Rh3CG307200

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000223
Physical Location & Seq
Forward (+)
32147497 .. 32159528
12032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG307200.1

Sequence Viewer

Length: 8007 bp
ATGGTGGGTTTAACTGAGACTGTTTTTTCTTGGTCCCTTGAAGACATTTTCAATGAAGATCTTTACAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCAGTCCGTTGTCCAGTACTTTGGGTCTTATCTGTATCCTTTATTGGAAGAAACTCGAGCTCAGTTACATTCCAGCATGGAAACTATTGACAGTGCACCATTTGCGGAAGTAGTTGATTTTAAAGAGTCCAAGCCATATGGAACAAAGCAATATACAGTCAAGGTTGATTGTTGGCGAAACAGGTTCAGTGAACGTGGCAATGAACCATACAAAACTTTGCCTGGGGATCTTTTTATTTTAGCAAATGCTAAACCTGAAACTGTTGCTGAATTACAACTGGCTGGGAGGCCATGGTCTTTTGTATCTGTCACTCATCTTGTGTCTAACATCTCACAAATTCAGAAAAAAGATGAGAATGGGGATGAGGTTGAGGATGTGGAAGAGGAAACTTCTCTTTGTTTTAAAATCAAGTCTTCAAAAGAGTTTGAAGTCGATGATGGCACATCAGTGTCTGTTGTTTTCTTGTTGAATTTAATCCCCAGTGAAAGAATATACAAAGCATTGCACATGCTGGGAAATTTAACAATTATCAAAGAAGTTCTATGCTCTGATTCTGTGGCTCAGGAAAATCCTTTCTATGAGAAGAATAATGACATCATGGAGAAGGGGTTAGATGAGAATTTATTGTCAGGACTTAATGAGGCCCAAACTGGGGCAGTTTTGGCTTGTCTTGGAATGCGGCATTGTGGTGCAAAGTCTTCGGTGGAACTTATATGGGGTCCTCCTGGGACAGGAAAAACTAAAACAATCGCTACTCTGCTTTTTACTCTGTTAAAGATGAATTGTAGGACTCTTATTTGTGCCCCAACAAATGTTGCAATAATTGAAGTTGCTTCTCGTCTTCTGAAAATGGTGACAGAAGCAGAGTCCAATGCAATGTTTTGTTCAGTTGGAAAAATCCTTTTATTTGGGAATAAGGAACGACTTAAACTCAAAGTTGGTTCAGATATTGAAGAGATATATTTGGGTCACCGTATTCAAAAAATCATCGAGTGCATAGGACCACAAACTGGTTGGAGGCATTGCTTTGCTTCCATGATAAATTTACTTGAAGATTGTGTTTATGATTACAATATTTTCTTGGGCAATGAGTTGACTGAACTCACTGAAGGGGAAGAACAAAATAGTGCTTGTGAAGTGAATGATGAATGCAGAAGTGGACCAGGAGTTAGTGAGGGGAAGTGCAAAACATTTCTTGAATATTTCAGAACTAGATTTCTCTCTACCGCATCGCCACTAAGATATTGCATTTCTATCTTCTGTACCCATATGACCAAAATCTGCATTCCAGAACACATATTCAAGGATATGGTTTCTCTTATCCTCTTAGTTGATTCCTTTGAGATGATGTTGTTTGAAGGTAATGTTATTTCAGAAGCATTGGAAGGCCTTTTTTCTTGTTCAGAGGTTGAGGGTGTATCTGAGTTATATGTATCTGACTCAATTCCTCTTGTCATGAAGAGAAAGGAATGCCTTGTAGCTTTACGCACTCTACAGTATTCTCTTAGCAAACTTCAACTTCCAGATTTTAGGAAAGGAACTGATCTAATGGATTTATGCTTCCAAGGTGCATCCTTGGTATTTTGCACTGCATCTAGTTCATTTAAGTTGCATAGACTGGAAATGGAGCCATTTACCTTTGTGGTTATTGATGAAGCTGCACAGTTGAAAGAGTGTGAGTCAACTATACCCCTGCAACTACCAGGAGCAAAACATGTAATTCTTGTTGGTGATGAATGCCAATTGCCAGCTATGGTGAAAAGCAATGTTTCTCAAGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCACAACGGGTCACCCAAAACACCTTCTAAATATGCAGTACAGAATGCATCCGTCAATAAGTTTCTTCCCAAATTCTAGTTTCTATAATAACCGGATCCTGGACGCTCCAAATGTCACAAAAAAAAGCCATGAGAAGCACTATCTTCCAGGGCCAATATATGGTCCTTATTCTTTTATAAATGTAAATGATGGAAGAGAGGAGAAGGATGAGGATGGTCATAGTCGAAAGAATATGGTTGAGGTCGCTATTGTTTTGAAAGTACTGCGGAATCTTTACAAAGAATGGATGAATTCAAAACAGAAACTCAGTGTTGGTGTGGTATCTCCCTATGCTGCTCAAGTAGTGGCAATTCAGGAAAATCTTAGAGAAAAGTATGACAATCTTGATGGCTTTAAAGTGAAGGTAAATACAGTTGACGGGTTCCAGGGTGGGGAAGACGACATAATTATAATGTCCACTGTAAGATCCAACAGCCATCAATCACTTCAGTTTATTTCAAATATTCAAAGAGTCAATGTTTCTCTCACAAGGGCTAGGCACTGTCTCTGGATTTTGGGCAATGAAAAAACTCTATGCAGTACTGATTCTGTTTGGAAGGCCTTGGTTCTTGATGCCAAAAGTCGTGACTGCTTCTTTAATGCTGATCAAGACAAGGATTTGGCCAAGGCCATAGTAGACATAAAGAAAGAGTTGGACCAATTGGATGATTTGCTCAATGCCGACAGCATACTTTTCAAAAATGCCAGATGGAAGGTGCTTTTTAGTGATAACTTTTTGAAGTCATTCAAGAAACTGACGTCAGCCTCTTTGAAGAAGTCTGTACTAAACCTTCTACTGAAGCTTTCAACTGGCTGGAGACCTAGGAAGCGCAATGTAGAGACAGGTTGTAGTAGCTCTTCACTAATCTTGAAGAAATTTAAGGTTAAATCTCTATATCTTGTTTGCACAACAGACATTGCAAAGGATATTGTTTGCACAACAGACATTGCAAAGGATATGAGATACATTCAAATATTAAAGGTCTGGGACATATTGCTCCTGGAGGATATCCCAAAGTTGGAATATCGCCTGGAGACTATTCTAAACAGATATACAGATGATTTTATTAGTCTTTGCAAGGAGAAATATTGTGACGGTGATTTAGAAGTTCCACAGAGCTGGCCACTTTCTCTGGAATTTTCCCAGTTCAAGGATCCTAGTGTCACTGAAACCAAGAGAGATTTTGTTGGTGACACTTCTGATGGCAGGAGTTATGTTGAGAATTCACAGGTCAGTGAGAGTTTGTTGCTAATGAAATTTTACTCTTTATCATCTGGTTTGGTGAACCACTTGCTCTCTGACTGTGGGGGTAGAGAATTGGATCTCCCGTTTGAAGTTACAGACCAAGAAAGGGAGATTATACTTCATCATAGAAGTTCTTTTATAGTTGGACGGTCAGGTACTGGGAAGACCACCGTCTTAACCATGAAGTTATTCCAAAGAGAACAATGGCACCATTTGGCTGTGCAGGGATGTTATGGTACTGAAAATAGCAATGATGAGCAAAGTTCTGCAAATACTGAAGGGAAAATTCTACGCCAGCTTTTCGTTACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCAACATGTTACACATTTGAAAAGTTTTGCGTGTGGTGGAAGTGACTCAGCTGAAAGAAGTTTGATTGATATAGTTGATTTTGAAGAGGAGGAATCCCAATTCAAGGATATCCAAGATTCATTTCATGATATTCCTCCGAGTTCTTACCCTCTTGTGATAACATTCTATAAGTTCCTGATGATGCTTGATGGAACATTGAGTAACTCATACTTTGCAAGGTTCTGCGATGCATCACTTTCTCATGGCCAATTTAGAAATTCTAGATCAGTTGTGTTCCAGGCCTTTATAAGGACAAAAGAAGTCAATTATGAGAGGTTTTGTTCATCATATTGGCCCCATTTTAATACTCAGCTAACAAAGAACCTTGATGTTGCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGCTTGGGAACTATAGAAGCATGTGATGGGAAGCTCAGCTTGGAGGATTATGTTAAATTGTCAGAGGGCCGAGCTTCTAATTTAAGCAAGCAAAAGAGAGAACAGATATATGAGATATTTCAAGATTATGAAAAAATGAAGATGCGAAATGGTGAATTTGATCTAGCTGATTTTGTAAATGATCTTCAACGTCGACTCAAGCATGAGAAATATATGGGTGATCAGATGGATTTTATTTATATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTCAAACATATGTGCAACAATGTTGATGAGGGATTTGTTTTTTCTGGTGATACAGCACAAACAATTGCCAGGGGTATTGATTTTAGGTTCCAAGATATACGACATCTGTTCTACAAGAAGTTTGTGTTGGAGTCAAGAAGCAATAAGCAAGATGAAAGAAAAGGAAAAGGCCAAATTTCTGAAATATATCATTTGACTCAAAACTTCCGTACCCATTCTGGTATATTGAAGTTATCGCAGAGCATTATTGAATTACTATATCGCTTTTTTCCTCTTTCAATTGATGTTTTAAAACCAGAAAGCAGTCTGATTTATGGGGAAGCTCCGGTTGTGCTTGAATCTGGAGAAAATGAAAATGCAATCATCAAAATATTTGGAAATAGTAGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATATTGGTGCGAGATGATAGTACTCGAAGAGAAATTTCCAATGTTGTTGGGAAGCATGCTCTTGTCCTTACCATAGTTGAGTGCAAGGGCCTTGAGTTTCAGGATGTACTCTTGTACAACTTTTTCGGCTCATCTCCTTCGAAAAATCAATGGAGGGTTATTTATGATTACATGAAGGAACAAGATTTACTCGACTCTACATTACCTAAGAAATTTCCAAGTTTCAGTGAAGCGAAACACAACATATTATGCTCTGAATTGAAGCAATTATATGTTGCTGTTACTCGCACCAGACAGAGATTGTGGATTTGTGAAACTGTAGAGGAGATTTCGAAACCCATGTTTGACTATTGGAAGAAGAAATGTCTTGTGCATGTTAAGCAGCTTGATGATTCACTTGCACAAGCCATGCAAGTTGCAAGCAGCACAGAGGAGTGGAGATCCCGTGGGATTAAGCTTTATCGTGAGAATAACTACGAGATGGCCACAATGTGCTTTAAAAGATCTGGTGACACTTATTGGGAACAAAGGTCCAAAGCTGCTGGCCTTAAAGCTATTGCTGACCGTATGCGAATGTCAAATCCTGAAGAGGCTAATTCTATTCTTAGGAAAGCTGCTGAAATATTTGATGCTATAGGGAAGGCTGATTCTGCTGCAAGATGTTTTTCTGATTTGGGAGAGTATGAAAGAGCAGCCAGGATTTATTTTGAGAAATGTGGCGAATTGGAAAGAGCCGCTGAATGTTTCACTCTAGCAGGATGCTATGAGGATGCTGCAGATGTGTATGCTAAAGGCAATTTTTTCTTAGAGTGTCTCTCTGTCTGTGCCAAAGGAAAATTATTTGATAGAGGTTTGGAATACATCAATTATTGGAAGAGGCATGCAGTAGAGAACAATTCTTTAACTAGAGGAGGAGAGGGGATACATAATATGGAACAAAAATTTCTAGAGGGCTGTGCACGCCACTATCATAAGAAGAAAGATAACAGATCCATGATGAAATTTGTAAAAGCCTTCCAGTCTATATCATTGATACGGAATTTTTTGAAGAGTTTAGGTAGCCTTGATGAACTTCTTTTGCTGGAAGATCAATTTGGAAACTATTTGGAAGCTGCGGAGATTGCACAGATTAAGGGAGAAATTCTACTTAAATCCGATTATCTTGGAAAGGCAGGTGAATTCAAAGATGCCTCAATGCATATCCTATTCTATGTACTTGCCAGCTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCCATGAAGAAGTTTGCACAACAGGAAGCTCTTTTGAAAAAAACCAAGTCAGTTGCCAAGAATGAGACAGACGACTTTTATGAGTTGGTTTGCACTGAGGTTGATATTTTATTGAATGAACAGGATAATTTGGCTCTGTTGAAACATCAAATGAAGGCTTCTCAGAGACATGCAAGTATTATAGGTGAAGTGTTATCTGCTCGAAAAATGTTGGATTCCCATTTTTCTCTGAGGACTGATGAGTATTTGTGGGAAAAAGAATTGATCAGTGATTGTGTGAAGCATTCTGAAGATATGACATCCCAAAACCAGGTTTCCATCGACTCACTAGTTCACTTCTGGAATATTTGGAAGGAAAAGATTGTTCTCGTGATTGAATGTCTAGGATGCCTTGAAACCCAAGATGTTAATGTAAACAGAAGTTATCAAAAGTTTTGTTTAAACTACTTGGGAGTATGGAGGCTATCTAATCAAATGAATCCTGTGTACGTTTTACTCTTCCCTGATGCTGATTGGGTCAGAGATGTGGACAAAAGTTATTTCCAAAGTCATGGGAAGTTGGTCTCCATTGATGTTCGCCAGCTTATTTCAGCTGCTCAGAAATATTGGAGTTCAGAACTGCTTGCTGTAGGCATTATGGTTTTGGAAAAGCTTGATGAGCTTTTCAAGAATTCTGATTCAACATTCTTCCAGAGCAGGGCTCTTACCTTCATCTATGATGTTGCGGTGTATCTCTTGAATTCCAAATGTTTGAAGAGAAGGCCTCAAGATGCCAAGGCACTGCAGAAATTTGTAACTTTCTCAACTGAAAGGTTTGTTGCTTGCGTGTTTCCTTTAGATTGGAGGGAATCATTTAGAGAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATACATCAGCTCAAAGAACGAGCTGTCTTATGGTCAAATTGGAAGGATCGCAATGACCATTCTCGGGTCTGGTAAGCTGAATTTTGAACTGTGTAAGAAGCTTGTGAAAAAGTTGGACTCCAATCCACCATGGAAGGACTTTATTGAGAACCTATGTGCTAGTACTGAACCTGGAAATACCAGTCAGAAGCCTAGAGAGGTGTCTGTTATGTGGCTATTATCTGAGGCTTTAGTTGAAACTTATGATGCAGACTGGAGGGTTGTTAAAGACTACATATCACCTGGTTGTTTCTTGTATCTTGTGGAGCGTCTTCTGATTTGGGCTTCTCAATGTCGAGGTTATGTTATCACCACAAGTTCTTGTTTCACTGAATGGCTGATGTACCAGGATGAAGATACTCATGTGCAACCATCTATAGAAGATACTCATGTGCAACCATCTATAGAAGGCATCCTTCATTTTGTTAGTAATGTGGTTACCGAATGCCTTTGGGACAAGGCAAATCTGATTGAATGGATTAGAAAGTCTAATGCAGATTGGAAGACCTGTTATTCACTACTCGTGCCAAGATTGGTGTTTGTATTATGTTTGCTTTGTGTGAACTTTGGAATATCCTTGGGTGTACTGTCTGATATGCTGGGAAGCAGCTATATCACTGAACACCTTCCATCGGAATTTTACGACACCCTTAAGAGGCGGAGGAGGAACAATTTTCTGAATATAAATGAGGAAGTGCTTGCAGCAGCTCTCAAGAAGATTCATAATCCTTTGGTAATTGCGAGTTTTGGGTTTGATTGTTCAAGGTTCGTATGCCCAGATGCCATCTTTGTTGACATGAAGGCCATTCAGTCCAAGGATGACCTATTCAGAATAATGTTTCCTAAACCACCAGTCATTCAAGTTGCACCAGGTCGAATTGGATTTGTTGAAGCTGGAGGTAATACTCTTTGTGATGGTGTACTTTCTGCAAATGATATCGACGCAGGGAAGAGCTCTTTAAACTCCAACTTGGTGGGAGAAACTGATGAGCAGCTTGATGCTGAAGACGGTCAGAGTAGAGGTGAGGAAATCTGCAAAGCCGGTGTGTCTACACCAATGTGTCCACATCCTGATTCTACTTCTGCAACCCAAGCAAACAAAAAGGGCAACCGCAAGAATAATACTAAGAAAAATAAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2668

Amino Acids

303.87

Weight (kDa)

5.91

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 66 - 207 3.3e-46 Domain of unknown function (DUF6469)
AAA_11 PF13086 245 - 622 2.3e-32 AAA domain
AAA_19 PF13245 265 - 618 3e-09 AAA domain
AAA_12 PF13087 630 - 826 5.7e-57 AAA domain
UvrD-helicase PF00580 1343 - 1432 2.1e-08 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2090, 2363, 3824
AarI CACCTGC 2 cut(s) 4633, 5810
AatII GACGTC 1 cut(s) 2714
Acc36I ACCTGC 2 cut(s) 4633, 5810
AccB1I GGYRCC 1 cut(s) 3405
AccB7I CCANNNNNTGG 2 cut(s) 1109, 2072
AccI GTMKAC 3 cut(s) 2589, 4141, 7913
AciI CCGC 9 cut(s) 203, 778, 1326, 2179, 5382, 5762, 6626, 7522, 7975
AcoI YGGCCR 4 cut(s) 2574, 3074, 3781, 5130
AcuI CTGAAG 7 cut(s) 1227, 2384, 2771, 3495, 5253, 6210, 7887
AcyI GRCGYC 1 cut(s) 2711
AdeI CACNNNGTG 1 cut(s) 5139
AflII CTTAAG 1 cut(s) 7514
AflIII ACRYGT 2 cut(s) 1813, 3541
AhlI ACTAGT 1 cut(s) 6229
AjuI GAANNNNNNNTTGG 8 cut(s) 3642, 3674, 3971, 4003, 4340, 4372, 5724, 5756
AleI CACNNNNGTG 2 cut(s) 545, 7921
Alw21I GWGCWC 4 cut(s) 160, 196, 5608, 7820
Alw26I GTCTC 9 cut(s) 11, 2462, 2764, 2786, 2981, 5465, 5960, 6061, 6469
Alw44I GTGCAC 2 cut(s) 192, 5604
AlwNI CAGNNNCTG 2 cut(s) 551, 3356
Ama87I CYCGRG 2 cut(s) 153, 6887
ApaLI GTGCAC 2 cut(s) 192, 5604
Asp700I GAANNNNTTC 5 cut(s) 84, 96, 2696, 3386, 7488
AspA2I CCTAGG 1 cut(s) 2774
AspLEI GCGC 1 cut(s) 2784
AsuII TTCGAA 2 cut(s) 4787, 4979
AvaI CYCGRG 2 cut(s) 153, 6887
AvaII GGWCC 7 cut(s) 33, 818, 1100, 1259, 2075, 2608, 5178
AvrII CCTAGG 1 cut(s) 2774
BaeGI GKGCMC 3 cut(s) 196, 904, 5608
BaeI ACNNNNGTAYC 6 cut(s) 4423, 4456, 7212, 7239, 7245, 7272
BalI TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
BamHI GGATCC 2 cut(s) 2007, 3106
BanI GGYRCC 1 cut(s) 3405
BanII GRGCYC 3 cut(s) 160, 6604, 7820
BarI GAAGNNNNNNTAC 6 cut(s) 2727, 2759, 2794, 2826, 4116, 4148
BauI CACGAG 2 cut(s) 6269, 7385
BbsI GAAGAC 9 cut(s) 48, 504, 789, 932, 2355, 3368, 7127, 7373, 7875
Bbv12I GWGCWC 4 cut(s) 160, 196, 5608, 7820
BcgI CGANNNNNNTGC 2 cut(s) 780, 814
BciVI GTATCC 2 cut(s) 144, 5562
BclI TGATCA 3 cut(s) 2557, 4168, 6165
BcoDI GTCTC 9 cut(s) 11, 2462, 2764, 2786, 2981, 5465, 5960, 6061, 6469
BcuI ACTAGT 1 cut(s) 6229
BfmI CTRYAG 9 cut(s) 1592, 3960, 4965, 5280, 5421, 6528, 6683, 7239, 7266
BfrI CTTAAG 1 cut(s) 7514
BfuAI ACCTGC 2 cut(s) 4633, 5810
BfuI GTATCC 2 cut(s) 144, 5562
BglII AGATCT 3 cut(s) 58, 4204, 5150
BlnI CCTAGG 1 cut(s) 2774
BlpI GCTNAGC 1 cut(s) 3983
BmcAI AGTACT 5 cut(s) 116, 2175, 2494, 4669, 6988
Bme18I GGWCC 7 cut(s) 33, 818, 1100, 1259, 2075, 2608, 5178
BmeT110I CYCGRG 2 cut(s) 153, 6887
BmiI GGNNCC 9 cut(s) 35, 819, 1728, 2009, 2336, 3108, 3407, 3872, 4319
BmrI ACTGGG 4 cut(s) 573, 759, 3091, 3366
BmuI ACTGGG 4 cut(s) 573, 759, 3091, 3366
BoxI GACNNNNGTC 1 cut(s) 3368
BpiI GAAGAC 9 cut(s) 48, 504, 789, 932, 2355, 3368, 7127, 7373, 7875
BplI GAGNNNNNCTC 6 cut(s) 5447, 5479, 6586, 6618, 6649, 6681
BpmI CTGGAG 6 cut(s) 2788, 2975, 3005, 4593, 7099, 7779
Bpu10I CCTNAGC 2 cut(s) 660, 3516
Bpu1102I GCTNAGC 1 cut(s) 3983
Bpu14I TTCGAA 2 cut(s) 4787, 4979
BpuEI CTTGAG 6 cut(s) 1857, 2235, 4130, 4760, 6651, 7559
BsaHI GRCGYC 1 cut(s) 2711
BsaI GGTCTC 2 cut(s) 2764, 6469
BsaWI WCCGGW 2 cut(s) 2004, 4555
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 5077, 5107
Bse118I RCCGGY 1 cut(s) 7904
BseRI GAGGAG 7 cut(s) 2126, 3638, 4985, 5093, 5571, 5574, 7540
BseSI GKGCMC 3 cut(s) 196, 904, 5608
BseYI CCCAGC 3 cut(s) 380, 610, 7462
BsgI GTGCAG 3 cut(s) 1743, 3440, 4656
BshNI GGYRCC 1 cut(s) 3405
BsiHKAI GWGCWC 4 cut(s) 160, 196, 5608, 7820
BsiHKCI CYCGRG 2 cut(s) 153, 6887
BsiSI CCGG 3 cut(s) 2005, 4556, 7905
BslFI GGGAC 4 cut(s) 19, 841, 2954, 7331
BsmAI GTCTC 9 cut(s) 11, 2462, 2764, 2786, 2981, 5465, 5960, 6061, 6469
BsmFI GGGAC 4 cut(s) 19, 841, 2954, 7331
BsmI GAATGC 8 cut(s) 780, 1253, 1383, 1574, 1841, 1962, 6184, 7313
Bso31I GGTCTC 2 cut(s) 2764, 6469
BsoBI CYCGRG 2 cut(s) 153, 6887
Bsp119I TTCGAA 2 cut(s) 4787, 4979
Bsp1286I GDGCHC 6 cut(s) 160, 196, 904, 5608, 6604, 7820
Bsp1407I TGTACA 1 cut(s) 4761
Bsp1720I GCTNAGC 1 cut(s) 3983
Bsp19I CCATGG 2 cut(s) 389, 6953
BspACI CCGC 9 cut(s) 203, 778, 1326, 2179, 5382, 5762, 6626, 7522, 7975
BspHI TCATGA 2 cut(s) 1554, 3661
BspLI GGNNCC 9 cut(s) 35, 819, 1728, 2009, 2336, 3108, 3407, 3872, 4319
BspMAI CTGCAG 2 cut(s) 5425, 6687
BspMI ACCTGC 2 cut(s) 4633, 5810
BspQI GCTCTTC 2 cut(s) 2815, 7808
BspT104I TTCGAA 2 cut(s) 4787, 4979
BspT107I GGYRCC 1 cut(s) 3405
BspTI CTTAAG 1 cut(s) 7514
BspTNI GGTCTC 2 cut(s) 2764, 6469
BsrFI RCCGGY 1 cut(s) 7904
BsrGI TGTACA 1 cut(s) 4761
BssAI RCCGGY 1 cut(s) 7904
BssNI GRCGYC 1 cut(s) 2711
BssSI CACGAG 2 cut(s) 6269, 7385
Bst2BI CACGAG 2 cut(s) 6269, 7385
BstACI GRCGYC 1 cut(s) 2711
BstAFI CTTAAG 1 cut(s) 7514
BstAPI GCANNNNNTGC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 4761
BstBI TTCGAA 2 cut(s) 4787, 4979
BstDSI CCRYGG 3 cut(s) 389, 5092, 6953
BstEII GGTNACC 3 cut(s) 1067, 1922, 7300
BstENI CCTNNNNNAGG 2 cut(s) 828, 3823
BstHHI GCGC 1 cut(s) 2784
BstMAI GTCTC 9 cut(s) 11, 2462, 2764, 2786, 2981, 5465, 5960, 6061, 6469
BstNSI RCATGY 8 cut(s) 610, 1817, 3545, 3972, 4706, 5024, 5531, 6074
BstPAI GACNNNNGTC 1 cut(s) 3368
BstPI GGTNACC 3 cut(s) 1067, 1922, 7300
BstSFI CTRYAG 9 cut(s) 1592, 3960, 4965, 5280, 5421, 6528, 6683, 7239, 7266
BstSLI GKGCMC 3 cut(s) 196, 904, 5608
BstV2I GAAGAC 9 cut(s) 48, 504, 789, 932, 2355, 3368, 7127, 7373, 7875
BstXI CCANNNNNNTGG 4 cut(s) 119, 3072, 6452, 7837
BsuI GTATCC 2 cut(s) 144, 5562
BtgI CCRYGG 3 cut(s) 389, 5092, 6953
BtgZI GCGATG 2 cut(s) 1314, 3777
BtsI GCAGTG 2 cut(s) 1686, 6680
BveI ACCTGC 2 cut(s) 4633, 5810
CaiI CAGNNNCTG 2 cut(s) 551, 3356
CciI TCATGA 2 cut(s) 1554, 3661
CfoI GCGC 1 cut(s) 2784
Cfr10I RCCGGY 1 cut(s) 7904
CseI GACGC 3 cut(s) 2024, 7121, 7814
CsiI ACCWGGT 3 cut(s) 6210, 7105, 7732
DraI TTTAAA 7 cut(s) 220, 502, 2308, 4521, 5146, 6342, 7824
DraIII CACNNNGTG 1 cut(s) 5139
EaeI YGGCCR 4 cut(s) 2574, 3074, 3781, 5130
EciI GGCGGA 1 cut(s) 7537
Ecl136II GAGCTC 2 cut(s) 158, 7818
Eco147I AGGCCT 4 cut(s) 1488, 2513, 3818, 6664
Eco24I GRGCYC 3 cut(s) 160, 6604, 7820
Eco31I GGTCTC 2 cut(s) 2764, 6469
Eco32I GATATC 3 cut(s) 2962, 3646, 7801
Eco47I GGWCC 7 cut(s) 33, 818, 1100, 1259, 2075, 2608, 5178
Eco53kI GAGCTC 2 cut(s) 158, 7818
Eco57I CTGAAG 7 cut(s) 1227, 2384, 2771, 3495, 5253, 6210, 7887
Eco88I CYCGRG 2 cut(s) 153, 6887
Eco91I GGTNACC 3 cut(s) 1067, 1922, 7300
EcoICRI GAGCTC 2 cut(s) 158, 7818
EcoNI CCTNNNNNAGG 2 cut(s) 828, 3823
EcoO109I RGGNCCY 2 cut(s) 818, 4735
EcoO65I GGTNACC 3 cut(s) 1067, 1922, 7300
EcoRI GAATTC 5 cut(s) 2203, 3175, 5825, 6571, 6640
EcoRV GATATC 3 cut(s) 2962, 3646, 7801
EcoT22I ATGCAT 3 cut(s) 1962, 3769, 5847
EcoT38I GRGCYC 3 cut(s) 160, 6604, 7820
FalI AAGNNNNNCTT 4 cut(s) 3971, 4003, 7263, 7295
FaqI GGGAC 4 cut(s) 19, 841, 2954, 7331
FauNDI CATATG 3 cut(s) 235, 1368, 4239
FbaI TGATCA 3 cut(s) 2557, 4168, 6165
FblI GTMKAC 3 cut(s) 2589, 4141, 7913
FriOI GRGCYC 3 cut(s) 160, 6604, 7820
GlaI GCGC 1 cut(s) 2783
GsaI CCCAGC 3 cut(s) 384, 614, 7466
GsuI CTGGAG 6 cut(s) 2788, 2975, 3005, 4593, 7099, 7779
HapII CCGG 3 cut(s) 2005, 4556, 7905
HgaI GACGC 3 cut(s) 2024, 7121, 7814
HhaI GCGC 1 cut(s) 2784
Hin1I GRCGYC 1 cut(s) 2711
Hin6I GCGC 1 cut(s) 2782
HinP1I GCGC 1 cut(s) 2782
HincII GTYRAC 5 cut(s) 1194, 1782, 2329, 4142, 7657
HindII GTYRAC 5 cut(s) 1194, 1782, 2329, 4142, 7657
HindIII AAGCTT 5 cut(s) 1894, 2753, 5102, 6551, 6923
HpaII CCGG 3 cut(s) 2005, 4556, 7905
Hpy99I CGWCG 2 cut(s) 4143, 7808
HpyCH4IV ACGT 4 cut(s) 292, 2711, 4138, 6390
HpySE526I ACGT 4 cut(s) 292, 2711, 4138, 6390
Hsp92I GRCGYC 1 cut(s) 2711
HspAI GCGC 1 cut(s) 2782
Ksp22I TGATCA 3 cut(s) 2557, 4168, 6165
LguI GCTCTTC 2 cut(s) 2815, 7808
LmnI GCTCC 6 cut(s) 1726, 1805, 2023, 2955, 4558, 7129
MabI ACCWGGT 3 cut(s) 6210, 7105, 7732
MaeII ACGT 4 cut(s) 292, 2711, 4138, 6390
MfeI CAATTG 4 cut(s) 1841, 2612, 4293, 4509
MhlI GDGCHC 6 cut(s) 160, 196, 904, 5608, 6604, 7820
MlsI TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
MluNI TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
Mox20I TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
Mph1103I ATGCAT 3 cut(s) 1962, 3769, 5847
MroXI GAANNNNTTC 5 cut(s) 84, 96, 2696, 3386, 7488
MscI TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
MslI CAYNNNNRTG 3 cut(s) 545, 786, 7921
Msp20I TGGCCA 4 cut(s) 2576, 3076, 3783, 5132
MspA1I CMGCKG 3 cut(s) 3587, 5384, 6494
MspCI CTTAAG 1 cut(s) 7514
MspI CCGG 3 cut(s) 2005, 4556, 7905
MssI GTTTAAAC 1 cut(s) 6342
MunI CAATTG 4 cut(s) 1841, 2612, 4293, 4509
Mva1269I GAATGC 8 cut(s) 780, 1253, 1383, 1574, 1841, 1962, 6184, 7313
NcoI CCATGG 2 cut(s) 389, 6953
NdeI CATATG 3 cut(s) 235, 1368, 4239
NlaIV GGNNCC 9 cut(s) 35, 819, 1728, 2009, 2336, 3108, 3407, 3872, 4319
NmeAIII GCCGAG 1 cut(s) 4043
NsiI ATGCAT 3 cut(s) 1962, 3769, 5847
NspI RCATGY 8 cut(s) 610, 1817, 3545, 3972, 4706, 5024, 5531, 6074
NspV TTCGAA 2 cut(s) 4787, 4979
OliI CACNNNNGTG 2 cut(s) 545, 7921
PaeI GCATGC 2 cut(s) 4706, 5531
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 2 cut(s) 1554, 3661
PaqCI CACCTGC 2 cut(s) 4633, 5810
PceI AGGCCT 4 cut(s) 1488, 2513, 3818, 6664
PciI ACATGT 2 cut(s) 1813, 3541
PciSI GCTCTTC 2 cut(s) 2815, 7808
PctI GAATGC 8 cut(s) 780, 1253, 1383, 1574, 1841, 1962, 6184, 7313
PdmI GAANNNNTTC 5 cut(s) 84, 96, 2696, 3386, 7488
PflMI CCANNNNNTGG 2 cut(s) 1109, 2072
PfoI TCCNGGA 2 cut(s) 2010, 2952
PmeI GTTTAAAC 1 cut(s) 6342
PpuMI RGGWCCY 1 cut(s) 818
PscI ACATGT 2 cut(s) 1813, 3541
PshAI GACNNNNGTC 1 cut(s) 3368
PsiI TTATAA 3 cut(s) 2090, 2363, 3824
Psp124BI GAGCTC 2 cut(s) 160, 7820
Psp5II RGGWCCY 1 cut(s) 818
PspEI GGTNACC 3 cut(s) 1067, 1922, 7300
PspFI CCCAGC 3 cut(s) 380, 610, 7462
PspN4I GGNNCC 9 cut(s) 35, 819, 1728, 2009, 2336, 3108, 3407, 3872, 4319
PspPPI RGGWCCY 1 cut(s) 818
PspXI VCTCGAGB 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 4818, 4850
PstI CTGCAG 2 cut(s) 5425, 6687
PstNI CAGNNNCTG 2 cut(s) 551, 3356
PvuII CAGCTG 2 cut(s) 3587, 6494
RseI CAYNNNNRTG 3 cut(s) 545, 786, 7921
SacI GAGCTC 2 cut(s) 160, 7820
SalI GTCGAC 1 cut(s) 4140
SapI GCTCTTC 2 cut(s) 2815, 7808
ScaI AGTACT 5 cut(s) 116, 2175, 2494, 4669, 6988
SduI GDGCHC 6 cut(s) 160, 196, 904, 5608, 6604, 7820
SexAI ACCWGGT 3 cut(s) 6210, 7105, 7732
SfcI CTRYAG 9 cut(s) 1592, 3960, 4965, 5280, 5421, 6528, 6683, 7239, 7266
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 4787, 4979
SinI GGWCC 7 cut(s) 33, 818, 1100, 1259, 2075, 2608, 5178
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 3 cut(s) 545, 786, 7921
SmlI CTYRAG 8 cut(s) 153, 1872, 2250, 4145, 4739, 6666, 7514, 7574
SmoI CTYRAG 8 cut(s) 153, 1872, 2250, 4145, 4739, 6666, 7514, 7574
SpeI ACTAGT 1 cut(s) 6229
SphI GCATGC 2 cut(s) 4706, 5531
SseBI AGGCCT 4 cut(s) 1488, 2513, 3818, 6664
SsiI CCGC 9 cut(s) 203, 778, 1326, 2179, 5382, 5762, 6626, 7522, 7975
SspI AATATT 9 cut(s) 1174, 1301, 2416, 2928, 3041, 4602, 5271, 6247, 6506
SstI GAGCTC 2 cut(s) 160, 7820
StuI AGGCCT 4 cut(s) 1488, 2513, 3818, 6664
TaiI ACGT 4 cut(s) 295, 2714, 4141, 6393
TauI GCSGC 2 cut(s) 781, 5384
TspGWI ACGGA 4 cut(s) 94, 1953, 4427, 5698
Van91I CCANNNNNTGG 2 cut(s) 1109, 2072
Vha464I CTTAAG 1 cut(s) 7514
VneI GTGCAC 2 cut(s) 192, 5604
VpaK11BI GGWCC 7 cut(s) 33, 818, 1100, 1259, 2075, 2608, 5178
XagI CCTNNNNNAGG 2 cut(s) 828, 3823
XbaI TCTAGA 2 cut(s) 3797, 5593
XceI RCATGY 8 cut(s) 610, 1817, 3545, 3972, 4706, 5024, 5531, 6074
XcmI CCANNNNNNNNNTGG 1 cut(s) 1110
XhoI CTCGAG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 2774
XmiI GTMKAC 3 cut(s) 2589, 4141, 7913
XmnI GAANNNNTTC 5 cut(s) 84, 96, 2696, 3386, 7488
ZraI GACGTC 1 cut(s) 2712
ZrmI AGTACT 5 cut(s) 116, 2175, 2494, 4669, 6988
Zsp2I ATGCAT 3 cut(s) 1962, 3769, 5847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.