MD04G1231500.v1.1

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000145
Physical Location & Seq
Forward (+)
31101802 .. 31104799
2998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1231500.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCCAGGGGTTACATGATAATCGTAGTGATCATGAATACAGACCCATATATTATTATCAGGGCAAGAAATCTTCTTCACCTTCTCTCAATTGGAGTTCCTGCATCTCAGGCATTAGAAGTTCTGAATGGAAAGATATGCGATGTCATCGACGTAGGGTTTAAAAGGAATGGACTTTGCTCAAAATTTGGTATCAAAAAGGATCAATATGTTGAACGACGACATCTTCTCCGTGGCTCCTTGCAGGCAATGGCAGATCTAACAGCCACTCAGATTTTCTTGCTTGGTGAAGTCGTTGCTGCTATAGGGGGTTCATCTCTTGGATTAAACATATTCAGGAAGATTGTGGAAGACTGTATAGTCCATAAAGTGCCTCCTGCATACCATATCAAGAATTTTAAGATGAGAAAACAAGTGATGAAGGATCTCGAGGCTATGCGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.58

Weight (kDa)

9.75

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 14 - 43 3.4e-06 Krr1 KH1 domain
KH_KRR1_2nd PF21800 65 - 141 1.7e-10 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08420
fragaria_vesca FvH4_1g11160 FvH4_2g08143 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36460 FvH4_3g36460 FvH4_3g45301 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_5g09160 FvH4_5g09161 FvH4_5g09380 FvH4_5g22900 FvH4_6g42520 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550
malus_domestica MD02G1015700.v1.1 MD02G1058900.v1.1 MD02G1059000.v1.1 MD03G1170900.v1.1 MD04G1231500.v1.1 MD13G1044300.v1.1 MD16G1045100.v1.1
prunus_persica Prupe.1G308200_v2.0.a1 Prupe.2G125300_v2.0.a1 Prupe.6G307800_v2.0.a1 Prupe.6G351700_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1
pyrus_communis pycom02g01420 pycom02g04810 pycom03g12790 pycom03g12800 pycom12g17840 pycom12g17850 pycom12g17860 pycom13g03890 pycom16g03970
rosa_chinensis RchiOBHm_Chr2g0090081 RchiOBHm_Chr3g0469061 RchiOBHm_Chr3g0469081 RchiOBHm_Chr4g0435531 RchiOBHm_Chr5g0014021 RchiOBHm_Chr5g0014031 RchiOBHm_Chr5g0014041 RchiOBHm_Chr7g0190151 RchiOBHm_Chr7g0190161 RchiOBHm_Chr7g0190171 RchiOBHm_Chr7g0210441
rosa_laevigata RLG00000003683 RLG00000003687 RLG00000003688 RLG00000004561 RLG00000004643 RLG00000016083 RLG00000021085 RLG00000024381 RLG00000032065 RLG00000032067 RLG00000032071 RLG00000032072 RLG00000032075
rosa_multiflora Rmu_co8284349.1_g000001 Rmu_co8324735.1_g000001 Rmu_co8355915.1_g000001 Rmu_co8388905.1_g000001 Rmu_sc0000302.1_g000041 Rmu_sc0000302.1_g000050 Rmu_sc0001940.1_g000034 Rmu_sc0003807.1_g000011 Rmu_sc0005803.1_g000001 Rmu_sc0005803.1_g000007 Rmu_sc0007139.1_g000007 Rmu_sc0007767.1_g000001 Rmu_sc0007767.1_g000003 Rmu_sc0007767.1_g000006 Rmu_sc0007933.1_g000005 Rmu_sc0008587.1_g000004 Rmu_sc0008968.1_g000004 Rmu_sc0018491.1_g000002 Rmu_sc0022420.1_g000001 Rmu_sc0027700.1_g000001 Rmu_sc0036218.1_g000001
rosa_roxburghii Rroxscaffold_1G00062590 Rroxscaffold_1G00062600 Rroxscaffold_1G00062620 Rroxscaffold_1G00062630 Rroxscaffold_1G00062640 Rroxscaffold_1G00062670 Rroxscaffold_1G00062680 Rroxscaffold_2G00091430 Rroxscaffold_2G00151410 Rroxscaffold_3G00245340 Rroxscaffold_3G00255390 Rroxscaffold_3G00264820 Rroxscaffold_3G00264830 Rroxscaffold_3G00265870 Rroxscaffold_5G00355140 Rroxscaffold_5G00376760 Rroxscaffold_6G00412230
rosa_rugosa Rorug02G0005200 Rorug02G0468300 Rorug02G0468300 Rorug02G0532400 Rorug03G0098900 Rorug03G0099000 Rorug03G0099400 Rorug04G0286000 Rorug05G0014400 Rorug05G0014500 Rorug05G0014600 Rorug05G0014600 Rorug05G0014600 Rorug05G0014800 Rorug05G0014900 Rorug05G0015000 Rorug05G0015100 Rorug05G0015100 Rorug05G0441300 Rorug06G0495300 Rorug06G0502600 Rorug06G0502700 Rorug07G0062300
rosa_samantha Rh2AG050900 Rh2BG049600 Rh2CG051800 Rh2CG234600 Rh2DG051100 Rh3BG171300 Rh3BG171800 Rh3CG336700 Rh3DG203600 Rh3DG203800 Rh4AG341600 Rh4AG341700 Rh4BG349900 Rh4CG364600 Rh4DG344200 Rh5AG108800 Rh5AG108900 Rh5AG109000 Rh5AG109100 Rh5BG105500 Rh5CG117300 Rh5CG117500 Rh5CG117600 Rh5DG104200 Rh5DG104400 Rh5DG104500 Rh5DG104600 Rh7BG102300 Rh7BG110600 Rh7BG110700 Rh7BG118900 Rh7CG103600 Rh7CG112800 Rh7CG113000 Rh7CG200300 Rh7CG271100 Rh7DG111300 Rh7DG111400 Rh7DG194400 Rh7DG261800 Rh7DG261900
rosa_wichuraiana Rw0G014550 Rw0G014580 Rw2G004580 Rw3G013980 Rw3G013990 Rw3G014000 Rw3G014020 Rw4G029810 Rw5G009500 Rw5G009510 Rw5G009520 Rw5G009530 Rw7G008640 Rw7G009350 Rw7G021680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 445
AasI GACNNNNNNGTC 1 cut(s) 359
AclWI GGATC 2 cut(s) 210, 432
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 185, 394
AgsI TTSAA 1 cut(s) 215
AjnI CCWGG 1 cut(s) 5
AlwI GGATC 2 cut(s) 210, 432
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 2 cut(s) 185, 394
AsuHPI GGTGA 2 cut(s) 71, 299
AvaI CYCGRG 1 cut(s) 428
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 7
BclI TGATCA 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 303
BglII AGATCT 1 cut(s) 256
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 7
BmeT110I CYCGRG 1 cut(s) 428
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 7
BmsI GCATC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 6, 232
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 7
BseDI CCNNGG 2 cut(s) 6, 232
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 122, 284
BseXI GCAGC 1 cut(s) 286
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 428
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 428
Bsp143I GATC 4 cut(s) 30, 202, 256, 424
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 121, 283
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 2 cut(s) 210, 432
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 6, 232
BssMI GATC 4 cut(s) 30, 202, 256, 424
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 108, 270
BstDSI CCRYGG 1 cut(s) 232
BstKTI GATC 4 cut(s) 33, 205, 259, 427
BstMBI GATC 4 cut(s) 30, 202, 256, 424
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 5
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 2 cut(s) 256, 424
BstYI RGATCY 2 cut(s) 256, 424
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 232
BtgZI GCGATG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 1 cut(s) 33
CviAII CATG 2 cut(s) 16, 34
CviJI RGCY 4 cut(s) 5, 237, 266, 434
CviKI_1 RGCY 4 cut(s) 5, 237, 266, 434
DdeI CTNAG 2 cut(s) 108, 270
DpnI GATC 4 cut(s) 32, 204, 258, 426
DpnII GATC 4 cut(s) 30, 202, 256, 424
DraI TTTAAA 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 3
Eco88I CYCGRG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 5
FaeI CATG 2 cut(s) 19, 37
FatI CATG 2 cut(s) 15, 33
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 300
GluI GCNGC 1 cut(s) 300
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 19, 37
HphI GGTGA 2 cut(s) 71, 299
Hpy188I TCNGA 2 cut(s) 126, 273
Hpy188III TCNNGA 4 cut(s) 34, 337, 391, 428
Hpy99I CGWCG 2 cut(s) 155, 222
HpyAV CCTTC 2 cut(s) 92, 415
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 3 cut(s) 104, 244, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 108, 270
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 2 cut(s) 19, 37
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 4 cut(s) 30, 202, 256, 424
LmnI GCTCC 1 cut(s) 242
LpnPI CCDG 7 cut(s) 19, 46, 95, 114, 230, 322, 390
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 113
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 32, 204, 258, 426
MboI GATC 4 cut(s) 30, 202, 256, 424
MboII GAAGA 5 cut(s) 65, 68, 218, 352, 362
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 2 cut(s) 256, 424
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 90, 185, 394
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 384, 424
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 162, 326, 399
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 7
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 4 cut(s) 30, 202, 256, 424
NlaIII CATG 2 cut(s) 19, 37
NlaIV GGNNCC 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 301
PsiI TTATAA 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 5
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 238
PsuI RGATCY 2 cut(s) 256, 424
SaqAI TTAA 3 cut(s) 162, 326, 399
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 4 cut(s) 30, 202, 256, 424
ScrFI CCNGG 1 cut(s) 7
SetI ASST 2 cut(s) 84, 156
SfaNI GCATC 1 cut(s) 113
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 428
SlaI CTCGAG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 3 cut(s) 90, 185, 394
StyD4I CCNGG 1 cut(s) 5
TaaI ACNGT 1 cut(s) 356
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 150, 429
TasI AATT 3 cut(s) 90, 185, 394
Tru1I TTAA 3 cut(s) 162, 326, 399
Tru9I TTAA 3 cut(s) 162, 326, 399
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 50, 303, 434
TspGWI ACGGA 1 cut(s) 221
XapI RAATTY 2 cut(s) 185, 394
XhoI CTCGAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.