Rw5G009530

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000145
Physical Location & Seq
Reverse (-)
10258059 .. 10260349
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009530.1

Sequence Viewer

Length: 564 bp
ATGTCGGAAGATTACCCAAACGCCGATTACCCAAACGCCATTGTTCTGCAGCTCGAGGAGGCTCTGATGGTCCAAGATGAAATTGAAGTCGTCTCTTACATCAAGTTCTACGATCGATTCCTTGACACAGATTTGCTGCAAAAAAGTTGGCCTATAGTGGAGTCGTGTTTGAGCAAGCATGGCGTTTTGTGCACACTGGATCTGGCGATACGAACGCTGGATTGCAGTTGCCAACATGAGATCATCAAGATTGGGAATCAAGAAGGGGGGATTTGCAACATATTTGGGATCAGTAAGGTGGGTATTACATGTTTCCTTCCTCTCTTTATTGGACTTTTTGAACTTACTGGCTGTGGCGTTTTTCTTAAGGGAAATACCATTGCTGTTATTGGTCCACTGCAAGGAATAAAGACGATAACAAAGATTGTGGAAGACTGCATCGCTCACAATGTGCCTCCTGCACCCCGTGTGCGGAGGATTAAAAAGAAGACTCAACTGATGAAGGATGCGAGGATTAAAATGACAAGTGAAGTGATGATGAGTCTCGAGGCTTTTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.89

Weight (kDa)

5.81

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_2nd PF21800 115 - 168 1.1e-10 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08420
fragaria_vesca FvH4_1g11160 FvH4_2g08143 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36460 FvH4_3g36460 FvH4_3g45301 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_5g09160 FvH4_5g09161 FvH4_5g09380 FvH4_5g22900 FvH4_6g42520 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550
malus_domestica MD02G1015700.v1.1 MD02G1058900.v1.1 MD02G1059000.v1.1 MD03G1170900.v1.1 MD04G1231500.v1.1 MD13G1044300.v1.1 MD16G1045100.v1.1
prunus_persica Prupe.1G308200_v2.0.a1 Prupe.2G125300_v2.0.a1 Prupe.6G307800_v2.0.a1 Prupe.6G351700_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1
pyrus_communis pycom02g01420 pycom02g04810 pycom03g12790 pycom03g12800 pycom12g17840 pycom12g17850 pycom12g17860 pycom13g03890 pycom16g03970
rosa_chinensis RchiOBHm_Chr2g0090081 RchiOBHm_Chr3g0469061 RchiOBHm_Chr3g0469081 RchiOBHm_Chr4g0435531 RchiOBHm_Chr5g0014021 RchiOBHm_Chr5g0014031 RchiOBHm_Chr5g0014041 RchiOBHm_Chr7g0190151 RchiOBHm_Chr7g0190161 RchiOBHm_Chr7g0190171 RchiOBHm_Chr7g0210441
rosa_laevigata RLG00000003683 RLG00000003687 RLG00000003688 RLG00000004561 RLG00000004643 RLG00000016083 RLG00000021085 RLG00000024381 RLG00000032065 RLG00000032067 RLG00000032071 RLG00000032072 RLG00000032075
rosa_multiflora Rmu_co8284349.1_g000001 Rmu_co8324735.1_g000001 Rmu_co8355915.1_g000001 Rmu_co8388905.1_g000001 Rmu_sc0000302.1_g000041 Rmu_sc0000302.1_g000050 Rmu_sc0001940.1_g000034 Rmu_sc0003807.1_g000011 Rmu_sc0005803.1_g000001 Rmu_sc0005803.1_g000007 Rmu_sc0007139.1_g000007 Rmu_sc0007767.1_g000001 Rmu_sc0007767.1_g000003 Rmu_sc0007767.1_g000006 Rmu_sc0007933.1_g000005 Rmu_sc0008587.1_g000004 Rmu_sc0008968.1_g000004 Rmu_sc0018491.1_g000002 Rmu_sc0022420.1_g000001 Rmu_sc0027700.1_g000001 Rmu_sc0036218.1_g000001
rosa_roxburghii Rroxscaffold_1G00062590 Rroxscaffold_1G00062600 Rroxscaffold_1G00062620 Rroxscaffold_1G00062630 Rroxscaffold_1G00062640 Rroxscaffold_1G00062670 Rroxscaffold_1G00062680 Rroxscaffold_2G00091430 Rroxscaffold_2G00151410 Rroxscaffold_3G00245340 Rroxscaffold_3G00255390 Rroxscaffold_3G00264820 Rroxscaffold_3G00264830 Rroxscaffold_3G00265870 Rroxscaffold_5G00355140 Rroxscaffold_5G00376760 Rroxscaffold_6G00412230
rosa_rugosa Rorug02G0005200 Rorug02G0468300 Rorug02G0468300 Rorug02G0532400 Rorug03G0098900 Rorug03G0099000 Rorug03G0099400 Rorug04G0286000 Rorug05G0014400 Rorug05G0014500 Rorug05G0014600 Rorug05G0014600 Rorug05G0014600 Rorug05G0014800 Rorug05G0014900 Rorug05G0015000 Rorug05G0015100 Rorug05G0015100 Rorug05G0441300 Rorug06G0495300 Rorug06G0502600 Rorug06G0502700 Rorug07G0062300
rosa_samantha Rh2AG050900 Rh2BG049600 Rh2CG051800 Rh2CG234600 Rh2DG051100 Rh3BG171300 Rh3BG171800 Rh3CG336700 Rh3DG203600 Rh3DG203800 Rh4AG341600 Rh4AG341700 Rh4BG349900 Rh4CG364600 Rh4DG344200 Rh5AG108800 Rh5AG108900 Rh5AG109000 Rh5AG109100 Rh5BG105500 Rh5CG117300 Rh5CG117500 Rh5CG117600 Rh5DG104200 Rh5DG104400 Rh5DG104500 Rh5DG104600 Rh7BG102300 Rh7BG110600 Rh7BG110700 Rh7BG118900 Rh7CG103600 Rh7CG112800 Rh7CG113000 Rh7CG200300 Rh7CG271100 Rh7DG111300 Rh7DG111400 Rh7DG194400 Rh7DG261800 Rh7DG261900
rosa_wichuraiana Rw0G014550 Rw0G014580 Rw2G004580 Rw3G013980 Rw3G013990 Rw3G014000 Rw3G014020 Rw4G029810 Rw5G009500 Rw5G009510 Rw5G009520 Rw5G009530 Rw7G008640 Rw7G009350 Rw7G021680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 472
AclWI GGATC 2 cut(s) 207, 296
AdeI CACNNNGTG 2 cut(s) 451, 467
AfiI CCNNNNNNNGG 2 cut(s) 401, 471
AflII CTTAAG 1 cut(s) 365
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 2 cut(s) 86, 341
AluBI AGCT 1 cut(s) 52
AluI AGCT 1 cut(s) 52
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 2 cut(s) 97, 548
Alw44I GTGCAC 1 cut(s) 190
AlwI GGATC 2 cut(s) 207, 296
Ama87I CYCGRG 2 cut(s) 53, 545
AoxI GGCC 1 cut(s) 149
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 49, 136
AspS9I GGNCC 2 cut(s) 70, 392
AvaI CYCGRG 2 cut(s) 53, 545
AvaII GGWCC 2 cut(s) 70, 392
BaeGI GKGCMC 1 cut(s) 194
BbsI GAAGAC 2 cut(s) 438, 494
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 61, 123
BccI CCATC 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 97, 548
BfmI CTRYAG 2 cut(s) 47, 153
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 2 cut(s) 50, 137
BlsI GCNGC 2 cut(s) 51, 138
Bme18I GGWCC 2 cut(s) 70, 392
BmeT110I CYCGRG 2 cut(s) 53, 545
BmgT120I GGNCC 2 cut(s) 70, 392
BmsI GCATC 2 cut(s) 447, 496
BpiI GAAGAC 2 cut(s) 438, 494
Bsa29I ATCGAT 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 471
Bse1I ACTGG 2 cut(s) 201, 352
Bse3DI GCAATG 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 115
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 401, 471
BseMI GCAATG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 201, 352
BseRI GAGGAG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 61, 123
BsgI GTGCAG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 151
BshVI ATCGAT 1 cut(s) 115
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 2 cut(s) 53, 545
BslI CCNNNNNNNGG 2 cut(s) 401, 471
BsmAI GTCTC 2 cut(s) 97, 548
BsmBI CGTCTC 1 cut(s) 97
BsnI GGCC 1 cut(s) 151
BsoBI CYCGRG 2 cut(s) 53, 545
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 4 cut(s) 112, 199, 240, 288
BspACI CCGC 1 cut(s) 472
BspANI GGCC 1 cut(s) 151
BspDI ATCGAT 1 cut(s) 115
BspMAI CTGCAG 1 cut(s) 51
BspPI GGATC 2 cut(s) 207, 296
BspTI CTTAAG 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 2 cut(s) 201, 352
BssMI GATC 4 cut(s) 112, 199, 240, 288
BstAFI CTTAAG 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 176
BstF5I GGATG 1 cut(s) 511
BstKTI GATC 4 cut(s) 115, 202, 243, 291
BstMAI GTCTC 2 cut(s) 97, 548
BstMBI GATC 4 cut(s) 112, 199, 240, 288
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 180, 189
BstNSI RCATGY 1 cut(s) 312
BstSFI CTRYAG 2 cut(s) 47, 153
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 61, 123
BstV2I GAAGAC 2 cut(s) 438, 494
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 115
BsuRI GGCC 1 cut(s) 151
BsuTUI ATCGAT 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 424
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 2 cut(s) 194, 395
Cac8I GCNNGC 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 70, 392
ClaI ATCGAT 1 cut(s) 115
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 4 cut(s) 179, 236, 309, 557
CviJI RGCY 5 cut(s) 52, 62, 151, 351, 551
CviKI_1 RGCY 5 cut(s) 52, 62, 151, 351, 551
DpnI GATC 4 cut(s) 114, 201, 242, 290
DpnII GATC 4 cut(s) 112, 199, 240, 288
DraIII CACNNNGTG 2 cut(s) 451, 467
Eco47I GGWCC 2 cut(s) 70, 392
Eco88I CYCGRG 2 cut(s) 53, 545
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 4 cut(s) 182, 239, 312, 560
FaiI YATR 6 cut(s) 155, 180, 237, 281, 310, 558
FatI CATG 4 cut(s) 178, 235, 308, 556
Fnu4HI GCNGC 2 cut(s) 50, 137
FokI GGATG 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 50, 137
GluI GCNGC 2 cut(s) 50, 137
HaeIII GGCC 1 cut(s) 151
Hin1II CATG 4 cut(s) 182, 239, 312, 560
HinfI GANTC 5 cut(s) 117, 161, 256, 490, 541
Hpy166II GTNNAC 2 cut(s) 192, 395
Hpy188I TCNGA 3 cut(s) 7, 66, 563
Hpy188III TCNNGA 3 cut(s) 247, 260, 545
Hpy8I GTNNAC 2 cut(s) 192, 395
HpyAV CCTTC 3 cut(s) 257, 326, 496
HpyCH4V TGCA 8 cut(s) 49, 139, 192, 225, 276, 400, 438, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 189
Hsp92II CATG 4 cut(s) 182, 239, 312, 560
Kzo9I GATC 4 cut(s) 112, 199, 240, 288
LpnPI CCDG 5 cut(s) 182, 188, 203, 333, 471
Lsp1109I GCAGC 2 cut(s) 61, 123
LweI GCATC 2 cut(s) 447, 496
MalI GATC 4 cut(s) 114, 201, 242, 290
MboI GATC 4 cut(s) 112, 199, 240, 288
MboII GAAGA 3 cut(s) 20, 443, 499
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 1 cut(s) 81
MlyI GAGTC 3 cut(s) 170, 484, 550
MnlI CCTC 7 cut(s) 49, 52, 330, 465, 468, 504, 541
MseI TTAA 3 cut(s) 366, 480, 516
MspCI CTTAAG 1 cut(s) 365
MwoI GCNNNNNNNGC 2 cut(s) 180, 189
NdeII GATC 4 cut(s) 112, 199, 240, 288
NlaIII CATG 4 cut(s) 182, 239, 312, 560
NspI RCATGY 1 cut(s) 312
PaeR7I CTCGAG 2 cut(s) 53, 545
PciI ACATGT 1 cut(s) 308
PfeI GAWTC 2 cut(s) 117, 256
PkrI GCNGC 2 cut(s) 51, 138
Ple19I CGATCG 1 cut(s) 115
PleI GAGTC 3 cut(s) 169, 484, 549
PpsI GAGTC 3 cut(s) 169, 484, 549
PscI ACATGT 1 cut(s) 308
PspPI GGNCC 2 cut(s) 70, 392
PspXI VCTCGAGB 1 cut(s) 53
PstI CTGCAG 1 cut(s) 51
PsuI RGATCY 1 cut(s) 199
PvuI CGATCG 1 cut(s) 115
SaqAI TTAA 3 cut(s) 366, 480, 516
SatI GCNGC 2 cut(s) 50, 137
Sau3AI GATC 4 cut(s) 112, 199, 240, 288
Sau96I GGNCC 2 cut(s) 70, 392
SchI GAGTC 3 cut(s) 170, 484, 550
SduI GDGCHC 1 cut(s) 194
SetI ASST 2 cut(s) 54, 300
SfaNI GCATC 2 cut(s) 447, 496
SfcI CTRYAG 2 cut(s) 47, 153
Sfr274I CTCGAG 2 cut(s) 53, 545
SinI GGWCC 2 cut(s) 70, 392
SlaI CTCGAG 2 cut(s) 53, 545
SmlI CTYRAG 3 cut(s) 53, 365, 545
SmoI CTYRAG 3 cut(s) 53, 365, 545
Sse9I AATT 1 cut(s) 81
SsiI CCGC 1 cut(s) 472
TaqI TCGA 3 cut(s) 54, 115, 546
TasI AATT 1 cut(s) 81
TfiI GAWTC 2 cut(s) 117, 256
Tru1I TTAA 3 cut(s) 366, 480, 516
Tru9I TTAA 3 cut(s) 366, 480, 516
TscAI CASTG 2 cut(s) 201, 402
TseI GCWGC 2 cut(s) 49, 136
TspDTI ATGAA 3 cut(s) 93, 515, 545
TspRI CASTG 2 cut(s) 201, 402
Vha464I CTTAAG 1 cut(s) 365
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 70, 392
XceI RCATGY 1 cut(s) 312
XhoI CTCGAG 2 cut(s) 53, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.