RchiOBHm_Chr4g0445971

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000223
Physical Location & Seq
Forward (+)
66284234 .. 66290888
6655 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41351

Sequence Viewer

Length: 4632 bp
ATGAAGTATTTTGAGGGTGACTTGGAAGTTCCAATGAGCTGGCCAGCCTCCTTGGATGTAATCCGATATAAGGATCTTAGCGTCACTAGTGATTCAGTGGATGACGTGTCTAAGGATAAAAGTTATGTTGAAAATTCAAAGGTCAGTGAGAGTTTTTTGCTGATGAAATTCTACTCCTTATCATCTGGGGTGGTGAATCACTTGCTCTCTGACCGTGAGGGTAGAGAGTTGGTTCTCCCATTTGAAGTTACTGATGAAGAAATGGAGGCCATACTTTACAATAGAAGTGCCTTCATAGTTGGGCGATCAGGCACTGGGAAGACCACTGTTTTAACAATGAAGTTATATCAGAAGGAACAGTATTACAATATGGCAAAGGAAGGATTGTATGCTGTCGTGCAGAGTTCTGGAGGTTCTAATGCAACTGTTTTACGCCAGATATTTGTAACAGTTAGTCCAAAACTCTGTTTTGCCATAAAGCAACATGTATCGAACTTGAGAAGTTTTGCTTCTGGTGTAAGCTGCCATTCAGCTGAATTCGGCTCGATTGGTCTGGATGATTATGATGATGCAGAAGCAGAATTTAGGGATCTCCCAAATTCATTTCTTGATATTCCTTCCGAGCGTTATCCTCTTATTATAACATTCCATAAATTTTTAATGATGCTGGATGGAAGTTTGAGTAATTCATACTTTGAAAGATTCCTTGACAACCTAAAACTGCCTCGAAGGCCACCGCAAAGTTCAAGATCGCTGAAAAGCTTATTGAGGACAAAGGAGGTCAATTACGAAAGGTTTTGTTCATCATATTGGCCTCATTTTAATATGAAGTTAACGAAGCGGCTTGATGCCTTGAGAGTCATTACTGAGATTATTTCTCATATCAAAGGTGGTCTTAGAGCTTTGGAAGCAGGTAATGGAAAACTCAGTCAGTCGGATTATGTGAAAATGTCTGAGTGCCGGGCTTCCAATTTATGCCAGAAAGAGAGAGAGATAATATATGATATTTTTCAGGTATATGAGAAAATGAAGACAAGAAATGGTGAATATGATATTGCTGATTTTGTAATTGATATTCACCGTCGACTCCAACGTGAGAAATATAAGGGTGATGAAATTAATTTTGTGTACATCGATGAGGTACAGGATCTAACGATGAGTCAAATCATGCTGTTCAAGCATATATGCAGTAATGTTGAGGAGGGTTTTGTTTTTTCGGGTGATACGGCCCAAACCATTTCAAAGGGTATTGATTTTAGATTCCAAGATATACGGCATCTGTTCTACAAGAAGTTTGTCTTGGATTCAAGAAGCAATGAGCATAACGAAAGGAATGACAAAGCAGAAATCTCAAAAATGTTTCATTTGTCTCAAAACTTCCGCACACATGGTGGCGTCATCTTGGTGCGTGATGCTACCGTTCAGAAGGAGATTTCTAACTCTTTTGGGAAGCAAGCTCTTGTTCTTACTATTGTGGAGTGCAAGGGGCTCGAGTTCCAGGATGTACTTTTGTACAAGTATTTTGGGTCATCACCCTTAATAAATGAATGGAGGCTGATATATAATTACATGAAGGAACATGATTTGCTTAGTCCCACTTTACATAAGTCGATTCCAACATTTGACAGGGCAAAACACAATTTTTTATGCTCTGAACTGAAGCAATTATATGTTGCTATTTCACGTACAAGAAATAGATTGTGGATTTATGAGGACATGGAAGGTCTCTGGAATCCCATGTTTGACTATTGGATAAAGAAATGTCTTGTACAAGTGAGGCAACTTGATGATTCCCTTGTAGAAAAAATGAAAGTTTCAAGCAGTGCAGAGGAATGGAAATCGCGTGGCATGAAGCTGTATCATGAGCGTAATTATGAAATGGCCAATATGTGCTTCCAAAATGCACATGATACTTACTGGGAAAGAAGGTCAAAAGCCGGCAGTCTCAAAGTTATGGCTTATTGTATGCAAACATCAAATCCTGAAAAGGCTAAATCCATTCTTAGGGAAGCTGCTGAAATTTTTGAAGATATAGGGATGGCAAATTCTGCTGCCCAATGCTTTTTTGATTCAGGGGATTATGATGTAGCAGGTAGGATTTATTACCACAAATGTGGTAAACAAGCACGTGAAAGAGCTGCGGAATGCTTCTTTCTAGCAGAGCGCTATGAATCTGCTGCAGAAATATTTCAATCCATAGGCATGGCAGAGTCTGCTGCAAGATGCTATTACGAATTGGGGCAGTATGAAAGGGCAGGGACTATTTATTTGGAAGACTGTGGTGACCCTGGACTGGAAAGAGCTGGGGAGTGTTTCTATCTAGCAGGATGCTATAAGGGTGCAGCTGATGCCTATGGTAGGGGAAATTTTTTATCCGATTGTCTTAGTGTGTGTTTAAAGGGAAAGCTGTTTGACATAGGTTTGAAATATATTAAAAGTTGGAAGCAACACGCAAGAAAAGAGCATGATCTGGCTATAAGAGGAGAACTGGAGTTTCTTGAGAGCTGTGCATTTCACTATTATGAGGTTGAAGATACAAGATCCAACATGAAATCTGTTAGAAGTGAAATGTTGTCCAGTCGAAAGATTTTAAATGCTCATCTCTCTTCTAGTCCAAAAATATATTTGTGGGATGACAAACTGATTCGTGATTTTCGTGATCTGAAAATCTATTTGAAAGTCAAACAATCTGAAAAGCAGTTTTCTGTAGATTCACTGGTGTACTTCTGGAATGTTTGGAAGGATATGGTAGTTTACTTGATTGAATATTTTGAATCTGTTGAAACCCAAGCTGTTAATAGAAAGAGAACTTACGGAGACTTCTTTCTGAATTACCTGGGAGTGTGGAGGCAGTTTCATGATGATCTGTGTTTTGTCTACTCTGTACTGATCTCTGATGCAAATTGGGTAAGAGGAATGGACAAAAGGTCTTTTCAAATTAACGGGGAGCTGGTCACCATAGATGTTTGGCAGATAATTTCCGCCGCTCATAGCTATTGGACTTCAGAACTTCTTTCAGTTGGCATTAGGATTTTGAATAAGGTTGAAGCGCTTTACAGGGTCAAATCTGATTCAGTTTTCTGCCAAAGCTGGTGTCTTACGCAGATTTATGTTGTTGCGAAGTGTCTCTTGGAATCCAAATATCTGAAGCTAAGGAACGAAGATTCCTATACACTACAGAATTTTGTTGAACTGTCATCTGAAGATATTGTTGCTTGTATATTTCCTCCTGATTGGAGGAAATCTGTAAGAGAGAACATGATTACTCATAGACAAAAGGATACTTCTAAAAGTTTGCTCAAACAAGTTGTAGTTGATTTATTTACCAGCTCAAAGAAAAATCTTTCTTATGGCCAAGTTGGAAGGCTGGCAATGATTATTCTTGGGTCTGGTAATTTTGATGATGATGAACTATACAAGAAACTTATGAAAAATCTGAAATGTAGTCCACTGTGGAAGGTGTTCATTCAGAATCTGTGTGGGAGTATCATCGCAAAACAGAAAGGGTATGTACTTATCAATACCAGTCAAGAAAAGAAAGATGTGTCTCTTGTCCAGGGCCTTCATCAGGCTTTGGCAGAAACTTATTATGCAAACTGGAGGGAGGTTAATGACTATATTTCACCTGATGGTTTCTTTTATCTTATTGAATGCCTTTTGATGTGGGTATCTTGCTTTCAAGGTTTTGTTATCACCACCAAATCATGTTTCATTGAATGGTTGATGCAGAAGGAAGATACCAAACTCACTGCCAGTGTACTGGCTGATGTGCTAATATCTTTTGAACCCATCCTTATGTTTCTGATCGATGTGGTTCGGGAGGTTCTTTATGATAAGAAGACTATGGTTGAATGGATTCAAAGGTCTATCTCACAGTGGGAGGAGTGTCATTCGCTGCTGATCCTGAGATCGGTTATTGGTAATGATTTGGTGGTTGCAAGTTTTGGGGTTGATTGTTCAAGGTTCTTTTGTCCAGATTCCATTTCTGTAGAGATGAAGACCAACCTATGCAGTAATGAGATACTCAGAAAATTGTTTCAAAGACCACATATTCATGATCAATATTCCCAAGGTCAAACTATTATCGGGGAAGTTTGCAGTCCTGTTCCTTCTGCTTATGATTCGGAGGAGACCAAGGTTATTGGGAGTCCTAGGCTTCTTCTACCCAACTCTGGTGTGGTGGAAGATGAGGTCCAACACACTGGAAACAGTAACAAAAGTGACCTTCCAATGGGTTTTGATGCTTTGTGGGACAAATTTAAAAGTTTTAAGTTGGTGGAGAAGGATGGTCAGAGGAGTTTACTTTCAGATGCCTCAGCATTTAAGTTGTTTGTGGGGAAAATTATTTTCCTTTTGACTGCTGTCTGGTTGGCAAGCTTTCCAAATGCTGTTGACATAGAAGAACAAATAGTATCTCGAGAAGTTCTGAGCATGCTTGATGAGTTGATGCAACTTTATGCGGCCTTACATGGCAGTGAAAAGGAACTTGGGAACAAAATGTCTACCGTTGCAAAACTTTCTGCGAAGTTGCTAGTGAGGAGGCAAAGAATGGAGCCTATGTTTTTTTGGCCAGAGAAAAGGGAGGCCTATCTACAAGATGCGGCTGCTACTGGAACACAAACCGGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1543

Amino Acids

178.05

Weight (kDa)

6.29

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 324 - 413 2.8e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 641
Acc36I ACCTGC 2 cut(s) 902, 2081
AccBSI CCGCTC 1 cut(s) 2987
AccI GTMKAC 3 cut(s) 1084, 2877, 4502
AccII CGCG 1 cut(s) 1843
AciI CCGC 8 cut(s) 737, 841, 1381, 2141, 2982, 2985, 4460, 4601
AclWI GGATC 5 cut(s) 81, 597, 1155, 2535, 3895
AcoI YGGCCR 4 cut(s) 41, 1881, 3352, 4568
AcuI CTGAAG 4 cut(s) 1679, 2988, 3167, 3222
AcvI CACGTG 1 cut(s) 2129
AcyI GRCGYC 1 cut(s) 1395
AdeI CACNNNGTG 1 cut(s) 1391
AfeI AGCGCT 2 cut(s) 2165, 3051
AfiI CCNNNNNNNGG 8 cut(s) 70, 2004, 2293, 2358, 3568, 3910, 4172, 4231
AflIII ACRYGT 2 cut(s) 105, 484
AhdI GACNNNNNGTC 1 cut(s) 2926
AhlI ACTAGT 1 cut(s) 86
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 4 cut(s) 1497, 2287, 2835, 3555
AleI CACNNNNGTG 1 cut(s) 2112
AloI GAACNNNNNNTCC 2 cut(s) 3149, 3181
Alw26I GTCTC 7 cut(s) 1374, 1730, 1949, 2811, 3131, 3552, 4124
AlwI GGATC 5 cut(s) 81, 597, 1155, 2535, 3895
AlwNI CAGNNNCTG 3 cut(s) 314, 2213, 3475
Ama87I CYCGRG 2 cut(s) 1490, 4416
Aor51HI AGCGCT 2 cut(s) 2165, 3051
ArsI GACNNNNNNTTYG 4 cut(s) 2251, 2283, 3079, 3111
AseI ATTAAT 1 cut(s) 1119
Asp700I GAANNNNTTC 3 cut(s) 2187, 3461, 3855
AspA2I CCTAGG 1 cut(s) 4151
AspLEI GCGC 2 cut(s) 2166, 3052
AspS9I GGNCC 3 cut(s) 1228, 3559, 4192
AsuC2I CCSGG 1 cut(s) 962
AvaI CYCGRG 2 cut(s) 1490, 4416
AvaII GGWCC 1 cut(s) 4192
AvrII CCTAGG 1 cut(s) 4151
BalI TGGCCA 4 cut(s) 43, 1883, 3354, 4570
BanII GRGCYC 1 cut(s) 1491
BbrPI CACGTG 1 cut(s) 2129
BbsI GAAGAC 5 cut(s) 326, 1037, 2280, 3845, 4004
BbvCI CCTCAGC 1 cut(s) 4315
BbvI GCAGC 9 cut(s) 509, 1999, 2038, 2125, 2164, 2203, 2354, 3882, 4591
BccI CCATC 5 cut(s) 665, 2032, 3623, 3797, 4280
BceAI ACGGC 2 cut(s) 1242, 1289
BcgI CGANNNNNNTGC 2 cut(s) 1471, 1505
BciT130I CCWGG 4 cut(s) 1499, 2289, 2837, 3557
BciVI GTATCC 1 cut(s) 3274
BclI TGATCA 1 cut(s) 4057
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 7 cut(s) 1374, 1730, 1949, 2811, 3131, 3552, 4124
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 7 cut(s) 87, 2156, 2321, 2610, 4152, 4532, 4630
BfmI CTRYAG 4 cut(s) 2178, 2706, 3176, 3987
BfoI RGCGCY 2 cut(s) 2167, 3053
BfuAI ACCTGC 2 cut(s) 902, 2081
BfuI GTATCC 1 cut(s) 3274
BglI GCCNNNNNGGC 1 cut(s) 730
BlnI CCTAGG 1 cut(s) 4151
Bme1390I CCNGG 5 cut(s) 962, 1499, 2289, 2837, 3557
Bme18I GGWCC 1 cut(s) 4192
BmeRI GACNNNNNGTC 1 cut(s) 2926
BmeT110I CYCGRG 2 cut(s) 1490, 4416
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 1228, 3559, 4192
BmiI GGNNCC 1 cut(s) 4554
BmrFI CCNGG 5 cut(s) 962, 1499, 2289, 2837, 3557
BmrI ACTGGG 2 cut(s) 324, 1927
BmuI ACTGGG 2 cut(s) 324, 1927
BoxI GACNNNNGTC 1 cut(s) 4361
BpiI GAAGAC 5 cut(s) 326, 1037, 2280, 3845, 4004
BpmI CTGGAG 3 cut(s) 429, 2510, 3619
Bpu10I CCTNAGC 2 cut(s) 3152, 4315
BpuEI CTTGAG 3 cut(s) 517, 874, 2519
BpuMI CCSGG 1 cut(s) 962
Bsa29I ATCGAT 2 cut(s) 1134, 3807
BsaAI YACGTR 2 cut(s) 1685, 2129
BsaHI GRCGYC 1 cut(s) 1395
BsaI GGTCTC 2 cut(s) 1730, 4124
BsaJI CCNNGG 7 cut(s) 51, 2287, 2836, 3556, 4069, 4134, 4151
BsaXI ACNNNNNCTCC 2 cut(s) 4544, 4574
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 2004, 2293, 2358, 3568, 3910, 4172, 4231
Bse118I RCCGGY 2 cut(s) 1937, 4622
Bse3DI GCAATG 2 cut(s) 1321, 3378
BseBI CCWGG 4 cut(s) 1499, 2289, 2837, 3557
BseCI ATCGAT 2 cut(s) 1134, 3807
BseDI CCNNGG 7 cut(s) 51, 2287, 2836, 3556, 4069, 4134, 4151
BseLI CCNNNNNNNGG 8 cut(s) 70, 2004, 2293, 2358, 3568, 3910, 4172, 4231
BseMI GCAATG 2 cut(s) 1321, 3378
BseMII CTCAG 7 cut(s) 858, 940, 945, 3896, 4039, 4329, 4418
BseRI GAGGAG 6 cut(s) 1214, 2496, 3896, 4142, 4309, 4552
BseXI GCAGC 9 cut(s) 509, 1999, 2038, 2125, 2164, 2203, 2354, 3882, 4591
BseYI CCCAGC 1 cut(s) 2303
BsgI GTGCAG 3 cut(s) 419, 1845, 2361
Bsh1236I CGCG 1 cut(s) 1843
BshVI ATCGAT 2 cut(s) 1134, 3807
BsiHKCI CYCGRG 2 cut(s) 1490, 4416
BsiSI CCGG 3 cut(s) 961, 1938, 4623
BslFI GGGAC 3 cut(s) 1578, 2272, 4265
BslI CCNNNNNNNGG 8 cut(s) 70, 2004, 2293, 2358, 3568, 3910, 4172, 4231
BsmAI GTCTC 7 cut(s) 1374, 1730, 1949, 2811, 3131, 3552, 4124
BsmFI GGGAC 3 cut(s) 1578, 2272, 4265
BsmI GAATGC 2 cut(s) 2150, 3656
Bso31I GGTCTC 2 cut(s) 1730, 4124
BsoBI CYCGRG 2 cut(s) 1490, 4416
Bsp1286I GDGCHC 1 cut(s) 1491
Bsp1407I TGTACA 3 cut(s) 1128, 1512, 1768
BspACI CCGC 8 cut(s) 737, 841, 1381, 2141, 2982, 2985, 4460, 4601
BspCNI CTCAG 7 cut(s) 859, 939, 946, 3897, 4038, 4328, 4419
BspDI ATCGAT 2 cut(s) 1134, 3807
BspFNI CGCG 1 cut(s) 1843
BspHI TCATGA 3 cut(s) 1861, 2857, 4054
BspLI GGNNCC 1 cut(s) 4554
BspMAI CTGCAG 1 cut(s) 2182
BspMI ACCTGC 2 cut(s) 902, 2081
BspPI GGATC 5 cut(s) 81, 597, 1155, 2535, 3895
BspTNI GGTCTC 2 cut(s) 1730, 4124
BsrBI CCGCTC 1 cut(s) 2987
BsrDI GCAATG 2 cut(s) 1321, 3378
BsrFI RCCGGY 2 cut(s) 1937, 4622
BsrGI TGTACA 3 cut(s) 1128, 1512, 1768
BssAI RCCGGY 2 cut(s) 1937, 4622
BssECI CCNNGG 7 cut(s) 51, 2287, 2836, 3556, 4069, 4134, 4151
BssNI GRCGYC 1 cut(s) 1395
BssT1I CCWWGG 4 cut(s) 51, 4069, 4134, 4151
Bst2UI CCWGG 4 cut(s) 1499, 2289, 2837, 3557
Bst6I CTCTTC 1 cut(s) 2611
BstACI GRCGYC 1 cut(s) 1395
BstAPI GCANNNNNTGC 3 cut(s) 2048, 2213, 2348
BstAUI TGTACA 3 cut(s) 1128, 1512, 1768
BstBAI YACGTR 2 cut(s) 1685, 2129
BstC8I GCNNGC 7 cut(s) 41, 45, 1455, 1939, 3369, 4375, 4433
BstEII GGTNACC 2 cut(s) 2282, 2953
BstENI CCTNNNNNAGG 2 cut(s) 2356, 3566
BstFNI CGCG 1 cut(s) 1843
BstH2I RGCGCY 2 cut(s) 2167, 3053
BstHHI GCGC 2 cut(s) 2166, 3052
BstMAI GTCTC 7 cut(s) 1374, 1730, 1949, 2811, 3131, 3552, 4124
BstMWI GCNNNNNNNGC 6 cut(s) 730, 908, 1177, 2048, 2213, 2348
BstNI CCWGG 4 cut(s) 1499, 2289, 2837, 3557
BstNSI RCATGY 2 cut(s) 488, 4435
BstPAI GACNNNNGTC 1 cut(s) 4361
BstPI GGTNACC 2 cut(s) 2282, 2953
BstSCI CCNGG 5 cut(s) 960, 1497, 2287, 2835, 3555
BstSFI CTRYAG 4 cut(s) 2178, 2706, 3176, 3987
BstUI CGCG 1 cut(s) 1843
BstV1I GCAGC 9 cut(s) 509, 1999, 2038, 2125, 2164, 2203, 2354, 3882, 4591
BstV2I GAAGAC 5 cut(s) 326, 1037, 2280, 3845, 4004
BstX2I RGATCY 4 cut(s) 73, 589, 1147, 2540
BstXI CCANNNNNNTGG 5 cut(s) 39, 2114, 2203, 3760, 4202
BstYI RGATCY 4 cut(s) 73, 589, 1147, 2540
Bsu15I ATCGAT 2 cut(s) 1134, 3807
BsuI GTATCC 1 cut(s) 3274
BsuTUI ATCGAT 2 cut(s) 1134, 3807
BtgZI GCGATG 1 cut(s) 3475
BtrI CACGTC 1 cut(s) 106
BtsI GCAGTG 3 cut(s) 1828, 3747, 4480
BveI ACCTGC 2 cut(s) 902, 2081
Cac8I GCNNGC 7 cut(s) 41, 45, 1455, 1939, 3369, 4375, 4433
CaiI CAGNNNCTG 3 cut(s) 314, 2213, 3475
CciI TCATGA 3 cut(s) 1861, 2857, 4054
CfoI GCGC 2 cut(s) 2166, 3052
Cfr10I RCCGGY 2 cut(s) 1937, 4622
Cfr13I GGNCC 3 cut(s) 1228, 3559, 4192
ClaI ATCGAT 2 cut(s) 1134, 3807
CseI GACGC 2 cut(s) 70, 1384
DraI TTTAAA 3 cut(s) 2397, 2592, 4261
DraIII CACNNNGTG 1 cut(s) 1391
DriI GACNNNNNGTC 1 cut(s) 2926
EaeI YGGCCR 4 cut(s) 41, 1881, 3352, 4568
Eam1104I CTCTTC 1 cut(s) 2611
Eam1105I GACNNNNNGTC 1 cut(s) 2926
EarI CTCTTC 1 cut(s) 2611
EciI GGCGGA 1 cut(s) 2971
Eco130I CCWWGG 4 cut(s) 51, 4069, 4134, 4151
Eco147I AGGCCT 1 cut(s) 4586
Eco24I GRGCYC 1 cut(s) 1491
Eco31I GGTCTC 2 cut(s) 1730, 4124
Eco47I GGWCC 1 cut(s) 4192
Eco47III AGCGCT 2 cut(s) 2165, 3051
Eco57I CTGAAG 4 cut(s) 1679, 2988, 3167, 3222
Eco72I CACGTG 1 cut(s) 2129
Eco88I CYCGRG 2 cut(s) 1490, 4416
Eco91I GGTNACC 2 cut(s) 2282, 2953
EcoNI CCTNNNNNAGG 2 cut(s) 2356, 3566
EcoO109I RGGNCCY 1 cut(s) 3559
EcoO65I GGTNACC 2 cut(s) 2282, 2953
EcoRI GAATTC 1 cut(s) 536
EcoRII CCWGG 4 cut(s) 1497, 2287, 2835, 3555
EcoT14I CCWWGG 4 cut(s) 51, 4069, 4134, 4151
EcoT38I GRGCYC 1 cut(s) 1491
ErhI CCWWGG 4 cut(s) 51, 4069, 4134, 4151
FalI AAGNNNNNCTT 8 cut(s) 493, 525, 879, 911, 1283, 1315, 3113, 3145
FaqI GGGAC 3 cut(s) 1578, 2272, 4265
FbaI TGATCA 1 cut(s) 4057
FblI GTMKAC 3 cut(s) 1084, 2877, 4502
FriOI GRGCYC 1 cut(s) 1491
FspBI CTAG 7 cut(s) 87, 2156, 2321, 2610, 4152, 4532, 4630
GlaI GCGC 2 cut(s) 2165, 3051
GsaI CCCAGC 1 cut(s) 2307
GsuI CTGGAG 3 cut(s) 429, 2510, 3619
HaeII RGCGCY 2 cut(s) 2167, 3053
HapII CCGG 3 cut(s) 961, 1938, 4623
HgaI GACGC 2 cut(s) 70, 1384
HhaI GCGC 2 cut(s) 2166, 3052
Hin1I GRCGYC 1 cut(s) 1395
Hin6I GCGC 2 cut(s) 2164, 3050
HinP1I GCGC 2 cut(s) 2164, 3050
HincII GTYRAC 3 cut(s) 834, 1085, 4393
HindII GTYRAC 3 cut(s) 834, 1085, 4393
HindIII AAGCTT 2 cut(s) 760, 4375
HpaI GTTAAC 1 cut(s) 834
HpaII CCGG 3 cut(s) 961, 1938, 4623
Hpy99I CGWCG 1 cut(s) 1086
HpyCH4IV ACGT 4 cut(s) 105, 1093, 1684, 2128
HpyF10VI GCNNNNNNNGC 6 cut(s) 730, 908, 1177, 2048, 2213, 2348
HpySE526I ACGT 4 cut(s) 105, 1093, 1684, 2128
Hsp92I GRCGYC 1 cut(s) 1395
HspAI GCGC 2 cut(s) 2164, 3050
KroI GCCGGC 1 cut(s) 1937
KroNI GCCGGC 1 cut(s) 1939
Ksp22I TGATCA 1 cut(s) 4057
KspAI GTTAAC 1 cut(s) 834
LmnI GCTCC 2 cut(s) 2947, 4552
Lsp1109I GCAGC 9 cut(s) 509, 1999, 2038, 2125, 2164, 2203, 2354, 3882, 4591
MaeI CTAG 7 cut(s) 87, 2156, 2321, 2610, 4152, 4532, 4630
MaeII ACGT 4 cut(s) 105, 1093, 1684, 2128
MaeIII GTNAC 8 cut(s) 17, 82, 247, 445, 2282, 2953, 4211, 4220
MbiI CCGCTC 1 cut(s) 2987
MflI RGATCY 4 cut(s) 73, 589, 1147, 2540
MhlI GDGCHC 1 cut(s) 1491
MlsI TGGCCA 4 cut(s) 43, 1883, 3354, 4570
MluNI TGGCCA 4 cut(s) 43, 1883, 3354, 4570
MlyI GAGTC 5 cut(s) 867, 1080, 1168, 2219, 4156
MmeI TCCRAC 7 cut(s) 915, 1114, 1640, 2420, 2568, 3340, 4219
Mox20I TGGCCA 4 cut(s) 43, 1883, 3354, 4570
MroNI GCCGGC 1 cut(s) 1937
MroXI GAANNNNTTC 3 cut(s) 2187, 3461, 3855
MscI TGGCCA 4 cut(s) 43, 1883, 3354, 4570
MslI CAYNNNNRTG 7 cut(s) 1403, 2112, 2201, 2520, 3794, 4053, 4473
Msp20I TGGCCA 4 cut(s) 43, 1883, 3354, 4570
MspA1I CMGCKG 2 cut(s) 533, 2345
MspI CCGG 3 cut(s) 961, 1938, 4623
MspR9I CCNGG 5 cut(s) 962, 1499, 2289, 2837, 3557
Mva1269I GAATGC 2 cut(s) 2150, 3656
MvaI CCWGG 4 cut(s) 1499, 2289, 2837, 3557
MvnI CGCG 1 cut(s) 1843
MwoI GCNNNNNNNGC 6 cut(s) 730, 908, 1177, 2048, 2213, 2348
NaeI GCCGGC 1 cut(s) 1939
NciI CCSGG 1 cut(s) 962
NgoMIV GCCGGC 1 cut(s) 1937
NlaIV GGNNCC 1 cut(s) 4554
NmuCI GTSAC 5 cut(s) 17, 82, 2282, 2953, 4220
NspI RCATGY 2 cut(s) 488, 4435
OliI CACNNNNGTG 1 cut(s) 2112
PaeI GCATGC 1 cut(s) 4435
PaeR7I CTCGAG 2 cut(s) 1490, 4416
PagI TCATGA 3 cut(s) 1861, 2857, 4054
PceI AGGCCT 1 cut(s) 4586
PciI ACATGT 1 cut(s) 484
PcsI WCGNNNNNNNCGW 2 cut(s) 1090, 2653
PctI GAATGC 2 cut(s) 2150, 3656
PdiI GCCGGC 1 cut(s) 1939
PdmI GAANNNNTTC 3 cut(s) 2187, 3461, 3855
PfoI TCCNGGA 1 cut(s) 1497
PleI GAGTC 5 cut(s) 866, 1080, 1167, 2218, 4155
PmaCI CACGTG 1 cut(s) 2129
PmlI CACGTG 1 cut(s) 2129
PpsI GAGTC 5 cut(s) 866, 1080, 1167, 2218, 4155
Ppu21I YACGTR 2 cut(s) 1685, 2129
PscI ACATGT 1 cut(s) 484
PshAI GACNNNNGTC 1 cut(s) 4361
PshBI ATTAAT 1 cut(s) 1119
PsiI TTATAA 1 cut(s) 641
Psp6I CCWGG 4 cut(s) 1497, 2287, 2835, 3555
PspCI CACGTG 1 cut(s) 2129
PspEI GGTNACC 2 cut(s) 2282, 2953
PspFI CCCAGC 1 cut(s) 2303
PspGI CCWGG 4 cut(s) 1497, 2287, 2835, 3555
PspN4I GGNNCC 1 cut(s) 4554
PspPI GGNCC 3 cut(s) 1228, 3559, 4192
PspXI VCTCGAGB 1 cut(s) 1490
PstI CTGCAG 1 cut(s) 2182
PstNI CAGNNNCTG 3 cut(s) 314, 2213, 3475
PsuI RGATCY 4 cut(s) 73, 589, 1147, 2540
PvuII CAGCTG 2 cut(s) 533, 2345
RseI CAYNNNNRTG 7 cut(s) 1403, 2112, 2201, 2520, 3794, 4053, 4473
SalI GTCGAC 1 cut(s) 1083
Sau96I GGNCC 3 cut(s) 1228, 3559, 4192
SchI GAGTC 5 cut(s) 867, 1080, 1168, 2219, 4156
ScrFI CCNGG 5 cut(s) 962, 1499, 2289, 2837, 3557
SduI GDGCHC 1 cut(s) 1491
SfcI CTRYAG 4 cut(s) 2178, 2706, 3176, 3987
Sfr274I CTCGAG 2 cut(s) 1490, 4416
SinI GGWCC 1 cut(s) 4192
SlaI CTCGAG 2 cut(s) 1490, 4416
SmiMI CAYNNNNRTG 7 cut(s) 1403, 2112, 2201, 2520, 3794, 4053, 4473
SmlI CTYRAG 5 cut(s) 496, 853, 1490, 2498, 4416
SmoI CTYRAG 5 cut(s) 496, 853, 1490, 2498, 4416
SpeI ACTAGT 1 cut(s) 86
SphI GCATGC 1 cut(s) 4435
SseBI AGGCCT 1 cut(s) 4586
SsiI CCGC 8 cut(s) 737, 841, 1381, 2141, 2982, 2985, 4460, 4601
SspI AATATT 3 cut(s) 2187, 2768, 4064
SspMI CTAG 7 cut(s) 87, 2156, 2321, 2610, 4152, 4532, 4630
StuI AGGCCT 1 cut(s) 4586
StyD4I CCNGG 5 cut(s) 960, 1497, 2287, 2835, 3555
StyI CCWWGG 4 cut(s) 51, 4069, 4134, 4151
TaiI ACGT 4 cut(s) 108, 1096, 1687, 2131
TatI WGTACW 8 cut(s) 1128, 1504, 1512, 1768, 2721, 2884, 3511, 3757
TauI GCSGC 4 cut(s) 844, 2987, 4463, 4604
TseFI GTSAC 5 cut(s) 17, 82, 2282, 2953, 4220
Tsp45I GTSAC 5 cut(s) 17, 82, 2282, 2953, 4220
TspGWI ACGGA 1 cut(s) 2829
VpaK11BI GGWCC 1 cut(s) 4192
VspI ATTAAT 1 cut(s) 1119
XagI CCTNNNNNAGG 2 cut(s) 2356, 3566
XceI RCATGY 2 cut(s) 488, 4435
XcmI CCANNNNNNNNNTGG 1 cut(s) 4174
XhoI CTCGAG 2 cut(s) 1490, 4416
XmaJI CCTAGG 1 cut(s) 4151
XmiI GTMKAC 3 cut(s) 1084, 2877, 4502
XmnI GAANNNNTTC 3 cut(s) 2187, 3461, 3855
XspI CTAG 7 cut(s) 87, 2156, 2321, 2610, 4152, 4532, 4630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.