Rmu_sc0000372.1_g000002

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000372.1
OG0000223
Physical Location & Seq
Reverse (-)
5053 .. 15281
10229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000372.1_g000002.1.cds

Sequence Viewer

Length: 7857 bp
atggaaactattgacagtgcaccatttgcggaagtagttgattttaaagagtccaagccatatggaacaaagcaatatacagtcaaggttgattgttggcgaaacaggttcagtgaacgtggcaatgaaccatacaaaactttgcctggggatctttttattttagcaaatgctaaacctgaaactgttgctcaattacaactggctgggaggtcatgggcttttgtatctgtcactcatcttgtgtctaacatctcacaaattcagaaaaaagatgagaatggggatgaggttgaggatgtggaagaggaaacttctctttgttttaaaatcaagtcttcaaaagagtttgaagtcgatgatggcacatcagtgtctgttgttttcttgttgaatttaatccccagtgaaagaatatacaaagcattgcacatgctgggaaatttaacaattatcaaagaagttctatgctctgattctgtggctcaggaaaatcctttctatgagaagaataatgacatcatggagaaggggttagatgagaatttattgtcaggacttaatgaggcccaaactggggcagttttggcttgtcttggaatgcggcattgtggtgcaaagtcttcggtggaacttatatggggtcctcctgggacaggaaaaactaaaacaatcgctactctgctttttactctgttaaagatgaattgtaggactcttatttgtgccccaacaaatgttgcaataattgaagttgcttctcgtcttctgaaaatggtgacagaagcagagtccaatgcaatgttttgttcagttggaaaaatccttttatttgggaataaggaacgacttaaactcaaagttggttcagatattgaagagatatatttgggtcaccgtattcaaaaaatcatcgagtgcataggaccacaaactggttggaggcattgctttgcttccatgataaatttacttgaagattgtgtttgtgattacaatattttcttgggcaatgagttgactgaactcactgaaggggaagaacaaaatagtgcttgtgaagtgaatgatgaatgcagaagtggaccaggagttagtgaggggaagtgcaaaacatttcttgaatatttcagaactagatttctctctaccgcatcgccactaagatattgcatttctatcttctgtacccatatgaccaaaatctgcattccagaacacatattcaaggatatggtttctcttatcctcttagttgattcctttgagatgatgttgtttgaaggtaatgttatttcagaagcattggaaggccttttttcttgttcagaggttgagggtgtatctgagttatatgtagatgactcaattcctcttgtcatgaagagaaaggaatgccttgtagctttacgcactctacagtattctcttagcaaacttcaacttccagattttaggaaaggaactgatctaatggatttatgcttccaaggtgcatccttggtattttgcactgcatctagttcatttaagttgcatagactggaaatggagccatttacctttgtggttattgatgaagctgcacagttgaaagagtgtgagtcaactatacccctgcaactaccaggagcaaaacatgtaattcttgttggtgatgaatgccaattgccagctatggtgaaaagcaatgtttctcaagaagctggttttgcaagaagcttgtttgagaggttaagcacaacgggtcacccaaaacaccttctaaatatgcagtacagaatgcatccgtcaataagtttcttcccaaattctagtttctataataaccggatcctggacgctccaaatgtcacaaaaaaaagccatgagaagcactatcttccagggccaatatatggtccttattcttttataaatgtaaatgatggaagagaggagaaggatgaggatggtcatagtcgaaagaatatggttgaggtcgctattgttttgaaagtactgcggaatctttacaaagaatggatgaattcaaaacagaaactcagtgttggtgtggtatctccctatgctgctcaagtagtggcaattcaggaaaatcttagagaaaagtatgacaatcttgatggctttaaagtgaaggtaaatacagttgacgggttccagggtggggaagacgacataattataatgtccactgtaagatccaacagccatcaatcacttcagtttatttcaaatattcaaagagtcaatgtttctctcacaagggctaggcactgtctctggattttgggcaatgaaaaaactctatgcagtactgattctgtttggaaggccttggttcttgatgccaaaagtcgtgactgcttctttaatgctgatcaagacaaggatttggccaaggccatagtagacataaagaaagagttggaccaattggatgatttgctcaatgccgacagcatacttttcaaaaatgccagatggaaggtgctttttagtgataactttttgaagtcattcaagaaactgacgtcagcctctttgaagaagtctgtactaaaccttctactgaagctttcaactggctggagacctaggaagcgcaatgtagagacaggttgtagtagctcttcactaatcttgaagaaatttaaggttaaatctctatatcttgtttgcacaacggacattgcaaaggatattgtttgcacaacagacattgcaaaggatatgagatacattcaaatattaaaggtctgggacatattgctcctggaggatatcccaaagttggaatatcgcctggagactattctaaacagatatacagatgattttattagtctttgcaaggagaaatattgtgacggtgatttagaagttccacagagctggccactttctctggaattttcccagttcaaggatcctagtgtcactgaaaccaagagagattttgttggtgacacttctgatggcaggagttatgttgagaattcacaggtcagtgagagtttgttgctaatgaaattttactctttatcatctggtttggtgaaccacttgctctctgactgtgggggtagagaattggatctcccgtttgaagttacagaccaagaaagggagattatacttcatcatagaagttcttttatagttggacggtcaggtactgggaagaccaccgtcttaaccatgaagttattccaaagagaacaatggcaccatttggctgtgcagggatgttatggtactgaaaatagcaatgatgagcaaagttctgcaaatactgaagggaaaattttacgccagcttttcattacagtgagtcctaagctatgttttgccatcaagcaacatgttacacatttgaaaagttttgcatgtggtggaagtgactcagctgaaagaagtttgattgatatagttgattttgaagaggaggaatcccaattcaaggatatccaagattcatttcatgatattcctccgagttcttaccctcttgtgataacattctataagttcctgatgatgcttgatggaacattgagtaactcatactttgcaaggttctgcgatgcatcactttctcatggccaatttagaaattctagatcagttgtgttccaggcctttataaggacaaaagaagtcaattatgagaggtttagttcatcatattggccccattttaatactcagctaactaagaaccttgatgttgcaagagtctttacagagattatttctcatataaaaggtggcttgggaaccatagaagcatgtgatgggaagctcagcttggaggattatgttaaattgtcagagggccgagcttctaatttaagcaagcaaaagagagaacagatatatgagatatttcaagattatgaaaaaatgaagatgcgaaatggtgaatttgatctagctgattttgtaaatgatcttcaacgtcgactcaagcatgagaaatatatgggtgatcagatggattttatttatattgatgaggttcaagatctcacaatgagtcaaattgctttgttcaaacatatgtgcaacaatgttgatgagggatttgttttttctggtgatacagcacaaacaattgccaggggtattgattttaggttccaagatatacgacatctgttctacaagaagtttgtgttggagtcaagaagcaataagcaagatgaaagaaaaggaaaaggccaaatttctgaaatatatcatttgactcaaaacttccgtacccattctggtatattgaagttatcgcagagcattattgaattactatatcgcttttttcctctttcaattgatgttttaaaaccagaaagcagtctgatttatggggaagctccggttgtgcttgaatctggagaaaatgaaaatgcaatcatcaaaatatttggaaatagtagaaatatagttggctttggtgcagagcaggtgatattggtgcgagatgatagtactcgaagagaaatttccaatgttgttgggaagcatgctcttgtccttaccatagttgagtgcaagggccttgagtttcaggatgtactcttgtacaactttttcggctcatctccttcgaaaaatcaatggagggttatttatgattacatgaaggaacaagatttactcgactctacattacctaagaaatttccaagtttcagtgaagcgaaacacaacatattatgctctgaattgaagcaattatatgttgctgttactcgcaccagacagagattgtggatttgtgaaactgtagaggaaatttcgaaacccatgtttgactattggaagaagaaatgtcttgtgcatgttaagcagcttgatgattcacttgcacaagccatgcaagttgcaagcagcacagaggagtggagatcccgtgggattaagctctatcgtgagaataactacgagatggccacaatgtgctttaaaagatctggtgacacttattgggaacaaaggtccaaagctgctggccttcaagctattgctgaccatatgcgaatgtcaaatcctgaagaggctaattctattcttaggaaagctgctgaaatatttgatgctatagggaaggctgattctgctgcaagatgtttttctgatttgggagagtatgaaagagcagccaggatttattttgagaaatgtggcgaattggaaagagccgctgaatgtttcactctagcaggatgctatgaggatgctgcagatgtgtatgctaaaggcaattttttcttagagtgtctctctgtctgtgccaaaggaaaattatttgatagaggtttggaatacatcaattattggaagaggcatgcagtagagaacaattctttaactagaggaggagaggggatacataatatggaacaaaaatttctagagggctgtgcacgccactatcataagaagaaagataacagatccatgatgaaatttgtaaaagccttccggtctatatcattgatacggaattttttgaagagtttaggtagccttgatgaacttcttttgctggaagatcaatttggaaactatttggaagctgcggagattgcacagattaagggagaaattctacttaaatccgattatcttggaaaggcaggtgaattcaaagatgcctcaatgcatatcctattctatgtacttgccagctctctttggtcatctggcagcaagggctggcccatgaagaagtttgcacaacaggaagctcttttgaaaaaaaccaagtcagttgcaaagaatgagacagacgacttttatgagttggtttgcactgaggttgatattttattgaatgaacaggataatttggctctgttgaaacatcaaatgaaggcttctcagagacatgcaagcattataggtgaagtgttatctgctcgaaaaatgttggattcccatttttctctgaggactgataagtatttgtgggaaaaagaattgatcagtgattgtgtgaagcattctgaagatatgatatcccaaaaccaggtttccatcgactcactagttcacttctggaatatttggaaggaaaagattgttctcgtgattgaatgtctaggatgccttgaaacccaagatgttaatgtaaacagaagttatcaaaagttttgtttaaactacttgggagtatggaggctatctaatcaaatgaatcctgtgtacgttttactcttccctgatgctgattgggtcagagatgtggacaaaagttatttccaaagtcatgggaagttggtctccattgatgttcgccagcttatttcagctgctcagaaatattggagttcagaactgcttgctgtaggcattatggttttagaaaagcttgatgagctttccaagttctctatcaagagttctgattcaacattcttccagagcagggctcttacatccatctatgatgttgcggtgtatcacttgaattgcaaatgtttgaagagaaggcctcaagatgcccaggcactgcagaaatttgtaactttctcaactgaaaggtttgttgcttgcgtgtttcctttagattggagggaatcatttagagagaatatgatttctcttagaagaactgatgcttcaaagaatgtgctgaatcaagttatagttgaatacatcagctcaaagaacgagctgtcttatggtcaaattggaaggatcgcaatgaccattctcgggtctggtaagctgaattttgaactatgtaagaagcttgtgaaaaagttggactccaatccaccatggaaggactttattgagaacctatgtgctagtactgaacctggaaataccagtcagaagcctagagaggtgtctgttatgtggctattatctgaggctttagttgaaacttatgatgcagactggagggttgttaaagactacatatcacctggttgtttcttgtatcttgtggagcgtcttctgatttgggcttctcattgtcgaggttatgttatcaccacaagttcttgtttcactgaatggctgatgtaccaggatgaagatactcatgtgcaaccatctatagaagatactcatgtgcaaccatctatagaaggcatccttcattttgttagtaatgtggttaccgaatgcctttgggacaaggcaaatctgattgaatggattagaaagtctaatgcagattggaagacctgttattcactactcgtgccaagattggtgtttgtactatgtttgctttgtgtgaactttggaatatccttgggtgtactgtctgatatgctgggaagcagctatatcactgaacaccttccatcggaattttacgacacccttaagaggcggaggaggaacaattttctgaatataaatgaggaagtgcttgcagcagctctcaagaagattcataatcctttggtaattgcgagttttgggtttgattgttcaaggttcgtatgcccagatgccatctttgttgacatgaaggccattcagtccaaggatgacctattcagaataatgtttcctaaaccaccagtcattcaagttgcaccaggtcgaattggatttgttgaagctggaggtaatactctttgtgatggtgtactttctgcaaatgatatcgatgcagggaagagctctttaaactccaacttggtgggagaaactgatgagcagcttgatgctgaagacggtcagagtagaggtgaggaaatctgcaaagccggtgtgtctacaccaatgtgtccacatcctgatgcagtctcggcttctacttctgcaacccaagcaaacaaaaagggcaaccgcaagaataatactaagaaaaataaggcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

2618

Amino Acids

297.74

Weight (kDa)

6.05

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000223)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26390 FvH4_1g26391 FvH4_2g00700 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_2g00900 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36180 FvH4_4g36210 FvH4_4g36221 FvH4_4g36250 FvH4_4g36250 FvH4_4g36250 FvH4_4g36280 FvH4_4g36280 FvH4_5g34990
malus_domestica MD09G1274800.v1.1 MD09G1274900.v1.1 MD09G1275500.v1.1 MD13G1010700.v1.1 MD16G1270700.v1.1
prunus_persica Prupe.1G087400_v2.0.a1 Prupe.1G134300_v2.0.a1 Prupe.1G215600_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G215900_v2.0.a1 Prupe.1G342300_v2.0.a1 Prupe.8G106200_v2.0.a1 Prupe.8G106200_v2.0.a1
pyrus_communis pycom09g18530 pycom09g18550 pycom09g18590 pycom13g00880 pycom16g24010
rosa_chinensis RchiOBHm_Chr2g0116911 RchiOBHm_Chr2g0116921 RchiOBHm_Chr3g0486161 RchiOBHm_Chr3g0486171 RchiOBHm_Chr4g0445931 RchiOBHm_Chr4g0445961 RchiOBHm_Chr4g0445971 RchiOBHm_Chr4g0445991 RchiOBHm_Chr4g0446001 RchiOBHm_Chr4g0446011 RchiOBHm_Chr4g0446031
rosa_laevigata RLG00000002343 RLG00000005696 RLG00000005698 RLG00000005699 RLG00000005701 RLG00000005703 RLG00000018313 RLG00000023096 RLG00000023099
rosa_multiflora Rmu_co8408865.1_g000001 Rmu_co8420123.1_g000001 Rmu_sc0000372.1_g000002 Rmu_sc0003001.1_g000002 Rmu_sc0003001.1_g000003 Rmu_sc0003001.1_g000006 Rmu_sc0003001.1_g000007 Rmu_sc0006315.1_g000012 Rmu_sc0007511.1_g000001 Rmu_sc0008543.1_g000001 Rmu_sc0009534.1_g000001 Rmu_sc0024967.1_g000001 Rmu_sc0036469.1_g000001 Rmu_ssc0000238.1_g000032
rosa_roxburghii Rroxscaffold_2G00126890 Rroxscaffold_5G00386350 Rroxscaffold_5G00386360 Rroxscaffold_5G00386370 Rroxscaffold_5G00386380 Rroxscaffold_5G00386400 Rroxscaffold_5G00386410 Rroxscaffold_5G00386430 Rroxscaffold_5G00386440 Rroxscaffold_6G00396020
rosa_rugosa Rorug03G0223800 Rorug03G0223800 Rorug03G0223800 Rorug04G0366100 Rorug04G0366200 Rorug04G0366300 Rorug04G0366400 Rorug04G0366500 Rorug04G0366600 Rorug04G0366600 Rorug04G0366700 Rorug04G0366700
rosa_samantha Rh1AG167300 Rh2AG265000 Rh2BG276500 Rh2DG272400 Rh2DG291200 Rh2DG291300 Rh2DG291400 Rh3AG273600 Rh3AG273700 Rh3BG308800 Rh3BG309000 Rh3CG307200 Rh3DG303500 Rh4AG427400 Rh4AG427700 Rh4AG427800 Rh4AG427900 Rh4AG428000 Rh4AG428300 Rh4AG428400 Rh4AG428500 Rh4AG428600 Rh4AG428700 Rh4BG426000 Rh4BG437600 Rh4BG437800 Rh4BG437900 Rh4BG438100 Rh4CG454300 Rh4CG454500 Rh4CG454600 Rh4CG454700 Rh4CG454800 Rh4CG454900 Rh4CG455200 Rh4CG455500 Rh4DG435700 Rh4DG435800 Rh4DG435900 Rh4DG436000 Rh4DG436100 Rh4DG436300 Rh4DG436600
rosa_wichuraiana Rw2G020880 Rw3G024250 Rw4G036590 Rw4G036600 Rw4G036620 Rw4G036630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1916, 2189, 3650
AarI CACCTGC 2 cut(s) 4459, 5636
AatII GACGTC 1 cut(s) 2540
Acc36I ACCTGC 2 cut(s) 4459, 5636
AccB1I GGYRCC 1 cut(s) 3231
AccB7I CCANNNNNTGG 2 cut(s) 935, 1898
AccI GTMKAC 3 cut(s) 2415, 3967, 7751
AciI CCGC 9 cut(s) 29, 604, 1152, 2005, 5208, 5588, 6464, 7360, 7825
AcoI YGGCCR 4 cut(s) 2400, 2900, 3607, 4956
AcuI CTGAAG 7 cut(s) 1053, 2210, 2597, 3321, 5079, 6036, 7725
AcyI GRCGYC 1 cut(s) 2537
AdeI CACNNNGTG 1 cut(s) 4965
AflII CTTAAG 1 cut(s) 7352
AflIII ACRYGT 2 cut(s) 1639, 3367
AhlI ACTAGT 1 cut(s) 6055
AjuI GAANNNNNNNTTGG 8 cut(s) 3468, 3500, 3797, 3829, 4166, 4198, 5550, 5582
AleI CACNNNNGTG 2 cut(s) 371, 7759
Alw21I GWGCWC 3 cut(s) 22, 5434, 7658
Alw26I GTCTC 9 cut(s) 2288, 2590, 2612, 2807, 5291, 5786, 5887, 6295, 7786
Alw44I GTGCAC 2 cut(s) 18, 5430
AlwNI CAGNNNCTG 3 cut(s) 377, 3182, 6520
Ama87I CYCGRG 1 cut(s) 6725
ApaLI GTGCAC 2 cut(s) 18, 5430
Asp700I GAANNNNTTC 3 cut(s) 2522, 3212, 7326
AspA2I CCTAGG 1 cut(s) 2600
AspLEI GCGC 1 cut(s) 2610
AsuII TTCGAA 2 cut(s) 4613, 4805
AvaI CYCGRG 1 cut(s) 6725
AvaII GGWCC 6 cut(s) 644, 926, 1085, 1901, 2434, 5004
AvrII CCTAGG 1 cut(s) 2600
BaeGI GKGCMC 3 cut(s) 22, 730, 5434
BaeI ACNNNNGTAYC 6 cut(s) 4249, 4282, 7050, 7077, 7083, 7110
BalI TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
BamHI GGATCC 2 cut(s) 1833, 2932
BanI GGYRCC 1 cut(s) 3231
BanII GRGCYC 2 cut(s) 6442, 7658
BarI GAAGNNNNNNTAC 6 cut(s) 2553, 2585, 2620, 2652, 3942, 3974
BauI CACGAG 2 cut(s) 6095, 7223
BbsI GAAGAC 8 cut(s) 330, 615, 758, 2181, 3194, 6965, 7211, 7713
Bbv12I GWGCWC 3 cut(s) 22, 5434, 7658
BcgI CGANNNNNNTGC 2 cut(s) 606, 640
BciVI GTATCC 1 cut(s) 5388
BclI TGATCA 3 cut(s) 2383, 3994, 5991
BcoDI GTCTC 9 cut(s) 2288, 2590, 2612, 2807, 5291, 5786, 5887, 6295, 7786
BcuI ACTAGT 1 cut(s) 6055
BfmI CTRYAG 8 cut(s) 1418, 4791, 5106, 5247, 6354, 6521, 7077, 7104
BfrI CTTAAG 1 cut(s) 7352
BfuAI ACCTGC 2 cut(s) 4459, 5636
BfuI GTATCC 1 cut(s) 5388
BglII AGATCT 2 cut(s) 4030, 4976
BlnI CCTAGG 1 cut(s) 2600
BlpI GCTNAGC 1 cut(s) 3809
BmcAI AGTACT 4 cut(s) 2001, 2320, 4495, 6826
Bme18I GGWCC 6 cut(s) 644, 926, 1085, 1901, 2434, 5004
BmeT110I CYCGRG 1 cut(s) 6725
BmiI GGNNCC 9 cut(s) 645, 1554, 1835, 2162, 2934, 3233, 3698, 3784, 4145
BmrI ACTGGG 4 cut(s) 399, 585, 2917, 3192
BmuI ACTGGG 4 cut(s) 399, 585, 2917, 3192
BoxI GACNNNNGTC 1 cut(s) 3194
BpiI GAAGAC 8 cut(s) 330, 615, 758, 2181, 3194, 6965, 7211, 7713
BplI GAGNNNNNCTC 6 cut(s) 5273, 5305, 6424, 6456, 6487, 6519
BpmI CTGGAG 6 cut(s) 2614, 2801, 2831, 4419, 6937, 7617
Bpu10I CCTNAGC 2 cut(s) 486, 3342
Bpu1102I GCTNAGC 1 cut(s) 3809
Bpu14I TTCGAA 2 cut(s) 4613, 4805
BpuEI CTTGAG 6 cut(s) 1683, 2061, 3956, 4586, 6489, 7397
Bsa29I ATCGAT 1 cut(s) 7641
BsaHI GRCGYC 1 cut(s) 2537
BsaI GGTCTC 2 cut(s) 2590, 6295
BsaWI WCCGGW 3 cut(s) 1830, 4381, 5490
BsaXI ACNNNNNCTCC 4 cut(s) 518, 548, 4903, 4933
Bse118I RCCGGY 1 cut(s) 7742
BseCI ATCGAT 1 cut(s) 7641
BseRI GAGGAG 6 cut(s) 1952, 3464, 4919, 5397, 5400, 7378
BseSI GKGCMC 3 cut(s) 22, 730, 5434
BseYI CCCAGC 3 cut(s) 206, 436, 7300
BsgI GTGCAG 3 cut(s) 1569, 3266, 4482
BshNI GGYRCC 1 cut(s) 3231
BshVI ATCGAT 1 cut(s) 7641
BsiHKAI GWGCWC 3 cut(s) 22, 5434, 7658
BsiHKCI CYCGRG 1 cut(s) 6725
BsiSI CCGG 4 cut(s) 1831, 4382, 5491, 7743
BslFI GGGAC 3 cut(s) 667, 2780, 7169
BsmAI GTCTC 9 cut(s) 2288, 2590, 2612, 2807, 5291, 5786, 5887, 6295, 7786
BsmFI GGGAC 3 cut(s) 667, 2780, 7169
BsmI GAATGC 8 cut(s) 606, 1079, 1209, 1400, 1667, 1788, 6010, 7151
Bso31I GGTCTC 2 cut(s) 2590, 6295
BsoBI CYCGRG 1 cut(s) 6725
Bsp119I TTCGAA 2 cut(s) 4613, 4805
Bsp1286I GDGCHC 5 cut(s) 22, 730, 5434, 6442, 7658
Bsp1407I TGTACA 1 cut(s) 4587
Bsp1720I GCTNAGC 1 cut(s) 3809
Bsp19I CCATGG 1 cut(s) 6791
BspACI CCGC 9 cut(s) 29, 604, 1152, 2005, 5208, 5588, 6464, 7360, 7825
BspDI ATCGAT 1 cut(s) 7641
BspHI TCATGA 2 cut(s) 1380, 3487
BspLI GGNNCC 9 cut(s) 645, 1554, 1835, 2162, 2934, 3233, 3698, 3784, 4145
BspMAI CTGCAG 2 cut(s) 5251, 6525
BspMI ACCTGC 2 cut(s) 4459, 5636
BspQI GCTCTTC 2 cut(s) 2641, 7646
BspT104I TTCGAA 2 cut(s) 4613, 4805
BspT107I GGYRCC 1 cut(s) 3231
BspTI CTTAAG 1 cut(s) 7352
BspTNI GGTCTC 2 cut(s) 2590, 6295
BsrFI RCCGGY 1 cut(s) 7742
BsrGI TGTACA 1 cut(s) 4587
BssAI RCCGGY 1 cut(s) 7742
BssNI GRCGYC 1 cut(s) 2537
BssSI CACGAG 2 cut(s) 6095, 7223
BssT1I CCWWGG 8 cut(s) 1489, 1500, 2340, 2403, 2600, 6791, 7278, 7515
Bst2BI CACGAG 2 cut(s) 6095, 7223
BstACI GRCGYC 1 cut(s) 2537
BstAFI CTTAAG 1 cut(s) 7352
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 4587
BstBI TTCGAA 2 cut(s) 4613, 4805
BstDSI CCRYGG 2 cut(s) 4918, 6791
BstEII GGTNACC 3 cut(s) 893, 1748, 7138
BstENI CCTNNNNNAGG 2 cut(s) 654, 3649
BstHHI GCGC 1 cut(s) 2610
BstMAI GTCTC 9 cut(s) 2288, 2590, 2612, 2807, 5291, 5786, 5887, 6295, 7786
BstNSI RCATGY 9 cut(s) 436, 1643, 3371, 3396, 3798, 4532, 4850, 5357, 5900
BstPAI GACNNNNGTC 1 cut(s) 3194
BstPI GGTNACC 3 cut(s) 893, 1748, 7138
BstSFI CTRYAG 8 cut(s) 1418, 4791, 5106, 5247, 6354, 6521, 7077, 7104
BstSLI GKGCMC 3 cut(s) 22, 730, 5434
BstV2I GAAGAC 8 cut(s) 330, 615, 758, 2181, 3194, 6965, 7211, 7713
BstX2I RGATCY 9 cut(s) 151, 1833, 2204, 2932, 3100, 4030, 4913, 4976, 5462
BstXI CCANNNNNNTGG 3 cut(s) 2898, 6278, 7675
BstYI RGATCY 9 cut(s) 151, 1833, 2204, 2932, 3100, 4030, 4913, 4976, 5462
Bsu15I ATCGAT 1 cut(s) 7641
BsuI GTATCC 1 cut(s) 5388
BsuTUI ATCGAT 1 cut(s) 7641
BtgI CCRYGG 2 cut(s) 4918, 6791
BtgZI GCGATG 2 cut(s) 1140, 3603
BtsI GCAGTG 2 cut(s) 1512, 6518
BveI ACCTGC 2 cut(s) 4459, 5636
CaiI CAGNNNCTG 3 cut(s) 377, 3182, 6520
CciI TCATGA 2 cut(s) 1380, 3487
CfoI GCGC 1 cut(s) 2610
Cfr10I RCCGGY 1 cut(s) 7742
ClaI ATCGAT 1 cut(s) 7641
CseI GACGC 2 cut(s) 1850, 6959
CsiI ACCWGGT 3 cut(s) 6036, 6943, 7570
DraI TTTAAA 7 cut(s) 46, 328, 2134, 4347, 4972, 6168, 7662
DraIII CACNNNGTG 1 cut(s) 4965
EaeI YGGCCR 4 cut(s) 2400, 2900, 3607, 4956
EciI GGCGGA 1 cut(s) 7375
Ecl136II GAGCTC 1 cut(s) 7656
Eco130I CCWWGG 8 cut(s) 1489, 1500, 2340, 2403, 2600, 6791, 7278, 7515
Eco147I AGGCCT 4 cut(s) 1314, 2339, 3644, 6502
Eco24I GRGCYC 2 cut(s) 6442, 7658
Eco31I GGTCTC 2 cut(s) 2590, 6295
Eco32I GATATC 4 cut(s) 2788, 3472, 6027, 7639
Eco47I GGWCC 6 cut(s) 644, 926, 1085, 1901, 2434, 5004
Eco53kI GAGCTC 1 cut(s) 7656
Eco57I CTGAAG 7 cut(s) 1053, 2210, 2597, 3321, 5079, 6036, 7725
Eco88I CYCGRG 1 cut(s) 6725
Eco91I GGTNACC 3 cut(s) 893, 1748, 7138
EcoICRI GAGCTC 1 cut(s) 7656
EcoNI CCTNNNNNAGG 2 cut(s) 654, 3649
EcoO109I RGGNCCY 2 cut(s) 644, 4561
EcoO65I GGTNACC 3 cut(s) 893, 1748, 7138
EcoRI GAATTC 3 cut(s) 2029, 3001, 5651
EcoRV GATATC 4 cut(s) 2788, 3472, 6027, 7639
EcoT14I CCWWGG 8 cut(s) 1489, 1500, 2340, 2403, 2600, 6791, 7278, 7515
EcoT22I ATGCAT 3 cut(s) 1788, 3595, 5673
EcoT38I GRGCYC 2 cut(s) 6442, 7658
ErhI CCWWGG 8 cut(s) 1489, 1500, 2340, 2403, 2600, 6791, 7278, 7515
FalI AAGNNNNNCTT 4 cut(s) 3797, 3829, 7101, 7133
FaqI GGGAC 3 cut(s) 667, 2780, 7169
FauNDI CATATG 4 cut(s) 61, 1194, 4065, 5040
FbaI TGATCA 3 cut(s) 2383, 3994, 5991
FblI GTMKAC 3 cut(s) 2415, 3967, 7751
FriOI GRGCYC 2 cut(s) 6442, 7658
GlaI GCGC 1 cut(s) 2609
GsaI CCCAGC 3 cut(s) 210, 440, 7304
GsuI CTGGAG 6 cut(s) 2614, 2801, 2831, 4419, 6937, 7617
HapII CCGG 4 cut(s) 1831, 4382, 5491, 7743
HgaI GACGC 2 cut(s) 1850, 6959
HhaI GCGC 1 cut(s) 2610
Hin1I GRCGYC 1 cut(s) 2537
Hin6I GCGC 1 cut(s) 2608
HinP1I GCGC 1 cut(s) 2608
HincII GTYRAC 5 cut(s) 1020, 1608, 2155, 3968, 7495
HindII GTYRAC 5 cut(s) 1020, 1608, 2155, 3968, 7495
HindIII AAGCTT 4 cut(s) 1720, 2579, 6377, 6761
HpaII CCGG 4 cut(s) 1831, 4382, 5491, 7743
Hpy99I CGWCG 1 cut(s) 3969
HpyCH4IV ACGT 4 cut(s) 118, 2537, 3964, 6216
HpySE526I ACGT 4 cut(s) 118, 2537, 3964, 6216
Hsp92I GRCGYC 1 cut(s) 2537
HspAI GCGC 1 cut(s) 2608
Ksp22I TGATCA 3 cut(s) 2383, 3994, 5991
LguI GCTCTTC 2 cut(s) 2641, 7646
LmnI GCTCC 6 cut(s) 1552, 1631, 1849, 2781, 4384, 6967
MabI ACCWGGT 3 cut(s) 6036, 6943, 7570
MaeII ACGT 4 cut(s) 118, 2537, 3964, 6216
MfeI CAATTG 4 cut(s) 1667, 2438, 4119, 4335
MflI RGATCY 9 cut(s) 151, 1833, 2204, 2932, 3100, 4030, 4913, 4976, 5462
MhlI GDGCHC 5 cut(s) 22, 730, 5434, 6442, 7658
MlsI TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
MluNI TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
Mox20I TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
Mph1103I ATGCAT 3 cut(s) 1788, 3595, 5673
MroXI GAANNNNTTC 3 cut(s) 2522, 3212, 7326
MscI TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
MslI CAYNNNNRTG 5 cut(s) 371, 612, 3332, 7759, 7773
Msp20I TGGCCA 4 cut(s) 2402, 2902, 3609, 4958
MspA1I CMGCKG 3 cut(s) 3413, 5210, 6320
MspCI CTTAAG 1 cut(s) 7352
MspI CCGG 4 cut(s) 1831, 4382, 5491, 7743
MssI GTTTAAAC 1 cut(s) 6168
MunI CAATTG 4 cut(s) 1667, 2438, 4119, 4335
Mva1269I GAATGC 8 cut(s) 606, 1079, 1209, 1400, 1667, 1788, 6010, 7151
NcoI CCATGG 1 cut(s) 6791
NdeI CATATG 4 cut(s) 61, 1194, 4065, 5040
NlaIV GGNNCC 9 cut(s) 645, 1554, 1835, 2162, 2934, 3233, 3698, 3784, 4145
NmeAIII GCCGAG 2 cut(s) 3869, 7763
NsiI ATGCAT 3 cut(s) 1788, 3595, 5673
NspI RCATGY 9 cut(s) 436, 1643, 3371, 3396, 3798, 4532, 4850, 5357, 5900
NspV TTCGAA 2 cut(s) 4613, 4805
OliI CACNNNNGTG 2 cut(s) 371, 7759
PaeI GCATGC 2 cut(s) 4532, 5357
PagI TCATGA 2 cut(s) 1380, 3487
PaqCI CACCTGC 2 cut(s) 4459, 5636
PceI AGGCCT 4 cut(s) 1314, 2339, 3644, 6502
PciI ACATGT 2 cut(s) 1639, 3367
PciSI GCTCTTC 2 cut(s) 2641, 7646
PctI GAATGC 8 cut(s) 606, 1079, 1209, 1400, 1667, 1788, 6010, 7151
PdmI GAANNNNTTC 3 cut(s) 2522, 3212, 7326
PflMI CCANNNNNTGG 2 cut(s) 935, 1898
PfoI TCCNGGA 2 cut(s) 1836, 2778
PmeI GTTTAAAC 1 cut(s) 6168
PpuMI RGGWCCY 1 cut(s) 644
PscI ACATGT 2 cut(s) 1639, 3367
PshAI GACNNNNGTC 1 cut(s) 3194
PsiI TTATAA 3 cut(s) 1916, 2189, 3650
Psp124BI GAGCTC 1 cut(s) 7658
Psp5II RGGWCCY 1 cut(s) 644
PspEI GGTNACC 3 cut(s) 893, 1748, 7138
PspFI CCCAGC 3 cut(s) 206, 436, 7300
PspN4I GGNNCC 9 cut(s) 645, 1554, 1835, 2162, 2934, 3233, 3698, 3784, 4145
PspPPI RGGWCCY 1 cut(s) 644
PsrI GAACNNNNNNTAC 2 cut(s) 4644, 4676
PstI CTGCAG 2 cut(s) 5251, 6525
PstNI CAGNNNCTG 3 cut(s) 377, 3182, 6520
PsuI RGATCY 9 cut(s) 151, 1833, 2204, 2932, 3100, 4030, 4913, 4976, 5462
PvuII CAGCTG 2 cut(s) 3413, 6320
RseI CAYNNNNRTG 5 cut(s) 371, 612, 3332, 7759, 7773
SacI GAGCTC 1 cut(s) 7658
SalI GTCGAC 1 cut(s) 3966
SapI GCTCTTC 2 cut(s) 2641, 7646
ScaI AGTACT 4 cut(s) 2001, 2320, 4495, 6826
SduI GDGCHC 5 cut(s) 22, 730, 5434, 6442, 7658
SexAI ACCWGGT 3 cut(s) 6036, 6943, 7570
SfcI CTRYAG 8 cut(s) 1418, 4791, 5106, 5247, 6354, 6521, 7077, 7104
SfuI TTCGAA 2 cut(s) 4613, 4805
SinI GGWCC 6 cut(s) 644, 926, 1085, 1901, 2434, 5004
SmiMI CAYNNNNRTG 5 cut(s) 371, 612, 3332, 7759, 7773
SmlI CTYRAG 7 cut(s) 1698, 2076, 3971, 4565, 6504, 7352, 7412
SmoI CTYRAG 7 cut(s) 1698, 2076, 3971, 4565, 6504, 7352, 7412
SpeI ACTAGT 1 cut(s) 6055
SphI GCATGC 2 cut(s) 4532, 5357
SseBI AGGCCT 4 cut(s) 1314, 2339, 3644, 6502
SsiI CCGC 9 cut(s) 29, 604, 1152, 2005, 5208, 5588, 6464, 7360, 7825
SspI AATATT 9 cut(s) 1000, 1127, 2242, 2754, 2867, 4428, 5097, 6073, 6332
SstI GAGCTC 1 cut(s) 7658
StuI AGGCCT 4 cut(s) 1314, 2339, 3644, 6502
StyI CCWWGG 8 cut(s) 1489, 1500, 2340, 2403, 2600, 6791, 7278, 7515
TaiI ACGT 4 cut(s) 121, 2540, 3967, 6219
TauI GCSGC 2 cut(s) 607, 5210
TspGWI ACGGA 4 cut(s) 1779, 2705, 4253, 5524
Van91I CCANNNNNTGG 2 cut(s) 935, 1898
Vha464I CTTAAG 1 cut(s) 7352
VneI GTGCAC 2 cut(s) 18, 5430
VpaK11BI GGWCC 6 cut(s) 644, 926, 1085, 1901, 2434, 5004
XagI CCTNNNNNAGG 2 cut(s) 654, 3649
XbaI TCTAGA 2 cut(s) 3623, 5419
XceI RCATGY 9 cut(s) 436, 1643, 3371, 3396, 3798, 4532, 4850, 5357, 5900
XcmI CCANNNNNNNNNTGG 1 cut(s) 936
XmaJI CCTAGG 1 cut(s) 2600
XmiI GTMKAC 3 cut(s) 2415, 3967, 7751
XmnI GAANNNNTTC 3 cut(s) 2522, 3212, 7326
ZraI GACGTC 1 cut(s) 2538
ZrmI AGTACT 4 cut(s) 2001, 2320, 4495, 6826
Zsp2I ATGCAT 3 cut(s) 1788, 3595, 5673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.