RLG00000023099

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000223
Physical Location & Seq
Reverse (-)
18089570 .. 18100457
10888 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023099

Sequence Viewer

Length: 7779 bp
ATGGTGGGTTTACCTGAGACTGTGTTTTCTTGGTCCCTTGAAGACATTTTCAATGAAGATCTTTACAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCCGTCTGTTGTCCAGTACTTTGGGTCTTATCTGTACCCTTTATTGGAAGAAACTCGAGCTCAGTTACATTCCAGTATGGAAACTATTGACAGTGCACCATTTGCGGAAGTAGTTGATTTTAAAGAGTCCAAGCCATATGGAACAAAGCAATATACAATCAAGGTTGATTGTTGGCGAAACAGGTTCAGTGAACGTGGCAACGAACCATACAAAACGTTGCCTGGGGATCTTTTTATTTTAGCAAATGCTAAACCTGAAACTGTTTCTGAATTACAACTGGCTGGGAGGTCATGGTCTTTTGTATCTGTCACTCATCTTGTGTCTAACATCTCACAAATTCAGAAAAAATATGAGAATGGGGATGAGGATGAGGATGTGGAAGAGGAAACTTCTCTCTGTTTTAAAATCGAGTCTTCAAAAGAGTTTGAAGTTGATGATAGCACATCACTGTTTGTTGTTTTCTTGTTGAATTTAATCCCCAGTGGAAGAATATACAAAGCATTGCACATGCCGGGAAATTTAACAATTATCAAAGAAGTTCTATGCTCTGATTCTGTGGCTCAGGAAAATCCCTCCTATGAGAAGAATAATGACATCATGGAGAAGGGGTTAGATGAGAATTTATTGTCAGGACTTAATGAGGCCCAAACTGGGGCAGTTTTGGCTTGTCTTGGAATGCGGCATTGTGGTGCAAAGTCTTCGGTGGAACTTATATGGGGTCCTCCTGGGACAGGAAAAACTAAAACAATCGCTACTCTGCTTTTTACTCTGTTAAAGATGAATTGCAGGACTCTTATTTGTGCCCCAACAAATGTTGCAATAATTGAAGTTGCTTCTCGTCTTCTGAAAATGGTCACAGAAGCAGAGTCCGATGCCATGTTTCATTCAGTTGGAAAAATCCTTTTATTTGGGAATAAGGAACGACTCAAACTCAAAGTTGGTTCAGATATGGAAGAGATATATTTGGGACACCGTATTGAAAAAATCATCGAGTGCATAGGACCACAAACTGGTTGGAGGCATTGCTTTGCTTCCATGATAAATTTACTGAAGATTGTGTTTTTGACTGAACTCACTGAAGGGGAAGAACAAAATAGTGCTTGTGAAGTGAATGATGAATGCAGAAGTGAACCAGGAGTTAGTGAGGGGAAGTGCAAAACATTTCTTGAATATTTCAGAAATAGATTTCTCCCTACTGCATTGCCACTAAGATATTGCATTTCTATCTTCTGTACCCATATGACCAAAAGCTGCATTCCAGAACACATATTCAAGGATATGGTTTCTCTTATCCTCTTAGTTGATTCCTTTGAGATGTTGTTGTTTGAAGGTAATGTTATTTCAGAAGCATTGGAAGGCCTTTTTTCTTGTTCAGAGGTTGAGGGTGTATCTGAGTTATATGTAGATGACTCAATTCCTCTTGTCAGGAAAAGAAAGGAATGCCTTGTAGCTTTACGCACTCTACAGTATTCTCTTAGTAAACTTCAACTTCCAGATTTTAGGAAAGGAACTGAGCTAATGGATTTATGTTTCCAAGGTGCATCCTTAGTTTTTTGCACGGCATCTAGTTCATTTAAGCTGCATAGACTGGAAATGGAGCCATTTACCTTTGTGGTTCTTGATGAAGCTGCACAGTTGAAAGAGTGTGAGTCAACTATACCCCTGCAACTACCAGGAGTAAAACGTGTAATTCTTGTTGGTGATGAATGTCAATTGCCAGCTGTGGTGAAAAGCAATGTTTCTCAAGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCACAATGGGTCACCCAAAACACCTTCTAAATAAGCAGTACAGAATGCATCCGTCAATAAGTTTATTCCCAAATTCTTGTTTCTATAATAACCGGATCCTGGACGCTCCAAATGTCATAAAAAAAAGCTATGAGAAGCACTATCTTCCAGGGCCAATATATGGCCCTTATTCTTTTATAAATAATATGGTTGAGGTCGCTATTGTTTTGAAAGTACTGCAGAATCTTTACAAAGAATGGAAGAATTCAAAACAGAAACTCAGTGTTGGTGTGGTATCTCCCTATGCTGCTCAAGTAGTGGCAATTCAGGAAAATCTTAAACAAAAGTATGACAATCTTGATGGCTTAAAAGTGAAGGTAAATACAGTTGATGGGTTCCAGGGTGGGGAAGACGACATAATTATAATGTCCACTGTAAGATCCAACAGCCATCAATCACTTCAGTTTATTTCAAATATTCAAAGAGTCAATGTTGCTCTCACAAGGGCTAGGCACTGTCTCTGGATTTTGGGCAACGAAAAAACTCTATGCAGTACTGATTCTGTTTGGAAGGCCTTGGTTCTTGATGCCAAAAGTCGTGAATGCTTCTTTAATGCTGATCAAGACAAGGATTTGGCCAAGGCCATAGTAGACGTAAAGAAAGAGTTGGACCAATTGGATGATTTGCTCAATGCCAACAGCATACTTTTCAAAAATGCCAGATGGAAGGTTCTTTTTAGTGATAATTTTTTGAAGTCATTCAAGAAACTGACGTCAGTCTCTTTGAAGAAGTCAGTACTAAACCTTCTACTGAAGCTTTCAGCTGGCTGGAGACCTAGGAAGCGCAATGTAGAGACAGGTTGTAGTAGCTCTTCACTAATCTTGAAGAAATTTAAGATTAAATCTCTATATATTGTTTGCACAACAGACATTGCAAAGGATATTGTTTGCACAACAGACACTGCAAAGGATATGAGATACATTCAAATATTAAAGGTCTGGGACATATTGCTCCTGGAGGATATCCCAAAGTTGGAATATCGCCCGGAGACTATTCTAAACAGATATACAGATGATTTTATTAGTCTTTGCAAGGAGAAATATCTTGATGGTGATTTAGAAGTTCCACAGACCTGGCCACTTTCTCTGGAATTTTCCCAGTTCAAGGATCCTAGTGTCACTGAAACCAAGAGAGATTTTGTTGGTGACACTTCTGATGGCAGGAGTTATGTTGAGAATTCACAGGTCAGTGAGAGTTTGTTGCTAATGAAATTTTACTCTTTATCATCTGGTTTGGTGAACCACTTGCTCTCTGACTCTGGGGGTAGAGAATTGGATCTCCCGTTTGAAGTTACAGACCAAGAAAGGGAGATTATACTTCATCATAGAAGTTCTTTTATAGTTGGACGGTCAGGTACTGGGAAGACCACCGTCTTAACCATGAAGTTATTCCAAAGAGAACAATGGCACCATTTGGCTGTGCAGGGATGTTATGGTACTGAAAATAGCAATGATGAGCAAAGTTCTGCAACTACTGAAGGGAAAATTCTACGCCAGCTTTTCGTTACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCAACATGTTACACATTTGAAAAGTTTTGCCTGTGGTGGAAGTGAATCAGCTGAAAGAAGTTTGATTGATATAGTTGATTTTGAAGAGGAGGAATCCCAATTCAAGGATATCCAAGATTCATTTCATGATATTCCTCCGAGTTCTTACCCACTTGTGATAACATTCTATAAGTTCCTGATGATGCTTGACGGAACATTGAGTAACTCATACTTTGCAAGGTTCCGCGATGCGTCACTTCCTCATGGCCAATTTAGAAATTCGAGATCAGTTGTGTTCCAGGCCTTTATAAGGACAAAAGAAGTCAATTATGAGAGGTTTAGTTCATCATATTGGCCCCATTTTAATACTCAGCTAACTAAGAACCTTGATGTTGCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGCTTGGGAACAATAGAAGCATGTGATGGGAAGCTCAGCCGGGAGGATTATGTTAAATTGTCAGAGGGCCGAGCTTCTAATTTAAGCAAGCAAAAGAGAGAACAGATATACGAGATATTTCAAGATTATGAAAAAATGAAGATGCGAAATGGTGAATTTGATCTGGCTGATTTTGTAAATGATCTTCAACGTCAACTCAAGCATGAGAAATATATCGGTGATCAGATGGATTTTATTTATATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTCAAACATATGTGCAACAATGTTGATGAGGGATTTGTTTTTTCTGGTGATACAGCACAAACAATTGCCAGGGGTATTGATTTTAGGTTCCAAGATATACGACATCTGTTCTACAAGACGTTTGTGTTGGAGTCTAGGAGCAATAAGCAAGATGAAAGAAAAGGAAAAGGCCAAATTTCTGAAATATATCATTTGACTCAAAACTTCCGTACCCATTCTGGTATATTGAAGTTATCGCAGAGCATTATTGAATTACTATATCGCTTTTTTCCTCTTTCAATTGATGTTTTAAAACCAGAATGCAGTCTGATTTATGGGGAAGCTCCGGTTGTGCTTGAATCTGGAGAAAATGAAAATGCAATCATCAAAATATTTGGAAATAGTAGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATATTGGTGCGAGATGATAGTACTCGAAGAGAAATTTCCAATGTTGTTGGGAAGCATGCTCTTGTCCTTACCATAGTTGAGTGCAAGGGCCTTGAGTTTCAGGATGTACTCTTGTACAACTTTTTCGGCTCATCTCCTTCGAAAAATCAATGGAGGGTTATTTATGATTACATGAAGGAACAAGATTTACTCGACTCTACATTACCTAAGAAATTTCCAAGTTTCAGTGAAGCGAAACACAACATATTATGCTCTGAATTGAAGCAATTATATGTTGCTGTTACTCGCACCAGACAGAGATTGTGGATTTGTGAAACTGTAGAGGAGATTTCGAAACCCATGTTTGACTATTGGAAGAAGAAATGCCTTGTGCAAGTTAGGCAACTTGATGATTCACTTGCACAAGCCATGCAAGTTGCAAGCAGCACAGAGGAGTGGAGATCCCGTGGGATTAAGCTATATCGTGAGAATAACTATGAGATGGCCACAATGTGCTTTAAAAGATCTGGTGACACTTATTGGGAACAAAGGTCCAAAGCTGCTGGCCTTAAAGCTACTGCTGACCGTATGCGAATGTCAAATCCTGAAGAGGCTAATTCTATTCTTAGGAAAGCTGCTGAAATATTTGATGCTATAGGGAAGGCTGATTCTGCTGCAAGATGTTTTTCTGATTTGGGGGAGTATGAAAGAGCAGCCAGGATTTATTTTGAGAAATGTGGTGAATTGGAAAGAGCAGCTGAATGTTTCACTCTAGCAGGATGCTATGAGGATGCTGCAGATGTGTATGCTAAAGGCAATTTTTTCGTAGAGTGTCTCTCTGTCTGTGCCAAAGGAAAATTATTTGATAGAGGTTTGGAATACATCGATTATTGGAAGAGGCATGCAGTAGAGAACAATTCTTTAACTAGAGGAGGAGAGGGGATACATAACATGGAACAAAAATTTCTAGAGGGCTGTGCACGCCACTATCATAAGATGAAAGATAACAGATCCATGATGAAATTTGTAAAAGCCTTCCGGTCTATATCATTGATACGGAATTTTTTGAAGGGTTTAGGTAGCCTTGATGAACTTCTTTTGCCGGAAGATCAATTTGGAAACTATTTGGAAGCTGCGGAGATTGCACAGATTAAAGGCGAAATTCTACTTAAATCGGATTATCTTGGAAAGGCAGGTGAATTCAAAGATGCCTCAATGCATGTCCTATTCTATGTACTCGCCAGCTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCCATGAAGTTTGCACAAAAGGAAGCTCTTTTGACAAAAGCAAAGTCAGTTGCCAAGAATGAGACAGACGACTTTTATGAGTTGGTTTGCACTGAGGTTGATATTTTATTAAATGGGCAGGATAATTTGGCTGTGTTGAAAGATCAAATGAAGGCTTCTCAGAGACATGCAAGTATTATAGGTGAAGTGTTATCTGCTCGAAAAATGTTGGATTCCCATTTTTCTCTGTGGACTGATAAGTATTTGTGGGAAAAAGAATTGATCAGTGATTTTGTGAAGCATTCTGAAGACATGATATCCCAAAACCAGGTTTCCATCGAATCACTGGTTTACTTCTGGAATTTTTGGAAGGAAAAGATTGTTCTCGTTATTGAATGTCTAGGATGCCTTGAAACCCAAGATGTTAATGTAAACAGAAGTTATCAAAAGTTTTGTTTAAACTACTTGGGAGTATGGAGGCTATCTAAACAAATGAATCCTGTGTACGTTTTACTCTTCCCTGATGCTGATTGGGTCAGAGATGTGGACAAAAGTTATTTCCAAAGTCATGGGAAGTTGGTCTCCATTGATGTTCGCCAGCTTATTTCAGCTGCTCAGAAATATTGGAGTTCGGAACTGCTTGCTGTAGGCATTATGGTTTTGGAAAAGCTTGATGAGCTTTCCAAGTTCTCTATCAAGAGTTCTGATTCAACATTCTTCCGGAGCAGGGCTCTTACCTTCATCTATGATGTTGCGGTGTATCTCTTGAATTCCAAATGTTTGAAGAGAAGGCCTCAAGATGCCAAGGCACTGCAGAAATTTGTAACTTTCTCAACTGAAAGGTTTGTTGCTTGCCTGTTTCCTTTAGATTGGAGGGAATCATTTAGAGAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATACATCAGCTCAAAGAACGAGCTGTCTTATGGTCAAATTGGAAGGATCGCAATGACCATTCTCGGGTCTGGTAAGCTGAATTTTGAACTATGTAAGAAGCTTGTGAAAAAGTTGGACTCCAATCCACCATGGAAGGACTTTATTGAGAACCTATGTGCGAATACTGAACCTGGAAATACCAGTCAGAAGCCTAGAGAGGTGTCTGTTATGTGGCTATTATCTGAAGCTTTAAATGAAACTTATAATGCAAACTGGAGGGTTGTTAAAGACTACATATCACCTGGTTGCTTCTTGTATCTTGTGGAGCGTCTTCTGATTTGGGCTTCTCAATGTCGAGGTTATGTTATCACCACAAGTTCTTGTTTCACTGAATGGCTGATATACCAGGATGAAGATACTAATGTGGAACCATCTATAGAAGGCATCCTTCATTTTGTTAGTAATGTGGTTATCGAATGCCTTTGTAACAGGGCAAATCTGATTGAATGGATTAAAAAGTCTAATGCAGATTGGAAGGCCTGTTATTCACTACTCGTGCCAAGATTGGTGTTTGTACTATGTTTGCTTTGTGTGAACTTTGGAATATGCTTGGATGTACTGTCTGATATGCTGGGAAGGAGCTATATCACTGAACAACTTCCATGGGAATTTTACGACACCCTTAAGAGGCGGAGGAGGAACAATTTCCTGAATATAAATGTGGAAGTGCTTGCAGCAGCTCTCAAGAAGATTCATAATCCTTTGGTAATTGCGGGTTTTGGGTTTGATTGTTCAAGGTTCGTATGCCCAGATGCCATCTTTGTTGACATGAAGGCCATTCAGTCCAAGGATGACCTATTCAGAATAATGTTTCCTAAACCACCAGCCATTCAAGTTGCACCAGGTCGAATTGGATTTGTTGAAGCTGGAGGTAATAGTCTTTGTGGTGATGTACTTTCTGCAAACGATACCGAAGCAGGGAAGAGCTCTTTAAACTCCAGCTTGCTGGGTGAAACTGATGAGCAGTTTGATACTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2593

Amino Acids

295.72

Weight (kDa)

5.97

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 66 - 207 2.3e-48 Domain of unknown function (DUF6469)
AAA_11 PF13086 245 - 612 8.4e-33 AAA domain
AAA_19 PF13245 265 - 608 6.8e-09 AAA domain
AAA_12 PF13087 620 - 800 5.7e-47 AAA domain
UvrD-helicase PF00580 1316 - 1406 1.3e-08 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 2060, 2285, 3746, 6999
AarI CACCTGC 2 cut(s) 4555, 5732
AatII GACGTC 1 cut(s) 2636
Acc36I ACCTGC 2 cut(s) 4555, 5732
AccB1I GGYRCC 1 cut(s) 3327
AccB7I CCANNNNNTGG 2 cut(s) 1109, 2042
AccI GTMKAC 1 cut(s) 2511
AccII CGCG 1 cut(s) 3684
AccIII TCCGGA 1 cut(s) 6522
AciI CCGC 7 cut(s) 203, 778, 3682, 5684, 6557, 7426, 7508
AclI AACGTT 1 cut(s) 314
AcoI YGGCCR 4 cut(s) 2496, 2996, 3703, 5052
AcuI CTGAAG 8 cut(s) 1169, 1197, 2306, 2693, 3417, 5175, 6129, 6999
AcyI GRCGYC 1 cut(s) 2633
AdeI CACNNNGTG 1 cut(s) 5061
AflII CTTAAG 1 cut(s) 7418
AflIII ACRYGT 2 cut(s) 1783, 3463
AjuI GAANNNNNNNTTGG 4 cut(s) 3564, 3596, 5646, 5678
Alw21I GWGCWC 4 cut(s) 160, 196, 5530, 7724
Alw44I GTGCAC 2 cut(s) 192, 5526
AlwNI CAGNNNCTG 2 cut(s) 2822, 3278
Ama87I CYCGRG 2 cut(s) 153, 6818
Aor13HI TCCGGA 1 cut(s) 6522
ApaLI GTGCAC 2 cut(s) 192, 5526
ArsI GACNNNNNNTTYG 2 cut(s) 5852, 5884
Asp700I GAANNNNTTC 6 cut(s) 84, 96, 2618, 3308, 7392, 7439
AspA2I CCTAGG 1 cut(s) 2696
AspLEI GCGC 1 cut(s) 2706
AsuC2I CCSGG 3 cut(s) 612, 2906, 3911
AsuII TTCGAA 2 cut(s) 4709, 4901
AvaI CYCGRG 2 cut(s) 153, 6818
AvaII GGWCC 5 cut(s) 33, 818, 1100, 2530, 5100
AvrII CCTAGG 1 cut(s) 2696
BaeGI GKGCMC 3 cut(s) 196, 904, 5530
BaeI ACNNNNGTAYC 4 cut(s) 4345, 4378, 7143, 7176
BalI TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
BamHI GGATCC 2 cut(s) 1977, 3028
BanI GGYRCC 1 cut(s) 3327
BanII GRGCYC 3 cut(s) 160, 6535, 7724
BarI GAAGNNNNNNTAC 6 cut(s) 2649, 2681, 2716, 2748, 4038, 4070
BauI CACGAG 1 cut(s) 7289
BbsI GAAGAC 8 cut(s) 48, 504, 789, 932, 2277, 3290, 6117, 7058
Bbv12I GWGCWC 4 cut(s) 160, 196, 5530, 7724
BceAI ACGGC 1 cut(s) 1674
BcgI CGANNNNNNTGC 4 cut(s) 780, 814, 5353, 5387
BciVI GTATCC 1 cut(s) 5484
BclI TGATCA 3 cut(s) 2479, 4090, 6084
BcnI CCSGG 3 cut(s) 612, 2906, 3911
BfmI CTRYAG 8 cut(s) 1562, 2099, 4887, 5202, 5343, 6447, 6614, 7170
BfrI CTTAAG 1 cut(s) 7418
BfuAI ACCTGC 2 cut(s) 4555, 5732
BfuI GTATCC 1 cut(s) 5484
BglII AGATCT 3 cut(s) 58, 4126, 5072
BlnI CCTAGG 1 cut(s) 2696
BlpI GCTNAGC 1 cut(s) 3905
BmcAI AGTACT 5 cut(s) 116, 2097, 2416, 2658, 4591
Bme18I GGWCC 5 cut(s) 33, 818, 1100, 2530, 5100
BmeT110I CYCGRG 2 cut(s) 153, 6818
BmrI ACTGGG 4 cut(s) 573, 759, 3013, 3288
BmuI ACTGGG 4 cut(s) 573, 759, 3013, 3288
BoxI GACNNNNGTC 2 cut(s) 2636, 3290
BpiI GAAGAC 8 cut(s) 48, 504, 789, 932, 2277, 3290, 6117, 7058
BplI GAGNNNNNCTC 6 cut(s) 5369, 5401, 6517, 6549, 6580, 6612
BpmI CTGGAG 6 cut(s) 2710, 2897, 4515, 7030, 7683, 7717
Bpu10I CCTNAGC 2 cut(s) 660, 3438
Bpu1102I GCTNAGC 1 cut(s) 3905
Bpu14I TTCGAA 2 cut(s) 4709, 4901
BpuEI CTTGAG 6 cut(s) 1827, 2157, 4052, 4682, 6582, 7463
BpuMI CCSGG 3 cut(s) 612, 2906, 3911
Bsa29I ATCGAT 1 cut(s) 5433
BsaHI GRCGYC 1 cut(s) 2633
BsaI GGTCTC 2 cut(s) 2686, 6388
BsaWI WCCGGW 4 cut(s) 1974, 4477, 5586, 6522
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 4999, 5029
Bse3DI GCAATG 8 cut(s) 599, 1120, 1298, 1840, 2713, 2790, 3376, 6813
BseAI TCCGGA 1 cut(s) 6522
BseCI ATCGAT 1 cut(s) 5433
BseMI GCAATG 8 cut(s) 599, 1120, 1298, 1840, 2713, 2790, 3376, 6813
BseRI GAGGAG 6 cut(s) 3560, 4907, 5015, 5493, 5496, 7444
BseSI GKGCMC 3 cut(s) 196, 904, 5530
BseYI CCCAGC 3 cut(s) 380, 7366, 7741
BsgI GTGCAG 3 cut(s) 1713, 3362, 4578
Bsh1236I CGCG 1 cut(s) 3684
BshNI GGYRCC 1 cut(s) 3327
BshVI ATCGAT 1 cut(s) 5433
BsiHKAI GWGCWC 4 cut(s) 160, 196, 5530, 7724
BsiHKCI CYCGRG 2 cut(s) 153, 6818
BsiSI CCGG 8 cut(s) 611, 1975, 2906, 3910, 4478, 5587, 5651, 6523
BslFI GGGAC 4 cut(s) 19, 841, 1080, 2876
BsmFI GGGAC 4 cut(s) 19, 841, 1080, 2876
BsmI GAATGC 9 cut(s) 780, 1223, 1353, 1544, 1932, 2468, 4457, 6103, 7217
Bso31I GGTCTC 2 cut(s) 2686, 6388
BsoBI CYCGRG 2 cut(s) 153, 6818
Bsp119I TTCGAA 2 cut(s) 4709, 4901
Bsp1286I GDGCHC 6 cut(s) 160, 196, 904, 5530, 6535, 7724
Bsp13I TCCGGA 1 cut(s) 6522
Bsp1407I TGTACA 1 cut(s) 4683
Bsp1720I GCTNAGC 1 cut(s) 3905
Bsp19I CCATGG 2 cut(s) 6884, 7397
BspACI CCGC 7 cut(s) 203, 778, 3682, 5684, 6557, 7426, 7508
BspDI ATCGAT 1 cut(s) 5433
BspEI TCCGGA 1 cut(s) 6522
BspFNI CGCG 1 cut(s) 3684
BspHI TCATGA 1 cut(s) 3583
BspMAI CTGCAG 3 cut(s) 2103, 5347, 6618
BspMI ACCTGC 2 cut(s) 4555, 5732
BspQI GCTCTTC 2 cut(s) 2737, 7712
BspT104I TTCGAA 2 cut(s) 4709, 4901
BspT107I GGYRCC 1 cut(s) 3327
BspTI CTTAAG 1 cut(s) 7418
BspTNI GGTCTC 2 cut(s) 2686, 6388
BsrDI GCAATG 8 cut(s) 599, 1120, 1298, 1840, 2713, 2790, 3376, 6813
BsrGI TGTACA 1 cut(s) 4683
BssNI GRCGYC 1 cut(s) 2633
BssSI CACGAG 1 cut(s) 7289
BssT1I CCWWGG 8 cut(s) 1633, 2436, 2499, 2696, 6606, 6884, 7397, 7581
Bst2BI CACGAG 1 cut(s) 7289
BstACI GRCGYC 1 cut(s) 2633
BstAFI CTTAAG 1 cut(s) 7418
BstAPI GCANNNNNTGC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 4683
BstBI TTCGAA 2 cut(s) 4709, 4901
BstDSI CCRYGG 3 cut(s) 5014, 6884, 7397
BstEII GGTNACC 1 cut(s) 1892
BstENI CCTNNNNNAGG 2 cut(s) 828, 3745
BstFNI CGCG 1 cut(s) 3684
BstHHI GCGC 1 cut(s) 2706
BstMWI GCNNNNNNNGC 9 cut(s) 200, 761, 1856, 4948, 5219, 5529, 5690, 5817, 6478
BstNSI RCATGY 7 cut(s) 610, 3467, 3894, 4628, 5453, 5771, 5993
BstPAI GACNNNNGTC 2 cut(s) 2636, 3290
BstPI GGTNACC 1 cut(s) 1892
BstSFI CTRYAG 8 cut(s) 1562, 2099, 4887, 5202, 5343, 6447, 6614, 7170
BstSLI GKGCMC 3 cut(s) 196, 904, 5530
BstUI CGCG 1 cut(s) 3684
BstV2I GAAGAC 8 cut(s) 48, 504, 789, 932, 2277, 3290, 6117, 7058
BstXI CCANNNNNNTGG 4 cut(s) 119, 2994, 6371, 7741
Bsu15I ATCGAT 1 cut(s) 5433
BsuI GTATCC 1 cut(s) 5484
BsuTUI ATCGAT 1 cut(s) 5433
BtgI CCRYGG 3 cut(s) 5014, 6884, 7397
BtgZI GCGATG 1 cut(s) 3699
BtsI GCAGTG 2 cut(s) 2820, 6611
BveI ACCTGC 2 cut(s) 4555, 5732
CaiI CAGNNNCTG 2 cut(s) 2822, 3278
CciI TCATGA 1 cut(s) 3583
CfoI GCGC 1 cut(s) 2706
ClaI ATCGAT 1 cut(s) 5433
CseI GACGC 3 cut(s) 1994, 3678, 7052
CsiI ACCWGGT 3 cut(s) 6129, 7036, 7636
DraI TTTAAA 7 cut(s) 220, 502, 4443, 5068, 6261, 6987, 7728
DraIII CACNNNGTG 1 cut(s) 5061
EaeI YGGCCR 4 cut(s) 2496, 2996, 3703, 5052
EciI GGCGGA 1 cut(s) 7441
Ecl136II GAGCTC 2 cut(s) 158, 7722
Eco130I CCWWGG 8 cut(s) 1633, 2436, 2499, 2696, 6606, 6884, 7397, 7581
Eco147I AGGCCT 5 cut(s) 1458, 2435, 3740, 6595, 7274
Eco24I GRGCYC 3 cut(s) 160, 6535, 7724
Eco31I GGTCTC 2 cut(s) 2686, 6388
Eco32I GATATC 3 cut(s) 2884, 3568, 6120
Eco47I GGWCC 5 cut(s) 33, 818, 1100, 2530, 5100
Eco53kI GAGCTC 2 cut(s) 158, 7722
Eco57I CTGAAG 8 cut(s) 1169, 1197, 2306, 2693, 3417, 5175, 6129, 6999
Eco88I CYCGRG 2 cut(s) 153, 6818
Eco91I GGTNACC 1 cut(s) 1892
EcoICRI GAGCTC 2 cut(s) 158, 7722
EcoNI CCTNNNNNAGG 2 cut(s) 828, 3745
EcoO109I RGGNCCY 2 cut(s) 818, 4657
EcoO65I GGTNACC 1 cut(s) 1892
EcoRI GAATTC 4 cut(s) 2125, 3097, 5747, 6571
EcoRV GATATC 3 cut(s) 2884, 3568, 6120
EcoT14I CCWWGG 8 cut(s) 1633, 2436, 2499, 2696, 6606, 6884, 7397, 7581
EcoT22I ATGCAT 2 cut(s) 1932, 5769
EcoT38I GRGCYC 3 cut(s) 160, 6535, 7724
ErhI CCWWGG 8 cut(s) 1633, 2436, 2499, 2696, 6606, 6884, 7397, 7581
FalI AAGNNNNNCTT 2 cut(s) 7167, 7199
FaqI GGGAC 4 cut(s) 19, 841, 1080, 2876
FauI CCCGC 1 cut(s) 7501
FauNDI CATATG 3 cut(s) 235, 1338, 4161
FbaI TGATCA 3 cut(s) 2479, 4090, 6084
FblI GTMKAC 1 cut(s) 2511
FriOI GRGCYC 3 cut(s) 160, 6535, 7724
GlaI GCGC 1 cut(s) 2705
GsaI CCCAGC 3 cut(s) 384, 7370, 7745
GsuI CTGGAG 6 cut(s) 2710, 2897, 4515, 7030, 7683, 7717
HapII CCGG 8 cut(s) 611, 1975, 2906, 3910, 4478, 5587, 5651, 6523
HgaI GACGC 3 cut(s) 1994, 3678, 7052
HhaI GCGC 1 cut(s) 2706
Hin1I GRCGYC 1 cut(s) 2633
Hin6I GCGC 1 cut(s) 2704
HinP1I GCGC 1 cut(s) 2704
HincII GTYRAC 3 cut(s) 1752, 4064, 7561
HindII GTYRAC 3 cut(s) 1752, 4064, 7561
HindIII AAGCTT 5 cut(s) 1864, 2675, 6470, 6854, 6981
HpaII CCGG 8 cut(s) 611, 1975, 2906, 3910, 4478, 5587, 5651, 6523
HpyCH4IV ACGT 8 cut(s) 292, 314, 1783, 2514, 2633, 4060, 4271, 6309
HpyF10VI GCNNNNNNNGC 9 cut(s) 200, 761, 1856, 4948, 5219, 5529, 5690, 5817, 6478
HpySE526I ACGT 8 cut(s) 292, 314, 1783, 2514, 2633, 4060, 4271, 6309
Hsp92I GRCGYC 1 cut(s) 2633
HspAI GCGC 1 cut(s) 2704
Kpn2I TCCGGA 1 cut(s) 6522
Ksp22I TGATCA 3 cut(s) 2479, 4090, 6084
LguI GCTCTTC 2 cut(s) 2737, 7712
LmnI GCTCC 8 cut(s) 1696, 1993, 2877, 4290, 4480, 6525, 7060, 7374
MabI ACCWGGT 3 cut(s) 6129, 7036, 7636
MaeII ACGT 8 cut(s) 292, 314, 1783, 2514, 2633, 4060, 4271, 6309
MfeI CAATTG 4 cut(s) 1811, 2534, 4215, 4431
MhlI GDGCHC 6 cut(s) 160, 196, 904, 5530, 6535, 7724
MlsI TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
MluNI TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
MmeI TCCRAC 9 cut(s) 970, 1094, 2328, 2508, 2874, 3244, 4260, 6012, 6849
Mox20I TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
Mph1103I ATGCAT 2 cut(s) 1932, 5769
MroI TCCGGA 1 cut(s) 6522
MroXI GAANNNNTTC 6 cut(s) 84, 96, 2618, 3308, 7392, 7439
MscI TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
MslI CAYNNNNRTG 1 cut(s) 786
Msp20I TGGCCA 4 cut(s) 2498, 2998, 3705, 5054
MspA1I CMGCKG 5 cut(s) 1820, 2684, 3509, 5306, 6413
MspCI CTTAAG 1 cut(s) 7418
MspI CCGG 8 cut(s) 611, 1975, 2906, 3910, 4478, 5587, 5651, 6523
MssI GTTTAAAC 1 cut(s) 6261
MunI CAATTG 4 cut(s) 1811, 2534, 4215, 4431
Mva1269I GAATGC 9 cut(s) 780, 1223, 1353, 1544, 1932, 2468, 4457, 6103, 7217
MvnI CGCG 1 cut(s) 3684
MwoI GCNNNNNNNGC 9 cut(s) 200, 761, 1856, 4948, 5219, 5529, 5690, 5817, 6478
NciI CCSGG 3 cut(s) 612, 2906, 3911
NcoI CCATGG 2 cut(s) 6884, 7397
NdeI CATATG 3 cut(s) 235, 1338, 4161
NmeAIII GCCGAG 1 cut(s) 3965
NmuCI GTSAC 7 cut(s) 406, 952, 1892, 3037, 3065, 3690, 5078
NsiI ATGCAT 2 cut(s) 1932, 5769
NspI RCATGY 7 cut(s) 610, 3467, 3894, 4628, 5453, 5771, 5993
NspV TTCGAA 2 cut(s) 4709, 4901
PaeI GCATGC 2 cut(s) 4628, 5453
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 1 cut(s) 3583
PaqCI CACCTGC 2 cut(s) 4555, 5732
PceI AGGCCT 5 cut(s) 1458, 2435, 3740, 6595, 7274
PciI ACATGT 1 cut(s) 3463
PciSI GCTCTTC 2 cut(s) 2737, 7712
PctI GAATGC 9 cut(s) 780, 1223, 1353, 1544, 1932, 2468, 4457, 6103, 7217
PdmI GAANNNNTTC 6 cut(s) 84, 96, 2618, 3308, 7392, 7439
PflMI CCANNNNNTGG 2 cut(s) 1109, 2042
PfoI TCCNGGA 2 cut(s) 1980, 2874
PmeI GTTTAAAC 1 cut(s) 6261
PpuMI RGGWCCY 1 cut(s) 818
PscI ACATGT 1 cut(s) 3463
PshAI GACNNNNGTC 2 cut(s) 2636, 3290
PsiI TTATAA 4 cut(s) 2060, 2285, 3746, 6999
Psp124BI GAGCTC 2 cut(s) 160, 7724
Psp1406I AACGTT 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 818
PspEI GGTNACC 1 cut(s) 1892
PspFI CCCAGC 3 cut(s) 380, 7366, 7741
PspPPI RGGWCCY 1 cut(s) 818
PspXI VCTCGAGB 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 4740, 4772
PstI CTGCAG 3 cut(s) 2103, 5347, 6618
PstNI CAGNNNCTG 2 cut(s) 2822, 3278
PvuII CAGCTG 5 cut(s) 1820, 2684, 3509, 5306, 6413
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 2 cut(s) 160, 7724
SapI GCTCTTC 2 cut(s) 2737, 7712
ScaI AGTACT 5 cut(s) 116, 2097, 2416, 2658, 4591
SduI GDGCHC 6 cut(s) 160, 196, 904, 5530, 6535, 7724
SexAI ACCWGGT 3 cut(s) 6129, 7036, 7636
SfcI CTRYAG 8 cut(s) 1562, 2099, 4887, 5202, 5343, 6447, 6614, 7170
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 4709, 4901
SinI GGWCC 5 cut(s) 33, 818, 1100, 2530, 5100
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 8 cut(s) 153, 1842, 2172, 4067, 4661, 6597, 7418, 7478
SmoI CTYRAG 8 cut(s) 153, 1842, 2172, 4067, 4661, 6597, 7418, 7478
SphI GCATGC 2 cut(s) 4628, 5453
SseBI AGGCCT 5 cut(s) 1458, 2435, 3740, 6595, 7274
SsiI CCGC 7 cut(s) 203, 778, 3682, 5684, 6557, 7426, 7508
SspI AATATT 6 cut(s) 1271, 2338, 2850, 4524, 5193, 6425
SstI GAGCTC 2 cut(s) 160, 7724
StuI AGGCCT 5 cut(s) 1458, 2435, 3740, 6595, 7274
StyI CCWWGG 8 cut(s) 1633, 2436, 2499, 2696, 6606, 6884, 7397, 7581
TaiI ACGT 8 cut(s) 295, 317, 1786, 2517, 2636, 4063, 4274, 6312
TauI GCSGC 1 cut(s) 781
TseFI GTSAC 7 cut(s) 406, 952, 1892, 3037, 3065, 3690, 5078
Tsp45I GTSAC 7 cut(s) 406, 952, 1892, 3037, 3065, 3690, 5078
TspGWI ACGGA 5 cut(s) 90, 1923, 3663, 4349, 5620
Van91I CCANNNNNTGG 2 cut(s) 1109, 2042
Vha464I CTTAAG 1 cut(s) 7418
VneI GTGCAC 2 cut(s) 192, 5526
VpaK11BI GGWCC 5 cut(s) 33, 818, 1100, 2530, 5100
XagI CCTNNNNNAGG 2 cut(s) 828, 3745
XbaI TCTAGA 1 cut(s) 5515
XceI RCATGY 7 cut(s) 610, 3467, 3894, 4628, 5453, 5771, 5993
XcmI CCANNNNNNNNNTGG 2 cut(s) 1110, 6145
XhoI CTCGAG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 2696
XmiI GTMKAC 1 cut(s) 2511
XmnI GAANNNNTTC 6 cut(s) 84, 96, 2618, 3308, 7392, 7439
ZraI GACGTC 1 cut(s) 2634
ZrmI AGTACT 5 cut(s) 116, 2097, 2416, 2658, 4591
Zsp2I ATGCAT 2 cut(s) 1932, 5769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.