Rh4AG427700

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000223
Physical Location & Seq
Forward (+)
72373189 .. 72378730
5542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG427700.1

Sequence Viewer

Length: 4308 bp
ATGGATGATTTTGATGATGAGGAAGCTCAATTTAGGGGTATCCCAGATTCTTTTTGTGATATTCCTCCAAAATTTTATCCTCTTTTCGTAACATTTCATAAGTTCTTGATGATGCTTGATGGAAGTCTGAGTAATTCATACTTTGAAAGATTCCCAGACATCGCAAAACTACCTCAAGATCGACAGCGAATATCAAGAGCTAATGTGCTGCAGACCTTCCTCACCACCAAGGAGGTCACATATGCAAGGTTTAGCTCATCATATTGGCCTCATTTTGATGTTCAGTTGACAAAGAAGCTTGATGCTTCACGAGTCTTTACTGAGATTATTTCTCATATCAAAGGTGGTCTTAGAGCGTTGGAAGCAGGTGATGGAAAACTCAGTCAGTTGGATTATGTGCAAATGTCAGAGGGCCGGGCTTCCAGTTTAAGCCAGCAAAAGAGAGAGATAATATATGATATATTTCAGGTATATGAGAAAATGAAAATGAGAAATGGTGAGTTTGATATTGCTGATTTTGTAAATGATATTCACCATCGGCTCAAATGTGAAAAATACAAGGGTGATGAAATTGATTTTGTGTACATTGATGAGGTCCAGGATCTTGCCATGAGCCAAATTGCATTGTTTAAACATATCTGCAGCAATGTTGAAGAGGGTTTCGTTTTTTCTGGAGATACAGCCCAGACTATAGCAAGGGGTATTGATTTTAGATTCCAAGATATACGACATCTCTTTTACAAGAAGTTTGTATTGGAATCAAGAAAAAAAAAACACGACAATAGGAAAGAAAAAGGACAGATCTCAAACATCTTTCAGTTGACACAAAACTTCCGAAGCCATGATGGGATACTGAAGTTGTTGCAGAGCATTGTTGAACTGATTTATCATTTTTTCCCCCTCTGTATTGATGTCTTAGAACCAGAAACGAGTATGATATATGGGGAAGCTCCGATTCTACTCTATTCTGGAAATGATGAAAAACTATTCAAAAAAATTCTAGGAAGTAGTGGGATTATTGCCGGAAATATGGGTGGCTTTGGTGCGGATCAAGTTATTTTGGTCCGTGATGATTGTTCTCGAGAAGAAATCTCTAACTCCATTGGGAAGCAAGCTCTAGTTCTTACTATAGTGGAGTGCAAGGGCCTAGAATTTGAGGATGTACTCCTATACAAATTTTTTGGATCATCACCACTGAAAAATCAATGGAGGGTGCTATATGAATTCATGCAAGAACAAAATTTGCTGGACTCCACATTACCTGAGCGCTTTCCAAGTTTTGATGATGCCAAACACAGCATGTTATGCTCTGAACTAAAGCAGTTATATGTGGCTGTCTCTCGTACAAGACAGAGACTCTGGATATACGAGAATGTTGAAGAACTTTCCAACCCAATTTTTGACTATTGGAAAAGGCAACATCTTGTACAACTTAGAGAACTAGACGATTCACTTGCACAAATTATGCAAGTTGCAAGCACTTCAGGAGACTGGAGATCACGTGGCATCAAGCTATATCATGAGCATAACTACAAAATGGCCAGAATGTGCTTTCATAAAGCTGGTGATACATTTTGGGAAAGACGGTCTGAAGCTGCTGAGCTTAAAGTCAAGGCAGACCATATGCGCACTTCTAGTCCTGGAGAGGCAAATGCACTCCTTAGGAAAGCAGCTTCAATTTTTGAAGCTATAGGCCTGTTTGTACCTTCTGCCAGATGCTTTTATGATTTGGGCGAGTATGAACGAGCAGGAACAATTTATTTGTATAAATGTGGCGAACTGGAACTGGAAAATGCTGGGGAGTGCTTTTATCTTGCTGAAAATTATGTGCTTGCTGCAGATGTGTATGCTAGAGGAAACTTTTTCTTTCTGAGTATTTCTTCTTGTTTACGAGGAAATATATTTCACAGAGGTTTGGAATACATAAAATATTGGAGACAACATGCAAGAGACGAGTATGATCTGGCCAGACAAGCAGATGAGACTCATGAAATGGAATTGGAATTTCTTGAGCATTGCGCATTTCACTATTATGAGGGAAAAGATGACAGATCAAGGATGAAAGTTCTTAGAGATGGAATGCTTTCTACTCGAAAGTTTTTGAATGCTCAGATTTCATCACGCACTTCAAAATATTTGTGGGAAGAAAAACTGCCTGGTGATCTTAAAAGGCCTTCAAACAAAAAATATGAGAAGCAGGTTTCTATTGATTCACTGGTCTACTTCTGGAATTCTTGGAAGGATACAGTTGTACACCTGTTTGAATATCTTGGATCGATAGATGTTAATGATTACATAAGTCATGGTGACTTCTTTCTGAATTATTTAGGGGTGTGGAGGCATTTTCATGATGGTCTAAATCCGCTCTATCTTTCACTCATTTATGATATTGACTGGGTGAGAAGTATTGACAAGAGATCTTTCCCAAGAAATGAGGAGTCAATCCCCATTGATGTTCACCGACTTGTCTTGGATGCTCAGAGTTATTGGAGTTCAGAGATGCTTTCTCTTGGGATTAAGGTTTTGGAGAAGCTTGAAGTGCTTTACAACTTCGCAATCAATAATTCTGATTCAGTGTTCTGCCAGAGCCGGTCTCTTATCCTTATATACGAGGTTGCAAAATATCTTCTGGAATCCAAATTTCTGAAGATGACTCATTATCATGCTGAGACACTCCACAGATTTGTTACATTGTCGACTGAGAATTTTGTCTCTTACATATTTCCTTTAGATTGGATGAGGTCATCAAGAGAGGATATGATTTCTCTTAGGCAAATTGATTCTTGTAAGTGTTTACTGAATCAAGTCATAGTTGAGTACATCAGCTCGACAAACAGGCTTTCATCTGGGCAAATGGGATGTATTGCGATGATCATTCTTGGGTCCGGTAAGCTGGATAATGAACTTTATAAGAAGCTTATAAAAAATCTAGACTGCAACTCTCCATGGAAAAAGTTCATTGAGGATCTATGTGACAGTATTCAGTCAGAGCTGCCAGAAGGTTATAATAGTGAGCGCCCTAAAAATGCCACAGTTCTTGAATATTCGGTGGCACCTGAGAGAGGTCCTTTACTTGGAAATATCAGTCATCAAGAACCGAGAGAGGTGTCTCTTTTGTGGAAGTTCCATGGTGCTTTGTTCAAAACTTATAGTCAGAATTGGAGTTTACATTGCTACAATATAACACCTGATTGTTTCTTATATCTTGTAGATTGCCTTCTGATTCGGATCTGTTGCTTTCAAGGTTATGCAATCACTACAAGATCTTGTTTCATTGAGCGGCTGATATATAAGGAGGAACATAATCAAGTGATAGTGGATGATGTTCGAATATCTTTTGAATGCATCCTTCAGTTTCTGACTGATGTTGTTCGGGAATTTCTTTTTAATAAGACGGATATGATTAAGTGGATAAAAAAGTGTACCAATAATAGTTGGAAGGTGTATTACTCGCTACTGATGCAGAGATTGATTTTTGTGTTATGTCTGCTTTATTTGAATTTTGGGATGGGCTTTGATATTCTCTTGGATTTGCTGAGGAGGAAGTACATCACTGACCAGTTACCAAAGGAGTTCTGCTATGTCCTTAGGGGAATTGCATCTTTACGTGATTCTGTTAGTAGACATGTTGATGTGCTAGCTCGAGCTTTTAAGAAGAGCGGTAATCCTTTGGTGGTTGCAAGTTTTGGGATTGATTGTTCAAGGTTCTTCTGTTCAGATGCCATTATCATCAACATGAAGGCTAACATATGCATGGCTGAAATAGTAAGATCTCTATTTCCAAAACACTCTATGATTCGTTCTTCACAAGGGCAGACATACACCACTGAGAACCTTCGGCATTGGAACAGAAATAACTTCCCCGTGGATTTTTATCCCTTGTGGGAAATATTTAAAGGTTTTAAACCAGCAAAGGAAAGAGATCAGTGCAATATATTTTCAGATGCCTCAAAAATCAAGTTGGATGTGGAGAAGATTATTCGGCTTTTGGCTGCCGCATGGTCTGGCCTTGATGACAAAGAAGAACGAAAGTTATCTAGAGAAGTGCTGAGCATGCTGGATGAATTGGTGCAACTCTATGCCGCATTAGATGGGAGCGAACGTGAAGTTGGAAACAATATGGCTACAGTTGCAGAACTTTCCAGGAGGATGCAGTGGAGAAGTCAAAGATTTTTTTGGCCGGAAAAAATTCAGCCCATCTTCACTGAACTGTATATGGAGCACAATAATAAACTAGCTAGGAAGGCACATAAGGCTGTTGCCAAGTCTGGCCACGGCAATGAGGCAGGAAACGGTGATCGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1435

Amino Acids

167.46

Weight (kDa)

6.44

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 142 - 231 1.3e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2910, 2921, 3006
AarI CACCTGC 1 cut(s) 356
Acc16I TGCGCA 2 cut(s) 1628, 2020
Acc36I ACCTGC 2 cut(s) 356, 2188
AccB1I GGYRCC 1 cut(s) 3052
AccBSI CCGCTC 3 cut(s) 2365, 3280, 3660
AccI GTMKAC 3 cut(s) 2220, 2696, 3622
AciI CCGC 6 cut(s) 1046, 2363, 3280, 3660, 3996, 4083
AclWI GGATC 6 cut(s) 609, 1056, 1192, 2281, 2973, 3236
AcoI YGGCCR 4 cut(s) 1539, 1965, 4178, 4270
AcuI CTGAAG 5 cut(s) 875, 1467, 1611, 2666, 3335
AcvI CACGTG 1 cut(s) 1502
AfaI GTAC 9 cut(s) 584, 1164, 1345, 1428, 1704, 2253, 2819, 3424, 3548
AfeI AGCGCT 1 cut(s) 1268
AfiI CCNNNNNNNGG 2 cut(s) 3062, 3074
AflIII ACRYGT 1 cut(s) 3625
AjnI CCWGG 4 cut(s) 597, 1639, 2155, 4142
AjuI GAANNNNNNNTTGG 4 cut(s) 737, 769, 4092, 4124
AloI GAACNNNNNNTCC 2 cut(s) 3682, 3714
Alw21I GWGCWC 1 cut(s) 4224
AlwI GGATC 6 cut(s) 609, 1056, 1192, 2281, 2973, 3236
AlwNI CAGNNNCTG 1 cut(s) 3358
Ama87I CYCGRG 2 cut(s) 1080, 3642
Aor51HI AGCGCT 1 cut(s) 1268
ApeKI GCWGC 7 cut(s) 208, 642, 1595, 1670, 1835, 2992, 3992
Asp700I GAANNNNTTC 3 cut(s) 2083, 2954, 4188
AspLEI GCGC 4 cut(s) 1269, 1629, 2021, 3018
AspS9I GGNCC 6 cut(s) 412, 595, 1063, 1144, 2883, 3065
AsuC2I CCSGG 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 3328
AsuNHI GCTAGC 1 cut(s) 3637
AvaI CYCGRG 2 cut(s) 1080, 3642
AvaII GGWCC 4 cut(s) 595, 1063, 2883, 3065
AxyI CCTNAGG 2 cut(s) 1661, 3587
BaeI ACNNNNGTAYC 4 cut(s) 842, 875, 2235, 2268
BalI TGGCCA 3 cut(s) 1541, 1967, 4272
BanI GGYRCC 1 cut(s) 3052
BarI GAAGNNNNNNTAC 4 cut(s) 3201, 3233, 3431, 3463
BauI CACGAG 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 1502
Bbv12I GWGCWC 1 cut(s) 4224
BbvCI CCTCAGC 1 cut(s) 3536
BbvI GCAGC 7 cut(s) 195, 654, 1582, 1682, 1822, 2979, 3979
BccI CCATC 9 cut(s) 113, 365, 543, 839, 2069, 2345, 3502, 4085, 4205
BceAI ACGGC 1 cut(s) 4291
BcgI CGANNNNNNTGC 2 cut(s) 1436, 1470
BciT130I CCWGG 4 cut(s) 599, 1641, 2157, 4144
BciVI GTATCC 3 cut(s) 50, 843, 2236
BclI TGATCA 1 cut(s) 2871
BcnI CCSGG 1 cut(s) 416
BfmI CTRYAG 7 cut(s) 209, 640, 690, 1128, 1689, 1836, 4125
BfoI RGCGCY 2 cut(s) 1270, 3019
BfuAI ACCTGC 2 cut(s) 356, 2188
BfuI GTATCC 3 cut(s) 50, 843, 2236
BglII AGATCT 4 cut(s) 801, 2417, 3263, 3770
BlpI GCTNAGC 2 cut(s) 1599, 4049
Bme1390I CCNGG 5 cut(s) 416, 599, 1641, 2157, 4144
Bme18I GGWCC 4 cut(s) 595, 1063, 2883, 3065
BmeT110I CYCGRG 2 cut(s) 1080, 3642
BmgT120I GGNCC 6 cut(s) 412, 595, 1063, 1144, 2883, 3065
BmiI GGNNCC 2 cut(s) 2884, 3054
BmrFI CCNGG 5 cut(s) 416, 599, 1641, 2157, 4144
BmrI ACTGGG 1 cut(s) 2404
BmtI GCTAGC 1 cut(s) 3641
BmuI ACTGGG 1 cut(s) 2404
BplI GAGNNNNNCTC 2 cut(s) 2578, 2610
BpmI CTGGAG 3 cut(s) 693, 1513, 1662
Bpu10I CCTNAGC 2 cut(s) 1263, 3536
Bpu1102I GCTNAGC 2 cut(s) 1599, 4049
Bpu14I TTCGAA 1 cut(s) 3328
BpuEI CTTGAG 2 cut(s) 159, 2030
BpuMI CCSGG 1 cut(s) 416
Bsa29I ATCGAT 1 cut(s) 2276
BsaAI YACGTR 2 cut(s) 1502, 3608
BsaBI GATNNNNATC 2 cut(s) 3227, 3873
BsaI GGTCTC 1 cut(s) 2598
BsaJI CCNNGG 5 cut(s) 228, 2945, 3127, 3864, 4273
BsaWI WCCGGW 1 cut(s) 2885
BsaXI ACNNNNNCTCC 4 cut(s) 1486, 1516, 4087, 4117
Bsc4I CCNNNNNNNGG 2 cut(s) 3062, 3074
Bse118I RCCGGY 1 cut(s) 2589
Bse1I ACTGG 7 cut(s) 423, 1496, 1785, 1791, 2220, 2399, 3559
Bse21I CCTNAGG 2 cut(s) 1661, 3587
Bse3DI GCAATG 4 cut(s) 652, 2014, 3169, 4285
Bse8I GATNNNNATC 2 cut(s) 3227, 3873
BseBI CCWGG 4 cut(s) 599, 1641, 2157, 4144
BseCI ATCGAT 1 cut(s) 2276
BseDI CCNNGG 5 cut(s) 228, 2945, 3127, 3864, 4273
BseJI GATNNNNATC 2 cut(s) 3227, 3873
BseLI CCNNNNNNNGG 2 cut(s) 3062, 3074
BseMI GCAATG 4 cut(s) 652, 2014, 3169, 4285
BseNI ACTGG 7 cut(s) 423, 1496, 1785, 1791, 2220, 2399, 3559
BseRI GAGGAG 2 cut(s) 2450, 3553
BseXI GCAGC 7 cut(s) 195, 654, 1582, 1682, 1822, 2979, 3979
BseYI CCCAGC 1 cut(s) 1796
BshNI GGYRCC 1 cut(s) 3052
BshVI ATCGAT 1 cut(s) 2276
BsiHKAI GWGCWC 1 cut(s) 4224
BsiHKCI CYCGRG 2 cut(s) 1080, 3642
BsiSI CCGG 5 cut(s) 415, 1023, 2590, 2886, 4181
BslI CCNNNNNNNGG 2 cut(s) 3062, 3074
BsmBI CGTCTC 1 cut(s) 1944
BsmI GAATGC 3 cut(s) 2085, 2110, 3347
Bso31I GGTCTC 1 cut(s) 2598
BsoBI CYCGRG 2 cut(s) 1080, 3642
Bsp119I TTCGAA 1 cut(s) 3328
Bsp1286I GDGCHC 1 cut(s) 4224
Bsp1407I TGTACA 3 cut(s) 582, 1426, 2251
Bsp1720I GCTNAGC 2 cut(s) 1599, 4049
Bsp19I CCATGG 2 cut(s) 2945, 3127
BspACI CCGC 6 cut(s) 1046, 2363, 3280, 3660, 3996, 4083
BspDI ATCGAT 1 cut(s) 2276
BspHI TCATGA 3 cut(s) 1519, 1987, 2347
BspLI GGNNCC 2 cut(s) 2884, 3054
BspMAI CTGCAG 3 cut(s) 213, 644, 1840
BspMI ACCTGC 2 cut(s) 356, 2188
BspOI GCTAGC 1 cut(s) 3641
BspPI GGATC 6 cut(s) 609, 1056, 1192, 2281, 2973, 3236
BspQI GCTCTTC 1 cut(s) 3650
BspT104I TTCGAA 1 cut(s) 3328
BspT107I GGYRCC 1 cut(s) 3052
BspTNI GGTCTC 1 cut(s) 2598
BsrBI CCGCTC 3 cut(s) 2365, 3280, 3660
BsrDI GCAATG 4 cut(s) 652, 2014, 3169, 4285
BsrFI RCCGGY 1 cut(s) 2589
BsrGI TGTACA 3 cut(s) 582, 1426, 2251
BsrI ACTGG 7 cut(s) 423, 1496, 1785, 1791, 2220, 2399, 3559
BssAI RCCGGY 1 cut(s) 2589
BssECI CCNNGG 5 cut(s) 228, 2945, 3127, 3864, 4273
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 3 cut(s) 228, 2945, 3127
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 4 cut(s) 599, 1641, 2157, 4144
Bst4CI ACNGT 8 cut(s) 1587, 2248, 2978, 3034, 4129, 4212, 4295, 4305
Bst6I CTCTTC 2 cut(s) 648, 3650
BstAPI GCANNNNNTGC 1 cut(s) 1305
BstAUI TGTACA 3 cut(s) 582, 1426, 2251
BstBAI YACGTR 2 cut(s) 1502, 3608
BstBI TTCGAA 1 cut(s) 3328
BstC8I GCNNGC 6 cut(s) 434, 1113, 1477, 1833, 3639, 4055
BstDSI CCRYGG 4 cut(s) 2945, 3127, 3864, 4273
BstENI CCTNNNNNAGG 1 cut(s) 3060
BstH2I RGCGCY 2 cut(s) 1270, 3019
BstHHI GCGC 4 cut(s) 1269, 1629, 2021, 3018
BstMWI GCNNNNNNNGC 9 cut(s) 362, 1305, 1973, 2539, 3460, 4054, 4130, 4244, 4253
BstNI CCWGG 4 cut(s) 599, 1641, 2157, 4144
BstNSI RCATGY 4 cut(s) 1303, 1946, 3629, 4057
BstSCI CCNGG 5 cut(s) 414, 597, 1639, 2155, 4142
BstSFI CTRYAG 7 cut(s) 209, 640, 690, 1128, 1689, 1836, 4125
BstV1I GCAGC 7 cut(s) 195, 654, 1582, 1682, 1822, 2979, 3979
BstX2I RGATCY 7 cut(s) 601, 801, 2417, 2965, 3228, 3263, 3770
BstYI RGATCY 7 cut(s) 601, 801, 2417, 2965, 3228, 3263, 3770
Bsu15I ATCGAT 1 cut(s) 2276
Bsu36I CCTNAGG 2 cut(s) 1661, 3587
BsuI GTATCC 3 cut(s) 50, 843, 2236
BsuTUI ATCGAT 1 cut(s) 2276
BtgI CCRYGG 4 cut(s) 2945, 3127, 3864, 4273
BtgZI GCGATG 2 cut(s) 145, 2882
BtsI GCAGTG 1 cut(s) 4160
BtsIMutI CAGTG 8 cut(s) 1193, 2213, 2580, 3552, 3825, 3932, 4160, 4203
BveI ACCTGC 2 cut(s) 356, 2188
Cac8I GCNNGC 6 cut(s) 434, 1113, 1477, 1833, 3639, 4055
CaiI CAGNNNCTG 1 cut(s) 3358
CciI TCATGA 3 cut(s) 1519, 1987, 2347
CfoI GCGC 4 cut(s) 1269, 1629, 2021, 3018
Cfr10I RCCGGY 1 cut(s) 2589
Cfr13I GGNCC 6 cut(s) 412, 595, 1063, 1144, 2883, 3065
ClaI ATCGAT 1 cut(s) 2276
Csp6I GTAC 9 cut(s) 583, 1163, 1344, 1427, 1703, 2252, 2818, 3423, 3547
CspCI CAANNNNNGTGG 2 cut(s) 3019, 3054
CviQI GTAC 9 cut(s) 583, 1163, 1344, 1427, 1703, 2252, 2818, 3423, 3547
DraI TTTAAA 3 cut(s) 631, 3895, 3904
EaeI YGGCCR 4 cut(s) 1539, 1965, 4178, 4270
Eam1104I CTCTTC 2 cut(s) 648, 3650
EarI CTCTTC 2 cut(s) 648, 3650
Eco130I CCWWGG 3 cut(s) 228, 2945, 3127
Eco147I AGGCCT 2 cut(s) 1695, 2173
Eco31I GGTCTC 1 cut(s) 2598
Eco47I GGWCC 4 cut(s) 595, 1063, 2883, 3065
Eco47III AGCGCT 1 cut(s) 1268
Eco57I CTGAAG 5 cut(s) 875, 1467, 1611, 2666, 3335
Eco72I CACGTG 1 cut(s) 1502
Eco81I CCTNAGG 2 cut(s) 1661, 3587
Eco88I CYCGRG 2 cut(s) 1080, 3642
EcoNI CCTNNNNNAGG 1 cut(s) 3060
EcoO109I RGGNCCY 2 cut(s) 1144, 3065
EcoRI GAATTC 2 cut(s) 1223, 2230
EcoRII CCWGG 4 cut(s) 597, 1639, 2155, 4142
EcoT14I CCWWGG 3 cut(s) 228, 2945, 3127
EcoT22I ATGCAT 2 cut(s) 3347, 3755
ErhI CCWWGG 3 cut(s) 228, 2945, 3127
Esp3I CGTCTC 1 cut(s) 1944
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FauNDI CATATG 3 cut(s) 241, 1623, 3749
FbaI TGATCA 1 cut(s) 2871
FblI GTMKAC 3 cut(s) 2220, 2696, 3622
FspAI RTGCGCAY 1 cut(s) 1628
FspI TGCGCA 2 cut(s) 1628, 2020
GlaI GCGC 4 cut(s) 1268, 1628, 2020, 3017
GsaI CCCAGC 1 cut(s) 1800
GsuI CTGGAG 3 cut(s) 693, 1513, 1662
HaeII RGCGCY 2 cut(s) 1270, 3019
HapII CCGG 5 cut(s) 415, 1023, 2590, 2886, 4181
HhaI GCGC 4 cut(s) 1269, 1629, 2021, 3018
Hin6I GCGC 4 cut(s) 1267, 1627, 2019, 3016
HinP1I GCGC 4 cut(s) 1267, 1627, 2019, 3016
HincII GTYRAC 3 cut(s) 288, 822, 2697
HindII GTYRAC 3 cut(s) 288, 822, 2697
HindIII AAGCTT 3 cut(s) 296, 2531, 2915
HpaII CCGG 5 cut(s) 415, 1023, 2590, 2886, 4181
HpyCH4III ACNGT 8 cut(s) 1587, 2248, 2978, 3034, 4129, 4212, 4295, 4305
HpyCH4IV ACGT 3 cut(s) 1501, 3607, 4102
HpyF10VI GCNNNNNNNGC 9 cut(s) 362, 1305, 1973, 2539, 3460, 4054, 4130, 4244, 4253
HpySE526I ACGT 3 cut(s) 1501, 3607, 4102
HspAI GCGC 4 cut(s) 1267, 1627, 2019, 3016
Ksp22I TGATCA 1 cut(s) 2871
LguI GCTCTTC 1 cut(s) 3650
LmnI GCTCC 3 cut(s) 955, 4095, 4219
Lsp1109I GCAGC 7 cut(s) 195, 654, 1582, 1682, 1822, 2979, 3979
MaeII ACGT 3 cut(s) 1501, 3607, 4102
MaeIII GTNAC 6 cut(s) 88, 235, 2306, 2686, 2972, 3561
MbiI CCGCTC 3 cut(s) 2365, 3280, 3660
MflI RGATCY 7 cut(s) 601, 801, 2417, 2965, 3228, 3263, 3770
MhlI GDGCHC 1 cut(s) 4224
MlsI TGGCCA 3 cut(s) 1541, 1967, 4272
MluNI TGGCCA 3 cut(s) 1541, 1967, 4272
MlyI GAGTC 6 cut(s) 321, 1244, 1350, 1978, 2447, 2647
MmeI TCCRAC 6 cut(s) 339, 369, 1413, 3416, 3942, 4090
Mox20I TGGCCA 3 cut(s) 1541, 1967, 4272
Mph1103I ATGCAT 2 cut(s) 3347, 3755
MroXI GAANNNNTTC 3 cut(s) 2083, 2954, 4188
MscI TGGCCA 3 cut(s) 1541, 1967, 4272
MslI CAYNNNNRTG 8 cut(s) 276, 2031, 2346, 2661, 3630, 3734, 3752, 4278
Msp20I TGGCCA 3 cut(s) 1541, 1967, 4272
MspI CCGG 5 cut(s) 415, 1023, 2590, 2886, 4181
MspR9I CCNGG 5 cut(s) 416, 599, 1641, 2157, 4144
MssI GTTTAAAC 1 cut(s) 631
Mva1269I GAATGC 3 cut(s) 2085, 2110, 3347
MvaI CCWGG 4 cut(s) 599, 1641, 2157, 4144
MwoI GCNNNNNNNGC 9 cut(s) 362, 1305, 1973, 2539, 3460, 4054, 4130, 4244, 4253
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 2 cut(s) 2945, 3127
NdeI CATATG 3 cut(s) 241, 1623, 3749
NheI GCTAGC 1 cut(s) 3637
NlaIV GGNNCC 2 cut(s) 2884, 3054
NmuCI GTSAC 3 cut(s) 235, 2306, 2972
NsbI TGCGCA 2 cut(s) 1628, 2020
NsiI ATGCAT 2 cut(s) 3347, 3755
NspI RCATGY 4 cut(s) 1303, 1946, 3629, 4057
NspV TTCGAA 1 cut(s) 3328
PaeI GCATGC 1 cut(s) 4057
PaeR7I CTCGAG 2 cut(s) 1080, 3642
PagI TCATGA 3 cut(s) 1519, 1987, 2347
PaqCI CACCTGC 1 cut(s) 356
PceI AGGCCT 2 cut(s) 1695, 2173
PciI ACATGT 1 cut(s) 3625
PciSI GCTCTTC 1 cut(s) 3650
PctI GAATGC 3 cut(s) 2085, 2110, 3347
PdmI GAANNNNTTC 3 cut(s) 2083, 2954, 4188
PfoI TCCNGGA 3 cut(s) 597, 1639, 4142
PleI GAGTC 6 cut(s) 320, 1244, 1350, 1978, 2446, 2647
PmaCI CACGTG 1 cut(s) 1502
PmeI GTTTAAAC 1 cut(s) 631
PmlI CACGTG 1 cut(s) 1502
PpsI GAGTC 6 cut(s) 320, 1244, 1350, 1978, 2446, 2647
Ppu21I YACGTR 2 cut(s) 1502, 3608
PpuMI RGGWCCY 1 cut(s) 3065
PscI ACATGT 1 cut(s) 3625
PsiI TTATAA 3 cut(s) 2910, 2921, 3006
Psp5II RGGWCCY 1 cut(s) 3065
Psp6I CCWGG 4 cut(s) 597, 1639, 2155, 4142
PspCI CACGTG 1 cut(s) 1502
PspFI CCCAGC 1 cut(s) 1796
PspGI CCWGG 4 cut(s) 597, 1639, 2155, 4142
PspN4I GGNNCC 2 cut(s) 2884, 3054
PspPI GGNCC 6 cut(s) 412, 595, 1063, 1144, 2883, 3065
PspPPI RGGWCCY 1 cut(s) 3065
PspXI VCTCGAGB 1 cut(s) 3642
PstI CTGCAG 3 cut(s) 213, 644, 1840
PstNI CAGNNNCTG 1 cut(s) 3358
PsuI RGATCY 7 cut(s) 601, 801, 2417, 2965, 3228, 3263, 3770
RsaI GTAC 9 cut(s) 584, 1164, 1345, 1428, 1704, 2253, 2819, 3424, 3548
RsaNI GTAC 9 cut(s) 583, 1163, 1344, 1427, 1703, 2252, 2818, 3423, 3547
RseI CAYNNNNRTG 8 cut(s) 276, 2031, 2346, 2661, 3630, 3734, 3752, 4278
SalI GTCGAC 1 cut(s) 2695
SapI GCTCTTC 1 cut(s) 3650
Sau96I GGNCC 6 cut(s) 412, 595, 1063, 1144, 2883, 3065
SchI GAGTC 6 cut(s) 321, 1244, 1350, 1978, 2447, 2647
ScrFI CCNGG 5 cut(s) 416, 599, 1641, 2157, 4144
SduI GDGCHC 1 cut(s) 4224
SfcI CTRYAG 7 cut(s) 209, 640, 690, 1128, 1689, 1836, 4125
Sfr274I CTCGAG 2 cut(s) 1080, 3642
SfuI TTCGAA 1 cut(s) 3328
SinI GGWCC 4 cut(s) 595, 1063, 2883, 3065
SlaI CTCGAG 2 cut(s) 1080, 3642
SmiMI CAYNNNNRTG 8 cut(s) 276, 2031, 2346, 2661, 3630, 3734, 3752, 4278
SmlI CTYRAG 4 cut(s) 174, 1080, 2009, 3642
SmoI CTYRAG 4 cut(s) 174, 1080, 2009, 3642
SphI GCATGC 1 cut(s) 4057
SseBI AGGCCT 2 cut(s) 1695, 2173
SsiI CCGC 6 cut(s) 1046, 2363, 3280, 3660, 3996, 4083
SspI AATATT 4 cut(s) 1931, 2135, 3044, 3891
StuI AGGCCT 2 cut(s) 1695, 2173
StyD4I CCNGG 5 cut(s) 414, 597, 1639, 2155, 4142
StyI CCWWGG 3 cut(s) 228, 2945, 3127
TaaI ACNGT 8 cut(s) 1587, 2248, 2978, 3034, 4129, 4212, 4295, 4305
TaiI ACGT 3 cut(s) 1504, 3610, 4105
TaqI TCGA 9 cut(s) 181, 1081, 2092, 2276, 2696, 2828, 3328, 3643, 4300
TatI WGTACW 6 cut(s) 582, 1162, 1426, 2251, 2817, 3546
TauI GCSGC 3 cut(s) 3283, 3998, 4085
TscAI CASTG 8 cut(s) 1200, 2220, 2580, 3559, 3832, 3932, 4160, 4210
TseFI GTSAC 3 cut(s) 235, 2306, 2972
TseI GCWGC 7 cut(s) 208, 642, 1595, 1670, 1835, 2992, 3992
Tsp45I GTSAC 3 cut(s) 235, 2306, 2972
TspGWI ACGGA 2 cut(s) 1055, 3410
TspRI CASTG 8 cut(s) 1200, 2220, 2580, 3559, 3832, 3932, 4160, 4210
VpaK11BI GGWCC 4 cut(s) 595, 1063, 2883, 3065
XagI CCTNNNNNAGG 1 cut(s) 3060
XbaI TCTAGA 2 cut(s) 2929, 4037
XceI RCATGY 4 cut(s) 1303, 1946, 3629, 4057
XhoI CTCGAG 2 cut(s) 1080, 3642
XmiI GTMKAC 3 cut(s) 2220, 2696, 3622
XmnI GAANNNNTTC 3 cut(s) 2083, 2954, 4188
Zsp2I ATGCAT 2 cut(s) 3347, 3755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.