MD16G1270700.v1.1

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000223
Physical Location & Seq
Reverse (-)
35134023 .. 35149866
15844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1270700.v1.1.491

Sequence Viewer

Length: 7767 bp
ATGAGTGAGACAATGTCTGCTTCCGACCGTTTCATGGATACTGTGTTTTCTTGGTCACTTGAAGACATTTTCAATGAGAAACTGTACATAAACCAGTCGAGTATGGAAACTATTGACAGAGCACCATTTGCTAAAGTAATTGCTTTTGAAGAATCCGAGTCCAATTTAGATGATACAAGGACATATAGTATCATGGTTGATAAGTGGCAAAACAGGCAAAGAGAACGTGGAAAGGAACCATACAAAACTTTGCCTGGGGATCTTTTTGTCTTAGCAGATGCGAAACCCGAAACTGATTCTGATTTACGACGGGCAGGGAGGTGGTGGACTTTTGTATCAGTCACTAAGATCACCGAAAATAAAATAGAAGATGATATTAATGATGAGAAAATAGAGACTGAAATAAAAGAGTTTAAAGTCAAGGCATCAAAAAGGATTGAGGTCAAGGATAACATGAGCACTCCGCCATCACTATTTGTTGTTTTACTTGTGAATTTGATCACTAATCGCAGAATATGGGAAGCATTGCACATGTTCAGAAATCTGAAGATTATCAAGGATGTTCTGTGCACTGATTCTGTGGCTCAGAAAAACTACCCTCCCTCATATGAAATGGATGCTGACATTCGGGATAAGGTGATAGTTGAGAGTTCATCTTTTGGATTGAATGAGTCACAAACTGGGGCAGTTTTGGCCTGTCTTGAAATGTTGTTTTGTGATTCCAAGTCAACTTTGCAACTTATATGGGGTCCACCTGGGACAGGGAAAACTAAATCCACCGCTACTCTGCTGTTGACCCTGTTAAGGATGAATTGTAGGACTCTTGCTTGTGCCCCAACAAATTTTGCAGTAACTGAAGTAGCTTCCCATCTTGTAGAGATGGTGGCTAAAGCAGATTCCAATGATTGGTTTTATCCTCTGGGAAAAATTCTTTTATTTGGGAATAAAGAGGGACTCAAAGTTGGTTCTGATATTGAAGATATATATTTGGATCACCGTGTCGAAAGTCTTTTCGAGTGCCTGGGGCCGGTGACAGGTTGGAGTAGTTGCTCGTCTTCCATGATTAGGCTTCTTGAGGATTGCGTTTCACATTACCATATTTTCTTGAAAAATGAGTTCGCCAACGAGAAAAAACAAAATGGTGATGGTGAAATGATAGAGAAAGGATGTGGAAGAGAGACCGAAGTTAGCAAGGGCAAGTGCAAATCATTTCTTGAATTTTTCAGAGAGAGATTTGTGTCTACTGCATTACCCTTAAAATGTTGTATTTCTACCTTCTGTACCCATATAAGCCAAGATTACATTTCGGCACGTATTTTCCATGACATGATTTCGCTTTTTGGGTTAGTTGATTCCTTTAGAATACTATTGTTGCAAGGTAATGTTGATTCTGAAGCACTGGAAGAGCTTTTCTCTCGTTCAGAAGTTGAAGATGTGCTCGAGCCAGTAGACAACAACACATTTCATCTGTGCATGAAGAGAAGAGAGTGCCTTTCAGTTTTACGTACTCTTCAGAAATCGATTAGAAGACTTTACCTCCCAGATGTTAGGAACAAAAAATCTGTAATGGAGTTTTGTTTTCAAAGAGCTTCCTTAATATTTTGCACTGCATCTAGTTCGTTTAAGCTGCATAGAGTGAAATTGGAGCCACTGACCATTGTTGTTATTGATGAGGCTGCACAACTGAAAGAGTGCGAGTCAACGATACCCCTGCAACTTCCAGGTGTGAAGCATGCCGTTCTTGTTGGTGATAAGTGTCAGTTACCAGCTATGGTGAAAAGCAATGTGTCCTATAAAGCTGGTTTTGGAAGAAGCTTGTTCGAGAGGTTGAGCTCAATGGGTCACTCTAAACACCTTCTGAACACGCAATACAGAATGCATCCTTCAATAAGTTCCTTCCCAAATTCAAGGTTCTACAATAACCAGATCCTGGACGCTCCAAATGTTAAAAGAAGAAGCCATGAGAAACGCTATCTTCCAGGGCCAATGTTCGGTCCTTATTCTTTTATTAATGTAATTGATGGAAGAGAGGAGAAGGACGAGGACGAACATAGTCTAAAGAACATGGTAGAGGTTGCAATTGCTTTGAAAATACTGCAGAATTTGTACGAAAGATGGGTTGTTTCAAAAGAGAAGCTCAGTATTGGTGTAATATCTCCTTATGCAGCTCAAGTAGCTGCAATTCAGGAAAAACTTGGACAGAAGTACGATAAGCGTGATGGCTTTAAAGTGAAGGTGAAGACAGTTGATGGGTTCCAGGGTGGTGAAGAGGACATAATCATCATGTCCACTGTGCGATCCAATCAACATCACTCACTCGGTTTTATATCGGAACCACAAAGAGTTAATGTTGCTCTCACTAGGGCTCGGCACTGCCTTTGGATTTTGGGAAATGAGAGAACTCTTTGTGGTAGCGAATCTGTTTGGGAGGCTTTGGTGCTTGATGCCAAGAGTCGGCAGTGTTTCTTTAATGCTGATGCAGACAAGGATATGGCCAAGGCCATATTAGAGGCGAAGAAAGCATTTCACCAATTTGATGATTTGCTTAATGCTGACAGCTTACTTTTCCGAAGTTGTAGATGGAAGGTACTTTTGAGTGGTAATTTTCTGGAGTCGTTCAGAAAACTTAAGTCGATTCAGTTGAAGAAGTCCGTGATAAATCTTCTACTGAAGCTTGCCGGTGGATGGAGACCTAAAAAGATAAATGTCCAGACTATTTCTGGCTCATCATCATTCTTGAGGCAATATAAAGTTAGAGGTCTATATGTTGTTTGCGCAATCGACATTGCAAAGGAGTTGGAATACACTCAAGTGTTAAGGATTTGGGACATATTGCCTCTAGAGTACATCCCAAAATTGATAAATCGTTTGGATAGGATTTTCAAGAGATATACAGATGACTTTATCAATCTCTGCAATGAGAAATGTCTTGAGGGTAACTTGGAAGTTCCAAAGTGCTGGCCGACCTCTTTGGATGTGCCTCAGTTTAAGGATCTTAGTGCCACTGAAATCGATAGTGATTTTGTTAGTGATGCTTCTGATGGTAGAAGTTATGTTGAGAATTCACAGGTCAGCGAGAGTTTGTTGCTCATGAAGTTTTACGCCTTGTCATCTGGTGTAGTGAATCACTTGCTGTCTGATCGTGAGGGTAAAGAGTTGGATCTTCCATTTGAAGTAACTGACCAGGAAATGGACATTATCCTTTATCGTAAAAGTACCTTTATAGTTGGACGATCAGGAACAGGTAAGACAACTGTTTTAACCATGAAATTATTTCAAAACGAACGATGTCACCATTTGGCACATAAAGGATGCGTTAGAAGTTCAAATAGCATGGTTGAGCCGAGTTCTTCGGCTACTCAGGAGAGAACTCTACACCAGCTGTTTGTTACCGTGAGTCCTCAGCTATGTTTTGCCATAAAACAACATGTTCTACACTTGAAAAGATTTGCATGTGGTGGAAGTGATTCAACGGAGAAAATTTTGATTGATATGGCTGATTTTGATGAGGAGGAATTTCAATTCAAGGACATCAAGGATTCGTTTCAGGATATTCCTCCCGATTCTTACCCTCTTGTAATAACATTTCATAAGTTCTTGATGATGCTGGATGGAACACTGACTAACTCATACTTTGAAAGATTCCTTGATGCAACAACGCTAACTCACGGTCAATTGAGAAGCTCAAGATCAGTTGCATTGCAAACCTTCATCAGGACAAAGGAGGTTAATTATGAGAGGTTCAGTTCATCTTACTGGCCCCATTTTAGTATGCAGTTAACAAAGAAGCTTGATGCTTCAAGAATCTTTACGGAGATTATATCTCATATTAAAGGTGGTTTAGCAGCCATAGAAGCAGGTGATGGCAAACTCAGTAGGGAGGATTATGTTCAACTGTCAGATGGCCGGGATTCCAATTTAAGCAAGCAAAAGAGAGAAGTAATATACGATATATTTCAGGCTTATGAAAAAATGAAGTTGAAGAATGGTGAATTTGATTTGGCTGATTTTGTCATTGATCTACACCATCGGATTAGGCACGAAAAATTTGTGGGTGATCAAATGGATTTTGTTTATATTGATGAAGTTCAAGATCTCACCATTAGCCAAATTGCGTTGTTTAAGCATATGTGCAACAATGTCGAAGAGGGTTTTGTTTTTTCCGGTGATACGGCACAGACCATTGCCAGGGGAATTGATTTTAGGTTCCAAGATATAAGACATCTGTTCTACAAGAAGTTTGTGTTGGAATCAAGAGGCAATAAGCATCAGGAAAGAAAAGAAAAGGGACAAATCTCAGAAACATTTCACTTGACTCAAAACTTCCGTACGCATGCTGGCGTTCTGAAATTATCACAGAGCATTGTTGAACTACTTTATTGTTTTTTCCCTCTATATATTGACATTTTAAGGCCTGAAACCAGTCTGATATATGGGGAAGCTCCAGTACTGCTTGAATCCGGAGAAGATGAAAATCCAATCATCAGAATTTTTGGAAATAGTGGAACTCATAATTCTAACATAGTTGGCTTCGGAGCTGAGCAAGTTATTTTGGTCCGTGATGTTGGTGCTCAAAAAGAAATTTTGAAAATCGTTGGGAAGCATGCTCTTGTCCTAACCATATTGGAATGCAAGGGGCTTGAATTCCAGGATGTACTCTTGTACAACTTTTTTGGATCATCGCCCCTAAAAAATCAATGGAGGGTGATATATGAGTACATGAAGGAGCAAGATTTACTTGACTCTACATTGCCACAACGATTTCCAAGTTTCAGTGAAGCTAAACACAACATCTTATGCTCTGAACTGAAACAATTATATGTTGCTGTTACTCGGACAAGACAAAGATTATGGTTTTGCGAGAATGTAGAAGAGCTCTCCAAGCCCATGTTTGACTATTGGAAGAAGAAATGTCTTGTACAGGTTAGACAACTGGACGATTCCCTTGTGCAGGCAATGCAAGCTGCTAGTAGTCCTGCGGCGTGGAAATCACGAGGCATTAAGCTCTATCATGAACATAACTACGAAAAGGCCACAAAGTGCTTTGAAAAAGCTGGTGATACTTATTGGGAAGGGAAGTCCAAAGCTGCTGGCCTTAAAGCTATCGCTGAGCGAATGCCGACTTCAAATCCTGCGGAGGCCAATACCATTCTTAAGGAAGCTGCTGAGATATATGATGCTATAGGCATGGCAGATTCCGCAGTTAGATGTTTTATTGATTTGGGAGAGTATGAAAGAGCAGCTAGGATTTATTTGGATAAATGTGGTGAACTGGAAAGAGCCGGGGAATGTTTTTCTCTAGCAGGATGCTATCAAGAGGCTGCTGATGTGTATGCTAAGGGCAATTTTTTCTCGGAGTGTCTCACTGTTTGTGACAAAGGAAAGCTATTTGAAATGGGTCTGAACTACATCAAATCTTGGAAGAAGCATCCGGTAAAGGACTCTGCTGTGGCTGAAAGGGGAGAAGGATCTGATGAAATTGAGCAGGCATTTCTCGAGAGATGCGCACTTTACTATTACGAGTTGAAAGACAACAGATCCATGATGAATTTTGTTAGAGCTTTTCATTCTATAATTTCCATGCGAAACTTTTTGAAGGAGCACGCTAGTCTTGATGAGCTTCTTTTGTTGGAAGAAGGATTGGGAAACTATCTTGAGGCTGCAGAAATCGCAAAACTAAAAGGCGATATTCTACTCCAAGCTGATTTCCTTGAAAAGGCTGGCAAGTTCAGAGAGGCATCATTGCACATTCTATTCTATGTGCTTGCCAACTCTCTTTGGTCATATGGCAGCAAGGGATGGCCCGTAAAGCAGTTTTCACAAAGGGAGGAGCTTTTATCAAAAGCCAAGTCATGTGCTAAGCACGAGACGGAAAGCTTTTACGACTTTGTTTGCACTGAGGTTGACATTTTGTTAATTGAGCAGAGTGACTTGGTTGTGATGAAAAATCACATGAATGCTTGCCAGAGACACAGGAGTGTTGGAGGTGAAATTTTATCTGCTAAAAAAATATTGGATGCTCATCTTTCTTCAAGCGGCGATGAGTATGTGTGGGAAGAAATGTTGGTTGATGGTCTGATAAAGTGTTCAGCAAACGAAATATCTGAAAATCAAGCTTCTGTGGACTCGTTGATTTATTTCTGGAATTTTTGGAAGGATAAAATTGCTCACATATTTGAATATCTCAGATGCCTCGAATACCAGGAAGTTAGTGAATACAAAAGTTATGGAGAGTTTTGTTTGAATTACTTGGGAGTTTGGAGAATGTATCATAATCTGAGTCCAGTCTATGTTTTACTGATTCCTGATGCTGATTGGGTTCATGGGTTGAATAAAAAATATTTTAGAAATCAAGGGGAATTGGTCTCAGTTGATGCTCAGCAGCTTGCTTCGGCCGCTAGGAATTATTGGAGTTCAGAATTGCTCTCTGTTGGCATGAAGGTTTTGGACAAGCTCGAAGAACTCTACAAGTTTCCCAGCAAGAATGTTGAGTCTGTGTTCCATCGAAGCAGGTGTCTTACCTATATCTGTGAGGTCTCGGTGTATCTTTTGCAATCAAAATGTTTGAACTTAAGGTATCATGATGCCGAGCGATTGCACAAATGTGTAACATTTTCGACTGAAAGTATTGTTCCTTTAGTATTTCCTATGGATTGGAGGAATTCATTGAGGGAGAATATGATTTCTCTTCGAAGAACTGATGCTTCAAAGAATGTGTTGAAACAAGTAATAAATGAGTTTACCAGAGAAAAGAATGAGCTATCTTTTGGGCAAGTTGGGAAGCTTGTAATGGTCATTCTCGGGACTGGTAAGCTTAATAATGATCTGTACAAGAAGCTTGTGAGAAAACTTGACTGTAACCTGACATGGCAGGCGTTCATCGAGAATCTCAGTGGGATTGTCGAACATGGAAACACCAGTCAAGAACCGAAAGAGGTGTCTGTAATGTTGAGGTTCCATGAAGCTTTAGCTGAAACTTATAATGTGAACTGGAGGGCTGTGAAGGATTACATAACACCTGGTTGCTTTCTATACCTTGTAGAGCGCCTTCTGATGTGGGCGACTTGCTTTCAGGGTTATGCTATCACTACAAGTTCTTGTTTTACCGAGTGGCTTATATACCAAGGGGAAGATACCTGCTTACGTTTGAAGGGGGCTGATGTTCGACCATCTTTGGATGGCATCCTTCATTTTGTGATTGATGTGGCTCGGGAGTGCATTTTCAATACGGCGGATATGGTTGAATGGATTGGTCGTTTTGTGATTCAAATGTCCATCGAAGATTGGAAGAACTACTATTCACTACTCATGTTCAGATTGGTCGTTATGTTATGCTTGGTTTATGTGAATTTTGGTATTGGTCTAGATTTACTCCATGATTTGCTGGGGAGGAACTATATCACTGAACAGCTGCCGTCGGAGTTCTATGATGCCCTCAAAAGCAGGAGGAGCCACAAATCTGTGAGGTTAGATGTGAATGTGCTCGCGGCAGCTCTTAAGAAGGTCGGTAATCCTTTGGTCATAGCACGTTTTGGGACCAATTGTTCAAGGTGGTTGTGTTCAGATGCTGTCCTTGTTGACATGATGGCCAACCGGTCTAGGGATGACGTATTTAGAAATTTGTTTCGTAAACCATCCGTCCTAAAAGCTTCGCCATCTGGATCTTGCAGTCGTGCGCTTTGTGCTACCAATTCTGAGGAGGGGAAGACTGGTGCTGCATTGGACAAGCCATGTGACGCCGGGGACAGCCACACTATATATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase UvrD_C

Protein Analysis

2589

Amino Acids

296.15

Weight (kDa)

6.34

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 42 - 182 2.6e-49 Domain of unknown function (DUF6469)
AAA_11 PF13086 222 - 595 1.2e-31 AAA domain
AAA_19 PF13245 241 - 591 2.3e-06 AAA domain
AAA_12 PF13087 603 - 799 2.5e-61 AAA domain
UvrD-helicase PF00580 1063 - 1395 6.3e-08 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6981
AarI CACCTGC 2 cut(s) 3848, 6501
Acc16I TGCGCA 2 cut(s) 2776, 5506
Acc36I ACCTGC 3 cut(s) 3848, 6501, 7145
AccB7I CCANNNNNTGG 3 cut(s) 906, 1930, 3190
AccI GTMKAC 2 cut(s) 1241, 1449
AccII CGCG 1 cut(s) 7487
AccIII TCCGGA 1 cut(s) 4460
AciI CCGC 9 cut(s) 464, 780, 4979, 5135, 5199, 6036, 6396, 7232, 7487
AcoI YGGCCR 5 cut(s) 2493, 2960, 3904, 6393, 7587
AcuI CTGAAG 5 cut(s) 566, 876, 1413, 1496, 2690
AcyI GRCGYC 1 cut(s) 7737
AdeI CACNNNGTG 1 cut(s) 5040
AflII CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
AflIII ACRYGT 2 cut(s) 531, 3427
AgeI ACCGGT 1 cut(s) 7593
AjuI GAANNNNNNNTTGG 2 cut(s) 4229, 4261
AleI CACNNNNGTG 3 cut(s) 2810, 5976, 6603
AloI GAACNNNNNNTCC 2 cut(s) 7528, 7560
Alw21I GWGCWC 9 cut(s) 124, 461, 572, 1440, 1835, 4573, 4878, 5604, 7485
Alw26I GTCTC 9 cut(s) 2, 389, 1172, 2683, 5366, 5861, 5962, 6370, 6541
Alw44I GTGCAC 1 cut(s) 568
AlwNI CAGNNNCTG 3 cut(s) 854, 1930, 7568
Ama87I CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
Aor13HI TCCGGA 1 cut(s) 4460
ApaLI GTGCAC 1 cut(s) 568
ArsI GACNNNNNNTTYG 6 cut(s) 5834, 5866, 6499, 6531, 6923, 6955
AseI ATTAAT 2 cut(s) 378, 2010
AsiGI ACCGGT 1 cut(s) 7593
Asp700I GAANNNNTTC 2 cut(s) 3466, 4305
AspLEI GCGC 4 cut(s) 2777, 5507, 7047, 7678
AspS9I GGNCC 8 cut(s) 749, 1025, 1982, 1994, 3759, 4555, 5802, 7536
AsuC2I CCSGG 3 cut(s) 3908, 5284, 7741
AsuII TTCGAA 1 cut(s) 6691
AvaI CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
AvaII GGWCC 4 cut(s) 749, 1994, 4555, 7536
BaeGI GKGCMC 2 cut(s) 572, 835
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BalI TGGCCA 2 cut(s) 2495, 7589
BanII GRGCYC 3 cut(s) 1835, 2368, 4878
BarI GAAGNNNNNNTAC 4 cut(s) 4431, 4463, 7032, 7064
BauI CACGAG 2 cut(s) 4992, 5864
BbsI GAAGAC 5 cut(s) 69, 1047, 1534, 2246, 7712
Bbv12I GWGCWC 9 cut(s) 124, 461, 572, 1440, 1835, 4573, 4878, 5604, 7485
BbvCI CCTCAGC 1 cut(s) 3402
BceAI ACGGC 4 cut(s) 1721, 4188, 7245, 7399
BcgI CGANNNNNNTGC 6 cut(s) 1599, 1633, 1693, 1727, 4123, 4157
BciVI GTATCC 1 cut(s) 31
BclI TGATCA 2 cut(s) 498, 4057
BcnI CCSGG 3 cut(s) 3908, 5284, 7741
BcoDI GTCTC 9 cut(s) 2, 389, 1172, 2683, 5366, 5861, 5962, 6370, 6541
BfmI CTRYAG 3 cut(s) 2096, 5181, 5661
BfoI RGCGCY 1 cut(s) 7048
BfrI CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
BfuAI ACCTGC 3 cut(s) 3848, 6501, 7145
BfuI GTATCC 1 cut(s) 31
BglII AGATCT 1 cut(s) 4093
BlpI GCTNAGC 4 cut(s) 4539, 5109, 5859, 6378
BmcAI AGTACT 1 cut(s) 4449
Bme18I GGWCC 4 cut(s) 749, 1994, 4555, 7536
BmeT110I CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
BmgT120I GGNCC 8 cut(s) 749, 1025, 1982, 1994, 3759, 4555, 5802, 7536
BmrI ACTGGG 1 cut(s) 690
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 5 cut(s) 69, 1047, 1534, 2246, 7712
BplI GAGNNNNNCTC 2 cut(s) 1397, 1429
BpmI CTGGAG 3 cut(s) 2630, 4428, 7012
Bpu10I CCTNAGC 2 cut(s) 3402, 5337
Bpu1102I GCTNAGC 4 cut(s) 4539, 5109, 5859, 6378
Bpu14I TTCGAA 1 cut(s) 6691
BpuEI CTTGAG 7 cut(s) 1094, 2154, 2758, 2793, 2951, 3670, 5675
BpuMI CCSGG 3 cut(s) 3908, 5284, 7741
Bsa29I ATCGAT 2 cut(s) 1520, 3012
BsaAI YACGTR 2 cut(s) 1313, 1505
BsaBI GATNNNNATC 2 cut(s) 4473, 6021
BsaHI GRCGYC 1 cut(s) 7737
BsaI GGTCTC 4 cut(s) 1172, 2683, 6370, 6541
BsaWI WCCGGW 4 cut(s) 4163, 4460, 5431, 7593
BsaXI ACNNNNNCTCC 2 cut(s) 1521, 1551
Bse118I RCCGGY 3 cut(s) 1027, 2678, 7593
Bse3DI GCAATG 9 cut(s) 524, 1789, 2784, 2923, 3698, 4182, 4748, 4962, 5741
Bse8I GATNNNNATC 2 cut(s) 4473, 6021
BseAI TCCGGA 1 cut(s) 4460
BseCI ATCGAT 2 cut(s) 1520, 3012
BseJI GATNNNNATC 2 cut(s) 4473, 6021
BseMI GCAATG 9 cut(s) 524, 1789, 2784, 2923, 3698, 4182, 4748, 4962, 5741
BseRI GAGGAG 5 cut(s) 2045, 3524, 5843, 7462, 7712
BseSI GKGCMC 2 cut(s) 572, 835
BseX3I CGGCCG 1 cut(s) 6393
BseYI CCCAGC 2 cut(s) 6476, 7384
BsgI GTGCAG 2 cut(s) 1662, 4970
Bsh1236I CGCG 1 cut(s) 7487
Bsh1285I CGRYCG 2 cut(s) 28, 6396
BshTI ACCGGT 1 cut(s) 7593
BshVI ATCGAT 2 cut(s) 1520, 3012
BsiEI CGRYCG 2 cut(s) 28, 6396
BsiHKAI GWGCWC 9 cut(s) 124, 461, 572, 1440, 1835, 4573, 4878, 5604, 7485
BsiHKCI CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
BsiSI CCGG 9 cut(s) 1028, 2679, 3907, 4164, 4461, 5283, 5432, 7594, 7740
BsiWI CGTACG 1 cut(s) 4328
BslFI GGGAC 7 cut(s) 772, 966, 2840, 4302, 6817, 7549, 7757
BsmAI GTCTC 9 cut(s) 2, 389, 1172, 2683, 5366, 5861, 5962, 6370, 6541
BsmBI CGTCTC 1 cut(s) 5861
BsmFI GGGAC 7 cut(s) 772, 966, 2840, 4302, 6817, 7549, 7757
BsmI GAATGC 4 cut(s) 1881, 4634, 5121, 5962
Bso31I GGTCTC 4 cut(s) 1172, 2683, 6370, 6541
BsoBI CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
Bsp119I TTCGAA 1 cut(s) 6691
Bsp13I TCCGGA 1 cut(s) 4460
Bsp1407I TGTACA 4 cut(s) 84, 4662, 4918, 6828
Bsp1720I GCTNAGC 4 cut(s) 4539, 5109, 5859, 6378
BspACI CCGC 9 cut(s) 464, 780, 4979, 5135, 5199, 6036, 6396, 7232, 7487
BspDI ATCGAT 2 cut(s) 1520, 3012
BspEI TCCGGA 1 cut(s) 4460
BspFNI CGCG 1 cut(s) 7487
BspHI TCATGA 3 cut(s) 3090, 5011, 6580
BspMAI CTGCAG 2 cut(s) 2100, 5665
BspMI ACCTGC 3 cut(s) 3848, 6501, 7145
BspQI GCTCTTC 2 cut(s) 1398, 4866
BspT104I TTCGAA 1 cut(s) 6691
BspTI CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
BspTNI GGTCTC 4 cut(s) 1172, 2683, 6370, 6541
BsrDI GCAATG 9 cut(s) 524, 1789, 2784, 2923, 3698, 4182, 4748, 4962, 5741
BsrFI RCCGGY 3 cut(s) 1027, 2678, 7593
BsrGI TGTACA 4 cut(s) 84, 4662, 4918, 6828
BssAI RCCGGY 3 cut(s) 1027, 2678, 7593
BssNI GRCGYC 1 cut(s) 7737
BssSI CACGAG 2 cut(s) 4992, 5864
BssT1I CCWWGG 2 cut(s) 2496, 7123
Bst2BI CACGAG 2 cut(s) 4992, 5864
BstACI GRCGYC 1 cut(s) 7737
BstAFI CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
BstAPI GCANNNNNTGC 2 cut(s) 128, 4957
BstAUI TGTACA 4 cut(s) 84, 4662, 4918, 6828
BstBAI YACGTR 2 cut(s) 1313, 1505
BstBI TTCGAA 1 cut(s) 6691
BstENI CCTNNNNNAGG 4 cut(s) 759, 3712, 4949, 5714
BstFNI CGCG 1 cut(s) 7487
BstH2I RGCGCY 1 cut(s) 7048
BstHHI GCGC 4 cut(s) 2777, 5507, 7047, 7678
BstMAI GTCTC 9 cut(s) 2, 389, 1172, 2683, 5366, 5861, 5962, 6370, 6541
BstMCI CGRYCG 2 cut(s) 28, 6396
BstNSI RCATGY 6 cut(s) 535, 1736, 3431, 3456, 4337, 4607
BstSFI CTRYAG 3 cut(s) 2096, 5181, 5661
BstSLI GKGCMC 2 cut(s) 572, 835
BstSNI TACGTA 1 cut(s) 1505
BstUI CGCG 1 cut(s) 7487
BstV2I GAAGAC 5 cut(s) 69, 1047, 1534, 2246, 7712
BstX2I RGATCY 8 cut(s) 259, 1926, 2992, 3160, 4093, 5468, 5537, 7661
BstXI CCANNNNNNTGG 1 cut(s) 2958
BstYI RGATCY 8 cut(s) 259, 1926, 2992, 3160, 4093, 5468, 5537, 7661
BstZI CGGCCG 1 cut(s) 6393
Bsu15I ATCGAT 2 cut(s) 1520, 3012
BsuI GTATCC 1 cut(s) 31
BsuTUI ATCGAT 2 cut(s) 1520, 3012
BtgZI GCGATG 2 cut(s) 4665, 6054
BtsI GCAGTG 3 cut(s) 1605, 2371, 2465
BveI ACCTGC 3 cut(s) 3848, 6501, 7145
CaiI CAGNNNCTG 3 cut(s) 854, 1930, 7568
CciI TCATGA 3 cut(s) 3090, 5011, 6580
CfoI GCGC 4 cut(s) 2777, 5507, 7047, 7678
Cfr10I RCCGGY 3 cut(s) 1027, 2678, 7593
Cfr13I GGNCC 8 cut(s) 749, 1025, 1982, 1994, 3759, 4555, 5802, 7536
ClaI ATCGAT 2 cut(s) 1520, 3012
CseI GACGC 2 cut(s) 1943, 7745
CsiI ACCWGGT 1 cut(s) 7018
CspAI ACCGGT 1 cut(s) 7593
DraI TTTAAA 2 cut(s) 415, 2227
DraIII CACNNNGTG 1 cut(s) 5040
EaeI YGGCCR 5 cut(s) 2493, 2960, 3904, 6393, 7587
EagI CGGCCG 1 cut(s) 6393
EciI GGCGGA 2 cut(s) 453, 7247
Ecl136II GAGCTC 2 cut(s) 1833, 4876
EclXI CGGCCG 1 cut(s) 6393
Eco105I TACGTA 1 cut(s) 1505
Eco130I CCWWGG 2 cut(s) 2496, 7123
Eco147I AGGCCT 1 cut(s) 4414
Eco24I GRGCYC 3 cut(s) 1835, 2368, 4878
Eco31I GGTCTC 4 cut(s) 1172, 2683, 6370, 6541
Eco47I GGWCC 4 cut(s) 749, 1994, 4555, 7536
Eco52I CGGCCG 1 cut(s) 6393
Eco53kI GAGCTC 2 cut(s) 1833, 4876
Eco57I CTGAAG 5 cut(s) 566, 876, 1413, 1496, 2690
Eco88I CYCGRG 4 cut(s) 1439, 5495, 6800, 7209
EcoICRI GAGCTC 2 cut(s) 1833, 4876
EcoNI CCTNNNNNAGG 4 cut(s) 759, 3712, 4949, 5714
EcoRI GAATTC 3 cut(s) 3061, 4643, 6661
EcoT14I CCWWGG 2 cut(s) 2496, 7123
EcoT22I ATGCAT 1 cut(s) 1881
EcoT38I GRGCYC 3 cut(s) 1835, 2368, 4878
ErhI CCWWGG 2 cut(s) 2496, 7123
Esp3I CGTCTC 1 cut(s) 5861
FalI AAGNNNNNCTT 2 cut(s) 4722, 4754
FaqI GGGAC 7 cut(s) 772, 966, 2840, 4302, 6817, 7549, 7757
FauNDI CATATG 3 cut(s) 607, 4128, 5785
FbaI TGATCA 2 cut(s) 498, 4057
FblI GTMKAC 2 cut(s) 1241, 1449
FriOI GRGCYC 3 cut(s) 1835, 2368, 4878
FspAI RTGCGCAY 1 cut(s) 5506
FspI TGCGCA 2 cut(s) 2776, 5506
GlaI GCGC 4 cut(s) 2776, 5506, 7046, 7677
GsaI CCCAGC 2 cut(s) 6480, 7388
GsuI CTGGAG 3 cut(s) 2630, 4428, 7012
HaeII RGCGCY 1 cut(s) 7048
HapII CCGG 9 cut(s) 1028, 2679, 3907, 4164, 4461, 5283, 5432, 7594, 7740
HgaI GACGC 2 cut(s) 1943, 7745
HhaI GCGC 4 cut(s) 2777, 5507, 7047, 7678
Hin1I GRCGYC 1 cut(s) 7737
Hin6I GCGC 4 cut(s) 2775, 5505, 7045, 7676
HinP1I GCGC 4 cut(s) 2775, 5505, 7045, 7676
HincII GTYRAC 6 cut(s) 729, 795, 1701, 3780, 5905, 7579
HindII GTYRAC 6 cut(s) 729, 795, 1701, 3780, 5905, 7579
HpaI GTTAAC 1 cut(s) 3780
HpaII CCGG 9 cut(s) 1028, 2679, 3907, 4164, 4461, 5283, 5432, 7594, 7740
Hpy99I CGWCG 2 cut(s) 312, 7420
HpyCH4IV ACGT 6 cut(s) 226, 1312, 1504, 7144, 7528, 7608
HpySE526I ACGT 6 cut(s) 226, 1312, 1504, 7144, 7528, 7608
Hsp92I GRCGYC 1 cut(s) 7737
HspAI GCGC 4 cut(s) 2775, 5505, 7045, 7676
Kpn2I TCCGGA 1 cut(s) 4460
Ksp22I TGATCA 2 cut(s) 498, 4057
KspAI GTTAAC 1 cut(s) 3780
LguI GCTCTTC 2 cut(s) 1398, 4866
LmnI GCTCC 8 cut(s) 1645, 1942, 4447, 4535, 4726, 5599, 5830, 7449
MabI ACCWGGT 1 cut(s) 7018
MaeII ACGT 6 cut(s) 226, 1312, 1504, 7144, 7528, 7608
MfeI CAATTG 3 cut(s) 2079, 3674, 7540
MflI RGATCY 8 cut(s) 259, 1926, 2992, 3160, 4093, 5468, 5537, 7661
MlsI TGGCCA 2 cut(s) 2495, 7589
MluNI TGGCCA 2 cut(s) 2495, 7589
MmeI TCCRAC 9 cut(s) 48, 1019, 2778, 3138, 3208, 4227, 5610, 5962, 7398
Mox20I TGGCCA 2 cut(s) 2495, 7589
Mph1103I ATGCAT 1 cut(s) 1881
MroI TCCGGA 1 cut(s) 4460
MroXI GAANNNNTTC 2 cut(s) 3466, 4305
MscI TGGCCA 2 cut(s) 2495, 7589
MslI CAYNNNNRTG 4 cut(s) 2810, 5955, 5976, 6603
Msp20I TGGCCA 2 cut(s) 2495, 7589
MspA1I CMGCKG 2 cut(s) 3382, 7411
MspCI CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
MspI CCGG 9 cut(s) 1028, 2679, 3907, 4164, 4461, 5283, 5432, 7594, 7740
MunI CAATTG 3 cut(s) 2079, 3674, 7540
Mva1269I GAATGC 4 cut(s) 1881, 4634, 5121, 5962
MvnI CGCG 1 cut(s) 7487
NciI CCSGG 3 cut(s) 3908, 5284, 7741
NdeI CATATG 3 cut(s) 607, 4128, 5785
NmeAIII GCCGAG 3 cut(s) 2347, 3369, 6613
NmuCI GTSAC 9 cut(s) 54, 340, 672, 1030, 1841, 3290, 5372, 5927, 7733
NsbI TGCGCA 2 cut(s) 2776, 5506
NsiI ATGCAT 1 cut(s) 1881
NspI RCATGY 6 cut(s) 535, 1736, 3431, 3456, 4337, 4607
NspV TTCGAA 1 cut(s) 6691
OliI CACNNNNGTG 3 cut(s) 2810, 5976, 6603
PaeI GCATGC 3 cut(s) 1736, 4337, 4607
PaeR7I CTCGAG 2 cut(s) 1439, 5495
PagI TCATGA 3 cut(s) 3090, 5011, 6580
PaqCI CACCTGC 2 cut(s) 3848, 6501
PceI AGGCCT 1 cut(s) 4414
PciI ACATGT 2 cut(s) 531, 3427
PciSI GCTCTTC 2 cut(s) 1398, 4866
PctI GAATGC 4 cut(s) 1881, 4634, 5121, 5962
PdmI GAANNNNTTC 2 cut(s) 3466, 4305
Pfl23II CGTACG 1 cut(s) 4328
PflFI GACNNNGTC 1 cut(s) 13
PflMI CCANNNNNTGG 3 cut(s) 906, 1930, 3190
PfoI TCCNGGA 2 cut(s) 1929, 4647
PinAI ACCGGT 1 cut(s) 7593
Ppu21I YACGTR 2 cut(s) 1313, 1505
PscI ACATGT 2 cut(s) 531, 3427
PshBI ATTAAT 2 cut(s) 378, 2010
PsiI TTATAA 1 cut(s) 6981
Psp124BI GAGCTC 2 cut(s) 1835, 4878
PspFI CCCAGC 2 cut(s) 6476, 7384
PspLI CGTACG 1 cut(s) 4328
PspPI GGNCC 8 cut(s) 749, 1025, 1982, 1994, 3759, 4555, 5802, 7536
PspXI VCTCGAGB 1 cut(s) 1439
PsrI GAACNNNNNNTAC 2 cut(s) 1853, 1885
PstI CTGCAG 2 cut(s) 2100, 5665
PstNI CAGNNNCTG 3 cut(s) 854, 1930, 7568
PsuI RGATCY 8 cut(s) 259, 1926, 2992, 3160, 4093, 5468, 5537, 7661
PsyI GACNNNGTC 1 cut(s) 13
PvuII CAGCTG 2 cut(s) 3382, 7411
RseI CAYNNNNRTG 4 cut(s) 2810, 5955, 5976, 6603
SacI GAGCTC 2 cut(s) 1835, 4878
SapI GCTCTTC 2 cut(s) 1398, 4866
Sau96I GGNCC 8 cut(s) 749, 1025, 1982, 1994, 3759, 4555, 5802, 7536
ScaI AGTACT 1 cut(s) 4449
SexAI ACCWGGT 1 cut(s) 7018
SfcI CTRYAG 3 cut(s) 2096, 5181, 5661
Sfr274I CTCGAG 2 cut(s) 1439, 5495
SfuI TTCGAA 1 cut(s) 6691
SinI GGWCC 4 cut(s) 749, 1994, 4555, 7536
SlaI CTCGAG 2 cut(s) 1439, 5495
SmiMI CAYNNNNRTG 4 cut(s) 2810, 5955, 5976, 6603
SnaBI TACGTA 1 cut(s) 1505
SphI GCATGC 3 cut(s) 1736, 4337, 4607
SseBI AGGCCT 1 cut(s) 4414
SsiI CCGC 9 cut(s) 464, 780, 4979, 5135, 5199, 6036, 6396, 7232, 7487
SspI AATATT 3 cut(s) 1599, 6012, 6341
SstI GAGCTC 2 cut(s) 1835, 4878
StuI AGGCCT 1 cut(s) 4414
StyI CCWWGG 2 cut(s) 2496, 7123
TaiI ACGT 6 cut(s) 229, 1315, 1507, 7147, 7531, 7611
TaqII GACCGA 2 cut(s) 1196, 1982
TatI WGTACW 8 cut(s) 84, 2844, 4447, 4654, 4662, 4716, 4918, 6828
TauI GCSGC 4 cut(s) 4982, 6039, 6398, 7490
TseFI GTSAC 9 cut(s) 54, 340, 672, 1030, 1841, 3290, 5372, 5927, 7733
Tsp45I GTSAC 9 cut(s) 54, 340, 672, 1030, 1841, 3290, 5372, 5927, 7733
TspGWI ACGGA 7 cut(s) 2641, 3488, 3827, 4316, 4547, 5885, 7627
Tth111I GACNNNGTC 1 cut(s) 13
Van91I CCANNNNNTGG 3 cut(s) 906, 1930, 3190
Vha464I CTTAAG 4 cut(s) 2627, 5153, 6571, 7496
VneI GTGCAC 1 cut(s) 568
VpaK11BI GGWCC 4 cut(s) 749, 1994, 4555, 7536
VspI ATTAAT 2 cut(s) 378, 2010
XagI CCTNNNNNAGG 4 cut(s) 759, 3712, 4949, 5714
XbaI TCTAGA 2 cut(s) 2839, 7363
XceI RCATGY 6 cut(s) 535, 1736, 3431, 3456, 4337, 4607
XcmI CCANNNNNNNNNTGG 1 cut(s) 2717
XhoI CTCGAG 2 cut(s) 1439, 5495
XmiI GTMKAC 2 cut(s) 1241, 1449
XmnI GAANNNNTTC 2 cut(s) 3466, 4305
ZrmI AGTACT 1 cut(s) 4449
Zsp2I ATGCAT 1 cut(s) 1881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.