MD09G1274900.v1.1

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000223
Physical Location & Seq
Reverse (-)
35207995 .. 35216428
8434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1274900.v1.1.491

Sequence Viewer

Length: 5331 bp
ATGTCTGCTTCCGACAGTTTCATGGATACTGTGTTTTCTTGGTCACTTGAAGACATTTTCAATGACAATCTGTACAAAAACCAGGTGGAGAAGATTCCTGAATCATTCAATTCAGTTCGGCATTATTTTGGGTCCTATTCATTTCCTTTATTAGAGGAAACTCGTGCACAAGTGCAGTCGAGTATGGAAACTATTGACAGAGCACCATTTGCTGTAGCAGTTGCTTTTAAAGAATCCAAGTCCAAGTTAGATGAAACATGGATATATAATATCAAGGTTGATGAGTGGCGAAACAGGTTCAGCGAACGTGGAAAGGAACCATACAAAACTTTGCCTGGGGATCTTTTTGTCTTAGCTGATGCGAAACCCGAAACTGTTTCTGATTTACAAAGGGTAGGGAGGTCATGGACTTTTGTATCGGTCACTAACATCACGGAAAATAAAATCTCAGAAAATAAGAGAGAAGATGAATTTAATGATGAGATAATAGAGACTGAAATAAAAGAGTTTGAAGTCAAGGCATCAAAAAGGTTTAAAGTCACAGATAACAAGAGCACTCCGGCATCACAATTTGTTGTTTTCCTTGTGAATTTGATCACAAATGGCAGAATATGGAAAGCATTGCACATGTTCGGAAATCTGAAGATAATCAAAGAGGTTCTGTGCACTGATTCTGTGGCTCAGAAAAACTATCCTCCCTCGTATGAAGTGAATGCTGACATTAGGGATAAATTGTTAGTTGAGAATTCATGTTCTGGATTGAATGAGTCACAAACTGGGGCAGTTTTGGCCTGTCTTGAAATGCTGTTTTGTGATTCCAAGTCTACATTGCAACTCATTTGGGGTCCACCTGGGACTGGGAAAACTAAATCAACTGCTACTCTGCTATTGACCCTGTTAAGGATGAATTGTAGGACTCTTGTTTGTGCCCCAACAAATGTTGCAGTAACTGAAGTAGCTTCTCATCTTGTAGAGATGGTGACTGAAGCAGAGTCCAATGCTTTGTTTTGTCCTCTGGGAAAAATTCTTTTATTTGGGAATAAAGAGGGACTCAAAGTTGGTTCTGATATTGAAGATATATTTTTGGATCACCGTATTGAAAGTCTTTTTGAGTGCCTGGGGCCGGTGACAGGTTGGAGTAGTTGCTCGTCTTCCATGATTGGACTTCTTGAGGATTGTGTTTCACATTACCATATTTTCTTGGAAAATGAGTTAGCCAAAGAGGAAGAACAAAATGGTGAGGGTGAAATGATAGAGAAAGGATGCGGAAGTGGGACCGAAGTTAGCAAGGGGAAATGCAAATCGTTTCTTGAATTTTTCAGAGAGAGATTTGTTTCTACTGCATTACCCTTAAAATGTTTTATTTCTACCTTCTGTACCCATATAAGCCAAGATTACATTTCGGCACAAAATTTCCAAGACATGATTTCGCTTTTAAGGTTAGTTGATTCCATTGAAATACAATTGTTGCAAGGTAATGTTGATTCGGAAGCATTGGAAGAACTTTTCTCTTGTTCAGAAGTTGAAGATGTGCCTGAGCCAGTAGACAACACATTTTATCTGTACATGAAGAGAAGAGAGTGCCTTTCAGTATTACTTACTCTTCAGGATTCAATTAGAAGACTTTACCTTCCAGATGTTACGGACAAAAAATCTGTAATGAAGTTTTGTTTTCAAAGAGCTTCCTTAATATTTTGCACTGCATCCAGTTCGTTTAAGCTGCATAGAGTGAAAATGGAGCCACTGACTATTGTTTTTATCGATGAGGCTGCACAATTGAAAGAGTGTGAGTCAGCGATACCCCTGCAACTTCCAGGTGTGAAGCATGCCGTTCTTGTTGGTGATGAATGTCAGTTACCAGCTATGGTGAAAAGCAATGTGTCCTATGAAGCTGGTTTTGGAAGAAGCTTGTTTGAGAGGTTGAGCTCGATGGGTCACTCTAAACATCTTCTAAACATGCAATACAGAATGCATCCGTCAATAAGTTCCTTCCCAAATTCAAGTATCTACTATAACCAGATCCTGGACGCTCCGAATGTTAAAAGAAGAAGCCATGAGAAACACTATCTTCCAGGGTCAATGTTCGGTCCTTATTCTTTTATTAATGTAATTGATGGAAGAGAGGAGAAGGACGAGGATGGACATAGTCTAAAGAATATGGTAGAGGTTGCAATTGTTTTGAAAATACTGCAGAATTTGCACAAAAAATGGGTTGTTTCAAAAGAGAAGCTTAGTATCGGTGTAATATCTCCTTATGCAGCTCAAGTAGCTGCACTTCAGGACAAACTTGGACAGAAGTACAATAAGCTAGATGGCTTTAAAGTGAAGGTGAAGACAGTTGATGGGTTCCAGGGTGGGGAAGAGGACATAATTATCATGTCCACTGTGCGATCCAATCAACATCACTCACTCGGTTTTATATCGAATCCACAAAGAGTAAATGTTGCTCTTACTAGGGCTCGGCACTGCCTTTGGATTTTGGGAAATGAAAGAACTCTTTGTGGTAGCGAATCTGTTTGGGAGGATTTGGTCCTTGATGCCAAGAATCGGCGGTGTTTCTTTAAAGCTGATGAAGACAAGGATATGGCCAAGGCTATATTAAAGGCAAAGAAAGAGTTAGACCAGTTTGATGATTTGCTTAATGCTAACAGCGGACTTTTCAGAAGTTCTAGATGGAAGGTGCTTTTCAGTGATAATTTTCTGAAGTCATTCAGAAAGCTTAGGTCGATTCAGTTGAAGAAGTCCGTGCTAAATCTTCTACTGAAGCTTTCCTGTGGATGGAGACCTAAAAAGGAATATATTCCGACTAGTTCTGGCTCATCAATCTTGAGGCAATATAAAGTTAGAGGTCTATATATTGTTTGCACAATTGACATTGCAAAGGAGTTGAAATACATTCAAGTGTTGAAGATTTGGAACATATTGCCTCTAGAGGGCATCCCAAAATTGATAAATCGTTTGGATAGTATTTTCAAGAGATATACAGATGATTTTATCAATCGCTGCAATGAGAAATGTCTTGAGGGTGATTTGGAAGTTCCGAAGTGCTGGCCGACGTCTTTGGATGTGCCTCGGTTTAAAGATCTTAGTGCCACTGAAACCGATAGTGATTTTGTTGGTGATGCTTCTGATGGTAGAAGTTATGTTGAGAATTCACAGGTCAGCGAGAGTTTGTTGCTCATGAAGTTTTATGCCTTGTCATCTGGTGTAGTGAATCACTTGCTGTCTGACCGTGAGGGTAAAGAGTTGGATCTTCCATTTGAAGTAACTGACCAGGAAATGGAGATTATTCTTTATCATAAGAGTACCTTTATAGTTGGACGGTCAGGAACGGGGAAGACAACAGTTTTAACCATGAAATTATTTCAAAATGAACAATGTTACCATTTGGCAGAGCAAGGATGCGTTAGTAGTCCAAATAGCATGGTTGAGCCGAGTTCTTCGGCTATTCGGGGGAGAAATCTACACCAACTTTTTGTTACCGTGAGTCCTAAGCTATGTTTTGCCATAAAACAACATGTTGTACACTTGAAAAGATTTGCATGTGGTGGAAGTGATTCGACTAAGAAATTTTTGATTGATATGGCTGATTTTGATGAGGAGGAATTTCAATTCAAGGATATCAAGGATTCATTTCAGGATATTCCCCCCGATTCTTACCCTCTTGTAATAACATTTCATAAGTTCTTGATGATGCTTGATGGAACACTGACTAACTCATACTTTGAAAGATTCCCTGAAGCAACAACACTCCCTCATGGTCATTTGAGAAGCTCAAGATCAGTTGCATTGCAGACCATCTGGACAAAGGAGGTTGATTATGAGAGGTTCAGTTCATCTTACTGGCCCCATTTTAATAAGCAGTTAACAAAGAAGCTTGATGCTTCAAGAGTCTTTACCGAGATTATATCCCATATTAAAGGTGGTTTAGGAGCCATAGAAGCAGGTGATGGCAGACTCAGTAGGGAGGATTATGTTCAACTGTCAGAGGGCCGGGTTTCCAATTTAAGCAGGCAAAAGAGAGAAGTAATATACGAAATATGTCAGGCTTATGAAAAAATGAAGTCGGAAAAAGGTGAATTTGATTTGGCTGATTTTGTCATTGATCTACACCGTCGGATCAGGCATGAAAAATTTGTAGGCGATCAAATGGATTTTGTTTATATTGATGAAGTTCAAGATCTCACCATGAGCCAAATTGCTCTGTTTAAGCATATGTGCAACAATGTCGAAGAGGGTTTTGTTTTTTCTGGTGATACGGCACAGACCATTGCCAGGGGAATTGATTTTAGGTTTGAAGATATGCGACATTTGTTCCACGAGAAGTTTTTATTGGAATCAAGAGGTAATAAGCAGGAAAGAAAAGAAAAGGGACAAATCTCAGAAATATTTCACTTGACTCAAAACTTCCGTACGCATGCTGGCGTTCTGAAATTATCACAGAGCATTGTTGAACTACTTTATCATTTTTTCCCTCAATCTATTGATGTTTTGAAACCTGAAACCAGTCTAATATATGGGGAAGCTCCAGTACTGCTTGAATCCAGAGATGAAAATGCAATCATAAAAATTTTTGGAAATAGTGGAACTGATAATTCTAACATAGTTGGCTTCGGAGCAGAGCAAGTTATTTTGGTGCGTGATGTTGGTGCTCAAAAAGAAATTTTCAAAATCGTTGGGAAGCAGGCTCTTGTCCTGACCATAGTGGAATGCAAGGGGCTTGAATTCCAGGATGTACTCTTGTACAACTTTTTTGGATCATCGCCACTAAAACAGCAATGGAGGGTGATATATGATTACATGAAGAAGAAAGATCTACTTGATTCAACATTGCCTCGACCTCCAAGTTTCCGTGAAGGGCACAACATCCTATGCTCTGAACTGAAGCAATTATATGTTGCTGTTACTCGGACAAGACAAAGATTATGGTTTTGCGAGAATGGAGAAGAGTTCTCCAAGCCCATGTTTGACTATTGGAAGAAGAAATGTCTTGTACAGGTTAGACAACTGGACGATTCCCTTGCGCAGGCAATGCAAGTTGCTAGTAGTCCTGAGGAGTGGAAATCACGAGGCATTAAGCTCTATCATGAACATAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGATACTTATTGGGAAAGGAGGTCCAAAGCTGCTGGCCTTAAAGCTAACGCCGATCGAATGCGTACTTCAAATCCTGCGGAGGTCAAGACCATTCTTAAGGAAGCTGCTGATATATTTGATGCTATAGGCAAGGCAGATTCCGCAGCTAGATGTTTTTCCGATTTGGGAGAGTATGAAAGAGCAGGTATGACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1777

Amino Acids

202.47

Weight (kDa)

6.12

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 69 - 214 5.4e-50 Domain of unknown function (DUF6469)
AAA_11 PF13086 254 - 626 1.3e-31 AAA domain
AAA_19 PF13245 273 - 622 1.1e-06 AAA domain
AAA_12 PF13087 634 - 830 2.6e-60 AAA domain
UvrD-helicase PF00580 1335 - 1424 1.5e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3935
AatII GACGTC 1 cut(s) 3059
Acc16I TGCGCA 1 cut(s) 5025
Acc36I ACCTGC 2 cut(s) 3935, 5306
AccB7I CCANNNNNTGG 2 cut(s) 2023, 3280
AccI GTMKAC 2 cut(s) 824, 1545
AciI CCGC 5 cut(s) 1266, 2551, 2652, 5210, 5274
AclWI GGATC 7 cut(s) 348, 1095, 2014, 2385, 3258, 4127, 4765
AcoI YGGCCR 3 cut(s) 2586, 3050, 5106
AcuI CTGAAG 9 cut(s) 662, 972, 1005, 1589, 2261, 2723, 2783, 3759, 4904
AcyI GRCGYC 1 cut(s) 3056
AdeI CACNNNGTG 1 cut(s) 5115
AflII CTTAAG 1 cut(s) 5228
AflIII ACRYGT 2 cut(s) 627, 3517
AhlI ACTAGT 1 cut(s) 2807
AjuI GAANNNNNNNTTGG 4 cut(s) 1881, 1913, 4316, 4348
Alw21I GWGCWC 6 cut(s) 169, 205, 557, 668, 1928, 4654
Alw26I GTCTC 2 cut(s) 485, 2776
Alw44I GTGCAC 2 cut(s) 165, 664
AlwI GGATC 7 cut(s) 348, 1095, 2014, 2385, 3258, 4127, 4765
AlwNI CAGNNNCTG 2 cut(s) 950, 2023
AoxI GGCC 8 cut(s) 789, 1121, 2586, 3050, 3845, 3991, 5106, 5167
ApaLI GTGCAC 2 cut(s) 165, 664
ApeKI GCWGC 8 cut(s) 1720, 1769, 2258, 2270, 3003, 5162, 5237, 5276
AseI ATTAAT 1 cut(s) 2103
Asp700I GAANNNNTTC 4 cut(s) 2708, 2799, 3556, 4389
AspLEI GCGC 1 cut(s) 5026
AspS9I GGNCC 9 cut(s) 132, 845, 1121, 1275, 2087, 2530, 3846, 3991, 5154
AsuC2I CCSGG 1 cut(s) 3995
AvaII GGWCC 6 cut(s) 132, 845, 1275, 2087, 2530, 5154
AxyI CCTNAGG 1 cut(s) 5052
BaeGI GKGCMC 4 cut(s) 169, 668, 931, 4863
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BalI TGGCCA 2 cut(s) 2588, 5108
BanII GRGCYC 2 cut(s) 1928, 2461
BarI GAAGNNNNNNTAC 4 cut(s) 2259, 2291, 4515, 4547
BauI CACGAG 3 cut(s) 162, 4319, 5067
BbsI GAAGAC 6 cut(s) 57, 1143, 1627, 2339, 2580, 3344
Bbv12I GWGCWC 6 cut(s) 169, 205, 557, 668, 1928, 4654
BbvI GCAGC 8 cut(s) 1707, 1756, 2257, 2270, 2990, 5149, 5224, 5288
BceAI ACGGC 2 cut(s) 1814, 4275
BciVI GTATCC 1 cut(s) 19
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 1 cut(s) 3995
BcoDI GTCTC 2 cut(s) 485, 2776
BcuI ACTAGT 1 cut(s) 2807
BfaI CTAG 7 cut(s) 2309, 2454, 2670, 2808, 2930, 5043, 5280
BfmI CTRYAG 3 cut(s) 213, 2189, 5256
BfrI CTTAAG 1 cut(s) 5228
BfuAI ACCTGC 2 cut(s) 3935, 5306
BfuI GTATCC 1 cut(s) 19
BglII AGATCT 3 cut(s) 3082, 4180, 4813
BisI GCNGC 8 cut(s) 1721, 1770, 2259, 2271, 3004, 5163, 5238, 5277
BlsI GCNGC 8 cut(s) 1722, 1771, 2260, 2272, 3005, 5164, 5239, 5278
BmcAI AGTACT 1 cut(s) 4533
Bme18I GGWCC 6 cut(s) 132, 845, 1275, 2087, 2530, 5154
BmgT120I GGNCC 9 cut(s) 132, 845, 1121, 1275, 2087, 2530, 3846, 3991, 5154
BmiI GGNNCC 9 cut(s) 133, 318, 846, 1122, 1276, 1740, 2348, 3848, 3934
BmrI ACTGGG 2 cut(s) 786, 867
BmuI ACTGGG 2 cut(s) 786, 867
BpiI GAAGAC 6 cut(s) 57, 1143, 1627, 2339, 2580, 3344
BpmI CTGGAG 1 cut(s) 4512
Bpu10I CCTNAGC 3 cut(s) 1536, 2720, 3492
BpuEI CTTGAG 5 cut(s) 1190, 2247, 2848, 3041, 3760
BpuMI CCSGG 1 cut(s) 3995
Bsa29I ATCGAT 1 cut(s) 1761
BsaHI GRCGYC 1 cut(s) 3056
BsaI GGTCTC 1 cut(s) 2776
BsaJI CCNNGG 8 cut(s) 335, 851, 1117, 2072, 2350, 2589, 3071, 4275
Bse118I RCCGGY 1 cut(s) 1123
Bse1I ACTGG 9 cut(s) 781, 862, 1541, 1707, 2623, 3848, 4506, 4529, 5013
Bse21I CCTNAGG 1 cut(s) 5052
BseCI ATCGAT 1 cut(s) 1761
BseDI CCNNGG 8 cut(s) 335, 851, 1117, 2072, 2350, 2589, 3071, 4275
BseMII CTCAG 6 cut(s) 462, 695, 1527, 3973, 4395, 5043
BseNI ACTGG 9 cut(s) 781, 862, 1541, 1707, 2623, 3848, 4506, 4529, 5013
BseRI GAGGAG 3 cut(s) 2138, 3614, 5069
BseSI GKGCMC 4 cut(s) 169, 668, 931, 4863
BseXI GCAGC 8 cut(s) 1707, 1756, 2257, 2270, 2990, 5149, 5224, 5288
BsgI GTGCAG 3 cut(s) 194, 1755, 2256
Bsh1285I CGRYCG 1 cut(s) 5188
BshFI GGCC 8 cut(s) 791, 1123, 2588, 3052, 3847, 3993, 5108, 5169
BshVI ATCGAT 1 cut(s) 1761
BsiEI CGRYCG 1 cut(s) 5188
BsiHKAI GWGCWC 6 cut(s) 169, 205, 557, 668, 1928, 4654
BsiSI CCGG 3 cut(s) 560, 1124, 3994
BsiWI CGTACG 1 cut(s) 4412
BslFI GGGAC 4 cut(s) 868, 1062, 1288, 4386
BsmAI GTCTC 2 cut(s) 485, 2776
BsmFI GGGAC 4 cut(s) 868, 1062, 1288, 4386
BsmI GAATGC 4 cut(s) 718, 1974, 4715, 5196
BsnI GGCC 8 cut(s) 791, 1123, 2588, 3052, 3847, 3993, 5108, 5169
Bso31I GGTCTC 1 cut(s) 2776
Bsp1286I GDGCHC 9 cut(s) 169, 205, 557, 668, 931, 1928, 2461, 4654, 4863
Bsp1407I TGTACA 5 cut(s) 72, 1563, 3523, 4743, 4993
BspACI CCGC 5 cut(s) 1266, 2551, 2652, 5210, 5274
BspANI GGCC 8 cut(s) 791, 1123, 2588, 3052, 3847, 3993, 5108, 5169
BspCNI CTCAG 6 cut(s) 461, 694, 1528, 3972, 4394, 5044
BspDI ATCGAT 1 cut(s) 1761
BspHI TCATGA 2 cut(s) 3180, 5086
BspLI GGNNCC 9 cut(s) 133, 318, 846, 1122, 1276, 1740, 2348, 3848, 3934
BspMAI CTGCAG 1 cut(s) 2193
BspMI ACCTGC 2 cut(s) 3935, 5306
BspPI GGATC 7 cut(s) 348, 1095, 2014, 2385, 3258, 4127, 4765
BspTI CTTAAG 1 cut(s) 5228
BspTNI GGTCTC 1 cut(s) 2776
BsrFI RCCGGY 1 cut(s) 1123
BsrGI TGTACA 5 cut(s) 72, 1563, 3523, 4743, 4993
BsrI ACTGG 9 cut(s) 781, 862, 1541, 1707, 2623, 3848, 4506, 4529, 5013
BssAI RCCGGY 1 cut(s) 1123
BssECI CCNNGG 8 cut(s) 335, 851, 1117, 2072, 2350, 2589, 3071, 4275
BssNI GRCGYC 1 cut(s) 3056
BssSI CACGAG 3 cut(s) 162, 4319, 5067
BssT1I CCWWGG 1 cut(s) 2589
Bst2BI CACGAG 3 cut(s) 162, 4319, 5067
Bst6I CTCTTC 7 cut(s) 1565, 1570, 1608, 2113, 2355, 4227, 4941
BstACI GRCGYC 1 cut(s) 3056
BstAFI CTTAAG 1 cut(s) 5228
BstAPI GCANNNNNTGC 3 cut(s) 209, 2197, 5032
BstAUI TGTACA 5 cut(s) 72, 1563, 3523, 4743, 4993
BstC8I GCNNGC 8 cut(s) 1827, 3050, 4013, 4419, 4423, 4686, 5028, 5167
BstENI CCTNNNNNAGG 1 cut(s) 5024
BstHHI GCGC 1 cut(s) 5026
BstMAI GTCTC 2 cut(s) 485, 2776
BstMCI CGRYCG 1 cut(s) 5188
BstMWI GCNNNNNNNGC 7 cut(s) 209, 788, 2197, 2267, 3941, 5032, 5273
BstNSI RCATGY 6 cut(s) 631, 1829, 1960, 3521, 3546, 4421
BstSFI CTRYAG 3 cut(s) 213, 2189, 5256
BstSLI GKGCMC 4 cut(s) 169, 668, 931, 4863
BstV1I GCAGC 8 cut(s) 1707, 1756, 2257, 2270, 2990, 5149, 5224, 5288
BstV2I GAAGAC 6 cut(s) 57, 1143, 1627, 2339, 2580, 3344
BstX2I RGATCY 6 cut(s) 340, 2019, 3082, 3250, 4180, 4813
BstYI RGATCY 6 cut(s) 340, 2019, 3082, 3250, 4180, 4813
Bsu15I ATCGAT 1 cut(s) 1761
Bsu36I CCTNAGG 1 cut(s) 5052
BsuI GTATCC 1 cut(s) 19
BsuRI GGCC 8 cut(s) 791, 1123, 2588, 3052, 3847, 3993, 5108, 5169
BsuTUI ATCGAT 1 cut(s) 1761
BtgZI GCGATG 1 cut(s) 4746
BtsI GCAGTG 2 cut(s) 1698, 2464
BtsIMutI CAGTG 8 cut(s) 666, 1698, 1742, 2382, 2464, 2695, 3093, 3707
BveI ACCTGC 2 cut(s) 3935, 5306
Cac8I GCNNGC 8 cut(s) 1827, 3050, 4013, 4419, 4423, 4686, 5028, 5167
CaiI CAGNNNCTG 2 cut(s) 950, 2023
CciI TCATGA 2 cut(s) 3180, 5086
CfoI GCGC 1 cut(s) 5026
Cfr10I RCCGGY 1 cut(s) 1123
Cfr13I GGNCC 9 cut(s) 132, 845, 1121, 1275, 2087, 2530, 3846, 3991, 5154
ClaI ATCGAT 1 cut(s) 1761
CseI GACGC 1 cut(s) 2036
CsiI ACCWGGT 1 cut(s) 81
DraI TTTAAA 5 cut(s) 229, 535, 2320, 2563, 3079
DraIII CACNNNGTG 1 cut(s) 5115
EaeI YGGCCR 3 cut(s) 2586, 3050, 5106
Eam1104I CTCTTC 7 cut(s) 1565, 1570, 1608, 2113, 2355, 4227, 4941
EarI CTCTTC 7 cut(s) 1565, 1570, 1608, 2113, 2355, 4227, 4941
Ecl136II GAGCTC 1 cut(s) 1926
Eco130I CCWWGG 1 cut(s) 2589
Eco24I GRGCYC 2 cut(s) 1928, 2461
Eco31I GGTCTC 1 cut(s) 2776
Eco32I GATATC 1 cut(s) 3622
Eco47I GGWCC 6 cut(s) 132, 845, 1275, 2087, 2530, 5154
Eco53kI GAGCTC 1 cut(s) 1926
Eco57I CTGAAG 9 cut(s) 662, 972, 1005, 1589, 2261, 2723, 2783, 3759, 4904
Eco81I CCTNAGG 1 cut(s) 5052
EcoICRI GAGCTC 1 cut(s) 1926
EcoNI CCTNNNNNAGG 1 cut(s) 5024
EcoO109I RGGNCCY 1 cut(s) 132
EcoRI GAATTC 3 cut(s) 745, 3151, 4724
EcoRV GATATC 1 cut(s) 3622
EcoT14I CCWWGG 1 cut(s) 2589
EcoT22I ATGCAT 1 cut(s) 1974
EcoT38I GRGCYC 2 cut(s) 1928, 2461
ErhI CCWWGG 1 cut(s) 2589
FalI AAGNNNNNCTT 6 cut(s) 2214, 2246, 3293, 3325, 4803, 4835
FaqI GGGAC 4 cut(s) 868, 1062, 1288, 4386
FauNDI CATATG 1 cut(s) 4215
FbaI TGATCA 1 cut(s) 594
FblI GTMKAC 2 cut(s) 824, 1545
Fnu4HI GCNGC 8 cut(s) 1721, 1770, 2259, 2271, 3004, 5163, 5238, 5277
FriOI GRGCYC 2 cut(s) 1928, 2461
Fsp4HI GCNGC 8 cut(s) 1721, 1770, 2259, 2271, 3004, 5163, 5238, 5277
FspBI CTAG 7 cut(s) 2309, 2454, 2670, 2808, 2930, 5043, 5280
FspI TGCGCA 1 cut(s) 5025
GlaI GCGC 1 cut(s) 5025
GluI GCNGC 8 cut(s) 1721, 1770, 2259, 2271, 3004, 5163, 5238, 5277
GsuI CTGGAG 1 cut(s) 4512
HaeIII GGCC 8 cut(s) 791, 1123, 2588, 3052, 3847, 3993, 5108, 5169
HapII CCGG 3 cut(s) 560, 1124, 3994
HgaI GACGC 1 cut(s) 2036
HhaI GCGC 1 cut(s) 5026
Hin1I GRCGYC 1 cut(s) 3056
Hin6I GCGC 1 cut(s) 5024
HinP1I GCGC 1 cut(s) 5024
HincII GTYRAC 1 cut(s) 3867
HindII GTYRAC 1 cut(s) 3867
HindIII AAGCTT 5 cut(s) 1906, 2228, 2717, 2765, 3875
HpaI GTTAAC 1 cut(s) 3867
HpaII CCGG 3 cut(s) 560, 1124, 3994
Hpy166II GTNNAC 8 cut(s) 167, 666, 825, 848, 1546, 2382, 3526, 3867
Hpy8I GTNNAC 8 cut(s) 167, 666, 825, 848, 1546, 2382, 3526, 3867
Hpy99I CGWCG 2 cut(s) 3058, 4119
HpyAV CCTTC 7 cut(s) 1381, 1640, 1999, 2122, 2320, 2671, 4850
HpyCH4IV ACGT 2 cut(s) 307, 3056
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 788, 2197, 2267, 3941, 5032, 5273
HpySE526I ACGT 2 cut(s) 307, 3056
Hsp92I GRCGYC 1 cut(s) 3056
HspAI GCGC 1 cut(s) 5024
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 3867
LmnI GCTCC 5 cut(s) 1738, 2035, 3932, 4531, 4616
Lsp1109I GCAGC 8 cut(s) 1707, 1756, 2257, 2270, 2990, 5149, 5224, 5288
MabI ACCWGGT 1 cut(s) 81
MaeI CTAG 7 cut(s) 2309, 2454, 2670, 2808, 2930, 5043, 5280
MaeII ACGT 2 cut(s) 307, 3056
MfeI CAATTG 4 cut(s) 1463, 1775, 2172, 2868
MflI RGATCY 6 cut(s) 340, 2019, 3082, 3250, 4180, 4813
MhlI GDGCHC 9 cut(s) 169, 205, 557, 668, 931, 1928, 2461, 4654, 4863
MlsI TGGCCA 2 cut(s) 2588, 5108
MluNI TGGCCA 2 cut(s) 2588, 5108
MmeI TCCRAC 7 cut(s) 36, 1115, 2828, 3228, 3298, 4047, 4097
Mox20I TGGCCA 2 cut(s) 2588, 5108
Mph1103I ATGCAT 1 cut(s) 1974
MroXI GAANNNNTTC 4 cut(s) 2708, 2799, 3556, 4389
MscI TGGCCA 2 cut(s) 2588, 5108
MslI CAYNNNNRTG 1 cut(s) 2900
Msp20I TGGCCA 2 cut(s) 2588, 5108
MspA1I CMGCKG 1 cut(s) 2652
MspCI CTTAAG 1 cut(s) 5228
MspI CCGG 3 cut(s) 560, 1124, 3994
MunI CAATTG 4 cut(s) 1463, 1775, 2172, 2868
Mva1269I GAATGC 4 cut(s) 718, 1974, 4715, 5196
MwoI GCNNNNNNNGC 7 cut(s) 209, 788, 2197, 2267, 3941, 5032, 5273
NciI CCSGG 1 cut(s) 3995
NdeI CATATG 1 cut(s) 4215
NlaIV GGNNCC 9 cut(s) 133, 318, 846, 1122, 1276, 1740, 2348, 3848, 3934
NmeAIII GCCGAG 2 cut(s) 2440, 3459
NmuCI GTSAC 7 cut(s) 42, 421, 538, 768, 979, 1126, 1934
NsbI TGCGCA 1 cut(s) 5025
NsiI ATGCAT 1 cut(s) 1974
NspI RCATGY 6 cut(s) 631, 1829, 1960, 3521, 3546, 4421
PaeI GCATGC 2 cut(s) 1829, 4421
PagI TCATGA 2 cut(s) 3180, 5086
PaqCI CACCTGC 1 cut(s) 3935
PciI ACATGT 2 cut(s) 627, 3517
PctI GAATGC 4 cut(s) 718, 1974, 4715, 5196
PdmI GAANNNNTTC 4 cut(s) 2708, 2799, 3556, 4389
Pfl23II CGTACG 1 cut(s) 4412
PflFI GACNNNGTC 1 cut(s) 2145
PflMI CCANNNNNTGG 2 cut(s) 2023, 3280
PfoI TCCNGGA 2 cut(s) 2022, 4728
PkrI GCNGC 8 cut(s) 1722, 1771, 2260, 2272, 3005, 5164, 5239, 5278
Ple19I CGATCG 1 cut(s) 5188
PpuMI RGGWCCY 1 cut(s) 132
PscI ACATGT 2 cut(s) 627, 3517
PshBI ATTAAT 1 cut(s) 2103
Psp124BI GAGCTC 1 cut(s) 1928
Psp5II RGGWCCY 1 cut(s) 132
PspLI CGTACG 1 cut(s) 4412
PspN4I GGNNCC 9 cut(s) 133, 318, 846, 1122, 1276, 1740, 2348, 3848, 3934
PspPI GGNCC 9 cut(s) 132, 845, 1121, 1275, 2087, 2530, 3846, 3991, 5154
PspPPI RGGWCCY 1 cut(s) 132
PstI CTGCAG 1 cut(s) 2193
PstNI CAGNNNCTG 2 cut(s) 950, 2023
PsuI RGATCY 6 cut(s) 340, 2019, 3082, 3250, 4180, 4813
PsyI GACNNNGTC 1 cut(s) 2145
PvuI CGATCG 1 cut(s) 5188
RseI CAYNNNNRTG 1 cut(s) 2900
SacI GAGCTC 1 cut(s) 1928
SatI GCNGC 8 cut(s) 1721, 1770, 2259, 2271, 3004, 5163, 5238, 5277
Sau96I GGNCC 9 cut(s) 132, 845, 1121, 1275, 2087, 2530, 3846, 3991, 5154
ScaI AGTACT 1 cut(s) 4533
SduI GDGCHC 9 cut(s) 169, 205, 557, 668, 931, 1928, 2461, 4654, 4863
SexAI ACCWGGT 1 cut(s) 81
SfcI CTRYAG 3 cut(s) 213, 2189, 5256
SinI GGWCC 6 cut(s) 132, 845, 1275, 2087, 2530, 5154
SmiMI CAYNNNNRTG 1 cut(s) 2900
SmlI CTYRAG 6 cut(s) 1169, 2262, 2827, 3020, 3775, 5228
SmoI CTYRAG 6 cut(s) 1169, 2262, 2827, 3020, 3775, 5228
SpeI ACTAGT 1 cut(s) 2807
SphI GCATGC 2 cut(s) 1829, 4421
SsiI CCGC 5 cut(s) 1266, 2551, 2652, 5210, 5274
SspI AATATT 2 cut(s) 1692, 4389
SspMI CTAG 7 cut(s) 2309, 2454, 2670, 2808, 2930, 5043, 5280
SstI GAGCTC 1 cut(s) 1928
StyI CCWWGG 1 cut(s) 2589
TaiI ACGT 2 cut(s) 310, 3059
TaqI TCGA 9 cut(s) 179, 1761, 1928, 2423, 2726, 3560, 4230, 4837, 5188
TaqII GACCGA 3 cut(s) 409, 1292, 2075
TatI WGTACW 8 cut(s) 72, 1563, 2298, 3523, 4531, 4735, 4743, 4993
TscAI CASTG 8 cut(s) 673, 1705, 1749, 2389, 2471, 2695, 3100, 3714
TseFI GTSAC 7 cut(s) 42, 421, 538, 768, 979, 1126, 1934
TseI GCWGC 8 cut(s) 1720, 1769, 2258, 2270, 3003, 5162, 5237, 5276
Tsp45I GTSAC 7 cut(s) 42, 421, 538, 768, 979, 1126, 1934
TspGWI ACGGA 6 cut(s) 449, 1658, 1965, 2734, 4400, 4841
TspRI CASTG 8 cut(s) 673, 1705, 1749, 2389, 2471, 2695, 3100, 3714
Tth111I GACNNNGTC 1 cut(s) 2145
Van91I CCANNNNNTGG 2 cut(s) 2023, 3280
Vha464I CTTAAG 1 cut(s) 5228
VneI GTGCAC 2 cut(s) 165, 664
VpaK11BI GGWCC 6 cut(s) 132, 845, 1275, 2087, 2530, 5154
VspI ATTAAT 1 cut(s) 2103
XagI CCTNNNNNAGG 1 cut(s) 5024
XbaI TCTAGA 2 cut(s) 2669, 2929
XceI RCATGY 6 cut(s) 631, 1829, 1960, 3521, 3546, 4421
XcmI CCANNNNNNNNNTGG 1 cut(s) 3920
XmiI GTMKAC 2 cut(s) 824, 1545
XmnI GAANNNNTTC 4 cut(s) 2708, 2799, 3556, 4389
XspI CTAG 7 cut(s) 2309, 2454, 2670, 2808, 2930, 5043, 5280
ZraI GACGTC 1 cut(s) 3057
ZrmI AGTACT 1 cut(s) 4533
Zsp2I ATGCAT 1 cut(s) 1974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.