Rh2AG265000

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000223
Physical Location & Seq
Reverse (-)
30905492 .. 30917714
12223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG265000.1

Sequence Viewer

Length: 7851 bp
ATGGTAATATATGCTTTTGTTTTGAATGCAGATAACAAAAGCAAGAGAGTTTTGAGTCAGACAAGAAAAAGCAAGGAGATGATGGAAGGTTCACACAAGAAAAAGCCTAAAAAACCCAACCTTTTGGCTGAGACTGTGTTTTCTTGGTCCCTTGATAACATTTTCAATGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCACTCTGTGGAGCATTACTTCGGGTGTTATCTGTATCCTTTATTGGAAGAAACAAGAGCACAGGTCCATGCAAGTATGGAAACTATTTACAGAGCACCATTTGCTAAAGTAGTTGCTTTTGAAAAAGCGAAGCCATATGGAACAAAGCTATATGATATCAAGGTTGATTATTGGCGAAACAGGTTAAATGACCGTGGCAAAGAGCCATATGAAACTTTGCCAGGTGATCTTTTTGTTTTGGCAAATGCTAAACCTGAAAGTTTTTCAGATTTACAAAGGGTAGGGAGGTCATGGGCTTTTGCATCAGTCACTAAAGTCTCAGACAATGAGAACGAGGATGAAAGTACTTCTCTTTATTTTAAAATCAAGGCTTCCAAAGAACTTGAAGTTGTAAAGGGTTCGGCATCACTGTTTATGGTTTTTCTAGTGAACTTAATCCCGAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAACATTATCAAGGAAGTTCTGTGCACTGATTCTGTGGCTCATGAAAATCTCTGCTCTGAAAAGAATAATGACATCGTGAATAAGGGGTTAGTTCAGAGTTTATCATCTGGTCTGAATGAATCCCAAACAGAAACTGTTTTAGCCTGCCTTGAAATGCTGCATTGTCGTGAAAAGTCTGCTGTTGAACTAATTTGGGGTCCTCCTGGTACTGGAAAAACTAAAACAATTGCGACTCTACTTGTAACCTTGTTACAGATGAATTGTAGGACTCTTGTTTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCATGTTGTAAAGATGTTGACTGAAGTAGAGTCCAGTTCTTTGTTTTGTTCTTTTGGAGAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAGAGTTGGCTCAGACATTGAAGAGATATATCTAGATTACCGTCTCAAAAGGCTTTCTGAGTGCTTAGGACCACAGACTGGCTGGAGGCATTGCTTTGCTTCCATGATAGATTTTCTTGAAGATTGTGTTTCTCACTACCATAACTTTTTGGTAAATGAGTTAACCATAGAGAAGGAACCAAAGACCTTAGGTCAAATGAAAGGAAAAGAAAGCAAGTGTGACACTCAAGTTGGCGAGGAGAAGTGCAAATCATTTCTGGAATTTATTAGAGACAGATTTGTTTCTAATGCATCACCTCTAAGAAATTGTATTTCTATCTTATGTACCCACATGGCGAAAAATTACATTTGTGGAGATAATGTCCAAAACATGATTATGCTTATCAACTTGGTTGATTCCTTTGAATCATTGTTGTTTCAAAATAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCTTGTTCTGACATTGACGACATATGGGAGACATATGCTGATAACTCATTTCTTCTATTCATGAATAGAAGAGAATGCCTTAAAGTCTTGCACACTCTGTTTGACTCCCTTAGTGAACTTCACATTCCGAATTTTGTGAAGCAAGAGTCTCTAATGGAATTTTGTTTTCAAAAAGCCTCCTTGATATTTTGCACTGCATCCAGCTCATATAAATTGCATAAAGTGGCAATGGAGCCGTTGAGCGTTGTTGTTATTGATGAAGCTGCACAATTGAAAGAGTCTGAATCAACAATCCCCCTTCAACTATGGGGTGTGAAGCATGCTGTTCTAGTTGGGGATGAATGTCAGTTACCAGCTACAGTGAAGAGCATTGTATCTGATGAAGCAGGTTTTGCAAGAAGTTTGTTCGAGAGGTTAAGCTCATTTGGTCGCTCGAAACACCTTCTAAATATGCAATACAGGATGCATCCATCAATAAGTTTCTTGCCAAATTCTAGATTCTACAATAACATGATCCTGGATGCTCCAAATCTTAAAAGTAGAAGCCAGAAGAAACACTATCTTAAAGGGTCAATGTTTGGTCCTTATTCTTTCATCAATGTAATTGGTGGACGAGAAGAGAAGGATGAGGATGGACGTAGTCGAAAGAATATGGTGGAGGTTGCTATTGTTTCACAAATACTGCGGAATCTCTACAAAAAATGGGTTGAATCAAAGCAGAAACTCAGTATTGGTATAGTATCTCCATATGCTGCTCAAGTAGTCGCAATCGAGGACAAACTTGCACAGAAGTATAATAATCTTGATGGCTTTACAGTGAAGGTAAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTGTCCACTGTACGATCCAATTATCAGCGGTCACTTGAGTTTGTTTCAAAACCGCAAAGAATTAATGTTGCTCTTACAAGGGCTAGGCACTGTCTTTGGATTTTGGGGAATGAAAAAATTCTATGTGATAATGAGTCTGTTTGGAAGGCTGTGGTCCTTGATGCCAAAAATCGCCATTGCTTTTTTAATGCGGATGAAGACAAGGATCTAGCCAAGGCCATATTGGAGGTTAAGAAACAGTATGACCAACTAGATGATTTGCTTGATGCTGACAGTATACTTTTCAGAAGTGCTAGATGGAAGGTGCTTTTTAGTGATAACTTTTTTAAGTCATTCAAGAAACTGACGTCAGTCCGTTTGAAGAGGTCGGTACTAAATGTTCTACTGAAGCTTGCCAGTGGCTGGAGACCAAAGAAGAAGAGTTCAGAAATGATTTGTGGTAGCTCTTCACTGATCTTGAAGAAATTTAAGGTTGAAAATCTGTATGTTGTTTGTACAACAGACATTGCAAAGGACTCGAGATACATTCAAGTATTGAAGATTTGGGACATATTGCCTATAGGGGATGTCCCAACTTTGGTCAATCGCCTGGACCGTATAATGAACAAATATACTGATGCTTTCATTAATCTCTGCAAGGAGAAATGTCTTGAGGGTGATTTGGAAATTCCAAAGAGCTGGCCACCTTCTTTGGAGTTTTTGCGGTTTAAGGATCTTAGCTTCACTAAAATCCAGAATGATTTTGTTGGTGACACATGTGATGGTAGAAGCTACGCTGAGAATTCACGGGTCAGCGAAAGTTTGTTGCTGATGAAGTTCTACTCATTATCATCTGGGGTAGTAAACCACTTGCTGTCTGATTGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACGGACCAAGAAATGGAGATTATCCTTCATCATAGAAGTTCCTTCATAGTTGGAAGGTCAGGAACTGGGAAAACCACTGTTTTAACAATGAAATTATTTCAGAAAGAACAGTGGCACCAGTTGGCAGTGCAAGGATGCTATACTAGTCAAAGCAGCAAGATTGAAAAGAGTTCAGCAGCTACTGAGGAGAAAGTTCTACACCAGCTTTTCGTTACAGTGAGTCCTAAGCTGTGTTATGCCATCAAGCAACATGTCTTACACTTGAAAAGTTTTGCATGTGGTGGAAGTCATTCGACTGAAAGCAGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTCAACGATATCCAAGATTCATTTCATGAAATTCCTCCCCATTCTTACCCTCTTTTCATCACATTTCATAAGTTCTTGATTATGCTTGATGGAACACTGAGCAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGCTTACTCATGGTCATCTACGGAATTCAAGATCAGCTGCCTTGCATACTTTTATAAGAACAAAGGAAGTCAGTTATGAGAGGTTTAGTTCATTATACTGGCCGCATTTTAATACCGATTTAACAAAGAGGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCCCATATAAAAGGTGGCTTGGGAGCTATAGAAGCATGTGATGGTAAACTCAGTCGAGAGGATTATGTACAACTGTCAGAGATCCGAGTTTCCATTCTAACCAGACAAGAGAGAGAAGTTATATATGATATTTTTCAGATGTATGAAAAAATGAAGATGGAAAATGGGGAATTTGATCTTGCTGATTTTGTAATTGATCTTCACCATCGACTGATGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATGTTGATGAGGTTCAAGATCTAACAATGAGTCAAATTGCCTTGTTTAAACATATGTGCCACAATGTTGAAGATGGTTTTGTTTTTTCCGGTGACACTGCACAAACAATTGCAAGGGGTATTGATTTCAGGTTTCAAGAATTACGACATCTATTCTACAAGAAGTTTGTGTTGGAGTCAAGAACCAATAAGCACGATGAAAGTAAAGAAAAAGGCCAAGTCTCGGAAATTCTTCACTTGACTCAAAACTTTCGTACCCATGATGGAATCCTCAAGCTATCACAAAGCATTATTGACCTACTATATCATTTCTTTCCCCTTTCCATTGATGTCTTAAAACCTGAAAGTAGTTTGATATTTGGGGAAGCTCCGGTATTGCTTGAATCTGAAGAAAATGAAAATGCAATCGTAAAGATTTTTGGAAATACTGGGAATATAGTTGGATTTGGTGCAGAGCAGGTAATTTTGGTCCGAGATGATAGTGCTCGAAAAGAGATATCCAAATTTATAGGGAAGCATGCTCTTGTTCTAACCATTGTGGAGTGCAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTTGGCTCATCACCTTCAAAAAACCAGTGGAGGGTTATATATGATTACATGAATGAACTAGATTTACTCGACCACACTTTACCCAGGGGCTTTCCTGGTTTCGATGTAGCCAAACACAACATCTTATGCTCTGAACTGAAGCAACTATATGTGGCTGTTACTCGCACGAGACAGAGATTGTGGATTTGTGAAAATATGGAAGAGCTTTCCAAACCAATGTTTGACTACTGGAAGAAGAAATGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGAAATGCAAGTTGCAAGCAGTCCTGAGGAGTGGAAGTCACGTGGCATTAAGCTATATCATGAACACAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCAGGTGACACTTACTGGGAAAGAAGGTCTAAAGCAGCTGGCCTTAAAGCACTTGCTGCCCATATACGAATGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTAACGCTATAGGCAAGGCAGATTCTGCTGCCAGATGTTTTTCTGATCTGGGGGAGTATGAAAAAGCTGCTAGAATATATTTGAAATGTGGGGAGTCTGAACTGAAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGACGATATAAGGAGGCAGCAGATGTCTATGCTAAGGGTAATTTTTTCGCAGAGTGTCTCACTATTTGTGAAAAGGGAAAACTATTTGAACTGGGTTTGCAGTACATCAATAACTGGAAACAACATGCAGAAGAGGACACTGCTATGACTACTAGAGGAAAAGGAATAAATAGAATGCAGCTTGAATTTTTAGAGAGCTGTGCACTTCACTATTATGATTTGAATGATAACAGATCCATGATTAAGTTTATTAAAGCTTTTCCTTCTATAACTTTGGTGCGGGACTTTTTGAAGAGGTTGGATAGCCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCAAACATCGCACAGCTTAAAGGTGAAATTCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAAGCCTCATTGAAAATAATATTCTACGTACTTGCCATGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCTGAAGAAGTTTCCACAACAGGAGGCCCTCTTGGCAAAAGCCAAGTCACTTGCCAAGAATGAGACAGACAACTTTCATGAGTTGGTTTGCACTGAGGTTGATATTTTATTAAATAGGCAGGATAACTTGGCTGTGCTGAAAAATCAATTGAATTCTTCTCAGAGACACCAGAGTATCAGAGGGGAAGTTTTATCTGCTCGAAAAATTTTGGATGCACATCTTTCTCAAAGAACTGATAAGTATATATGGGAAAAAGAATTGATTGGTGATCTTGTAGAGGAGTCAGAAGAGAAAATATCTAAAAATCAGGTTTCCATTGATTCACTGGTATACTTTTGGAATTTTTGGAAGGAGAAGATTCTTCACATATATGAATCTGTGCGATGTTTGGAAACTCCAGAATGCAAGGGATATGTAGAGTGTTGTTTAACTTGCTTAGGTGTGTGTAGGCTATATCATGATCTGAATCCAGTCTATGTGTTGCTCCTCCCTCGTGCTGATTGGGTAAGATGTCTGGAAAGAAGACAATTCCAAAATCACGGGAAGTTGGTCTCCATTGATGGTCAGCAGCTCACTTCAGCTGCTCAGAAATATTGGAGTTCAGAAATGATCTCTGTTGGAATCAAGGTTTTGGAAAAGCTTGAAGCTCTTTACAAGTTCCCTGTCAAGAATTCTGATTCAGCCTTCTTCCAAAGCAAGTCCCTTACCTTGATCTATGATGTTGCAGTGTATCTTGTGGATTCCAAATGTCTGAAGCCTAGGCATCAAGATACCATAACACTACAGAGGTTTGTAACTTTTTCAACTGAGAGTTTGGTGTCTTACATGTTTCCTTTAGATGGGAGGAACTCATTGAGAGAGAGTATGATTTCTCTTAGAAGAACTGATGCTTTAAAGAATGCTCTGAGACAAGTAGTATTTGAATATACCCGGTCAAAGAACAAGCCGTCTTTTGGGGATATTGGAAGGATTGCAATGATCATTCTCGGGACTGGTAAGCTGAATAGCGAACTATATGCTAAGCTTCTCAAAAAGCTAGACTCCCACTCAAATTTGAGGGCCTTCATTATTTTTCTTTGCGAGACTTTTGGACGTGGGGAAATTATTTACGTGAGTATTATGTTGCCATTCCGTAAGGAAGTTAAAGTCAACGGGAGGGTTATTTATGACTACATGTCACCTGGTTGCTTCTTGTATATTCTGGAACGCCTTCTGATTTGGGCAGCTCTTACTCAAGGTTATGTTCTTACTACAAGTTCTTGCTTTATTGAATGGCTGATATCCCAGGATGAAGATAGCCGCTTAAATTTCATTGTGGAAGAAGATCGATCACCTTCTCTCGAAGGCATCCTTGAACTTACGATTAATGACTGTATATCGGAAAAGAAGGATATGATTGAATGGCTTGAAAAGTCTGACAAAAACTGGAAGAAGCATTATCCGCTATACATCCTTCGATTGGTTGTTCTGTTATGTTTGCTTTATGTGAACTTTGGTTTGTCTTTGGATACACTCTTTGACTTTCTGGGGAGGAGCTATGTCGCTGAGCAGTTGCCTTGTGACTTTGTTGACACCCTTAGGAGGGGGTGGAGTCATGATTCCCTTAGGAGGGATGATTCTCCGATTTCAAATGTGAGTGCACTTGCTGCAGCTCTCAAGAAGGATGATAATGATTCGGTGTTTGCAAGTTTTAGGCAGGATTGTAGTTGTACACCTCATAATTCTCTGAGTTCAAATGTGAATGCACTTGCTGCAGCTCTCAAGAAGGTTGATAATGATTTGGTAATTGCAAGTTTTAGGCGGGATTGTTCAAAGTTATGTTGTTCAGATGCCATCTTTATTGACATGACGGCCAACCAGTGCAAGGATGACATACTGAGAAAAATTGTGTCCAAAGCAGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2616

Amino Acids

299.67

Weight (kDa)

6.46

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 104 - 232 7.5e-64 Domain of unknown function (DUF6469)
AAA_11 PF13086 270 - 642 2.3e-34 AAA domain
AAA_19 PF13245 290 - 638 1.8e-06 AAA domain
AAA_12 PF13087 651 - 847 8e-57 AAA domain
AAA_19 PF13245 1116 - 1447 8.4e-06 AAA domain
UvrD-helicase PF00580 1118 - 1444 1.8e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3860
AarI CACCTGC 1 cut(s) 5180
AatII GACGTC 1 cut(s) 2777
Acc36I ACCTGC 4 cut(s) 1934, 4669, 5180, 5849
AccB1I GGYRCC 1 cut(s) 3438
AccB7I CCANNNNNTGG 3 cut(s) 1178, 2829, 3337
AccI GTMKAC 2 cut(s) 2704, 6273
AccII CGCG 1 cut(s) 5423
AclWI GGATC 8 cut(s) 2065, 2436, 2640, 3147, 3315, 4045, 4212, 5670
AcoI YGGCCR 4 cut(s) 3107, 3905, 5166, 7794
AcvI CACGTG 1 cut(s) 5129
AcyI GRCGYC 1 cut(s) 2774
AdeI CACNNNGTG 2 cut(s) 220, 5175
AflIII ACRYGT 5 cut(s) 681, 3182, 3574, 6768, 7116
AhdI GACNNNNNGTC 2 cut(s) 1290, 7117
AhlI ACTAGT 1 cut(s) 3467
AjiI CACGTC 1 cut(s) 7037
AjnI CCWGG 9 cut(s) 433, 898, 2073, 2400, 3015, 4889, 4900, 7123, 7227
AjuI GAANNNNNNNTTGG 4 cut(s) 3675, 3707, 4376, 4408
Alw21I GWGCWC 6 cut(s) 274, 310, 722, 4708, 5647, 7585
Alw44I GTGCAC 3 cut(s) 718, 5643, 7581
AlwI GGATC 8 cut(s) 2065, 2436, 2640, 3147, 3315, 4045, 4212, 5670
AlwNI CAGNNNCTG 5 cut(s) 830, 2829, 3389, 3506, 5333
Ama87I CYCGRG 2 cut(s) 2944, 6929
ApaLI GTGCAC 3 cut(s) 718, 5643, 7581
ArsI GACNNNNNNTTYG 2 cut(s) 5972, 6004
AseI ATTAAT 3 cut(s) 2490, 3054, 7308
Asp700I GAANNNNTTC 7 cut(s) 198, 210, 1074, 2544, 3613, 4452, 7152
AspA2I CCTAGG 1 cut(s) 6701
AsuC2I CCSGG 1 cut(s) 6874
AvaI CYCGRG 2 cut(s) 2944, 6929
AvaII GGWCC 9 cut(s) 147, 277, 893, 1169, 2138, 2581, 3019, 3328, 4690
AvrII CCTAGG 1 cut(s) 6701
AxyI CCTNAGG 4 cut(s) 1288, 5112, 7520, 7547
BaeGI GKGCMC 4 cut(s) 722, 979, 5647, 7585
BaeI ACNNNNGTAYC 4 cut(s) 4459, 4492, 6100, 6133
BalI TGGCCA 2 cut(s) 3109, 5168
BanI GGYRCC 1 cut(s) 3438
BarI GAAGNNNNNNTAC 2 cut(s) 4152, 4184
BauI CACGAG 2 cut(s) 4972, 6435
BbrPI CACGTG 1 cut(s) 5129
BbsI GAAGAC 3 cut(s) 1554, 2631, 6472
Bbv12I GWGCWC 6 cut(s) 274, 310, 722, 4708, 5647, 7585
BceAI ACGGC 3 cut(s) 1777, 6874, 7809
BcgI CGANNNNNNTGC 4 cut(s) 2925, 2959, 6941, 6975
BciT130I CCWGG 9 cut(s) 435, 900, 2075, 2402, 3017, 4891, 4902, 7125, 7229
BciVI GTATCC 2 cut(s) 258, 7444
BclI TGATCA 1 cut(s) 6921
BcnI CCSGG 1 cut(s) 6874
BcuI ACTAGT 1 cut(s) 3467
BfmI CTRYAG 7 cut(s) 1914, 2985, 3996, 5316, 6725, 7590, 7695
BfuAI ACCTGC 4 cut(s) 1934, 4669, 5180, 5849
BfuI GTATCC 2 cut(s) 258, 7444
BglI GCCNNNNNGGC 1 cut(s) 5975
BglII AGATCT 1 cut(s) 4240
BlnI CCTAGG 1 cut(s) 6701
BlpI GCTNAGC 2 cut(s) 6963, 7488
BmcAI AGTACT 1 cut(s) 559
Bme18I GGWCC 9 cut(s) 147, 277, 893, 1169, 2138, 2581, 3019, 3328, 4690
BmeRI GACNNNNNGTC 2 cut(s) 1290, 7117
BmeT110I CYCGRG 2 cut(s) 2944, 6929
BmgBI CACGTC 1 cut(s) 7037
BmiI GGNNCC 6 cut(s) 149, 894, 1278, 1791, 2399, 3440
BmrI ACTGGG 4 cut(s) 3399, 4659, 5212, 5543
BmuI ACTGGG 4 cut(s) 3399, 4659, 5212, 5543
BoxI GACNNNNGTC 1 cut(s) 2777
BpiI GAAGAC 3 cut(s) 1554, 2631, 6472
BpmI CTGGAG 3 cut(s) 1204, 2851, 6324
Bpu10I CCTNAGC 4 cut(s) 1165, 3549, 5475, 6379
Bpu1102I GCTNAGC 2 cut(s) 6963, 7488
BpuEI CTTGAG 9 cut(s) 1311, 2298, 2483, 3098, 4478, 4796, 7161, 7583, 7688
BpuMI CCSGG 1 cut(s) 6874
Bsa29I ATCGAT 1 cut(s) 7270
BsaAI YACGTR 3 cut(s) 5129, 5899, 7054
BsaBI GATNNNNATC 2 cut(s) 6159, 6408
BsaHI GRCGYC 1 cut(s) 2774
BsaI GGTCTC 2 cut(s) 2827, 6499
BsaJI CCNNGG 9 cut(s) 406, 2401, 2640, 4889, 4890, 5421, 5848, 6701, 7227
BsaWI WCCGGW 2 cut(s) 4310, 4591
BsaXI ACNNNNNCTCC 4 cut(s) 1446, 1476, 5108, 5138
Bse21I CCTNAGG 4 cut(s) 1288, 5112, 7520, 7547
Bse3DI GCAATG 5 cut(s) 1189, 1791, 2602, 2931, 6924
Bse8I GATNNNNATC 2 cut(s) 6159, 6408
BseBI CCWGG 9 cut(s) 435, 900, 2075, 2402, 3017, 4891, 4902, 7125, 7229
BseCI ATCGAT 1 cut(s) 7270
BseDI CCNNGG 9 cut(s) 406, 2401, 2640, 4889, 4890, 5421, 5848, 6701, 7227
BseJI GATNNNNATC 2 cut(s) 6159, 6408
BseMI GCAATG 5 cut(s) 1189, 1791, 2602, 2931, 6924
BseRI GAGGAG 6 cut(s) 1352, 3524, 5129, 6236, 6419, 7489
BseSI GKGCMC 4 cut(s) 722, 979, 5647, 7585
BsgI GTGCAG 3 cut(s) 1806, 4305, 4692
Bsh1236I CGCG 1 cut(s) 5423
BshNI GGYRCC 1 cut(s) 3438
BshVI ATCGAT 1 cut(s) 7270
BsiHKAI GWGCWC 6 cut(s) 274, 310, 722, 4708, 5647, 7585
BsiHKCI CYCGRG 2 cut(s) 2944, 6929
BsiSI CCGG 3 cut(s) 4311, 4592, 6874
BslFI GGGAC 6 cut(s) 133, 2981, 2987, 5738, 6628, 6946
BsmBI CGTCTC 2 cut(s) 179, 1148
BsmFI GGGAC 6 cut(s) 133, 2981, 2987, 5738, 6628, 6946
BsmI GAATGC 6 cut(s) 31, 1637, 5622, 6350, 6847, 7690
Bso31I GGTCTC 2 cut(s) 2827, 6499
BsoBI CYCGRG 2 cut(s) 2944, 6929
Bsp1286I GDGCHC 7 cut(s) 274, 310, 722, 979, 4708, 5647, 7585
Bsp1407I TGTACA 3 cut(s) 2921, 4036, 7652
Bsp1720I GCTNAGC 2 cut(s) 6963, 7488
BspDI ATCGAT 1 cut(s) 7270
BspFNI CGCG 1 cut(s) 5423
BspHI TCATGA 7 cut(s) 736, 1617, 3694, 5146, 6019, 6400, 7537
BspLI GGNNCC 6 cut(s) 149, 894, 1278, 1791, 2399, 3440
BspMAI CTGCAG 2 cut(s) 7594, 7699
BspMI ACCTGC 4 cut(s) 1934, 4669, 5180, 5849
BspPI GGATC 8 cut(s) 2065, 2436, 2640, 3147, 3315, 4045, 4212, 5670
BspQI GCTCTTC 3 cut(s) 1916, 2878, 5001
BspT107I GGYRCC 1 cut(s) 3438
BspTNI GGTCTC 2 cut(s) 2827, 6499
BsrDI GCAATG 5 cut(s) 1189, 1791, 2602, 2931, 6924
BsrGI TGTACA 3 cut(s) 2921, 4036, 7652
BssECI CCNNGG 9 cut(s) 406, 2401, 2640, 4889, 4890, 5421, 5848, 6701, 7227
BssNAI GTATAC 2 cut(s) 2705, 6274
BssNI GRCGYC 1 cut(s) 2774
BssSI CACGAG 2 cut(s) 4972, 6435
BssT1I CCWWGG 3 cut(s) 2640, 5848, 6701
Bst1107I GTATAC 2 cut(s) 2705, 6274
Bst2BI CACGAG 2 cut(s) 4972, 6435
Bst2UI CCWGG 9 cut(s) 435, 900, 2075, 2402, 3017, 4891, 4902, 7125, 7229
BstACI GRCGYC 1 cut(s) 2774
BstAPI GCANNNNNTGC 6 cut(s) 314, 1949, 5243, 5333, 7589, 7694
BstAUI TGTACA 3 cut(s) 2921, 4036, 7652
BstBAI YACGTR 3 cut(s) 5129, 5899, 7054
BstC8I GCNNGC 8 cut(s) 841, 1878, 2820, 3107, 4740, 5104, 5227, 5939
BstDSI CCRYGG 2 cut(s) 406, 5421
BstENI CCTNNNNNAGG 1 cut(s) 5852
BstFNI CGCG 1 cut(s) 5423
BstNI CCWGG 9 cut(s) 435, 900, 2075, 2402, 3017, 4891, 4902, 7125, 7229
BstPAI GACNNNNGTC 1 cut(s) 2777
BstSFI CTRYAG 7 cut(s) 1914, 2985, 3996, 5316, 6725, 7590, 7695
BstSLI GKGCMC 4 cut(s) 722, 979, 5647, 7585
BstSNI TACGTA 1 cut(s) 5899
BstUI CGCG 1 cut(s) 5423
BstV2I GAAGAC 3 cut(s) 1554, 2631, 6472
BstX2I RGATCY 6 cut(s) 2632, 3139, 3307, 4050, 4240, 5675
BstXI CCANNNNNNTGG 2 cut(s) 124, 3105
BstYI RGATCY 6 cut(s) 2632, 3139, 3307, 4050, 4240, 5675
BstZ17I GTATAC 2 cut(s) 2705, 6274
Bsu15I ATCGAT 1 cut(s) 7270
Bsu36I CCTNAGG 4 cut(s) 1288, 5112, 7520, 7547
BsuI GTATCC 2 cut(s) 258, 7444
BsuTUI ATCGAT 1 cut(s) 7270
BtgI CCRYGG 2 cut(s) 406, 5421
BtgZI GCGATG 2 cut(s) 5791, 6340
BtrI CACGTC 1 cut(s) 7037
BtsI GCAGTG 5 cut(s) 1749, 3456, 4317, 5580, 6675
BveI ACCTGC 4 cut(s) 1934, 4669, 5180, 5849
Cac8I GCNNGC 8 cut(s) 841, 1878, 2820, 3107, 4740, 5104, 5227, 5939
CaiI CAGNNNCTG 5 cut(s) 830, 2829, 3389, 3506, 5333
CciI TCATGA 7 cut(s) 736, 1617, 3694, 5146, 6019, 6400, 7537
Cfr42I CCGCGG 1 cut(s) 5424
ClaI ATCGAT 1 cut(s) 7270
CsiI ACCWGGT 1 cut(s) 7123
CspCI CAANNNNNGTGG 2 cut(s) 3099, 3134
DraI TTTAAA 3 cut(s) 574, 4270, 6837
DraIII CACNNNGTG 2 cut(s) 220, 5175
DriI GACNNNNNGTC 2 cut(s) 1290, 7117
EaeI YGGCCR 4 cut(s) 3107, 3905, 5166, 7794
Eam1105I GACNNNNNGTC 2 cut(s) 1290, 7117
Eco105I TACGTA 1 cut(s) 5899
Eco130I CCWWGG 3 cut(s) 2640, 5848, 6701
Eco31I GGTCTC 2 cut(s) 2827, 6499
Eco32I GATATC 4 cut(s) 370, 3679, 4719, 7224
Eco47I GGWCC 9 cut(s) 147, 277, 893, 1169, 2138, 2581, 3019, 3328, 4690
Eco72I CACGTG 1 cut(s) 5129
Eco81I CCTNAGG 4 cut(s) 1288, 5112, 7520, 7547
Eco88I CYCGRG 2 cut(s) 2944, 6929
EcoNI CCTNNNNNAGG 1 cut(s) 5852
EcoO109I RGGNCCY 4 cut(s) 893, 4771, 5968, 7002
EcoRI GAATTC 4 cut(s) 3208, 3829, 6094, 6613
EcoRII CCWGG 9 cut(s) 433, 898, 2073, 2400, 3015, 4889, 4900, 7123, 7227
EcoRV GATATC 4 cut(s) 370, 3679, 4719, 7224
EcoT14I CCWWGG 3 cut(s) 2640, 5848, 6701
EcoT22I ATGCAT 3 cut(s) 1393, 2025, 4188
ErhI CCWWGG 3 cut(s) 2640, 5848, 6701
Esp3I CGTCTC 2 cut(s) 179, 1148
FalI AAGNNNNNCTT 2 cut(s) 7278, 7310
FaqI GGGAC 6 cut(s) 133, 2981, 2987, 5738, 6628, 6946
FauI CCCGC 2 cut(s) 5715, 7737
FauNDI CATATG 7 cut(s) 349, 421, 1580, 1591, 2305, 4209, 4275
FbaI TGATCA 1 cut(s) 6921
FblI GTMKAC 2 cut(s) 2704, 6273
GsuI CTGGAG 3 cut(s) 1204, 2851, 6324
HapII CCGG 3 cut(s) 4311, 4592, 6874
Hin1I GRCGYC 1 cut(s) 2774
HincII GTYRAC 5 cut(s) 1029, 1263, 2392, 7093, 7513
HindII GTYRAC 5 cut(s) 1029, 1263, 2392, 7093, 7513
HindIII AAGCTT 5 cut(s) 672, 2816, 5697, 6581, 6965
HpaI GTTAAC 1 cut(s) 1263
HpaII CCGG 3 cut(s) 4311, 4592, 6874
HpyCH4IV ACGT 7 cut(s) 172, 2194, 2774, 5128, 5898, 7036, 7053
HpySE526I ACGT 7 cut(s) 172, 2194, 2774, 5128, 5898, 7036, 7053
Hsp92I GRCGYC 1 cut(s) 2774
Ksp22I TGATCA 1 cut(s) 6921
KspAI GTTAAC 1 cut(s) 1263
KspI CCGCGG 1 cut(s) 5424
LguI GCTCTTC 3 cut(s) 1916, 2878, 5001
LmnI GCTCC 8 cut(s) 223, 1093, 1789, 2086, 3992, 4594, 6432, 7476
MabI ACCWGGT 1 cut(s) 7123
MaeII ACGT 7 cut(s) 172, 2194, 2774, 5128, 5898, 7036, 7053
MfeI CAATTG 4 cut(s) 921, 1826, 4329, 6089
MflI RGATCY 6 cut(s) 2632, 3139, 3307, 4050, 4240, 5675
MhlI GDGCHC 7 cut(s) 274, 310, 722, 979, 4708, 5647, 7585
MlsI TGGCCA 2 cut(s) 3109, 5168
MluNI TGGCCA 2 cut(s) 3109, 5168
MmeI TCCRAC 6 cut(s) 3355, 4374, 4642, 5721, 5748, 6541
Mox20I TGGCCA 2 cut(s) 3109, 5168
Mph1103I ATGCAT 3 cut(s) 1393, 2025, 4188
MroXI GAANNNNTTC 7 cut(s) 198, 210, 1074, 2544, 3613, 4452, 7152
MscI TGGCCA 2 cut(s) 3109, 5168
MslI CAYNNNNRTG 5 cut(s) 861, 1475, 5585, 5655, 6312
Msp20I TGGCCA 2 cut(s) 3109, 5168
MspA1I CMGCKG 5 cut(s) 2455, 3842, 5225, 5423, 6524
MspI CCGG 3 cut(s) 4311, 4592, 6874
MssI GTTTAAAC 1 cut(s) 4270
MunI CAATTG 4 cut(s) 921, 1826, 4329, 6089
Mva1269I GAATGC 6 cut(s) 31, 1637, 5622, 6350, 6847, 7690
MvaI CCWGG 9 cut(s) 435, 900, 2075, 2402, 3017, 4891, 4902, 7125, 7229
MvnI CGCG 1 cut(s) 5423
NciI CCSGG 1 cut(s) 6874
NdeI CATATG 7 cut(s) 349, 421, 1580, 1591, 2305, 4209, 4275
NlaIV GGNNCC 6 cut(s) 149, 894, 1278, 1791, 2399, 3440
NsiI ATGCAT 3 cut(s) 1393, 2025, 4188
PaeI GCATGC 2 cut(s) 1880, 4742
PaeR7I CTCGAG 1 cut(s) 2944
PagI TCATGA 7 cut(s) 736, 1617, 3694, 5146, 6019, 6400, 7537
PaqCI CACCTGC 1 cut(s) 5180
PasI CCCWGGG 1 cut(s) 4890
PciI ACATGT 5 cut(s) 681, 3182, 3574, 6768, 7116
PciSI GCTCTTC 3 cut(s) 1916, 2878, 5001
PcsI WCGNNNNNNNCGW 1 cut(s) 3019
PctI GAATGC 6 cut(s) 31, 1637, 5622, 6350, 6847, 7690
PdmI GAANNNNTTC 7 cut(s) 198, 210, 1074, 2544, 3613, 4452, 7152
PflFI GACNNNGTC 1 cut(s) 2196
PflMI CCANNNNNTGG 3 cut(s) 1178, 2829, 3337
PfoI TCCNGGA 1 cut(s) 2073
PmaCI CACGTG 1 cut(s) 5129
PmeI GTTTAAAC 1 cut(s) 4270
PmlI CACGTG 1 cut(s) 5129
Ppu21I YACGTR 3 cut(s) 5129, 5899, 7054
PpuMI RGGWCCY 1 cut(s) 893
PscI ACATGT 5 cut(s) 681, 3182, 3574, 6768, 7116
PshAI GACNNNNGTC 1 cut(s) 2777
PshBI ATTAAT 3 cut(s) 2490, 3054, 7308
PsiI TTATAA 1 cut(s) 3860
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 9 cut(s) 433, 898, 2073, 2400, 3015, 4889, 4900, 7123, 7227
PspCI CACGTG 1 cut(s) 5129
PspGI CCWGG 9 cut(s) 433, 898, 2073, 2400, 3015, 4889, 4900, 7123, 7227
PspN4I GGNNCC 6 cut(s) 149, 894, 1278, 1791, 2399, 3440
PspPPI RGGWCCY 1 cut(s) 893
PsrI GAACNNNNNNTAC 4 cut(s) 2790, 2822, 4854, 4886
PstI CTGCAG 2 cut(s) 7594, 7699
PstNI CAGNNNCTG 5 cut(s) 830, 2829, 3389, 3506, 5333
PsuI RGATCY 6 cut(s) 2632, 3139, 3307, 4050, 4240, 5675
PsyI GACNNNGTC 1 cut(s) 2196
PvuII CAGCTG 3 cut(s) 3842, 5225, 6524
RseI CAYNNNNRTG 5 cut(s) 861, 1475, 5585, 5655, 6312
SacII CCGCGG 1 cut(s) 5424
SapI GCTCTTC 3 cut(s) 1916, 2878, 5001
ScaI AGTACT 1 cut(s) 559
SduI GDGCHC 7 cut(s) 274, 310, 722, 979, 4708, 5647, 7585
SexAI ACCWGGT 1 cut(s) 7123
SfcI CTRYAG 7 cut(s) 1914, 2985, 3996, 5316, 6725, 7590, 7695
Sfr274I CTCGAG 1 cut(s) 2944
Sfr303I CCGCGG 1 cut(s) 5424
SgrBI CCGCGG 1 cut(s) 5424
SinI GGWCC 9 cut(s) 147, 277, 893, 1169, 2138, 2581, 3019, 3328, 4690
SlaI CTCGAG 1 cut(s) 2944
SmiMI CAYNNNNRTG 5 cut(s) 861, 1475, 5585, 5655, 6312
SnaBI TACGTA 1 cut(s) 5899
SpeI ACTAGT 1 cut(s) 3467
SphI GCATGC 2 cut(s) 1880, 4742
SspI AATATT 3 cut(s) 5307, 5892, 6536
StyI CCWWGG 3 cut(s) 2640, 5848, 6701
TaiI ACGT 7 cut(s) 175, 2197, 2777, 5131, 5901, 7039, 7056
TatI WGTACW 5 cut(s) 557, 2921, 4036, 5544, 7652
TauI GCSGC 3 cut(s) 3910, 5423, 7245
TspGWI ACGGA 4 cut(s) 2771, 3341, 3841, 7064
Tth111I GACNNNGTC 1 cut(s) 2196
Van91I CCANNNNNTGG 3 cut(s) 1178, 2829, 3337
VneI GTGCAC 3 cut(s) 718, 5643, 7581
VpaK11BI GGWCC 9 cut(s) 147, 277, 893, 1169, 2138, 2581, 3019, 3328, 4690
VspI ATTAAT 3 cut(s) 2490, 3054, 7308
XagI CCTNNNNNAGG 1 cut(s) 5852
XbaI TCTAGA 2 cut(s) 1132, 2051
XcmI CCANNNNNNNNNTGG 4 cut(s) 1179, 2647, 3980, 6265
XhoI CTCGAG 1 cut(s) 2944
XmaJI CCTAGG 1 cut(s) 6701
XmiI GTMKAC 2 cut(s) 2704, 6273
XmnI GAANNNNTTC 7 cut(s) 198, 210, 1074, 2544, 3613, 4452, 7152
ZraI GACGTC 1 cut(s) 2775
ZrmI AGTACT 1 cut(s) 559
Zsp2I ATGCAT 3 cut(s) 1393, 2025, 4188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.