Rroxscaffold_5G00386440

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000223
Physical Location & Seq
Forward (+)
65352250 .. 65373557
21308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386440.1

Sequence Viewer

Length: 7743 bp
ATGGAGGTTTCAGGCAAGAAAAAGAGCAAGCCTGACCCTTTCACTGATACTGTGTTTTCTTGGTCCCTGGAGGACATTTTCAATGAGGATCTTTACAAAGACAAGGTGAAAAAGATTCCTGAATCATTTCCATCGGCTCAGCATTACTTTGGGTCTTATATATATCCTTTACTAGAAGAAACCCGAGCAGAATTGCATTCAAGTATGGAAACTCTTAATAGAGCACCGTTTGCTGAAGTAGTGGGAACCAAGCCATATGGAACAAAGCAATATGATGTCAAGGTTGATTGCTGGCGAAACAGGTTCATTGATAGTGGCAAGGAGCCATATAAAACTTTGCCCGGTGATCTTTTTGTTTTAGCTGATGCTAAACCTGAAACTGTTTCTGATTTACAAAGAGTGTCATGGGCTTTTGCTTCGGCCACTTATGTCCCAGAAAGTGAGAATGAAGATAACAGTAATGAAGGTGACAGTACCTCTCTTTATTTCAAAGTCAAGGCATCAAAAGAGATTGAAGTCGAAAATAGCAGGACATCACTTTTTATAGTTTTCTTGGTGAGTTTAGTCCGTGATGGAAGAATTTGGAAAGCGTTGCACATGTCCAGAAATCTGAAGATTATCAACGAAGTTCTATGCACTGAATCTATGGTAATGAAGGTTCAGAAAGATTTCTGCTCTGAAAGGAATAATGACATCGGGAATCAGAGTATGATAAACTCTCAACTATCTGGATTGAATGAATCCCAAACTGAGGCAGTGTTGGCCTGCCTTGAAATGCTGCATTCTGGTGAAAAGTCTGCCGTGGAACTTATCTGGGGTCCTCCTGGTACTGGAAAAACAAAAACCACAGTTACTCTGCTTTCAACCCTGCTACAGATGAATTGTAGGACTCTTATTTGTGCTCCAACAAATGTGGCAATAACTGGAGTTGCTTCTCGTCTTGTGAAGATGGTGAGTAGAGCAGAGCCAAATGATTTGTATTGTTCTTTAGGAGAAATTCTTCTGTTTGGGAATAAGGAGCGACTCAAAATTGGTTCAGACATTGAAGAAATTTATATGGATTGCCGTGTCAAAAGGCTTGAATATTGTTTGGGGCCAAGGACTGGTTGGAGGCATTGCTTTGCTTCCATGATAGGTTTACTTGAGGATTGTGTTTCTGATTACCATATTTTCTTGGAAAATGAGTTCACGAAAGAGAGAGAACAAATGAATGTTAGTGAAACGAAAGATAAAGAATGCAGAAGAGGTGCTCAAGGAAAAATTATATTTCGGGTAGACAATTTCCAAGACTTGGTTTCGCTTATCCGGTTAGTTGATTCCTTTGAGTTACTGTTGTCTCATGATAATGTTGATTCTGAAGCTTTGGAAGAACTCTTTTCTCCTTCGGATGCTGAAGATGTGTCCGAGTCGTTTGTAGGTAACTCCTTTGATCTTTGTATGATGAGAAGGGATTGCCTTTCAGCTTTACGCACTCTACAGGATTCTCTTAGTCAACTTAAACTTCCAAATATTATGAATGAGGAATCTATACGGAAGTTCTGTTTTCAAAGAGCATCTCTAATATTTTGCACTGCATCTAGTTCGTATAAGCTGCATAGAGTGGCAATGGAACCACTAAGCATTGTTGTTATTGACGAGGCTGCACAGCTAAAAGAGTGTGAGTCAACAATACCCCTGCAACTTCCAGGTGTGAAGCATGCTGTTCTTGTTGGTGATGAATGTCAGTTACCAGCTACGGTAATAAGCAAGGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTGAGCCTAATGGGTCATTCAAAACATATTTTAAATATGCAATACAGAATGCATCCATCAATAAGTTTATTCCCAAATTCAAATTTCTATAAAGACTTGATCCGGGATGCTCCAAATGTAAAACAAAAAAGCTATGAGAAGCACTATCTTCCAGGAGCAATGTTTGGTCCTTATTCTTTTATAAATGTGATTGATGGAAGAGAGGAGAAAGAAGAGGATGGACCCAGTCGGAAAAATATGGTGGAGGTTGCTATTGTTTCAAAAATACTGCGGAATCTGTACAAAGGCATGTCTAGCAGGAGATTCTTCTTCATATTCTCATCTCAACTCAGTATTGGTATAGTGTCTCCCTATGCTGCTCAAGTAGTCGCAATTAAGGACAGAGTGGGTAAGAAATATGATAAGCTTGATGGCTTTGAAGTGAAGGTAAAAACAGTTGATGGGTTTCAAGGTGGGGAAGAGGACATAATTATAATGTCCACCGTACGATCCAACAGCCATCAATCACTTGAATTCATTTCAAAATCACAAAGAGTTAATGTCTCTATTACAAGGGCGAGGCACTGTTTGTGGATTTTGGGGAATGAAAGAACTCTTTCCTATAGTGAATCTGTTTGGAATGACTTGGTCCTTGATGCCAAAAGTCGTCGATGCTTCTTTAATGCCGATCAAGACAAGGATTTAGCCAAGGCCATATTAGGGGTAAAGAAAGAGTTTGAGCAATTTGATGATTTGCTCAATGCCAACAGCATACTTTTTAGAAGTGCTAGATGGAAGGTACTTTTCAGTGATAACTTTCTGAAGTCATTCAAGAAACTGACTTCAGTCCGCTTGAAGAAGTTGGTGCTACATCTTCTACTGAAGCTTTCCAGTGGTTGGAAACCTAAGAAGCGAAATGTTGAGACAATTTCTGGTAGCTCTTCCATGATCTTAAAGAAATTTAAGGTTGAAGGTCTGTACATTGTTTGCACTAATGACATTTCAAAGAACCTGAAATACGTTCAAGTATTAAAGATCTGGGACATATTGCCTCTAGAGGAGATCCCAAAGTTGATAAACCGCCTGGATAGTATTTTCAACAGATGTACTGATAACTTTATTAATCTTTGCAAGGAGAAGTGTCTTGATGGTGATCTAGAGGTTCCAAAAAGCTGGTCACTGTCTTTGGAATTTTCCCGGATTAAGGATCTTAGCATCAGTGAAGCTCAGAGTGATTTAGTTGGTGACACTTCTGATTGTAGAAGCTATGTTGAGAATTCACAGGTTAGCGAGAGTTTGTTGCTGTTAAAATTTTATTCCTTATCTTCTGGGGTAGTAAACCACTTGCTGTCCGACCGTGAGGGTAGAGAACTGGAGCTTCCATTTGAAGTTACAGACGAAGAGATGGAGATTATCCTTTATCGTAGAAGTTCCTTTATAGTGGGACGGTCAGGAACTGGGAAAACCACAGTTTTAACCATGAAATTGTTTCAAAAAGAACAGTGGCACCAATTTGCAGTTCAAGGATGCTATAGTAGTCAAAATACTAGCAAGGTTGAGCAGAGTTCTGCAGCTACTGAAGGGAAAGTTCTGCGCCAGCTTTTTGTGACATTGAGTCCTAAGCTATGTTTTGCCATCAAGCGACATGTTTCACACTTGAAAAGTTTGATTTTGGTCGATAAAGATTGGAAAGAAATAAAAACCAACACTGGTACAACTACTGTTGGCCATTTTAGGAGTTCGAAATCAGTTGCATTTCAGAGCTCTATAAGGACGAAAGAAGTTAATTATGAGAGGTTTAGCTCTTTCTATTGGCCCCGTTTTAATACTCAGATAACAAAGAAGCTTGACGTTTCAAGAGTCTTTATAGAGATTATTTCTTACATAAAAGGTGGCTTAGGAGCTATAGATGCATGTGGCGGTAAACTCAGCCGGGAGGATTATGTTCAACTGTGTGAGGGCCGGGGTTCTAATTTGAGCAAGCAAAAGAGAGAAGAAATATACGATGTTTTTCAGGCTTATGAAAAGTTGAAGATGGAAAATGGTGAATTTGATCTGGCTGATTTTGTAATTGATCTTCACTGTCGGCTCAAGCATGACAAATATAAGGGTGATCTGATGGAATTTGTTTATATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTTAAACACATGTGCAACAATGTTGAACAGGGATTCGTCTTTTCTGGTGATACAGCACAAACAATAGCAAGGGGTATTGACTTTAGGTTTCAGGATATACGACATCTGTTCTACAAGAAGTTTGTATTGGAGTCAAGAAACAATAACAACGATGAAAGAAAAGAAAAGGGCCTCATCTCTGAATTACTTCAGTTGACTCAAAACTTCCGTACTCATGCTGGTATATTGAAGTTATCACAAAGCATTGTTGAACTACTGTATCGGTTTTTCCCCTTTTCTGTTGATGTTTTAAAACCTGAAACCAGTCTGATTTATGGGGAAGCTCCAGTTTTGCTTGAATCTGGGGAAAACGAAAATGCAATCATGAAAATTTTTGGTAACAGTGGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATTTTGGTGCGAGATGATGGTGCTCGACGAGAAATATCCAAATTTGTAGGGAAGCATGCTCTTGTCCTAACCATAGTGGAGTGTAAGGGCCTTGAATTTCAGGATGTCCTCTTATACAACTTTTTTGGCACATCACCTTTGAAAAATCAGTGGAGGGTTATATATGATTACATGAAGGAACAAGATTTACTCGACTCCACATTACCTACGCGCTTTCCAAGTTTCAGTGAATCAAAACACAACATATTGTGCTCTGAACTGAAGCAATTATATGTGGCTGTTACTCGAACAAGACAGAGATTGTGGATTTGTGAAAATGTGGAAGACCTCTCCAAACCTATGTTTGACTATTGGAAGAAAAAATGTCTTGTGCAACAAAGGCAGCTAGATGATTCACTTACCCAGGCAATGCAAGTGGCAAGCAGTCCAGAGGAGTGGAAATCCCGAGGCATTAAGCTATATCATGAACATAACTATGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGACACTTACTGGGAAAGGAGGTCTAAAGCTTCCGGCCTTAAAGCCATTGCTGACCGTATGCGAACATCAAATCCCGAAGAGGCTAAATCGATCCTTAGAAAAGCTGCTGAAATATTTGATGCTATTGGCAAGGCAGATTCTGCTGCGAGATGTTTTTCTGATTTGGGGGAGTATGAAACAGCAGCTAGGATATATTTGGAAAAATGTGGAGACTCTGAACTTCAAAGAGCGGCAGAATGTTTTTCACTAGCGGGATGCTACAAGGATGCTGCAGATGTCTATGCCAAGGGAAATTTTTTCTCAGAGTGTCTCACTGTTTGTGCAAATGGGAAACTATTTGAAATGGGTTTGCAGTATATTAATTATTGGAAACAGCGTGCTGTTGAGGACTGTGCTAAGGCTAGTAGAGGGGAAGGAATAAATAAAAAGGAGCAGGAATTTTTAGAGGGCTGCGCACTTCACTATCATAAGTTGAAAGATAACAGATTAATGATGAAATTTGTGAAAGCTTTCAATTCTATAATATTGATGCGGGACTTTTTGAAGAGTATAGGGAGCCTTGATGAGCTTTTGTCATTGGAGGAAGAATTTGGAAATTATCTGGAAGCCGCAAACATCGCCCAGCTTAAAGGTGAAATCCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAGGCCTCACTGCATATTCTGTTTTATGTAATTTCCAAGTCACTTTGGTCATCTGGTGGCAAGGGCTGGCCCATGGAAAAGTTTCCACAACAGGGGGAGCTTCTGACAAAGGCCAAGTCATTTGCCATGAATGGGAAAGATAACGTTTATGAGCTGGTTTGCGCTGAGTCGGAGATTTTATTGAATGGGCAGGATAAGTTGGCTGTTTTGAAACATCAAATCGATGCTTCTGAAAGACATCAGAGTATTAGAGGTGAAGTGTTATCAGCTCGAAAAATTTTGGACGCCCATTTTTCTTCAAGAACTGATGAGTATTTATGGGAGAAAGAATTGATTGGAGATCATGTAAAGCATTCAAAAGATATAATATCTAGAAATCAGGTGTCCATTGATTCGCTTGTCTACTACTGGAATTTCTGGAAGGAGAAGATCCTTCACATCATTGAATCTGTAGGATGTGTGGAAACTCCAGGGTACAATAGTTATGTAGAACTCTGTTTCACTTGTTTAGGAGTTTGGAGGTTGTATCATAATCTGAATCCAATCTATGTTTTACTGGTCCCTGATGCTGATTGGGTTAGACATCTGGACATAAGGCATTTGAAAAGTTATGGGAAGTTGGTCTCCATTGATGTTCATCAGCTCATTTCAGCAGCTCAGTATTATTGGAGTTCAGAACTGCTCTCCGTTGGCATCAAAGTTTTGGAGAAGCTTGAAGCACTTTTCAAGTTCCCACTGAAGAATTCTGACTCGACATTCTTCCAGTGCAGGGCTCTTACCCTTATCTATGATGTTGCAAGAAATCTTTTGGATTCCAAATGTCTGAAGCCAAGGTATCAAGATTACTTGACACTACAGAATATGGTAACATTTTCGACTGGGGAGTATGTTGATTACATCTTTCCCTTAGATTGGAGGAATTCATTGAGACAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATATGCCAGCTTGAAGGACGAGCTGTCTTATGGGCAGATTGGAAGGATCGCAATGATCATTCTCGGGTCAGGTAAGCTGCTGAATACTGAGCTCTCTGTTCAGCTTGTGAAAAAGCTTGAAATCAATAAACCATGGAGGGCCTTCATTGAGAATCTCTGTGCAAGCATTGGACCTGAACATACCCATCAGGAACCAATAGAGGTGTCTGTCATGTGGTGGTTACATGAGGCTTTGGTTGGAACTTATAGTGCCAACTGGAGGGAGGTTTCCGACTACATAACACCTGGTTGCTTCTTGTATCTTGTGGAGCGCCTTCTGTTTTCCGCAAGTTGCTTTCAAGGTTTTGTCATCACTACAAGTTCTTGCTTTATTGAATGGCTGATATACCAGGATGAAGATACCAGCTTACGTTCCATGGTGACTGGTGGGGGCCATTCTCTGGAAGACATTCTTCCATTTGTGATTACTGTGGTTCGCGAGTGTATTTTTATCAAGAAGGATATGATTGAATGGATTGAGAAGTCCGACAGAAATTGGATGCAGTATTATCAGCCACTCATGTTGCGATTGGTAATTGTCTTGTGTTTGATTCATTTGAACTTTGGCTCGTGTTTGGATATGATCCATGATTTGCTGGGAAGAAACTTTATCACTGAACTCCTACCACGGGAGTTTTATGATGCACTTAGGAGGAGGAGGAGGAGAAGGAAACACAATTCTCTCATTTTAAATGTGAATGTGCTTGCTGAAGCTCTCCGGAAGATTGATAATACTTTGGTGATTGCAAGTTTCGGGGCTGATTGTTCAAGGTTGTACTGTTCGGATGCCATCTTTGTCGACATGACGGCCAACCAATGCAAGGATGACATACTGAGAAAATGGTTTCCAAATCCGACATATCAACAAGATGTTGATACTTCCAACCCATCTGGAAGCAGGCGAAGAACAAGACAAAGGGAAAGAACAAGAAAAACAAGAAGAAACGAGTGGGATGTCATGGTGCAAGATCCAGAGTACATTGAATATTCTGGTGGTCTCGGTGGTGATCAGTGGCCTACCCAAATTGCTGATGCTTCCTGGTTCTGCAGTCCAGCCGGTGTGACACAAGACGGTTGGGATGCTGCTCCTGCACCAGTGGTCGCCGCTCCTATCCTTGAGGGTGGTGCTCCCGTAGTGGCCAGAGGTTGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2580

Amino Acids

295.14

Weight (kDa)

6.51

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 76 - 204 2.9e-52 Domain of unknown function (DUF6469)
AAA_11 PF13086 245 - 583 4.1e-36 AAA domain
AAA_19 PF13245 265 - 578 7.1e-07 AAA domain
AAA_12 PF13087 591 - 793 4.1e-54 AAA domain
UvrD-helicase PF00580 1248 - 1337 1.5e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1973, 2264
AarI CACCTGC 1 cut(s) 4348
Acc16I TGCGCA 1 cut(s) 5326
Acc36I ACCTGC 2 cut(s) 4348, 5531
AccB1I GGYRCC 1 cut(s) 3276
AccB7I CCANNNNNTGG 5 cut(s) 1103, 1291, 2667, 6988, 7734
AccBSI CCGCTC 2 cut(s) 5102, 7694
AccI GTMKAC 3 cut(s) 1275, 5955, 7386
AccII CGCG 2 cut(s) 4573, 7026
AccIII TCCGGA 1 cut(s) 7305
AclI AACGTT 1 cut(s) 5697
AcoI YGGCCR 5 cut(s) 420, 3496, 4845, 7395, 7725
AcyI GRCGYC 1 cut(s) 5838
AdeI CACNNNGTG 1 cut(s) 4854
AflIII ACRYGT 3 cut(s) 597, 3415, 3954
AhdI GACNNNNNGTC 2 cut(s) 3384, 6571
AjuI GAANNNNNNNTTGG 4 cut(s) 3273, 3305, 4055, 4087
AloI GAACNNNNNNTCC 4 cut(s) 1169, 1201, 4927, 4959
Alw21I GWGCWC 8 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6642, 7717
Alw26I GTCTC 9 cut(s) 1341, 2142, 2338, 2687, 5076, 5186, 6181, 6475, 7589
AlwNI CAGNNNCTG 3 cut(s) 3227, 3347, 5012
Ama87I CYCGRG 3 cut(s) 183, 4806, 6611
Aor13HI TCCGGA 1 cut(s) 7305
ArsI GACNNNNNNTTYG 4 cut(s) 2930, 2962, 5654, 5686
AseI ATTAAT 3 cut(s) 2892, 5232, 5360
Asp700I GAANNNNTTC 9 cut(s) 126, 668, 999, 2386, 2597, 4131, 5381, 5634, 6996
AspLEI GCGC 5 cut(s) 3366, 4575, 5327, 5717, 6861
AsuC2I CCSGG 5 cut(s) 342, 1895, 2968, 3704, 3734
AsuII TTCGAA 1 cut(s) 3512
AvaI CYCGRG 3 cut(s) 183, 4806, 6611
AvaII GGWCC 7 cut(s) 63, 818, 1958, 2012, 2419, 6112, 6719
BaeI ACNNNNGTAYC 4 cut(s) 4187, 4220, 6019, 6052
BalI TGGCCA 3 cut(s) 3498, 4847, 7727
BanI GGYRCC 1 cut(s) 3276
BanII GRGCYC 3 cut(s) 3536, 6328, 6642
BarI GAAGNNNNNNTAC 2 cut(s) 3831, 3863
BauI CACGAG 1 cut(s) 7156
BbsI GAAGAC 2 cut(s) 4692, 6999
Bbv12I GWGCWC 8 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6642, 7717
BceAI ACGGC 3 cut(s) 785, 1050, 7410
BcgI CGANNNNNNTGC 2 cut(s) 1563, 1597
BclI TGATCA 2 cut(s) 6603, 7594
BcnI CCSGG 5 cut(s) 342, 1895, 2968, 3704, 3734
BcoDI GTCTC 9 cut(s) 1341, 2142, 2338, 2687, 5076, 5186, 6181, 6475, 7589
BfoI RGCGCY 1 cut(s) 6862
BfuAI ACCTGC 2 cut(s) 4348, 5531
BglII AGATCT 2 cut(s) 2805, 3919
BlpI GCTNAGC 1 cut(s) 138
Bme18I GGWCC 7 cut(s) 63, 818, 1958, 2012, 2419, 6112, 6719
BmeRI GACNNNNNGTC 2 cut(s) 3384, 6571
BmeT110I CYCGRG 3 cut(s) 183, 4806, 6611
BmrI ACTGGG 4 cut(s) 2010, 3237, 4891, 6441
BmuI ACTGGG 4 cut(s) 2010, 3237, 4891, 6441
BoxI GACNNNNGTC 1 cut(s) 2615
BpiI GAAGAC 2 cut(s) 4692, 6999
BpmI CTGGAG 6 cut(s) 89, 945, 3164, 4254, 6006, 6826
Bpu10I CCTNAGC 3 cut(s) 3390, 3667, 5268
Bpu1102I GCTNAGC 1 cut(s) 138
Bpu14I TTCGAA 1 cut(s) 3512
BpuEI CTTGAG 5 cut(s) 1163, 1236, 2136, 3845, 7724
BpuMI CCSGG 5 cut(s) 342, 1895, 2968, 3704, 3734
Bsa29I ATCGAT 2 cut(s) 4961, 5775
BsaBI GATNNNNATC 2 cut(s) 2922, 6189
BsaHI GRCGYC 1 cut(s) 5838
BsaI GGTCTC 2 cut(s) 6181, 7589
BsaWI WCCGGW 2 cut(s) 1305, 7305
BsaXI ACNNNNNCTCC 2 cut(s) 5883, 5913
Bse118I RCCGGY 1 cut(s) 7643
Bse3DI GCAATG 6 cut(s) 1114, 1611, 1956, 4776, 4917, 6606
Bse8I GATNNNNATC 2 cut(s) 2922, 6189
BseAI TCCGGA 1 cut(s) 7305
BseCI ATCGAT 2 cut(s) 4961, 5775
BseJI GATNNNNATC 2 cut(s) 2922, 6189
BseMI GCAATG 6 cut(s) 1114, 1611, 1956, 4776, 4917, 6606
BseRI GAGGAG 7 cut(s) 2009, 2843, 4808, 7255, 7258, 7261, 7264
BseYI CCCAGC 2 cut(s) 5493, 7183
BsgI GTGCAG 4 cut(s) 1626, 4371, 6340, 7662
Bsh1236I CGCG 2 cut(s) 4573, 7026
Bsh1285I CGRYCG 1 cut(s) 3127
BshNI GGYRCC 1 cut(s) 3276
BshVI ATCGAT 2 cut(s) 4961, 5775
BsiEI CGRYCG 1 cut(s) 3127
BsiHKAI GWGCWC 8 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6642, 7717
BsiHKCI CYCGRG 3 cut(s) 183, 4806, 6611
BsiSI CCGG 9 cut(s) 342, 1306, 1894, 2968, 3703, 3733, 4905, 7306, 7644
BsiWI CGTACG 1 cut(s) 2275
BslFI GGGAC 6 cut(s) 49, 416, 2825, 3228, 5420, 6098
BsmAI GTCTC 9 cut(s) 1341, 2142, 2338, 2687, 5076, 5186, 6181, 6475, 7589
BsmFI GGGAC 6 cut(s) 49, 416, 2825, 3228, 5420, 6098
BsmI GAATGC 5 cut(s) 196, 781, 1241, 1845, 5905
Bso31I GGTCTC 2 cut(s) 6181, 7589
BsoBI CYCGRG 3 cut(s) 183, 4806, 6611
Bsp119I TTCGAA 1 cut(s) 3512
Bsp1286I GDGCHC 9 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6328, 6642, 7717
Bsp13I TCCGGA 1 cut(s) 7305
Bsp1407I TGTACA 2 cut(s) 2070, 2748
Bsp1720I GCTNAGC 1 cut(s) 138
Bsp19I CCATGG 3 cut(s) 5625, 6680, 6963
Bsp68I TCGCGA 1 cut(s) 7026
BspDI ATCGAT 2 cut(s) 4961, 5775
BspEI TCCGGA 1 cut(s) 7305
BspFNI CGCG 2 cut(s) 4573, 7026
BspHI TCATGA 3 cut(s) 1339, 4308, 4825
BspMAI CTGCAG 3 cut(s) 3343, 5146, 7637
BspMI ACCTGC 2 cut(s) 4348, 5531
BspQI GCTCTTC 1 cut(s) 2716
BspT104I TTCGAA 1 cut(s) 3512
BspT107I GGYRCC 1 cut(s) 3276
BspTNI GGTCTC 2 cut(s) 6181, 7589
BsrBI CCGCTC 2 cut(s) 5102, 7694
BsrDI GCAATG 6 cut(s) 1114, 1611, 1956, 4776, 4917, 6606
BsrFI RCCGGY 1 cut(s) 7643
BsrGI TGTACA 2 cut(s) 2070, 2748
BssAI RCCGGY 1 cut(s) 7643
BssNI GRCGYC 1 cut(s) 5838
BssSI CACGAG 1 cut(s) 7156
BssT1I CCWWGG 8 cut(s) 1097, 2478, 5157, 5530, 5625, 6383, 6680, 6963
Bst2BI CACGAG 1 cut(s) 7156
Bst6I CTCTTC 8 cut(s) 1237, 1984, 1998, 2244, 2716, 3165, 4944, 5411
BstACI GRCGYC 1 cut(s) 5838
BstAPI GCANNNNNTGC 2 cut(s) 230, 5012
BstAUI TGTACA 2 cut(s) 2070, 2748
BstBI TTCGAA 1 cut(s) 3512
BstDSI CCRYGG 5 cut(s) 801, 5625, 6680, 6963, 7214
BstENI CCTNNNNNAGG 1 cut(s) 5534
BstFNI CGCG 2 cut(s) 4573, 7026
BstH2I RGCGCY 1 cut(s) 6862
BstHHI GCGC 5 cut(s) 3366, 4575, 5327, 5717, 6861
BstMAI GTCTC 9 cut(s) 1341, 2142, 2338, 2687, 5076, 5186, 6181, 6475, 7589
BstMCI CGRYCG 1 cut(s) 3127
BstMWI GCNNNNNNNGC 9 cut(s) 230, 761, 1769, 2085, 3680, 4741, 5012, 5489, 7676
BstNSI RCATGY 7 cut(s) 601, 1700, 2083, 3419, 3687, 3958, 4421
BstPAI GACNNNNGTC 1 cut(s) 2615
BstUI CGCG 2 cut(s) 4573, 7026
BstV2I GAAGAC 2 cut(s) 4692, 6999
BstX2I RGATCY 7 cut(s) 88, 2805, 2832, 2977, 3919, 5982, 7555
BstXI CCANNNNNNTGG 2 cut(s) 2943, 4797
BstYI RGATCY 7 cut(s) 88, 2805, 2832, 2977, 3919, 5982, 7555
Bsu15I ATCGAT 2 cut(s) 4961, 5775
BsuTUI ATCGAT 2 cut(s) 4961, 5775
BtgI CCRYGG 5 cut(s) 801, 5625, 6680, 6963, 7214
BtgZI GCGATG 1 cut(s) 5473
BtsI GCAGTG 3 cut(s) 762, 1569, 5561
BtuMI TCGCGA 1 cut(s) 7026
BveI ACCTGC 2 cut(s) 4348, 5531
CaiI CAGNNNCTG 3 cut(s) 3227, 3347, 5012
CciI TCATGA 3 cut(s) 1339, 4308, 4825
CfoI GCGC 5 cut(s) 3366, 4575, 5327, 5717, 6861
Cfr10I RCCGGY 1 cut(s) 7643
ClaI ATCGAT 2 cut(s) 4961, 5775
CseI GACGC 1 cut(s) 5846
CsiI ACCWGGT 1 cut(s) 6832
CspCI CAANNNNNGTGG 6 cut(s) 894, 929, 4758, 4793, 7092, 7127
DraI TTTAAA 4 cut(s) 1824, 3949, 4236, 7278
DraIII CACNNNGTG 1 cut(s) 4854
DriI GACNNNNNGTC 2 cut(s) 3384, 6571
EaeI YGGCCR 5 cut(s) 420, 3496, 4845, 7395, 7725
Eam1104I CTCTTC 8 cut(s) 1237, 1984, 1998, 2244, 2716, 3165, 4944, 5411
Eam1105I GACNNNNNGTC 2 cut(s) 3384, 6571
EarI CTCTTC 8 cut(s) 1237, 1984, 1998, 2244, 2716, 3165, 4944, 5411
Ecl136II GAGCTC 2 cut(s) 3534, 6640
Eco130I CCWWGG 8 cut(s) 1097, 2478, 5157, 5530, 5625, 6383, 6680, 6963
Eco147I AGGCCT 1 cut(s) 5558
Eco24I GRGCYC 3 cut(s) 3536, 6328, 6642
Eco31I GGTCTC 2 cut(s) 6181, 7589
Eco47I GGWCC 7 cut(s) 63, 818, 1958, 2012, 2419, 6112, 6719
Eco53kI GAGCTC 2 cut(s) 3534, 6640
Eco88I CYCGRG 3 cut(s) 183, 4806, 6611
EcoICRI GAGCTC 2 cut(s) 3534, 6640
EcoNI CCTNNNNNAGG 1 cut(s) 5534
EcoO109I RGGNCCY 4 cut(s) 818, 4114, 4450, 6687
EcoRI GAATTC 4 cut(s) 2303, 3046, 6295, 6472
EcoT14I CCWWGG 8 cut(s) 1097, 2478, 5157, 5530, 5625, 6383, 6680, 6963
EcoT22I ATGCAT 2 cut(s) 1845, 3685
EcoT38I GRGCYC 3 cut(s) 3536, 6328, 6642
ErhI CCWWGG 8 cut(s) 1097, 2478, 5157, 5530, 5625, 6383, 6680, 6963
FalI AAGNNNNNCTT 2 cut(s) 6984, 7016
FaqI GGGAC 6 cut(s) 49, 416, 2825, 3228, 5420, 6098
FauI CCCGC 2 cut(s) 5116, 5397
FauNDI CATATG 1 cut(s) 256
FbaI TGATCA 2 cut(s) 6603, 7594
FblI GTMKAC 3 cut(s) 1275, 5955, 7386
FriOI GRGCYC 3 cut(s) 3536, 6328, 6642
FspI TGCGCA 1 cut(s) 5326
GlaI GCGC 5 cut(s) 3365, 4574, 5326, 5716, 6860
GsaI CCCAGC 2 cut(s) 5497, 7187
GsuI CTGGAG 6 cut(s) 89, 945, 3164, 4254, 6006, 6826
HaeII RGCGCY 1 cut(s) 6862
HapII CCGG 9 cut(s) 342, 1306, 1894, 2968, 3703, 3733, 4905, 7306, 7644
HgaI GACGC 1 cut(s) 5846
HhaI GCGC 5 cut(s) 3366, 4575, 5327, 5717, 6861
Hin1I GRCGYC 1 cut(s) 5838
Hin6I GCGC 5 cut(s) 3364, 4573, 5325, 5715, 6859
HinP1I GCGC 5 cut(s) 3364, 4573, 5325, 5715, 6859
HincII GTYRAC 4 cut(s) 1493, 1665, 4140, 7387
HindII GTYRAC 4 cut(s) 1493, 1665, 4140, 7387
HindIII AAGCTT 9 cut(s) 1359, 1777, 2195, 2654, 3614, 4899, 5379, 6263, 6662
HpaII CCGG 9 cut(s) 342, 1306, 1894, 2968, 3703, 3733, 4905, 7306, 7644
Hpy99I CGWCG 2 cut(s) 2442, 4392
HpyCH4IV ACGT 4 cut(s) 2790, 3621, 5697, 6958
HpyF10VI GCNNNNNNNGC 9 cut(s) 230, 761, 1769, 2085, 3680, 4741, 5012, 5489, 7676
HpySE526I ACGT 4 cut(s) 2790, 3621, 5697, 6958
Hsp92I GRCGYC 1 cut(s) 5838
HspAI GCGC 5 cut(s) 3364, 4573, 5325, 5715, 6859
Kpn2I TCCGGA 1 cut(s) 7305
Ksp22I TGATCA 2 cut(s) 6603, 7594
LguI GCTCTTC 1 cut(s) 2716
MabI ACCWGGT 1 cut(s) 6832
MaeII ACGT 4 cut(s) 2790, 3621, 5697, 6958
MbiI CCGCTC 2 cut(s) 5102, 7694
MflI RGATCY 7 cut(s) 88, 2805, 2832, 2977, 3919, 5982, 7555
MhlI GDGCHC 9 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6328, 6642, 7717
MlsI TGGCCA 3 cut(s) 3498, 4847, 7727
MluNI TGGCCA 3 cut(s) 3498, 4847, 7727
Mox20I TGGCCA 3 cut(s) 3498, 4847, 7727
Mph1103I ATGCAT 2 cut(s) 1845, 3685
MroI TCCGGA 1 cut(s) 7305
MroXI GAANNNNTTC 9 cut(s) 126, 668, 999, 2386, 2597, 4131, 5381, 5634, 6996
MscI TGGCCA 3 cut(s) 3498, 4847, 7727
MslI CAYNNNNRTG 3 cut(s) 786, 1344, 4836
Msp20I TGGCCA 3 cut(s) 3498, 4847, 7727
MspI CCGG 9 cut(s) 342, 1306, 1894, 2968, 3703, 3733, 4905, 7306, 7644
MssI GTTTAAAC 1 cut(s) 3949
Mva1269I GAATGC 5 cut(s) 196, 781, 1241, 1845, 5905
MvnI CGCG 2 cut(s) 4573, 7026
MwoI GCNNNNNNNGC 9 cut(s) 230, 761, 1769, 2085, 3680, 4741, 5012, 5489, 7676
NciI CCSGG 5 cut(s) 342, 1895, 2968, 3704, 3734
NcoI CCATGG 3 cut(s) 5625, 6680, 6963
NdeI CATATG 1 cut(s) 256
NmuCI GTSAC 8 cut(s) 467, 2946, 3014, 3376, 4871, 5592, 6967, 7648
NruI TCGCGA 1 cut(s) 7026
NsbI TGCGCA 1 cut(s) 5326
NsiI ATGCAT 2 cut(s) 1845, 3685
NspI RCATGY 7 cut(s) 601, 1700, 2083, 3419, 3687, 3958, 4421
NspV TTCGAA 1 cut(s) 3512
PaeI GCATGC 2 cut(s) 1700, 4421
PagI TCATGA 3 cut(s) 1339, 4308, 4825
PaqCI CACCTGC 1 cut(s) 4348
PceI AGGCCT 1 cut(s) 5558
PciI ACATGT 3 cut(s) 597, 3415, 3954
PciSI GCTCTTC 1 cut(s) 2716
PctI GAATGC 5 cut(s) 196, 781, 1241, 1845, 5905
PdmI GAANNNNTTC 9 cut(s) 126, 668, 999, 2386, 2597, 4131, 5381, 5634, 6996
Pfl23II CGTACG 1 cut(s) 2275
PflFI GACNNNGTC 2 cut(s) 2016, 2417
PflMI CCANNNNNTGG 5 cut(s) 1103, 1291, 2667, 6988, 7734
PfoI TCCNGGA 3 cut(s) 1893, 1942, 2966
PmeI GTTTAAAC 1 cut(s) 3949
PpuMI RGGWCCY 1 cut(s) 818
PscI ACATGT 3 cut(s) 597, 3415, 3954
PshAI GACNNNNGTC 1 cut(s) 2615
PshBI ATTAAT 3 cut(s) 2892, 5232, 5360
PsiI TTATAA 2 cut(s) 1973, 2264
Psp124BI GAGCTC 2 cut(s) 3536, 6642
Psp1406I AACGTT 1 cut(s) 5697
Psp5II RGGWCCY 1 cut(s) 818
PspFI CCCAGC 2 cut(s) 5493, 7183
PspLI CGTACG 1 cut(s) 2275
PspPPI RGGWCCY 1 cut(s) 818
PsrI GAACNNNNNNTAC 6 cut(s) 642, 674, 2771, 2803, 4533, 4565
PstI CTGCAG 3 cut(s) 3343, 5146, 7637
PstNI CAGNNNCTG 3 cut(s) 3227, 3347, 5012
PsuI RGATCY 7 cut(s) 88, 2805, 2832, 2977, 3919, 5982, 7555
PsyI GACNNNGTC 2 cut(s) 2016, 2417
RruI TCGCGA 1 cut(s) 7026
RseI CAYNNNNRTG 3 cut(s) 786, 1344, 4836
SacI GAGCTC 2 cut(s) 3536, 6642
SalI GTCGAC 1 cut(s) 7385
SapI GCTCTTC 1 cut(s) 2716
SduI GDGCHC 9 cut(s) 226, 904, 1252, 3536, 4387, 4616, 6328, 6642, 7717
SexAI ACCWGGT 1 cut(s) 6832
SfuI TTCGAA 1 cut(s) 3512
SinI GGWCC 7 cut(s) 63, 818, 1958, 2012, 2419, 6112, 6719
SmiMI CAYNNNNRTG 3 cut(s) 786, 1344, 4836
SmlI CTYRAG 5 cut(s) 1142, 1251, 2151, 3860, 7703
SmoI CTYRAG 5 cut(s) 1142, 1251, 2151, 3860, 7703
SphI GCATGC 2 cut(s) 1700, 4421
SseBI AGGCCT 1 cut(s) 5558
SspI AATATT 6 cut(s) 1085, 1510, 1563, 4986, 5397, 7574
SstI GAGCTC 2 cut(s) 3536, 6642
StuI AGGCCT 1 cut(s) 5558
StyI CCWWGG 8 cut(s) 1097, 2478, 5157, 5530, 5625, 6383, 6680, 6963
TaiI ACGT 4 cut(s) 2793, 3624, 5700, 6961
TatI WGTACW 5 cut(s) 2070, 2748, 2876, 7362, 7563
TauI GCSGC 3 cut(s) 5105, 5483, 7694
TseFI GTSAC 8 cut(s) 467, 2946, 3014, 3376, 4871, 5592, 6967, 7648
Tsp45I GTSAC 8 cut(s) 467, 2946, 3014, 3376, 4871, 5592, 6967, 7648
TspGWI ACGGA 4 cut(s) 557, 1546, 4142, 6229
Tth111I GACNNNGTC 2 cut(s) 2016, 2417
Van91I CCANNNNNTGG 5 cut(s) 1103, 1291, 2667, 6988, 7734
VpaK11BI GGWCC 7 cut(s) 63, 818, 1958, 2012, 2419, 6112, 6719
VspI ATTAAT 3 cut(s) 2892, 5232, 5360
XagI CCTNNNNNAGG 1 cut(s) 5534
XbaI TCTAGA 3 cut(s) 2824, 2926, 5924
XceI RCATGY 7 cut(s) 601, 1700, 2083, 3419, 3687, 3958, 4421
XcmI CCANNNNNNNNNTGG 1 cut(s) 1104
XmiI GTMKAC 3 cut(s) 1275, 5955, 7386
XmnI GAANNNNTTC 9 cut(s) 126, 668, 999, 2386, 2597, 4131, 5381, 5634, 6996
Zsp2I ATGCAT 2 cut(s) 1845, 3685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.