FvH4_4g36280

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000223
Physical Location & Seq
Forward (+)
33280550 .. 33292786
12237 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36280.t2

Sequence Viewer

Length: 8106 bp
ATGATGGAGGTTTCAGGCAAGAAGAAGGTGAAGCCTGAACCTTTCACTGATACTGTGTTTTCTTGGTCCCTGGAGGACATTCTCAATGAGGATCTTTACAAAGACAAGGTGAAGGAGATTCCTAATTCATTTCAATCGGCTCAGCATTACTTTGGGTCTTATATATATCCTTTACTGGAAGAAACTCGAGCAGAAGTGCATTCAAGTATGGAAACTCTTAATAGAGCACCGTTTGCTGAAGTAGTGGGTTTTGAAGAATTGAAGCCATATGGAACAAAGCTATATGATGTCAAGGTTGATTACTGGCGAAACAGGTTCAGTGATAGAGGCAAGGAGCCATATAAAACTTTGCCTGGTGATCTTTTTGTTTTAGCAGATGCTAAACCTGAAACTGTTTCTGATTTACAAAGGGTGGGAAGGTCATGGGCTTTTGCATCGGCCACTAGTGTCTCAGAAAGTGAGAATGAAGATGACAGTACCTCTCTTTATTTTAAAGTCAAGGCATCGAAAGAGTTTGAAGTCGAAAATATCAGGACATCACTTTTTATAGTTTTCTTGGTTAATTTGATCCCTGAAGGAAGAGTTTGGAAAGCATTGCACATGTCCAGAAATCTGAAGATTATCAACGGAGTTCTGTGCACTGATTCAATGGTTCAGACAGATTTCTGCTCTGAAAAGAATAATAACATCTGGAATCAGAGTATAGCTGAGAGTCTATTATCTGGATTAAATAAGTCCCAAACTGGGGCAGTGTTGGCCTGCCTTGAAATGCTGCGTTATGGTGAGAAGTCTGCCGTTGAACTTATCTGGGGACCTCCTGGTACTGGCAAAACAAAAACCACGGTTACTCTGCTTTCAACCCTGCTACAGATGAAATATAGGACTCTTATCTGTGCTCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGTCTTGTGAAAATGGTGCATGAAGCAGAGTCAAAAGAATTGGTTTGTTCTCTAGGAGAGATTCTTCTATTTGGGAATAAGGAGCGACTCAAAATTGGTTCAGAGATTGAAGAAATTTATATGGATTGCCGTATCAAAAGGCTAGAAGATTGTTTAGGGCCAACGACTGGTTGGAGGCATTGCTTTGCTTCCATGATAGGTTTACTTGAGGATTGTGTTTCTGACTACCATATTTTCTTGGAAAATGAATTCACCAAAGAGACGGAACAAACAACTGTTAGTGAAATGAAAGATAATGAATGCAGAAGAGTTGCTCAAGTTAAAAAGTTGAAGTGCAAAACCTTTGTCGAATTTTTGAGAGATAGATTTGTCTCTACTGCAGTGCCGTTAAGATGTTGCATTTCTGTGTTCTGTACTCATATAGGGAAAAGTTATATTCCGGAAGATAATTTCCAAGACTTGGTTTCACTTGTTCAGAAAGTTGAATCCTTTAAGACAATGTTGTTGTCTCAAGATAATGTTGTTTCTGAAGCCTTGGAAGAACTCTTTTCCCATTCAGATGTTGAAGATGTGTCTAAGTCGTTAGTAGACAAGTTCTTTGATCTTTGGACGATGAGAAAGGATTGCCTTATAGCTTTACGCACTTTACGGGATTCTCTTAGTGAGCTTAAACTTCCAAATATTAGGAGCAAGGATTCTATACGGGAGTTCTGTTTTCAAAGAGCAACTCTAATATTTTGCACTGCATCTAGTTCATATAAGCTGCACAGAGTGGCAATGCAGCCACTAAGTATTGTTGTTATTGACGAGGCTGCACAGCTAAAAGAGTGTGAGTCAACAATACCCCTGCAACTTCCAGGTGTGAAGCATGCTGTTCTTGTTGGTGATGAATGTCAGTTACCAGCTACTGTAACAAGTAATGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGTTTAATGGGTCATTCAAAACATCTATTAAATGTGCAATACAGAATGCATCCATCAATAAGTTTATTCCCAAATTCAAGTTTCTATTCTAACTTGATCCTGGATGCTCCAAATGTAAAACGAAGAAGCCACGAGAAACACTATCTTCCAGGAGCAATGTTTGGTCCGTATTCTTTTATAAATGTGATTGGTGGAAGAGAGGAGAAAGATGAGGATGGACGTAGTCGGAAGAATATGGTGGAGGTTGCTGTTGTTTCAAAAATACTGCGGAATCTGTACAAAGGATGGGTTGAATCAAAACAGAGACTTAGTATTGGTATAGTATCTCCCTATGCTGCTCAAGTGGTGGCAATTAAGGACAGAGTGGGTGGGAAATATGATAAGCTTGATGGCTTTGTAGTGAAGGTAAAAACAGTTGATGGATTCCAAGGTGGGGAAGAGGACATAGTTATAATTTCCACTGTACGATCCAACAGCCATCAATCACTTCAGTTCATTTCAAAACCGCAAAGAGTTAATGTCTCACTTACAAGGGCTAGGCACTGTTTGTGGATTTTGGGGAATGAAAGGACTCTCTCTGATAGCGAATCTGTTTGGAGTGCTTTGGTCCTTGATGCCAAAAATCGTCATTGCTTCTTTCATGCTGATGAAGACAAGGATTTAGCCAAGGCCATATTAGAGGTAAAGAAAGAGTTTGACCAATTTGATGATTTGCTCAATGCCGACAGCATACTTTTTAGAAGTGCTAGATGGAAGGTACTTTTCAGTGATAACTTTCTGAAGTCATTCAAGAAACTGACATCAGTCCGCTTGCAGAAGTCAGTGCTACATCTTCTACTGAATCTTTCCAGTGGTTGGAGACCTAAGAAGCAAAATTTTGAGACAAGTTGTGGTAGCTCATCTATGATCTTGAAGAAATTTAAGGTTGAACGCTTATACATTGTTTGCACTAATGATATTGCAAAGAACTTGAGCTATGTTCAAGTATTAAAGATCTGGGACATATTGCCTCTGGAGGATATTCCAAAGTTGATAAACCGCCTGGATAGTATTTTCAACAGATATACTGATGACTTCATTAATCTTTGCAAGGAGAAGTGTCTTGATGGTGATCTAGAAGTTCCAAAAACCTGGCCACCCTCATTGCAATTTTCCCGGTTTAAGGATCTTAACATCAGTGAAGCTCAGAGTGATTTAGTTGGTGACACTTCTGATTGTAGAAGCTATGTTGAGAACTCACAGGTTAGCGAGAGTTTGTTACTGATGAAATTTTATTCCTTGTCGTCTGGGGTAGTGAACCACTTGTTGTCAGACCGTGAGGGTAGAGAGTTGGATCTTCCATTTGAAGTTACAGATGAAGAGATGGAGATTATCCTTTACCGTAGAAGTTCTTTTATAGTGGGACGGTCAGGAACTGGGAAAACCACAGTATTGACAATGAAATTGTTTCAAAAAGAACAGTGGCACCAATTTGCTGTTCAAGGATGCTATAATAGTCAAAATACTAGCCAGATTGAGGAGAGTTCTGCAGCTACTGAAGGGAAAGTTCTGCGCCAGCTTTTTGTGACAGTGAGTCCTAAGCTATGTTTTGCCATCAAGCGACATGTTTCACACTTGAAAAGTTTCGTATGTGGTGGAGGTAACTCAACTGACTCAAGTGAGGGAGGTTTGATTGATATGGTTGATTTTGATGAGGAGGAAACCCAATTCAAGGATATCCAAAGTTCATTTCACAATGTTCCTCCAAGTTCTTATCCTCTCGTCATTACATTCCATAAGTTTTTGATGATGCTCGATGGAACTTTGAGTAACTCATACTTTGAAAGATTCCTTGAAGTGACAGCTACTGTTGGCCAGTTTAGGAGTTCGTCATCAGTTGCATTTCAGAGCTTTATAAGGACGAAAGAAGTCAATTATGAGAGGTTTAGCTCTTTGTATTGGCCCCGTTTTAATGCTCAGATAACGAAGAAGCTTGACGTTTCAAGAGTCTTTATAGAGATTATTTCTTATATAAAAGGTGGCTTAGGAGCTATAGATGCATGTGATGGTAAACTCAGCCGGCAGGATTACATTCATCTGTGTGAGGGCCGTGGTTCTAACTTGAGCAAGCAAAAGAGAGAAGCAATATACGATATTTTTCAGGCTTATGAAAAGTTGAAGATGGAAAATGGTGAGTTTGATCTGGCTGATTTTGTAATAGATCTTCACTGTCGACTCAAGCAAGAAAAATATGAGGGTGATCAGATGGAATTTGTTTATATTGATGAGGTTCAAGATCTCACAATGAGTCAAATTGCTTTGTTTAAACATCTGTGCAACAATGTAGAAGAGGGATTTGTTTTTTCTGGTGATACAGCACAAACAATAGCAAGGGGTATTGACTTTCGGTTTCAGGATATACGACATCTGTTCTACAAGAAGTTTGTACTAGAGTCAAGAAGCAACAGTAACGATGAAAGAAAAGAAAAAGGCCTCATCTCTGAATTACTGCAGTTGACTCAGAACTTCCGTACTCATGCCGGTATATTGAAGTTATCACAAAGCATTGTTGAACTACTGTATCGGTTTTTCCCATTTTCTGTAGATGTTCTGAAACCTGAAACCAGTCTGATTTATGGGGAAGCTCCAGTTTTGCTTGAATCTGGAGAAAACGAAAATGCAATCATGAAAATTTTTGGAAACAGTGGAAATATAGTTGGTTTTGGTGCAGAGCAGGTGATTTTGGTGCGAGATGATAGTGCCCGACAAGAAATATCCAAATTTGTAGGGAAGCATGCTCTTGTACTAACCATAGTGGAGTGTAAGGGCCTTGAGTTTCAGGATGTCCTCGTATACAACTTTTTCGGTTCATCACCTTTGAAAAATCAGTGGAGGGTTATCTATGATTACATGAAGGAACAAGATTTACTTGACTCCACATTACCTAAGGGCTTTCCAAGTTTCAGTGAATCAAAACATAACATACTGTGCTCTGAACTTAAGCAATTATATGTTGCTGTTACTCGAACAAGACAGAGGTTGTGGATTTGTGAAAATGTGGAAGACCTCTCAAAACCTATGTTTGACTATTGGAAGAAAAAATGTCTTGTGCAACAAAGGCAGCTAGATGATTCACTTGCCCAGGCTATGCAAGTGGCAAGCAGTCCAGAGGAGTGGAAATCAAGAGGCATTAAGCTATATCATGAACATAACTATGAAATGGCCACAATGTGCTTTGAAAGAGCTGGTGATATGTACTGGGAAAGGAGGTCGAAAGCATCCGGCCTTAAAGCTATAGCTGACCGTATGCGAACTTCAAATCCTGAAGAGGCTAAGTCTATTCTTAGAGAAGCTGCTGAAATATTTGACGCTATAGGCAAGGCGGATTCTGCTGCCAGATGTTTTTCTGATTTGGGGGAATATGAAAGAGCAGCTAGGATATATTTCGAAAAATGTGGAGAATCTGAACTTCAGAGAGCTGCAGAGTGCTTTTCACTAGCGGGATGTTATGATCGTGCTGCAGAAGTCTATGCCAAGGGCAATTTTTTCTCAGAGTGTCTCACTGTTTGTGCGAATGGGAAACTTTTTGAAATGGGTTTGGATTATATTAATTATTGGAAACAGCATGCTGTTGAGGACTGTGCTAAGGCTAGTAGAGGAGAAGGAATAGATAAAATGGAGCAGGAATTCTTAGAGAGCTGCGCACTTCATTATCACAAGTTGAAAGATAACAGATCCATGATGAAATTTGTGAAAGCTTTCAATTCTGTAATAATGATGCGGGACTTTCTGAAAAGTTTAGGGAGCCTTGATGAGCTTTTGTCATTGGAGGAAGAATTTGGAAATTATTTGGAAGCAGCAAACATTGCCCAGCTTAAGGGTGACATTCTTCTTGAATCTGATTTCCTTGGAAAGGCAGGCAAGTTCAAAGAGGCCTCACTGCATATTCTGTTATATGTATTTTCCAAGTCTCTTTGGTCATCTGGTAGCAAAGGCTGGCCCATGAAGAAGTTTCCAAGACAGGAGGAGCTGTTGATGAAGGCCAAGTCATTTGCCAAGAATGGGGAAGATAATGTTTATAAGCTGGTTTGCACCGAGTCGGAGATTTTACTGAATGGGCAGGATAATTTGGCTGGTTTGAATCATCACATGGATGCTTCTCAAAGACATAAAAGTATTATAGGTGAAGTGTTGTCAGCTCGAAAAATTTTGGATGCACATCTCTCTTCAAGAACTGACAAGTATTTTTGGGAGAAAGAATTGACTGGAGATGTTGTGAAGCATTCAAAAGACTGTATATCTAGAAATCAGGTATCCATTGATTCGCTTGCCTATTACTGGATTTTATGGAAGGAGAACATTCTCCACATTATTGAATCTGTAGGATGTTTGGAAACTCCAGAATTCAATAGCTATGTAGAGCTCTGTTTTACTTGTTTAGGAGTTTGGCGGTTGTATCAGAATCTGAATCCAATCTATGTTTTACTGGTCCCTGATGCTGATTGGGTCAGAGGTCTGGACAAAAGGCATTTGAAAACTTATAATAAGTTGGTCTCCATTGATGCTCACCAGCTCATTCCAGCCGCTCAGAATTATTGGAGTTCAGAACTGCTCTCTGTTGGCATCAAAGTTTTGGAAAAGGTTGAAGCACTTTTCAAGTTCCCACCAAAGAACTCTGACTCAACATTCTTCCAGTGCAGGGCTCTTACCCTTATTTATGATGTTGCAAGGAATCTTTTGGATTCGAAGTGTCTGAAGCCATGGTATCAAGATATGTCGACCCTGCAGAAGCATGTAACATTTTCAACTGGGGAGTATGTTGCTTACATCTTTCCTTTAGATGGGAAGAATTTGTTGAGACAAAACATGATTTCTCTTAGAAGAACTGATGCTTCCAAGAACATGCTGAATCAAGTTATAGTTGAATATGCCAGCTCGAAGAATGAGCTGTCTTATGGCCAGATTGGAAGGATCACAATGATCATTCTCGGGTTAGGTAAGCCGAATGCTGAGCTCTCTGATAAGCTTGTGAAAAAACTTGAATGCCATGCCCCATGGAAGGCCTTCATTGAGAATCTATGTGTGAGCACTGAACCTGGAAATACCAGTCTGCAACCAATGAAGTTGTCTGTCATGCGTTGGTTACATGAGGCTTTGGTTGAAACTTACAGTGCCAACTGGAGGAAGGTTTCCGACTACATATCACCTGGTTGCTTCTTGTATCTTGTAGAGCGCCTTCTGCTCTCAGCAAGTTGCTTTCAAGGTTTGGTTATCAGTACAAGTTCTTGCTTTATCGAATGGCTGATATATCAGGATGAAGATACCAGCTTACGTTGCACAGTGACTGGTGGACGTCCTGCTCTGGGAGGCACTCTTCCATTTGTGATTGGTGTGGTTCGCGATTGTATTTACAACAAGGAGAATATGATGATTGAATGGATTAAGAGGTCTGACAAAAACTGGAGGAAATATTATCAGCCACTCATGTTGCGATTGGTTACTGTCTTATGCTTGATTAATTTGAACTTTGGCTCGTGTTTGGATATAATCCATGATTTGCTGGGGAGGAAATATATCACTGATCTCCTACCATGGGAATTTTTCGATGCACTTAGGAGGAGGAGGAAACCCAATTCTCTCAGCTTAAATGTGAATGTGCTTGCTGGAGCTCTCAAGAAGATTGATAATACTTTGGTAGTTGCAAGTTTTGGGCTTGATTGTTCAAGATTTTTCTGTTCAGATGCCATCTTTGTCGACATGACGGCCCACCAGTCCAAGGATGACATATTGAGAAAATGGTTTCCAAAGCTGCCTGTCGTTCAAGTTGCACCATCCCAAGTTGGATCTGTTGAAGTTGGAGGTAGTAGTTCTTGCAGTGGTGTACTATCTGCAAATGATTCTGAAGGAGAAAAGAGTTCTAAACTTTCTCCTTCAGACTCCAGCTTGGTAAAAGAGACTGATGTTGCAGATGAAACGCAATGTGAAGCTGAATTCAGTCACAGTAAAGATGAGGGTAATTGCATATCCAAAGTGTCAGCTGAGAAGCAGTGTGATGCCGAAGACAGCCACACAGAAGGCGAGGAAAACTGCGCATCCAGTGAGTCAAATCCGACATGCCAACAAGATGTCAATGCTTCCACCCCATCCGGCAGTAAAGAAGCTGGAAGCAAGGCCAAGAACAAGACAAAGGGCAAGAACAAGAAAAACAAGAAGAAAGGCGGGCGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2702

Amino Acids

307.01

Weight (kDa)

6.35

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 76 - 204 3.3e-65 Domain of unknown function (DUF6469)
AAA_11 PF13086 242 - 615 9e-36 AAA domain
AAA_19 PF13245 263 - 611 3e-08 AAA domain
AAA_12 PF13087 624 - 757 8.8e-28 AAA domain
UvrD-helicase PF00580 966 - 1071 6.1e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 2075, 2348, 3791, 5955, 6417
AarI CACCTGC 1 cut(s) 4600
AatII GACGTC 1 cut(s) 7231
Acc16I TGCGCA 2 cut(s) 5578, 7966
Acc36I ACCTGC 1 cut(s) 4600
AccB1I GGYRCC 1 cut(s) 3360
AccB7I CCANNNNNTGG 3 cut(s) 1097, 1390, 2751
AccBSI CCGCTC 1 cut(s) 6461
AccI GTMKAC 5 cut(s) 1518, 4108, 4728, 6653, 7629
AccII CGCG 1 cut(s) 7275
AccIII TCCGGA 1 cut(s) 1369
AclWI GGATC 9 cut(s) 99, 562, 1987, 2358, 3069, 3237, 5604, 6854, 7726
AcoI YGGCCR 5 cut(s) 438, 3029, 3748, 5097, 6832
AcyI GRCGYC 1 cut(s) 7228
AdeI CACNNNGTG 2 cut(s) 1702, 5106
AflII CTTAAG 2 cut(s) 4874, 5750
AflIII ACRYGT 2 cut(s) 600, 3499
AhdI GACNNNNNGTC 1 cut(s) 3468
AhlI ACTAGT 1 cut(s) 443
AjuI GAANNNNNNNTTGG 4 cut(s) 3357, 3389, 3609, 3641
AloI GAACNNNNNNTCC 2 cut(s) 5179, 5211
Alw21I GWGCWC 8 cut(s) 229, 641, 898, 4868, 6300, 6891, 6965, 7546
Alw44I GTGCAC 1 cut(s) 637
AlwI GGATC 9 cut(s) 99, 562, 1987, 2358, 3069, 3237, 5604, 6854, 7726
AlwNI CAGNNNCTG 6 cut(s) 1838, 3311, 3431, 3743, 6340, 7220
Ama87I CYCGRG 2 cut(s) 186, 6863
Aor13HI TCCGGA 1 cut(s) 1369
ApaLI GTGCAC 1 cut(s) 637
ArsI GACNNNNNNTTYG 4 cut(s) 1260, 1292, 1511, 1543
AseI ATTAAT 3 cut(s) 2976, 5484, 7392
Asp700I GAANNNNTTC 4 cut(s) 2681, 3518, 5633, 6938
AspLEI GCGC 4 cut(s) 3450, 5579, 7110, 7967
AsuC2I CCSGG 1 cut(s) 3052
AsuII TTCGAA 2 cut(s) 5322, 6620
AvaI CYCGRG 2 cut(s) 186, 6863
AvaII GGWCC 5 cut(s) 66, 812, 2060, 2503, 6364
AxyI CCTNAGG 1 cut(s) 4821
BaeGI GKGCMC 2 cut(s) 641, 4639
BaeI ACNNNNGTAYC 2 cut(s) 4439, 4472
BalI TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
BanI GGYRCC 1 cut(s) 3360
BanII GRGCYC 4 cut(s) 6300, 6580, 6891, 7546
BarI GAAGNNNNNNTAC 6 cut(s) 4083, 4115, 5173, 5205, 7095, 7127
BauI CACGAG 2 cut(s) 2027, 7408
BbsI GAAGAC 3 cut(s) 2553, 4944, 7941
Bbv12I GWGCWC 8 cut(s) 229, 641, 898, 4868, 6300, 6891, 6965, 7546
BceAI ACGGC 5 cut(s) 779, 1044, 1300, 3969, 7653
BciVI GTATCC 1 cut(s) 6199
BclI TGATCA 2 cut(s) 4135, 6855
BcnI CCSGG 1 cut(s) 3052
BcuI ACTAGT 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 7111
BfrI CTTAAG 2 cut(s) 4874, 5750
BfuAI ACCTGC 1 cut(s) 4600
BfuI GTATCC 1 cut(s) 6199
BglII AGATCT 3 cut(s) 2889, 4096, 4171
BlpI GCTNAGC 2 cut(s) 141, 6885
Bme18I GGWCC 5 cut(s) 66, 812, 2060, 2503, 6364
BmeRI GACNNNNNGTC 1 cut(s) 3468
BmeT110I CYCGRG 2 cut(s) 186, 6863
BmiI GGNNCC 7 cut(s) 68, 336, 813, 3362, 3839, 5681, 6366
BmrI ACTGGG 4 cut(s) 753, 3321, 5143, 6693
BmuI ACTGGG 4 cut(s) 753, 3321, 5143, 6693
BoxI GACNNNNGTC 1 cut(s) 2699
BpiI GAAGAC 3 cut(s) 2553, 4944, 7941
Bpu10I CCTNAGC 3 cut(s) 3474, 3919, 5520
Bpu1102I GCTNAGC 2 cut(s) 141, 6885
Bpu14I TTCGAA 2 cut(s) 5322, 6620
BpuMI CCSGG 1 cut(s) 3052
BsaBI GATNNNNATC 2 cut(s) 3006, 6093
BsaHI GRCGYC 1 cut(s) 7228
BsaI GGTCTC 2 cut(s) 2749, 6433
BsaWI WCCGGW 1 cut(s) 1369
BsaXI ACNNNNNCTCC 4 cut(s) 5891, 5921, 6135, 6165
Bse118I RCCGGY 2 cut(s) 3954, 4415
Bse21I CCTNAGG 1 cut(s) 4821
Bse3DI GCAATG 8 cut(s) 593, 1108, 1713, 2058, 2524, 3038, 5739, 7859
Bse8I GATNNNNATC 2 cut(s) 3006, 6093
BseAI TCCGGA 1 cut(s) 1369
BseJI GATNNNNATC 2 cut(s) 3006, 6093
BseMI GCAATG 8 cut(s) 593, 1108, 1713, 2058, 2524, 3038, 5739, 7859
BseRI GAGGAG 8 cut(s) 2111, 3428, 3605, 5060, 5547, 5915, 7507, 7510
BseSI GKGCMC 2 cut(s) 641, 4639
BseYI CCCAGC 2 cut(s) 5745, 7435
BsgI GTGCAG 4 cut(s) 1679, 1728, 4623, 6592
Bsh1236I CGCG 1 cut(s) 7275
BshNI GGYRCC 1 cut(s) 3360
BsiHKAI GWGCWC 8 cut(s) 229, 641, 898, 4868, 6300, 6891, 6965, 7546
BsiHKCI CYCGRG 2 cut(s) 186, 6863
BsiSI CCGG 6 cut(s) 1370, 3052, 3955, 4416, 5157, 8022
BslFI GGGAC 7 cut(s) 52, 721, 825, 2909, 3312, 5672, 6350
BsmBI CGTCTC 1 cut(s) 1184
BsmFI GGGAC 7 cut(s) 52, 721, 825, 2909, 3312, 5672, 6350
BsmI GAATGC 6 cut(s) 199, 1235, 1947, 6157, 6886, 6923
Bso31I GGTCTC 2 cut(s) 2749, 6433
BsoBI CYCGRG 2 cut(s) 186, 6863
Bsp119I TTCGAA 2 cut(s) 5322, 6620
Bsp13I TCCGGA 1 cut(s) 1369
Bsp1407I TGTACA 1 cut(s) 2172
Bsp1720I GCTNAGC 2 cut(s) 141, 6885
Bsp19I CCATGG 3 cut(s) 6635, 6929, 7466
Bsp68I TCGCGA 1 cut(s) 7275
BspEI TCCGGA 1 cut(s) 1369
BspFNI CGCG 1 cut(s) 7275
BspHI TCATGA 2 cut(s) 4560, 5077
BspLI GGNNCC 7 cut(s) 68, 336, 813, 3362, 3839, 5681, 6366
BspMAI CTGCAG 6 cut(s) 1312, 3427, 4389, 5359, 5398, 6663
BspMI ACCTGC 1 cut(s) 4600
BspPI GGATC 9 cut(s) 99, 562, 1987, 2358, 3069, 3237, 5604, 6854, 7726
BspT104I TTCGAA 2 cut(s) 5322, 6620
BspT107I GGYRCC 1 cut(s) 3360
BspTI CTTAAG 2 cut(s) 4874, 5750
BspTNI GGTCTC 2 cut(s) 2749, 6433
BsrBI CCGCTC 1 cut(s) 6461
BsrDI GCAATG 8 cut(s) 593, 1108, 1713, 2058, 2524, 3038, 5739, 7859
BsrFI RCCGGY 2 cut(s) 3954, 4415
BsrGI TGTACA 1 cut(s) 2172
BssAI RCCGGY 2 cut(s) 3954, 4415
BssNAI GTATAC 1 cut(s) 4729
BssNI GRCGYC 1 cut(s) 7228
BssSI CACGAG 2 cut(s) 2027, 7408
BssT1I CCWWGG 9 cut(s) 1464, 2323, 2562, 5409, 5782, 6635, 6929, 7466, 7650
Bst1107I GTATAC 1 cut(s) 4729
Bst2BI CACGAG 2 cut(s) 2027, 7408
Bst6I CTCTTC 9 cut(s) 574, 1231, 2086, 2328, 3249, 4218, 5196, 6106, 7254
BstACI GRCGYC 1 cut(s) 7228
BstAFI CTTAAG 2 cut(s) 4874, 5750
BstAPI GCANNNNNTGC 2 cut(s) 233, 5741
BstAUI TGTACA 1 cut(s) 2172
BstBI TTCGAA 2 cut(s) 5322, 6620
BstDSI CCRYGG 5 cut(s) 840, 3985, 6635, 6929, 7466
BstENI CCTNNNNNAGG 1 cut(s) 5786
BstFNI CGCG 1 cut(s) 7275
BstH2I RGCGCY 1 cut(s) 7111
BstHHI GCGC 4 cut(s) 3450, 5579, 7110, 7967
BstMWI GCNNNNNNNGC 9 cut(s) 233, 755, 1871, 3932, 4993, 5264, 5741, 7114, 7209
BstNSI RCATGY 9 cut(s) 604, 1802, 3503, 3939, 4673, 5504, 6671, 6781, 7992
BstPAI GACNNNNGTC 1 cut(s) 2699
BstSLI GKGCMC 2 cut(s) 641, 4639
BstUI CGCG 1 cut(s) 7275
BstV2I GAAGAC 3 cut(s) 2553, 4944, 7941
BstX2I RGATCY 8 cut(s) 91, 2889, 3061, 3229, 4096, 4171, 5609, 7718
BstXI CCANNNNNNTGG 2 cut(s) 3027, 5049
BstYI RGATCY 8 cut(s) 91, 2889, 3061, 3229, 4096, 4171, 5609, 7718
BstZ17I GTATAC 1 cut(s) 4729
Bsu36I CCTNAGG 1 cut(s) 4821
BsuI GTATCC 1 cut(s) 6199
BtgI CCRYGG 5 cut(s) 840, 3985, 6635, 6929, 7466
BtsI GCAGTG 6 cut(s) 756, 1317, 1671, 5813, 7756, 7928
BtuMI TCGCGA 1 cut(s) 7275
BveI ACCTGC 1 cut(s) 4600
CaiI CAGNNNCTG 6 cut(s) 1838, 3311, 3431, 3743, 6340, 7220
CciI TCATGA 2 cut(s) 4560, 5077
CfoI GCGC 4 cut(s) 3450, 5579, 7110, 7967
Cfr10I RCCGGY 2 cut(s) 3954, 4415
CseI GACGC 1 cut(s) 5252
CsiI ACCWGGT 1 cut(s) 7081
CspCI CAANNNNNGTGG 4 cut(s) 3021, 3056, 7344, 7379
DraI TTTAAA 2 cut(s) 493, 4201
DraIII CACNNNGTG 2 cut(s) 1702, 5106
DriI GACNNNNNGTC 1 cut(s) 3468
EaeI YGGCCR 5 cut(s) 438, 3029, 3748, 5097, 6832
Eam1104I CTCTTC 9 cut(s) 574, 1231, 2086, 2328, 3249, 4218, 5196, 6106, 7254
Eam1105I GACNNNNNGTC 1 cut(s) 3468
EarI CTCTTC 9 cut(s) 574, 1231, 2086, 2328, 3249, 4218, 5196, 6106, 7254
EciI GGCGGA 1 cut(s) 5273
Ecl136II GAGCTC 3 cut(s) 6298, 6889, 7544
Eco130I CCWWGG 9 cut(s) 1464, 2323, 2562, 5409, 5782, 6635, 6929, 7466, 7650
Eco147I AGGCCT 3 cut(s) 4368, 5810, 6938
Eco24I GRGCYC 4 cut(s) 6300, 6580, 6891, 7546
Eco31I GGTCTC 2 cut(s) 2749, 6433
Eco32I GATATC 1 cut(s) 3613
Eco47I GGWCC 5 cut(s) 66, 812, 2060, 2503, 6364
Eco53kI GAGCTC 3 cut(s) 6298, 6889, 7544
Eco81I CCTNAGG 1 cut(s) 4821
Eco88I CYCGRG 2 cut(s) 186, 6863
EcoICRI GAGCTC 3 cut(s) 6298, 6889, 7544
EcoNI CCTNNNNNAGG 1 cut(s) 5786
EcoO109I RGGNCCY 2 cut(s) 812, 4702
EcoRI GAATTC 4 cut(s) 1178, 5561, 6278, 7865
EcoRV GATATC 1 cut(s) 3613
EcoT14I CCWWGG 9 cut(s) 1464, 2323, 2562, 5409, 5782, 6635, 6929, 7466, 7650
EcoT22I ATGCAT 2 cut(s) 1947, 3937
EcoT38I GRGCYC 4 cut(s) 6300, 6580, 6891, 7546
ErhI CCWWGG 9 cut(s) 1464, 2323, 2562, 5409, 5782, 6635, 6929, 7466, 7650
Esp3I CGTCTC 1 cut(s) 1184
FalI AAGNNNNNCTT 2 cut(s) 4788, 4820
FaqI GGGAC 7 cut(s) 52, 721, 825, 2909, 3312, 5672, 6350
FauI CCCGC 3 cut(s) 5368, 5649, 8087
FauNDI CATATG 1 cut(s) 268
FbaI TGATCA 2 cut(s) 4135, 6855
FblI GTMKAC 5 cut(s) 1518, 4108, 4728, 6653, 7629
FriOI GRGCYC 4 cut(s) 6300, 6580, 6891, 7546
FspI TGCGCA 2 cut(s) 5578, 7966
GlaI GCGC 4 cut(s) 3449, 5578, 7109, 7966
GsaI CCCAGC 2 cut(s) 5749, 7439
HaeII RGCGCY 1 cut(s) 7111
HapII CCGG 6 cut(s) 1370, 3052, 3955, 4416, 5157, 8022
HgaI GACGC 1 cut(s) 5252
HhaI GCGC 4 cut(s) 3450, 5579, 7110, 7967
Hin1I GRCGYC 1 cut(s) 7228
Hin6I GCGC 4 cut(s) 3448, 5577, 7108, 7965
HinP1I GCGC 4 cut(s) 3448, 5577, 7108, 7965
HincII GTYRAC 5 cut(s) 1767, 4109, 4392, 6654, 7630
HindII GTYRAC 5 cut(s) 1767, 4109, 4392, 6654, 7630
HindIII AAGCTT 5 cut(s) 1879, 2279, 3866, 5631, 6899
HpaII CCGG 6 cut(s) 1370, 3052, 3955, 4416, 5157, 8022
HpyCH4IV ACGT 4 cut(s) 2116, 3873, 7207, 7228
HpyF10VI GCNNNNNNNGC 9 cut(s) 233, 755, 1871, 3932, 4993, 5264, 5741, 7114, 7209
HpySE526I ACGT 4 cut(s) 2116, 3873, 7207, 7228
Hsp92I GRCGYC 1 cut(s) 7228
HspAI GCGC 4 cut(s) 3448, 5577, 7108, 7965
Kpn2I TCCGGA 1 cut(s) 1369
KroI GCCGGC 1 cut(s) 3954
KroNI GCCGGC 1 cut(s) 3956
Ksp22I TGATCA 2 cut(s) 4135, 6855
MabI ACCWGGT 1 cut(s) 7081
MaeII ACGT 4 cut(s) 2116, 3873, 7207, 7228
MbiI CCGCTC 1 cut(s) 6461
MflI RGATCY 8 cut(s) 91, 2889, 3061, 3229, 4096, 4171, 5609, 7718
MlsI TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
MluNI TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
Mox20I TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
Mph1103I ATGCAT 2 cut(s) 1947, 3937
MroI TCCGGA 1 cut(s) 1369
MroNI GCCGGC 1 cut(s) 3954
MroXI GAANNNNTTC 4 cut(s) 2681, 3518, 5633, 6938
MscI TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
MslI CAYNNNNRTG 6 cut(s) 1334, 1488, 2541, 3975, 5088, 6027
Msp20I TGGCCA 4 cut(s) 3031, 3750, 5099, 6834
MspA1I CMGCKG 1 cut(s) 7913
MspCI CTTAAG 2 cut(s) 4874, 5750
MspI CCGG 6 cut(s) 1370, 3052, 3955, 4416, 5157, 8022
MssI GTTTAAAC 1 cut(s) 4201
Mva1269I GAATGC 6 cut(s) 199, 1235, 1947, 6157, 6886, 6923
MvnI CGCG 1 cut(s) 7275
MwoI GCNNNNNNNGC 9 cut(s) 233, 755, 1871, 3932, 4993, 5264, 5741, 7114, 7209
NaeI GCCGGC 1 cut(s) 3956
NciI CCSGG 1 cut(s) 3052
NcoI CCATGG 3 cut(s) 6635, 6929, 7466
NdeI CATATG 1 cut(s) 268
NgoMIV GCCGGC 1 cut(s) 3954
NlaIV GGNNCC 7 cut(s) 68, 336, 813, 3362, 3839, 5681, 6366
NmuCI GTSAC 6 cut(s) 3098, 3460, 3733, 5756, 7216, 7871
NruI TCGCGA 1 cut(s) 7275
NsbI TGCGCA 2 cut(s) 5578, 7966
NsiI ATGCAT 2 cut(s) 1947, 3937
NspI RCATGY 9 cut(s) 604, 1802, 3503, 3939, 4673, 5504, 6671, 6781, 7992
NspV TTCGAA 2 cut(s) 5322, 6620
PaeI GCATGC 3 cut(s) 1802, 4673, 5504
PaeR7I CTCGAG 1 cut(s) 186
PagI TCATGA 2 cut(s) 4560, 5077
PaqCI CACCTGC 1 cut(s) 4600
PceI AGGCCT 3 cut(s) 4368, 5810, 6938
PciI ACATGT 2 cut(s) 600, 3499
PctI GAATGC 6 cut(s) 199, 1235, 1947, 6157, 6886, 6923
PdiI GCCGGC 1 cut(s) 3956
PdmI GAANNNNTTC 4 cut(s) 2681, 3518, 5633, 6938
PflFI GACNNNGTC 1 cut(s) 2118
PflMI CCANNNNNTGG 3 cut(s) 1097, 1390, 2751
PfoI TCCNGGA 2 cut(s) 1995, 2044
PmeI GTTTAAAC 1 cut(s) 4201
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 2 cut(s) 600, 3499
PshAI GACNNNNGTC 1 cut(s) 2699
PshBI ATTAAT 3 cut(s) 2976, 5484, 7392
PsiI TTATAA 5 cut(s) 2075, 2348, 3791, 5955, 6417
Psp124BI GAGCTC 3 cut(s) 6300, 6891, 7546
Psp5II RGGWCCY 1 cut(s) 812
PspFI CCCAGC 2 cut(s) 5745, 7435
PspN4I GGNNCC 7 cut(s) 68, 336, 813, 3362, 3839, 5681, 6366
PspPPI RGGWCCY 1 cut(s) 812
PspXI VCTCGAGB 1 cut(s) 186
PsrI GAACNNNNNNTAC 2 cut(s) 4785, 4817
PstI CTGCAG 6 cut(s) 1312, 3427, 4389, 5359, 5398, 6663
PstNI CAGNNNCTG 6 cut(s) 1838, 3311, 3431, 3743, 6340, 7220
PsuI RGATCY 8 cut(s) 91, 2889, 3061, 3229, 4096, 4171, 5609, 7718
PsyI GACNNNGTC 1 cut(s) 2118
PvuII CAGCTG 1 cut(s) 7913
RruI TCGCGA 1 cut(s) 7275
RseI CAYNNNNRTG 6 cut(s) 1334, 1488, 2541, 3975, 5088, 6027
SacI GAGCTC 3 cut(s) 6300, 6891, 7546
SalI GTCGAC 3 cut(s) 4107, 6652, 7628
SexAI ACCWGGT 1 cut(s) 7081
Sfr274I CTCGAG 1 cut(s) 186
SfuI TTCGAA 2 cut(s) 5322, 6620
SinI GGWCC 5 cut(s) 66, 812, 2060, 2503, 6364
SlaI CTCGAG 1 cut(s) 186
SmiMI CAYNNNNRTG 6 cut(s) 1334, 1488, 2541, 3975, 5088, 6027
SpeI ACTAGT 1 cut(s) 443
SphI GCATGC 3 cut(s) 1802, 4673, 5504
SseBI AGGCCT 3 cut(s) 4368, 5810, 6938
SspI AATATT 4 cut(s) 1612, 1665, 5238, 7346
SstI GAGCTC 3 cut(s) 6300, 6891, 7546
StuI AGGCCT 3 cut(s) 4368, 5810, 6938
StyI CCWWGG 9 cut(s) 1464, 2323, 2562, 5409, 5782, 6635, 6929, 7466, 7650
TaiI ACGT 4 cut(s) 2119, 3876, 7210, 7231
TatI WGTACW 7 cut(s) 1343, 2172, 4321, 4678, 5130, 7151, 7756
TauI GCSGC 1 cut(s) 6461
TseFI GTSAC 6 cut(s) 3098, 3460, 3733, 5756, 7216, 7871
Tsp45I GTSAC 6 cut(s) 3098, 3460, 3733, 5756, 7216, 7871
TspGWI ACGGA 4 cut(s) 642, 1208, 2052, 4394
Tth111I GACNNNGTC 1 cut(s) 2118
Van91I CCANNNNNTGG 3 cut(s) 1097, 1390, 2751
Vha464I CTTAAG 2 cut(s) 4874, 5750
VneI GTGCAC 1 cut(s) 637
VpaK11BI GGWCC 5 cut(s) 66, 812, 2060, 2503, 6364
VspI ATTAAT 3 cut(s) 2976, 5484, 7392
XagI CCTNNNNNAGG 1 cut(s) 5786
XbaI TCTAGA 2 cut(s) 3010, 6176
XceI RCATGY 9 cut(s) 604, 1802, 3503, 3939, 4673, 5504, 6671, 6781, 7992
XcmI CCANNNNNNNNNTGG 1 cut(s) 1098
XhoI CTCGAG 1 cut(s) 186
XmiI GTMKAC 5 cut(s) 1518, 4108, 4728, 6653, 7629
XmnI GAANNNNTTC 4 cut(s) 2681, 3518, 5633, 6938
ZraI GACGTC 1 cut(s) 7229
Zsp2I ATGCAT 2 cut(s) 1947, 3937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.