FvH4_4g36210

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000223
Physical Location & Seq
Forward (+)
33240425 .. 33250175
9751 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36210.t1

Sequence Viewer

Length: 5001 bp
ATGACTCAAAACATTCCTCGATGGGTTCGTTCCGTTCGCGAGGAGACAGAACCTGTCAGCTCTGGCAATGAGGCATCAGAGGAGAAACTTCAGGCGCTTCTTAATATTGCAATGGCTTGTGTTTCGATTACACCAGAGAACAGACCTACAATGAGAAAGGTTTTGAGCATGATAAGGGATTCGAGGGCAGATGCTATACTGTCTTTGAACAGCAGCAGTGATCATTCTCCGGGGAAATGGGAGTGCCTTATGTGTGGGAATGCCATTGGAGAAAAGAAGGGAGGTGTTTCTTGTCAGGCAACTGCAACTCTCCTTGGAAAAAGTTCATTGAGGATCTATGTGTCATGTGACAATATTCTGTCTGAGCTTCCAGAAGGTTATAACAGTGAGCAGCCTAAAAATACAACAGTTCTAGAATGTGCTGTGGTGTCTGACACTGTTGTAGAACAAAGAGAGTCAGACTTTTCTGAAGGCTCTTTAATTGGAAATATCAGTCCAGAGTTTCTGATTGATGTGGTTCGGGAACTTCTTTTTGATAAGATAAATATGATTAAATTGATTAAAAAGTGTACCAAGAGTTGGAAGATGCTGGAAGATGTATTACTTGCTACTGATGCAGAGATGGGGTTTTGTGTTTTGTTTGCTTTATCTGAATTTGGTATGGGTTTTGATATTCTTTTGGATATGCTGAGGAGGAAGTACATTTCTGAACAGCTACCAAAGGAGTTTTGTTATGTCCTTAAGGGAATTGCATCTTTACGTGATTCTCTGGTGGTTACAAGTTTCGGGGTTGAATGTTCAAGGTTCTGCTGTTCAGATGTCATTTGCATCTACATGAAGGCTAACCTATGCACTGGTGAAATTCTGAAATCAAAACATCCTGTGGCTCTTAAAGGGTTTCAAACCAACAAAGCTAAGGGAAGAGAACAGTGCAATAGATTTTCAGATGCCTCAACAATCAAGTTGGCTTTGGAGAAGATTATTTGGCTTTTCGCTGCTGCATGGTCTGGTGTTGGCAAAGAAGAACATAAGTTATCTAGACAAGTGCTGAAAATGCTGGATGAATTGGTGCAATTCTACGCCACATTAGATGGAAGGTCAGTTATAAACATTTCGATTGTCTTGACAAGGAAAGCAAGCGCCTTTGAGTTGGAAAAGGTGAAGATGGAAGCTTCACACAAGAAGAAAGGTAAAAGACCCAACCTTTTGGCCGATACAGTCTTCTCTTGGTCCATTGATGACGTTCTCAACGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAGTCATTTCAGTCTGTTGAGCATTACTTCGGGTGTTATGTGTATCCTTTATTAGAAGAAACACGAGCACAAGTCCATTCAAGTATGGAGACTATTCACAGGGCACCATATGCTAAAGTAGTTTCTTTTGAAAATGCCAAGCCATATGGAAGAAAGCTCTACAATGTCAAGGTTGATTACTGGCGAAACAGGTTCAATGACCGTGGCAAGGAGCCATACAAAACTTTGCCTGGTGATCTCTTTGTTTTAGCAAATGCTAAACTTGAAACTGTTTCTGATTTACAAAGGTTAGGGAGGTCGTGGGCTTTTGGATCAGTCACTAAAGTGTCAGAAAATGAGAACAAGGATGACACTACTTCTCTTTATTTTAAAGTCAAGGCTTCCAAAGAGCTTAAAGTCTTAAAGAGCACAACACCATTGTTTATGGTTTTCCTAGTGAACTTAATACCAAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACTTGAAGATTGTCAAGGAGGTTCTGTGCACTGATAATGTGGCTCAGAAAAGTGTCTGCTCTGAAAAGAACAGTGACATTGTGAATATGGGATTGGTACAGAGTTTATCATCTGGCTTGAGTGAATCCCAAACTGGGACTGTTTTAGCCTGTCTTGAAATGCTGCATTCTCGTGACAAGTCTGCTGTGGAACTGATTTGGGGTCCTCCTGGTACTGGAAAGACGAAAACCATTGTCACTCTACTCTTAAGCCTGCTACAGATGAATTGCAGGACTCTTGTTTGTGCCCCAACAAATGTTGCAATAACTGAAATTGCTTCTCGCCTTGTAAAGATGGTGACTGAAGTAGAGTCCAATGCTCCGTATTGTTCTTTGGGAAAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAAAATTGGCTCAGACATTGAAGAGATATATTTAGATTTCCGTCTCAAAAGGCTTGTTGAGTGCCTAGGGCCACAGACTGGTTGGAGGCATTGCTTTACTTCCATGATAGAATGTCTTGAAGATGGTGTTTCTCACTACCGTACTTTTCTGGGAAACGGGTTAACTATTGAGAAGGAACTGAAGACTACAGGCCAAATGCAAGTGAAAGAAAGTAGAATTGTCACTAAAGTTGGCAAGGACAAGTGCAAAACATTTCTGGAGTTTATAAGAGATAGATTTGTTTCTATTGCATCACCTCTAAGATATTGCATTTCTATCTTCTGTACCCACATGGCGAAAAATCACATTTCCGCAGATATTTTCCAAAGCATGGTTTCGCTTGTCAACCTGGTAGATTCCTTTGAATCATTGTTGTTTCAAGGTAATGTTGCTCCTGAAGCACTTGAAGACATTTTCTCTCGTTCTGATGTAGAAGACATATCAGAGACATGTGTTGATAACTCTTTTCTTCTATTCACAAATAGAAGAGAATGCCTTAAAGTTTTGCATAAACTATTTGACTCTCTTAGAAAACTTGATCTTCCGGACTTTATGAAGCAAGACTCATATAAGTTGCATAAACTGGCAATGGAGCCATTAAGCATTGTGGTTATTGACGAAGCTGCACAATTGAAAGAGTGTGAATCAACAATCCCCCTTCAACTACCAGGTGTGAGGCATGCTGTTCTTGTTGGTGACGAATGTCAGTTACCTGCTATAGTAAAGAGCACTGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCTTAGTTGGTCACTCAAAACACCTCCTGAATATGCAGTACAGGATGCATCCATCAATCAGTTCCTTCCCAAATTCTAGTTTCTACAATAACATGATCCTGGATGCTCCATATATCAAAAGGAGAGGCCAGGAGGAAAGTTATCTTAAAGGGTCAATGTTTGGTCCTTACTCTTTTATCAATGTAATTGGTGGACAAGAAGAGAAAGACGAGGATGGATGTAGTCGGAAGAATATGGTTGAGAATCTCAGTATTGGTGTAGTTTCTTCATATGCTGCTCAAGTAGTTGCAATCGAGGACAAACTTGGAAAGAAATATAATAATCTTGATGGATTTATAGTGAAAGTGAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTTTCCACTGTACGATCCAATTGTCAACAGTCACTTGAGTTTATTTCAAAACCGCAGATAATTAATGTTGCTCTTACAAGGGCAAGGCACTGCCTGTGGATTTTGGGGAATGAAAGAACGCTATGTGAAAGTGAGTCTGTTTGGGAGGCCTTGGTCCTTGATGCCAAAAATCGCCAATGCTTTTTTAATGCTGATGAAGACGAGGATTTAGCCAAGGCCATACTACAAGTGAAGAAACAGTTCGACCAACTAGATGATTTGCTTAATTCTGACAGTGTACTTTTCAGAAGAGCTAGATGGAAGGTACTTTTTAGCGATGATTTTTTGAATTCATTCAAGAAACTGAAGTCAGTCAGTTTGAAGAGGTCAGTGATAAATGTTCTACTAAAGCTTGCCAGTGGCTGGAGACCGAAGATGCAAAGTTCAGATATACTTTGTGGTAACTCTTCACTGATCCTGAAGAAATTTAAGGTTGAAGACCTATATATTGTTTGCACAACAGACATAGCAAAGGATTTGAGATATATACAAGTATTGAAGATTTGGGACATAATGCCTATAGAGGATATCCCAGAGTTGGTAAATCGCATGGACAATATGTTAAACAAATATACAGATGAGTATATTAATCTGTGTAAGGAGAAATGTCTCGAGGGTGATCTGGAAGTTCCAAAGAGCTGGCCAAGTTTGAAGGACCCGGAGTACCTTAGTCTGCATGAGAGCAACACTGAGGCTGAGCTTACTCAGTATTTCATGGGATCATGTCACGGGACCACAGTTTTTTGGATACGCTGTTCGCCAACAGAGCTACTAGTTGGTGTTGATTCACTAAAGAGTCAAACATATAAAGCGGTCGATCCAAAACATAAGAGAACTGGTTATGAGCTTCTGCTGAGCTTTAATGATCAGAAGTTCATAGAGATCGTTCCAAATGTTTATGCCACTCTATGTGGAGATGCCATCAGGGCTGCCATTGAGTACTTTCATGAGTCTCCATTGGACCCTGCACATGATGTTAGGCTTGTGGGGGATGGGCTTGATCAGGCGATCAACGTAAAAGATAATGTTTTTGGATCTGGAAGTGGAAGCTTGCTCGGATTATGTCAGTTAAGAAAGCCAGAATCAGCAAGGGACATGGATGCCTTAGTCAAAGAATTAATGGAAGCGGCAAAGAATGATCCTTACACTGGTGGAGTTATTCAAGGATCTGTGTTCGATGAGTTGTATCGTCATAAAATTGAGTATGAAAACAAGTTTTATGATGGAAAAACCATGATTTTCATCATTCAAAACACAGATGAACGCATCTTGAGTCCATTGGATTTGGAATTACATCTACGAAGCTTTGACACAGGTCTACGTAGGAAAAGTATACTTTGGTTATGTTCTCCAACAGGCTTTCTGGTTTACCTTGTCAAACTGTCCTCACCAGATTGTTGCAAGAATTGGTTCGGTTCGTGTAATGTTTTTGGAAACCATATTCGGCAGCAAACTTATACCAACACAGACGGTGAAATTTGTGATTATTATCTAGCGTATGCTAGCAGGGAGGTATTTGATAGAGCTGCTAGGATGACATCGTTTACGGACTTGGTTAAGGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1667

Amino Acids

188.28

Weight (kDa)

6.47

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 465 - 593 1e-60 Domain of unknown function (DUF6469)
AAA_11 PF13086 633 - 749 2.6e-15 AAA domain
AAA_19 PF13245 651 - 981 3.9e-08 AAA domain
AAA_11 PF13086 932 - 984 1.3e-11 AAA domain
AAA_12 PF13087 993 - 1081 1.5e-15 AAA domain
AAA_12 PF13087 1079 - 1169 1.2e-26 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 381, 1106, 2450
Acc36I ACCTGC 1 cut(s) 2944
AccB1I GGYRCC 1 cut(s) 1390
AccB7I CCANNNNNTGG 4 cut(s) 579, 2261, 2554, 3795
AccI GTMKAC 2 cut(s) 4750, 4765
AccII CGCG 1 cut(s) 39
AccIII TCCGGA 1 cut(s) 2767
AciI CCGC 4 cut(s) 2535, 3446, 4244, 4559
AcoI YGGCCR 2 cut(s) 1209, 4073
AcsI RAATTY 6 cut(s) 653, 861, 3073, 3721, 3857, 4908
AcuI CTGAAG 7 cut(s) 74, 489, 2136, 2384, 2640, 3758, 3872
AflII CTTAAG 2 cut(s) 740, 2020
AflIII ACRYGT 2 cut(s) 1764, 2672
AhlI ACTAGT 1 cut(s) 4204
AjnI CCWGG 7 cut(s) 1516, 1981, 2571, 2890, 3099, 3129, 3366
AjuI GAANNNNNNNTTGG 2 cut(s) 1850, 1882
AleI CACNNNNGTG 1 cut(s) 1610
AloI GAACNNNNNNTCC 2 cut(s) 4171, 4203
Alw21I GWGCWC 4 cut(s) 1357, 1697, 1805, 2954
Alw26I GTCTC 8 cut(s) 38, 1262, 1370, 2231, 2663, 3793, 4046, 4389
Alw44I GTGCAC 1 cut(s) 1801
AlwNI CAGNNNCTG 2 cut(s) 53, 3795
Ama87I CYCGRG 1 cut(s) 4043
Aor13HI TCCGGA 1 cut(s) 2767
AoxI GGCC 7 cut(s) 1209, 2252, 2374, 3127, 3540, 3609, 4073
ApaLI GTGCAC 1 cut(s) 1801
ApoI RAATTY 6 cut(s) 653, 861, 3073, 3721, 3857, 4908
AseI ATTAAT 3 cut(s) 3456, 4020, 4550
Asp700I GAANNNNTTC 5 cut(s) 322, 1281, 3632, 3725, 4841
AspA2I CCTAGG 1 cut(s) 2248
AspLEI GCGC 2 cut(s) 97, 1142
AspS9I GGNCC 8 cut(s) 1230, 1976, 2252, 3164, 3547, 4087, 4164, 4393
AsuC2I CCSGG 2 cut(s) 231, 4091
AsuHPI GGTGA 9 cut(s) 869, 1171, 1532, 2122, 2469, 2930, 4061, 4812, 4916
AsuNHI GCTAGC 1 cut(s) 4934
AvaI CYCGRG 1 cut(s) 4043
AvaII GGWCC 7 cut(s) 1230, 1976, 3164, 3547, 4087, 4164, 4393
AvrII CCTAGG 1 cut(s) 2248
BaeGI GKGCMC 3 cut(s) 1393, 1805, 2062
BalI TGGCCA 1 cut(s) 4075
BanI GGYRCC 1 cut(s) 1390
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BauI CACGAG 2 cut(s) 1350, 1944
BbsI GAAGAC 7 cut(s) 1213, 2372, 2637, 2664, 3356, 3597, 3876
Bbv12I GWGCWC 4 cut(s) 1357, 1697, 1805, 2954
BbvCI CCTCAGC 1 cut(s) 689
BciT130I CCWGG 7 cut(s) 1518, 1983, 2573, 2892, 3101, 3131, 3368
BciVI GTATCC 2 cut(s) 1341, 4173
BclI TGATCA 3 cut(s) 220, 4297, 4432
BcnI CCSGG 2 cut(s) 231, 4091
BcoDI GTCTC 8 cut(s) 38, 1262, 1370, 2231, 2663, 3793, 4046, 4389
BcuI ACTAGT 1 cut(s) 4204
BfmI CTRYAG 4 cut(s) 2030, 2370, 2940, 3951
BfoI RGCGCY 2 cut(s) 98, 1143
BfrI CTTAAG 2 cut(s) 740, 2020
BfuAI ACCTGC 1 cut(s) 2944
BfuI GTATCC 2 cut(s) 1341, 4173
BglI GCCNNNNNGGC 1 cut(s) 4358
BlnI CCTAGG 1 cut(s) 2248
BlpI GCTNAGC 2 cut(s) 4128, 4286
BmcAI AGTACT 1 cut(s) 4373
Bme1390I CCNGG 9 cut(s) 231, 1518, 1983, 2573, 2892, 3101, 3131, 3368, 4091
Bme18I GGWCC 7 cut(s) 1230, 1976, 3164, 3547, 4087, 4164, 4393
BmeT110I CYCGRG 1 cut(s) 4043
BmgT120I GGNCC 8 cut(s) 1230, 1976, 2252, 3164, 3547, 4087, 4164, 4393
BmiI GGNNCC 8 cut(s) 1392, 1500, 1977, 2817, 3365, 4089, 4165, 4395
BmrFI CCNGG 9 cut(s) 231, 1518, 1983, 2573, 2892, 3101, 3131, 3368, 4091
BmrI ACTGGG 1 cut(s) 1917
BmtI GCTAGC 1 cut(s) 4938
BmuI ACTGGG 1 cut(s) 1917
BoxI GACNNNNGTC 2 cut(s) 2925, 4746
BpiI GAAGAC 7 cut(s) 1213, 2372, 2637, 2664, 3356, 3597, 3876
BpmI CTGGAG 2 cut(s) 2462, 3817
Bpu10I CCTNAGC 2 cut(s) 689, 915
Bpu1102I GCTNAGC 2 cut(s) 4128, 4286
BpuEI CTTGAG 4 cut(s) 1912, 3264, 3449, 4723
BpuMI CCSGG 2 cut(s) 231, 4091
BsaAI YACGTR 2 cut(s) 761, 4754
BsaBI GATNNNNATC 2 cut(s) 4673, 4920
BsaI GGTCTC 1 cut(s) 3793
BsaJI CCNNGG 7 cut(s) 230, 313, 1489, 2248, 3367, 3543, 3606
BsaWI WCCGGW 1 cut(s) 2767
BsaXI ACNNNNNCTCC 2 cut(s) 1784, 1814
Bse1I ACTGG 9 cut(s) 859, 1472, 1912, 1993, 2266, 2811, 3789, 4273, 4585
Bse3DI GCAATG 4 cut(s) 73, 117, 2272, 2817
Bse8I GATNNNNATC 2 cut(s) 4673, 4920
BseAI TCCGGA 1 cut(s) 2767
BseBI CCWGG 7 cut(s) 1518, 1983, 2573, 2892, 3101, 3131, 3368
BseDI CCNNGG 7 cut(s) 230, 313, 1489, 2248, 3367, 3543, 3606
BseJI GATNNNNATC 2 cut(s) 4673, 4920
BseMI GCAATG 4 cut(s) 73, 117, 2272, 2817
BseNI ACTGG 9 cut(s) 859, 1472, 1912, 1993, 2266, 2811, 3789, 4273, 4585
BseRI GAGGAG 3 cut(s) 56, 95, 706
BseSI GKGCMC 3 cut(s) 1393, 1805, 2062
BsgI GTGCAG 2 cut(s) 2832, 4383
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 4248
BshFI GGCC 7 cut(s) 1211, 2254, 2376, 3129, 3542, 3611, 4075
BshNI GGYRCC 1 cut(s) 1390
BsiEI CGRYCG 1 cut(s) 4248
BsiHKAI GWGCWC 4 cut(s) 1357, 1697, 1805, 2954
BsiHKCI CYCGRG 1 cut(s) 4043
BsiSI CCGG 3 cut(s) 230, 2768, 4091
BslFI GGGAC 4 cut(s) 1924, 3953, 4177, 4538
BsmAI GTCTC 8 cut(s) 38, 1262, 1370, 2231, 2663, 3793, 4046, 4389
BsmBI CGTCTC 2 cut(s) 1262, 2231
BsmFI GGGAC 4 cut(s) 1924, 3953, 4177, 4538
BsmI GAATGC 3 cut(s) 265, 1939, 2720
BsnI GGCC 7 cut(s) 1211, 2254, 2376, 3129, 3542, 3611, 4075
Bso31I GGTCTC 1 cut(s) 3793
BsoBI CYCGRG 1 cut(s) 4043
Bsp1286I GDGCHC 6 cut(s) 1357, 1393, 1697, 1805, 2062, 2954
Bsp13I TCCGGA 1 cut(s) 2767
Bsp1720I GCTNAGC 2 cut(s) 4128, 4286
Bsp68I TCGCGA 1 cut(s) 39
BspACI CCGC 4 cut(s) 2535, 3446, 4244, 4559
BspANI GGCC 7 cut(s) 1211, 2254, 2376, 3129, 3542, 3611, 4075
BspEI TCCGGA 1 cut(s) 2767
BspFNI CGCG 1 cut(s) 39
BspHI TCATGA 1 cut(s) 4378
BspLI GGNNCC 8 cut(s) 1392, 1500, 1977, 2817, 3365, 4089, 4165, 4395
BspMI ACCTGC 1 cut(s) 2944
BspOI GCTAGC 1 cut(s) 4938
BspQI GCTCTTC 1 cut(s) 3676
BspT107I GGYRCC 1 cut(s) 1390
BspTI CTTAAG 2 cut(s) 740, 2020
BspTNI GGTCTC 1 cut(s) 3793
BsrDI GCAATG 4 cut(s) 73, 117, 2272, 2817
BsrI ACTGG 9 cut(s) 859, 1472, 1912, 1993, 2266, 2811, 3789, 4273, 4585
BssECI CCNNGG 7 cut(s) 230, 313, 1489, 2248, 3367, 3543, 3606
BssNAI GTATAC 1 cut(s) 4766
BssSI CACGAG 2 cut(s) 1350, 1944
BssT1I CCWWGG 4 cut(s) 313, 2248, 3543, 3606
Bst1107I GTATAC 1 cut(s) 4766
Bst2BI CACGAG 2 cut(s) 1350, 1944
Bst2UI CCWGG 7 cut(s) 1518, 1983, 2573, 2892, 3101, 3131, 3368
Bst6I CTCTTC 9 cut(s) 916, 1265, 2199, 2704, 3195, 3372, 3676, 3749, 3844
BstAFI CTTAAG 2 cut(s) 740, 2020
BstAPI GCANNNNNTGC 1 cut(s) 1397
BstBAI YACGTR 2 cut(s) 761, 4754
BstC8I GCNNGC 7 cut(s) 1138, 2027, 2904, 3786, 4073, 4484, 4936
BstDSI CCRYGG 1 cut(s) 1489
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 2 cut(s) 98, 1143
BstHHI GCGC 2 cut(s) 97, 1142
BstMAI GTCTC 8 cut(s) 38, 1262, 1370, 2231, 2663, 3793, 4046, 4389
BstMCI CGRYCG 1 cut(s) 4248
BstMWI GCNNNNNNNGC 7 cut(s) 614, 1054, 1397, 2621, 2975, 4198, 4358
BstNI CCWGG 7 cut(s) 1518, 1983, 2573, 2892, 3101, 3131, 3368
BstNSI RCATGY 3 cut(s) 1768, 2676, 2906
BstPAI GACNNNNGTC 2 cut(s) 2925, 4746
BstSCI CCNGG 9 cut(s) 229, 1516, 1981, 2571, 2890, 3099, 3129, 3366, 4089
BstSFI CTRYAG 4 cut(s) 2030, 2370, 2940, 3951
BstSLI GKGCMC 3 cut(s) 1393, 1805, 2062
BstSNI TACGTA 1 cut(s) 4754
BstUI CGCG 1 cut(s) 39
BstV2I GAAGAC 7 cut(s) 1213, 2372, 2637, 2664, 3356, 3597, 3876
BstX2I RGATCY 3 cut(s) 333, 4466, 4598
BstXI CCANNNNNNTGG 2 cut(s) 1207, 4071
BstYI RGATCY 3 cut(s) 333, 4466, 4598
BstZ17I GTATAC 1 cut(s) 4766
BsuI GTATCC 2 cut(s) 1341, 4173
BsuRI GGCC 7 cut(s) 1211, 2254, 2376, 3129, 3542, 3611, 4075
BtgI CCRYGG 1 cut(s) 1489
BtgZI GCGATG 1 cut(s) 3723
BtsI GCAGTG 2 cut(s) 223, 3481
BtuMI TCGCGA 1 cut(s) 39
BveI ACCTGC 1 cut(s) 2944
Cac8I GCNNGC 7 cut(s) 1138, 2027, 2904, 3786, 4073, 4484, 4936
CaiI CAGNNNCTG 2 cut(s) 53, 3795
CciI TCATGA 1 cut(s) 4378
CfoI GCGC 2 cut(s) 97, 1142
Cfr13I GGNCC 8 cut(s) 1230, 1976, 2252, 3164, 3547, 4087, 4164, 4393
CsiI ACCWGGT 2 cut(s) 2571, 2890
CspCI CAANNNNNGTGG 2 cut(s) 1471, 1506
DraI TTTAAA 1 cut(s) 1657
EaeI YGGCCR 2 cut(s) 1209, 4073
Eam1104I CTCTTC 9 cut(s) 916, 1265, 2199, 2704, 3195, 3372, 3676, 3749, 3844
EarI CTCTTC 9 cut(s) 916, 1265, 2199, 2704, 3195, 3372, 3676, 3749, 3844
Eco105I TACGTA 1 cut(s) 4754
Eco130I CCWWGG 4 cut(s) 313, 2248, 3543, 3606
Eco147I AGGCCT 1 cut(s) 3542
Eco31I GGTCTC 1 cut(s) 3793
Eco32I GATATC 1 cut(s) 3961
Eco47I GGWCC 7 cut(s) 1230, 1976, 3164, 3547, 4087, 4164, 4393
Eco57I CTGAAG 7 cut(s) 74, 489, 2136, 2384, 2640, 3758, 3872
Eco88I CYCGRG 1 cut(s) 4043
EcoO109I RGGNCCY 2 cut(s) 1976, 4087
EcoRI GAATTC 1 cut(s) 3721
EcoRII CCWGG 7 cut(s) 1516, 1981, 2571, 2890, 3099, 3129, 3366
EcoRV GATATC 1 cut(s) 3961
EcoT14I CCWWGG 4 cut(s) 313, 2248, 3543, 3606
EcoT22I ATGCAT 1 cut(s) 3051
ErhI CCWWGG 4 cut(s) 313, 2248, 3543, 3606
Esp3I CGTCTC 2 cut(s) 1262, 2231
FaqI GGGAC 4 cut(s) 1924, 3953, 4177, 4538
FauNDI CATATG 3 cut(s) 1396, 1432, 3271
FbaI TGATCA 3 cut(s) 220, 4297, 4432
FblI GTMKAC 2 cut(s) 4750, 4765
GlaI GCGC 2 cut(s) 96, 1141
GsuI CTGGAG 2 cut(s) 2462, 3817
HaeII RGCGCY 2 cut(s) 98, 1143
HaeIII GGCC 7 cut(s) 1211, 2254, 2376, 3129, 3542, 3611, 4075
HapII CCGG 3 cut(s) 230, 2768, 4091
HhaI GCGC 2 cut(s) 97, 1142
Hin6I GCGC 2 cut(s) 95, 1140
HinP1I GCGC 2 cut(s) 95, 1140
HincII GTYRAC 4 cut(s) 2346, 2569, 3358, 3419
HindII GTYRAC 4 cut(s) 2346, 2569, 3358, 3419
HindIII AAGCTT 7 cut(s) 1170, 1755, 2983, 3001, 3782, 4480, 4735
HpaI GTTAAC 1 cut(s) 2346
HpaII CCGG 3 cut(s) 230, 2768, 4091
HphI GGTGA 9 cut(s) 869, 1171, 1532, 2122, 2469, 2930, 4061, 4812, 4916
HpyCH4IV ACGT 5 cut(s) 760, 1242, 1255, 4446, 4753
HpyF10VI GCNNNNNNNGC 7 cut(s) 614, 1054, 1397, 2621, 2975, 4198, 4358
HpySE526I ACGT 5 cut(s) 760, 1242, 1255, 4446, 4753
HspAI GCGC 2 cut(s) 95, 1140
Kpn2I TCCGGA 1 cut(s) 2767
Ksp22I TGATCA 3 cut(s) 220, 4297, 4432
KspAI GTTAAC 1 cut(s) 2346
LguI GCTCTTC 1 cut(s) 3676
LmnI GCTCC 6 cut(s) 1498, 2137, 2176, 2620, 2815, 3112
MabI ACCWGGT 2 cut(s) 2571, 2890
MaeII ACGT 5 cut(s) 760, 1242, 1255, 4446, 4753
MfeI CAATTG 2 cut(s) 2852, 3412
MflI RGATCY 3 cut(s) 333, 4466, 4598
MhlI GDGCHC 6 cut(s) 1357, 1393, 1697, 1805, 2062, 2954
MlsI TGGCCA 1 cut(s) 4075
MluNI TGGCCA 1 cut(s) 4075
MmeI TCCRAC 5 cut(s) 560, 1131, 2246, 3206, 4808
Mox20I TGGCCA 1 cut(s) 4075
Mph1103I ATGCAT 1 cut(s) 3051
MroI TCCGGA 1 cut(s) 2767
MroXI GAANNNNTTC 5 cut(s) 322, 1281, 3632, 3725, 4841
MscI TGGCCA 1 cut(s) 4075
MslI CAYNNNNRTG 3 cut(s) 833, 1610, 1944
Msp20I TGGCCA 1 cut(s) 4075
MspCI CTTAAG 2 cut(s) 740, 2020
MspI CCGG 3 cut(s) 230, 2768, 4091
MspR9I CCNGG 9 cut(s) 231, 1518, 1983, 2573, 2892, 3101, 3131, 3368, 4091
MunI CAATTG 2 cut(s) 2852, 3412
Mva1269I GAATGC 3 cut(s) 265, 1939, 2720
MvaI CCWGG 7 cut(s) 1518, 1983, 2573, 2892, 3101, 3131, 3368
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 7 cut(s) 614, 1054, 1397, 2621, 2975, 4198, 4358
NciI CCSGG 2 cut(s) 231, 4091
NdeI CATATG 3 cut(s) 1396, 1432, 3271
NheI GCTAGC 1 cut(s) 4934
NlaIV GGNNCC 8 cut(s) 1392, 1500, 1977, 2817, 3365, 4089, 4165, 4395
NruI TCGCGA 1 cut(s) 39
NsiI ATGCAT 1 cut(s) 3051
NspI RCATGY 3 cut(s) 1768, 2676, 2906
OliI CACNNNNGTG 1 cut(s) 1610
PaeI GCATGC 1 cut(s) 2906
PaeR7I CTCGAG 1 cut(s) 4043
PagI TCATGA 1 cut(s) 4378
PceI AGGCCT 1 cut(s) 3542
PciI ACATGT 2 cut(s) 1764, 2672
PciSI GCTCTTC 1 cut(s) 3676
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 1248
PctI GAATGC 3 cut(s) 265, 1939, 2720
PdmI GAANNNNTTC 5 cut(s) 322, 1281, 3632, 3725, 4841
PflMI CCANNNNNTGG 4 cut(s) 579, 2261, 2554, 3795
PfoI TCCNGGA 1 cut(s) 3099
Ppu21I YACGTR 2 cut(s) 761, 4754
PpuMI RGGWCCY 2 cut(s) 1976, 4087
PscI ACATGT 2 cut(s) 1764, 2672
PshAI GACNNNNGTC 2 cut(s) 2925, 4746
PshBI ATTAAT 3 cut(s) 3456, 4020, 4550
PsiI TTATAA 3 cut(s) 381, 1106, 2450
Psp5II RGGWCCY 2 cut(s) 1976, 4087
Psp6I CCWGG 7 cut(s) 1516, 1981, 2571, 2890, 3099, 3129, 3366
PspGI CCWGG 7 cut(s) 1516, 1981, 2571, 2890, 3099, 3129, 3366
PspN4I GGNNCC 8 cut(s) 1392, 1500, 1977, 2817, 3365, 4089, 4165, 4395
PspPI GGNCC 8 cut(s) 1230, 1976, 2252, 3164, 3547, 4087, 4164, 4393
PspPPI RGGWCCY 2 cut(s) 1976, 4087
PstNI CAGNNNCTG 2 cut(s) 53, 3795
PsuI RGATCY 3 cut(s) 333, 4466, 4598
RruI TCGCGA 1 cut(s) 39
RseI CAYNNNNRTG 3 cut(s) 833, 1610, 1944
SapI GCTCTTC 1 cut(s) 3676
Sau96I GGNCC 8 cut(s) 1230, 1976, 2252, 3164, 3547, 4087, 4164, 4393
ScaI AGTACT 1 cut(s) 4373
ScrFI CCNGG 9 cut(s) 231, 1518, 1983, 2573, 2892, 3101, 3131, 3368, 4091
SduI GDGCHC 6 cut(s) 1357, 1393, 1697, 1805, 2062, 2954
SexAI ACCWGGT 2 cut(s) 2571, 2890
SfcI CTRYAG 4 cut(s) 2030, 2370, 2940, 3951
Sfr274I CTCGAG 1 cut(s) 4043
SinI GGWCC 7 cut(s) 1230, 1976, 3164, 3547, 4087, 4164, 4393
SlaI CTCGAG 1 cut(s) 4043
SmiMI CAYNNNNRTG 3 cut(s) 833, 1610, 1944
SmlI CTYRAG 7 cut(s) 740, 1891, 2020, 3279, 3428, 4043, 4702
SmoI CTYRAG 7 cut(s) 740, 1891, 2020, 3279, 3428, 4043, 4702
SnaBI TACGTA 1 cut(s) 4754
SpeI ACTAGT 1 cut(s) 4204
SphI GCATGC 1 cut(s) 2906
SseBI AGGCCT 1 cut(s) 3542
SsiI CCGC 4 cut(s) 2535, 3446, 4244, 4559
SspI AATATT 2 cut(s) 106, 355
StuI AGGCCT 1 cut(s) 3542
StyD4I CCNGG 9 cut(s) 229, 1516, 1981, 2571, 2890, 3099, 3129, 3366, 4089
StyI CCWWGG 4 cut(s) 313, 2248, 3543, 3606
TaiI ACGT 5 cut(s) 763, 1245, 1258, 4449, 4756
TaqI TCGA 9 cut(s) 19, 125, 182, 1115, 3294, 3636, 4044, 4248, 4608
TaqII GACCGA 1 cut(s) 3817
TatI WGTACW 4 cut(s) 699, 3039, 3670, 4371
TauI GCSGC 1 cut(s) 4562
TspGWI ACGGA 4 cut(s) 22, 2124, 2213, 4994
Van91I CCANNNNNTGG 4 cut(s) 579, 2261, 2554, 3795
Vha464I CTTAAG 2 cut(s) 740, 2020
VneI GTGCAC 1 cut(s) 1801
VpaK11BI GGWCC 7 cut(s) 1230, 1976, 3164, 3547, 4087, 4164, 4393
VspI ATTAAT 3 cut(s) 3456, 4020, 4550
XapI RAATTY 6 cut(s) 653, 861, 3073, 3721, 3857, 4908
XbaI TCTAGA 2 cut(s) 412, 1037
XceI RCATGY 3 cut(s) 1768, 2676, 2906
XcmI CCANNNNNNNNNTGG 1 cut(s) 2262
XhoI CTCGAG 1 cut(s) 4043
XmaJI CCTAGG 1 cut(s) 2248
XmiI GTMKAC 2 cut(s) 4750, 4765
XmnI GAANNNNTTC 5 cut(s) 322, 1281, 3632, 3725, 4841
ZrmI AGTACT 1 cut(s) 4373
Zsp2I ATGCAT 1 cut(s) 3051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.