Rh4DG436000

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000223
Physical Location & Seq
Forward (+)
63930480 .. 63935794
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG436000.1

Sequence Viewer

Length: 3975 bp
ATGAGCTGGCCAGCCTCCTTGGATGTAATCCGATATAAGGATCTTAGCGTCACTAGTGATTCAGTGGATGACGTGTCTAAGGATAAAAGTTATGTTGAAAATTCAAAGGTCAGTGAGAGTTTTTTGCTGATGAAATTCTACTCCTTATCATCTGGGGTGGTGAATCACTTGCTCTCTGACCGTGAGGGTAGAGAGTTGGTTCTCCCATTTGAAGTTACTGATGAAGAAATGGAGGCCATACTTTACAATAGAAGTGCCTTCATAGTTGGGCGATCAGGCACTGGGAAGACCACTGTTTTAACAATGAAGTTATATCAGAAGGAACAGTATTACAATATGGCAAAGGAAGGATTGTATGCTGTCGTGCAGAGTTCTGGAGGTTCTAATGCAACTGTTTTACGCCAGATATTTGTAACAGTTAGTCCAAAACTCTGTTTTGCCATAAAGCAACATGTATCGAACTTGAGAAGTTTTGCTTCTGGTGTAAGCTGCCATTCAGCTGAATTCGGCTCGATTGGTCTGGATGATTATGATGATGCAGAAGCAGAATTTAGGGATCTCCCAAATTCATTTCTTGATATTCCTTCCGAGCGTTATCCTCTTATTATAACATTCCATAAATTTTTAATGATGCTGGATGGAAGTTTGAGTAATTCATACTTTGAAAGATTCCTTGACAACCTAAAACTGCCTCGAAGGCCACCGCAAAGTTCAAGATCGCTGAAAAGCTTATTGAGGACAAAGGAGGTCAATTACGAAAGGTTTTGTTCATCATATTGGCCTCATTTTAATATGAAGTTAACGAAGCGGCTTGATGCCTTGAGAGTCATTACTGAGATTATTTCTCATATCAAAGGTGGTCTTAGAGCTTTGGAAGCAGGTAATGGAAAACTCAGTCAGTCGGATTATGTGAAAATGTCTGAGTGCCGGGCTTCCAATTTATGCCAGAAAGAGAGAGAGATAATATATGATATTTTTCAGGTATATGAGAAAATGAAGACAAGAAATGGTGAATATGATATTGCTGATTTTGTAATTGATATTCACCGTCGACTCCAACGTGAGAAATATAAGGGTGATGAAATTAATTTTGTGTACATCGATGAGGTACAGGATCTAACGATGAGTCAAATCATGCTGTTCAAGCATATATGCAGTAATGTTGAGGAGGGTTTTGTTTTTTCGGGTGATACGGCCCAAACCATTTCAAAGGGTATTGATTTTAGATTCCAAGATATACGGCATCTGTTCTACAAGAAGTTTGTCTTGGATTCAAGAAGCAATGAGCATAACGAAAGGAATGACAAAGCAGAAATCTCAAAAATGTTTCATTTGTCTCAAAACTTCCGCACACATGGTGGCGTACTGAGTGCTACTAATTTTGTTGGGTTTGGAGCTGAGCAGGTCATCTTGGTGCGTGATGCTACCGTTCAGAAGGAGATTTCTAACTCTTTTGGGAAGCAAGCTCTTGTTCTTACTATTGTGGAGTGCAAGGGGCTCGAGTTCCAGGATGTACTTTTGTACAAGTATTTTGGGTCATCACCCTTAATAAATGAATGGAGGCTGATATATAATTACATGAAGGAACATGATTTGCTTAGTCCCACTTTACATAAGTCGATTCCAACATTTGACAGGGCAAAACACAATTTTTTATGCTCTGAACTGAAGCAATTATATGTTGCTATTTCACGTACAAGAAATAGATTGTGGATTTATGAGGACATGGAAGGTCTCTGGAATCCCATGTTTGACTATTGGATAAAGAAATGTCTTGTACAAGTGAGGCAACTTGATGATTCCCTTGTAGAAAAAATGAAAGTTTCAAGCAGTGCAGAGGAATGGAAATCGCGTGGCATGAAGCTGTATCATGAGCGTAATTATGAAATGGCCAATATGTGCTTCCAAAATGCACATGATACTTACTGGGAAAGAAGGTCAAAAGCCGGCAGTCTCAAAGTTATGGCTTATTGTATGCAAACATCAAATCCTGAAAAGGCTAAATCCATTCTTAGGGAAGCTGCTGAAATTTTTGAAGATATAGGGATGGCAAATTCTGCTGCCCAATGCTTTTTTGATTCAGGGGATTATGATGTAGCAGGTAGGATTTATTACCACAAATGTGGTAAACAAGCACGTGAAAGAGCTGCGGAATGCTTCTTTCTAGCAGAGCGCTATGAATCTGCTGCAGAAATATTTCAATCCATAGGCATGGCAGAGTCTGCTGCAAGATGCTATTACGAATTGGGGCAGTATGAAAGGGCAGGGACTATTTATTTGGAAGACTGTGGTGACCCTGGACTGGAAAGAGCTGGGGAGTGTTTCTATCTAGCAGGATGCTATAAGGGTGCAGCTGATGCCTATGGTAGGGGAAATTTTTTATCCGATTGTCTTAGTGTGTGTTTAAAGGGAAAGCTGTTTGACATAGGTTTGAAATATATTAAAAGTTGGAAGCAACACGCAAGAAAAGAGCATGATCTGGCTATAAGAGGAGAACTGGAGTTTCTTGAGAGCTGTGCATTTCACTATTATGAGGTTGAAGATACAAGATCCAACATGAAATCTGTTAGAAGTGAAATGTTGTCCAGTCGAAAGATTTTAAATGCTCATCTCTCTTCTAGTCCAAAAATATATTTGTGGGATGACAAACTGATTCGTGATTTTCGTGATCTGAAAATCTATTTGAAAGTCAAACAATCTGAAAAGCAGTTTTCTGTAGATTCACTGGTGTACTTCTGGAATGTTTGGAAGGATATGGTAGTTTACTTGATTGAATATTTTGAATCTGTTGAAACCCAAGCTGTTAATAGAAAGAGAACTTACGGAGACTTCTTTCTGAATTACCTGGGAGTGTGGAGGCAGTTTCATGATGATCTGTGTTTTGTCTACTCTGTACTGATCTCTGATGCAAATTGGGTAAGAGGAATGGACAAAAGGTCTTTTCAAATTAACGGGGAGCTGGTCACCATAGATGTTTGGCAGATAATTTCCGCCGCTCATAGCTATTGGACTTCAGAACTTCTTTCAGTTGGCATTAGGATTTTGAATAAGGTTGAAGCGCTTTACAGGGTCAAATCTGATTCAGTTTTCTGCCAAAGCTGGTGTCTTACGCAGATTTATGTTGTTGCGAAGTGTCTCTTGGAATCCAAATATCTGAAGCTAAGGAACGAAGATTCCTATACACTACAGAATTTTGTTGAACTGTCATCTGAAGATATTGTTGCTTGTATATTTCCTCCTGATTGGAGGAAATCTGTAAGAGAGAACATGATTACTCATAGACAAAAGGATACTTCTAAAAGTTTGCTCAAACAAGTTGTAGTTGATTTATTTACCAGCTCAAAGAAAAATCTTTCTTATGGCCAAGTTGGAAGGCTGGCAATGATTATTCTTGGGTCTGGTAATTTTGATGATGATGAACTATACAAGAAACTTATGAAAAATCTGAAATGTAGTCCACTGTGGAAGGTGTTCATTCAGAATCTGTGTGGGAGTATCATCGCAAAACAGAAAGGGTATGTACTTATCAATACCAGTCAAGAAAAGAAAGATGTGTCTCTTGTCCAGGGCCTTCATCAGGCTTTGGCAGAAACTTATTATGCAAACTGGAGGGAGGTTAATGACTATATTTCACCTGATGGTTTCTTTTATCTTATTGAATGCCTTTTGATGTGGGTATCTTGCTTTCAAGGTTTTGTTATCACCACCAAATCATGTTTCATTGAATGGTTGATGCAGAAGGAAGATACCAAACTCACTGCCAGTGTACTGGCTGATGTGCTAATATCTTTTGAACCCATCCTTATGTTTCTGATCGATGTGGTTCGGGAGGTTCTTTATGATAAGAAGACTATGGTTGAATGGATTCAAAGGTCTATCTCACAGTGGGAGGAGTGTCATTCGCTGCTGATCCTGAGATCGGTTGTTATCTTGTGTTTGCTTTATTTGAATTTTGGAATGGGGTATGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1324

Amino Acids

153.51

Weight (kDa)

6.57

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 313 - 402 2.3e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 608
Acc36I ACCTGC 3 cut(s) 869, 1393, 2090
AccBSI CCGCTC 1 cut(s) 2996
AccI GTMKAC 2 cut(s) 1051, 2886
AccII CGCG 1 cut(s) 1852
AciI CCGC 6 cut(s) 704, 808, 1348, 2150, 2991, 2994
AclWI GGATC 5 cut(s) 48, 564, 1122, 2544, 3904
AcoI YGGCCR 3 cut(s) 8, 1890, 3361
AcuI CTGAAG 4 cut(s) 1688, 2997, 3176, 3231
AcvI CACGTG 1 cut(s) 2138
AdeI CACNNNGTG 1 cut(s) 1358
AfeI AGCGCT 2 cut(s) 2174, 3060
AfiI CCNNNNNNNGG 6 cut(s) 37, 2013, 2302, 2367, 3577, 3919
AflIII ACRYGT 2 cut(s) 72, 451
AhdI GACNNNNNGTC 1 cut(s) 2935
AhlI ACTAGT 1 cut(s) 53
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 1506, 2296, 2844, 3564
AjuI GAANNNNNNNTTGG 8 cut(s) 1250, 1282, 1886, 1918, 3122, 3154, 3701, 3733
AleI CACNNNNGTG 1 cut(s) 2121
AloI GAACNNNNNNTCC 2 cut(s) 3158, 3190
Alw26I GTCTC 6 cut(s) 1341, 1739, 1958, 2820, 3140, 3561
AlwI GGATC 5 cut(s) 48, 564, 1122, 2544, 3904
AlwNI CAGNNNCTG 3 cut(s) 281, 2222, 3484
Ama87I CYCGRG 1 cut(s) 1499
Aor51HI AGCGCT 2 cut(s) 2174, 3060
AoxI GGCC 8 cut(s) 8, 234, 698, 779, 1194, 1890, 3361, 3568
ApeKI GCWGC 8 cut(s) 489, 2021, 2060, 2147, 2186, 2225, 2351, 3904
ArsI GACNNNNNNTTYG 4 cut(s) 2260, 2292, 3088, 3120
AseI ATTAAT 1 cut(s) 1086
Asp700I GAANNNNTTC 3 cut(s) 2196, 3470, 3864
AspLEI GCGC 2 cut(s) 2175, 3061
AspS9I GGNCC 2 cut(s) 1195, 3568
AsuC2I CCSGG 1 cut(s) 929
AvaI CYCGRG 1 cut(s) 1499
BalI TGGCCA 3 cut(s) 10, 1892, 3363
BanII GRGCYC 1 cut(s) 1500
BbrPI CACGTG 1 cut(s) 2138
BbsI GAAGAC 4 cut(s) 293, 1004, 2289, 3854
BbvI GCAGC 8 cut(s) 476, 2008, 2047, 2134, 2173, 2212, 2363, 3891
BccI CCATC 4 cut(s) 632, 2041, 3632, 3806
BceAI ACGGC 2 cut(s) 1209, 1256
BcgI CGANNNNNNTGC 2 cut(s) 1480, 1514
BciT130I CCWGG 4 cut(s) 1508, 2298, 2846, 3566
BciVI GTATCC 1 cut(s) 3283
BcnI CCSGG 1 cut(s) 929
BcoDI GTCTC 6 cut(s) 1341, 1739, 1958, 2820, 3140, 3561
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 4 cut(s) 54, 2165, 2330, 2619
BfmI CTRYAG 3 cut(s) 2187, 2715, 3185
BfoI RGCGCY 2 cut(s) 2176, 3062
BfuAI ACCTGC 3 cut(s) 869, 1393, 2090
BfuI GTATCC 1 cut(s) 3283
BglI GCCNNNNNGGC 1 cut(s) 697
BlpI GCTNAGC 1 cut(s) 1398
Bme1390I CCNGG 5 cut(s) 929, 1508, 2298, 2846, 3566
BmeRI GACNNNNNGTC 1 cut(s) 2935
BmeT110I CYCGRG 1 cut(s) 1499
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 1195, 3568
BmrFI CCNGG 5 cut(s) 929, 1508, 2298, 2846, 3566
BmrI ACTGGG 2 cut(s) 291, 1936
BmuI ACTGGG 2 cut(s) 291, 1936
BpiI GAAGAC 4 cut(s) 293, 1004, 2289, 3854
BpmI CTGGAG 3 cut(s) 396, 2519, 3628
Bpu10I CCTNAGC 1 cut(s) 3161
Bpu1102I GCTNAGC 1 cut(s) 1398
BpuEI CTTGAG 3 cut(s) 484, 841, 2528
BpuMI CCSGG 1 cut(s) 929
Bsa29I ATCGAT 2 cut(s) 1101, 3816
BsaAI YACGTR 2 cut(s) 1694, 2138
BsaI GGTCTC 1 cut(s) 1739
BsaJI CCNNGG 4 cut(s) 18, 2296, 2845, 3565
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 2013, 2302, 2367, 3577, 3919
Bse118I RCCGGY 1 cut(s) 1946
Bse3DI GCAATG 2 cut(s) 1288, 3387
BseBI CCWGG 4 cut(s) 1508, 2298, 2846, 3566
BseCI ATCGAT 2 cut(s) 1101, 3816
BseDI CCNNGG 4 cut(s) 18, 2296, 2845, 3565
BseGI GGATG 9 cut(s) 28, 73, 529, 643, 1516, 2052, 2342, 2647, 3798
BseLI CCNNNNNNNGG 6 cut(s) 37, 2013, 2302, 2367, 3577, 3919
BseMI GCAATG 2 cut(s) 1288, 3387
BseMII CTCAG 6 cut(s) 825, 907, 912, 1358, 1389, 3905
BseRI GAGGAG 3 cut(s) 1181, 2505, 3905
BseXI GCAGC 8 cut(s) 476, 2008, 2047, 2134, 2173, 2212, 2363, 3891
BseYI CCCAGC 1 cut(s) 2312
BsgI GTGCAG 3 cut(s) 386, 1854, 2370
Bsh1236I CGCG 1 cut(s) 1852
BshFI GGCC 8 cut(s) 10, 236, 700, 781, 1196, 1892, 3363, 3570
BshVI ATCGAT 2 cut(s) 1101, 3816
BsiHKCI CYCGRG 1 cut(s) 1499
BsiSI CCGG 2 cut(s) 928, 1947
BslFI GGGAC 2 cut(s) 1587, 2281
BslI CCNNNNNNNGG 6 cut(s) 37, 2013, 2302, 2367, 3577, 3919
BsmAI GTCTC 6 cut(s) 1341, 1739, 1958, 2820, 3140, 3561
BsmFI GGGAC 2 cut(s) 1587, 2281
BsmI GAATGC 2 cut(s) 2159, 3665
BsnI GGCC 8 cut(s) 10, 236, 700, 781, 1196, 1892, 3363, 3570
Bso31I GGTCTC 1 cut(s) 1739
BsoBI CYCGRG 1 cut(s) 1499
Bsp1286I GDGCHC 1 cut(s) 1500
Bsp1407I TGTACA 3 cut(s) 1095, 1521, 1777
Bsp1720I GCTNAGC 1 cut(s) 1398
BspACI CCGC 6 cut(s) 704, 808, 1348, 2150, 2991, 2994
BspANI GGCC 8 cut(s) 10, 236, 700, 781, 1196, 1892, 3363, 3570
BspCNI CTCAG 6 cut(s) 826, 906, 913, 1359, 1390, 3906
BspDI ATCGAT 2 cut(s) 1101, 3816
BspFNI CGCG 1 cut(s) 1852
BspHI TCATGA 2 cut(s) 1870, 2866
BspMAI CTGCAG 1 cut(s) 2191
BspMI ACCTGC 3 cut(s) 869, 1393, 2090
BspPI GGATC 5 cut(s) 48, 564, 1122, 2544, 3904
BspTNI GGTCTC 1 cut(s) 1739
BsrBI CCGCTC 1 cut(s) 2996
BsrDI GCAATG 2 cut(s) 1288, 3387
BsrFI RCCGGY 1 cut(s) 1946
BsrGI TGTACA 3 cut(s) 1095, 1521, 1777
BssAI RCCGGY 1 cut(s) 1946
BssECI CCNNGG 4 cut(s) 18, 2296, 2845, 3565
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 4 cut(s) 1508, 2298, 2846, 3566
Bst6I CTCTTC 1 cut(s) 2620
BstAPI GCANNNNNTGC 3 cut(s) 2057, 2222, 2357
BstAUI TGTACA 3 cut(s) 1095, 1521, 1777
BstBAI YACGTR 2 cut(s) 1694, 2138
BstC8I GCNNGC 5 cut(s) 8, 12, 1464, 1948, 3378
BstEII GGTNACC 2 cut(s) 2291, 2962
BstENI CCTNNNNNAGG 2 cut(s) 2365, 3575
BstF5I GGATG 9 cut(s) 28, 73, 529, 643, 1516, 2052, 2342, 2647, 3798
BstFNI CGCG 1 cut(s) 1852
BstH2I RGCGCY 2 cut(s) 2176, 3062
BstHHI GCGC 2 cut(s) 2175, 3061
BstMAI GTCTC 6 cut(s) 1341, 1739, 1958, 2820, 3140, 3561
BstMWI GCNNNNNNNGC 6 cut(s) 697, 875, 1144, 2057, 2222, 2357
BstNI CCWGG 4 cut(s) 1508, 2298, 2846, 3566
BstNSI RCATGY 1 cut(s) 455
BstPI GGTNACC 2 cut(s) 2291, 2962
BstSCI CCNGG 5 cut(s) 927, 1506, 2296, 2844, 3564
BstSFI CTRYAG 3 cut(s) 2187, 2715, 3185
BstUI CGCG 1 cut(s) 1852
BstV1I GCAGC 8 cut(s) 476, 2008, 2047, 2134, 2173, 2212, 2363, 3891
BstV2I GAAGAC 4 cut(s) 293, 1004, 2289, 3854
BstX2I RGATCY 4 cut(s) 40, 556, 1114, 2549
BstXI CCANNNNNNTGG 3 cut(s) 2123, 2212, 3769
BstYI RGATCY 4 cut(s) 40, 556, 1114, 2549
Bsu15I ATCGAT 2 cut(s) 1101, 3816
BsuI GTATCC 1 cut(s) 3283
BsuRI GGCC 8 cut(s) 10, 236, 700, 781, 1196, 1892, 3363, 3570
BsuTUI ATCGAT 2 cut(s) 1101, 3816
BtgZI GCGATG 1 cut(s) 3484
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 9 cut(s) 28, 73, 529, 643, 1516, 2052, 2342, 2647, 3798
BtsI GCAGTG 2 cut(s) 1837, 3756
BveI ACCTGC 3 cut(s) 869, 1393, 2090
Cac8I GCNNGC 5 cut(s) 8, 12, 1464, 1948, 3378
CaiI CAGNNNCTG 3 cut(s) 281, 2222, 3484
CciI TCATGA 2 cut(s) 1870, 2866
CfoI GCGC 2 cut(s) 2175, 3061
Cfr10I RCCGGY 1 cut(s) 1946
Cfr13I GGNCC 2 cut(s) 1195, 3568
ClaI ATCGAT 2 cut(s) 1101, 3816
CseI GACGC 1 cut(s) 37
DraI TTTAAA 2 cut(s) 2406, 2601
DraIII CACNNNGTG 1 cut(s) 1358
DriI GACNNNNNGTC 1 cut(s) 2935
EaeI YGGCCR 3 cut(s) 8, 1890, 3361
Eam1104I CTCTTC 1 cut(s) 2620
Eam1105I GACNNNNNGTC 1 cut(s) 2935
EarI CTCTTC 1 cut(s) 2620
EciI GGCGGA 1 cut(s) 2980
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 1500
Eco31I GGTCTC 1 cut(s) 1739
Eco47III AGCGCT 2 cut(s) 2174, 3060
Eco57I CTGAAG 4 cut(s) 1688, 2997, 3176, 3231
Eco72I CACGTG 1 cut(s) 2138
Eco88I CYCGRG 1 cut(s) 1499
Eco91I GGTNACC 2 cut(s) 2291, 2962
EcoNI CCTNNNNNAGG 2 cut(s) 2365, 3575
EcoO109I RGGNCCY 1 cut(s) 3568
EcoO65I GGTNACC 2 cut(s) 2291, 2962
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 4 cut(s) 1506, 2296, 2844, 3564
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 1500
ErhI CCWWGG 1 cut(s) 18
FalI AAGNNNNNCTT 8 cut(s) 460, 492, 846, 878, 1250, 1282, 3122, 3154
FaqI GGGAC 2 cut(s) 1587, 2281
FblI GTMKAC 2 cut(s) 1051, 2886
FokI GGATG 9 cut(s) 35, 80, 536, 650, 1523, 2059, 2349, 2654, 3785
FriOI GRGCYC 1 cut(s) 1500
FspBI CTAG 4 cut(s) 54, 2165, 2330, 2619
GlaI GCGC 2 cut(s) 2174, 3060
GsaI CCCAGC 1 cut(s) 2316
GsuI CTGGAG 3 cut(s) 396, 2519, 3628
HaeII RGCGCY 2 cut(s) 2176, 3062
HaeIII GGCC 8 cut(s) 10, 236, 700, 781, 1196, 1892, 3363, 3570
HapII CCGG 2 cut(s) 928, 1947
HgaI GACGC 1 cut(s) 37
HhaI GCGC 2 cut(s) 2175, 3061
Hin6I GCGC 2 cut(s) 2173, 3059
HinP1I GCGC 2 cut(s) 2173, 3059
HincII GTYRAC 2 cut(s) 801, 1052
HindII GTYRAC 2 cut(s) 801, 1052
HindIII AAGCTT 1 cut(s) 727
HpaI GTTAAC 1 cut(s) 801
HpaII CCGG 2 cut(s) 928, 1947
Hpy166II GTNNAC 9 cut(s) 801, 1052, 1096, 2129, 2731, 2764, 2887, 3458, 3767
Hpy8I GTNNAC 9 cut(s) 801, 1052, 1096, 2129, 2731, 2764, 2887, 3458, 3767
Hpy99I CGWCG 1 cut(s) 1053
HpyCH4IV ACGT 4 cut(s) 72, 1060, 1693, 2137
HpyF10VI GCNNNNNNNGC 6 cut(s) 697, 875, 1144, 2057, 2222, 2357
HpySE526I ACGT 4 cut(s) 72, 1060, 1693, 2137
HspAI GCGC 2 cut(s) 2173, 3059
KroI GCCGGC 1 cut(s) 1946
KroNI GCCGGC 1 cut(s) 1948
KspAI GTTAAC 1 cut(s) 801
LmnI GCTCC 2 cut(s) 1394, 2956
Lsp1109I GCAGC 8 cut(s) 476, 2008, 2047, 2134, 2173, 2212, 2363, 3891
MaeI CTAG 4 cut(s) 54, 2165, 2330, 2619
MaeII ACGT 4 cut(s) 72, 1060, 1693, 2137
MaeIII GTNAC 5 cut(s) 49, 214, 412, 2291, 2962
MbiI CCGCTC 1 cut(s) 2996
MflI RGATCY 4 cut(s) 40, 556, 1114, 2549
MhlI GDGCHC 1 cut(s) 1500
MlsI TGGCCA 3 cut(s) 10, 1892, 3363
MluNI TGGCCA 3 cut(s) 10, 1892, 3363
MlyI GAGTC 4 cut(s) 834, 1047, 1135, 2228
MmeI TCCRAC 6 cut(s) 882, 1081, 1649, 2429, 2577, 3349
Mox20I TGGCCA 3 cut(s) 10, 1892, 3363
MroNI GCCGGC 1 cut(s) 1946
MroXI GAANNNNTTC 3 cut(s) 2196, 3470, 3864
MscI TGGCCA 3 cut(s) 10, 1892, 3363
MslI CAYNNNNRTG 5 cut(s) 1412, 2121, 2210, 2529, 3803
Msp20I TGGCCA 3 cut(s) 10, 1892, 3363
MspA1I CMGCKG 2 cut(s) 500, 2354
MspI CCGG 2 cut(s) 928, 1947
MspR9I CCNGG 5 cut(s) 929, 1508, 2298, 2846, 3566
Mva1269I GAATGC 2 cut(s) 2159, 3665
MvaI CCWGG 4 cut(s) 1508, 2298, 2846, 3566
MvnI CGCG 1 cut(s) 1852
MwoI GCNNNNNNNGC 6 cut(s) 697, 875, 1144, 2057, 2222, 2357
NaeI GCCGGC 1 cut(s) 1948
NciI CCSGG 1 cut(s) 929
NgoMIV GCCGGC 1 cut(s) 1946
NmuCI GTSAC 3 cut(s) 49, 2291, 2962
NspI RCATGY 1 cut(s) 455
OliI CACNNNNGTG 1 cut(s) 2121
PaeR7I CTCGAG 1 cut(s) 1499
PagI TCATGA 2 cut(s) 1870, 2866
PciI ACATGT 1 cut(s) 451
PcsI WCGNNNNNNNCGW 2 cut(s) 1057, 2662
PctI GAATGC 2 cut(s) 2159, 3665
PdiI GCCGGC 1 cut(s) 1948
PdmI GAANNNNTTC 3 cut(s) 2196, 3470, 3864
PfoI TCCNGGA 1 cut(s) 1506
PleI GAGTC 4 cut(s) 833, 1047, 1134, 2227
PmaCI CACGTG 1 cut(s) 2138
PmlI CACGTG 1 cut(s) 2138
PpsI GAGTC 4 cut(s) 833, 1047, 1134, 2227
Ppu21I YACGTR 2 cut(s) 1694, 2138
PscI ACATGT 1 cut(s) 451
PshBI ATTAAT 1 cut(s) 1086
PsiI TTATAA 1 cut(s) 608
Psp6I CCWGG 4 cut(s) 1506, 2296, 2844, 3564
PspCI CACGTG 1 cut(s) 2138
PspEI GGTNACC 2 cut(s) 2291, 2962
PspFI CCCAGC 1 cut(s) 2312
PspGI CCWGG 4 cut(s) 1506, 2296, 2844, 3564
PspPI GGNCC 2 cut(s) 1195, 3568
PspXI VCTCGAGB 1 cut(s) 1499
PstI CTGCAG 1 cut(s) 2191
PstNI CAGNNNCTG 3 cut(s) 281, 2222, 3484
PsuI RGATCY 4 cut(s) 40, 556, 1114, 2549
PvuII CAGCTG 2 cut(s) 500, 2354
RseI CAYNNNNRTG 5 cut(s) 1412, 2121, 2210, 2529, 3803
SalI GTCGAC 1 cut(s) 1050
Sau96I GGNCC 2 cut(s) 1195, 3568
SchI GAGTC 4 cut(s) 834, 1047, 1135, 2228
ScrFI CCNGG 5 cut(s) 929, 1508, 2298, 2846, 3566
SduI GDGCHC 1 cut(s) 1500
SfcI CTRYAG 3 cut(s) 2187, 2715, 3185
Sfr274I CTCGAG 1 cut(s) 1499
SlaI CTCGAG 1 cut(s) 1499
SmiMI CAYNNNNRTG 5 cut(s) 1412, 2121, 2210, 2529, 3803
SmlI CTYRAG 4 cut(s) 463, 820, 1499, 2507
SmoI CTYRAG 4 cut(s) 463, 820, 1499, 2507
SpeI ACTAGT 1 cut(s) 53
SsiI CCGC 6 cut(s) 704, 808, 1348, 2150, 2991, 2994
SspI AATATT 2 cut(s) 2196, 2777
SspMI CTAG 4 cut(s) 54, 2165, 2330, 2619
StyD4I CCNGG 5 cut(s) 927, 1506, 2296, 2844, 3564
StyI CCWWGG 1 cut(s) 18
TaiI ACGT 4 cut(s) 75, 1063, 1696, 2140
TaqI TCGA 9 cut(s) 458, 512, 694, 1051, 1101, 1500, 1619, 2590, 3816
TatI WGTACW 8 cut(s) 1095, 1513, 1521, 1777, 2730, 2893, 3520, 3766
TauI GCSGC 2 cut(s) 811, 2996
TseFI GTSAC 3 cut(s) 49, 2291, 2962
TseI GCWGC 8 cut(s) 489, 2021, 2060, 2147, 2186, 2225, 2351, 3904
Tsp45I GTSAC 3 cut(s) 49, 2291, 2962
TspGWI ACGGA 1 cut(s) 2838
VspI ATTAAT 1 cut(s) 1086
XagI CCTNNNNNAGG 2 cut(s) 2365, 3575
XceI RCATGY 1 cut(s) 455
XhoI CTCGAG 1 cut(s) 1499
XmiI GTMKAC 2 cut(s) 1051, 2886
XmnI GAANNNNTTC 3 cut(s) 2196, 3470, 3864
XspI CTAG 4 cut(s) 54, 2165, 2330, 2619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.