Rroxscaffold_5G00386350

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000223
Physical Location & Seq
Forward (+)
65254261 .. 65262691
8431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386350.1

Sequence Viewer

Length: 4296 bp
ATGATGGAAGGTTCAGAAAGCTTCACTGACACTGTGCTTTCTTGGTCCCTTGAAGACATATGTAATGAAAACCTTTACAAGCACCAGGTGGAAAAGATTCCCGAATCAGTTCAACAATTTGGGGCATGTTCATATCCATTATTGGATGAAACTCGAGCTCAGTTGCAATCCAGTATGGAATCCATGCACAGCGCGCCATTTTGTCAAGTAAATGCCATCCAAGAGTCAAAGCCCTATGAAGGAGAAACAAAGCTATATGATATGGAGATTGATGGCTGGAGAAACAGTGATTCTGGCATGGAGTTGCACAAACCCATTCAGGGAGATGTTTTTGTTTTAGCAGATGCTAAACTCGAAACTATATCTGATTTACGAAAGTTGGGGACATGGTGGTCACTTGTAATAGTCACTGAGGTCTCCACAGATAAGAAAGAGGATGATAGAACCTCTCTTTCCCTTAAAGTCAGAGCACCAAAAGAATTTGAAGTCAACAATGGCAATGGAACATCACTGTTTTTGGTCTTCTTAGCAAACATAACTCCTAACTTAAGGATATGGAACGCCATGCACATGTGCGTCAGTTCCAGATGGAAGGTGCTGTTTGGTGATAACTTTATGAAATCATTCAAAAACTTGAAGTCATTCCAAAAGAAGATGTCAGTGCTGAATCTTCTTCTCAAGCTTTCGAGTGGCTGGAGACCCAAGAAACGAAATGTAGACACAATATGTAAAAGCTCTTCGACGACCCTGAAGCAATTTAAGGTTGATGATCTATATATTGTTTTGACAATCGACATTGAAAAGGATTTGAAATACATGCAAGTGTTGAGGATGTGGGACTTATTGCCTGGTCTGTTGGATATCCAAAAATTGGCAGATCGATTGGACTGCATATTTAGTAGATACACGAATCTTTTTGTCAATCTCTGTAGAGAGAAATGTCTTGAGGGTCATTTGGAAGTTCCAGCAAGCTGGCCTCTCTCTTTGGATGTTGTCCGCTATAAAGAAATAAGCAGCACTGAAAGCATGAGTAATTCTGTTGGTGACTCTTCTGCGAATGGAAGCTGTGTTGAGAATTCAAAGGTCAATGAGAGCTTGTTGCTGATGAAGTTTTACTCCTTATCATCTGGGGTAGTGAACTTCTTGTTATCTGACAGAGAGGACAGCAAAGTGGATCTCCCTTTTGAAGTTACTGACCAAGAAAGGGAGGCTGTTCTTTATGATAGAAGTATATTTATACTAGGACGGTCAGGCACTGGAAAGACAACAGTTTTGACTACAAAGTTATATCAGAAAGAACAGCAGCACCATATGGTTGATGAAGGTTTCTATGATGTTGAAAGCAATGCAGTCGGGCATGCTGGTCTGCATAATAAAGCTGGACAGAGTTCTTCTTCTGCATTAAATAAAGGGACTGTGTTACGCCAGCTTTTTGTGACTGTGAGTCCTAAACTCTGTTTTGCCGTCAAACAACATTTTTTACGCTTGAAAAGTTTTGCTTCCGGCAGAAGCAGTTTGGTTGATATGGATGATTTTGATGATGAGGAAGCTCAATTTAGGGGTATCCCAGATTCTTTTTGTGATATTCCTCCAAAATGTTATCCTCTTTTCGTAACATTTCATAAGTTCTTGATGATGCTTGATGGAAGTCTGAGTAATTCATACTTTGAAAGATTCCCAAACATCACAAAACTACCTCAAGGTCGACAGCGAATATCAAGAGCTAATGTGCTGCAGACCTTCCTCACCACCAAGGAGGTCACATATGCAAGGTTTAGCTCATCATATTGGCCTCATTTTGATGTTCAGTTGACAAAGAAGCTTGATGCTTCACGAGTCTTTACTGAGATTATTTCTCATATCAAAGGTGGTCTTAGAGCCTTGGAAGCAGGTGAAGGAAAACTCAGTCAGTTGGATTATGTGCAAATGTCAGAGGGCCGGGCTTCCAGTTTAAGCCAGCAAAAGAGAGAGATAATATATGATATATTTCAGGTCTATGAGAAAATGAAAATGAGAAATGGTGAGTTTGATATTGCTGATTTTGTAAATGATATTCACCGTCGGCTCAAATGTGAAAAATACAAGGGTGATGAAATTGACTTTGTGTACATTGATGAGGTCCAGGATCTTGCCATGAGCCAAATTGCATTGTTTAAACATATCTGCAGCAATGTTGAAGAGGGTTTCGTTTTTTCTGGAGATACAGCCCAGACTATAGCAAGGGGTATTGATTTTAGATTCCAAGATATACGACATCTCTTTTACAAGAAGTTTGTATTGGAATCAAGAAACAAAAAACATGACAATAGGAAAGAAAAAGGACAGATCTCAAACATCTTTCAGTTGACACAAAACTTCCGAAGCCATGATGGGATTCTGAAGTTGTTGCAGAGCATTGTTGAACTGATTTATCATTTTTTCCCCCTCTGTATTGATGTCTTAGAACCAGAAACGAGTATGATATATGGGGAAGCTCCGATTCTACTCTATTCTGGAAATGATGAAAAACTATTCAAAAAAATTCTAGGAAGTAGTGGGATTATTGCCGGAAATATGGGTGGCTTTGGTGCGGATCAAGTTATTTTGGTCCGCGATGATTGTTCTCGAGAAGAAATCTCTAACTTCATTGGGAAGCAAGCTCTAGTTCTTACTATAGTGGAGTGCAAGGGCCTAGAATTTGAGGATGTACTCCTATACAAATTTTTTGGATCATCACCACTGAAAAATCAATGGAGGGTGCTATATGAATTCATGCAAGAACAAAATTTTCTGGACTCCACATTACCTGAACGCTTTCCAAGTTTTGATGATGCCAAACACAGGATGTTATGCTCCGAACTAAAGCAGTTATACGTGGCTGTCTCTCGTACAAGACAGAGACTCTGGATATACGAGAATGTTGAAGAGCTTTCCAACCCAATTTTTGACTATTGGAAAAGGCAACATCTTGTACAACCTAGAGAACTAGACGATTCACTTGCACAAATTATGCAAGTTGCAAGCACTTCAGGAGACTGGAGATCACGTGGCATCAAGCTATATCATGAGCATAACTACAAAATGGCCAGAATGTGCTTTCATAAAGCTGGTGATACATTTTGGGAAAGACGGTCTGAAGCTGCTGAACTTAAAGTCAAGGCAGACCAGATGCGCACTTCTAGTCCTGGAGAGGCAAATGCACTCCTTAGGAAAGCAGCTTCAATTTTTGAAGCTATAGGCCTGTTTGTACCTTCTGCCAGTTGCTTTTATGATTTGGGCGAGTATGAACGAGCAGGTACAATTTATTTGTATAAATGTGGCGAACTGGAACTGGAAAATGCTGGGGAGTGCTTTTATCTTGCTGAAAATTATGTGCTTGCTGCAGATGTGTATGCTAGAGGAAACTTTTTCTTTCAGAGTATTTCTTCTTGTTTACGAGGAAATATATTTCACAGAGGTTTGGAATACATAAAATATTGGAGACAACATGCAAGAGACGAGTATGATCTGGCCAGACAAGGAGATGAGACTCATGAAATGGAATTGGAATTTCTTGAGCATTGCGCATTTCACTATTATGAGGGAAAAGATGACAGATCAAGGATGAAAGTTCTTAGAGATAGGATGCTTTCTACTCAAAAGTTTTTGAATGCTCAGATTTCATCACGCACTTCAAAATATTTGTGGGAAGAAAAACTGCCTGGTGATCTTAAAAGGCCTTCAAACAAAAAACATGAGAAGCAGGTTTCTATTGATTCACTGGTCTACTTCTGGAATTCTTGGAAGGATACAGTTGTACACCTGATTGAATATCTTGGATCGATAGATGTTAATGATTACATAAGTCATGGCGACTTCTTTCTGAATTATTTAGGGGTGTGGAGGCATTTTCATGATGGTCTAAATCCGCTCTATCTTTCACTCATTTATGATATTGACTGGGTGAGAAGTATTGACAAGAGATCTTTCCCAAGAAATGAGGAGTCGGTCCCCATTGATGTTCACCAGCTTGTCTTGGATGCTCAGAGTTATTGGAGTTCAGAGATGCTTTCTCTTGGGATTAAGGTTTTGGAGAAGCTTGAAGTGCTTTACAACTTCACAATCAATAATTCTGATTCAGTGTTCTGCCAGAGCCGGTCTCTTATCCATATATACGAGGTTGCAATATATCTTCTGGAATCCAAATTTCTGAAGATGACTCATTATCATGCTGAGACACTCCACAGATTTGTTACATTGTCGACTGAGAATTTTGTCTCTTACATATTTCCTTTAGATTGGATGAGGTCATCAAGAGAGGATATGATTTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1431

Amino Acids

165.61

Weight (kDa)

5.93

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 66 - 192 7.7e-40 Domain of unknown function (DUF6469)
UvrD-helicase PF00580 644 - 739 7.7e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1880
AasI GACNNNNNNGTC 1 cut(s) 391
Acc16I TGCGCA 2 cut(s) 3152, 3544
Acc36I ACCTGC 3 cut(s) 1880, 3263, 3712
AccB7I CCANNNNNTGG 1 cut(s) 871
AccBSI CCGCTC 1 cut(s) 3889
AccI GTMKAC 4 cut(s) 717, 1705, 3744, 4220
AccII CGCG 2 cut(s) 194, 2592
AciI CCGC 4 cut(s) 997, 2570, 2590, 3887
AclWI GGATC 5 cut(s) 1182, 2133, 2580, 2716, 3805
AcoI YGGCCR 2 cut(s) 3063, 3489
AcuI CTGAAG 5 cut(s) 770, 2399, 2991, 3135, 4190
AcvI CACGTG 1 cut(s) 3026
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 7 cut(s) 2108, 2688, 2869, 2952, 3228, 3277, 3777
AfiI CCNNNNNNNGG 5 cut(s) 239, 320, 871, 1204, 2820
AflII CTTAAG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 570
AhdI GACNNNNNGTC 1 cut(s) 1443
AjnI CCWGG 5 cut(s) 84, 847, 2121, 3163, 3679
AjuI GAANNNNNNNTTGG 2 cut(s) 2261, 2293
Alw21I GWGCWC 2 cut(s) 160, 472
AlwI GGATC 5 cut(s) 1182, 2133, 2580, 2716, 3805
AlwNI CAGNNNCTG 1 cut(s) 1256
Ama87I CYCGRG 2 cut(s) 153, 2604
AoxI GGCC 8 cut(s) 974, 1790, 1936, 2668, 3063, 3217, 3489, 3695
ApeKI GCWGC 7 cut(s) 1014, 1303, 1732, 2166, 3119, 3194, 3359
ArsI GACNNNNNNTTYG 2 cut(s) 1255, 1287
Asp700I GAANNNNTTC 5 cut(s) 96, 108, 623, 641, 2793
AspLEI GCGC 4 cut(s) 194, 196, 3153, 3545
AspS9I GGNCC 6 cut(s) 45, 1936, 2119, 2587, 2668, 3967
AsuC2I CCSGG 1 cut(s) 1940
AvaI CYCGRG 2 cut(s) 153, 2604
AvaII GGWCC 4 cut(s) 45, 2119, 2587, 3967
AxyI CCTNAGG 1 cut(s) 3185
BaeI ACNNNNGTAYC 2 cut(s) 3759, 3792
BalI TGGCCA 2 cut(s) 3065, 3491
BanII GRGCYC 1 cut(s) 160
BarI GAAGNNNNNNTAC 2 cut(s) 721, 753
BauI CACGAG 1 cut(s) 1833
BbrPI CACGTG 1 cut(s) 3026
BbsI GAAGAC 2 cut(s) 60, 514
Bbv12I GWGCWC 2 cut(s) 160, 472
BbvI GCAGC 7 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346
BccI CCATC 6 cut(s) 224, 266, 582, 1637, 2363, 3869
BceAI ACGGC 1 cut(s) 1448
BcgI CGANNNNNNTGC 6 cut(s) 870, 904, 1333, 1367, 2960, 2994
BciT130I CCWGG 5 cut(s) 86, 849, 2123, 3165, 3681
BciVI GTATCC 2 cut(s) 1574, 3760
BcnI CCSGG 1 cut(s) 1940
BfaI CTAG 8 cut(s) 1241, 2525, 2642, 2672, 2958, 2966, 3159, 3375
BfmI CTRYAG 7 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360
BfrI CTTAAG 1 cut(s) 547
BfuAI ACCTGC 3 cut(s) 1880, 3263, 3712
BfuI GTATCC 2 cut(s) 1574, 3760
BglII AGATCT 2 cut(s) 2325, 3941
BisI GCNGC 7 cut(s) 1015, 1304, 1733, 2167, 3120, 3195, 3360
BlsI GCNGC 7 cut(s) 1016, 1305, 1734, 2168, 3121, 3196, 3361
Bme1390I CCNGG 6 cut(s) 86, 849, 1940, 2123, 3165, 3681
Bme18I GGWCC 4 cut(s) 45, 2119, 2587, 3967
BmeRI GACNNNNNGTC 1 cut(s) 1443
BmeT110I CYCGRG 2 cut(s) 153, 2604
BmgT120I GGNCC 6 cut(s) 45, 1936, 2119, 2587, 2668, 3967
BmiI GGNNCC 2 cut(s) 47, 3969
BmrFI CCNGG 6 cut(s) 86, 849, 1940, 2123, 3165, 3681
BmrI ACTGGG 1 cut(s) 3928
BmsI GCATC 9 cut(s) 334, 1626, 1816, 2800, 3039, 3138, 3594, 3988, 4014
BmuI ACTGGG 1 cut(s) 3928
BpiI GAAGAC 2 cut(s) 60, 514
BplI GAGNNNNNCTC 2 cut(s) 4102, 4134
BpmI CTGGAG 5 cut(s) 298, 715, 2217, 3037, 3186
BpuEI CTTGAG 4 cut(s) 662, 965, 1683, 3554
BpuMI CCSGG 1 cut(s) 1940
Bsa29I ATCGAT 2 cut(s) 880, 3800
BsaAI YACGTR 2 cut(s) 2854, 3026
BsaI GGTCTC 3 cut(s) 421, 691, 4122
BsaJI CCNNGG 2 cut(s) 1752, 1881
BsaXI ACNNNNNCTCC 4 cut(s) 271, 301, 3010, 3040
Bsc4I CCNNNNNNNGG 5 cut(s) 239, 320, 871, 1204, 2820
Bse118I RCCGGY 1 cut(s) 4113
Bse1I ACTGG 9 cut(s) 171, 1261, 1947, 3020, 3237, 3309, 3315, 3744, 3923
Bse21I CCTNAGG 1 cut(s) 3185
Bse3DI GCAATG 4 cut(s) 505, 1351, 2176, 3538
BseBI CCWGG 5 cut(s) 86, 849, 2123, 3165, 3681
BseCI ATCGAT 2 cut(s) 880, 3800
BseDI CCNNGG 2 cut(s) 1752, 1881
BseLI CCNNNNNNNGG 5 cut(s) 239, 320, 871, 1204, 2820
BseMI GCAATG 4 cut(s) 505, 1351, 2176, 3538
BseMII CTCAG 9 cut(s) 173, 402, 1643, 1836, 1918, 3647, 4016, 4182, 4215
BseNI ACTGG 9 cut(s) 171, 1261, 1947, 3020, 3237, 3309, 3315, 3744, 3923
BsePI GCGCGC 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 3974
BseXI GCAGC 7 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346
BseYI CCCAGC 1 cut(s) 3320
Bsh1236I CGCG 2 cut(s) 194, 2592
BshFI GGCC 8 cut(s) 976, 1792, 1938, 2670, 3065, 3219, 3491, 3697
BshVI ATCGAT 2 cut(s) 880, 3800
BsiHKAI GWGCWC 2 cut(s) 160, 472
BsiHKCI CYCGRG 2 cut(s) 153, 2604
BsiSI CCGG 4 cut(s) 1503, 1939, 2547, 4114
BslFI GGGAC 5 cut(s) 31, 397, 851, 1426, 3953
BslI CCNNNNNNNGG 5 cut(s) 239, 320, 871, 1204, 2820
BsmBI CGTCTC 1 cut(s) 3468
BsmFI GGGAC 5 cut(s) 31, 397, 851, 1426, 3953
BsmI GAATGC 1 cut(s) 3634
BsnI GGCC 8 cut(s) 976, 1792, 1938, 2670, 3065, 3219, 3491, 3697
Bso31I GGTCTC 3 cut(s) 421, 691, 4122
BsoBI CYCGRG 2 cut(s) 153, 2604
Bsp1286I GDGCHC 2 cut(s) 160, 472
Bsp1407I TGTACA 3 cut(s) 2106, 2950, 3775
BspACI CCGC 4 cut(s) 997, 2570, 2590, 3887
BspANI GGCC 8 cut(s) 976, 1792, 1938, 2670, 3065, 3219, 3491, 3697
BspCNI CTCAG 9 cut(s) 172, 403, 1644, 1837, 1917, 3646, 4015, 4183, 4216
BspDI ATCGAT 2 cut(s) 880, 3800
BspFNI CGCG 2 cut(s) 194, 2592
BspHI TCATGA 3 cut(s) 3043, 3511, 3871
BspLI GGNNCC 2 cut(s) 47, 3969
BspMAI CTGCAG 3 cut(s) 1737, 2168, 3364
BspMI ACCTGC 3 cut(s) 1880, 3263, 3712
BspPI GGATC 5 cut(s) 1182, 2133, 2580, 2716, 3805
BspQI GCTCTTC 2 cut(s) 742, 2898
BspTI CTTAAG 1 cut(s) 547
BspTNI GGTCTC 3 cut(s) 421, 691, 4122
BsrBI CCGCTC 1 cut(s) 3889
BsrDI GCAATG 4 cut(s) 505, 1351, 2176, 3538
BsrFI RCCGGY 1 cut(s) 4113
BsrGI TGTACA 3 cut(s) 2106, 2950, 3775
BsrI ACTGG 9 cut(s) 171, 1261, 1947, 3020, 3237, 3309, 3315, 3744, 3923
BssAI RCCGGY 1 cut(s) 4113
BssECI CCNNGG 2 cut(s) 1752, 1881
BssHII GCGCGC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 1833
BssT1I CCWWGG 2 cut(s) 1752, 1881
Bst2BI CACGAG 1 cut(s) 1833
Bst2UI CCWGG 5 cut(s) 86, 849, 2123, 3165, 3681
Bst6I CTCTTC 4 cut(s) 742, 1054, 2172, 2898
BstAFI CTTAAG 1 cut(s) 547
BstAUI TGTACA 3 cut(s) 2106, 2950, 3775
BstBAI YACGTR 2 cut(s) 2854, 3026
BstC8I GCNNGC 9 cut(s) 194, 970, 974, 1359, 1427, 1958, 2637, 3001, 3357
BstFNI CGCG 2 cut(s) 194, 2592
BstHHI GCGC 4 cut(s) 194, 196, 3153, 3545
BstMWI GCNNNNNNNGC 4 cut(s) 193, 1023, 1886, 4063
BstNI CCWGG 5 cut(s) 86, 849, 2123, 3165, 3681
BstNSI RCATGY 5 cut(s) 129, 574, 820, 1361, 3470
BstSCI CCNGG 6 cut(s) 84, 847, 1938, 2121, 3163, 3679
BstSFI CTRYAG 7 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360
BstUI CGCG 2 cut(s) 194, 2592
BstV1I GCAGC 7 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346
BstV2I GAAGAC 2 cut(s) 60, 514
BstX2I RGATCY 4 cut(s) 1174, 2125, 2325, 3941
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 4 cut(s) 1174, 2125, 2325, 3941
Bsu15I ATCGAT 2 cut(s) 880, 3800
Bsu36I CCTNAGG 1 cut(s) 3185
BsuI GTATCC 2 cut(s) 1574, 3760
BsuRI GGCC 8 cut(s) 976, 1792, 1938, 2670, 3065, 3219, 3491, 3697
BsuTUI ATCGAT 2 cut(s) 880, 3800
BtgZI GCGATG 1 cut(s) 2607
BveI ACCTGC 3 cut(s) 1880, 3263, 3712
Cac8I GCNNGC 9 cut(s) 194, 970, 974, 1359, 1427, 1958, 2637, 3001, 3357
CaiI CAGNNNCTG 1 cut(s) 1256
CciI TCATGA 3 cut(s) 3043, 3511, 3871
CfoI GCGC 4 cut(s) 194, 196, 3153, 3545
Cfr10I RCCGGY 1 cut(s) 4113
Cfr13I GGNCC 6 cut(s) 45, 1936, 2119, 2587, 2668, 3967
ClaI ATCGAT 2 cut(s) 880, 3800
CseI GACGC 1 cut(s) 565
CsiI ACCWGGT 1 cut(s) 84
Csp6I GTAC 7 cut(s) 2107, 2687, 2868, 2951, 3227, 3276, 3776
CviQI GTAC 7 cut(s) 2107, 2687, 2868, 2951, 3227, 3276, 3776
DraI TTTAAA 1 cut(s) 2155
DraIII CACNNNGTG 1 cut(s) 88
DrdI GACNNNNNNGTC 1 cut(s) 391
DriI GACNNNNNGTC 1 cut(s) 1443
DseDI GACNNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 3063, 3489
Eam1104I CTCTTC 4 cut(s) 742, 1054, 2172, 2898
Eam1105I GACNNNNNGTC 1 cut(s) 1443
EarI CTCTTC 4 cut(s) 742, 1054, 2172, 2898
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 1752, 1881
Eco147I AGGCCT 2 cut(s) 3219, 3697
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 3 cut(s) 421, 691, 4122
Eco32I GATATC 1 cut(s) 862
Eco47I GGWCC 4 cut(s) 45, 2119, 2587, 3967
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 5 cut(s) 770, 2399, 2991, 3135, 4190
Eco72I CACGTG 1 cut(s) 3026
Eco81I CCTNAGG 1 cut(s) 3185
Eco88I CYCGRG 2 cut(s) 153, 2604
EcoICRI GAGCTC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 2668
EcoRI GAATTC 3 cut(s) 1075, 2747, 3754
EcoRII CCWGG 5 cut(s) 84, 847, 2121, 3163, 3679
EcoRV GATATC 1 cut(s) 862
EcoT14I CCWWGG 2 cut(s) 1752, 1881
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 1752, 1881
Esp3I CGTCTC 1 cut(s) 3468
FalI AAGNNNNNCTT 4 cut(s) 1483, 1515, 1857, 1889
FaqI GGGAC 5 cut(s) 31, 397, 851, 1426, 3953
FauNDI CATATG 3 cut(s) 59, 1311, 1765
FblI GTMKAC 4 cut(s) 717, 1705, 3744, 4220
Fnu4HI GCNGC 7 cut(s) 1015, 1304, 1733, 2167, 3120, 3195, 3360
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 7 cut(s) 1015, 1304, 1733, 2167, 3120, 3195, 3360
FspAI RTGCGCAY 1 cut(s) 3152
FspBI CTAG 8 cut(s) 1241, 2525, 2642, 2672, 2958, 2966, 3159, 3375
FspI TGCGCA 2 cut(s) 3152, 3544
GlaI GCGC 4 cut(s) 193, 195, 3152, 3544
GluI GCNGC 7 cut(s) 1015, 1304, 1733, 2167, 3120, 3195, 3360
GsaI CCCAGC 1 cut(s) 3324
GsuI CTGGAG 5 cut(s) 298, 715, 2217, 3037, 3186
HaeIII GGCC 8 cut(s) 976, 1792, 1938, 2670, 3065, 3219, 3491, 3697
HapII CCGG 4 cut(s) 1503, 1939, 2547, 4114
HgaI GACGC 1 cut(s) 565
HhaI GCGC 4 cut(s) 194, 196, 3153, 3545
Hin6I GCGC 4 cut(s) 192, 194, 3151, 3543
HinP1I GCGC 4 cut(s) 192, 194, 3151, 3543
HincII GTYRAC 5 cut(s) 490, 1706, 1812, 2346, 4221
HindII GTYRAC 5 cut(s) 490, 1706, 1812, 2346, 4221
HindIII AAGCTT 4 cut(s) 19, 680, 1820, 4055
HpaII CCGG 4 cut(s) 1503, 1939, 2547, 4114
Hpy99I CGWCG 2 cut(s) 745, 2064
HpyAV CCTTC 8 cut(s) 233, 586, 1316, 1750, 1889, 3240, 3708, 3757
HpyCH4IV ACGT 2 cut(s) 2853, 3025
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 1023, 1886, 4063
HpySE526I ACGT 2 cut(s) 2853, 3025
HspAI GCGC 4 cut(s) 192, 194, 3151, 3543
LguI GCTCTTC 2 cut(s) 742, 2898
LmnI GCTCC 2 cut(s) 2479, 2837
Lsp1109I GCAGC 7 cut(s) 1026, 1315, 1719, 2178, 3106, 3206, 3346
LweI GCATC 9 cut(s) 334, 1626, 1816, 2800, 3039, 3138, 3594, 3988, 4014
MabI ACCWGGT 1 cut(s) 84
MaeI CTAG 8 cut(s) 1241, 2525, 2642, 2672, 2958, 2966, 3159, 3375
MaeII ACGT 2 cut(s) 2853, 3025
MaeIII GTNAC 9 cut(s) 393, 406, 1043, 1189, 1419, 1435, 1612, 1759, 4210
MbiI CCGCTC 1 cut(s) 3889
MflI RGATCY 4 cut(s) 1174, 2125, 2325, 3941
MhlI GDGCHC 2 cut(s) 160, 472
MlsI TGGCCA 2 cut(s) 3065, 3491
MluNI TGGCCA 2 cut(s) 3065, 3491
MlyI GAGTC 9 cut(s) 233, 1040, 1453, 1845, 2768, 2874, 3502, 3971, 4171
MmeI TCCRAC 3 cut(s) 837, 1893, 2937
Mox20I TGGCCA 2 cut(s) 3065, 3491
MroXI GAANNNNTTC 5 cut(s) 96, 108, 623, 641, 2793
MscI TGGCCA 2 cut(s) 3065, 3491
MslI CAYNNNNRTG 6 cut(s) 569, 821, 1800, 3555, 3870, 4185
Msp20I TGGCCA 2 cut(s) 3065, 3491
MspCI CTTAAG 1 cut(s) 547
MspI CCGG 4 cut(s) 1503, 1939, 2547, 4114
MspR9I CCNGG 6 cut(s) 86, 849, 1940, 2123, 3165, 3681
MssI GTTTAAAC 1 cut(s) 2155
Mva1269I GAATGC 1 cut(s) 3634
MvaI CCWGG 5 cut(s) 86, 849, 2123, 3165, 3681
MvnI CGCG 2 cut(s) 194, 2592
MwoI GCNNNNNNNGC 4 cut(s) 193, 1023, 1886, 4063
NciI CCSGG 1 cut(s) 1940
NdeI CATATG 3 cut(s) 59, 1311, 1765
NlaIV GGNNCC 2 cut(s) 47, 3969
NmuCI GTSAC 5 cut(s) 393, 406, 1043, 1435, 1759
NsbI TGCGCA 2 cut(s) 3152, 3544
NspI RCATGY 5 cut(s) 129, 574, 820, 1361, 3470
PaeI GCATGC 1 cut(s) 1361
PaeR7I CTCGAG 2 cut(s) 153, 2604
PagI TCATGA 3 cut(s) 3043, 3511, 3871
PaqCI CACCTGC 1 cut(s) 1880
PauI GCGCGC 1 cut(s) 192
PceI AGGCCT 2 cut(s) 3219, 3697
PciI ACATGT 1 cut(s) 570
PciSI GCTCTTC 2 cut(s) 742, 2898
PctI GAATGC 1 cut(s) 3634
PdmI GAANNNNTTC 5 cut(s) 96, 108, 623, 641, 2793
PflMI CCANNNNNTGG 1 cut(s) 871
PfoI TCCNGGA 2 cut(s) 2121, 3163
PkrI GCNGC 7 cut(s) 1016, 1305, 1734, 2168, 3121, 3196, 3361
PleI GAGTC 9 cut(s) 232, 1040, 1452, 1844, 2768, 2874, 3502, 3970, 4171
PmaCI CACGTG 1 cut(s) 3026
PmeI GTTTAAAC 1 cut(s) 2155
PmlI CACGTG 1 cut(s) 3026
PpsI GAGTC 9 cut(s) 232, 1040, 1452, 1844, 2768, 2874, 3502, 3970, 4171
Ppu21I YACGTR 2 cut(s) 2854, 3026
PscI ACATGT 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 5 cut(s) 84, 847, 2121, 3163, 3679
PspCI CACGTG 1 cut(s) 3026
PspFI CCCAGC 1 cut(s) 3320
PspGI CCWGG 5 cut(s) 84, 847, 2121, 3163, 3679
PspN4I GGNNCC 2 cut(s) 47, 3969
PspPI GGNCC 6 cut(s) 45, 1936, 2119, 2587, 2668, 3967
PspXI VCTCGAGB 1 cut(s) 153
PstI CTGCAG 3 cut(s) 1737, 2168, 3364
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 4 cut(s) 1174, 2125, 2325, 3941
PteI GCGCGC 1 cut(s) 192
RsaI GTAC 7 cut(s) 2108, 2688, 2869, 2952, 3228, 3277, 3777
RsaNI GTAC 7 cut(s) 2107, 2687, 2868, 2951, 3227, 3276, 3776
RseI CAYNNNNRTG 6 cut(s) 569, 821, 1800, 3555, 3870, 4185
SacI GAGCTC 1 cut(s) 160
SalI GTCGAC 2 cut(s) 1704, 4219
SapI GCTCTTC 2 cut(s) 742, 2898
SatI GCNGC 7 cut(s) 1015, 1304, 1733, 2167, 3120, 3195, 3360
Sau96I GGNCC 6 cut(s) 45, 1936, 2119, 2587, 2668, 3967
SchI GAGTC 9 cut(s) 233, 1040, 1453, 1845, 2768, 2874, 3502, 3971, 4171
ScrFI CCNGG 6 cut(s) 86, 849, 1940, 2123, 3165, 3681
SduI GDGCHC 2 cut(s) 160, 472
SexAI ACCWGGT 1 cut(s) 84
SfaNI GCATC 9 cut(s) 334, 1626, 1816, 2800, 3039, 3138, 3594, 3988, 4014
SfcI CTRYAG 7 cut(s) 928, 1733, 2164, 2214, 2652, 3213, 3360
Sfr274I CTCGAG 2 cut(s) 153, 2604
SinI GGWCC 4 cut(s) 45, 2119, 2587, 3967
SlaI CTCGAG 2 cut(s) 153, 2604
SmiMI CAYNNNNRTG 6 cut(s) 569, 821, 1800, 3555, 3870, 4185
SmlI CTYRAG 7 cut(s) 153, 547, 677, 944, 1698, 2604, 3533
SmoI CTYRAG 7 cut(s) 153, 547, 677, 944, 1698, 2604, 3533
SphI GCATGC 1 cut(s) 1361
SseBI AGGCCT 2 cut(s) 3219, 3697
SsiI CCGC 4 cut(s) 997, 2570, 2590, 3887
SspI AATATT 2 cut(s) 3455, 3659
SspMI CTAG 8 cut(s) 1241, 2525, 2642, 2672, 2958, 2966, 3159, 3375
SstI GAGCTC 1 cut(s) 160
StuI AGGCCT 2 cut(s) 3219, 3697
StyD4I CCNGG 6 cut(s) 84, 847, 1938, 2121, 3163, 3679
StyI CCWWGG 2 cut(s) 1752, 1881
TaiI ACGT 2 cut(s) 2856, 3028
TaqII GACCGA 1 cut(s) 3955
TatI WGTACW 4 cut(s) 2106, 2686, 2950, 3775
TseFI GTSAC 5 cut(s) 393, 406, 1043, 1435, 1759
TseI GCWGC 7 cut(s) 1014, 1303, 1732, 2166, 3119, 3194, 3359
Tsp45I GTSAC 5 cut(s) 393, 406, 1043, 1435, 1759
Van91I CCANNNNNTGG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 547
VpaK11BI GGWCC 4 cut(s) 45, 2119, 2587, 3967
XceI RCATGY 5 cut(s) 129, 574, 820, 1361, 3470
XhoI CTCGAG 2 cut(s) 153, 2604
XmiI GTMKAC 4 cut(s) 717, 1705, 3744, 4220
XmnI GAANNNNTTC 5 cut(s) 96, 108, 623, 641, 2793
XspI CTAG 8 cut(s) 1241, 2525, 2642, 2672, 2958, 2966, 3159, 3375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.