Rroxscaffold_5G00386410

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000223
Physical Location & Seq
Forward (+)
65292630 .. 65303954
11325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386410.1

Sequence Viewer

Length: 7971 bp
ATGGAGGTTTCCGGCAAGAAAAAGATCAAGCCTGACCCTTTCACTGATACTGTGTTTTCTTGGTCACTGGAGGACATTTTCAATGAGGATCTTTACAAAGACAAGGTGAAAAAGATTCCTGTATCATTTCAATCGGCTGAGCATTACTTTGGGTCTTATATATATCCTTTGCTGGAAGAAACCCGAGCAGAAGTGCATTCAAGTATGGAAACTCTTAATAGAGCACCATTTGCTGAAGTAGTGGGTTTTGAAGAAGCCAAGCCATATGGAACAAAGCTATATGATGTCAAGGTTGATTACTGGCGAAACAGGTCATGGGCTTTTGCATCAGCCACTAATGTCTCAGAAAGTGAGAATGAAGATGACAGTACCTCTCTTTATTTTAAAGTCAAGGCATCAGAAGAGTTTGAAGTTGAAAATAGCAGGACATCACTTTTTATAGTTTTCTTGGTGAATTTAATCCCTGAAGGAAGAATTTGGAAAGCGTTGCACATGTCCAGAAATCTTAAGATTATCAATGAAGTTCTATGCCCTGAATCTATGGTTCAGAAAGATTTCTGCTCTGAAAGGAATAATGACTGGAATCAGAGTATAGTTGAGAGTTTATCATCTGGATTGAATGAATCCCAAACTGGGGCAGTGTTGGCCTGCCTTGAAATGCTGCTTCCTGGTGAAAAGTCTGCCGTGGAACTTATCTGGGGTCCTCCTGGTACTGGAAAAACAAAAGCCACAGTTACTCTGCTTTCAACCCTGCTACAGATGAATTGTAGGACTCTTATTTGTGCTCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGTCTTGTGAAGATGGTGAGTAAAGCAGAGCCAAATGATTTGTTTTGTTCTTTAGGAGAGATTCTTCTATTTGGGAATAAGGAGCGACTGAAAATTGGTTCAGAGATTGAAGAAATTTATATGGACTACCGTGTCAAAAGGCTTGTAGATTGTTTGGGGCCAAGGACTGGTTGGAGGCATTGTTTTGCTTCCATGATAGGTTTACTTGAGGATTGTGTTTCTGATTACCATATTTTCTTGGAAAATGAGTTCACGAAAGAGACAGAACAAATGAATGTTAGTGAAACGAACGATAAAGAATGCAGAAGAGGTGCTCAAGTTAAAAAGGGGAAGTGCAAAACATTTGTTCAATTTTTGAGAGGTAGATTTGCGTCTACTGCAACGCCGTTAAGATATGGCATTTCTGTGTTCTGTACCCATGTAGGGAAAAATTATATTTCGGTAGACAATTTCCAAGACTTGGTTTCGCTTATCCGGTTAGTTGATTCCTTTGAGTTACTGTTGTCTCAAGATAATGTTGTTTCTGAAGTGTTGGAAGAACTCTTTTCTCATTCGGATGTTGAAGATGTGTCTGAGTCGTTTGTAGGCAACTCTTTTAATCTTTGTATGATGAGAAGGGATTGCCTTTCAGCTTTACGCACTCTACGGGATTCTCTTAGTAAACTAAGACTTCCAAATATTACAAATGAGGAATCTATCCGGGAGTTCTGTTTTCAAAGAGCATCTCTTATATTTTGCACTGCATCTAGTTCGTATAAGCTGCATAGAGTGGCAATGGAACCACTAAGCATTGTTGTTATTGACGAGGCTGCACAGCTAAAAGAGTGTGAGTCCACAATACCTCTGCAACTTCGAGGTGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTGAGCGTAATGGGTCATTCAAAACATCTTTTAAATATGCAATACAGAATGCATCCATCAATAAGTTTATTCCCAAATTCAAATTTCTATTGTAACTTGATCCTGGATGCTCCAAATGTAAAACGAAGAATCTATGAGAAGCACTATCTTCCAGGAGCAATGTTTGGTCCTTATTCTTTTATAAATGTGATTGGTGGAAGAGAGGAGAAAGATGAGGATGGACATAGTCGGAAAAATATGGTGGAGGTTGCTATTGTTTCAAAAATACTGCGGAATCTGTACAAAGAATGGGTCAAATCAAAACAGAGACTCAGTATTGGGATAGTATCTCCCTATGCTGCTCAAGTAGTCGCAATTAGGGACAGAGTGGGTGAGAAGTATGATAGGCTTGATGGCTTTGTAGTGAAGGTAAAAACAGTTGATGGGTTTCAAGGTGGGGAAGAGGATATAATTGTAATGTCCACTGTACGATCCAACAGCCAGCAATCACTTGAATTCATTTCAAAATTTCAAAGAGTTAATGTCTCTATTACAAGGGCTAGGCACTGTTTGTGGATTTTGGGCAATGAAAAAACTCTTTCCGATAGTGAATCTGTTTGGAAGGCCTTGGTCCTTGATGCCAAAGGTCGTCAATGCTTCTTTAATGCCGATGAAGACAAGGATTTAGCCAAGGCCATATTAGAGGTAAAGAAAGAGTTTGACCAATTTGATGATTTGCTCAATGCCGACAGCATACTTTTTAGAAGTGCTAGATGGAAGGTACTTTTCAGTGATAACTTTCTGAAGTCATTCAAGAAACTGACATCAGTCCGCTTGAAGAAGTCAGTGCTCAATCTTCTACTGAAGCTTTCCAGTGGTTGGAGACCTAAGAAGCGAAATGTTGAGACAATATCTGGTAGCTCTTGCATGATCTTGAAGAAATTTAAGGTTGAAGGTCTATACATTGTTTGCACTAATGACATTGCAAAGAACCTGGAGTACGTTCAAGTATTAAAGATCTGGGACATATTACCTCTAGAGGATATCCCAAAGTTGATAAACCGCTTGGATAGTATTTTCAACAGATATACTGATAACTTTATTAATCTTTGCAGGGAGAAGTGTCTCGATGGTGATCTAGAGGTTCCAAAAAGCTGGTCACGGTCTTTGGAATTTTCCCGGTTTAAGGATCTTAGCATCAGTGAAGCTCTGAGTGATTTAGTTGGTGATACTTCTGATTGTAGAAGCTATGTTGAGAATTCACAGGTTAGTGAGAGTTTGTTGCTGATGAAATTTTATTCCTTATCATCTGGGGTAGTAAACCACTTGCTGTCGGACCGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACAGACGAAGAGATGGAGATTATCCTTTATCGTAGAAGTTCCTTTATAGTGGGACGGTCAGGAACTGGGAAAACCACAGTTTTAACCATGAAATTGTTTCAAAAAGAACAATGGCACCAATTTGCGGTTCAAGGATGCTATAGTAGTCAAAATACTAGCAAGGTTGAGCAGGGTTCTGCAGCTACTGAAGGGAAAGTTCTGCGCCAGCTTTTTGTGACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCGACATGTTTCACACTTGAAAAGTTTTGCATGCGGCGGAGGTAACTCAACTGACTCAACTGAAAGAGGTTTAATTGATATGGCTGATTTTGATGAGGAGGAAACCCAATTCAAGGATATCCAAGATTCATTTCATGATATTCCTCCGAGTTCTTACCCACTTGTCATTACATTCCATAAGTTTTTGATGATGCTTGATGGGACATTGAGTAACTCATACTTTGAAAGATTCCTTGATGTGACAGCTACTGTTGGCCATTTTAGGAGTTCGAGATCAGTTGCATTTCAGAGCTTGATAAGGACAAAAGAAGTTAATTATGAGAGGTTTAGCTCTTTGTATTGGCCCCGTTTTAATACTCAGATAACAAAGAAGCTTGACGTTTCAAGAGTCTTTATAGAGATTATTTCTTATATAAAAGGTGGCTTAGGAGCTATAGATGCATGTGATGGTAAACTCAGCAGGGAGGATTATGTTCAACTGTGCGAGGGCCGGGGTTCTAATTTGAGCAAGCAAAAGAGAGAAGCAATATACGATGTTTTTCAGGCGTATGAAAAGTTGAAGATGGAAAATGGTGAATTCGATCTGGCTGATTTTGTAATTGATCTTCACCGTCGACTCAAGCATGAAAAATATGAGGGTGATCAGATGGAATTTGTTTATATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTTAAACACATGTGCAACAATGTTGAAGAGGGATTTGTCTTTTCTGGTGATACAGCACAAACAATAGCAAGGGGTATTGACTTCAGGTTTCAGGATATACGACATCTGTTCTACAAGAAGTTTGTATTGGAGTCAAGAAGCAATAACAACGATAAAAGAAAAGAAAAAGGCGTCGTCTCTGAATTACTTCAGTTGACTCAAAACTTCCGTACTCATGCTGGTATATTGAAGTTATCACAAAGCATTGTTGAACTACTGTATCGGTTTTTCCCCTTTTCTGTTGATGTTTTAAAACCTGAAACCAGTCTGATTTATGGGGAAGCTCCAGTTTTGCTTGAATCTGGGGCAAACGAAAATGCAATCATGAAAATTTTTGGAAACAGTGGAAATATAGTTGGCTTTGGTGCAGAGCAGGTGATTTTGGTGCGAGATGATAGTGCTCGACGAGAAATATCCAAATTTGTAGGGAAGCACGCTCTTGTCCTAACCATAGTGGAGTGTAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTCGGCACATCACCTTTGAAAAATCAGTGGAGGGTCATTTATGATTACATGAAGGAACAAGATTTACTTGACTCCACATTACCTAAGCGCTTTCCAAGTTTCAGTGAATCAAAGCACAACATATTGTGCTCAGAACTGAAGCAATTATATGTGGCTGTTACTCGAACAAGACAGAGATTGTGGATTTGTGAAAATGTGGAAGAGCTCTCGAAACCTATGTTTGACTATTGGAAGAAAAAATGTCTTGTGCAACAAAGGCAGTTAGATGATTCACTTGCCCAGGCAATGCAAGTGGCAAGCAGTCCAGAGGAGTGGAAATCACGAGGCATTAAGCTATATCATGAGCATAACTATGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGACACTTACTGGGAAAGGAGGTCTAAAGCTTCCGGCCTCAAAGCTATTGCTGACCGTATGCGAACATCAAATCCCGAAGAGGCTAAATCGATCCTTAGAGAAGCTGCTGAAATATTCGATGCTATTGGCAAGGCAGATTCTGCTGCGAGATGTTTTTCTGATTTGGGGGAGTATGAAAGAGCAGCTAGGATATATTTGGAAAAATGTGGAGACTCGGAACTTCAAAGAGCTGCAGAATGTTTTTCACTAGCGGGATGCTTTGAGGATGCTGCAGATGTCTATGCCAAGGGAAATTTTTTCGCAGAGTGTCTCTCTGTTTGTGCAAATGGGAAACTATTTGAAATGGGTTTGCAGTATATTAATTATTGGAAACAGCATGCTGTTGAGGACTGTGCTAAGGCTAGTAGAGGAGAAGGAATATATAAAATGGAGCAGGAATTTTTAGAGAGCTGCGCACTGCACTATCATAAGTTGAAAGATAACAGATTAATGATGAAATTTGTGAAAGCTTTCAATTCTATAATATTGATGCGGGACTTTTTGAAGAGTATAGGGAGCCTTGATGAGCTTTTGTCATTGGAGGAAGAATTTGGAAATTATCTGGAAGCCGCAAACATCGCCCAGCTTAAAGGTGAAATCCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAGGCCTCACTGCATATTCTGTTTTATGTAATTTCCAAGTCTCTTTGGTCATCTGGTGGCAAGGGCTGGCCCATGGAAAAGTTTCCACAACAGGGGGAGCTTCGGGAGCTTCTGACAAAGGCCAAGTCATTTGCCAAGATTGGGAAAGATAACGTTTATGAGCTGGTTTGCGCCGAGTCGGAGATTTTATTGAATGGGCAGGATAACTTGGCTGTTTTGAAACATCAAATGGATGCTTCTGAAAGACATCAGAGTATTAGAGGTGAAGTGTTATCAGCTCGAAAAATTTTGGACGCCCATCTCTCTCCAAGAACTAATAAGTATTTATGGGAGAAAGAATTAATTGGAGATCTTGTAAAGCATTCAAAAGATAAAATATCTAGAAATCAGGTGTCCATTGATTCACTTGTCTACTACTGGAATTTCTGGAAGGAGAAGATCCTTCACATGATTGAATCTGTAGGATGTGTGGAAACTCCAGAGTACAATAGTTATGTAGAACTCTGTTTTACTTGTTTAGGAGTTTGGAGGTTGTTTCATAATCTGAATCCAATCTATGTTTTACTGGTCCCTGATGCTGATTGGGTTAGAGGTCTGGACAAAAGGCATTTGAAAAGTTATGGGAAGTTGGTCTCGATTGATGTTCATCAGCTCATTCCAGCAGCTCAGTATTATTGGAGTTCAGAACTGCTCTCTGTTGGCATCAAAGTTTTGGAAAAGCTTGAAGCACTTTTCAAGTTCCCACTAAAGAATTCTGACTCGACATTCTTCCAGTGCAGGGCTCTTACCCTTATCTATGATGTTGCAAGAAATCTTTTGGATTCCAATTTGGATTCCAAATGTCTGAAGTCAAGGCATCAAGATTACTTGACACTACAGAAGCTGGTAACATTTTCGACTGGGGAATATGTTGCTTGCATCTTTCCTTTAGATTGGAGGAATTCGTTGAGACAGAATATGATTTCTCTTAGAAGAACTGATGCTTCAAAGAATGTGCTGAATCAAGTTATAGTTGAATATACCAGCTCGAAGAACAAGCTGTCTTATGGGCAGATTGGAAGGATCGCAATGATCATTCTCGGGTCAGGTAAGCTGCTGAATACTGAGCTCTCTGTTCAGCTTGTGAAAAAGCTTGAAGTCAGTAAACCCTGGAAGGCCTTCATTGAGAAACTCTGTGCGAGCGTTGGACCTGGACATACCCGTCAGGAACCAATAGAGGTGTCTGTCATGTGGCAGTTACATGCGGCTTTGGTTGAAACTTATAGTGTCAACTGGTTGGAGGTTTCCGACTACATATCACCTGGTTGTTTCTTGTATCTTGTGGAGCGCCTTCTGTTTTCAGCAAGTTGCTTTCAAGGTTTTGTCATCACTACAGGTTCTTGCTTTATTGAGTGGCTGATATACCAGGATGAAGATACCAGCTTACGTTCCATAGTGACTGGTGGGCGTCCTTCTTTTGGAACCATTCTTCCATTTGTGATTAGTGTGGTTAGCGATTGTATTTTTAAGAAGAAGGATATGATTGAATGGATTGAGAAGTCTGGCAGAAATTGGAGGAAGTATTATCAGCCACTCATGTTGCGGTTGGTTACTGTCTTGTGTTTGATTAATTTGAACTTTGGCTCGTGTTTGGATATAATCCATGATTTGCTGGGGAGGAACTTTATCACCGAACTCCAACCACGGGGGTTTTATGATGCACTTAGGTGGAGGAGGAAACACAATTCTCTCAGTTTAAATGTGAATGTCCTTGCTGGAGCTCTCAAGAAGATTGATAATGCATTGGTGATTGCGAGTTTCGGGGTTAATTGTTCAGGGTTTTACAGTTCGGATGCTATCTTTGTCGACATGACGACCAATCAATGCAAGGATGACATATTGAGAAAATGGTTTCCAAAGCCGCCTGTCATTCAAGTTGCACAACATCAAGTTGCATCGGTTGAAAAAGGTAGAAGTTCTTGCAGTCCTGCACTCTCTGCAACTGATTCTGAAGAAGGTAAGAGTTCTAAATCTGTGGCTTCAGAATCCAGCTTGGTAAAAGAGACTACTGTTGCAGCTGGTAAGCAGTGTGTAGCTGAAGATAGTCACAGCAAAGATGAGGGTAATTGCATATCCAATGCGTCAGATCAGCAGTGTCCAGGCCCAAGCTCGGAGCAGCATGACGCCGAGGACGGCCACACAAAAGGCGAGGAAAACAACGCATCCACCGAGTCAAATCTGACATGTCAAAAAGATGATGGTACTTCCACCCCATCTGGCAGTAAAGAGGCTGGAAACAAGGCGAAGAACAAGACAAAGGGCAAGAACAAGAAAAACAAGAAGAAAGGAAGCCGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2656

Amino Acids

302.25

Weight (kDa)

6.74

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 76 - 104 3.1e-09 Domain of unknown function (DUF6469)
DUF6469 PF20073 104 - 169 4e-24 Domain of unknown function (DUF6469)
AAA_11 PF13086 206 - 558 7e-25 AAA domain
AAA_12 PF13087 567 - 763 3.3e-54 AAA domain
UvrD-helicase PF00580 1274 - 1363 4.2e-08 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1901
AarI CACCTGC 1 cut(s) 4426
AasI GACNNNNNNGTC 2 cut(s) 950, 6831
Acc16I TGCGCA 1 cut(s) 5404
Acc36I ACCTGC 2 cut(s) 4426, 5609
AccB1I GGYRCC 1 cut(s) 3186
AccB7I CCANNNNNTGG 3 cut(s) 986, 1279, 2577
AccI GTMKAC 5 cut(s) 1193, 1263, 3934, 6042, 7476
AclI AACGTT 1 cut(s) 5784
AclWI GGATC 8 cut(s) 96, 1813, 2184, 2895, 3063, 5035, 6064, 6701
AcoI YGGCCR 3 cut(s) 3574, 4923, 7804
AcyI GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
AdeI CACNNNGTG 1 cut(s) 4932
AfeI AGCGCT 1 cut(s) 4652
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 4 cut(s) 492, 3325, 4032, 7853
AhdI GACNNNNNGTC 1 cut(s) 3294
AjuI GAANNNNNNNTTGG 6 cut(s) 3183, 3215, 3435, 3467, 4133, 4165
AleI CACNNNNGTG 1 cut(s) 7336
AloI GAACNNNNNNTCC 6 cut(s) 1052, 1084, 5005, 5037, 7084, 7116
Alw21I GWGCWC 9 cut(s) 226, 787, 1135, 2550, 4465, 4694, 4770, 6741, 7393
AlwI GGATC 8 cut(s) 96, 1813, 2184, 2895, 3063, 5035, 6064, 6701
AlwNI CAGNNNCTG 5 cut(s) 3137, 3257, 3569, 5090, 6514
Ama87I CYCGRG 2 cut(s) 183, 6710
Aor51HI AGCGCT 1 cut(s) 4652
ArsI GACNNNNNNTTYG 6 cut(s) 1995, 2027, 2840, 2872, 5741, 5773
AseI ATTAAT 5 cut(s) 2802, 5310, 5438, 5972, 7239
Asp700I GAANNNNTTC 6 cut(s) 554, 2507, 4209, 5459, 5712, 6788
AspLEI GCGC 5 cut(s) 3276, 4653, 5405, 5804, 6960
AsuC2I CCSGG 3 cut(s) 1520, 2878, 3812
AvaI CYCGRG 2 cut(s) 183, 6710
AvaII GGWCC 6 cut(s) 701, 1886, 2329, 3034, 6199, 6818
BaeI ACNNNNGTAYC 2 cut(s) 4265, 4298
BalI TGGCCA 2 cut(s) 3576, 4925
BanI GGYRCC 1 cut(s) 3186
BanII GRGCYC 4 cut(s) 4770, 6415, 6741, 7393
BarI GAAGNNNNNNTAC 2 cut(s) 3909, 3941
BauI CACGAG 2 cut(s) 4884, 7255
BbsI GAAGAC 1 cut(s) 2379
Bbv12I GWGCWC 9 cut(s) 226, 787, 1135, 2550, 4465, 4694, 4770, 6741, 7393
BceAI ACGGC 3 cut(s) 668, 1189, 7819
BcgI CGANNNNNNTGC 2 cut(s) 1551, 1585
BclI TGATCA 2 cut(s) 3961, 6702
BcnI CCSGG 3 cut(s) 1520, 2878, 3812
BfmI CTRYAG 9 cut(s) 755, 3211, 3249, 3753, 5181, 5220, 6090, 6506, 7002
BfoI RGCGCY 2 cut(s) 4654, 6961
BfrI CTTAAG 1 cut(s) 506
BfuAI ACCTGC 2 cut(s) 4426, 5609
BglII AGATCT 3 cut(s) 2715, 3997, 5980
BlpI GCTNAGC 1 cut(s) 138
Bme18I GGWCC 6 cut(s) 701, 1886, 2329, 3034, 6199, 6818
BmeRI GACNNNNNGTC 1 cut(s) 3294
BmeT110I CYCGRG 2 cut(s) 183, 6710
BmrI ACTGGG 4 cut(s) 642, 3147, 4969, 6540
BmuI ACTGGG 4 cut(s) 642, 3147, 4969, 6540
BoxI GACNNNNGTC 1 cut(s) 2525
BpiI GAAGAC 1 cut(s) 2379
BplI GAGNNNNNCTC 2 cut(s) 5246, 5278
BpmI CTGGAG 5 cut(s) 89, 2714, 4332, 6093, 7407
Bpu10I CCTNAGC 4 cut(s) 3300, 3745, 4647, 5346
Bpu1102I GCTNAGC 1 cut(s) 138
BpuEI CTTGAG 7 cut(s) 1046, 1119, 1311, 2046, 3923, 4553, 7379
BpuMI CCSGG 3 cut(s) 1520, 2878, 3812
Bsa29I ATCGAT 1 cut(s) 5039
BsaBI GATNNNNATC 2 cut(s) 2832, 6276
BsaHI GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
BsaI GGTCTC 2 cut(s) 2575, 6268
BsaWI WCCGGW 1 cut(s) 1293
BsaXI ACNNNNNCTCC 4 cut(s) 985, 1015, 5970, 6000
Bse3DI GCAATG 6 cut(s) 1599, 1884, 2290, 2679, 4854, 6705
Bse8I GATNNNNATC 2 cut(s) 2832, 6276
BseCI ATCGAT 1 cut(s) 5039
BseJI GATNNNNATC 2 cut(s) 2832, 6276
BseMI GCAATG 6 cut(s) 1599, 1884, 2290, 2679, 4854, 6705
BseRI GAGGAG 5 cut(s) 1937, 3431, 4886, 5373, 7357
BseYI CCCAGC 2 cut(s) 5571, 7282
BsgI GTGCAG 5 cut(s) 1614, 4449, 5393, 6427, 7584
BshNI GGYRCC 1 cut(s) 3186
BshVI ATCGAT 1 cut(s) 5039
BsiHKAI GWGCWC 9 cut(s) 226, 787, 1135, 2550, 4465, 4694, 4770, 6741, 7393
BsiHKCI CYCGRG 2 cut(s) 183, 6710
BsiSI CCGG 7 cut(s) 12, 1294, 1519, 2878, 3811, 4983, 7963
BslFI GGGAC 6 cut(s) 2093, 2735, 3138, 3535, 5498, 6185
BsmBI CGTCTC 1 cut(s) 4204
BsmFI GGGAC 6 cut(s) 2093, 2735, 3138, 3535, 5498, 6185
BsmI GAATGC 4 cut(s) 196, 1124, 1773, 5992
Bso31I GGTCTC 2 cut(s) 2575, 6268
BsoBI CYCGRG 2 cut(s) 183, 6710
Bsp1407I TGTACA 1 cut(s) 1998
Bsp1720I GCTNAGC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 5703
BspDI ATCGAT 1 cut(s) 5039
BspHI TCATGA 3 cut(s) 3454, 4386, 4903
BspMAI CTGCAG 3 cut(s) 3253, 5185, 5224
BspMI ACCTGC 2 cut(s) 4426, 5609
BspPI GGATC 8 cut(s) 96, 1813, 2184, 2895, 3063, 5035, 6064, 6701
BspQI GCTCTTC 1 cut(s) 4758
BspT107I GGYRCC 1 cut(s) 3186
BspTI CTTAAG 1 cut(s) 506
BspTNI GGTCTC 2 cut(s) 2575, 6268
BsrDI GCAATG 6 cut(s) 1599, 1884, 2290, 2679, 4854, 6705
BsrGI TGTACA 1 cut(s) 1998
BssNI GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
BssSI CACGAG 2 cut(s) 4884, 7255
BssT1I CCWWGG 6 cut(s) 980, 2325, 2388, 5235, 5608, 5703
Bst2BI CACGAG 2 cut(s) 4884, 7255
Bst6I CTCTTC 9 cut(s) 396, 1120, 1912, 2154, 3075, 4044, 4758, 5022, 5489
BstACI GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 3 cut(s) 230, 5090, 7607
BstAUI TGTACA 1 cut(s) 1998
BstDSI CCRYGG 3 cut(s) 684, 5703, 7313
BstENI CCTNNNNNAGG 1 cut(s) 5612
BstH2I RGCGCY 2 cut(s) 4654, 6961
BstHHI GCGC 5 cut(s) 3276, 4653, 5405, 5804, 6960
BstNSI RCATGY 8 cut(s) 496, 3329, 3354, 3765, 4036, 5330, 6875, 7857
BstPAI GACNNNNGTC 1 cut(s) 2525
BstSFI CTRYAG 9 cut(s) 755, 3211, 3249, 3753, 5181, 5220, 6090, 6506, 7002
BstV2I GAAGAC 1 cut(s) 2379
BstX2I RGATCY 7 cut(s) 88, 2715, 2887, 3055, 3997, 5980, 6069
BstXI CCANNNNNNTGG 2 cut(s) 2853, 4875
BstYI RGATCY 7 cut(s) 88, 2715, 2887, 3055, 3997, 5980, 6069
Bsu15I ATCGAT 1 cut(s) 5039
BsuTUI ATCGAT 1 cut(s) 5039
BtgI CCRYGG 3 cut(s) 684, 5703, 7313
BtgZI GCGATG 1 cut(s) 5551
BtsI GCAGTG 6 cut(s) 645, 1557, 5405, 5639, 7703, 7769
BveI ACCTGC 2 cut(s) 4426, 5609
CaiI CAGNNNCTG 5 cut(s) 3137, 3257, 3569, 5090, 6514
CciI TCATGA 3 cut(s) 3454, 4386, 4903
CfoI GCGC 5 cut(s) 3276, 4653, 5405, 5804, 6960
ClaI ATCGAT 1 cut(s) 5039
CpoI CGGWCCG 1 cut(s) 3034
CseI GACGC 6 cut(s) 1179, 4183, 5933, 7067, 7740, 7802
CsiI ACCWGGT 1 cut(s) 6931
CspCI CAANNNNNGTGG 4 cut(s) 4836, 4871, 7191, 7226
CspI CGGWCCG 1 cut(s) 3034
DraI TTTAAA 5 cut(s) 385, 1752, 4027, 4314, 7368
DraIII CACNNNGTG 1 cut(s) 4932
DrdI GACNNNNNNGTC 2 cut(s) 950, 6831
DriI GACNNNNNGTC 1 cut(s) 3294
DseDI GACNNNNNNGTC 2 cut(s) 950, 6831
EaeI YGGCCR 3 cut(s) 3574, 4923, 7804
Eam1104I CTCTTC 9 cut(s) 396, 1120, 1912, 2154, 3075, 4044, 4758, 5022, 5489
Eam1105I GACNNNNNGTC 1 cut(s) 3294
EarI CTCTTC 9 cut(s) 396, 1120, 1912, 2154, 3075, 4044, 4758, 5022, 5489
EciI GGCGGA 1 cut(s) 3372
Ecl136II GAGCTC 3 cut(s) 4768, 6739, 7391
Eco130I CCWWGG 6 cut(s) 980, 2325, 2388, 5235, 5608, 5703
Eco147I AGGCCT 3 cut(s) 2324, 5636, 6788
Eco24I GRGCYC 4 cut(s) 4770, 6415, 6741, 7393
Eco31I GGTCTC 2 cut(s) 2575, 6268
Eco32I GATATC 2 cut(s) 2743, 3439
Eco47I GGWCC 6 cut(s) 701, 1886, 2329, 3034, 6199, 6818
Eco47III AGCGCT 1 cut(s) 4652
Eco53kI GAGCTC 3 cut(s) 4768, 6739, 7391
Eco88I CYCGRG 2 cut(s) 183, 6710
EcoICRI GAGCTC 3 cut(s) 4768, 6739, 7391
EcoNI CCTNNNNNAGG 1 cut(s) 5612
EcoO109I RGGNCCY 2 cut(s) 701, 4528
EcoRI GAATTC 5 cut(s) 2213, 2956, 3896, 6382, 6571
EcoRV GATATC 2 cut(s) 2743, 3439
EcoT14I CCWWGG 6 cut(s) 980, 2325, 2388, 5235, 5608, 5703
EcoT22I ATGCAT 3 cut(s) 1773, 3763, 7414
EcoT38I GRGCYC 4 cut(s) 4770, 6415, 6741, 7393
ErhI CCWWGG 6 cut(s) 980, 2325, 2388, 5235, 5608, 5703
Esp3I CGTCTC 1 cut(s) 4204
FalI AAGNNNNNCTT 2 cut(s) 4614, 4646
FaqI GGGAC 6 cut(s) 2093, 2735, 3138, 3535, 5498, 6185
FauI CCCGC 2 cut(s) 5194, 5475
FauNDI CATATG 1 cut(s) 265
FbaI TGATCA 2 cut(s) 3961, 6702
FblI GTMKAC 5 cut(s) 1193, 1263, 3934, 6042, 7476
FriOI GRGCYC 4 cut(s) 4770, 6415, 6741, 7393
FspI TGCGCA 1 cut(s) 5404
GlaI GCGC 5 cut(s) 3275, 4652, 5404, 5803, 6959
GsaI CCCAGC 2 cut(s) 5575, 7286
GsuI CTGGAG 5 cut(s) 89, 2714, 4332, 6093, 7407
HaeII RGCGCY 2 cut(s) 4654, 6961
HapII CCGG 7 cut(s) 12, 1294, 1519, 2878, 3811, 4983, 7963
HgaI GACGC 6 cut(s) 1179, 4183, 5933, 7067, 7740, 7802
HhaI GCGC 5 cut(s) 3276, 4653, 5405, 5804, 6960
Hin1I GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
Hin6I GCGC 5 cut(s) 3274, 4651, 5403, 5802, 6958
HinP1I GCGC 5 cut(s) 3274, 4651, 5403, 5802, 6958
HincII GTYRAC 4 cut(s) 3935, 4218, 6901, 7477
HindII GTYRAC 4 cut(s) 3935, 4218, 6901, 7477
HindIII AAGCTT 7 cut(s) 1705, 2564, 3692, 4977, 5457, 6350, 6761
HpaII CCGG 7 cut(s) 12, 1294, 1519, 2878, 3811, 4983, 7963
Hpy99I CGWCG 3 cut(s) 3936, 4199, 4470
HpyCH4IV ACGT 4 cut(s) 2700, 3699, 5784, 7057
HpySE526I ACGT 4 cut(s) 2700, 3699, 5784, 7057
Hsp92I GRCGYC 4 cut(s) 4194, 5925, 7078, 7794
HspAI GCGC 5 cut(s) 3274, 4651, 5403, 5802, 6958
Ksp22I TGATCA 2 cut(s) 3961, 6702
LguI GCTCTTC 1 cut(s) 4758
MabI ACCWGGT 1 cut(s) 6931
MaeII ACGT 4 cut(s) 2700, 3699, 5784, 7057
MflI RGATCY 7 cut(s) 88, 2715, 2887, 3055, 3997, 5980, 6069
MlsI TGGCCA 2 cut(s) 3576, 4925
MluNI TGGCCA 2 cut(s) 3576, 4925
Mox20I TGGCCA 2 cut(s) 3576, 4925
Mph1103I ATGCAT 3 cut(s) 1773, 3763, 7414
MroXI GAANNNNTTC 6 cut(s) 554, 2507, 4209, 5459, 5712, 6788
MscI TGGCCA 2 cut(s) 3576, 4925
MslI CAYNNNNRTG 4 cut(s) 1223, 1374, 4914, 7336
Msp20I TGGCCA 2 cut(s) 3576, 4925
MspA1I CMGCKG 1 cut(s) 7688
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 7 cut(s) 12, 1294, 1519, 2878, 3811, 4983, 7963
MssI GTTTAAAC 1 cut(s) 4027
Mva1269I GAATGC 4 cut(s) 196, 1124, 1773, 5992
NciI CCSGG 3 cut(s) 1520, 2878, 3812
NcoI CCATGG 1 cut(s) 5703
NdeI CATATG 1 cut(s) 265
NmeAIII GCCGAG 2 cut(s) 5830, 7822
NmuCI GTSAC 7 cut(s) 63, 2856, 3286, 3559, 4949, 7066, 7715
NsbI TGCGCA 1 cut(s) 5404
NsiI ATGCAT 3 cut(s) 1773, 3763, 7414
NspI RCATGY 8 cut(s) 496, 3329, 3354, 3765, 4036, 5330, 6875, 7857
OliI CACNNNNGTG 1 cut(s) 7336
PaeI GCATGC 2 cut(s) 3354, 5330
PagI TCATGA 3 cut(s) 3454, 4386, 4903
PaqCI CACCTGC 1 cut(s) 4426
PceI AGGCCT 3 cut(s) 2324, 5636, 6788
PciI ACATGT 4 cut(s) 492, 3325, 4032, 7853
PciSI GCTCTTC 1 cut(s) 4758
PcsI WCGNNNNNNNCGW 1 cut(s) 7836
PctI GAATGC 4 cut(s) 196, 1124, 1773, 5992
PdmI GAANNNNTTC 6 cut(s) 554, 2507, 4209, 5459, 5712, 6788
PflFI GACNNNGTC 1 cut(s) 1944
PflMI CCANNNNNTGG 3 cut(s) 986, 1279, 2577
PfoI TCCNGGA 3 cut(s) 1518, 1821, 1870
PmeI GTTTAAAC 1 cut(s) 4027
PpuMI RGGWCCY 1 cut(s) 701
PscI ACATGT 4 cut(s) 492, 3325, 4032, 7853
PshAI GACNNNNGTC 1 cut(s) 2525
PshBI ATTAAT 5 cut(s) 2802, 5310, 5438, 5972, 7239
PsiI TTATAA 1 cut(s) 1901
Psp124BI GAGCTC 3 cut(s) 4770, 6741, 7393
Psp1406I AACGTT 1 cut(s) 5784
Psp5II RGGWCCY 1 cut(s) 701
PspFI CCCAGC 2 cut(s) 5571, 7282
PspPPI RGGWCCY 1 cut(s) 701
PsrI GAACNNNNNNTAC 4 cut(s) 2681, 2713, 4611, 4643
PstI CTGCAG 3 cut(s) 3253, 5185, 5224
PstNI CAGNNNCTG 5 cut(s) 3137, 3257, 3569, 5090, 6514
PsuI RGATCY 7 cut(s) 88, 2715, 2887, 3055, 3997, 5980, 6069
PsyI GACNNNGTC 1 cut(s) 1944
PvuII CAGCTG 1 cut(s) 7688
RseI CAYNNNNRTG 4 cut(s) 1223, 1374, 4914, 7336
Rsr2I CGGWCCG 1 cut(s) 3034
RsrII CGGWCCG 1 cut(s) 3034
SacI GAGCTC 3 cut(s) 4770, 6741, 7393
SalI GTCGAC 2 cut(s) 3933, 7475
SapI GCTCTTC 1 cut(s) 4758
SexAI ACCWGGT 1 cut(s) 6931
SfcI CTRYAG 9 cut(s) 755, 3211, 3249, 3753, 5181, 5220, 6090, 6506, 7002
SinI GGWCC 6 cut(s) 701, 1886, 2329, 3034, 6199, 6818
SmiMI CAYNNNNRTG 4 cut(s) 1223, 1374, 4914, 7336
SmlI CTYRAG 8 cut(s) 506, 1025, 1134, 1326, 2061, 3938, 4532, 7394
SmoI CTYRAG 8 cut(s) 506, 1025, 1134, 1326, 2061, 3938, 4532, 7394
SphI GCATGC 2 cut(s) 3354, 5330
SseBI AGGCCT 3 cut(s) 2324, 5636, 6788
SspI AATATT 3 cut(s) 1498, 5064, 5475
SstI GAGCTC 3 cut(s) 4770, 6741, 7393
StuI AGGCCT 3 cut(s) 2324, 5636, 6788
StyI CCWWGG 6 cut(s) 980, 2325, 2388, 5235, 5608, 5703
TaiI ACGT 4 cut(s) 2703, 3702, 5787, 7060
TatI WGTACW 2 cut(s) 1998, 6114
TauI GCSGC 4 cut(s) 3357, 5561, 6878, 7534
TseFI GTSAC 7 cut(s) 63, 2856, 3286, 3559, 4949, 7066, 7715
Tsp45I GTSAC 7 cut(s) 63, 2856, 3286, 3559, 4949, 7066, 7715
TspGWI ACGGA 1 cut(s) 4220
Tth111I GACNNNGTC 1 cut(s) 1944
Van91I CCANNNNNTGG 3 cut(s) 986, 1279, 2577
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 6 cut(s) 701, 1886, 2329, 3034, 6199, 6818
VspI ATTAAT 5 cut(s) 2802, 5310, 5438, 5972, 7239
XagI CCTNNNNNAGG 1 cut(s) 5612
XbaI TCTAGA 3 cut(s) 2734, 2836, 6011
XceI RCATGY 8 cut(s) 496, 3329, 3354, 3765, 4036, 5330, 6875, 7857
XcmI CCANNNNNNNNNTGG 1 cut(s) 987
XmiI GTMKAC 5 cut(s) 1193, 1263, 3934, 6042, 7476
XmnI GAANNNNTTC 6 cut(s) 554, 2507, 4209, 5459, 5712, 6788
Zsp2I ATGCAT 3 cut(s) 1773, 3763, 7414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.