Rroxscaffold_5G00386370

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000223
Physical Location & Seq
Forward (+)
65265335 .. 65270430
5096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386370.1

Sequence Viewer

Length: 3714 bp
ATGAAAGTTATGTTCAAAATTCAAAAGGTCAGTGAGAGTTTGTTGCTGATGAAATTCTACTCCTTATCATCTGGGGTGGTGAATCACTTGCTCTCTGACCGTGAGGGTAGAGAGTTGGTTCTCCCATTTGAAGTTACTGATGAAGAAATGGAGGCCATACTTTACAATAGAAGTGCCTTCATAGTTGGGCGATCAGGCACTGGGAAGACCACTGTTTTAACAATGAAGTTATATCAGAAGGAACAGTATTACAATATGGCAAAGGAAGGATTGTATGCTGTCGAGCAAAGTTCTGGGGGTTCTAATGCAACTGTTTTAAGCCAGATATTTGTAACAGTTAGTCCAAAACTCTGTTTTGCCACCAAGCAACATGTATCGAACTTGAGAAGTTTTGCTTCTGGTGTAAGCTGCCATTCAGCTGAATTCGGCTCGATTGGTCTGGATGATTGTGATGATGCAGAAGCAGAATTTTGGGATCTCCCAAATTCATTTCTTGATATTCCTTCCGAGCATTATCCTCTTTTTATAACATTCCATAAATTTTTAATGATGCTGGATGGAAGTTTGAGTAATTCATACTTTGAAAGATTCCTTGACAACCTAAAACTACCTCGAAGTCCACCACAAAGTTCAAGATTGCTGAAAAGCTTATTGAGGACAAAGGAGGTCAATTACGAAAGGTTTTGTTCATCATATTGGCCTCATTTTAATATGAAGTTAACGAAGCGGCTTGATGCCTTGAGAGTCTTTACGGAGATTATTTATCATATCAAAGGTGGTCTTAGAGCTTTGGAAGCAGGTAATGGAAAACTCAGTCAGTCGGATTATGTGAAAATGTCCGAGTGCTGGGCTTCCAATTTATGCCAGAAAGAGAGAGAGATAATATATGATATTTTTCAGGCATATGAGAAAATGAAGACAAGAAATGGTGAATATGATATTGCTGATTTTGTAATTGATATTCACCGTCGGCTCCAACGTGAGAAATATAAGGGTGATGAAATTAATTTTGTGTACATCGATGAGGTACAGGATCTAACGATGAGTCAAATCATGCTGTTCAGGCATATATGCAATAATGTTGAGGAGGGTTTTGTTTTTTCGGGTGATATGGCCCAAACCATTTCAAAGGGTATTGATTTTAGATTCCAAGATATACGGCATCTGTTCTTCAAGAAGTTTGTCTTGGATTCAAGAAGCAATGAGCATAACCAAAGGAATGACAAAGCAGAAATCTCAAAAATATTTCATTTGTCTCAAAACTTCCTATACACATGGTGGCGTACTGAGAGCATAATTGATCTACTTTGTCATTTTTTCCCTGAATCTATAGATAGTCTGAATCCTGAAACCAGTCCAATACATGGGGAAGCTCCAGTTTTGCTTCAATCTAGAGAACATGAAGATATGATCTTAAAGTTATTTGGGAGTAGTGCTACTAATTTTGTTGGGTTTGGAGCTGAGCAGGTCATCTTGGTGCGTGATGCTACCGTTCAGAAGGAGATTTCTAACTCTTTTGGGAAGCAAGCTCTTGTTCTTACTATTGTGGAGTGCAAGGGGCTCGAGTTCCAGGATGTACTTTTGTACAAGTATTTTGGGTCATCACCCTTAATAAATGAATGGAGGCTGATATATAATTACATGAAGGAACGTGATTTGCTTAGTCCCACTTTACCTAAGTCGATTCCAACATTTGATGGGGCAAAGCACAATTTCTTATGCTTTGAACTGAAGCAATTATATGTTGCTATTTCACGTACAAGAAATAGATTGTGGATTTATGAGGACATGGAAGGTCTCTGGAATCCCATGTTTGACTATTGGATAAAGAAATGTCTTGTACAAGTGAGGCAACTTGATGATTCCCTTGTAGAAAAAATGAAAGTTTCAAGCAGTGCAGAGGAATGGAAATCGCGTGGCATGAAGGCTAAATCCATTCTTAGGGAAGCTGCTGAAATTTTTGAAGATATAGGGATGGCAAATTCTGCTGCCCAGTGCTTTTTTGATTCAGGGGATTATGACGTAGCTGGTAGGATTTATTACCACAAATGTGGTAAACAAGCACGTGAAAGAGCTGCGGAATGCTTCTTTCTAGCAGAGCGCTATGAATCTGCTGCAGAAATATTTCAATCCATAGACAAGGCAGAGTCTGCTGCAAGATGCTATTACGAATTGGGGCAGTATGAAAGGGCAGGGACTATTTATTTGGAAGACTGTGGTGAACCTGGATTGGAAAGAGCTGGGGAGTGTTTCTATCTAGTAGGATGCTATGACCGTGCAGCTGAAGCCTATGCTAGGGGAAATTTTTTATCCGATTGTCTTACTCAACACGCAAGAAAAGAGCATGATCTGGCTATAAGAGGAGAACTGGAGTTTCTTGAGAGCTGTGCATTTCACTATTATGAGGTTGAAGATACAAGATCCAAGATGAAATCTGTTAGAAGTGAAATGTTGTCCAGTCGAAAGATTTTAAATGCTCATCTCTCTTCAAGTCCAAAAATATATTTGTGGGATGACAAACTGATTCGTGATTTTCGTGATCTGAAAAATTATTTGAAAGTCAAACAATCTGAAAAGCAGTTTTCTGTAGATTCACTGGTGTACTTCTGGAATGTTTGGAAGGATATGGTAGTTTACTTGATTGAATATTTTGAAACTGTTGAAACCCAAGCTGTTAATAGAAAGAGAACTTACGGAGACTTCTTTCTGAATTACCTGGAAGTGTGGAGGCAGTTTCATGATGATCTGCGTTTTGTCTACTCTGTACTTACCTCTGATGCAAATTGGGTAAGAGGAATGGACAAAAGGTCTTTTCAAATTAACGGGGAGATTGTCACCATAGATGTTTGGCAGATAATTTCAGCCGCTCATAGCTATTGGACTTCAGAACTTCTTTCAGTTGGCATTAGGATTTTGAATAAGGTTGAAGCGCTTTACAGGGTCAAATCTGATTCAGTTTTCTGCCAAAGCTGGTGTCTTACGCAGATTTATGTTGTTGTGAAGTGTCTCTTGGAATCCAAATATCTGAAGCTAAGGAACGAAGGTTCCTATACACTACAGAATTTTGTTGAACTGTCAACTGAAGATATTGTTGCTTGTATATTTCCTCCTGATTGGAGGAAATCTGTAAGAGAGAACATGATTACTCTTAGACAAAAGGATACTTCTAAAAGTTTGCTGAAACAAGTTGTAGTTGATTTACTTACCAGCTCAAAGAAAAATCTTTCTTATGGCCAAGTTGGAAGGCTGGCAATGATTATTCTTGGGTCTGGTAGTTTTGATGATGATGAACTATACAAGAAACTTATGAAAAATCTGAAATGTAGTCCACCGTGGAAGGTGTTCATTCATAATCTGTGTGGGAGTATCATTGCAAAACAGAAAGGGTCTGTACTTATCAATACCAGTCAAGAAAAGAAAGATGTGTCTCTTGTCCAGGGCCTTCATCAGGCTTTGGCAGAAACTTATTATGCAAACTGGAGGGAGGTTAATGACTATATTTCACCTGATGGTTTCTTTTATCTTATTGAATGCCTTTTGATGTGGGTATCTTGCTTTCAAGGTTTTGTTATCACCACCAAATCATGTTTCATTGAATGGTTGATGCAGAAGGAAGATACCAAACTCACTTCCAGTATACTGGCTGACGTGCTAATATCTTTTGAACCCATCCTTATGTTTCTGATCGCTGTGGTTTCAGGAGGTTCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1237

Amino Acids

143.26

Weight (kDa)

6.02

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 288 - 375 5.3e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
Acc36I ACCTGC 2 cut(s) 788, 1456
AccB7I CCANNNNNTGG 1 cut(s) 1364
AccBSI CCGCTC 1 cut(s) 2867
AccI GTMKAC 2 cut(s) 2757, 3637
AccII CGCG 1 cut(s) 1915
AciI CCGC 3 cut(s) 727, 2078, 2865
AclWI GGATC 3 cut(s) 483, 1041, 2415
AcoI YGGCCR 1 cut(s) 3232
AcuI CTGAAG 5 cut(s) 1751, 2304, 2868, 3047, 3102
AcvI CACGTG 1 cut(s) 2066
AdeI CACNNNGTG 1 cut(s) 1278
AfeI AGCGCT 2 cut(s) 2102, 2931
AfiI CCNNNNNNNGG 5 cut(s) 846, 1364, 1941, 2295, 3448
AflIII ACRYGT 1 cut(s) 370
AhdI GACNNNNNGTC 1 cut(s) 2806
AjiI CACGTC 1 cut(s) 3649
AjnI CCWGG 4 cut(s) 1569, 2224, 2715, 3435
AjuI GAANNNNNNNTTGG 6 cut(s) 1169, 1201, 2993, 3025, 3572, 3604
AleI CACNNNNGTG 1 cut(s) 2049
AloI GAACNNNNNNTCC 4 cut(s) 3028, 3029, 3060, 3061
Alw26I GTCTC 5 cut(s) 1260, 1802, 2691, 3011, 3432
AlwI GGATC 3 cut(s) 483, 1041, 2415
AlwNI CAGNNNCTG 2 cut(s) 200, 2150
Ama87I CYCGRG 1 cut(s) 1562
Aor51HI AGCGCT 2 cut(s) 2102, 2931
AoxI GGCC 5 cut(s) 153, 698, 1113, 3232, 3439
ApeKI GCWGC 7 cut(s) 408, 1949, 1988, 2075, 2114, 2153, 2279
ArsI GACNNNNNNTTYG 4 cut(s) 2188, 2220, 2959, 2991
AseI ATTAAT 1 cut(s) 1005
Asp700I GAANNNNTTC 2 cut(s) 2124, 3341
AspLEI GCGC 2 cut(s) 2103, 2932
AspS9I GGNCC 2 cut(s) 1114, 3439
AvaI CYCGRG 1 cut(s) 1562
BalI TGGCCA 1 cut(s) 3234
BanII GRGCYC 1 cut(s) 1563
BbrPI CACGTG 1 cut(s) 2066
BbsI GAAGAC 3 cut(s) 212, 923, 2217
BbvI GCAGC 7 cut(s) 395, 1936, 1975, 2062, 2101, 2140, 2291
BccI CCATC 5 cut(s) 551, 1691, 1969, 3503, 3677
BceAI ACGGC 1 cut(s) 1175
BcgI CGANNNNNNTGC 2 cut(s) 1543, 1577
BciT130I CCWGG 4 cut(s) 1571, 2226, 2717, 3437
BciVI GTATCC 1 cut(s) 3154
BcoDI GTCTC 5 cut(s) 1260, 1802, 2691, 3011, 3432
BfaI CTAG 4 cut(s) 1392, 2093, 2258, 2295
BfmI CTRYAG 4 cut(s) 1329, 2115, 2586, 3056
BfoI RGCGCY 2 cut(s) 2104, 2933
BfuAI ACCTGC 2 cut(s) 788, 1456
BfuI GTATCC 1 cut(s) 3154
BisI GCNGC 9 cut(s) 409, 728, 1950, 1989, 2076, 2115, 2154, 2280, 2865
BlpI GCTNAGC 1 cut(s) 1461
BlsI GCNGC 9 cut(s) 410, 729, 1951, 1990, 2077, 2116, 2155, 2281, 2866
Bme1390I CCNGG 4 cut(s) 1571, 2226, 2717, 3437
BmeRI GACNNNNNGTC 1 cut(s) 2806
BmeT110I CYCGRG 1 cut(s) 1562
BmgBI CACGTC 1 cut(s) 3649
BmgT120I GGNCC 2 cut(s) 1114, 3439
BmiI GGNNCC 2 cut(s) 974, 3046
BmrFI CCNGG 4 cut(s) 1571, 2226, 2717, 3437
BmrI ACTGGG 2 cut(s) 210, 1987
BmsI GCATC 9 cut(s) 445, 540, 724, 1171, 1474, 2150, 2255, 2767, 3594
BmuI ACTGGG 2 cut(s) 210, 1987
BpiI GAAGAC 3 cut(s) 212, 923, 2217
BpmI CTGGAG 3 cut(s) 1359, 2390, 3499
Bpu10I CCTNAGC 1 cut(s) 3032
Bpu1102I GCTNAGC 1 cut(s) 1461
BpuEI CTTGAG 3 cut(s) 403, 760, 2399
Bsa29I ATCGAT 1 cut(s) 1020
BsaAI YACGTR 2 cut(s) 1757, 2066
BsaI GGTCTC 1 cut(s) 1802
BsaJI CCNNGG 2 cut(s) 3332, 3436
Bsc4I CCNNNNNNNGG 5 cut(s) 846, 1364, 1941, 2295, 3448
Bse3DI GCAATG 3 cut(s) 1207, 3258, 3369
BseBI CCWGG 4 cut(s) 1571, 2226, 2717, 3437
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 2 cut(s) 3332, 3436
BseGI GGATG 7 cut(s) 448, 562, 1579, 1980, 2270, 2518, 3669
BseLI CCNNNNNNNGG 5 cut(s) 846, 1364, 1941, 2295, 3448
BseMI GCAATG 3 cut(s) 1207, 3258, 3369
BseMII CTCAG 3 cut(s) 826, 1278, 1452
BseRI GAGGAG 2 cut(s) 1100, 2376
BseXI GCAGC 7 cut(s) 395, 1936, 1975, 2062, 2101, 2140, 2291
BseYI CCCAGC 2 cut(s) 846, 2240
BsgI GTGCAG 2 cut(s) 1917, 2298
Bsh1236I CGCG 1 cut(s) 1915
BshFI GGCC 5 cut(s) 155, 700, 1115, 3234, 3441
BshVI ATCGAT 1 cut(s) 1020
BsiHKCI CYCGRG 1 cut(s) 1562
BslFI GGGAC 2 cut(s) 1650, 2209
BslI CCNNNNNNNGG 5 cut(s) 846, 1364, 1941, 2295, 3448
BsmAI GTCTC 5 cut(s) 1260, 1802, 2691, 3011, 3432
BsmFI GGGAC 2 cut(s) 1650, 2209
BsmI GAATGC 2 cut(s) 2087, 3536
BsnI GGCC 5 cut(s) 155, 700, 1115, 3234, 3441
Bso31I GGTCTC 1 cut(s) 1802
BsoBI CYCGRG 1 cut(s) 1562
Bsp1286I GDGCHC 1 cut(s) 1563
Bsp1407I TGTACA 3 cut(s) 1014, 1584, 1840
Bsp1720I GCTNAGC 1 cut(s) 1461
BspACI CCGC 3 cut(s) 727, 2078, 2865
BspANI GGCC 5 cut(s) 155, 700, 1115, 3234, 3441
BspCNI CTCAG 3 cut(s) 825, 1279, 1453
BspDI ATCGAT 1 cut(s) 1020
BspFNI CGCG 1 cut(s) 1915
BspHI TCATGA 1 cut(s) 2737
BspLI GGNNCC 2 cut(s) 974, 3046
BspMAI CTGCAG 1 cut(s) 2119
BspMI ACCTGC 2 cut(s) 788, 1456
BspPI GGATC 3 cut(s) 483, 1041, 2415
BspTNI GGTCTC 1 cut(s) 1802
BsrBI CCGCTC 1 cut(s) 2867
BsrDI GCAATG 3 cut(s) 1207, 3258, 3369
BsrGI TGTACA 3 cut(s) 1014, 1584, 1840
BssECI CCNNGG 2 cut(s) 3332, 3436
BssNAI GTATAC 1 cut(s) 3638
Bst1107I GTATAC 1 cut(s) 3638
Bst2UI CCWGG 4 cut(s) 1571, 2226, 2717, 3437
Bst6I CTCTTC 1 cut(s) 2491
BstAPI GCANNNNNTGC 2 cut(s) 1985, 2150
BstAUI TGTACA 3 cut(s) 1014, 1584, 1840
BstBAI YACGTR 2 cut(s) 1757, 2066
BstC8I GCNNGC 2 cut(s) 1527, 3249
BstDEI CTNAG 9 cut(s) 782, 812, 1287, 1461, 1661, 1677, 1940, 3032, 3149
BstDSI CCRYGG 1 cut(s) 3332
BstENI CCTNNNNNAGG 2 cut(s) 2293, 3446
BstF5I GGATG 7 cut(s) 448, 562, 1579, 1980, 2270, 2518, 3669
BstFNI CGCG 1 cut(s) 1915
BstH2I RGCGCY 2 cut(s) 2104, 2933
BstHHI GCGC 2 cut(s) 2103, 2932
BstMAI GTCTC 5 cut(s) 1260, 1802, 2691, 3011, 3432
BstMWI GCNNNNNNNGC 5 cut(s) 794, 1063, 1985, 2150, 2285
BstNI CCWGG 4 cut(s) 1571, 2226, 2717, 3437
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 4 cut(s) 1569, 2224, 2715, 3435
BstSFI CTRYAG 4 cut(s) 1329, 2115, 2586, 3056
BstUI CGCG 1 cut(s) 1915
BstV1I GCAGC 7 cut(s) 395, 1936, 1975, 2062, 2101, 2140, 2291
BstV2I GAAGAC 3 cut(s) 212, 923, 2217
BstX2I RGATCY 3 cut(s) 475, 1033, 2420
BstXI CCANNNNNNTGG 2 cut(s) 2051, 3640
BstYI RGATCY 3 cut(s) 475, 1033, 2420
BstZ17I GTATAC 1 cut(s) 3638
Bsu15I ATCGAT 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 3154
BsuRI GGCC 5 cut(s) 155, 700, 1115, 3234, 3441
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 1 cut(s) 3332
BtrI CACGTC 1 cut(s) 3649
BtsCI GGATG 7 cut(s) 448, 562, 1579, 1980, 2270, 2518, 3669
BtsI GCAGTG 1 cut(s) 1900
BtsIMutI CAGTG 6 cut(s) 37, 198, 210, 1900, 2000, 2594
BveI ACCTGC 2 cut(s) 788, 1456
Cac8I GCNNGC 2 cut(s) 1527, 3249
CaiI CAGNNNCTG 2 cut(s) 200, 2150
CciI TCATGA 1 cut(s) 2737
CfoI GCGC 2 cut(s) 2103, 2932
Cfr13I GGNCC 2 cut(s) 1114, 3439
ClaI ATCGAT 1 cut(s) 1020
DdeI CTNAG 9 cut(s) 782, 812, 1287, 1461, 1661, 1677, 1940, 3032, 3149
DraI TTTAAA 1 cut(s) 2472
DraIII CACNNNGTG 1 cut(s) 1278
DriI GACNNNNNGTC 1 cut(s) 2806
EaeI YGGCCR 1 cut(s) 3232
Eam1104I CTCTTC 1 cut(s) 2491
Eam1105I GACNNNNNGTC 1 cut(s) 2806
EarI CTCTTC 1 cut(s) 2491
Eco24I GRGCYC 1 cut(s) 1563
Eco31I GGTCTC 1 cut(s) 1802
Eco47III AGCGCT 2 cut(s) 2102, 2931
Eco57I CTGAAG 5 cut(s) 1751, 2304, 2868, 3047, 3102
Eco72I CACGTG 1 cut(s) 2066
Eco88I CYCGRG 1 cut(s) 1562
EcoNI CCTNNNNNAGG 2 cut(s) 2293, 3446
EcoO109I RGGNCCY 1 cut(s) 3439
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 4 cut(s) 1569, 2224, 2715, 3435
EcoT38I GRGCYC 1 cut(s) 1563
FalI AAGNNNNNCTT 8 cut(s) 379, 411, 765, 797, 1169, 1201, 2993, 3025
FaqI GGGAC 2 cut(s) 1650, 2209
FauNDI CATATG 1 cut(s) 904
FblI GTMKAC 2 cut(s) 2757, 3637
Fnu4HI GCNGC 9 cut(s) 409, 728, 1950, 1989, 2076, 2115, 2154, 2280, 2865
FokI GGATG 7 cut(s) 455, 569, 1586, 1987, 2277, 2525, 3656
FriOI GRGCYC 1 cut(s) 1563
Fsp4HI GCNGC 9 cut(s) 409, 728, 1950, 1989, 2076, 2115, 2154, 2280, 2865
FspBI CTAG 4 cut(s) 1392, 2093, 2258, 2295
GlaI GCGC 2 cut(s) 2102, 2931
GluI GCNGC 9 cut(s) 409, 728, 1950, 1989, 2076, 2115, 2154, 2280, 2865
GsaI CCCAGC 2 cut(s) 850, 2244
GsuI CTGGAG 3 cut(s) 1359, 2390, 3499
HaeII RGCGCY 2 cut(s) 2104, 2933
HaeIII GGCC 5 cut(s) 155, 700, 1115, 3234, 3441
HhaI GCGC 2 cut(s) 2103, 2932
Hin6I GCGC 2 cut(s) 2101, 2930
HinP1I GCGC 2 cut(s) 2101, 2930
HincII GTYRAC 2 cut(s) 720, 3078
HindII GTYRAC 2 cut(s) 720, 3078
HindIII AAGCTT 1 cut(s) 646
HpaI GTTAAC 1 cut(s) 720
Hpy99I CGWCG 1 cut(s) 972
HpyCH4IV ACGT 6 cut(s) 979, 1651, 1756, 2022, 2065, 3648
HpyF10VI GCNNNNNNNGC 5 cut(s) 794, 1063, 1985, 2150, 2285
HpyF3I CTNAG 9 cut(s) 782, 812, 1287, 1461, 1661, 1677, 1940, 3032, 3149
HpySE526I ACGT 6 cut(s) 979, 1651, 1756, 2022, 2065, 3648
HspAI GCGC 2 cut(s) 2101, 2930
KspAI GTTAAC 1 cut(s) 720
LmnI GCTCC 3 cut(s) 978, 1378, 1457
Lsp1109I GCAGC 7 cut(s) 395, 1936, 1975, 2062, 2101, 2140, 2291
LweI GCATC 9 cut(s) 445, 540, 724, 1171, 1474, 2150, 2255, 2767, 3594
MaeI CTAG 4 cut(s) 1392, 2093, 2258, 2295
MaeII ACGT 6 cut(s) 979, 1651, 1756, 2022, 2065, 3648
MaeIII GTNAC 3 cut(s) 133, 331, 2833
MbiI CCGCTC 1 cut(s) 2867
MflI RGATCY 3 cut(s) 475, 1033, 2420
MhlI GDGCHC 1 cut(s) 1563
MlsI TGGCCA 1 cut(s) 3234
MluNI TGGCCA 1 cut(s) 3234
MlyI GAGTC 3 cut(s) 753, 1054, 2156
MmeI TCCRAC 4 cut(s) 801, 1000, 1712, 3220
Mox20I TGGCCA 1 cut(s) 3234
MroXI GAANNNNTTC 2 cut(s) 2124, 3341
MscI TGGCCA 1 cut(s) 3234
MslI CAYNNNNRTG 4 cut(s) 1475, 2049, 2400, 3674
Msp20I TGGCCA 1 cut(s) 3234
MspA1I CMGCKG 2 cut(s) 419, 2282
MspR9I CCNGG 4 cut(s) 1571, 2226, 2717, 3437
Mva1269I GAATGC 2 cut(s) 2087, 3536
MvaI CCWGG 4 cut(s) 1571, 2226, 2717, 3437
MvnI CGCG 1 cut(s) 1915
MwoI GCNNNNNNNGC 5 cut(s) 794, 1063, 1985, 2150, 2285
NdeI CATATG 1 cut(s) 904
NlaIV GGNNCC 2 cut(s) 974, 3046
NmuCI GTSAC 1 cut(s) 2833
NspI RCATGY 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 2049
PaeR7I CTCGAG 1 cut(s) 1562
PagI TCATGA 1 cut(s) 2737
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 976, 2533
PctI GAATGC 2 cut(s) 2087, 3536
PdmI GAANNNNTTC 2 cut(s) 2124, 3341
PflMI CCANNNNNTGG 1 cut(s) 1364
PfoI TCCNGGA 1 cut(s) 1569
PkrI GCNGC 9 cut(s) 410, 729, 1951, 1990, 2077, 2116, 2155, 2281, 2866
PleI GAGTC 3 cut(s) 752, 1053, 2155
PmaCI CACGTG 1 cut(s) 2066
PmlI CACGTG 1 cut(s) 2066
PpsI GAGTC 3 cut(s) 752, 1053, 2155
Ppu21I YACGTR 2 cut(s) 1757, 2066
PscI ACATGT 1 cut(s) 370
PshBI ATTAAT 1 cut(s) 1005
PsiI TTATAA 1 cut(s) 527
Psp6I CCWGG 4 cut(s) 1569, 2224, 2715, 3435
PspCI CACGTG 1 cut(s) 2066
PspFI CCCAGC 2 cut(s) 846, 2240
PspGI CCWGG 4 cut(s) 1569, 2224, 2715, 3435
PspN4I GGNNCC 2 cut(s) 974, 3046
PspPI GGNCC 2 cut(s) 1114, 3439
PspXI VCTCGAGB 1 cut(s) 1562
PstI CTGCAG 1 cut(s) 2119
PstNI CAGNNNCTG 2 cut(s) 200, 2150
PsuI RGATCY 3 cut(s) 475, 1033, 2420
PvuII CAGCTG 2 cut(s) 419, 2282
RseI CAYNNNNRTG 4 cut(s) 1475, 2049, 2400, 3674
SatI GCNGC 9 cut(s) 409, 728, 1950, 1989, 2076, 2115, 2154, 2280, 2865
Sau96I GGNCC 2 cut(s) 1114, 3439
SchI GAGTC 3 cut(s) 753, 1054, 2156
ScrFI CCNGG 4 cut(s) 1571, 2226, 2717, 3437
SduI GDGCHC 1 cut(s) 1563
SfaNI GCATC 9 cut(s) 445, 540, 724, 1171, 1474, 2150, 2255, 2767, 3594
SfcI CTRYAG 4 cut(s) 1329, 2115, 2586, 3056
Sfr274I CTCGAG 1 cut(s) 1562
SlaI CTCGAG 1 cut(s) 1562
SmiMI CAYNNNNRTG 4 cut(s) 1475, 2049, 2400, 3674
SmlI CTYRAG 4 cut(s) 382, 739, 1562, 2378
SmoI CTYRAG 4 cut(s) 382, 739, 1562, 2378
SsiI CCGC 3 cut(s) 727, 2078, 2865
SspI AATATT 3 cut(s) 1245, 2124, 2648
SspMI CTAG 4 cut(s) 1392, 2093, 2258, 2295
StyD4I CCNGG 4 cut(s) 1569, 2224, 2715, 3435
TaiI ACGT 6 cut(s) 982, 1654, 1759, 2025, 2068, 3651
TaqI TCGA 8 cut(s) 282, 377, 431, 613, 1020, 1563, 1682, 2461
TatI WGTACW 7 cut(s) 1014, 1576, 1584, 1840, 2601, 2764, 3391
TauI GCSGC 2 cut(s) 730, 2867
TscAI CASTG 6 cut(s) 37, 205, 217, 1900, 2000, 2601
TseFI GTSAC 1 cut(s) 2833
TseI GCWGC 7 cut(s) 408, 1949, 1988, 2075, 2114, 2153, 2279
Tsp45I GTSAC 1 cut(s) 2833
TspGWI ACGGA 2 cut(s) 767, 2709
TspRI CASTG 6 cut(s) 37, 205, 217, 1900, 2000, 2601
Van91I CCANNNNNTGG 1 cut(s) 1364
VspI ATTAAT 1 cut(s) 1005
XagI CCTNNNNNAGG 2 cut(s) 2293, 3446
XbaI TCTAGA 1 cut(s) 1391
XceI RCATGY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 1562
XmiI GTMKAC 2 cut(s) 2757, 3637
XmnI GAANNNNTTC 2 cut(s) 2124, 3341
XspI CTAG 4 cut(s) 1392, 2093, 2258, 2295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.