Rorug04G0366600

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000223
Physical Location & Seq
Reverse (-)
53793460 .. 53803358
9899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0366600.1

Sequence Viewer

Length: 2523 bp
ATGACGGAGAAGCAGTTGATCGTGGTCGTTGAAGGCACGGCGGCCATCGGGCCTTACTGGTCAGCCGTCGTCTCCGATTACGTCGAGAAGATCATCAGGTCTTTCTCTGGAAACGAGTTAAGTGGTCAGAAGCCCTCCACTTCTATTGTTGAGATGTCTTTGGTCACATTCAATACTCATGGATCTTATTCCGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGATGTTGATCGTTTCTTACAATGGCTGTCAGCTATTCCCTTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCCTAATGATGTTCCCTACTGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCTGCTAGTAACCCTCATCCCCTGCCCACTCCAGTCTATAGACCACAAATTCAAAATGTGGAGAAGAGTGAAAATATTGATGCACAAACCGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCCTTGTTTCTCTGTCAGTCATCTGTCCGAAACAGCTTCCTAAACTCAGAGCCATTTACAATGCGGGAAAGCGCAACCCCCGGGCGGCAGATCCACCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATGGAGGCCCGGGCTACTTTAAGTCGTCCCGGAAGTACAACTTTGCCCTCAAATCCGAGTACTGTAAAGATGGACATAGCTCCTGTTGCATCAGTGACAGGGCCACCTCCAACTTCTATGCCATCCGTGAATGGATCTGTTATGAATCGGCCATCAGTTTCTGTTAACAATGTGCCTCCTGCGACAGTGAAAGTGGAGCCAACCACTGTAAGTTCAATGGTAGCTGGACCTGCTTTTGCACATGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGATTACAAACAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTAATGTGACACATCAACTACGTCCTGTGGGTAGCATCCTCAATAATATCTCTCAAGCACGCGTGATGAATTCTGCGGCCTTATCTGGAGGATCTTCTATAGGTCTCCAAAACATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCGGTGGCTGCTGCTCAAAATGTATTTTCATCTTCAGGGCCTCACACTCTTGCACAGGTTGCTCAGAGCACAGGTCTTGGTTCATTTACTTCAGGTACTTCCAATGTGTCTGGAAATTCTAACCTTGGGATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGCATCGGCCAATCTGTTCCTGGCATGAGTCAAGGAAATCTCTCAGGACCCCAGATGGTTCAAAGTGGCATTGGAATGACTCCAAACATGATGAGTGGCCTTGGTTCATCAGGTACCTCCTCTGGAACTGGGACAATGATTCCCACTCCAGGCATGTCTCAACAGGCACAATCTGGGATGCAGTCACTTGGTGCAAACAATAGTTCAGTTTCTAATGTGCCGTTGTCACAACAGACGTCAAGTGCTCTGCAGTCTGCACAATCCAAATATGTGAAAGTGTGGGAGGGAAACTTATCTGGGCAGAGACAAGGGCAACCTGTGTTTATTACTAGATTGGAGGGATACAGAAGTGCTACAGCATCTGAGACGCTTGCAGCAAACTGGCCACCGACAATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTCATCCAATTGCCGTCTCAGACACTACTGCTCTCTGTTTCGGACAAAGCTTGCCGCTTGATTGGAATGCTTTTCCCAGGGCTAAGTCTACCATCGGTTCAGGATATGGTTGTGTTTAAGCCACAAGTAGGCGGTCAGCAGCAGCAGATGCAACAAATGCAACAACAGCAGCCGCAGCTACAACAGATGCAGCAACAGCAGCCGCAGCTCCAGCAACAACAGCAACCGCAACTGCAGCAGCATCCGCAAATGCAACAACAGCAACAGCAACCGCAACTGCAGCAGCAGCAGCATCCGCAGATGCAACAACAGCAGCAGCATCCACAGATGCAACAACAGCAGCATCAGCAGCATCCACAGATGCAACAGCAGCAGCAGCAGCAGCTTCCGCAGATGCAACAACAGCAGCAACATCCTCAGATGCAACAGCAGCAGCTCTCGCAGCTCCAGTCACAACAGCAAATTCCACAACTGCAGCAACTGCAGCAACAACACCAACAGCAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTACTTTCGCAAGGACAAGTTTCATCACAACAAGGGCCTAATATGCCAGGAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

840

Amino Acids

90.5

Weight (kDa)

9.13

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 7e-44 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1610
Acc36I ACCTGC 2 cut(s) 260, 922
Acc65I GGTACC 1 cut(s) 1484
AccB1I GGYRCC 1 cut(s) 1484
AccB7I CCANNNNNTGG 2 cut(s) 980, 1147
AccI GTMKAC 1 cut(s) 1987
AccII CGCG 1 cut(s) 1095
AclWI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
AcoI YGGCCR 5 cut(s) 42, 833, 1164, 1378, 1754
AcsI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2361
AcuI CTGAAG 3 cut(s) 333, 1224, 1281
AcyI GRCGYC 1 cut(s) 1607
AdeI CACNNNGTG 1 cut(s) 1562
AfaI GTAC 5 cut(s) 204, 721, 745, 1303, 1486
AfiI CCNNNNNNNGG 8 cut(s) 269, 619, 944, 980, 1147, 1520, 2027, 2507
AflIII ACRYGT 2 cut(s) 925, 1093
AgsI TTSAA 8 cut(s) 32, 172, 341, 377, 449, 900, 1021, 1433
AjnI CCWGG 4 cut(s) 1390, 1519, 1975, 2500
Alw21I GWGCWC 2 cut(s) 1277, 1618
Alw26I GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
AlwI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
AlwNI CAGNNNCTG 3 cut(s) 845, 1733, 2380
Ama87I CYCGRG 2 cut(s) 615, 693
ApoI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2361
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
Asp718I GGTACC 1 cut(s) 1484
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2489
AsuC2I CCSGG 5 cut(s) 616, 617, 694, 695, 714
AsuHPI GGTGA 2 cut(s) 963, 995
AvaI CYCGRG 2 cut(s) 615, 693
AvaII GGWCC 2 cut(s) 911, 1418
BaeI ACNNNNGTAYC 2 cut(s) 1705, 1738
BalI TGGCCA 2 cut(s) 1166, 1756
BanI GGYRCC 1 cut(s) 1484
Bbv12I GWGCWC 2 cut(s) 1277, 1618
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 7 cut(s) 53, 748, 814, 844, 982, 1420, 1999
BceAI ACGGC 5 cut(s) 50, 54, 477, 1576, 1897
BciT130I CCWGG 4 cut(s) 1392, 1521, 1977, 2502
BciVI GTATCC 1 cut(s) 1706
BcnI CCSGG 5 cut(s) 616, 617, 694, 695, 714
BcoDI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BfaI CTAG 3 cut(s) 402, 1701, 1835
BfmI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2132, 2177, 2372, 2381
BfuAI ACCTGC 2 cut(s) 260, 922
BfuI GTATCC 1 cut(s) 1706
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1392, 1521, 1977, 2502
Bme18I GGWCC 2 cut(s) 911, 1418
BmeT110I CYCGRG 2 cut(s) 615, 693
BmgT120I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2489
BmiI GGNNCC 3 cut(s) 882, 1420, 1486
BmrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1392, 1521, 1977, 2502
BmrI ACTGGG 1 cut(s) 1509
BmuI ACTGGG 1 cut(s) 1509
BpmI CTGGAG 5 cut(s) 411, 1140, 1503, 2093, 2330
Bpu10I CCTNAGC 2 cut(s) 265, 507
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 5 cut(s) 616, 617, 694, 695, 714
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 1607
BsaI GGTCTC 1 cut(s) 1142
BsaJI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 8 cut(s) 269, 619, 944, 980, 1147, 1520, 2027, 2507
Bse118I RCCGGY 1 cut(s) 2445
Bse1I ACTGG 5 cut(s) 62, 428, 1504, 1757, 2347
Bse3DI GCAATG 2 cut(s) 382, 1854
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 4 cut(s) 1392, 1521, 1977, 2502
BseDI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 8 cut(s) 269, 619, 944, 980, 1147, 1520, 2027, 2507
BseMI GCAATG 2 cut(s) 382, 1854
BseMII CTCAG 9 cut(s) 498, 595, 687, 1283, 1428, 1725, 1802, 1931, 2330
BseNI ACTGG 5 cut(s) 62, 428, 1504, 1757, 2347
BseRI GAGGAG 2 cut(s) 1480, 2520
BsgI GTGCAG 1 cut(s) 1611
Bsh1236I CGCG 1 cut(s) 1095
BshNI GGYRCC 1 cut(s) 1484
BsiHKAI GWGCWC 2 cut(s) 1277, 1618
BsiHKCI CYCGRG 2 cut(s) 615, 693
BsiSI CCGG 5 cut(s) 192, 616, 694, 714, 2446
BslFI GGGAC 2 cut(s) 696, 1516
BslI CCNNNNNNNGG 8 cut(s) 269, 619, 944, 980, 1147, 1520, 2027, 2507
BsmAI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BsmBI CGTCTC 3 cut(s) 76, 1730, 1920
BsmFI GGGAC 2 cut(s) 696, 1516
BsmI GAATGC 2 cut(s) 387, 1971
Bso31I GGTCTC 1 cut(s) 1142
BsoBI CYCGRG 2 cut(s) 615, 693
Bsp1286I GDGCHC 2 cut(s) 1277, 1618
Bsp143I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BspCNI CTCAG 9 cut(s) 499, 594, 686, 1282, 1427, 1726, 1801, 1930, 2329
BspFNI CGCG 1 cut(s) 1095
BspLI GGNNCC 3 cut(s) 882, 1420, 1486
BspMAI CTGCAG 5 cut(s) 1623, 2136, 2181, 2376, 2385
BspMI ACCTGC 2 cut(s) 260, 922
BspPI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
BspT107I GGYRCC 1 cut(s) 1484
BspTNI GGTCTC 1 cut(s) 1142
BsrDI GCAATG 2 cut(s) 382, 1854
BsrFI RCCGGY 1 cut(s) 2445
BsrI ACTGG 5 cut(s) 62, 428, 1504, 1757, 2347
BssAI RCCGGY 1 cut(s) 2445
BssECI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BssMI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BssNI GRCGYC 1 cut(s) 1607
BssT1I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Bst2UI CCWGG 4 cut(s) 1392, 1521, 1977, 2502
Bst4CI ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2406
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 1607
BstAPI GCANNNNNTGC 4 cut(s) 1265, 2047, 2056, 2380
BstC8I GCNNGC 3 cut(s) 1093, 1743, 1951
BstENI CCTNNNNNAGG 1 cut(s) 942
BstFNI CGCG 1 cut(s) 1095
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 8 cut(s) 21, 93, 185, 235, 628, 821, 1127, 1795
BstMAI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BstMBI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BstNI CCWGG 4 cut(s) 1392, 1521, 1977, 2502
BstNSI RCATGY 3 cut(s) 929, 1175, 1528
BstSCI CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1390, 1519, 1975, 2500
BstSFI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2132, 2177, 2372, 2381
BstUI CGCG 1 cut(s) 1095
BstX2I RGATCY 4 cut(s) 182, 625, 818, 1124
BstYI RGATCY 4 cut(s) 182, 625, 818, 1124
BsuI GTATCC 1 cut(s) 1706
BtsIMutI CAGTG 4 cut(s) 539, 783, 876, 888
BveI ACCTGC 2 cut(s) 260, 922
Cac8I GCNNGC 3 cut(s) 1093, 1743, 1951
CaiI CAGNNNCTG 3 cut(s) 845, 1733, 2380
CfoI GCGC 1 cut(s) 609
Cfr10I RCCGGY 1 cut(s) 2445
Cfr13I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2489
Cfr9I CCCGGG 2 cut(s) 615, 693
CseI GACGC 1 cut(s) 1747
Csp6I GTAC 5 cut(s) 203, 720, 744, 1302, 1485
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviQI GTAC 5 cut(s) 203, 720, 744, 1302, 1485
DpnI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1126, 1794
DpnII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
DraIII CACNNNGTG 1 cut(s) 1562
EaeI YGGCCR 5 cut(s) 42, 833, 1164, 1378, 1754
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco130I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Eco31I GGTCTC 1 cut(s) 1142
Eco32I GATATC 1 cut(s) 1188
Eco47I GGWCC 2 cut(s) 911, 1418
Eco57I CTGAAG 3 cut(s) 333, 1224, 1281
Eco88I CYCGRG 2 cut(s) 615, 693
EcoNI CCTNNNNNAGG 1 cut(s) 942
EcoO109I RGGNCCY 3 cut(s) 1244, 1418, 2489
EcoRI GAATTC 1 cut(s) 1102
EcoRII CCWGG 4 cut(s) 1390, 1519, 1975, 2500
EcoRV GATATC 1 cut(s) 1188
EcoT14I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
EcoT22I ATGCAT 1 cut(s) 1173
ErhI CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Esp3I CGTCTC 3 cut(s) 76, 1730, 1920
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FaqI GGGAC 2 cut(s) 696, 1516
FauI CCCGC 1 cut(s) 592
FblI GTMKAC 1 cut(s) 1987
FspBI CTAG 3 cut(s) 402, 1701, 1835
GlaI GCGC 1 cut(s) 608
GsuI CTGGAG 5 cut(s) 411, 1140, 1503, 2093, 2330
HapII CCGG 5 cut(s) 192, 616, 694, 714, 2446
HgaI GACGC 1 cut(s) 1747
HhaI GCGC 1 cut(s) 609
Hin1I GRCGYC 1 cut(s) 1607
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 3 cut(s) 1889, 1947, 2430
HinfI GANTC 5 cut(s) 829, 1399, 1450, 1510, 1852
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 5 cut(s) 192, 616, 694, 714, 2446
HphI GGTGA 2 cut(s) 963, 995
Hpy166II GTNNAC 2 cut(s) 850, 1988
Hpy188III TCNNGA 8 cut(s) 85, 108, 1119, 1317, 1416, 1494, 1790, 2000
Hpy8I GTNNAC 2 cut(s) 850, 1988
Hpy99I CGWCG 2 cut(s) 71, 86
HpyAV CCTTC 4 cut(s) 26, 296, 948, 1815
HpyCH4III ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2406
HpyCH4IV ACGT 3 cut(s) 81, 1054, 1607
HpySE526I ACGT 3 cut(s) 81, 1054, 1607
Hsp92I GRCGYC 1 cut(s) 1607
HspAI GCGC 1 cut(s) 607
KpnI GGTACC 1 cut(s) 1488
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
LmnI GCTCC 4 cut(s) 769, 880, 2112, 2349
MaeI CTAG 3 cut(s) 402, 1701, 1835
MaeII ACGT 3 cut(s) 81, 1054, 1607
MaeIII GTNAC 9 cut(s) 163, 404, 778, 1039, 1352, 1554, 1596, 2349, 2448
MalI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1126, 1794
MboI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
MboII GAAGA 7 cut(s) 100, 472, 960, 969, 1119, 1197, 1230
MfeI CAATTG 2 cut(s) 294, 1907
MflI RGATCY 4 cut(s) 182, 625, 818, 1124
MhlI GDGCHC 2 cut(s) 1277, 1618
MlsI TGGCCA 2 cut(s) 1166, 1756
MluCI AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2361, 2453
MluI ACGCGT 1 cut(s) 1093
MluNI TGGCCA 2 cut(s) 1166, 1756
MlyI GAGTC 2 cut(s) 1408, 1444
MmeI TCCRAC 3 cut(s) 197, 818, 977
Mox20I TGGCCA 2 cut(s) 1166, 1756
Mph1103I ATGCAT 1 cut(s) 1173
MscI TGGCCA 2 cut(s) 1166, 1756
MseI TTAA 5 cut(s) 119, 704, 849, 2016, 2521
MslI CAYNNNNRTG 1 cut(s) 1445
Msp20I TGGCCA 2 cut(s) 1166, 1756
MspI CCGG 5 cut(s) 192, 616, 694, 714, 2446
MspR9I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1392, 1521, 1977, 2502
MunI CAATTG 2 cut(s) 294, 1907
Mva1269I GAATGC 2 cut(s) 387, 1971
MvaI CCWGG 4 cut(s) 1392, 1521, 1977, 2502
MvnI CGCG 1 cut(s) 1095
NciI CCSGG 5 cut(s) 616, 617, 694, 695, 714
NdeII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
NlaIV GGNNCC 3 cut(s) 882, 1420, 1486
NmuCI GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2349, 2448
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 3 cut(s) 929, 1175, 1528
PaqCI CACCTGC 1 cut(s) 260
PasI CCCWGGG 1 cut(s) 1976
PciI ACATGT 1 cut(s) 925
PctI GAATGC 2 cut(s) 387, 1971
PfeI GAWTC 3 cut(s) 829, 1510, 1852
PflMI CCANNNNNTGG 2 cut(s) 980, 1147
PfoI TCCNGGA 1 cut(s) 712
PleI GAGTC 2 cut(s) 1407, 1444
PpsI GAGTC 2 cut(s) 1407, 1444
PpuMI RGGWCCY 1 cut(s) 1418
PscI ACATGT 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 1418
Psp6I CCWGG 4 cut(s) 1390, 1519, 1975, 2500
PspGI CCWGG 4 cut(s) 1390, 1519, 1975, 2500
PspN4I GGNNCC 3 cut(s) 882, 1420, 1486
PspPI GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2489
PspPPI RGGWCCY 1 cut(s) 1418
PstI CTGCAG 5 cut(s) 1623, 2136, 2181, 2376, 2385
PstNI CAGNNNCTG 3 cut(s) 845, 1733, 2380
PsuI RGATCY 4 cut(s) 182, 625, 818, 1124
RsaI GTAC 5 cut(s) 204, 721, 745, 1303, 1486
RsaNI GTAC 5 cut(s) 203, 720, 744, 1302, 1485
RseI CAYNNNNRTG 1 cut(s) 1445
SaqAI TTAA 5 cut(s) 119, 704, 849, 2016, 2521
Sau3AI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
Sau96I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2489
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 1408, 1444
ScrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1392, 1521, 1977, 2502
SduI GDGCHC 2 cut(s) 1277, 1618
SfcI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2132, 2177, 2372, 2381
SinI GGWCC 2 cut(s) 911, 1418
SmaI CCCGGG 2 cut(s) 617, 695
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 1 cut(s) 1086
SmoI CTYRAG 1 cut(s) 1086
SrfI GCCCGGGC 1 cut(s) 695
Sse9I AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2361, 2453
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 402, 1701, 1835
StyD4I CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1390, 1519, 1975, 2500
StyI CCWWGG 4 cut(s) 943, 1330, 1360, 1471
TaaI ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2406
TaiI ACGT 3 cut(s) 84, 1057, 1610
TaqI TCGA 2 cut(s) 84, 1190
TasI AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2361, 2453
TatI WGTACW 2 cut(s) 719, 743
TauI GCSGC 6 cut(s) 44, 623, 1112, 1956, 2074, 2104
TfiI GAWTC 3 cut(s) 829, 1510, 1852
Tru1I TTAA 5 cut(s) 119, 704, 849, 2016, 2521
Tru9I TTAA 5 cut(s) 119, 704, 849, 2016, 2521
TscAI CASTG 4 cut(s) 539, 783, 876, 895
TseFI GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2349, 2448
Tsp45I GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2349, 2448
TspDTI ATGAA 8 cut(s) 842, 1115, 1224, 1278, 1467, 1814, 1865, 2466
TspGWI ACGGA 3 cut(s) 20, 223, 799
TspMI CCCGGG 2 cut(s) 615, 693
TspRI CASTG 4 cut(s) 539, 783, 876, 895
Van91I CCANNNNNTGG 2 cut(s) 980, 1147
VpaK11BI GGWCC 2 cut(s) 911, 1418
XagI CCTNNNNNAGG 1 cut(s) 942
XapI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2361
XceI RCATGY 3 cut(s) 929, 1175, 1528
XcmI CCANNNNNNNNNTGG 1 cut(s) 1388
XmaI CCCGGG 2 cut(s) 615, 693
XmiI GTMKAC 1 cut(s) 1987
XspI CTAG 3 cut(s) 402, 1701, 1835
ZraI GACGTC 1 cut(s) 1608
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.