Rw4G036630

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000223
Physical Location & Seq
Forward (+)
63454947 .. 63492420
37474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036630.1

Sequence Viewer

Length: 7809 bp
ATGGAGGTTTCAGGCAAGAAAAAGATCAAGCCTGACCCTTTCACTGATACTGTGTTTTCTTGGTCCCTGGAGACATTTTCAATGAGGATCTTTACAAAGACAAGGTATTCGCTTCCACTTTTAATAGTGAAAAAGATTCCTGAATCATTTCAATCGGCTCAGCATTACTTTGGGTGTTATATATATCCTTTACTGGAAGAAACCCGAGCAGACTTGCATTCAAGTATGGAATCTCTTAATAGAGCACCGTTTGCTGAAGTAGTGGGTTTTGAAGAAGCCAAGCCATATGGAACAAAGCTATATGATGTCAAGGTTGATTACTGGCGAAACAGGTTTCGTGATAGTGGCAAGGAACCATATAAAACTTTGCCCGGTGATCTTTTTGTTTTAGCTGATGCTAAACCTGAAACTGTTTATGATTTACAAGGAGTAGTGGGGTCATGGGCTTTTGCATCCGCCACTAATGTCTCAGAAAGTGAGAATGAAGATGACAGTACCTCTCTTTGTTTCAAAGTCAAGGCATCAAAAGAGTTTGAAGTCGAAAATAGCAGGTCATCACTTTTTCTAGTTTTCTTGGTGAATTTAACCCGTGATGGAAGAATTTGGAAAGCGTTGCACATGTCCAGAAATCTGAAGATTATCAACGAAGTTCTATGCACTGATTCTATGGTTCAGAAAGATTTCTGCTCTGAAAGGAATAATGACATCTGGAATCAGAGTATAGTTGAGAGTTTTTCATCTGGATTGAATGAGTCCCAAACTGGGGCAGTGATGGCCTGCCTTGAAATGCTGCATTCTGGTGAAAAGTCTGCTGTGCAACTTATCTGGGGTCCTCCTGGTACTGGAAAAACAAAAACCACAGTTACTCTGCTTTCAACCCTGCTACAGATGAATCGTAAGACTCTTATTTGTGCTCCAACAAATGTTGCAATAACTGGAGTTGCTTCTCTTCTTGTGAAGAAGGTGAGTAAAGCAGAGCCAAATAATTTGTTTTGTTCTTTAGGAGAGATACTTTTATTTGGGAATAAGGAGCGACTCAAAATTGGTTCAGACATTGAAGAAATTTATATGGATTGCCGTGTCAAAAGGCTTGTAGATTGTTTGGGGCCAAGGGCTGGTTGGAGGCATTGCTTTGCTTCCATGATAGGTTTACTTGAGGATTGTGTTTCTGATTACCATATTTTCTTGGAAAATGAGTTCATGAACGACACAGAACAAAAGAATGTTAGTGAAACGAAAGATAAAGAATGCAGAAGAGGTGCTGAAGTTAAAAAGGAGAAGTGCAAAACATTTGTTCAATTTTTGAGAGATAGATTTGCGTCTACTGCAACGCCGTTAAGAAATTGCATTTCTGTGTTCTGTACTCATATAGGGAAAAATTATATTTCAGTAGACAAATTCCAAGACTTGGTTTTGCTTACCCAGTTAGTTAATTCCTTTGAGTTACTGTTGTCTCAACATAATGTTGTTCCTGAAGCGTTGGAAGAACTCTTTTCTCATTCGGTTGTTGAAGATGTGTCCGAGTCATTTGTAGGCAACTCCTTTGATCTTTGTATGATGAGAAGGGATTGCCTTTCAGCTTTACGCACTCTACAGGATTCTCTTAGTCAACTTAAACTTCCAAATATTAGGAATGAGGAATCTATACGGGAGTTCTGTTTTCAAAGAGCATCTCTAATATTTTGCACTGCATCTAGTTCATATAAGCTGCACAGAGTGGCAATGGAACCACTAAGCATTGTTGTTATTGACGAGGCTGCACAGCTAAAAGAGTGTGAGTCAACAATACCCCTGCAGCTTCCAGGTGTGAAGCATGCTGTTCTTGTTGGTGATGAGTGTCAGTTACCTGCTACGGTAAAAAGCAATGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTGAGCTTAATGGGTCATTCAAAACATATTTTAAATATGCAATACAGAATGCATCCTTCAATAAGTTTATTCCCAAATTCAAATTTCTATGATGACTTGATCCGGGATGCTCCAAATGTAAAACGAAGAAGCTACGAGAAGCACTATCTTCCAGGAGCAATGTTTGGTCCTTATTCTTTTATAAATGTGATTGGTGGAAGAGAGGAGAAAGATGAGGATGGACATAGTCGGAAAAATATGGTGGAGGTTGCTATTGTTTCAAAAATACTGCGGAATCTGTTCAAAGAATGGGTTGAATCAAAACAGAGACTCAGTATTGGTATAGTGTCTCCCTATGCTGCTCAAGTAGTCGCAATTAAGGACAGAGTGGGTAAGAAATATGATCAGCTTGATGGCTTTGAAGTGAAGGTAAAAACAGTTGATGGGTTTCAAGGTGGGGAAGAGGACATAATTATAATATCCACTGTACGATCCAACAGCCATCAATCACTTGAATTCATTTCAAAATCGCAAAGAGTTAATGTCTCTCTTACAAGGGCAAGGCACTGTTTGTGGATTTTGGGGAATGAAAGAACGCTTTCCGATGGTGAATCTGTTTGGAAGGCCTTGGTCCTTGATGCCAAAAGTCGTCAATGCTTCTTTAATGCCGATGAAGACAAGGATTTAGCCAAGGCCATATTAGAGGTCAAGAAAGAGTTTGACCAATTTGATGATTTGCTCAATGCCGACAGTGTTCTTTTCAGAAGTGCTAGATGGAAGGTACTTTTCAGTGATAACTTTCTGAAGTCATTCAAGAAACTGACATCAGTCCGCTTGAAGAAGTCAGTGCTAAATCTTCTACTGAAGCTTTCCAGTGGTTGGAGACCTAAGAAGCGAAATGTTGAGACAATTTCTGGTAGCTCTTGCATGATCTTGAAGAAATTTAAGGTTGAAGGTCTATACATTGTTTGCACTAATGACATTGCAAAGAACCTGCAGTACGTTCAAGTATTGAAGATCTGGGACTTATTGCCTCTAGAGGATATCCCAAAGTTGATAAACCGCCTGGATAGTATTTTCAACAGATATACTGATAACTTTATTAATCTTTGCAAGGAGAAGTGTCTCAATGGTGATCTAGAGGTTCCAAAGAGCTGGTCAACGTCTTTGGAATTTTCCCGGTTTAAGGATCTTAGCATCAGTGAAGCTCAGAGTGATTTAGTTGGTGACACTTCTGATTGTAGAAGCTATCTTGAGAATTCACAGGTTAGCGAGAGTTTGTTGCTGATGAAATTTTATTCCTTATCATCTGGGGTAGTAAACCACTTGCTGTCAGACCGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACAGACGAAGAGATGGAGATTATCCTTTATCGTAGAAGTTCCTTTATAGTGGGACGGTCAGGAACTGGGAAAACCACAGTTTTAACCATGAAACTGTTTCAAAAAGAACAATGGCACCAATTTGCGGTACAAGGATGCTATAGTAGTCAAAATACTAGCAAGGTTGAGCAGAGTTCTGCAGCTACTGAAGGGAAAGTTCTGCGCCAGCTTTTTGTGACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCGACATGTTTCACACTTGAAAAGTTTTGCATGCGGCGGAGGTAACTCAACTGACTCAACTGAAAGAGGTTTAATTGATATGGCTGATTTTGATGATGAGGAAACCCAATTCAAGGATATCCAAGATTCATTTCATGATATTCCTATGAGTTCTTACCCACTTGTTATTACATTCCATAAGTTTTTGATGATGCTCGATGGGACATTGAGTAACTCATACTTTGAAAGATTCCTTGATGTGACAGCTACTGTTGGTCATTTTAGGAGTTCAAGATCAGTCACATTTCAGAGCTTGATAAGGACGAAAGAAGTTAATTATGAGAGGTTTAGCTCTGTGTATTGGCCCCGTTTTAATACTCAGATAACAAAGAAGCTTGACATTTCAAGAGTCTTTATAGAGATTATTTCTTATATAAAAGGTGGCTTAGGAGCTATAGATGCATGTGATGGTAAACTCAGCCGGGAGGATTATGTTCAACTGTGCGAGGGCCGGGGTTCTAATTTGAGCAAGCAAAAGAGAGAAGCAATATACGATGTTTTTCAGGCTTATGAAAAGTTGAAGATGGAAAATGGTGAATTCGATCTGGCTGATTTTGTAATTGATCTTCACTGTAGACTCAAGCATGAAAAATATGAGGGTGATCAGATGGAATTTGTTTATATTGATGAGGTTCAAGATCTCACTATGAGTCAAATTGCTTTGTTTAAACACATGTGCAACAATGTTGAAGAGGGATTTGTCTTTTCTGGTGATACAGCACAAACAATAGCAAGGGGTATTGACTTCAGGTTTCAGGATATACGACATCTGTTCTACAAGAAGTTCGTATTGGAGTCAAGAAGCAATAACAATGATGAAAGAAAAGAAAAAGGCCTCATCTCTGAATTACTTCAGTTGACTCAAAACTTCCGTACTCATGCCGGTATATTGAAGTTATCACAAAGCATTGTTGAGCTACTGTATCGGTTTTTCCCCTTTTCTGTCGATGTGTTGAAACCTGAAACCAGTCTGATTTATGGGGAAGCTCCAGTTTTGCTTGAATCCGGAGAAAACGAAAATGCAATCATGAAAATTTTTGGAAACAGTGGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATTTTGGTGCGAGATGATAGTGCTCGACAAGAAATATCCAAATTTGTAGGGAAGCATGCTCTTGTCCTAACCATAGTGGAGTGTAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTCGGCACATCACCTTTGAAAAATCAGTGGAGGGTTATATATGATTACATGAAGGAACAAAATTTACTCGACTCCACATTACCTAAGCGCTTTCCAAGTTTCAGTGAATCAAAACACAACATATTGTGCTCTGAACTGAAGCAATTATATGTGGCTGTTACTCGAACAAGACAGAGATTGTGGATTTGTGAAAATGTGGAAGACCTCTCGAAACCTATGTTTGACTATTGGAAGAAAAAATGTCTTGTGCAACAAAGGCAGCTAGATGATTCACTTGCCCAGGCAATGCAAGTGGCAAGCAGTCCAGAGGAGTGGAAATCACGAGGCATTAAGCTATATCATGAACATAACTATGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGACACTTACTGGGAAAGGAGGTCTAAAGCCTCCGGCCTTAAAGCTATTGCTGACCGTATGCGAACATCAAATCCTGAAGAGGCTAAATCTATTCTTAGAGAAGCTGCTGAAATATTTGATGCTATTGGCAAGGCCGATTCTGCTGCGAGATGTTTTTCTGATTTGGGGGAGTATGAAAGAGCAGCTAGGATATATTTGGAAAAATGTGGAGACTCGGAACTTCAAGGAGCGGCAGAATGTTTTTCACTAGCGGGATGCTATAAGGATGCTGCAGATGTCTATGCCAAGGGAAATTTTTTCTCAGAGTGTCTCACTGTTTGTGCAAATGGGAAACTATTTGAAATGGGTTTGCAGTATATTAATTATTGGAAACAGCATGCTGTTGAGGACCGTGCTAAGGCTAGTACAGGGGAAGGAATAGATAAAAAGGAGCAGGAATTTTTAGAGAGCTGCGCACTTCACTATCATAAGTTGAAAGATAACAGATTCATGATGAAATTTGTGAAAGCTTTCAATTCTATAATATTGATGCGGGACTTTTTGAAGAATATAGGGAGCCTTGATGAGCTTTTGTCATTGGAGGAAGAATTTGGAAATTATCTGGAAGCCGCAAACACCGCCCAGCTTAAAGGTGAAATCCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAGGCCTCACTGCATATTCTGTTTTATGTAATTTCCAAGTCACTTTGGTCATCTGGTAGTAAGGGCTGGCCCATGAAAAAGTTTCCACAACAGGAGGAGCTTCTGACAAAGGCCAAGTCATTTGCCAAGAAAGGGAAAGATAACGTTTATGAGCTGGTTTGCGCTGAGTCAGAGATTTTATTGAATGGGCAGGATAACTTGGCTGTTTTGAAACATCAAATGGATGCTTCTGAAAGACATAAGAGTATTAGAGGTGAAGTGTTATCAGCTCGAAAAATTTTGGATGCCCATCTGTCTTCAAGAACTGATGAGTATTTATGGGAGAAAGAATTGATTGGAGATCTTGTAAAGCATTCAGAAGATAAAATATCTAGAAATCAGGTGTCCATTGATTCGCTTGCCTACTACTGGAATTTCTGGAAGGAGAAGATTCTTCACATCATTGAATCTGTAGGATGTGTGGAAACTCCGGGGTACAATAGTTATGTACAGTTCTGTTTTACTTGTTTAGGAGTTAGGAGGTTGTATCATAATCTGAATCCAATCTATGTTTTACGGGTCCCTGATGCTGATTGGGTTAGACATCTGGACAAAAGGCATTTGAAAAGTTATGGGAAGTTGGTCTCCATTGATGTTCATCAGCTCATTCTAGCAGCTCAGCATTATTGGCGTTCAGAACTGCTCTCTGTTGGCATCAAAGTTTTGGAAAAGCTTGAAGCACTTCCCAGGTTCCCACTAAAGAATTCTGACTCGACATTCTTCCAGTGCAGAGCTCTTACCCTTATCTATGATGTTGCAAGAAATCTTTTGGATTCCAAATGTCTGAAGCCAAGGTATCAAGATTGCTTGACACTACAGAATCTGGTAACATTTTCGACCGGGGAGTATGTTGATTACATCTTTCCCTTAGATTGGAGGAATTCATTGAGACAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATATGCCAGCTTGAAGGACGAGCTGTCTTATGGGCAGATTGGTAGGATCGCAATGATCATCCTCGGGTCAGGTAAGCTGCTGAATACTGAGCTCTGTGTTCAGCTTGTGAAAAAGCTTGAAATCAATAAACCATGGAAGGCCTTCATTGAGAATCTCTGTGCAAGCATTGGACCTGAACATACCCGTCAGGAACCAATAGAGGTGTCTGTCCTGTGGTGGCTACATGAGGCTTTGGTTGAAACTTATAGTGCCAACTGGAGGGAGGTTTCCGACTACATAACACCTGGTTGCTTCTTGTATCTTGTGGAGCGCCTTCTGTTTTCCGCAAGTTGCTCTCAAGGTTTTGTCATCACTACAAGTTCTTGCTTTATTGAATGGCTGATATACCAGGATGAAGATACCAGCTTACGTTCCATGGTGACTGGTGGGGGCCATTCTCTGGAAGACATTCTTCCATTTGTGATTACTGTGGTTCGCGAGTGTATTTTAAACAAGAAGGATATGATTGAATGGATTGAGAAGTCTGACAGAAATTGGAGGAAGTATTATCAGCCACTCATGTTGCGATTGGTTACTGTCTTGTGTTTGATTCATTTGAACTTTGGCTCGTGTTTGGATATGATCCATGATTTGCTGGGAAGTGACTTTATCACTGAACTCGTACCATGGGAGTTTTATGATGCACTTAGCAGGAGGAGGAAACCCAATTCTCTCATTTTAGATGTGAATGTCCTTGCTGGAGCTCTCAAGAAGATTGATAATATTATGGTGATTGCGAGTTTCCTCGGGCCTAATTGTTCAGGGTTTTACAGTTCCGATGCCATCTTCGTCGACATGACGGCCTACCAATGCAAGGATGACATATTGAGAAAATGGTTTCCAAATCCGACATGTCAACAAGATGTTCATACTTCCACCCCATCTGGAAGCAAGGCGAAGAAAAAGACAAAGGGCAAGAACAAGAAAAACAAGAAGAAAGGAGGCCGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2602

Amino Acids

297.44

Weight (kDa)

6.81

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 83 - 211 9.3e-61 Domain of unknown function (DUF6469)
AAA_11 PF13086 249 - 458 8e-16 AAA domain
AAA_11 PF13086 528 - 622 1.9e-18 AAA domain
AAA_12 PF13087 630 - 826 7.2e-56 AAA domain
UvrD-helicase PF00580 1337 - 1426 1.6e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2090, 2363
AarI CACCTGC 1 cut(s) 4615
Acc16I TGCGCA 1 cut(s) 5593
Acc36I ACCTGC 5 cut(s) 540, 1855, 2889, 4615, 5798
AccB1I GGYRCC 1 cut(s) 3375
AccB7I CCANNNNNTGG 4 cut(s) 1115, 1408, 2766, 7255
AccBSI CCGCTC 1 cut(s) 5369
AccI GTMKAC 4 cut(s) 1322, 1392, 4123, 7647
AccII CGCG 1 cut(s) 7293
AccIII TCCGGA 1 cut(s) 4553
AclI AACGTT 1 cut(s) 5964
AclWI GGATC 7 cut(s) 95, 2002, 2373, 3084, 3252, 6869, 7432
AcoI YGGCCR 1 cut(s) 5112
AdeI CACNNNGTG 2 cut(s) 1717, 5121
AfeI AGCGCT 1 cut(s) 4841
AflIII ACRYGT 4 cut(s) 618, 3514, 4221, 7706
AhdI GACNNNNNGTC 2 cut(s) 3483, 6838
AjnI CCWGG 9 cut(s) 66, 835, 1801, 2059, 2952, 5031, 6545, 7099, 7203
AjuI GAANNNNNNNTTGG 4 cut(s) 3624, 3656, 4322, 4354
AloI GAACNNNNNNTCC 4 cut(s) 1181, 1213, 5194, 5226
Alw21I GWGCWC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
AlwI GGATC 7 cut(s) 95, 2002, 2373, 3084, 3252, 6869, 7432
AlwNI CAGNNNCTG 4 cut(s) 3326, 3446, 3758, 6682
Ama87I CYCGRG 3 cut(s) 204, 6878, 7601
Aor13HI TCCGGA 1 cut(s) 4553
Aor51HI AGCGCT 1 cut(s) 4841
ArsI GACNNNNNNTTYG 6 cut(s) 91, 123, 3029, 3061, 5921, 5953
AseI ATTAAT 2 cut(s) 2991, 5499
AspLEI GCGC 5 cut(s) 3465, 4842, 5594, 5984, 7128
AsuC2I CCSGG 7 cut(s) 372, 2012, 3067, 3971, 4001, 6291, 6700
AvaI CYCGRG 3 cut(s) 204, 6878, 7601
AvaII GGWCC 7 cut(s) 63, 830, 2075, 2518, 5527, 6379, 6986
BaeI ACNNNNGTAYC 2 cut(s) 6286, 6319
BalI TGGCCA 1 cut(s) 5114
BanI GGYRCC 1 cut(s) 3375
BanII GRGCYC 3 cut(s) 6595, 6909, 7561
BarI GAAGNNNNNNTAC 2 cut(s) 4098, 4130
BauI CACGAG 2 cut(s) 5073, 7423
BbsI GAAGAC 4 cut(s) 2568, 4959, 6108, 7266
Bbv12I GWGCWC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
BceAI ACGGC 3 cut(s) 1062, 1318, 7671
BcgI CGANNNNNNTGC 2 cut(s) 5264, 5298
BciT130I CCWGG 9 cut(s) 68, 837, 1803, 2061, 2954, 5033, 6547, 7101, 7205
BclI TGATCA 3 cut(s) 2290, 4150, 6870
BcnI CCSGG 7 cut(s) 372, 2012, 3067, 3971, 4001, 6291, 6700
BfoI RGCGCY 2 cut(s) 4843, 7129
BfuAI ACCTGC 5 cut(s) 540, 1855, 2889, 4615, 5798
BglII AGATCT 3 cut(s) 2904, 4186, 6160
BlpI GCTNAGC 2 cut(s) 159, 6477
Bme18I GGWCC 7 cut(s) 63, 830, 2075, 2518, 5527, 6379, 6986
BmeRI GACNNNNNGTC 2 cut(s) 3483, 6838
BmeT110I CYCGRG 3 cut(s) 204, 6878, 7601
BmrI ACTGGG 4 cut(s) 771, 1417, 3336, 5158
BmuI ACTGGG 4 cut(s) 771, 1417, 3336, 5158
BoxI GACNNNNGTC 1 cut(s) 2714
BpiI GAAGAC 4 cut(s) 2568, 4959, 6108, 7266
BpmI CTGGAG 5 cut(s) 89, 957, 4521, 7093, 7575
Bpu10I CCTNAGC 4 cut(s) 3489, 3934, 4836, 5535
Bpu1102I GCTNAGC 2 cut(s) 159, 6477
BpuEI CTTGAG 7 cut(s) 1175, 2235, 3161, 4112, 4742, 7137, 7547
BpuMI CCSGG 7 cut(s) 372, 2012, 3067, 3971, 4001, 6291, 6700
BsaBI GATNNNNATC 2 cut(s) 6108, 6456
BsaI GGTCTC 2 cut(s) 2764, 6448
BsaWI WCCGGW 1 cut(s) 4553
BsaXI ACNNNNNCTCC 2 cut(s) 6150, 6180
Bse118I RCCGGY 1 cut(s) 4430
Bse3DI GCAATG 7 cut(s) 1126, 1728, 1870, 2073, 2868, 5043, 6873
Bse8I GATNNNNATC 2 cut(s) 6108, 6456
BseAI TCCGGA 1 cut(s) 4553
BseBI CCWGG 9 cut(s) 68, 837, 1803, 2061, 2954, 5033, 6547, 7101, 7205
BseJI GATNNNNATC 2 cut(s) 6108, 6456
BseMI GCAATG 7 cut(s) 1126, 1728, 1870, 2073, 2868, 5043, 6873
BseRI GAGGAG 4 cut(s) 2126, 5075, 5930, 7525
BseYI CCCAGC 2 cut(s) 5760, 7450
BsgI GTGCAG 4 cut(s) 1694, 1743, 4638, 6607
Bsh1236I CGCG 1 cut(s) 7293
Bsh1285I CGRYCG 1 cut(s) 6699
BshNI GGYRCC 1 cut(s) 3375
BsiEI CGRYCG 1 cut(s) 6699
BsiHKAI GWGCWC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
BsiHKCI CYCGRG 3 cut(s) 204, 6878, 7601
BslFI GGGAC 7 cut(s) 49, 739, 2924, 3327, 3724, 5687, 6365
BsmFI GGGAC 7 cut(s) 49, 739, 2924, 3327, 3724, 5687, 6365
BsmI GAATGC 5 cut(s) 217, 793, 1253, 1962, 6172
Bso31I GGTCTC 2 cut(s) 2764, 6448
BsoBI CYCGRG 3 cut(s) 204, 6878, 7601
Bsp1286I GDGCHC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
Bsp13I TCCGGA 1 cut(s) 4553
Bsp1407I TGTACA 1 cut(s) 6307
Bsp1720I GCTNAGC 2 cut(s) 159, 6477
Bsp19I CCATGG 3 cut(s) 6947, 7230, 7481
Bsp68I TCGCGA 1 cut(s) 7293
BspEI TCCGGA 1 cut(s) 4553
BspFNI CGCG 1 cut(s) 7293
BspHI TCATGA 5 cut(s) 1200, 3643, 4575, 5092, 5628
BspMAI CTGCAG 4 cut(s) 1797, 2886, 3442, 5413
BspMI ACCTGC 5 cut(s) 540, 1855, 2889, 4615, 5798
BspPI GGATC 7 cut(s) 95, 2002, 2373, 3084, 3252, 6869, 7432
BspT107I GGYRCC 1 cut(s) 3375
BspTNI GGTCTC 2 cut(s) 2764, 6448
BsrBI CCGCTC 1 cut(s) 5369
BsrDI GCAATG 7 cut(s) 1126, 1728, 1870, 2073, 2868, 5043, 6873
BsrFI RCCGGY 1 cut(s) 4430
BsrGI TGTACA 1 cut(s) 6307
BssAI RCCGGY 1 cut(s) 4430
BssSI CACGAG 2 cut(s) 5073, 7423
BssT1I CCWWGG 9 cut(s) 1109, 2514, 2577, 5424, 5797, 6650, 6947, 7230, 7481
Bst2BI CACGAG 2 cut(s) 5073, 7423
Bst2UI CCWGG 9 cut(s) 68, 837, 1803, 2061, 2954, 5033, 6547, 7101, 7205
Bst6I CTCTTC 7 cut(s) 954, 1249, 2101, 2343, 3264, 4233, 5211
BstAPI GCANNNNNTGC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 6307
BstDSI CCRYGG 3 cut(s) 6947, 7230, 7481
BstENI CCTNNNNNAGG 1 cut(s) 5801
BstFNI CGCG 1 cut(s) 7293
BstH2I RGCGCY 2 cut(s) 4843, 7129
BstHHI GCGC 5 cut(s) 3465, 4842, 5594, 5984, 7128
BstMCI CGRYCG 1 cut(s) 6699
BstMWI GCNNNNNNNGC 9 cut(s) 251, 773, 1325, 1886, 3947, 5008, 5279, 5756, 6487
BstNI CCWGG 9 cut(s) 68, 837, 1803, 2061, 2954, 5033, 6547, 7101, 7205
BstNSI RCATGY 9 cut(s) 622, 1817, 3518, 3543, 3954, 4225, 4688, 5519, 7710
BstPAI GACNNNNGTC 1 cut(s) 2714
BstUI CGCG 1 cut(s) 7293
BstV2I GAAGAC 4 cut(s) 2568, 4959, 6108, 7266
BstX2I RGATCY 6 cut(s) 87, 2904, 3076, 3244, 4186, 6160
BstXI CCANNNNNNTGG 2 cut(s) 3042, 5064
BstYI RGATCY 6 cut(s) 87, 2904, 3076, 3244, 4186, 6160
BtgI CCRYGG 3 cut(s) 6947, 7230, 7481
BtsI GCAGTG 3 cut(s) 774, 1686, 5828
BtuMI TCGCGA 1 cut(s) 7293
BveI ACCTGC 5 cut(s) 540, 1855, 2889, 4615, 5798
CaiI CAGNNNCTG 4 cut(s) 3326, 3446, 3758, 6682
CciI TCATGA 5 cut(s) 1200, 3643, 4575, 5092, 5628
CfoI GCGC 5 cut(s) 3465, 4842, 5594, 5984, 7128
Cfr10I RCCGGY 1 cut(s) 4430
CseI GACGC 1 cut(s) 1308
CsiI ACCWGGT 1 cut(s) 7099
CspCI CAANNNNNGTGG 4 cut(s) 5025, 5060, 7359, 7394
DraI TTTAAA 3 cut(s) 1941, 4216, 7305
DraIII CACNNNGTG 2 cut(s) 1717, 5121
DriI GACNNNNNGTC 2 cut(s) 3483, 6838
EaeI YGGCCR 1 cut(s) 5112
Eam1104I CTCTTC 7 cut(s) 954, 1249, 2101, 2343, 3264, 4233, 5211
Eam1105I GACNNNNNGTC 2 cut(s) 3483, 6838
EarI CTCTTC 7 cut(s) 954, 1249, 2101, 2343, 3264, 4233, 5211
EciI GGCGGA 2 cut(s) 445, 3561
Ecl136II GAGCTC 3 cut(s) 6593, 6907, 7559
Eco130I CCWWGG 9 cut(s) 1109, 2514, 2577, 5424, 5797, 6650, 6947, 7230, 7481
Eco147I AGGCCT 4 cut(s) 2513, 4383, 5825, 6956
Eco24I GRGCYC 3 cut(s) 6595, 6909, 7561
Eco31I GGTCTC 2 cut(s) 2764, 6448
Eco32I GATATC 2 cut(s) 2932, 3628
Eco47I GGWCC 7 cut(s) 63, 830, 2075, 2518, 5527, 6379, 6986
Eco47III AGCGCT 1 cut(s) 4841
Eco53kI GAGCTC 3 cut(s) 6593, 6907, 7559
Eco88I CYCGRG 3 cut(s) 204, 6878, 7601
EcoICRI GAGCTC 3 cut(s) 6593, 6907, 7559
EcoNI CCTNNNNNAGG 1 cut(s) 5801
EcoO109I RGGNCCY 3 cut(s) 830, 4717, 6379
EcoRI GAATTC 5 cut(s) 2402, 3145, 4085, 6562, 6739
EcoRII CCWGG 9 cut(s) 66, 835, 1801, 2059, 2952, 5031, 6545, 7099, 7203
EcoRV GATATC 2 cut(s) 2932, 3628
EcoT14I CCWWGG 9 cut(s) 1109, 2514, 2577, 5424, 5797, 6650, 6947, 7230, 7481
EcoT22I ATGCAT 2 cut(s) 1962, 3952
EcoT38I GRGCYC 3 cut(s) 6595, 6909, 7561
ErhI CCWWGG 9 cut(s) 1109, 2514, 2577, 5424, 5797, 6650, 6947, 7230, 7481
FalI AAGNNNNNCTT 2 cut(s) 7251, 7283
FaqI GGGAC 7 cut(s) 49, 739, 2924, 3327, 3724, 5687, 6365
FauI CCCGC 2 cut(s) 5383, 5664
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 3 cut(s) 2290, 4150, 6870
FblI GTMKAC 4 cut(s) 1322, 1392, 4123, 7647
FriOI GRGCYC 3 cut(s) 6595, 6909, 7561
FspI TGCGCA 1 cut(s) 5593
GlaI GCGC 5 cut(s) 3464, 4841, 5593, 5983, 7127
GsaI CCCAGC 2 cut(s) 5764, 7454
GsuI CTGGAG 5 cut(s) 89, 957, 4521, 7093, 7575
HaeII RGCGCY 2 cut(s) 4843, 7129
HgaI GACGC 1 cut(s) 1308
HhaI GCGC 5 cut(s) 3465, 4842, 5594, 5984, 7128
Hin6I GCGC 5 cut(s) 3463, 4840, 5592, 5982, 7126
HinP1I GCGC 5 cut(s) 3463, 4840, 5592, 5982, 7126
HincII GTYRAC 6 cut(s) 1610, 1782, 3048, 4407, 7648, 7712
HindII GTYRAC 6 cut(s) 1610, 1782, 3048, 4407, 7648, 7712
HindIII AAGCTT 6 cut(s) 1894, 2753, 3881, 5646, 6530, 6929
Hpy99I CGWCG 1 cut(s) 7649
HpyCH4IV ACGT 4 cut(s) 2889, 3050, 5964, 7225
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 773, 1325, 1886, 3947, 5008, 5279, 5756, 6487
HpySE526I ACGT 4 cut(s) 2889, 3050, 5964, 7225
HspAI GCGC 5 cut(s) 3463, 4840, 5592, 5982, 7126
KflI GGGWCCC 1 cut(s) 6379
Kpn2I TCCGGA 1 cut(s) 4553
Ksp22I TGATCA 3 cut(s) 2290, 4150, 6870
MabI ACCWGGT 1 cut(s) 7099
MaeII ACGT 4 cut(s) 2889, 3050, 5964, 7225
MbiI CCGCTC 1 cut(s) 5369
MflI RGATCY 6 cut(s) 87, 2904, 3076, 3244, 4186, 6160
MhlI GDGCHC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
MlsI TGGCCA 1 cut(s) 5114
MluNI TGGCCA 1 cut(s) 5114
MmeI TCCRAC 8 cut(s) 941, 1100, 1461, 2117, 2406, 2747, 7110, 7727
Mox20I TGGCCA 1 cut(s) 5114
Mph1103I ATGCAT 2 cut(s) 1962, 3952
MroI TCCGGA 1 cut(s) 4553
MscI TGGCCA 1 cut(s) 5114
MslI CAYNNNNRTG 3 cut(s) 798, 1352, 5103
Msp20I TGGCCA 1 cut(s) 5114
MssI GTTTAAAC 1 cut(s) 4216
Mva1269I GAATGC 5 cut(s) 217, 793, 1253, 1962, 6172
MvaI CCWGG 9 cut(s) 68, 837, 1803, 2061, 2954, 5033, 6547, 7101, 7205
MvnI CGCG 1 cut(s) 7293
MwoI GCNNNNNNNGC 9 cut(s) 251, 773, 1325, 1886, 3947, 5008, 5279, 5756, 6487
NciI CCSGG 7 cut(s) 372, 2012, 3067, 3971, 4001, 6291, 6700
NcoI CCATGG 3 cut(s) 6947, 7230, 7481
NdeI CATATG 1 cut(s) 286
NmuCI GTSAC 8 cut(s) 3113, 3475, 3748, 3787, 5138, 5859, 7234, 7457
NruI TCGCGA 1 cut(s) 7293
NsbI TGCGCA 1 cut(s) 5593
NsiI ATGCAT 2 cut(s) 1962, 3952
NspI RCATGY 9 cut(s) 622, 1817, 3518, 3543, 3954, 4225, 4688, 5519, 7710
PaeI GCATGC 4 cut(s) 1817, 3543, 4688, 5519
PagI TCATGA 5 cut(s) 1200, 3643, 4575, 5092, 5628
PaqCI CACCTGC 1 cut(s) 4615
PceI AGGCCT 4 cut(s) 2513, 4383, 5825, 6956
PciI ACATGT 4 cut(s) 618, 3514, 4221, 7706
PctI GAATGC 5 cut(s) 217, 793, 1253, 1962, 6172
PflFI GACNNNGTC 1 cut(s) 2133
PflMI CCANNNNNTGG 4 cut(s) 1115, 1408, 2766, 7255
PfoI TCCNGGA 2 cut(s) 2010, 2059
PmeI GTTTAAAC 1 cut(s) 4216
PpuMI RGGWCCY 2 cut(s) 830, 6379
PscI ACATGT 4 cut(s) 618, 3514, 4221, 7706
PshAI GACNNNNGTC 1 cut(s) 2714
PshBI ATTAAT 2 cut(s) 2991, 5499
PsiI TTATAA 2 cut(s) 2090, 2363
Psp124BI GAGCTC 3 cut(s) 6595, 6909, 7561
Psp1406I AACGTT 1 cut(s) 5964
Psp5II RGGWCCY 2 cut(s) 830, 6379
Psp6I CCWGG 9 cut(s) 66, 835, 1801, 2059, 2952, 5031, 6545, 7099, 7203
PspFI CCCAGC 2 cut(s) 5760, 7450
PspGI CCWGG 9 cut(s) 66, 835, 1801, 2059, 2952, 5031, 6545, 7099, 7203
PspPPI RGGWCCY 2 cut(s) 830, 6379
PsrI GAACNNNNNNTAC 4 cut(s) 2870, 2902, 4800, 4832
PstI CTGCAG 4 cut(s) 1797, 2886, 3442, 5413
PstNI CAGNNNCTG 4 cut(s) 3326, 3446, 3758, 6682
PsuI RGATCY 6 cut(s) 87, 2904, 3076, 3244, 4186, 6160
PsyI GACNNNGTC 1 cut(s) 2133
RruI TCGCGA 1 cut(s) 7293
RseI CAYNNNNRTG 3 cut(s) 798, 1352, 5103
SacI GAGCTC 3 cut(s) 6595, 6909, 7561
SalI GTCGAC 1 cut(s) 7646
SduI GDGCHC 7 cut(s) 247, 916, 4654, 4883, 6595, 6909, 7561
SexAI ACCWGGT 1 cut(s) 7099
SinI GGWCC 7 cut(s) 63, 830, 2075, 2518, 5527, 6379, 6986
SmiMI CAYNNNNRTG 3 cut(s) 798, 1352, 5103
SmlI CTYRAG 7 cut(s) 1154, 2250, 3140, 4127, 4721, 7152, 7562
SmoI CTYRAG 7 cut(s) 1154, 2250, 3140, 4127, 4721, 7152, 7562
SphI GCATGC 4 cut(s) 1817, 3543, 4688, 5519
SseBI AGGCCT 4 cut(s) 2513, 4383, 5825, 6956
SspI AATATT 5 cut(s) 1627, 1680, 5253, 5664, 7579
SstI GAGCTC 3 cut(s) 6595, 6909, 7561
StuI AGGCCT 4 cut(s) 2513, 4383, 5825, 6956
StyI CCWWGG 9 cut(s) 1109, 2514, 2577, 5424, 5797, 6650, 6947, 7230, 7481
TaiI ACGT 4 cut(s) 2892, 3053, 5967, 7228
TatI WGTACW 3 cut(s) 1361, 5543, 6307
TauI GCSGC 3 cut(s) 3546, 5372, 5750
TseFI GTSAC 8 cut(s) 3113, 3475, 3748, 3787, 5138, 5859, 7234, 7457
Tsp45I GTSAC 8 cut(s) 3113, 3475, 3748, 3787, 5138, 5859, 7234, 7457
TspGWI ACGGA 1 cut(s) 4409
Tth111I GACNNNGTC 1 cut(s) 2133
Van91I CCANNNNNTGG 4 cut(s) 1115, 1408, 2766, 7255
VpaK11BI GGWCC 7 cut(s) 63, 830, 2075, 2518, 5527, 6379, 6986
VspI ATTAAT 2 cut(s) 2991, 5499
XagI CCTNNNNNAGG 1 cut(s) 5801
XbaI TCTAGA 3 cut(s) 2923, 3025, 6191
XceI RCATGY 9 cut(s) 622, 1817, 3518, 3543, 3954, 4225, 4688, 5519, 7710
XcmI CCANNNNNNNNNTGG 1 cut(s) 1116
XmiI GTMKAC 4 cut(s) 1322, 1392, 4123, 7647
Zsp2I ATGCAT 2 cut(s) 1962, 3952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.