Prupe.8G106200_v2.0.a1

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000223
Physical Location & Seq
Reverse (-)
13733545 .. 13741578
8034 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G106200.2

Sequence Viewer

Length: 5286 bp
ATGATGGAAGGCTCTGAGAGCTTGACGGACATTCTGTTTTCTTGGTCTCTCCAAGACATTCTTAATGAAAATCTTTACCAGCACAAGGTGGAAAAGATTCCCGAATCATTTCTGTCGGTTCAAGATCATTTCGGGTCTTATTCATATCCTTTGTTGGACGACACTCGGACTCAACTGCGTTCGAGTATGGAAACTATGGACAGAGCGCCATATAGTAAGGTAATTTCTGTTGAAGAGTTGAATCCATATGGAACAAAGCTATATGATATCAAGGTTGATTGCTGGAGAAACCTGTCCACCGATTGCAGCAAAGGACCATACAAAATTCTGCCTGGAGATGTTTTTGTTTTAGCAGATACTAGACCCGAAACTGTTTCTGATTTACAGAAGTTAGGGAGCTCTTGGGCCTTTCTAGTAGTCACTGAGGTCTCCAAAAATGAGAACGAGGAAGATAGAACTGCTCTTCATTTTAAAGTCAAAGCGTCGAAAGAGTTTGAAGTCAACCATAGCATTCATACATCACTCTTTATGGTTTTCTTATTAAACATAGCTCCTTACATACGAATATGGAAGGCGATGCACATGTCTGGAAGTGCAACATGGAAGGTGGTATTCAGTGATAACTTTTTGAAATCGTTCAAAGAACTGAAGTCATTCCAATTGAAGATGTCAGTACTGAGTCTTCTACTTAGGATTTCGGGTGGCTGGAGACCCAGGAGAAAAAATGTGGAGATAGTTTGTAGAAGCTCTTCCATGATTTTACGGAAGTTTAAGGTTGAAGGGCTATACATTCTCAGCACAACAGACATAGTAAAAAATTCCAGATACGTTCAAGTGTTAAAAATCTGGGACATATTGCCTGATCTACAGGGTCTTGAAAAATTGGTGGACCGATTAGACTCTATATTTAAAAGATATACAGATGGTTTTATCAATCTCTGCAGGGAGAGATGTCTTGAGGGTGATGTGGAAGTTCCAGCAAGCTGGACACCCTCTTTGGATGTTCCCCGATTCAAAGATGTTAGCATCACGGAAATCCCGAATGATTTTGTTGGTTACTCTTCTGGAAATACAAGTTATGTTGAGCGTTGCGAGGTCCGCGACAGTTTGTTGCTGATGAAATTCTATTCCTTGTCATCTTCTGGGGCAATGAATGTCTTGCTTTCCGACCATGAGGGCAGAGAATTGGATCTCCCGTTTGAAGTTACTGACCAAGAAATGGAGACCATCCTTTACAATAGAAGTACATTTATTCAAGGACGGTCAGGCACTGGGAAGACAACAGTATTGACCATGAAGTTATATCAGAAAGAACAGAAGCACCGTAGGGCAGCAGAAGAGGGATTCTACGGTGTTGGGAGCAATACATTCAGACATGTTAGTCCGAATAATGAGGCTGAGCAGATTTCTTCTTCTTCATCTACTAATGTGACTGCTTTGCGCCAGCTTTTTGTGACTGTCAGTCCTAAACTCTGTTTTTCTGTCAAGCAACATATTTCACGATTGAAAAGTTTCGCTTGTGGTGGAAGCCCTTCAGGTCAATGCAGTTTGATTGATATGGATGATTTTGATGATGAGGAAGCACAATTCAAGGGTATCCCTGATTCTTTTCTTAATATTCCGCCCCAATGCTATCCTCTTTGCTTAACATTTCGTAAATTCTTGATGATGCTTGATGGAAGTTTGGGTAATTCTTACTTTGAAAGATTCCTTGACATCACTGAACTTCCTCACAACAGACTGCAAAGTTCAAGATCAGTTTTGCTGCAGAACTTTCTAAGCACAAAGGAGGTCAATTATGAGAGATTTAGTTCATCATATTGGCCTCGTTTTAATATCCAGTTAACAAAGAAGCTTGATGCTTCAAGAGTCTTTACTGAGATTATTTCTCAAATAAAAGGTGGTCTGGGTGCTATGGAAGCATGTGATGGAAAACTCAGCCGGCAGGATTATGCGCAAATGTCAGAGGGAAAGGCTTCCGACCTAGACAAAGAGAAGAGAGATAAAATATATGATATATTTGAGATTTATGAAAAAATGAAGAGAAGGAATGGTGAATTCGATATTGCTGATTTTGTGAATGATATTCACCGCCGTTTTAAACGTGAAAAATATGAGGGTGATGAGATTGACTTTGTGTACATTGATGAGGTTCAGGATCTTGCCATGAGCCAAATTGCACTGTTCAAATATATCTGCAGTAATGTTGAAGAGGGCTTTGTTTTTTCTGGTGATACAGTGCAAACCATTGCAAGGGGGATTCATTTTAGATTCCAAGATATACGACATCTGTTCTACAACAAGTTTGTATTGGATTCAAGAAGAAAGAAGCATAAAGAACAAATGGACAATGAGAAAATCTCAGAAATTTTTCATTTGACAGAAAACTTCCGAAGCCATGATGGTATACTGAAGCTATTGCAGAGCATTGTTGAACTACTTTATCATTTTTTTCCCCACTCAATTGATAAGTTGAAACCAGAAACAATTCCAGTATATGGGGAAGCTCCAACTTTAATTTACTCTGGAGAAAATGAAAATGTATTTGAAACAATTTTTGGAAATACTGAATTTGTTACTAGGAACACAATTGGTTTTGGGGCAGAGCAGGTTATTTTGGTACGTGACGCTAGTGCACGGAAGGAAATCTCCAACTCCATACGGAAGCAAGCACTTGTTCTTACTATAATGGACTGCAAGGGCCTAGAGTTTCAGGATGTACTCTTGTACAATTTTTTTGGATCATCACCCCTGAAAAATCAATGGAGAGTGATATATGATTTCATGAAGGAACAAGACAGAGGTTTTCCAAGCTTTGATGATGCAAAACACAGCACGTTATGCTACGAATTAAAGCAGTTATATGTTGCAGTCTCTCGTACGAGACAGAGGTTGTGGATTTATGAGAATGTTGAAGAGCTCTCCAATCCCATGTTTGACTACTGGAAAAAGAAATGTCTTGTACAAGTTAGACAACTTGATGATTCATTTGCACTTTCTATGCAAGTTGCAAGCAGTCCACAAGAGTGGAAAGCACGTGGAATTAAGCTATATCAAGATCATAACTACAAAATGGCCAGAATGTGCTTTGAAAAAGCTGGTGATACATTCTGGAAAAGGCGTTCTGAAGCTGCTGAACTTAAAGCTAAGGCTCACCATATGCGCACTTCAAATCCTGAAATGGCTAATACCATGCTTCGGAGGGCTGCTTTAATATTTGAAGCTATCGGCCTGTCTGTTTCTGCTGCCAGATGCTTTTACAATTTGGGAGAGTATAAACGAGCCGGCTATATTTATTTGGATAAATGCAATGAACCTGAACTGGAAAGAGCTGGGGAATGCTTTTCTCTAGCTGGATGCTATGCGCTTGCAGCAGATGCGTATGCAAGGGGGAATTATTTCTCTGAGTGTATTTCTATGTGTTCCAAAGGAAAACTATTTGACAGAGGTCTGGAATACATCAAAGATTGGAAACAACATGCAACGGCAGAGTATGGTAAACGAGGAAATGGGACTACTAAAACTGAATTGGATTTTCTAGAGATTTGTGCATTTCATTATTCTGTTGTGAAAGATGAGAGATCCAGGATGGAAACTGTCAGAGACGAAATGTTTTCAGCTCAGAAAACTTTGAATGGTCTTCTTTCTTCAAGCACCTCTAAGTTTTTGTGGGAAGACAAATTGATGGATAATCGTAAACAGCAGTCAGAAGGCAAAAAATATAAGACACGGGTTTCTGCTGATTCACTGCAGTACTTTTGGAATTCTTGGAAGGATAAAAGTATTTACTTGATTGAATATCTAGGGAACTTCGAATCGATAGACGCCAATGAATACAGAAATTATGAAGACTTCTTTCTAAATTACTTAGGAGTATGGAGGCTGTTTCATGAAGATCTGAATCCGATTTATCTTTCAGTCATCTCTGATGTGGATTGGATTAGAGGTGTGGAGAAAAGATTTTTTCGAAGTAACGGGGAGTTAGTCTCAATTGATGTTCACAAGTATGTTTCAGCTGCTCAGAATTATTGGAGTTCTGAAATGCTATATCTTGGCATTAAGGTTCTGGGCAAACTTGAAGCTCTTTACAGGTTCCTAAGCACTCAATCTCATTCAAAATTCTTCAGAAGCAAGTCTCTTATCCAGATATATGAGGTTGTGACATATCTTCTTGACTCTAAATTTCTGAAGCGGAGCCTGATAGATCGTGACACACTGCAGAAATTTGTTAAGTTGTCGGGTGATAATTTTGTTTCCTACATATTTCCTTTAGATTGGAGGAAATCATCAAGAGAGAATATGATATCTCTTCGACGGTCTGAAGCTTGTAAGAATTTACTGAAACAAGTGATAGTTGAATACATGAGCTCATCCAAAGACCTGTCTTCAGGGAAAATTGGATACCTTGCTTCAATAATTCTTGCGTCTGGTAAGCTGAATGATGAACTGTGTGCGACGCTTGTGAAAAATATTGAGTACAATCCACTATGGAAGGCGTTCATTGAAAATCTATGTGGCAACATCCAAGAACCAAGAGTGGAGTCTCTTTTGTTGACGTTTGGTGATGCGTTGTTCAAAACTTATAACGAAACTAGGAGTGCACATTACTACATATCACCTAGTTGCTTCTTGTATCTTGTTGATCGCCTTCTGATTTGGGTATCTCTGTGTAAAGGTTATGTGATCACTGAAAGATCTTGCTTTATTGAATGGCTGATATACCATGAGAAAGATACCGGATTTAATTCCTGTGAAGTTTTTGATGTGCGAATGTCTTTTGAAGTCATCCTTCAGTTTTTGACTGATGTTATTCGGGAGTGTCTCTTTGATGAGGCGACCATGATTGAGTGGATTGGAAATTTTACCACATATTCGAAGAAGTATTATTTGCTACTAATGCAAAGATTGGTTATGACATTGTGTTTGCTTTATTTGAATTTTGGGATAGGTTTTGATATACTCTTAGATCTGAAGAATTGGGAATATGTCACTGAGAATCTACTACCAAGGGAGATCTCTGCTGCCCTTAGAAGAATTATTTCTGTACAGAAGTCTCTTGGTATAAATGTCAATGTGCTTCCACTCAAAGAAAATGTCAATGTGCTTCCACTCAAAGAAAATGTCAATGTGCTAGCTAAAGCATTTAAGAAGACCGGCAGTGCTTTGGTAATTGCAACTTCTAGGATTGATTGTTCAATGTTCGTCTGTTCAGATGCCCTTTTTGTCGACATGAAGGGCAACCTGGGCATGGTTGATATATTGAGACATTTGTTTCCAGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1762

Amino Acids

204.01

Weight (kDa)

6.72

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4584
AasI GACNNNNNNGTC 1 cut(s) 1380
Acc16I TGCGCA 2 cut(s) 1956, 3164
Acc36I ACCTGC 1 cut(s) 2599
AccB7I CCANNNNNTGG 2 cut(s) 1219, 2498
AccI GTMKAC 2 cut(s) 2407, 5226
AccII CGCG 1 cut(s) 1101
AciI CCGC 4 cut(s) 1099, 1622, 2092, 4192
AclWI GGATC 4 cut(s) 1197, 2166, 2749, 3576
AcoI YGGCCR 1 cut(s) 3075
AcvI CACGTG 1 cut(s) 3038
AcyI GRCGYC 1 cut(s) 3825
AdeI CACNNNGTG 1 cut(s) 88
AfiI CCNNNNNNNGG 6 cut(s) 85, 1219, 2253, 2498, 3198, 5248
AflIII ACRYGT 2 cut(s) 582, 1375
AhdI GACNNNNNGTC 1 cut(s) 1461
AjnI CCWGG 4 cut(s) 331, 713, 3584, 5241
AjuI GAANNNNNNNTTGG 2 cut(s) 2541, 2573
AleI CACNNNNGTG 1 cut(s) 3025
AloI GAACNNNNNNTCC 4 cut(s) 3106, 3138, 5176, 5208
Alw21I GWGCWC 5 cut(s) 401, 2638, 2922, 4370, 4603
Alw44I GTGCAC 2 cut(s) 2634, 4599
AlwI GGATC 4 cut(s) 1197, 2166, 2749, 3576
AlwNI CAGNNNCTG 1 cut(s) 1271
AoxI GGCC 5 cut(s) 405, 1823, 2701, 3075, 3229
ApaLI GTGCAC 2 cut(s) 2634, 4599
ApeKI GCWGC 9 cut(s) 306, 1331, 1765, 3131, 3206, 3245, 3371, 4016, 5021
ArsI GACNNNNNNTTYG 8 cut(s) 979, 1011, 4906, 4938, 5079, 5106, 5111, 5138
AspLEI GCGC 5 cut(s) 208, 1443, 1957, 3165, 3367
AspS9I GGNCC 5 cut(s) 314, 405, 889, 1096, 2701
AsuII TTCGAA 3 cut(s) 3813, 3967, 4874
AsuNHI GCTAGC 1 cut(s) 5131
AvaII GGWCC 3 cut(s) 314, 889, 1096
BaeGI GKGCMC 2 cut(s) 2638, 4603
BalI TGGCCA 1 cut(s) 3077
BanII GRGCYC 3 cut(s) 401, 2922, 4370
BarI GAAGNNNNNNTAC 4 cut(s) 666, 698, 733, 765
BbrPI CACGTG 1 cut(s) 3038
BbsI GAAGAC 7 cut(s) 674, 1283, 3632, 3681, 3855, 4377, 5156
Bbv12I GWGCWC 5 cut(s) 401, 2638, 2922, 4370, 4603
BbvI GCAGC 9 cut(s) 318, 1343, 1752, 3118, 3193, 3232, 3383, 4003, 5008
BccI CCATC 7 cut(s) 917, 1235, 1670, 1922, 2396, 3583, 3679
BceAI ACGGC 2 cut(s) 2079, 3501
BciT130I CCWGG 4 cut(s) 333, 715, 3586, 5243
BciVI GTATCC 2 cut(s) 1607, 4394
BclI TGATCA 1 cut(s) 4683
BfmI CTRYAG 6 cut(s) 866, 940, 1766, 2197, 3749, 4217
BfoI RGCGCY 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 2599
BfuI GTATCC 2 cut(s) 1607, 4394
BglII AGATCT 4 cut(s) 3895, 4694, 4966, 5013
BisI GCNGC 9 cut(s) 307, 1332, 1766, 3132, 3207, 3246, 3372, 4017, 5022
BlpI GCTNAGC 1 cut(s) 1398
BlsI GCNGC 9 cut(s) 308, 1333, 1767, 3133, 3208, 3247, 3373, 4018, 5023
BmcAI AGTACT 2 cut(s) 675, 3755
Bme1390I CCNGG 4 cut(s) 333, 715, 3586, 5243
Bme18I GGWCC 3 cut(s) 314, 889, 1096
BmeRI GACNNNNNGTC 1 cut(s) 1461
BmgT120I GGNCC 5 cut(s) 314, 405, 889, 1096, 2701
BmiI GGNNCC 2 cut(s) 4094, 4196
BmrFI CCNGG 4 cut(s) 333, 715, 3586, 5243
BmrI ACTGGG 1 cut(s) 1281
BmtI GCTAGC 1 cut(s) 5135
BmuI ACTGGG 1 cut(s) 1281
BoxI GACNNNNGTC 1 cut(s) 3447
BpiI GAAGAC 7 cut(s) 674, 1283, 3632, 3681, 3855, 4377, 5156
BplI GAGNNNNNCTC 4 cut(s) 2345, 2377, 3971, 4003
BpmI CTGGAG 4 cut(s) 304, 354, 727, 2547
Bpu10I CCTNAGC 2 cut(s) 3147, 4097
Bpu1102I GCTNAGC 1 cut(s) 1398
Bpu14I TTCGAA 3 cut(s) 3813, 3967, 4874
BpuEI CTTGAG 1 cut(s) 977
Bsa29I ATCGAT 1 cut(s) 3818
BsaAI YACGTR 2 cut(s) 2624, 3038
BsaBI GATNNNNATC 1 cut(s) 3900
BsaHI GRCGYC 1 cut(s) 3825
BsaI GGTCTC 4 cut(s) 51, 433, 703, 1217
BsaJI CCNNGG 3 cut(s) 713, 5006, 5242
BsaWI WCCGGW 1 cut(s) 4736
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 1219, 2253, 2498, 3198, 5248
Bse118I RCCGGY 3 cut(s) 1941, 3284, 5153
Bse1I ACTGG 5 cut(s) 1276, 1840, 2492, 2948, 3327
Bse3DI GCAATG 3 cut(s) 1155, 2247, 3316
Bse8I GATNNNNATC 1 cut(s) 3900
BseBI CCWGG 4 cut(s) 333, 715, 3586, 5243
BseCI ATCGAT 1 cut(s) 3818
BseDI CCNNGG 3 cut(s) 713, 5006, 5242
BseGI GGATG 9 cut(s) 1006, 1227, 1567, 2722, 3362, 3594, 4370, 4521, 4785
BseJI GATNNNNATC 1 cut(s) 3900
BseLI CCNNNNNNNGG 6 cut(s) 85, 1219, 2253, 2498, 3198, 5248
BseMI GCAATG 3 cut(s) 1155, 2247, 3316
BseNI ACTGG 5 cut(s) 1276, 1840, 2492, 2948, 3327
BseSI GKGCMC 2 cut(s) 2638, 4603
BseXI GCAGC 9 cut(s) 318, 1343, 1752, 3118, 3193, 3232, 3383, 4003, 5008
BseYI CCCAGC 1 cut(s) 3332
Bsh1236I CGCG 1 cut(s) 1101
BshFI GGCC 5 cut(s) 407, 1825, 2703, 3077, 3231
BshVI ATCGAT 1 cut(s) 3818
BsiHKAI GWGCWC 5 cut(s) 401, 2638, 2922, 4370, 4603
BsiSI CCGG 4 cut(s) 1942, 3285, 4737, 5154
BsiWI CGTACG 1 cut(s) 2879
BslFI GGGAC 2 cut(s) 863, 3526
BslI CCNNNNNNNGG 6 cut(s) 85, 1219, 2253, 2498, 3198, 5248
BsmBI CGTCTC 1 cut(s) 3597
BsmFI GGGAC 2 cut(s) 863, 3526
BsmI GAATGC 2 cut(s) 510, 3344
BsnI GGCC 5 cut(s) 407, 1825, 2703, 3077, 3231
Bso31I GGTCTC 4 cut(s) 51, 433, 703, 1217
Bsp119I TTCGAA 3 cut(s) 3813, 3967, 4874
Bsp1286I GDGCHC 5 cut(s) 401, 2638, 2922, 4370, 4603
Bsp1407I TGTACA 4 cut(s) 2139, 2727, 2962, 5044
Bsp1720I GCTNAGC 1 cut(s) 1398
BspACI CCGC 4 cut(s) 1099, 1622, 2092, 4192
BspANI GGCC 5 cut(s) 407, 1825, 2703, 3077, 3231
BspDI ATCGAT 1 cut(s) 3818
BspFNI CGCG 1 cut(s) 1101
BspHI TCATGA 2 cut(s) 2784, 3889
BspLI GGNNCC 2 cut(s) 4094, 4196
BspMAI CTGCAG 5 cut(s) 944, 1770, 2201, 3753, 4221
BspMI ACCTGC 1 cut(s) 2599
BspOI GCTAGC 1 cut(s) 5135
BspPI GGATC 4 cut(s) 1197, 2166, 2749, 3576
BspQI GCTCTTC 3 cut(s) 468, 754, 2910
BspT104I TTCGAA 3 cut(s) 3813, 3967, 4874
BspTNI GGTCTC 4 cut(s) 51, 433, 703, 1217
BsrDI GCAATG 3 cut(s) 1155, 2247, 3316
BsrFI RCCGGY 3 cut(s) 1941, 3284, 5153
BsrGI TGTACA 4 cut(s) 2139, 2727, 2962, 5044
BsrI ACTGG 5 cut(s) 1276, 1840, 2492, 2948, 3327
BssAI RCCGGY 3 cut(s) 1941, 3284, 5153
BssECI CCNNGG 3 cut(s) 713, 5006, 5242
BssNAI GTATAC 1 cut(s) 2408
BssNI GRCGYC 1 cut(s) 3825
BssT1I CCWWGG 1 cut(s) 5006
Bst1107I GTATAC 1 cut(s) 2408
Bst2UI CCWGG 4 cut(s) 333, 715, 3586, 5243
BstACI GRCGYC 1 cut(s) 3825
BstAPI GCANNNNNTGC 2 cut(s) 2841, 3377
BstAUI TGTACA 4 cut(s) 2139, 2727, 2962, 5044
BstBAI YACGTR 2 cut(s) 2624, 3038
BstBI TTCGAA 3 cut(s) 3813, 3967, 4874
BstC8I GCNNGC 8 cut(s) 982, 1445, 1943, 2670, 3013, 3286, 3369, 5133
BstF5I GGATG 9 cut(s) 1006, 1227, 1567, 2722, 3362, 3594, 4370, 4521, 4785
BstFNI CGCG 1 cut(s) 1101
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 5 cut(s) 208, 1443, 1957, 3165, 3367
BstMWI GCNNNNNNNGC 8 cut(s) 18, 1098, 1919, 2841, 3371, 3377, 4897, 5244
BstNI CCWGG 4 cut(s) 333, 715, 3586, 5243
BstNSI RCATGY 4 cut(s) 586, 1379, 1926, 3482
BstPAI GACNNNNGTC 1 cut(s) 3447
BstSCI CCNGG 4 cut(s) 331, 713, 3584, 5241
BstSFI CTRYAG 6 cut(s) 866, 940, 1766, 2197, 3749, 4217
BstSLI GKGCMC 2 cut(s) 2638, 4603
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 9 cut(s) 318, 1343, 1752, 3118, 3193, 3232, 3383, 4003, 5008
BstV2I GAAGAC 7 cut(s) 674, 1283, 3632, 3681, 3855, 4377, 5156
BstX2I RGATCY 7 cut(s) 1189, 2158, 3581, 3895, 4694, 4966, 5013
BstXI CCANNNNNNTGG 2 cut(s) 984, 3027
BstYI RGATCY 7 cut(s) 1189, 2158, 3581, 3895, 4694, 4966, 5013
BstZ17I GTATAC 1 cut(s) 2408
Bsu15I ATCGAT 1 cut(s) 3818
BsuI GTATCC 2 cut(s) 1607, 4394
BsuRI GGCC 5 cut(s) 407, 1825, 2703, 3077, 3231
BsuTUI ATCGAT 1 cut(s) 3818
BtgZI GCGATG 1 cut(s) 590
BtsCI GGATG 9 cut(s) 1006, 1227, 1567, 2722, 3362, 3594, 4370, 4521, 4785
BtsI GCAGTG 3 cut(s) 3746, 4214, 5164
BveI ACCTGC 1 cut(s) 2599
Cac8I GCNNGC 8 cut(s) 982, 1445, 1943, 2670, 3013, 3286, 3369, 5133
CaiI CAGNNNCTG 1 cut(s) 1271
CciI TCATGA 2 cut(s) 2784, 3889
CfoI GCGC 5 cut(s) 208, 1443, 1957, 3165, 3367
Cfr10I RCCGGY 3 cut(s) 1941, 3284, 5153
Cfr13I GGNCC 5 cut(s) 314, 405, 889, 1096, 2701
ClaI ATCGAT 1 cut(s) 3818
CseI GACGC 5 cut(s) 471, 2636, 3833, 4413, 4465
DraI TTTAAA 3 cut(s) 472, 910, 2101
DraIII CACNNNGTG 1 cut(s) 88
DrdI GACNNNNNNGTC 1 cut(s) 1380
DriI GACNNNNNGTC 1 cut(s) 1461
DseDI GACNNNNNNGTC 1 cut(s) 1380
EaeI YGGCCR 1 cut(s) 3075
Eam1105I GACNNNNNGTC 1 cut(s) 1461
EciI GGCGGA 1 cut(s) 1611
Ecl136II GAGCTC 3 cut(s) 399, 2920, 4368
Eco130I CCWWGG 1 cut(s) 5006
Eco24I GRGCYC 3 cut(s) 401, 2922, 4370
Eco31I GGTCTC 4 cut(s) 51, 433, 703, 1217
Eco32I GATATC 2 cut(s) 268, 4305
Eco47I GGWCC 3 cut(s) 314, 889, 1096
Eco53kI GAGCTC 3 cut(s) 399, 2920, 4368
Eco72I CACGTG 1 cut(s) 3038
EcoICRI GAGCTC 3 cut(s) 399, 2920, 4368
EcoO109I RGGNCCY 1 cut(s) 2701
EcoRI GAATTC 2 cut(s) 2057, 3763
EcoRII CCWGG 4 cut(s) 331, 713, 3584, 5241
EcoRV GATATC 2 cut(s) 268, 4305
EcoT14I CCWWGG 1 cut(s) 5006
EcoT38I GRGCYC 3 cut(s) 401, 2922, 4370
ErhI CCWWGG 1 cut(s) 5006
Esp3I CGTCTC 1 cut(s) 3597
FalI AAGNNNNNCTT 6 cut(s) 45, 77, 1501, 1533, 4773, 4805
FaqI GGGAC 2 cut(s) 863, 3526
FauNDI CATATG 2 cut(s) 247, 3159
FbaI TGATCA 1 cut(s) 4683
FblI GTMKAC 2 cut(s) 2407, 5226
Fnu4HI GCNGC 9 cut(s) 307, 1332, 1766, 3132, 3207, 3246, 3372, 4017, 5022
FokI GGATG 9 cut(s) 1013, 1214, 1574, 2729, 3369, 3601, 4357, 4508, 4772
FriOI GRGCYC 3 cut(s) 401, 2922, 4370
Fsp4HI GCNGC 9 cut(s) 307, 1332, 1766, 3132, 3207, 3246, 3372, 4017, 5022
FspAI RTGCGCAY 1 cut(s) 3164
FspI TGCGCA 2 cut(s) 1956, 3164
GlaI GCGC 5 cut(s) 207, 1442, 1956, 3164, 3366
GluI GCNGC 9 cut(s) 307, 1332, 1766, 3132, 3207, 3246, 3372, 4017, 5022
GsaI CCCAGC 1 cut(s) 3336
GsuI CTGGAG 4 cut(s) 304, 354, 727, 2547
HaeII RGCGCY 1 cut(s) 209
HaeIII GGCC 5 cut(s) 407, 1825, 2703, 3077, 3231
HapII CCGG 4 cut(s) 1942, 3285, 4737, 5154
HgaI GACGC 5 cut(s) 471, 2636, 3833, 4413, 4465
HhaI GCGC 5 cut(s) 208, 1443, 1957, 3165, 3367
Hin1I GRCGYC 1 cut(s) 3825
Hin6I GCGC 5 cut(s) 206, 1441, 1955, 3163, 3365
HinP1I GCGC 5 cut(s) 206, 1441, 1955, 3163, 3365
HincII GTYRAC 4 cut(s) 502, 1845, 4554, 5227
HindII GTYRAC 4 cut(s) 502, 1845, 4554, 5227
HindIII AAGCTT 3 cut(s) 1853, 2812, 4323
HpaI GTTAAC 1 cut(s) 1845
HpaII CCGG 4 cut(s) 1942, 3285, 4737, 5154
Hpy99I CGWCG 3 cut(s) 487, 4317, 4459
HpyCH4IV ACGT 6 cut(s) 828, 2104, 2623, 2837, 3037, 4556
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 1098, 1919, 2841, 3371, 3377, 4897, 5244
HpySE526I ACGT 6 cut(s) 828, 2104, 2623, 2837, 3037, 4556
Hsp92I GRCGYC 1 cut(s) 3825
HspAI GCGC 5 cut(s) 206, 1441, 1955, 3163, 3365
KroI GCCGGC 2 cut(s) 1941, 3284
KroNI GCCGGC 2 cut(s) 1943, 3286
Ksp22I TGATCA 1 cut(s) 4683
KspAI GTTAAC 1 cut(s) 1845
LguI GCTCTTC 3 cut(s) 468, 754, 2910
LmnI GCTCC 5 cut(s) 396, 556, 1359, 2512, 4194
Lsp1109I GCAGC 9 cut(s) 318, 1343, 1752, 3118, 3193, 3232, 3383, 4003, 5008
MaeII ACGT 6 cut(s) 828, 2104, 2623, 2837, 3037, 4556
MfeI CAATTG 4 cut(s) 659, 2463, 2589, 3990
MflI RGATCY 7 cut(s) 1189, 2158, 3581, 3895, 4694, 4966, 5013
MhlI GDGCHC 5 cut(s) 401, 2638, 2922, 4370, 4603
MlsI TGGCCA 1 cut(s) 3077
MluNI TGGCCA 1 cut(s) 3077
MlyI GAGTC 6 cut(s) 163, 688, 893, 1878, 4169, 4550
MmeI TCCRAC 5 cut(s) 135, 1191, 2004, 2534, 2676
Mox20I TGGCCA 1 cut(s) 3077
MroNI GCCGGC 2 cut(s) 1941, 3284
MscI TGGCCA 1 cut(s) 3077
MslI CAYNNNNRTG 2 cut(s) 3025, 4005
Msp20I TGGCCA 1 cut(s) 3077
MspA1I CMGCKG 1 cut(s) 4016
MspI CCGG 4 cut(s) 1942, 3285, 4737, 5154
MspR9I CCNGG 4 cut(s) 333, 715, 3586, 5243
MunI CAATTG 4 cut(s) 659, 2463, 2589, 3990
Mva1269I GAATGC 2 cut(s) 510, 3344
MvaI CCWGG 4 cut(s) 333, 715, 3586, 5243
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 8 cut(s) 18, 1098, 1919, 2841, 3371, 3377, 4897, 5244
NaeI GCCGGC 2 cut(s) 1943, 3286
NdeI CATATG 2 cut(s) 247, 3159
NgoMIV GCCGGC 2 cut(s) 1941, 3284
NheI GCTAGC 1 cut(s) 5131
NlaIV GGNNCC 2 cut(s) 4094, 4196
NmuCI GTSAC 7 cut(s) 418, 1429, 1453, 2624, 4159, 4208, 4987
NsbI TGCGCA 2 cut(s) 1956, 3164
NspI RCATGY 4 cut(s) 586, 1379, 1926, 3482
NspV TTCGAA 3 cut(s) 3813, 3967, 4874
OliI CACNNNNGTG 1 cut(s) 3025
PagI TCATGA 2 cut(s) 2784, 3889
PciI ACATGT 2 cut(s) 582, 1375
PciSI GCTCTTC 3 cut(s) 468, 754, 2910
PcsI WCGNNNNNNNCGW 1 cut(s) 1037
PctI GAATGC 2 cut(s) 510, 3344
PdiI GCCGGC 2 cut(s) 1943, 3286
Pfl23II CGTACG 1 cut(s) 2879
PflMI CCANNNNNTGG 2 cut(s) 1219, 2498
PfoI TCCNGGA 1 cut(s) 3584
PkrI GCNGC 9 cut(s) 308, 1333, 1767, 3133, 3208, 3247, 3373, 4018, 5023
PleI GAGTC 6 cut(s) 163, 687, 893, 1877, 4169, 4549
PmaCI CACGTG 1 cut(s) 3038
PmlI CACGTG 1 cut(s) 3038
PpsI GAGTC 6 cut(s) 163, 687, 893, 1877, 4169, 4549
Ppu21I YACGTR 2 cut(s) 2624, 3038
PscI ACATGT 2 cut(s) 582, 1375
PshAI GACNNNNGTC 1 cut(s) 3447
PsiI TTATAA 1 cut(s) 4584
Psp124BI GAGCTC 3 cut(s) 401, 2922, 4370
Psp6I CCWGG 4 cut(s) 331, 713, 3584, 5241
PspCI CACGTG 1 cut(s) 3038
PspFI CCCAGC 1 cut(s) 3332
PspGI CCWGG 4 cut(s) 331, 713, 3584, 5241
PspLI CGTACG 1 cut(s) 2879
PspN4I GGNNCC 2 cut(s) 4094, 4196
PspPI GGNCC 5 cut(s) 314, 405, 889, 1096, 2701
PstI CTGCAG 5 cut(s) 944, 1770, 2201, 3753, 4221
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 7 cut(s) 1189, 2158, 3581, 3895, 4694, 4966, 5013
PvuII CAGCTG 1 cut(s) 4016
RseI CAYNNNNRTG 2 cut(s) 3025, 4005
SacI GAGCTC 3 cut(s) 401, 2922, 4370
SalI GTCGAC 1 cut(s) 5225
SapI GCTCTTC 3 cut(s) 468, 754, 2910
SatI GCNGC 9 cut(s) 307, 1332, 1766, 3132, 3207, 3246, 3372, 4017, 5022
Sau96I GGNCC 5 cut(s) 314, 405, 889, 1096, 2701
ScaI AGTACT 2 cut(s) 675, 3755
SchI GAGTC 6 cut(s) 163, 688, 893, 1878, 4169, 4550
ScrFI CCNGG 4 cut(s) 333, 715, 3586, 5243
SduI GDGCHC 5 cut(s) 401, 2638, 2922, 4370, 4603
SfcI CTRYAG 6 cut(s) 866, 940, 1766, 2197, 3749, 4217
SfuI TTCGAA 3 cut(s) 3813, 3967, 4874
SinI GGWCC 3 cut(s) 314, 889, 1096
SmiMI CAYNNNNRTG 2 cut(s) 3025, 4005
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SsiI CCGC 4 cut(s) 1099, 1622, 2092, 4192
SspI AATATT 3 cut(s) 1618, 3216, 4471
SstI GAGCTC 3 cut(s) 401, 2922, 4370
StyD4I CCNGG 4 cut(s) 331, 713, 3584, 5241
StyI CCWWGG 1 cut(s) 5006
TaiI ACGT 6 cut(s) 831, 2107, 2626, 2840, 3040, 4559
TaqI TCGA 9 cut(s) 182, 485, 2061, 3813, 3818, 3967, 4312, 4874, 5226
TaqII GACCGA 1 cut(s) 906
TatI WGTACW 9 cut(s) 673, 1244, 2139, 2719, 2727, 2962, 3753, 4476, 5044
TseFI GTSAC 7 cut(s) 418, 1429, 1453, 2624, 4159, 4208, 4987
TseI GCWGC 9 cut(s) 306, 1331, 1765, 3131, 3206, 3245, 3371, 4016, 5021
Tsp45I GTSAC 7 cut(s) 418, 1429, 1453, 2624, 4159, 4208, 4987
TspGWI ACGGA 5 cut(s) 41, 778, 1046, 2653, 2677
Van91I CCANNNNNTGG 2 cut(s) 1219, 2498
VneI GTGCAC 2 cut(s) 2634, 4599
VpaK11BI GGWCC 3 cut(s) 314, 889, 1096
XbaI TCTAGA 1 cut(s) 3538
XceI RCATGY 4 cut(s) 586, 1379, 1926, 3482
XmiI GTMKAC 2 cut(s) 2407, 5226
ZrmI AGTACT 2 cut(s) 675, 3755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.