTGATTCAGTGTTCTGCCAGAGCCGGTCTCTTATCCTTATATACGAGGTTGCAAAATATCTTCTGGAATCCAAATTTCTGAAGATGACTCATTATCATGCTGAGACACTCCACAGATTTGTTACATTGTCGACTGAGAATTTTGTCTCTTACATATTTCCTTTAGATTGGATGAGGTCATCAAGAGAGGATATGATTTCTCTTAGGCAAATTGATTCTTGTAAGTGTTTACTGAATCAAGTCATAGTTGAGTACATCAGCTCGACAAACAGGCTTTCATCTGGGCAAATGGGATGTATTGCGATGATCATTCTTGGGTCCGGTAAGCTGGATAATGAACTTTATGAGAAGCTTATAAAAAATCTAGACTGCAACTCTCCATGGAAAAAGTTCATTGAGGATCTATGTGACAGTATTCAGTCAGAGCTGCCAGAAGGTTATAATAGTGAGCGCCCTAAAAATGCCACAGTTCTTGAATATTCGGTGGCACCTGAGAGAGGTCCTTTACTTGGAAATATCAGTCATCAAGAACCGAGAGAGGTGTCTCTTTTGTGGAAGTTCCATGGTGCTTTGTTCAAAACTTATAGTCAGAATTGGAGTTTACATTGCTACAATATAACACCTGATTGTTTCTTATATCTTGTAGATTGCCTTCTGATTCGGATCTGTTGCTTTCAAGGTTATGCAATCACTACAAGATCTTGTTTCATTGAGCGGCTGATATATAAGGAGGAACATAATCAAGTGATAGTGGATGATGTTCGAATATCTTTTGAATGCATCCTTCAGTTTCTGACTGATGTTGTTCGGGAATTTCTTTTTAATAAGACGGATATGATTAAGTGGATAAAAAAGTGTACCAATAATAGTTGGAAGGTGTATTACTCGCTACTGATGCAGAGATTGGTTTTTGTGTTATGTCTGCTTTATTTGAATTTTGGGATGGGCTTTGATATTCTCTTGGATTTGCTGAGGAGGAAGTACATCACTGACCAGTTACCAAAGGAGTTCTGCTATGTCCTTAGGGGAATTGCATCTTTACGTGATTCTGTTAGTAGACATGTTGATGTGCTAGCTCGAGCTTTTAAGAAGAGCGGTAATCCTTTGGTGGTTGCAAGTTTTGGGATTGATTGTTCAAGGTTCTTCTGTTCAGATGCCATTATCATCAACATGAAGGCTAACATATGCATGGCTGAAATAGTAAGATCTCTATTTCCAAAACACTCTATGATTCGTTCTTCACAAGGGCAGACATACACCACTGAGAACCTTCGGCATTGGAACAGAAATAACTTCCCCGTGGATTTTGATCCCTTGTGGGAAATATTTAAAGGTTTTAAACCAGCAAAGGAAAGAGATCAGTGCAATATATTTTCAGATGCCTCAAAAATCAAGTTGGATGTGGAGAAGATTATTCGGCTTTTGGCTGCCGCATGGTCTGGCCTTGATGACAAAGAAGAACGAAAGTTATCTAGAGAAGTGCTGAGCATGCTGGATGAATTGGTGCAACTCTATGCCGCATTAGATGGGAGCGAACGTGAAGTTGGAAACAATATGGCTACAGTTGCAGAACTTTCCAGGAGGATGCAGTGGAGAAGTCAAAGATTTTTTTGGCCGGAAAAAATTCAGCCCATCTTCACTGAACTGTATATGGAGCACAATAATAAACTAGCTAGGAAGGCACATAAGGCTGTTGCCAAGTCTGGCCACGGCAATGAGGCAGGAAACGGTGATCGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1943

Amino Acids

225.23

Weight (kDa)

6.46

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 66 - 192 6e-40 Domain of unknown function (DUF6469)
UvrD-helicase PF00580 650 - 739 1.7e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4445, 4530
AarI CACCTGC 1 cut(s) 1880
Acc16I TGCGCA 2 cut(s) 3152, 3544
Acc36I ACCTGC 2 cut(s) 1880, 3712
AccB1I GGYRCC 1 cut(s) 4576
AccB7I CCANNNNNTGG 1 cut(s) 871
AccBSI CCGCTC 3 cut(s) 3889, 4804, 5184
AccI GTMKAC 4 cut(s) 717, 3744, 4220, 5146
AciI CCGC 8 cut(s) 997, 1604, 2570, 3887, 4804, 5184, 5520, 5607
AclWI GGATC 8 cut(s) 1182, 2133, 2580, 2716, 3805, 4497, 4760, 5393
AcoI YGGCCR 4 cut(s) 3063, 3489, 5702, 5794
AcuI CTGAAG 6 cut(s) 770, 2399, 2991, 3135, 4190, 4859
AcvI CACGTG 1 cut(s) 3026
AdeI CACNNNGTG 1 cut(s) 88
AfeI AGCGCT 1 cut(s) 2792
AfiI CCNNNNNNNGG 6 cut(s) 320, 871, 985, 1204, 4586, 4598
AflII CTTAAG 1 cut(s) 547
AflIII ACRYGT 2 cut(s) 570, 5149
AhdI GACNNNNNGTC 1 cut(s) 1443
AjnI CCWGG 5 cut(s) 84, 847, 2121, 3679, 5666
AjuI GAANNNNNNNTTGG 4 cut(s) 2261, 2293, 5616, 5648
AloI GAACNNNNNNTCC 2 cut(s) 5206, 5238
Alw21I GWGCWC 3 cut(s) 160, 472, 5748
AlwI GGATC 8 cut(s) 1182, 2133, 2580, 2716, 3805, 4497, 4760, 5393
AlwNI CAGNNNCTG 2 cut(s) 1256, 4882
Ama87I CYCGRG 4 cut(s) 101, 153, 2604, 5166
Aor51HI AGCGCT 1 cut(s) 2792
ApeKI GCWGC 9 cut(s) 1014, 1303, 1732, 2166, 3119, 3194, 3359, 4516, 5516
ArsI GACNNNNNNTTYG 2 cut(s) 1255, 1287
Asp700I GAANNNNTTC 6 cut(s) 96, 623, 641, 3607, 4478, 5712
AspLEI GCGC 5 cut(s) 196, 2793, 3153, 3545, 4542
AspS9I GGNCC 7 cut(s) 45, 975, 1936, 2119, 2587, 4407, 4589
AsuC2I CCSGG 1 cut(s) 1940
AsuII TTCGAA 1 cut(s) 4852
AsuNHI GCTAGC 1 cut(s) 5161
AvaI CYCGRG 4 cut(s) 101, 153, 2604, 5166
AvaII GGWCC 5 cut(s) 45, 2119, 2587, 4407, 4589
AxyI CCTNAGG 2 cut(s) 3185, 5111
BaeI ACNNNNGTAYC 4 cut(s) 2366, 2399, 3759, 3792
BalI TGGCCA 3 cut(s) 3065, 3491, 5796
BanI GGYRCC 1 cut(s) 4576
BanII GRGCYC 1 cut(s) 160
BarI GAAGNNNNNNTAC 8 cut(s) 654, 686, 721, 753, 4725, 4757, 4955, 4987
BauI CACGAG 1 cut(s) 1833
BbrPI CACGTG 1 cut(s) 3026
BbsI GAAGAC 2 cut(s) 60, 514
Bbv12I GWGCWC 3 cut(s) 160, 472, 5748
BbvCI CCTCAGC 1 cut(s) 5060
BbvI GCAGC 9 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346, 4503, 5503
BceAI ACGGC 2 cut(s) 1448, 5815
BcgI CGANNNNNNTGC 6 cut(s) 870, 904, 1333, 1367, 2960, 2994
BciT130I CCWGG 5 cut(s) 86, 849, 2123, 3681, 5668
BciVI GTATCC 3 cut(s) 1574, 2367, 3760
BclI TGATCA 1 cut(s) 4395
BcnI CCSGG 1 cut(s) 1940
BfmI CTRYAG 8 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360, 5649
BfoI RGCGCY 3 cut(s) 197, 2794, 4543
BfrI CTTAAG 1 cut(s) 547
BfuAI ACCTGC 2 cut(s) 1880, 3712
BfuI GTATCC 3 cut(s) 1574, 2367, 3760
BglII AGATCT 5 cut(s) 1006, 2325, 3941, 4787, 5294
BlpI GCTNAGC 2 cut(s) 3123, 5573
BmcAI AGTACT 1 cut(s) 663
Bme1390I CCNGG 6 cut(s) 86, 849, 1940, 2123, 3681, 5668
Bme18I GGWCC 5 cut(s) 45, 2119, 2587, 4407, 4589
BmeRI GACNNNNNGTC 1 cut(s) 1443
BmeT110I CYCGRG 4 cut(s) 101, 153, 2604, 5166
BmgT120I GGNCC 7 cut(s) 45, 975, 1936, 2119, 2587, 4407, 4589
BmiI GGNNCC 4 cut(s) 47, 977, 4408, 4578
BmrFI CCNGG 6 cut(s) 86, 849, 1940, 2123, 3681, 5668
BmrI ACTGGG 2 cut(s) 1250, 3928
BmtI GCTAGC 1 cut(s) 5165
BmuI ACTGGG 2 cut(s) 1250, 3928
BpiI GAAGAC 2 cut(s) 60, 514
BplI GAGNNNNNCTC 2 cut(s) 4102, 4134
BpmI CTGGAG 4 cut(s) 298, 715, 2217, 3037
Bpu10I CCTNAGC 2 cut(s) 2787, 5060
Bpu1102I GCTNAGC 2 cut(s) 3123, 5573
Bpu14I TTCGAA 1 cut(s) 4852
BpuEI CTTGAG 4 cut(s) 662, 965, 1683, 3554
BpuMI CCSGG 1 cut(s) 1940
Bsa29I ATCGAT 2 cut(s) 880, 3800
BsaAI YACGTR 2 cut(s) 3026, 5132
BsaBI GATNNNNATC 2 cut(s) 4751, 5397
BsaI GGTCTC 3 cut(s) 421, 691, 4122
BsaJI CCNNGG 5 cut(s) 1752, 4469, 4651, 5388, 5797
BsaWI WCCGGW 1 cut(s) 4409
BsaXI ACNNNNNCTCC 6 cut(s) 271, 301, 3010, 3040, 5611, 5641
Bsc4I CCNNNNNNNGG 6 cut(s) 320, 871, 985, 1204, 4586, 4598
Bse118I RCCGGY 1 cut(s) 4113
Bse21I CCTNAGG 2 cut(s) 3185, 5111
Bse3DI GCAATG 7 cut(s) 505, 570, 1351, 2176, 3538, 4693, 5809
Bse8I GATNNNNATC 2 cut(s) 4751, 5397
BseBI CCWGG 5 cut(s) 86, 849, 2123, 3681, 5668
BseCI ATCGAT 2 cut(s) 880, 3800
BseDI CCNNGG 5 cut(s) 1752, 4469, 4651, 5388, 5797
BseJI GATNNNNATC 2 cut(s) 4751, 5397
BseLI CCNNNNNNNGG 6 cut(s) 320, 871, 985, 1204, 4586, 4598
BseMI GCAATG 7 cut(s) 505, 570, 1351, 2176, 3538, 4693, 5809
BseRI GAGGAG 2 cut(s) 3974, 5077
BseXI GCAGC 9 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346, 4503, 5503
BseYI CCCAGC 1 cut(s) 3320
BshNI GGYRCC 1 cut(s) 4576
BshVI ATCGAT 2 cut(s) 880, 3800
BsiHKAI GWGCWC 3 cut(s) 160, 472, 5748
BsiHKCI CYCGRG 4 cut(s) 101, 153, 2604, 5166
BsiSI CCGG 6 cut(s) 1503, 1939, 2547, 4114, 4410, 5705
BslFI GGGAC 3 cut(s) 31, 851, 1257
BslI CCNNNNNNNGG 6 cut(s) 320, 871, 985, 1204, 4586, 4598
BsmBI CGTCTC 1 cut(s) 3468
BsmFI GGGAC 3 cut(s) 31, 851, 1257
BsmI GAATGC 3 cut(s) 3609, 3634, 4871
Bso31I GGTCTC 3 cut(s) 421, 691, 4122
BsoBI CYCGRG 4 cut(s) 101, 153, 2604, 5166
Bsp119I TTCGAA 1 cut(s) 4852
Bsp1286I GDGCHC 3 cut(s) 160, 472, 5748
Bsp1407I TGTACA 4 cut(s) 1381, 2106, 2950, 3775
Bsp1720I GCTNAGC 2 cut(s) 3123, 5573
Bsp19I CCATGG 2 cut(s) 4469, 4651
BspACI CCGC 8 cut(s) 997, 1604, 2570, 3887, 4804, 5184, 5520, 5607
BspDI ATCGAT 2 cut(s) 880, 3800
BspHI TCATGA 3 cut(s) 3043, 3511, 3871
BspLI GGNNCC 4 cut(s) 47, 977, 4408, 4578
BspMAI CTGCAG 3 cut(s) 1737, 2168, 3364
BspMI ACCTGC 2 cut(s) 1880, 3712
BspOI GCTAGC 1 cut(s) 5165
BspPI GGATC 8 cut(s) 1182, 2133, 2580, 2716, 3805, 4497, 4760, 5393
BspQI GCTCTTC 1 cut(s) 5174
BspT104I TTCGAA 1 cut(s) 4852
BspT107I GGYRCC 1 cut(s) 4576
BspTI CTTAAG 1 cut(s) 547
BspTNI GGTCTC 3 cut(s) 421, 691, 4122
BsrBI CCGCTC 3 cut(s) 3889, 4804, 5184
BsrDI GCAATG 7 cut(s) 505, 570, 1351, 2176, 3538, 4693, 5809
BsrFI RCCGGY 1 cut(s) 4113
BsrGI TGTACA 4 cut(s) 1381, 2106, 2950, 3775
BssAI RCCGGY 1 cut(s) 4113
BssECI CCNNGG 5 cut(s) 1752, 4469, 4651, 5388, 5797
BssSI CACGAG 1 cut(s) 1833
BssT1I CCWWGG 3 cut(s) 1752, 4469, 4651
Bst2BI CACGAG 1 cut(s) 1833
Bst2UI CCWGG 5 cut(s) 86, 849, 2123, 3681, 5668
Bst6I CTCTTC 4 cut(s) 742, 1054, 2172, 5174
BstAFI CTTAAG 1 cut(s) 547
BstAPI GCANNNNNTGC 1 cut(s) 2829
BstAUI TGTACA 4 cut(s) 1381, 2106, 2950, 3775
BstBAI YACGTR 2 cut(s) 3026, 5132
BstBI TTCGAA 1 cut(s) 4852
BstC8I GCNNGC 9 cut(s) 970, 974, 1359, 1958, 2637, 3001, 3357, 5163, 5579
BstDSI CCRYGG 4 cut(s) 4469, 4651, 5388, 5797
BstENI CCTNNNNNAGG 1 cut(s) 4584
BstH2I RGCGCY 3 cut(s) 197, 2794, 4543
BstHHI GCGC 5 cut(s) 196, 2793, 3153, 3545, 4542
BstNI CCWGG 5 cut(s) 86, 849, 2123, 3681, 5668
BstNSI RCATGY 8 cut(s) 129, 574, 820, 1361, 2827, 3470, 5153, 5581
BstSCI CCNGG 6 cut(s) 84, 847, 1938, 2121, 3679, 5666
BstSFI CTRYAG 8 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360, 5649
BstV1I GCAGC 9 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346, 4503, 5503
BstV2I GAAGAC 2 cut(s) 60, 514
BstX2I RGATCY 9 cut(s) 1006, 1174, 2125, 2325, 3941, 4489, 4752, 4787, 5294
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 9 cut(s) 1006, 1174, 2125, 2325, 3941, 4489, 4752, 4787, 5294
Bsu15I ATCGAT 2 cut(s) 880, 3800
Bsu36I CCTNAGG 2 cut(s) 3185, 5111
BsuI GTATCC 3 cut(s) 1574, 2367, 3760
BsuTUI ATCGAT 2 cut(s) 880, 3800
BtgI CCRYGG 4 cut(s) 4469, 4651, 5388, 5797
BtgZI GCGATG 2 cut(s) 1669, 4406
BtsI GCAGTG 1 cut(s) 5684
BveI ACCTGC 2 cut(s) 1880, 3712
Cac8I GCNNGC 9 cut(s) 970, 974, 1359, 1958, 2637, 3001, 3357, 5163, 5579
CaiI CAGNNNCTG 2 cut(s) 1256, 4882
CciI TCATGA 3 cut(s) 3043, 3511, 3871
CfoI GCGC 5 cut(s) 196, 2793, 3153, 3545, 4542
Cfr10I RCCGGY 1 cut(s) 4113
Cfr13I GGNCC 7 cut(s) 45, 975, 1936, 2119, 2587, 4407, 4589
ClaI ATCGAT 2 cut(s) 880, 3800
CsiI ACCWGGT 1 cut(s) 84
CspCI CAANNNNNGTGG 2 cut(s) 4543, 4578
DraI TTTAAA 3 cut(s) 2155, 5419, 5428
DraIII CACNNNGTG 1 cut(s) 88
DriI GACNNNNNGTC 1 cut(s) 1443
EaeI YGGCCR 4 cut(s) 3063, 3489, 5702, 5794
Eam1104I CTCTTC 4 cut(s) 742, 1054, 2172, 5174
Eam1105I GACNNNNNGTC 1 cut(s) 1443
EarI CTCTTC 4 cut(s) 742, 1054, 2172, 5174
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 3 cut(s) 1752, 4469, 4651
Eco147I AGGCCT 2 cut(s) 3219, 3697
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 3 cut(s) 421, 691, 4122
Eco32I GATATC 1 cut(s) 862
Eco47I GGWCC 5 cut(s) 45, 2119, 2587, 4407, 4589
Eco47III AGCGCT 1 cut(s) 2792
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 6 cut(s) 770, 2399, 2991, 3135, 4190, 4859
Eco72I CACGTG 1 cut(s) 3026
Eco81I CCTNAGG 2 cut(s) 3185, 5111
Eco88I CYCGRG 4 cut(s) 101, 153, 2604, 5166
EcoICRI GAGCTC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 4584
EcoO109I RGGNCCY 1 cut(s) 4589
EcoRI GAATTC 3 cut(s) 1075, 2747, 3754
EcoRII CCWGG 5 cut(s) 84, 847, 2121, 3679, 5666
EcoRV GATATC 1 cut(s) 862
EcoT14I CCWWGG 3 cut(s) 1752, 4469, 4651
EcoT22I ATGCAT 2 cut(s) 4871, 5279
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 1752, 4469, 4651
Esp3I CGTCTC 1 cut(s) 3468
FalI AAGNNNNNCTT 4 cut(s) 1483, 1515, 1857, 1889
FaqI GGGAC 3 cut(s) 31, 851, 1257
FauNDI CATATG 4 cut(s) 59, 1765, 3147, 5273
FbaI TGATCA 1 cut(s) 4395
FblI GTMKAC 4 cut(s) 717, 3744, 4220, 5146
FriOI GRGCYC 1 cut(s) 160
FspAI RTGCGCAY 1 cut(s) 3152
FspI TGCGCA 2 cut(s) 3152, 3544
GlaI GCGC 5 cut(s) 195, 2792, 3152, 3544, 4541
GsaI CCCAGC 1 cut(s) 3324
GsuI CTGGAG 4 cut(s) 298, 715, 2217, 3037
HaeII RGCGCY 3 cut(s) 197, 2794, 4543
HapII CCGG 6 cut(s) 1503, 1939, 2547, 4114, 4410, 5705
HhaI GCGC 5 cut(s) 196, 2793, 3153, 3545, 4542
Hin6I GCGC 5 cut(s) 194, 2791, 3151, 3543, 4540
HinP1I GCGC 5 cut(s) 194, 2791, 3151, 3543, 4540
HincII GTYRAC 4 cut(s) 490, 1812, 2346, 4221
HindII GTYRAC 4 cut(s) 490, 1812, 2346, 4221
HindIII AAGCTT 5 cut(s) 19, 680, 1820, 4055, 4439
HpaII CCGG 6 cut(s) 1503, 1939, 2547, 4114, 4410, 5705
HpyCH4IV ACGT 3 cut(s) 3025, 5131, 5626
HpySE526I ACGT 3 cut(s) 3025, 5131, 5626
HspAI GCGC 5 cut(s) 194, 2791, 3151, 3543, 4540
Ksp22I TGATCA 1 cut(s) 4395
LguI GCTCTTC 1 cut(s) 5174
LmnI GCTCC 3 cut(s) 2479, 5619, 5743
Lsp1109I GCAGC 9 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346, 4503, 5503
MabI ACCWGGT 1 cut(s) 84
MaeII ACGT 3 cut(s) 3025, 5131, 5626
MbiI CCGCTC 3 cut(s) 3889, 4804, 5184
MflI RGATCY 9 cut(s) 1006, 1174, 2125, 2325, 3941, 4489, 4752, 4787, 5294
MhlI GDGCHC 3 cut(s) 160, 472, 5748
MlsI TGGCCA 3 cut(s) 3065, 3491, 5796
MluNI TGGCCA 3 cut(s) 3065, 3491, 5796
MmeI TCCRAC 7 cut(s) 837, 1863, 1893, 2937, 4940, 5466, 5614
Mox20I TGGCCA 3 cut(s) 3065, 3491, 5796
Mph1103I ATGCAT 2 cut(s) 4871, 5279
MroXI GAANNNNTTC 6 cut(s) 96, 623, 641, 3607, 4478, 5712
MscI TGGCCA 3 cut(s) 3065, 3491, 5796
MslI CAYNNNNRTG 9 cut(s) 821, 1800, 3555, 3870, 4185, 5154, 5258, 5276, 5802
Msp20I TGGCCA 3 cut(s) 3065, 3491, 5796
MspCI CTTAAG 1 cut(s) 547
MspI CCGG 6 cut(s) 1503, 1939, 2547, 4114, 4410, 5705
MspR9I CCNGG 6 cut(s) 86, 849, 1940, 2123, 3681, 5668
MssI GTTTAAAC 1 cut(s) 2155
Mva1269I GAATGC 3 cut(s) 3609, 3634, 4871
MvaI CCWGG 5 cut(s) 86, 849, 2123, 3681, 5668
NciI CCSGG 1 cut(s) 1940
NcoI CCATGG 2 cut(s) 4469, 4651
NdeI CATATG 4 cut(s) 59, 1765, 3147, 5273
NheI GCTAGC 1 cut(s) 5161
NlaIV GGNNCC 4 cut(s) 47, 977, 4408, 4578
NmuCI GTSAC 7 cut(s) 393, 406, 1043, 1435, 1759, 3830, 4496
NsbI TGCGCA 2 cut(s) 3152, 3544
NsiI ATGCAT 2 cut(s) 4871, 5279
NspI RCATGY 8 cut(s) 129, 574, 820, 1361, 2827, 3470, 5153, 5581
NspV TTCGAA 1 cut(s) 4852
PaeI GCATGC 2 cut(s) 1361, 5581
PaeR7I CTCGAG 3 cut(s) 153, 2604, 5166
PagI TCATGA 3 cut(s) 3043, 3511, 3871
PaqCI CACCTGC 1 cut(s) 1880
PceI AGGCCT 2 cut(s) 3219, 3697
PciI ACATGT 2 cut(s) 570, 5149
PciSI GCTCTTC 1 cut(s) 5174
PctI GAATGC 3 cut(s) 3609, 3634, 4871
PdmI GAANNNNTTC 6 cut(s) 96, 623, 641, 3607, 4478, 5712
PflMI CCANNNNNTGG 1 cut(s) 871
PfoI TCCNGGA 2 cut(s) 2121, 5666
PmaCI CACGTG 1 cut(s) 3026
PmeI GTTTAAAC 1 cut(s) 2155
PmlI CACGTG 1 cut(s) 3026
Ppu21I YACGTR 2 cut(s) 3026, 5132
PpuMI RGGWCCY 1 cut(s) 4589
PscI ACATGT 2 cut(s) 570, 5149
PsiI TTATAA 2 cut(s) 4445, 4530
Psp124BI GAGCTC 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 4589
Psp6I CCWGG 5 cut(s) 84, 847, 2121, 3679, 5666
PspCI CACGTG 1 cut(s) 3026
PspFI CCCAGC 1 cut(s) 3320
PspGI CCWGG 5 cut(s) 84, 847, 2121, 3679, 5666
PspN4I GGNNCC 4 cut(s) 47, 977, 4408, 4578
PspPI GGNCC 7 cut(s) 45, 975, 1936, 2119, 2587, 4407, 4589
PspPPI RGGWCCY 1 cut(s) 4589
PspXI VCTCGAGB 2 cut(s) 153, 5166
PstI CTGCAG 3 cut(s) 1737, 2168, 3364
PstNI CAGNNNCTG 2 cut(s) 1256, 4882
PsuI RGATCY 9 cut(s) 1006, 1174, 2125, 2325, 3941, 4489, 4752, 4787, 5294
RseI CAYNNNNRTG 9 cut(s) 821, 1800, 3555, 3870, 4185, 5154, 5258, 5276, 5802
SacI GAGCTC 1 cut(s) 160
SalI GTCGAC 1 cut(s) 4219
SapI GCTCTTC 1 cut(s) 5174
Sau96I GGNCC 7 cut(s) 45, 975, 1936, 2119, 2587, 4407, 4589
ScaI AGTACT 1 cut(s) 663
ScrFI CCNGG 6 cut(s) 86, 849, 1940, 2123, 3681, 5668
SduI GDGCHC 3 cut(s) 160, 472, 5748
SexAI ACCWGGT 1 cut(s) 84
SfcI CTRYAG 8 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360, 5649
Sfr274I CTCGAG 3 cut(s) 153, 2604, 5166
SfuI TTCGAA 1 cut(s) 4852
SinI GGWCC 5 cut(s) 45, 2119, 2587, 4407, 4589
SlaI CTCGAG 3 cut(s) 153, 2604, 5166
SmiMI CAYNNNNRTG 9 cut(s) 821, 1800, 3555, 3870, 4185, 5154, 5258, 5276, 5802
SmlI CTYRAG 8 cut(s) 153, 547, 677, 944, 1698, 2604, 3533, 5166
SmoI CTYRAG 8 cut(s) 153, 547, 677, 944, 1698, 2604, 3533, 5166
SphI GCATGC 2 cut(s) 1361, 5581
SseBI AGGCCT 2 cut(s) 3219, 3697
SsiI CCGC 8 cut(s) 997, 1604, 2570, 3887, 4804, 5184, 5520, 5607
SspI AATATT 4 cut(s) 3455, 3659, 4568, 5415
SstI GAGCTC 1 cut(s) 160
StuI AGGCCT 2 cut(s) 3219, 3697
StyD4I CCNGG 6 cut(s) 84, 847, 1938, 2121, 3679, 5666
StyI CCWWGG 3 cut(s) 1752, 4469, 4651
TaiI ACGT 3 cut(s) 3028, 5134, 5629
TatI WGTACW 9 cut(s) 661, 1229, 1381, 2106, 2686, 2950, 3775, 4341, 5070
TauI GCSGC 3 cut(s) 4807, 5522, 5609
TseFI GTSAC 7 cut(s) 393, 406, 1043, 1435, 1759, 3830, 4496
TseI GCWGC 9 cut(s) 1014, 1303, 1732, 2166, 3119, 3194, 3359, 4516, 5516
Tsp45I GTSAC 7 cut(s) 393, 406, 1043, 1435, 1759, 3830, 4496
TspGWI ACGGA 2 cut(s) 2579, 4934
Van91I CCANNNNNTGG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 547
VpaK11BI GGWCC 5 cut(s) 45, 2119, 2587, 4407, 4589
XagI CCTNNNNNAGG 1 cut(s) 4584
XbaI TCTAGA 3 cut(s) 2671, 4453, 5561
XceI RCATGY 8 cut(s) 129, 574, 820, 1361, 2827, 3470, 5153, 5581
XhoI CTCGAG 3 cut(s) 153, 2604, 5166
XmiI GTMKAC 4 cut(s) 717, 3744, 4220, 5146
XmnI GAANNNNTTC 6 cut(s) 96, 623, 641, 3607, 4478, 5712
ZrmI AGTACT 1 cut(s) 663
Zsp2I ATGCAT 2 cut(s) 4871, 5279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.