RchiOBHm_Chr3g0486161

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000223
Physical Location & Seq
Forward (+)
33094679 .. 33099170
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45068

Sequence Viewer

Length: 3147 bp
ATGGAAACTATTGACAGTGCTCCATTTGCGGAAGTAGTTGATTTTAAAGAGTCCAAGCCATATGGAACAAAGCAATATACAGTCAAGGTTGATTGTTGGCGAAACAGGTTCAGTGAACGTGGCAATGATCCATACAAAACTTTGCCTGGGGATCTTTTTATTTTAGCAAATGCTAAACCTGAAACTGTTTCTGAATTACAACTGGCTGGCAGGTCATGGTCTTTTGTATCTGTAACTCATCTTGTGTCTAACATCTCACAAATTCAGAAAAAAGATGAGAATGGGGATGAGGAAACTTCTCTTTGTTTTAAAATCGAGTCTTCAAAAGAGTTTGAAGTCGATGATAGCACATCACTGTTTATTGTTTTCTTGTTGAATTTAATCCCCAGTCGAAGAATATACAAAGCATTGTACATGCAGGGAAATTTGACAATTATCAAAGAAGTTCTATGCTCTGATTCTGTGGCTCAGGAAAATCCTTCCTATGAGAAGAATAATGACATCATGGAGAAGGGGTTAGATGAGAATTTATTGTCAGGACTTAATGAGGCCCAAACTGGGGCAGTTTTGGCTTGTCTTGGAATGCGGCATTGTGGTGCAAAGTCTTCGGTGGAACTTATATGGGGTCCTCCTGGGACAGGAAAAACTAAAACAATCGCTACTCTGCTTTTTACTCTGTTAAAGAAGAATTGTAGGACTCTTATTTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGTCTTCTGAAAATGGTGACAGAAGCGGAGTCCAATGCAATGTTTTGTTCAGTTGGAAAAATCCTTTTACTTGGGAATAAGGAACGACTCAAACTCAAAGTTGGTTCAGATATTGAAGAGATATATTTGGGCCACCGTATTGAAAAAATCATCGAGTGCATAGGACCACAAACTGGTTGGAGGCATTGCTTTGCTTCCATGATAAATTTACTTGAAGATTGTGTTTGTGATTACAATATTTACTTGGGCAATGAGTTGACTGAACTCACTGAAGGGGAAGAACAAAATAGTGCTCGTGAAGTGAATGATGAACGCAGAAGTGGACCAGGAGTTAGCGAGGGGAAGTGCAAAACATTTCTTGAATATTTCAGAAATAGATTTCTCTCTACCGCATTGCCACTAAGATATTGCATTTCTATCTTCTGTACCCATATGACCAAAAGCTGCATTCCAGAACACATATTCAAGGATATGGTTTCTCTTATCCTCTTAGTTGATTCCTTTGGGATGCTGTTGTTTGAAGGTAATGTTATTTCAGAAGCATTGGAAGGCCGTTTTTCTTGTTCAGAGGTTGAGGGTGTATCTGAGTTATATGTAGCTGACTCAATTCCTCTTGTCATGAAGAGAAAGGAATGCCTTGTAGCTTTACGCACTCTACAGTATTCTCTTAGTAAACTTCAACTTCCAGATTTTAGGAACGGAACTGATCTAATGGATTTATGTTTCCTAGGTGCATCCTTAGTATTTTGCACTGCATCTAGTTCATTTAAGCTGCATAGACAGGAAATGGAGCCATTTACCTTTGTGGTTATTGATGAAGCTGCACAGTTGAAAGAGTGCGAGTCAACTATACCCCTGCAACTACCAGGAGTAAAACATGTAATTCTTGTTGGTGATGAACGCCAATTGCCAGCTATGGTGAAAAGCAATGTTTCTCAAGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCACAATGGGTCACCCAAAACACCTTCTAAATATGCAGTACAGAATGCATCCGTCAATAAGTTTCTTCCCAAATTCTAGTTTCTATAATAACCTGATCCTGGATGCTCCAAATGTCACAAAAAAAAGCCATGAGAAGCACTATCTTCCAGGGCCAATATATGGTCCTTATTCTTTTATAAATGTAAATGATGGAAGAGAGGAGAAGGATGAGGATGGACATAGTCGAAAGAATATGGTTGAGGTTGCTATTGTTTTGAAAGTACTGCGGAATCTTTACAAAGAATGGTTGAATTCAAAACAGAAACTCAATGTTGGTGTGGTATCTCCCTATGCTGCTCAAGTAGTGGCAATTCAGGAAAATCTTGGACAAAAGTATGACAATCTTGATGGCTTTAAAGTGAAGATAAATACAGTTGATGGGTTCCAGGGTGGGGAAGACGACATAATTATAATGTCCACTGTAAGATCCAACAGCCATCAATCACTTCAGTTTATTTCAAATATTCAAAGAGTCAATGTTGCTCTCACAAGGGCTAGGCACTGTCTCTGGATTTTGGGCAACGAAAAAACTCTATGCAGTAGTGATTCTGTTTGGAAGGACTTGGTTCTTGATGCCAAAAGTCGTGAATGCTTCTTTAATGCTGATCAAGACAAGGATTTGGCCAAGGCCATAGTAGACGTAAAGAAAGAGTTGGACCAATTGGATGATTTGCTCAACGCCGACAGCATACTTTTCAAAAATGCCAGATGGAAGGTGCTTTTTAGTGATAACTTTTTGAAGTCATTCAAGAAACTGACGTCAGCCTCTTTGAAGAAGTCAGTGCTAAACCTTCTACTGAAGCTTTCAACAGGCTGGAGACCTAGGAAGCGCAATGTAGAGACAGGTTGTAGTAGCTCTTCACTAATCTTGAAGAAATTTAAGGTTAAATCTCTATATATTGTTTGCACAACAGACATTGCAAAGGATATTGTTTGCACAACAGACACTGCGAAGGATATGAGATACATTCAAATATTAAAGGTCTGGGACATATTGCTCCTGGAGGATATCCCAAAGTTGGAATATCGCCTGGAGACTATTCTAAACAGATATACAGATGATTTTATTAGTCTTTGCAAGGAGAAATATCTTGAGGGTGATTTAGAAGTTCCACAGAGCTGGCCACTTTCTCTGGAATTTTCCCGGTTCAAAGATCCTACTGTCACTGAAACCAAGAGAGATTTTGTTGGTGACACTTCTGATGGCAGGAGTTATGTTGAGAATTCACAGGTCAGCGAGAGTTTGTTGCTAATGAAATTTTACTCTTTATCATCTGGTTTGGTGAACCACTTACTCTCTGACTCTGGGGTAGAGAATTGGATCTCCCGTTTGAAGTTACAGACCAAGAAAGGGAGATTATACTTCATCATAGAAGTTCTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1048

Amino Acids

118.55

Weight (kDa)

6.2

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 8 - 143 9.8e-51 Domain of unknown function (DUF6469)
AAA_11 PF13086 181 - 558 4.2e-35 AAA domain
AAA_19 PF13245 201 - 554 1.7e-10 AAA domain
AAA_12 PF13087 566 - 762 3.7e-57 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1898, 2171
AatII GACGTC 1 cut(s) 2522
Acc36I ACCTGC 1 cut(s) 201
AccB7I CCANNNNNTGG 2 cut(s) 917, 1880
AccI GTMKAC 1 cut(s) 2397
AciI CCGC 5 cut(s) 29, 586, 770, 1134, 1987
AclWI GGATC 6 cut(s) 122, 159, 1810, 2181, 2909, 3089
AcoI YGGCCR 2 cut(s) 2382, 2882
AcuI CTGAAG 4 cut(s) 753, 1035, 2192, 2579
AcyI GRCGYC 1 cut(s) 2519
AfaI GTAC 4 cut(s) 413, 1171, 1760, 1983
AfiI CCNNNNNNNGG 9 cut(s) 558, 559, 638, 917, 1819, 1880, 2152, 2779, 3111
AflIII ACRYGT 1 cut(s) 1621
AjnI CCWGG 9 cut(s) 145, 631, 1069, 1609, 1818, 1867, 2145, 2760, 2790
Alw21I GWGCWC 2 cut(s) 22, 1039
Alw26I GTCTC 4 cut(s) 2270, 2572, 2594, 2789
AlwI GGATC 6 cut(s) 122, 159, 1810, 2181, 2909, 3089
AlwNI CAGNNNCTG 1 cut(s) 2708
AoxI GGCC 7 cut(s) 549, 874, 1294, 1871, 2382, 2388, 2882
ApeKI GCWGC 4 cut(s) 1188, 1516, 1565, 2054
Asp700I GAANNNNTTC 1 cut(s) 2504
AspA2I CCTAGG 2 cut(s) 1471, 2582
AspLEI GCGC 1 cut(s) 2592
AspS9I GGNCC 8 cut(s) 550, 626, 874, 908, 1067, 1871, 1883, 2416
AsuC2I CCSGG 1 cut(s) 2905
AsuHPI GGTGA 7 cut(s) 772, 1649, 1675, 1724, 2870, 2963, 3055
AvaII GGWCC 5 cut(s) 626, 908, 1067, 1883, 2416
AvrII CCTAGG 2 cut(s) 1471, 2582
BaeGI GKGCMC 1 cut(s) 712
BalI TGGCCA 2 cut(s) 2384, 2884
BarI GAAGNNNNNNTAC 2 cut(s) 2602, 2634
BauI CACGAG 1 cut(s) 1038
BbsI GAAGAC 4 cut(s) 312, 597, 740, 2163
Bbv12I GWGCWC 2 cut(s) 22, 1039
BbvI GCAGC 4 cut(s) 1175, 1503, 1552, 2041
BccI CCATC 7 cut(s) 1904, 1928, 2102, 2132, 2205, 2463, 2957
BceAI ACGGC 1 cut(s) 1281
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 9 cut(s) 147, 633, 1071, 1611, 1820, 1869, 2147, 2762, 2792
BclI TGATCA 1 cut(s) 2365
BcnI CCSGG 1 cut(s) 2905
BcoDI GTCTC 4 cut(s) 2270, 2572, 2594, 2789
BfaI CTAG 5 cut(s) 1472, 1503, 1797, 2256, 2583
BfmI CTRYAG 1 cut(s) 1400
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 5 cut(s) 587, 1189, 1517, 1566, 2055
BlnI CCTAGG 2 cut(s) 1471, 2582
BlsI GCNGC 5 cut(s) 588, 1190, 1518, 1567, 2056
BmcAI AGTACT 1 cut(s) 1983
Bme18I GGWCC 5 cut(s) 626, 908, 1067, 1883, 2416
BmgT120I GGNCC 8 cut(s) 550, 626, 874, 908, 1067, 1871, 1883, 2416
BmiI GGNNCC 3 cut(s) 627, 1536, 2144
BmrI ACTGGG 2 cut(s) 381, 567
BmsI GCATC 6 cut(s) 1242, 1487, 1508, 1777, 1813, 2323
BmuI ACTGGG 2 cut(s) 381, 567
BpiI GAAGAC 4 cut(s) 312, 597, 740, 2163
BpmI CTGGAG 3 cut(s) 2596, 2783, 2813
Bpu10I CCTNAGC 1 cut(s) 468
BpuEI CTTGAG 3 cut(s) 1665, 2043, 2873
BpuMI CCSGG 1 cut(s) 2905
BsaHI GRCGYC 1 cut(s) 2519
BsaI GGTCTC 1 cut(s) 2572
BsaJI CCNNGG 7 cut(s) 146, 632, 1471, 1868, 2146, 2385, 2582
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 9 cut(s) 558, 559, 638, 917, 1819, 1880, 2152, 2779, 3111
Bse1I ACTGG 4 cut(s) 207, 387, 562, 922
Bse3DI GCAATG 8 cut(s) 130, 789, 928, 1000, 1136, 1678, 2599, 2676
BseBI CCWGG 9 cut(s) 147, 633, 1071, 1611, 1820, 1869, 2147, 2762, 2792
BseDI CCNNGG 7 cut(s) 146, 632, 1471, 1868, 2146, 2385, 2582
BseGI GGATG 8 cut(s) 292, 1257, 1478, 1768, 1828, 1933, 1939, 2431
BseLI CCNNNNNNNGG 9 cut(s) 558, 559, 638, 917, 1819, 1880, 2152, 2779, 3111
BseMI GCAATG 8 cut(s) 130, 789, 928, 1000, 1136, 1678, 2599, 2676
BseMII CTCAG 2 cut(s) 482, 1320
BseNI ACTGG 4 cut(s) 207, 387, 562, 922
BseRI GAGGAG 1 cut(s) 1934
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 4 cut(s) 1175, 1503, 1552, 2041
BsgI GTGCAG 1 cut(s) 1551
BshFI GGCC 7 cut(s) 551, 876, 1296, 1873, 2384, 2390, 2884
BsiHKAI GWGCWC 2 cut(s) 22, 1039
BsiSI CCGG 1 cut(s) 2905
BslFI GGGAC 2 cut(s) 649, 2762
BslI CCNNNNNNNGG 9 cut(s) 558, 559, 638, 917, 1819, 1880, 2152, 2779, 3111
BsmAI GTCTC 4 cut(s) 2270, 2572, 2594, 2789
BsmFI GGGAC 2 cut(s) 649, 2762
BsmI GAATGC 5 cut(s) 588, 1191, 1382, 1770, 2354
BsnI GGCC 7 cut(s) 551, 876, 1296, 1873, 2384, 2390, 2884
Bso31I GGTCTC 1 cut(s) 2572
Bsp1286I GDGCHC 3 cut(s) 22, 712, 1039
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
BspACI CCGC 5 cut(s) 29, 586, 770, 1134, 1987
BspANI GGCC 7 cut(s) 551, 876, 1296, 1873, 2384, 2390, 2884
BspCNI CTCAG 2 cut(s) 481, 1321
BspHI TCATGA 1 cut(s) 1362
BspLI GGNNCC 3 cut(s) 627, 1536, 2144
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 6 cut(s) 122, 159, 1810, 2181, 2909, 3089
BspQI GCTCTTC 1 cut(s) 2623
BspTNI GGTCTC 1 cut(s) 2572
BsrDI GCAATG 8 cut(s) 130, 789, 928, 1000, 1136, 1678, 2599, 2676
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 4 cut(s) 207, 387, 562, 922
BssECI CCNNGG 7 cut(s) 146, 632, 1471, 1868, 2146, 2385, 2582
BssMI GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
BssNI GRCGYC 1 cut(s) 2519
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 3 cut(s) 1471, 2385, 2582
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 9 cut(s) 147, 633, 1071, 1611, 1820, 1869, 2147, 2762, 2792
Bst6I CTCTTC 4 cut(s) 855, 1361, 1909, 2623
BstACI GRCGYC 1 cut(s) 2519
BstAUI TGTACA 1 cut(s) 411
BstC8I GCNNGC 3 cut(s) 208, 1656, 2882
BstDEI CTNAG 6 cut(s) 468, 1145, 1234, 1329, 1412, 1483
BstEII GGTNACC 1 cut(s) 1730
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 8 cut(s) 292, 1257, 1478, 1768, 1828, 1933, 1939, 2431
BstHHI GCGC 1 cut(s) 2592
BstKTI GATC 8 cut(s) 130, 154, 1453, 1818, 2189, 2368, 2917, 3084
BstMAI GTCTC 4 cut(s) 2270, 2572, 2594, 2789
BstMBI GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
BstMWI GCNNNNNNNGC 3 cut(s) 26, 569, 1694
BstNI CCWGG 9 cut(s) 147, 633, 1071, 1611, 1820, 1869, 2147, 2762, 2792
BstNSI RCATGY 2 cut(s) 418, 1625
BstPI GGTNACC 1 cut(s) 1730
BstSFI CTRYAG 1 cut(s) 1400
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 4 cut(s) 1175, 1503, 1552, 2041
BstV2I GAAGAC 4 cut(s) 312, 597, 740, 2163
BstX2I RGATCY 4 cut(s) 151, 2186, 2914, 3081
BstXI CCANNNNNNTGG 1 cut(s) 2880
BstYI RGATCY 4 cut(s) 151, 2186, 2914, 3081
BsuRI GGCC 7 cut(s) 551, 876, 1296, 1873, 2384, 2390, 2884
BtsCI GGATG 8 cut(s) 292, 1257, 1478, 1768, 1828, 1933, 1939, 2431
BtsI GCAGTG 2 cut(s) 1494, 2706
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 208, 1656, 2882
CaiI CAGNNNCTG 1 cut(s) 2708
CciI TCATGA 1 cut(s) 1362
CfoI GCGC 1 cut(s) 2592
Cfr13I GGNCC 8 cut(s) 550, 626, 874, 908, 1067, 1871, 1883, 2416
Csp6I GTAC 4 cut(s) 412, 1170, 1759, 1982
CviAII CATG 7 cut(s) 216, 415, 505, 943, 1363, 1622, 1850
CviQI GTAC 4 cut(s) 412, 1170, 1759, 1982
DdeI CTNAG 6 cut(s) 468, 1145, 1234, 1329, 1412, 1483
DpnI GATC 8 cut(s) 129, 153, 1452, 1817, 2188, 2367, 2916, 3083
DpnII GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
DraI TTTAAA 3 cut(s) 46, 310, 2116
EaeI YGGCCR 2 cut(s) 2382, 2882
Eam1104I CTCTTC 4 cut(s) 855, 1361, 1909, 2623
EarI CTCTTC 4 cut(s) 855, 1361, 1909, 2623
Eco130I CCWWGG 3 cut(s) 1471, 2385, 2582
Eco31I GGTCTC 1 cut(s) 2572
Eco32I GATATC 1 cut(s) 2770
Eco47I GGWCC 5 cut(s) 626, 908, 1067, 1883, 2416
Eco57I CTGAAG 4 cut(s) 753, 1035, 2192, 2579
Eco91I GGTNACC 1 cut(s) 1730
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 626
EcoO65I GGTNACC 1 cut(s) 1730
EcoRI GAATTC 2 cut(s) 2011, 2983
EcoRII CCWGG 9 cut(s) 145, 631, 1069, 1609, 1818, 1867, 2145, 2760, 2790
EcoRV GATATC 1 cut(s) 2770
EcoT14I CCWWGG 3 cut(s) 1471, 2385, 2582
EcoT22I ATGCAT 1 cut(s) 1770
ErhI CCWWGG 3 cut(s) 1471, 2385, 2582
FaeI CATG 7 cut(s) 219, 418, 508, 946, 1366, 1625, 1853
FaqI GGGAC 2 cut(s) 649, 2762
FatI CATG 7 cut(s) 215, 414, 504, 942, 1362, 1621, 1849
FauNDI CATATG 2 cut(s) 61, 1176
FbaI TGATCA 1 cut(s) 2365
FblI GTMKAC 1 cut(s) 2397
Fnu4HI GCNGC 5 cut(s) 587, 1189, 1517, 1566, 2055
FokI GGATG 8 cut(s) 299, 1264, 1465, 1755, 1835, 1940, 1946, 2438
Fsp4HI GCNGC 5 cut(s) 587, 1189, 1517, 1566, 2055
FspBI CTAG 5 cut(s) 1472, 1503, 1797, 2256, 2583
GlaI GCGC 1 cut(s) 2591
GluI GCNGC 5 cut(s) 587, 1189, 1517, 1566, 2055
GsuI CTGGAG 3 cut(s) 2596, 2783, 2813
HaeIII GGCC 7 cut(s) 551, 876, 1296, 1873, 2384, 2390, 2884
HapII CCGG 1 cut(s) 2905
HhaI GCGC 1 cut(s) 2592
Hin1I GRCGYC 1 cut(s) 2519
Hin1II CATG 7 cut(s) 219, 418, 508, 946, 1366, 1625, 1853
Hin6I GCGC 1 cut(s) 2590
HinP1I GCGC 1 cut(s) 2590
HincII GTYRAC 2 cut(s) 1002, 1590
HindII GTYRAC 2 cut(s) 1002, 1590
HindIII AAGCTT 2 cut(s) 1702, 2561
HpaII CCGG 1 cut(s) 2905
HphI GGTGA 7 cut(s) 772, 1649, 1675, 1724, 2870, 2963, 3055
Hpy166II GTNNAC 8 cut(s) 116, 1002, 1067, 1418, 1590, 2178, 2398, 3046
Hpy8I GTNNAC 8 cut(s) 116, 1002, 1067, 1418, 1590, 2178, 2398, 3046
HpyCH4IV ACGT 3 cut(s) 118, 2400, 2519
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 569, 1694
HpyF3I CTNAG 6 cut(s) 468, 1145, 1234, 1329, 1412, 1483
HpySE526I ACGT 3 cut(s) 118, 2400, 2519
Hsp92I GRCGYC 1 cut(s) 2519
Hsp92II CATG 7 cut(s) 219, 418, 508, 946, 1366, 1625, 1853
HspAI GCGC 1 cut(s) 2590
Ksp22I TGATCA 1 cut(s) 2365
Kzo9I GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
LguI GCTCTTC 1 cut(s) 2623
LmnI GCTCC 4 cut(s) 25, 1534, 1831, 2763
Lsp1109I GCAGC 4 cut(s) 1175, 1503, 1552, 2041
LweI GCATC 6 cut(s) 1242, 1487, 1508, 1777, 1813, 2323
MaeI CTAG 5 cut(s) 1472, 1503, 1797, 2256, 2583
MaeII ACGT 3 cut(s) 118, 2400, 2519
MaeIII GTNAC 7 cut(s) 232, 760, 1730, 1834, 2923, 2951, 3096
MalI GATC 8 cut(s) 129, 153, 1452, 1817, 2188, 2367, 2916, 3083
MboI GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
MfeI CAATTG 2 cut(s) 1649, 2420
MflI RGATCY 4 cut(s) 151, 2186, 2914, 3081
MhlI GDGCHC 3 cut(s) 22, 712, 1039
MlsI TGGCCA 2 cut(s) 2384, 2884
MluNI TGGCCA 2 cut(s) 2384, 2884
MlyI GAGTC 9 cut(s) 59, 326, 691, 782, 825, 1340, 1595, 2241, 3056
MmeI TCCRAC 5 cut(s) 778, 902, 2214, 2394, 2760
Mox20I TGGCCA 2 cut(s) 2384, 2884
Mph1103I ATGCAT 1 cut(s) 1770
MroXI GAANNNNTTC 1 cut(s) 2504
MscI TGGCCA 2 cut(s) 2384, 2884
MslI CAYNNNNRTG 1 cut(s) 594
Msp20I TGGCCA 2 cut(s) 2384, 2884
MspI CCGG 1 cut(s) 2905
MunI CAATTG 2 cut(s) 1649, 2420
Mva1269I GAATGC 5 cut(s) 588, 1191, 1382, 1770, 2354
MvaI CCWGG 9 cut(s) 147, 633, 1071, 1611, 1820, 1869, 2147, 2762, 2792
MwoI GCNNNNNNNGC 3 cut(s) 26, 569, 1694
NciI CCSGG 1 cut(s) 2905
NdeI CATATG 2 cut(s) 61, 1176
NdeII GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
NlaIII CATG 7 cut(s) 219, 418, 508, 946, 1366, 1625, 1853
NlaIV GGNNCC 3 cut(s) 627, 1536, 2144
NmuCI GTSAC 5 cut(s) 760, 1730, 1834, 2923, 2951
NsiI ATGCAT 1 cut(s) 1770
NspI RCATGY 2 cut(s) 418, 1625
PagI TCATGA 1 cut(s) 1362
PciI ACATGT 1 cut(s) 1621
PciSI GCTCTTC 1 cut(s) 2623
PctI GAATGC 5 cut(s) 588, 1191, 1382, 1770, 2354
PdmI GAANNNNTTC 1 cut(s) 2504
PfeI GAWTC 4 cut(s) 458, 1241, 1990, 2306
PflFI GACNNNGTC 1 cut(s) 1941
PflMI CCANNNNNTGG 2 cut(s) 917, 1880
PfoI TCCNGGA 2 cut(s) 1818, 2760
PkrI GCNGC 5 cut(s) 588, 1190, 1518, 1567, 2056
PleI GAGTC 9 cut(s) 58, 325, 691, 781, 825, 1340, 1594, 2240, 3056
PpsI GAGTC 9 cut(s) 58, 325, 691, 781, 825, 1340, 1594, 2240, 3056
PpuMI RGGWCCY 1 cut(s) 626
PscI ACATGT 1 cut(s) 1621
PsiI TTATAA 2 cut(s) 1898, 2171
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 9 cut(s) 145, 631, 1069, 1609, 1818, 1867, 2145, 2760, 2790
PspEI GGTNACC 1 cut(s) 1730
PspGI CCWGG 9 cut(s) 145, 631, 1069, 1609, 1818, 1867, 2145, 2760, 2790
PspN4I GGNNCC 3 cut(s) 627, 1536, 2144
PspPI GGNCC 8 cut(s) 550, 626, 874, 908, 1067, 1871, 1883, 2416
PspPPI RGGWCCY 1 cut(s) 626
PstNI CAGNNNCTG 1 cut(s) 2708
PsuI RGATCY 4 cut(s) 151, 2186, 2914, 3081
PsyI GACNNNGTC 1 cut(s) 1941
RsaI GTAC 4 cut(s) 413, 1171, 1760, 1983
RsaNI GTAC 4 cut(s) 412, 1170, 1759, 1982
RseI CAYNNNNRTG 1 cut(s) 594
SapI GCTCTTC 1 cut(s) 2623
SatI GCNGC 5 cut(s) 587, 1189, 1517, 1566, 2055
Sau3AI GATC 8 cut(s) 127, 151, 1450, 1815, 2186, 2365, 2914, 3081
Sau96I GGNCC 8 cut(s) 550, 626, 874, 908, 1067, 1871, 1883, 2416
ScaI AGTACT 1 cut(s) 1983
SchI GAGTC 9 cut(s) 59, 326, 691, 782, 825, 1340, 1595, 2241, 3056
SduI GDGCHC 3 cut(s) 22, 712, 1039
SfaNI GCATC 6 cut(s) 1242, 1487, 1508, 1777, 1813, 2323
SfcI CTRYAG 1 cut(s) 1400
SinI GGWCC 5 cut(s) 626, 908, 1067, 1883, 2416
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 3 cut(s) 1680, 2058, 2852
SmoI CTYRAG 3 cut(s) 1680, 2058, 2852
SsiI CCGC 5 cut(s) 29, 586, 770, 1134, 1987
SspI AATATT 4 cut(s) 982, 1109, 2224, 2736
SspMI CTAG 5 cut(s) 1472, 1503, 1797, 2256, 2583
StyI CCWWGG 3 cut(s) 1471, 2385, 2582
TaiI ACGT 3 cut(s) 121, 2403, 2522
TaqI TCGA 5 cut(s) 315, 339, 391, 897, 1945
TatI WGTACW 3 cut(s) 411, 1758, 1981
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 4 cut(s) 458, 1241, 1990, 2306
TseFI GTSAC 5 cut(s) 760, 1730, 1834, 2923, 2951
TseI GCWGC 4 cut(s) 1188, 1516, 1565, 2054
Tsp45I GTSAC 5 cut(s) 760, 1730, 1834, 2923, 2951
TspDTI ATGAA 7 cut(s) 1068, 1379, 1497, 1575, 1656, 3029, 3115
TspGWI ACGGA 2 cut(s) 1458, 1761
Tth111I GACNNNGTC 1 cut(s) 1941
Van91I CCANNNNNTGG 2 cut(s) 917, 1880
VpaK11BI GGWCC 5 cut(s) 626, 908, 1067, 1883, 2416
XagI CCTNNNNNAGG 1 cut(s) 636
XceI RCATGY 2 cut(s) 418, 1625
XcmI CCANNNNNNNNNTGG 1 cut(s) 918
XmaJI CCTAGG 2 cut(s) 1471, 2582
XmiI GTMKAC 1 cut(s) 2397
XmnI GAANNNNTTC 1 cut(s) 2504
XspI CTAG 5 cut(s) 1472, 1503, 1797, 2256, 2583
ZraI GACGTC 1 cut(s) 2520
ZrmI AGTACT 1 cut(s) 1983
Zsp2I ATGCAT 1 cut(s) 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.