Rh4AG428300

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000223
Physical Location & Seq
Forward (+)
72408366 .. 72415104
6739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG428300.1

Sequence Viewer

Length: 5184 bp
ATGTCAGTCCGTTTGAAGAGGTCAGTGCTAAATGTTCTACTAAAGCTTGCCAGTGGCTGGAGACCGAAGATGCAAAATTCAGATGTAATCTGTGGCAACTCTTCACTGATCTTGAAGAAATTCAAGGTTGAAGATCTATATATTGTTTGCACAACAGACATAGCAAAGGATTTGAGATACATTCAAGTATTAAAGAATTGGGACATATTGCCTATAGAGGATGTCCCAACGTTGGTAAATCGCCTGGACAATATATTAAACAAATATACAGATGATTATATTAATCTCTGCAAGGAGAAATGTCTTGAGGGTGATTTGGAAGTTCCAAAGAGCTGGCCAAGTTCTTTGGAATTTATTCGGTTTAAGGATCTTAGCTTCACTGAAATCCAGAATGATTTAGTTGGTGACACTTCTCATGGTAGAAGTTATGCTGAGAATTCACGTGTCAGCGAAAGTTTGTTACTGATGAAATTTTACTCACTATCATCTGGGGTGGTAAACCACTTGCTGTCTGATCATGAGGGTAGAGAACTGGATCTTCCGTTTGAAGTTACAGACCAAGAATTGGAGATTATCCTTTATCAGAGAAGTTCCTTTATAGTTGGACGGTCAGGAACTGGGAAAACCACAGTTTTAACCATGAAATTATTTCAGAAAGAGCAGTGGCACCAGTTGGCAGTGCGAGGATGCCATATTAGTGAAAGCAACAAGGTTGAACAGAGTTCGGCAGCCACTGAGGGGAAAGTTCTACACCAGCTTTTCGTGACAGTGAGTCCGAAGCTATGTTATGCTATCAAGCAACATGTTTTACACTTGAAAAGATTTGCATCTGGTAGAAGTCATTCAACTGAAACCGGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTTAACGATATCCAAGATTCATTTCATGAAGTTCCTGCCCATTCTTACCCTCTTTTCATCACATTGCATAAGTTCTTGATTATGCTTGATGGAACATTGAGAAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGGTTACTCATGGTCAACTACGAAGTTCAAGATCAATTGCCTTGCAGACTTTTATAAGGACAAAGGAAGTCAGTTATGAGAAGTTTAGTTCATTATACTGGCCTCATTTTAATACCGAGTTAACAAAGAAGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGCCTGGGAGCTCTAGAAGCGTGTGATGGTAAATTGAGTCGAGAGGATTATGTTCAACTGTCAGAGAGCCGAGCTTCCAATCTAACCAGCCAAGAGCGAGAAGTTATATATGACATTTTTCAGATGTATGAAAAAATGAAGTTGGAATATGGTGAATTTGATCTTGCTGATTTTGTTACTGATCTTCACCGTCGACTCAGGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATATTGATGAGGTTCAGGATCTCACAATGAGTCAAATTGCCTTGTTTAAACACATGTGCCACAATGTTGAAGAGGGTTTTGTTTTTTCGGGTGATACTGCTCAAACAATTGCAAGGGGTATTGATTTCAGATTTCAAGAATTACGACATCTATTCTACAAGAAATTTATGTTGGAGTCAAGAAGCAATAAGCACTATGAAGGTAAGGAAAAGGGCCAAATCTCAGAAATTTTTCACTTGACTCAAAACTTTCGTACCCATGCTGGAATCCTCAAGCTATCACAAAGCATTGTTGAACTTCTTTATCATTTTTTCCCCCTTTCCATTGATGTTTTAAAACCTGAAAGCAGTTTGATATATGGGGAAGCTCCAGTATTGCTTGAATCTGGAGAAAATGAAAATGCAATCATAAAGATATTTGGAAATACTGGAAATATAGTTGGATTTGGTGCTGAGCAGGTAATTTTGGTGCGAGATGATAGTGCTCGAAATGAGATATCAAAACTTGTAGGGAAGCATGCTCTTGTGCTAACCATTGTCGAGTGCAAGGGCCTTGAGTTTCAGGATGTTCTCTTATACAACTTTTTCGGATCATCACCTTCGAGAAATCAATGGAGGGTAATATACGATTACATGAATGAACTAGATTTACTCGACCACACATTACCCAGGGGCTTTCCAAGTTTTAATGTAGCCAAACACAACATATTGTGCTCTGAACTGAAACAACTATATGTGGCTGTTACACGCACGAGACAGAGATTGTGGATATGTGAGAACTTTGAAGATCTCTCCAAACCAATGTTTGACTACTGGAAGAAGAAGTGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGCAATGCAAGTTGCAAGCAGTCCAGAGGAGTGGAAGTCACGTGGCATGAAGCTATATCATGAACATAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCAGGTGATGCTTACTGGGAAAGAAGGTCTAAAGCTGCTGGTCTTAAAGCACTCGCTGACCATATGCGAACGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTGATGCTATAGGCAAGGCAGATTCTGCGGCGAGATGTTTTTCTGATTTGGGGGAGTATGAAAGAGCTGCTAGGATATACTTGAACAAATGTGGCGAGTCTGAACTGGAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGATGCTATGAGGAGGCTGCAGATGTGTATGCCAAGGGAAATTTTTTCTCAGAGTGTCTCACTGTTTGTGGCAAGGGAAGACTATTTGAAATGGGTTTGCAATACATCAATAATTGGAAACAGCATGCAGAAGAGGACAGTGCTATGAGTACAAGGGGAAATGGAATAAATAGAATGGAGCATGAGTTTTTAGAGAGCTGTGCACTTCACTATTATAATTTGAATGATAACAGATCCATGATGAAATTTGTTAAAGCTTTTCATTCTATAATTTTGATGCGAAACTTTTTGAAGAGGTTGGATAACCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCAAACATCGCACAGCTTAAAGGTGATATTCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAGGCCTCACTGCACATACTATTCTACGTACTTGCCCAGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCTGAAGAAGTTTCCACGACAGGAGGCTCTTTTGGCACAAGCCAAGTCACTTGCCAAGAATGAGACAGACAACTTTTATGAATTGGTTTCCACTGAGGTTGATATTTTATTAAATGGGATGGATAGGTTGGCTGTTTTGAAAAATCAAATCAATTCTTCTGAGAGACATAAGAGTATTAGAGGGGAAGTGTTATCAGCTAGAAAAATTTTGGATGCCCATCTTTCTCGAAGAACTGATAAGTATATATGGGAAACAGAATTGATTGGTGATCTTGTACAGCATTCAGAAGAGAAAATATCTAAAAATCAAGTTTCCATCGATTCACTCATATACTTCTGGAATTCTTGGAAGGAGAAGATTCTTCACATAATTGAATCTGTAGGTTGTTTGGAAACACAAGACTACAAAGGTTATGTAGAGTTTGGTTTTACTTTCTTAGGAGTGTGGAGGCTGTATCATAATCTGAGTCCAGTGTATGTTTTACTCATCTCTGATGCTGATTGGGTTAGAGGTCTGGACAAAAGACATTTCCAAAGTTATGAGAAGTTGGTCTCCATTGATGTTCACCAACTCATTCCAGCTGCTCAAAATTTTTGGTGTTCAGAACTGGTCTCTGTTGGCATTAAGGTTTTGGAAAAGCTTGAAGCACTTTTCAAGTTCCCAGTCAAGAGTCCTGATTCAATTTTCTTTCAAAGCAGGGCTCTTACTCTGATCTATGATGTTGGAAAATATCTTTTGGATTCCAAGTGTCTGAAGCAAAGGAATCAAGATACCATGACACTACAGAAGTTTGTAACCTTTTCGACTGAAAAATATATTACTTGCATATTTCCTTTAGATTGGAGGAATTCAGCAAGAGAGAATATGATTTCTCTTAGAAGAACTGATGCTTCCCAATCTATGCTGAAACAAGTAATAGTTGAATGTATCAGCGGGAAGAACAAGATGTCTTATGGGCAAATTGGAAACGTCATCATGATCATTCTTGGGTCTGGTAAGCCTAGTAGTGAACTTCACAGCATGATTGTGCAAAAGCTGACCTGCAACCCGCCATGGATGTCATTCGTTGAGACTTTGTGTGAGAAGGATATGGGACCTTGGAGTCGTAGTGGAGAACCTAAACAGACATCCGTTTTGTGGCGATTCTATGAAGCATTAGTCGAAACTTATAGTGCAAACTGGAGGGCTCTTCATGACTACATATCACCTGGTTGCTTCTTGTATCTTGTAGAGCGCCTTTTGACTTTGGCCTCTTGCTTTCATGGTTTTGTTATCTCCACGAGTTCAAGTTTTATAGAATGGCTGATATACAAGGATGGAGATACCATCATAAGTTCACCATCCCTGGGAGAGATTGCTGATTTTGTGACTGATGTTGTTCGAGATTGTATTTATAAAAGGGGGAATATGATTGATTGGATTAGAAAAACTGATGCAGATGGGAAGAGTTCTTATCCACTACTCATGTTGAGATTGGTTATTCTACTATGCTTGCTTCTTGTGAACTTCGGGAAGTCTTTAGATATGCTCTTTGATATTCTGGGGAAGAACTATGTCATTGGAGTACTGCCCAAGGAATTTGTTGAGGCCCTTAAGAAGGGGAGGAAAGACAGATCTGCGAGTGCCTGTGTGCTAGCTATAGCTACTGCTCTTAAGAAGATTGGTAATCCTTTAGTAATTGCAAGTTTCGGGTTCAATTGTTCAAGGTTCTTATGCACAGATGCCATTTATGTTGACATGGTATTAAACCCGAGCAAGGATGACGTGCTTGGGAAATTCTTCCCACCTGTTGTCCCAGCTTCACAAAGCCGAATGAGCCCTGTTGAAGGGGAAGGTAATAATCGTTATACTGTTCTGTCGGATGCTGAAGACACTCACAGTGAAGATGAGGAGATTTGCATTTCCAATGAGTCGAAGCCGAAGGGCCCAGAACCTGATGCTGCTTCTGCAACCCATGGGAAGAAAAAGGGCAATCCCAAGAAACACAAGGGGAAAAAGGGCCGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1727

Amino Acids

197.52

Weight (kDa)

6.22

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 425 - 520 3e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1088, 2889, 4569
AarI CACCTGC 1 cut(s) 2408
AasI GACNNNNNNGTC 1 cut(s) 4275
Acc36I ACCTGC 4 cut(s) 1897, 2408, 3080, 4223
AccB1I GGYRCC 1 cut(s) 666
AccB7I CCANNNNNTGG 2 cut(s) 57, 565
AccI GTMKAC 1 cut(s) 1405
AccII CGCG 1 cut(s) 2654
AciI CCGC 5 cut(s) 2564, 2652, 2654, 4107, 4223
AclI AACGTT 1 cut(s) 230
AclWI GGATC 6 cut(s) 375, 543, 1440, 1476, 2047, 2901
AcoI YGGCCR 2 cut(s) 335, 2394
AcuI CTGAAG 4 cut(s) 2517, 3197, 3947, 5061
AcvI CACGTG 2 cut(s) 443, 2357
AdeI CACNNNGTG 1 cut(s) 2403
AfaI GTAC 5 cut(s) 1705, 2824, 3132, 3450, 4740
AflII CTTAAG 2 cut(s) 4766, 4826
AflIII ACRYGT 3 cut(s) 442, 802, 1503
AgeI ACCGGT 1 cut(s) 854
AhdI GACNNNNNGTC 1 cut(s) 771
AjiI CACGTC 1 cut(s) 4939
AjnI CCWGG 5 cut(s) 243, 1215, 2117, 4381, 4518
AjuI GAANNNNNNNTTGG 4 cut(s) 903, 935, 1604, 1636
AloI GAACNNNNNNTCC 2 cut(s) 2476, 2508
Alw21I GWGCWC 4 cut(s) 1225, 1936, 2165, 2878
Alw26I GTCTC 9 cut(s) 55, 2197, 2735, 3147, 3230, 3331, 3730, 3790, 4238
Alw44I GTGCAC 1 cut(s) 2874
AlwI GGATC 6 cut(s) 375, 543, 1440, 1476, 2047, 2901
AlwNI CAGNNNCTG 5 cut(s) 57, 617, 734, 2561, 5108
Ama87I CYCGRG 1 cut(s) 4924
ApaI GGGCCC 1 cut(s) 5102
ApaLI GTGCAC 1 cut(s) 2874
ApeKI GCWGC 9 cut(s) 728, 2450, 2525, 2603, 2690, 3029, 3159, 3755, 5114
ArsI GACNNNNNNTTYG 2 cut(s) 4291, 4323
AseI ATTAAT 1 cut(s) 282
AsiGI ACCGGT 1 cut(s) 854
Asp700I GAANNNNTTC 5 cut(s) 119, 354, 841, 1680, 4952
AspLEI GCGC 1 cut(s) 4410
AspS9I GGNCC 7 cut(s) 1663, 1999, 4268, 4762, 5098, 5099, 5173
AsuNHI GCTAGC 1 cut(s) 4807
AvaI CYCGRG 1 cut(s) 4924
AvaII GGWCC 1 cut(s) 4268
BaeGI GKGCMC 2 cut(s) 2878, 5102
BaeI ACNNNNGTAYC 2 cut(s) 1687, 1720
BalI TGGCCA 2 cut(s) 337, 2396
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 5 cut(s) 1225, 3877, 4363, 4994, 5102
BauI CACGAG 2 cut(s) 2200, 4453
BbrPI CACGTG 2 cut(s) 443, 2357
BbsI GAAGAC 2 cut(s) 2758, 5049
Bbv12I GWGCWC 4 cut(s) 1225, 1936, 2165, 2878
BbvI GCAGC 9 cut(s) 740, 2437, 2512, 2590, 2677, 3016, 3171, 3742, 5101
BccI CCATC 9 cut(s) 980, 1232, 3286, 3399, 3497, 4484, 4508, 4522, 4607
BciT130I CCWGG 5 cut(s) 245, 1217, 2119, 4383, 4520
BclI TGATCA 2 cut(s) 514, 4152
BcoDI GTCTC 9 cut(s) 55, 2197, 2735, 3147, 3230, 3331, 3730, 3790, 4238
BfaI CTAG 7 cut(s) 1226, 2093, 2607, 2669, 3372, 4176, 4808
BfmI CTRYAG 6 cut(s) 213, 2544, 2691, 3552, 3956, 4812
BfoI RGCGCY 1 cut(s) 4411
BfrI CTTAAG 2 cut(s) 4766, 4826
BfuAI ACCTGC 4 cut(s) 1897, 2408, 3080, 4223
BglII AGATCT 3 cut(s) 133, 2236, 4787
BlpI GCTNAGC 1 cut(s) 1902
BmcAI AGTACT 1 cut(s) 4740
Bme1390I CCNGG 5 cut(s) 245, 1217, 2119, 4383, 4520
Bme18I GGWCC 1 cut(s) 4268
BmeRI GACNNNNNGTC 1 cut(s) 771
BmeT110I CYCGRG 1 cut(s) 4924
BmgBI CACGTC 1 cut(s) 4939
BmgT120I GGNCC 7 cut(s) 1663, 1999, 4268, 4762, 5098, 5099, 5173
BmiI GGNNCC 3 cut(s) 668, 4269, 5100
BmrFI CCNGG 5 cut(s) 245, 1217, 2119, 4383, 4520
BmrI ACTGGG 4 cut(s) 627, 2440, 3133, 3830
BmtI GCTAGC 1 cut(s) 4811
BmuI ACTGGG 4 cut(s) 627, 2440, 3133, 3830
BpiI GAAGAC 2 cut(s) 2758, 5049
BpmI CTGGAG 5 cut(s) 79, 1803, 1857, 2663, 4375
Bpu1102I GCTNAGC 1 cut(s) 1902
BpuEI CTTGAG 3 cut(s) 326, 1706, 2024
Bsa29I ATCGAT 1 cut(s) 3492
BsaAI YACGTR 3 cut(s) 443, 2357, 3130
BsaBI GATNNNNATC 2 cut(s) 826, 3390
BsaI GGTCTC 3 cut(s) 55, 3730, 3790
BsaWI WCCGGW 1 cut(s) 854
BsaXI ACNNNNNCTCC 6 cut(s) 2336, 2366, 2843, 2873, 4267, 4297
Bse118I RCCGGY 1 cut(s) 854
Bse3DI GCAATG 2 cut(s) 959, 2325
Bse8I GATNNNNATC 2 cut(s) 826, 3390
BseBI CCWGG 5 cut(s) 245, 1217, 2119, 4383, 4520
BseCI ATCGAT 1 cut(s) 3492
BseJI GATNNNNATC 2 cut(s) 826, 3390
BseMI GCAATG 2 cut(s) 959, 2325
BseRI GAGGAG 3 cut(s) 2357, 2699, 5078
BseSI GKGCMC 2 cut(s) 2878, 5102
BseXI GCAGC 9 cut(s) 740, 2437, 2512, 2590, 2677, 3016, 3171, 3742, 5101
BseYI CCCAGC 1 cut(s) 4969
BsgI GTGCAG 1 cut(s) 3098
Bsh1236I CGCG 1 cut(s) 2654
BshNI GGYRCC 1 cut(s) 666
BshTI ACCGGT 1 cut(s) 854
BshVI ATCGAT 1 cut(s) 3492
BsiHKAI GWGCWC 4 cut(s) 1225, 1936, 2165, 2878
BsiHKCI CYCGRG 1 cut(s) 4924
BsiSI CCGG 1 cut(s) 855
BslFI GGGAC 4 cut(s) 209, 215, 4281, 4952
BsmAI GTCTC 9 cut(s) 55, 2197, 2735, 3147, 3230, 3331, 3730, 3790, 4238
BsmFI GGGAC 4 cut(s) 209, 215, 4281, 4952
BsmI GAATGC 1 cut(s) 3454
Bso31I GGTCTC 3 cut(s) 55, 3730, 3790
BsoBI CYCGRG 1 cut(s) 4924
Bsp120I GGGCCC 1 cut(s) 5098
Bsp1286I GDGCHC 8 cut(s) 1225, 1936, 2165, 2878, 3877, 4363, 4994, 5102
Bsp1407I TGTACA 1 cut(s) 3448
Bsp1720I GCTNAGC 1 cut(s) 1902
Bsp19I CCATGG 2 cut(s) 4226, 5128
BspACI CCGC 5 cut(s) 2564, 2652, 2654, 4107, 4223
BspDI ATCGAT 1 cut(s) 3492
BspFNI CGCG 1 cut(s) 2654
BspHI TCATGA 5 cut(s) 517, 922, 2374, 4149, 4366
BspLI GGNNCC 3 cut(s) 668, 4269, 5100
BspMAI CTGCAG 1 cut(s) 2695
BspMI ACCTGC 4 cut(s) 1897, 2408, 3080, 4223
BspOI GCTAGC 1 cut(s) 4811
BspPI GGATC 6 cut(s) 375, 543, 1440, 1476, 2047, 2901
BspQI GCTCTTC 1 cut(s) 4368
BspT107I GGYRCC 1 cut(s) 666
BspTI CTTAAG 2 cut(s) 4766, 4826
BspTNI GGTCTC 3 cut(s) 55, 3730, 3790
BsrDI GCAATG 2 cut(s) 959, 2325
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 3448
BssAI RCCGGY 1 cut(s) 854
BssSI CACGAG 2 cut(s) 2200, 4453
BssT1I CCWWGG 6 cut(s) 2706, 3079, 4226, 4271, 4746, 5128
Bst2BI CACGAG 2 cut(s) 2200, 4453
Bst2UI CCWGG 5 cut(s) 245, 1217, 2119, 4383, 4520
Bst4CI ACNGT 9 cut(s) 609, 631, 769, 1272, 1403, 2737, 2813, 5026, 5054
Bst6I CTCTTC 9 cut(s) 11, 106, 1515, 2493, 2799, 2960, 3456, 4368, 4613
BstAFI CTTAAG 2 cut(s) 4766, 4826
BstAPI GCANNNNNTGC 2 cut(s) 2423, 2561
BstAUI TGTACA 1 cut(s) 3448
BstBAI YACGTR 3 cut(s) 443, 2357, 3130
BstC8I GCNNGC 8 cut(s) 48, 335, 1968, 2332, 2799, 3170, 4667, 4809
BstDSI CCRYGG 3 cut(s) 2652, 4226, 5128
BstENI CCTNNNNNAGG 3 cut(s) 3083, 4769, 4998
BstFNI CGCG 1 cut(s) 2654
BstH2I RGCGCY 1 cut(s) 4411
BstHHI GCGC 1 cut(s) 4410
BstMAI GTCTC 9 cut(s) 55, 2197, 2735, 3147, 3230, 3331, 3730, 3790, 4238
BstMWI GCNNNNNNNGC 9 cut(s) 1229, 2290, 2423, 2561, 3038, 3165, 3206, 4989, 5120
BstNI CCWGG 5 cut(s) 245, 1217, 2119, 4383, 4520
BstNSI RCATGY 5 cut(s) 806, 1507, 1970, 2318, 2801
BstSCI CCNGG 5 cut(s) 243, 1215, 2117, 4381, 4518
BstSFI CTRYAG 6 cut(s) 213, 2544, 2691, 3552, 3956, 4812
BstSLI GKGCMC 2 cut(s) 2878, 5102
BstSNI TACGTA 1 cut(s) 3130
BstUI CGCG 1 cut(s) 2654
BstV1I GCAGC 9 cut(s) 740, 2437, 2512, 2590, 2677, 3016, 3171, 3742, 5101
BstV2I GAAGAC 2 cut(s) 2758, 5049
BstX2I RGATCY 7 cut(s) 133, 367, 535, 1468, 2236, 2906, 4787
BstXI CCANNNNNNTGG 2 cut(s) 333, 2346
BstYI RGATCY 7 cut(s) 133, 367, 535, 1468, 2236, 2906, 4787
Bsu15I ATCGAT 1 cut(s) 3492
BsuTUI ATCGAT 1 cut(s) 3492
BtgI CCRYGG 3 cut(s) 2652, 4226, 5128
BtgZI GCGATG 1 cut(s) 3022
BtrI CACGTC 1 cut(s) 4939
BtsI GCAGTG 3 cut(s) 668, 684, 3110
BveI ACCTGC 4 cut(s) 1897, 2408, 3080, 4223
Cac8I GCNNGC 8 cut(s) 48, 335, 1968, 2332, 2799, 3170, 4667, 4809
CaiI CAGNNNCTG 5 cut(s) 57, 617, 734, 2561, 5108
CciI TCATGA 5 cut(s) 517, 922, 2374, 4149, 4366
CfoI GCGC 1 cut(s) 4410
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 7 cut(s) 1663, 1999, 4268, 4762, 5098, 5099, 5173
Cfr42I CCGCGG 1 cut(s) 2655
ClaI ATCGAT 1 cut(s) 3492
CsiI ACCWGGT 1 cut(s) 4381
Csp6I GTAC 5 cut(s) 1704, 2823, 3131, 3449, 4739
CspAI ACCGGT 1 cut(s) 854
CspCI CAANNNNNGTGG 2 cut(s) 3253, 3288
CviQI GTAC 5 cut(s) 1704, 2823, 3131, 3449, 4739
DraI TTTAAA 2 cut(s) 1498, 1785
DraIII CACNNNGTG 1 cut(s) 2403
DrdI GACNNNNNNGTC 1 cut(s) 4275
DriI GACNNNNNGTC 1 cut(s) 771
DseDI GACNNNNNNGTC 1 cut(s) 4275
EaeI YGGCCR 2 cut(s) 335, 2394
Eam1104I CTCTTC 9 cut(s) 11, 106, 1515, 2493, 2799, 2960, 3456, 4368, 4613
Eam1105I GACNNNNNGTC 1 cut(s) 771
EarI CTCTTC 9 cut(s) 11, 106, 1515, 2493, 2799, 2960, 3456, 4368, 4613
Ecl136II GAGCTC 1 cut(s) 1223
Eco105I TACGTA 1 cut(s) 3130
Eco130I CCWWGG 6 cut(s) 2706, 3079, 4226, 4271, 4746, 5128
Eco147I AGGCCT 1 cut(s) 3107
Eco24I GRGCYC 5 cut(s) 1225, 3877, 4363, 4994, 5102
Eco31I GGTCTC 3 cut(s) 55, 3730, 3790
Eco32I GATATC 2 cut(s) 907, 1947
Eco47I GGWCC 1 cut(s) 4268
Eco53kI GAGCTC 1 cut(s) 1223
Eco57I CTGAAG 4 cut(s) 2517, 3197, 3947, 5061
Eco72I CACGTG 2 cut(s) 443, 2357
Eco88I CYCGRG 1 cut(s) 4924
EcoICRI GAGCTC 1 cut(s) 1223
EcoNI CCTNNNNNAGG 3 cut(s) 3083, 4769, 4998
EcoO109I RGGNCCY 4 cut(s) 1999, 4268, 4762, 5098
EcoRI GAATTC 3 cut(s) 436, 3514, 4021
EcoRII CCWGG 5 cut(s) 243, 1215, 2117, 4381, 4518
EcoRV GATATC 2 cut(s) 907, 1947
EcoT14I CCWWGG 6 cut(s) 2706, 3079, 4226, 4271, 4746, 5128
EcoT38I GRGCYC 5 cut(s) 1225, 3877, 4363, 4994, 5102
ErhI CCWWGG 6 cut(s) 2706, 3079, 4226, 4271, 4746, 5128
FaqI GGGAC 4 cut(s) 209, 215, 4281, 4952
FauI CCCGC 2 cut(s) 4100, 4230
FauNDI CATATG 2 cut(s) 1437, 2478
FbaI TGATCA 2 cut(s) 514, 4152
FblI GTMKAC 1 cut(s) 1405
FriOI GRGCYC 5 cut(s) 1225, 3877, 4363, 4994, 5102
FspBI CTAG 7 cut(s) 1226, 2093, 2607, 2669, 3372, 4176, 4808
GlaI GCGC 1 cut(s) 4409
GsaI CCCAGC 1 cut(s) 4973
GsuI CTGGAG 5 cut(s) 79, 1803, 1857, 2663, 4375
HaeII RGCGCY 1 cut(s) 4411
HapII CCGG 1 cut(s) 855
HhaI GCGC 1 cut(s) 4410
Hin6I GCGC 1 cut(s) 4408
HinP1I GCGC 1 cut(s) 4408
HincII GTYRAC 4 cut(s) 1049, 1155, 1406, 4909
HindII GTYRAC 4 cut(s) 1049, 1155, 1406, 4909
HindIII AAGCTT 4 cut(s) 44, 1163, 2928, 3812
HpaI GTTAAC 1 cut(s) 1155
HpaII CCGG 1 cut(s) 855
Hpy99I CGWCG 1 cut(s) 1407
HpyAV CCTTC 9 cut(s) 1643, 2058, 2433, 3517, 4252, 4765, 4994, 5000, 5089
HpyCH4III ACNGT 9 cut(s) 609, 631, 769, 1272, 1403, 2737, 2813, 5026, 5054
HpyCH4IV ACGT 7 cut(s) 230, 442, 2356, 2486, 3129, 4143, 4938
HpyF10VI GCNNNNNNNGC 9 cut(s) 1229, 2290, 2423, 2561, 3038, 3165, 3206, 4989, 5120
HpySE526I ACGT 7 cut(s) 230, 442, 2356, 2486, 3129, 4143, 4938
HspAI GCGC 1 cut(s) 4408
Ksp22I TGATCA 2 cut(s) 514, 4152
KspAI GTTAAC 1 cut(s) 1155
KspI CCGCGG 1 cut(s) 2655
LguI GCTCTTC 1 cut(s) 4368
LmnI GCTCC 3 cut(s) 1220, 1822, 2851
Lsp1109I GCAGC 9 cut(s) 740, 2437, 2512, 2590, 2677, 3016, 3171, 3742, 5101
MabI ACCWGGT 1 cut(s) 4381
MaeI CTAG 7 cut(s) 1226, 2093, 2607, 2669, 3372, 4176, 4808
MaeII ACGT 7 cut(s) 230, 442, 2356, 2486, 3129, 4143, 4938
MfeI CAATTG 3 cut(s) 1068, 1557, 4870
MflI RGATCY 7 cut(s) 133, 367, 535, 1468, 2236, 2906, 4787
MhlI GDGCHC 8 cut(s) 1225, 1936, 2165, 2878, 3877, 4363, 4994, 5102
MlsI TGGCCA 2 cut(s) 337, 2396
MluNI TGGCCA 2 cut(s) 337, 2396
MmeI TCCRAC 8 cut(s) 583, 1335, 1602, 1870, 2952, 2979, 3877, 5013
Mox20I TGGCCA 2 cut(s) 337, 2396
MroXI GAANNNNTTC 5 cut(s) 119, 354, 841, 1680, 4952
MscI TGGCCA 2 cut(s) 337, 2396
MslI CAYNNNNRTG 2 cut(s) 696, 4199
Msp20I TGGCCA 2 cut(s) 337, 2396
MspA1I CMGCKG 3 cut(s) 2654, 3755, 4107
MspCI CTTAAG 2 cut(s) 4766, 4826
MspI CCGG 1 cut(s) 855
MspR9I CCNGG 5 cut(s) 245, 1217, 2119, 4383, 4520
MssI GTTTAAAC 1 cut(s) 1498
MunI CAATTG 3 cut(s) 1068, 1557, 4870
Mva1269I GAATGC 1 cut(s) 3454
MvaI CCWGG 5 cut(s) 245, 1217, 2119, 4383, 4520
MvnI CGCG 1 cut(s) 2654
MwoI GCNNNNNNNGC 9 cut(s) 1229, 2290, 2423, 2561, 3038, 3165, 3206, 4989, 5120
NcoI CCATGG 2 cut(s) 4226, 5128
NdeI CATATG 2 cut(s) 1437, 2478
NheI GCTAGC 1 cut(s) 4807
NlaIV GGNNCC 3 cut(s) 668, 4269, 5100
NmeAIII GCCGAG 1 cut(s) 1307
NmuCI GTSAC 5 cut(s) 404, 763, 2352, 3219, 4540
NspI RCATGY 5 cut(s) 806, 1507, 1970, 2318, 2801
PaeI GCATGC 2 cut(s) 1970, 2801
PagI TCATGA 5 cut(s) 517, 922, 2374, 4149, 4366
PaqCI CACCTGC 1 cut(s) 2408
PasI CCCWGGG 2 cut(s) 2118, 4519
PceI AGGCCT 1 cut(s) 3107
PciI ACATGT 2 cut(s) 802, 1503
PciSI GCTCTTC 1 cut(s) 4368
PctI GAATGC 1 cut(s) 3454
PdmI GAANNNNTTC 5 cut(s) 119, 354, 841, 1680, 4952
PflMI CCANNNNNTGG 2 cut(s) 57, 565
PinAI ACCGGT 1 cut(s) 854
PmaCI CACGTG 2 cut(s) 443, 2357
PmeI GTTTAAAC 1 cut(s) 1498
PmlI CACGTG 2 cut(s) 443, 2357
Ppu21I YACGTR 3 cut(s) 443, 2357, 3130
PpuMI RGGWCCY 1 cut(s) 4268
PscI ACATGT 2 cut(s) 802, 1503
PshBI ATTAAT 1 cut(s) 282
PsiI TTATAA 3 cut(s) 1088, 2889, 4569
Psp124BI GAGCTC 1 cut(s) 1225
Psp1406I AACGTT 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 4268
Psp6I CCWGG 5 cut(s) 243, 1215, 2117, 4381, 4518
PspCI CACGTG 2 cut(s) 443, 2357
PspFI CCCAGC 1 cut(s) 4969
PspGI CCWGG 5 cut(s) 243, 1215, 2117, 4381, 4518
PspN4I GGNNCC 3 cut(s) 668, 4269, 5100
PspOMI GGGCCC 1 cut(s) 5098
PspPI GGNCC 7 cut(s) 1663, 1999, 4268, 4762, 5098, 5099, 5173
PspPPI RGGWCCY 1 cut(s) 4268
PsrI GAACNNNNNNTAC 2 cut(s) 2082, 2114
PstI CTGCAG 1 cut(s) 2695
PstNI CAGNNNCTG 5 cut(s) 57, 617, 734, 2561, 5108
PsuI RGATCY 7 cut(s) 133, 367, 535, 1468, 2236, 2906, 4787
PvuII CAGCTG 1 cut(s) 3755
RsaI GTAC 5 cut(s) 1705, 2824, 3132, 3450, 4740
RsaNI GTAC 5 cut(s) 1704, 2823, 3131, 3449, 4739
RseI CAYNNNNRTG 2 cut(s) 696, 4199
SacI GAGCTC 1 cut(s) 1225
SacII CCGCGG 1 cut(s) 2655
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 1 cut(s) 4368
Sau96I GGNCC 7 cut(s) 1663, 1999, 4268, 4762, 5098, 5099, 5173
ScaI AGTACT 1 cut(s) 4740
ScrFI CCNGG 5 cut(s) 245, 1217, 2119, 4383, 4520
SduI GDGCHC 8 cut(s) 1225, 1936, 2165, 2878, 3877, 4363, 4994, 5102
SexAI ACCWGGT 1 cut(s) 4381
SfcI CTRYAG 6 cut(s) 213, 2544, 2691, 3552, 3956, 4812
Sfr303I CCGCGG 1 cut(s) 2655
SgrBI CCGCGG 1 cut(s) 2655
SinI GGWCC 1 cut(s) 4268
SmiMI CAYNNNNRTG 2 cut(s) 696, 4199
SmlI CTYRAG 5 cut(s) 305, 1721, 2003, 4766, 4826
SmoI CTYRAG 5 cut(s) 305, 1721, 2003, 4766, 4826
SnaBI TACGTA 1 cut(s) 3130
SphI GCATGC 2 cut(s) 1970, 2801
SseBI AGGCCT 1 cut(s) 3107
SsiI CCGC 5 cut(s) 2564, 2652, 2654, 4107, 4223
SspI AATATT 1 cut(s) 2535
SspMI CTAG 7 cut(s) 1226, 2093, 2607, 2669, 3372, 4176, 4808
SstI GAGCTC 1 cut(s) 1225
StuI AGGCCT 1 cut(s) 3107
StyD4I CCNGG 5 cut(s) 243, 1215, 2117, 4381, 4518
StyI CCWWGG 6 cut(s) 2706, 3079, 4226, 4271, 4746, 5128
TaaI ACNGT 9 cut(s) 609, 631, 769, 1272, 1403, 2737, 2813, 5026, 5054
TaiI ACGT 7 cut(s) 233, 445, 2359, 2489, 3132, 4146, 4941
TaqII GACCGA 1 cut(s) 79
TatI WGTACW 3 cut(s) 2822, 3448, 4738
TauI GCSGC 2 cut(s) 2567, 2654
TseFI GTSAC 5 cut(s) 404, 763, 2352, 3219, 4540
TseI GCWGC 9 cut(s) 728, 2450, 2525, 2603, 2690, 3029, 3159, 3755, 5114
Tsp45I GTSAC 5 cut(s) 404, 763, 2352, 3219, 4540
TspGWI ACGGA 2 cut(s) 531, 4294
Van91I CCANNNNNTGG 2 cut(s) 57, 565
Vha464I CTTAAG 2 cut(s) 4766, 4826
VneI GTGCAC 1 cut(s) 2874
VpaK11BI GGWCC 1 cut(s) 4268
VspI ATTAAT 1 cut(s) 282
XagI CCTNNNNNAGG 3 cut(s) 3083, 4769, 4998
XbaI TCTAGA 1 cut(s) 1225
XceI RCATGY 5 cut(s) 806, 1507, 1970, 2318, 2801
XmiI GTMKAC 1 cut(s) 1405
XmnI GAANNNNTTC 5 cut(s) 119, 354, 841, 1680, 4952
XspI CTAG 7 cut(s) 1226, 2093, 2607, 2669, 3372, 4176, 4808
ZrmI AGTACT 1 cut(s) 4740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.