RchiOBHm_Chr2g0116921

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000223
Physical Location & Seq
Reverse (-)
29235366 .. 29245636
10271 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48993

Sequence Viewer

Length: 5967 bp
ATGATGGAAGGTTCACACAAGAAAAAGCCTAAAAAGCCCAACCTTTTGGCTGAGACTGTGTTTTCTTGGTCCCTTGATAACATTTTCAATGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCACTCTGTGGAGCATTACCTCGGGTGTTATCTGTATCCTTTATTGGAAGAAACAAGAGCGCAGGTCCATGCAAGTATGGAAACTATTTACAGAGCACCATTTGCTAAAGTAGTTGCTTTTGAAAAAGTGAAGCCATATGGAACAAAGCTATATGATATCAAGGTTGATTATTGGCGAAACAGGTTAAATGACCGTGGCAAAGAGCCATATGAAACTTTGCCAGGTGATCTTTTTGTTTTGGCAAATGCTAAACCTGAAAGTTTTTCAGATTTACAAAGGGTAGGGAGGTCATGGGCTTTTGCATCAGTCACTAAAGTCTCAGAAAATGAGAACGAGGATGAAAGTACTTCTCTTTATTTTAAAATCAAGGCTTCCAAAGAACTTGAAGTTGTAAAGGGTTCGGCATCACTGTTTATGGTTTTTCTAGTGAACTTAATCCCGAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAACATTATCAAGGAAGTTCTGTGCACTGATTCTGTGGCTCATGAAAATCTCTGCTCTGAAAAGAATAATGACATCGTGAATAAGGGGTTAGTTCAGAGTTTATCATCTGGTCTGAATGAATCCCAAACAGGGACTGTTTTAGCCTGCCTTGAAATGCTGCATTGTCGTGAGAAGTCTGCTGTTGAACTAATTTGGGGTCCTCCTGGTACTGGAAAAACTAAAACAATTGCGACTCTACTTGTAACCTTGTTACAGATGAATTGTAGGACTCTTGTTTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGTGTTGTAAAGATGTTGACTGAAGTACAGTCCAGTTCTTTGTTTTGTTCTTTCGGAGAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAGAGTTGGCTCAGATATTGAAGAGATATATCTAGATTACCGTCTCAAAAGGCTTGCCGAGTGCTTAGGACCACAGACTGGCTGGAGGCATTGCTTTGCTTCCATGATAGATTTTCTTGAAGATTGTGTTTCTCACTACCATAACTTTTTGGTAAATGAGTTAACCATAGAGAAGGAACCAAAGACCTTAGGTCAAATGAAAGGAAAAGAAAGCAAGTGTGACACTCAAGTTGGCGAGGAGAAGTGCAAATCATTTCTGGAATTTATTAGAGACAGATTTGTTTCTAATGCATCACCTCTAAGAAATTGTATTGCTATCTTATGTACCCACATGGCGAAAAATTACATTTGTGGAGATAATGTCCAAAACATGATTATGCTTATCAACTTGGTTGATTCCTTTGAATCATTGTTGTTTCAAGATAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCTTGTTCTGACATTGACGACATATGGGAGACATATGCTGATAACTCATTTCATCTATTCATGAATAGAAGAGAATGCCTTAAAGTCTTGCACACTCTGTTTGACTCCCTTAGTGAACTTCACATTCCGAATTTTGTGAAGCAAGAGTCTCTAATGGAATTTTGTTTTCAAAATGCCTCCTTGATATTTTGCACTGCATCCAGCTCATATAAGTTGCATAAAGTGGCAATGGAGCCGTTGAACGTTGTTGTTATTGATGAAGCTGCACAATTGAAAGAGTCTGAATCAACAATCCCCCTTCAACTATGGGGTGTGAAGCATGCTGTTCTAGTTGGGGATGAATGTCAGTTACCAGCTACAGTGAAGAGCATTGTATCTGATGAAGCAGGTTTTGCAAGAAGTTTGTTCGAGAGGTTAAGCTCATTTGGTCGCTCGAAACACCTTCTAAATATGCAATACAGGATGCATCCATCAATAAGTTTCTTCCCAAATTCTAGATTCTACAATAACATGATCCTGGATGCTCCAAATCTTAAAAGTAGAAGCCAGAAGAAACACTATCTTAAAGGGTCAATGTTTGGTCCTTATTCTTTCATCAACGTAATTGGTGGACGAGAAGAGAAGGATGAGGATGGACGTAGTCGAAAGAATATGGTGGAGGTTGCTATTGTTTCACAAATACTGCGGAATCTCTACAAAAAATGGGTTGAATCAAAGCAGAAACTCAGTATTGGTATAGTATCTCCATATGCTGCTCAAGTAGTCGCAATCGAGGACAAACTTGCACAGAAGTATAACAATCTTGATGGCTTTAAAGTGAAGGTAAAGACAGTTGACGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTGTCCACTGTACGATCCAATTATCAGCGGTCACTTGAGTTTGTTTCAAAACCGCAAAGAATTAATGTTGCTCTTACAAGGGCTAGGCACTGTCTATGGATTTTGGGGAATGAAAAAATTCTATGTCATAATGAGTCTGTTTGGAAGGCCGTGGTCCTTGATGCCAAAAATCGCCATTGCTTTTTTAATGTGGATGAAGACAAGGATCTAGCCAAGGCCATATTGGAGGTTAAGAAACAGTACGACCAACTAGATGATTTGCTTGATGCTGACAGTATACTTTTCAGAAGTGCTAGATGGAAGGTGCTTTTTAGTGATAACTTTTTTAAGTCATTCAAGAAACTGACATCAGTCCGTTTGAAGAGGTCGGTACTAAATGTTCTACTGAAACTTGCCAGTGGCTGGAGACCAAAGAAGAAGAGTTCAGAAATGATTTGTGGTAGCTCTTCACTGATCTTGAAGAAATTTAAGGTTGAAAATCTGTATGTTGTTTGTACAACAGACATTGCAAAGGACTCAAGATACATTCAAGTATTGAAGATTTGGGACATATTGCCCATAGGGGATGTCCCAACTTTGGTCAATCGCCTGGACCGTATAATGAACAAATATACTGATGCTTTCATTAATCTCTGTAAGGAGAAATGTCTTGAGGGTGATTTGGAAATTCCAAAGAGCTGGCCACCTTCTTTGGAATTTTTGCGGTTTAAGGATTTTAGCTTCACTAAAATCCAGAATGATTTTGTTGGTGACACATGTGATGGTAGAAGTTACGCTGAGAATTCACGGGTTAGCGAAAGTTTGTTGCTGATGAAGTTCTACTCATTATCATCTGGGGTAGTAAACCACTTGCTGTCTGATTGTGAGGGTAGAGAACTGGATCTTCCATTTGAAGTTACGGACCAAGAAATGGAGATTATCCTTCATCATAGAAGTTCCTTCATAGTTGGAAGGTCAGGAACTGGGAAAACCACTGTTTTGACAATGAAATTATTTCAGAAAGAACAGTGGCACCAGTTGGCAGTGCAAGGATACTATACTAGTCAAAGCAGCAAGATTGAAAAGAGTTCAGCAGCTACTGAGGAGAAAGTTCTACACCAGCTTTTCGTTACAGTGAGTCCTAAGCTGTGTTATGCCATCAAGCAACATGTCTTACACTTGAAAAGTTTTGCATGTGGTGGAAGTCATTCGACTGAAAGCAGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTCAACGATATCCAAGATTCATTTCATGAAATTCCTCCCCATTCTTACCCTCTTTTCATCACATTTCATAAGTTCTTGATTATGCTTGATGGAACACTGAGCAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGCTTACTCATGGTCATCTACGGAATTCAAGATCAGCTGCCTTGCAGACTTTTATAAGAACAAAGGAAGTCAGTTATGAGAGGTTTAGTTCATTATACTGGCCGCATTTTAATACCGATTTAACAAAGAGGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCCCATATAAAAGGTGGCTTGGGAGCTATAGAAGCATGTGATGGTAAACTCAGTCGAGAGGATTATGTACAACTGTCAGAGATCCGAGTTTCCATTCTAACCAGGCAAGAGAGAGAAGTTATATATGATATTTTTCAGATGTATGAAAAAATGAAGATGGAAAATGGGGAATTTGATCTTACTGATTTTGTAATTGATCTTCACCATCGACTGATGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATGTTGATGAGGTTCAAGATCTAACAATGAGTCAAATTGCCTTGTTTAAACATATGTGCCACAATGTTGAAGATGGTTTTGTTTTTTCCGGTGACACTGCACAAACAATTGCAAGGGGTATTGATTTCAGGTTTCAAGAATTACGACATCTGTTCTACAAGAAGTTTGTGTTGGAGTCAAGAACCAATAAGCACGATGAAAGTAAGGAAAAAGGCCAAGTCTCGGAAATTCTTCACTTGACTCAAAACTTTCGTACCCATGATGGAATCCTCAAGCTATCACAAAGCATTATTGACCTACTATATCATTTCTTTCCCCTTTCCATTGATGTCTTAAAACCTGAAAGTAGTTTGATATTTGGGGAAGCTCCGGTATTGCTTGAATCTGAAGAAAATGAAAATGCAATCGTAAAGATTTTTGGAAATACTGGGAATATAGTTGGATTTGGTGCAGAGCAGGTAATTTTGGTCCGAGATGATAGTGCTCGAAAAGAGATATCCAAATTTATAGGGAAGCATGCTCTTGTTCTAACAATTGTGGAGTGCAAGGGCCTTGAGTTTCAGGATGTCCTCTTATACAACTTTTTTGGCTCATCACCTTCAAAAAACCAGTGGAGGGTTATATATGATTACATGAATGAGCTAGATTTACTCGACCACAGTTTACCCAGGGGCTTTCCTGGTTTCGATGTAGCCAAACACAATATCTTATGCTCTGAACTGAAGCAACTATATGTGGCCGTTACTCGCACGAGGCAGAGATTGTGGATTTGTGAAAATATGGAAGAGCTGTCCAAACCAATGTTTGACTACTGGAAGAAGAAATGTCTTGTGCAAGTTAAGCAACTGGATGATTCCCTTGCACATGAAATGCAAGTTGCAAGCAGTCCTGAGGAGTGGAAGTCACGTGGCATTAAGCTATATCATGAACACAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCAGGTGACACTTACTGGGAAAGAAGGTCTAAAGCAGCTGGCCTTAAAGCACTTGCTGCCCATATACGAACGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTAACGCTATAGGCAAGGCAGATTCTGCTGCCAGATGTTTTTCTGATCTGGGGGAGTATGAAAGAGCTGCTAGAATATATTTGAAATGTGGGGAGTCTGAACTGAAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGACGATATAAGGAGGCAGCAGATGTCTATGCTAAGGGTAATTTTTTCGCAGAGTGTCTCACTATTTGTGAAAAGGGAAAACTATTTGAACTGGGTTTGCAGTACATCAATAACTGGAAACAGCATGCAGAAGAGGACACTGCTATGACTACTAGAGGAAAAGGAATAAATAGAATGCAGCTTGAATTTTTAGAGAGCTGTGCACTTCACTATTATGATTTGAATGATAACAGATCCATGATTAAGTTTATTAAAGCTTTTCCTTCTATAACTTTGGTGCGGGACTTTTTGAAGAGGTTGGATAGCCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCAAACATCGCACAGCTTAAAGGTGAAATTCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAAGCCTCATTGAAAATAATATTCTACGTACTTGCCATGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCTGAAGAAGTTTCCACAACAGGAGGCCCTCTTGGCAAAAGCGCCAAGAATGAGACAGACAACTTTCATGAGTTGGTTTGCACTGAGGTTGATATTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

1988

Amino Acids

227.96

Weight (kDa)

6.45

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 78 - 206 6.5e-64 Domain of unknown function (DUF6469)
AAA_11 PF13086 244 - 616 2.3e-35 AAA domain
AAA_19 PF13245 264 - 612 3.3e-07 AAA domain
AAA_12 PF13087 625 - 821 1.5e-57 AAA domain
UvrD-helicase PF00580 1092 - 1418 1.5e-06 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3782
AarI CACCTGC 1 cut(s) 5102
Acc36I ACCTGC 5 cut(s) 187, 1856, 4591, 5102, 5771
AccB1I GGYRCC 1 cut(s) 3360
AccB7I CCANNNNNTGG 3 cut(s) 1100, 2751, 3259
AccI GTMKAC 1 cut(s) 2626
AccII CGCG 1 cut(s) 5345
AciI CCGC 8 cut(s) 2164, 2377, 2402, 3052, 3830, 5343, 5345, 5644
AclI AACGTT 1 cut(s) 1722
AclWI GGATC 7 cut(s) 1987, 2358, 2562, 3237, 3967, 4134, 5592
AcoI YGGCCR 4 cut(s) 3029, 3827, 4881, 5088
AcuI CTGAAG 8 cut(s) 942, 975, 1479, 4551, 4886, 5211, 5354, 5888
AcvI CACGTG 1 cut(s) 5051
AdeI CACNNNGTG 2 cut(s) 142, 5097
AflIII ACRYGT 3 cut(s) 603, 3104, 3496
AhdI GACNNNNNGTC 1 cut(s) 1212
AhlI ACTAGT 1 cut(s) 3389
AjnI CCWGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
AjuI GAANNNNNNNTTGG 4 cut(s) 3597, 3629, 4298, 4330
AloI GAACNNNNNNTCC 2 cut(s) 5170, 5202
Alw21I GWGCWC 4 cut(s) 232, 644, 4630, 5569
Alw44I GTGCAC 2 cut(s) 640, 5565
AlwI GGATC 7 cut(s) 1987, 2358, 2562, 3237, 3967, 4134, 5592
AlwNI CAGNNNCTG 5 cut(s) 752, 2751, 3311, 3428, 5255
Ama87I CYCGRG 1 cut(s) 155
ApaLI GTGCAC 2 cut(s) 640, 5565
AseI ATTAAT 2 cut(s) 2412, 2976
Asp700I GAANNNNTTC 6 cut(s) 120, 132, 996, 2466, 3535, 4374
AspLEI GCGC 2 cut(s) 196, 5908
AsuHPI GGTGA 9 cut(s) 371, 1308, 3017, 3110, 4085, 4247, 4731, 5126, 5756
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 9 cut(s) 69, 199, 815, 1091, 2060, 2503, 2941, 3250, 4612
AxyI CCTNAGG 2 cut(s) 1210, 5034
BaeGI GKGCMC 3 cut(s) 644, 901, 5569
BaeI ACNNNNGTAYC 2 cut(s) 4381, 4414
BalI TGGCCA 2 cut(s) 3031, 5090
BanI GGYRCC 1 cut(s) 3360
BarI GAAGNNNNNNTAC 2 cut(s) 4074, 4106
BauI CACGAG 2 cut(s) 933, 4894
BbrPI CACGTG 1 cut(s) 5051
BbsI GAAGAC 2 cut(s) 1476, 2553
Bbv12I GWGCWC 4 cut(s) 232, 644, 4630, 5569
BceAI ACGGC 3 cut(s) 1699, 2483, 4868
BciT130I CCWGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
BciVI GTATCC 2 cut(s) 180, 3374
BcuI ACTAGT 1 cut(s) 3389
BfmI CTRYAG 3 cut(s) 1836, 3918, 5238
BfoI RGCGCY 1 cut(s) 5909
BfuAI ACCTGC 5 cut(s) 187, 1856, 4591, 5102, 5771
BfuI GTATCC 2 cut(s) 180, 3374
BglI GCCNNNNNGGC 1 cut(s) 5897
BglII AGATCT 1 cut(s) 4162
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
Bme18I GGWCC 9 cut(s) 69, 199, 815, 1091, 2060, 2503, 2941, 3250, 4612
BmeRI GACNNNNNGTC 1 cut(s) 1212
BmeT110I CYCGRG 1 cut(s) 155
BmiI GGNNCC 6 cut(s) 71, 816, 1200, 1713, 2321, 3362
BmrFI CCNGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
BmrI ACTGGG 4 cut(s) 3321, 4581, 5134, 5465
BmuI ACTGGG 4 cut(s) 3321, 4581, 5134, 5465
BoxI GACNNNNGTC 1 cut(s) 2699
BpiI GAAGAC 2 cut(s) 1476, 2553
BpmI CTGGAG 2 cut(s) 1126, 2773
Bpu10I CCTNAGC 3 cut(s) 1087, 3471, 5397
BpuEI CTTGAG 7 cut(s) 1233, 2220, 2405, 2851, 3020, 4400, 4718
BsaAI YACGTR 2 cut(s) 5051, 5821
BsaI GGTCTC 1 cut(s) 2749
BsaJI CCNNGG 9 cut(s) 154, 328, 2323, 2499, 2562, 4811, 4812, 5343, 5770
BsaWI WCCGGW 2 cut(s) 4232, 4513
BsaXI ACNNNNNCTCC 4 cut(s) 1368, 1398, 5030, 5060
Bse21I CCTNAGG 2 cut(s) 1210, 5034
Bse3DI GCAATG 4 cut(s) 1111, 1713, 2524, 2853
BseBI CCWGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
BseDI CCNNGG 9 cut(s) 154, 328, 2323, 2499, 2562, 4811, 4812, 5343, 5770
BseMI GCAATG 4 cut(s) 1111, 1713, 2524, 2853
BseRI GAGGAG 3 cut(s) 1274, 3446, 5051
BseSI GKGCMC 3 cut(s) 644, 901, 5569
BsgI GTGCAG 3 cut(s) 1728, 4227, 4614
Bsh1236I CGCG 1 cut(s) 5345
BshNI GGYRCC 1 cut(s) 3360
BsiHKAI GWGCWC 4 cut(s) 232, 644, 4630, 5569
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 2 cut(s) 4233, 4514
BslFI GGGAC 5 cut(s) 55, 763, 2903, 2909, 5660
BsmBI CGTCTC 2 cut(s) 101, 1070
BsmFI GGGAC 5 cut(s) 55, 763, 2903, 2909, 5660
BsmI GAATGC 2 cut(s) 1559, 5544
Bso31I GGTCTC 1 cut(s) 2749
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 5 cut(s) 232, 644, 901, 4630, 5569
Bsp1407I TGTACA 2 cut(s) 2843, 3958
BspACI CCGC 8 cut(s) 2164, 2377, 2402, 3052, 3830, 5343, 5345, 5644
BspFNI CGCG 1 cut(s) 5345
BspHI TCATGA 5 cut(s) 658, 1539, 3616, 5068, 5931
BspLI GGNNCC 6 cut(s) 71, 816, 1200, 1713, 2321, 3362
BspMI ACCTGC 5 cut(s) 187, 1856, 4591, 5102, 5771
BspPI GGATC 7 cut(s) 1987, 2358, 2562, 3237, 3967, 4134, 5592
BspQI GCTCTTC 3 cut(s) 1838, 2800, 4923
BspT107I GGYRCC 1 cut(s) 3360
BspTNI GGTCTC 1 cut(s) 2749
BsrDI GCAATG 4 cut(s) 1111, 1713, 2524, 2853
BsrGI TGTACA 2 cut(s) 2843, 3958
BssECI CCNNGG 9 cut(s) 154, 328, 2323, 2499, 2562, 4811, 4812, 5343, 5770
BssNAI GTATAC 1 cut(s) 2627
BssSI CACGAG 2 cut(s) 933, 4894
BssT1I CCWWGG 2 cut(s) 2562, 5770
Bst1107I GTATAC 1 cut(s) 2627
Bst2BI CACGAG 2 cut(s) 933, 4894
Bst2UI CCWGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
BstAPI GCANNNNNTGC 4 cut(s) 236, 1871, 5165, 5255
BstAUI TGTACA 2 cut(s) 2843, 3958
BstBAI YACGTR 2 cut(s) 5051, 5821
BstC8I GCNNGC 9 cut(s) 763, 1077, 1800, 3029, 4662, 5026, 5149, 5490, 5861
BstDSI CCRYGG 3 cut(s) 328, 2499, 5343
BstENI CCTNNNNNAGG 1 cut(s) 5774
BstFNI CGCG 1 cut(s) 5345
BstH2I RGCGCY 1 cut(s) 5909
BstHHI GCGC 2 cut(s) 196, 5908
BstNI CCWGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
BstNSI RCATGY 8 cut(s) 607, 1802, 3108, 3500, 3525, 3930, 4664, 5492
BstPAI GACNNNNGTC 1 cut(s) 2699
BstSCI CCNGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
BstSFI CTRYAG 3 cut(s) 1836, 3918, 5238
BstSLI GKGCMC 3 cut(s) 644, 901, 5569
BstSNI TACGTA 1 cut(s) 5821
BstUI CGCG 1 cut(s) 5345
BstV2I GAAGAC 2 cut(s) 1476, 2553
BstX2I RGATCY 5 cut(s) 2554, 3229, 3972, 4162, 5597
BstXI CCANNNNNNTGG 2 cut(s) 46, 3027
BstYI RGATCY 5 cut(s) 2554, 3229, 3972, 4162, 5597
BstZ17I GTATAC 1 cut(s) 2627
Bsu36I CCTNAGG 2 cut(s) 1210, 5034
BsuI GTATCC 2 cut(s) 180, 3374
BtgI CCRYGG 3 cut(s) 328, 2499, 5343
BtgZI GCGATG 1 cut(s) 5713
BtsI GCAGTG 4 cut(s) 1671, 3378, 4239, 5502
BveI ACCTGC 5 cut(s) 187, 1856, 4591, 5102, 5771
Cac8I GCNNGC 9 cut(s) 763, 1077, 1800, 3029, 4662, 5026, 5149, 5490, 5861
CaiI CAGNNNCTG 5 cut(s) 752, 2751, 3311, 3428, 5255
CciI TCATGA 5 cut(s) 658, 1539, 3616, 5068, 5931
CfoI GCGC 2 cut(s) 196, 5908
Cfr42I CCGCGG 1 cut(s) 5346
CspCI CAANNNNNGTGG 2 cut(s) 3021, 3056
DraI TTTAAA 3 cut(s) 496, 2293, 4192
DraIII CACNNNGTG 2 cut(s) 142, 5097
DriI GACNNNNNGTC 1 cut(s) 1212
EaeI YGGCCR 4 cut(s) 3029, 3827, 4881, 5088
Eam1105I GACNNNNNGTC 1 cut(s) 1212
Eco105I TACGTA 1 cut(s) 5821
Eco130I CCWWGG 2 cut(s) 2562, 5770
Eco31I GGTCTC 1 cut(s) 2749
Eco32I GATATC 3 cut(s) 292, 3601, 4641
Eco47I GGWCC 9 cut(s) 69, 199, 815, 1091, 2060, 2503, 2941, 3250, 4612
Eco57I CTGAAG 8 cut(s) 942, 975, 1479, 4551, 4886, 5211, 5354, 5888
Eco72I CACGTG 1 cut(s) 5051
Eco81I CCTNAGG 2 cut(s) 1210, 5034
Eco88I CYCGRG 1 cut(s) 155
EcoNI CCTNNNNNAGG 1 cut(s) 5774
EcoO109I RGGNCCY 3 cut(s) 815, 4693, 5890
EcoRI GAATTC 2 cut(s) 3130, 3751
EcoRII CCWGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
EcoRV GATATC 3 cut(s) 292, 3601, 4641
EcoT14I CCWWGG 2 cut(s) 2562, 5770
EcoT22I ATGCAT 3 cut(s) 1315, 1947, 4110
ErhI CCWWGG 2 cut(s) 2562, 5770
Esp3I CGTCTC 2 cut(s) 101, 1070
FaqI GGGAC 5 cut(s) 55, 763, 2903, 2909, 5660
FauI CCCGC 1 cut(s) 5637
FauNDI CATATG 7 cut(s) 271, 343, 1502, 1513, 2227, 4131, 4197
FblI GTMKAC 1 cut(s) 2626
GlaI GCGC 2 cut(s) 195, 5907
GsuI CTGGAG 2 cut(s) 1126, 2773
HaeII RGCGCY 1 cut(s) 5909
HapII CCGG 2 cut(s) 4233, 4514
HhaI GCGC 2 cut(s) 196, 5908
Hin6I GCGC 2 cut(s) 194, 5906
HinP1I GCGC 2 cut(s) 194, 5906
HincII GTYRAC 3 cut(s) 951, 1185, 2314
HindII GTYRAC 3 cut(s) 951, 1185, 2314
HindIII AAGCTT 2 cut(s) 594, 5619
HpaI GTTAAC 1 cut(s) 1185
HpaII CCGG 2 cut(s) 4233, 4514
HphI GGTGA 9 cut(s) 371, 1308, 3017, 3110, 4085, 4247, 4731, 5126, 5756
HpyCH4IV ACGT 7 cut(s) 94, 1722, 2079, 2116, 5050, 5180, 5820
HpySE526I ACGT 7 cut(s) 94, 1722, 2079, 2116, 5050, 5180, 5820
HspAI GCGC 2 cut(s) 194, 5906
KspAI GTTAAC 1 cut(s) 1185
KspI CCGCGG 1 cut(s) 5346
LguI GCTCTTC 3 cut(s) 1838, 2800, 4923
LmnI GCTCC 6 cut(s) 145, 1015, 1711, 2008, 3914, 4516
MaeII ACGT 7 cut(s) 94, 1722, 2079, 2116, 5050, 5180, 5820
MfeI CAATTG 4 cut(s) 843, 1748, 4251, 4677
MflI RGATCY 5 cut(s) 2554, 3229, 3972, 4162, 5597
MhlI GDGCHC 5 cut(s) 232, 644, 901, 4630, 5569
MlsI TGGCCA 2 cut(s) 3031, 5090
MluNI TGGCCA 2 cut(s) 3031, 5090
MmeI TCCRAC 5 cut(s) 3277, 4296, 4564, 5643, 5670
Mox20I TGGCCA 2 cut(s) 3031, 5090
Mph1103I ATGCAT 3 cut(s) 1315, 1947, 4110
MroXI GAANNNNTTC 6 cut(s) 120, 132, 996, 2466, 3535, 4374
MscI TGGCCA 2 cut(s) 3031, 5090
MslI CAYNNNNRTG 4 cut(s) 783, 1397, 5507, 5577
Msp20I TGGCCA 2 cut(s) 3031, 5090
MspA1I CMGCKG 4 cut(s) 2377, 3764, 5147, 5345
MspI CCGG 2 cut(s) 4233, 4514
MspR9I CCNGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
MssI GTTTAAAC 1 cut(s) 4192
MunI CAATTG 4 cut(s) 843, 1748, 4251, 4677
Mva1269I GAATGC 2 cut(s) 1559, 5544
MvaI CCWGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
MvnI CGCG 1 cut(s) 5345
NdeI CATATG 7 cut(s) 271, 343, 1502, 1513, 2227, 4131, 4197
NlaIV GGNNCC 6 cut(s) 71, 816, 1200, 1713, 2321, 3362
NmeAIII GCCGAG 1 cut(s) 1105
NmuCI GTSAC 7 cut(s) 442, 1241, 2379, 3098, 4235, 5046, 5114
NsiI ATGCAT 3 cut(s) 1315, 1947, 4110
NspI RCATGY 8 cut(s) 607, 1802, 3108, 3500, 3525, 3930, 4664, 5492
PaeI GCATGC 3 cut(s) 1802, 4664, 5492
PagI TCATGA 5 cut(s) 658, 1539, 3616, 5068, 5931
PaqCI CACCTGC 1 cut(s) 5102
PasI CCCWGGG 1 cut(s) 4812
PciI ACATGT 3 cut(s) 603, 3104, 3496
PciSI GCTCTTC 3 cut(s) 1838, 2800, 4923
PcsI WCGNNNNNNNCGW 1 cut(s) 2941
PctI GAATGC 2 cut(s) 1559, 5544
PdmI GAANNNNTTC 6 cut(s) 120, 132, 996, 2466, 3535, 4374
PflFI GACNNNGTC 1 cut(s) 2118
PflMI CCANNNNNTGG 3 cut(s) 1100, 2751, 3259
PfoI TCCNGGA 1 cut(s) 1995
PmaCI CACGTG 1 cut(s) 5051
PmeI GTTTAAAC 1 cut(s) 4192
PmlI CACGTG 1 cut(s) 5051
Ppu21I YACGTR 2 cut(s) 5051, 5821
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 3 cut(s) 603, 3104, 3496
PshAI GACNNNNGTC 1 cut(s) 2699
PshBI ATTAAT 2 cut(s) 2412, 2976
PsiI TTATAA 1 cut(s) 3782
Psp1406I AACGTT 1 cut(s) 1722
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
PspCI CACGTG 1 cut(s) 5051
PspGI CCWGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
PspN4I GGNNCC 6 cut(s) 71, 816, 1200, 1713, 2321, 3362
PspPPI RGGWCCY 1 cut(s) 815
PsrI GAACNNNNNNTAC 2 cut(s) 2712, 2744
PstNI CAGNNNCTG 5 cut(s) 752, 2751, 3311, 3428, 5255
PsuI RGATCY 5 cut(s) 2554, 3229, 3972, 4162, 5597
PsyI GACNNNGTC 1 cut(s) 2118
PvuII CAGCTG 2 cut(s) 3764, 5147
RseI CAYNNNNRTG 4 cut(s) 783, 1397, 5507, 5577
SacII CCGCGG 1 cut(s) 5346
SapI GCTCTTC 3 cut(s) 1838, 2800, 4923
ScaI AGTACT 1 cut(s) 481
ScrFI CCNGG 8 cut(s) 357, 822, 1997, 2324, 2939, 3994, 4813, 4824
SduI GDGCHC 5 cut(s) 232, 644, 901, 4630, 5569
SfcI CTRYAG 3 cut(s) 1836, 3918, 5238
Sfr303I CCGCGG 1 cut(s) 5346
SgrBI CCGCGG 1 cut(s) 5346
SinI GGWCC 9 cut(s) 69, 199, 815, 1091, 2060, 2503, 2941, 3250, 4612
SmiMI CAYNNNNRTG 4 cut(s) 783, 1397, 5507, 5577
SmlI CTYRAG 7 cut(s) 1248, 2235, 2384, 2866, 2999, 4415, 4697
SmoI CTYRAG 7 cut(s) 1248, 2235, 2384, 2866, 2999, 4415, 4697
SnaBI TACGTA 1 cut(s) 5821
SpeI ACTAGT 1 cut(s) 3389
SphI GCATGC 3 cut(s) 1802, 4664, 5492
SsiI CCGC 8 cut(s) 2164, 2377, 2402, 3052, 3830, 5343, 5345, 5644
SspI AATATT 2 cut(s) 5229, 5814
StyD4I CCNGG 8 cut(s) 355, 820, 1995, 2322, 2937, 3992, 4811, 4822
StyI CCWWGG 2 cut(s) 2562, 5770
TaiI ACGT 7 cut(s) 97, 1725, 2082, 2119, 5053, 5183, 5823
TatI WGTACW 5 cut(s) 479, 958, 2843, 3958, 5466
TauI GCSGC 2 cut(s) 3832, 5345
TseFI GTSAC 7 cut(s) 442, 1241, 2379, 3098, 4235, 5046, 5114
Tsp45I GTSAC 7 cut(s) 442, 1241, 2379, 3098, 4235, 5046, 5114
TspGWI ACGGA 3 cut(s) 2693, 3263, 3763
Tth111I GACNNNGTC 1 cut(s) 2118
Van91I CCANNNNNTGG 3 cut(s) 1100, 2751, 3259
VneI GTGCAC 2 cut(s) 640, 5565
VpaK11BI GGWCC 9 cut(s) 69, 199, 815, 1091, 2060, 2503, 2941, 3250, 4612
VspI ATTAAT 2 cut(s) 2412, 2976
XagI CCTNNNNNAGG 1 cut(s) 5774
XbaI TCTAGA 2 cut(s) 1054, 1973
XceI RCATGY 8 cut(s) 607, 1802, 3108, 3500, 3525, 3930, 4664, 5492
XcmI CCANNNNNNNNNTGG 3 cut(s) 1101, 2569, 3902
XmiI GTMKAC 1 cut(s) 2626
XmnI GAANNNNTTC 6 cut(s) 120, 132, 996, 2466, 3535, 4374
ZrmI AGTACT 1 cut(s) 481
Zsp2I ATGCAT 3 cut(s) 1315, 1947, 4110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.