Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 61, 131 |
| AcsI | RAATTY | 1 cut(s) | 207 |
| AfaI | GTAC | 2 cut(s) | 119, 183 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 242 |
| AgsI | TTSAA | 1 cut(s) | 191 |
| AluBI | AGCT | 1 cut(s) | 157 |
| AluI | AGCT | 1 cut(s) | 157 |
| AlwI | GGATC | 2 cut(s) | 61, 131 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 184 |
| Ama87I | CYCGRG | 1 cut(s) | 306 |
| ApoI | RAATTY | 1 cut(s) | 207 |
| AsuC2I | CCSGG | 2 cut(s) | 307, 308 |
| AsuHPI | GGTGA | 1 cut(s) | 118 |
| AvaI | CYCGRG | 1 cut(s) | 306 |
| BbsI | GAAGAC | 2 cut(s) | 279, 335 |
| BcnI | CCSGG | 2 cut(s) | 307, 308 |
| Bme1390I | CCNGG | 2 cut(s) | 307, 308 |
| BmeT110I | CYCGRG | 1 cut(s) | 306 |
| BmrFI | CCNGG | 2 cut(s) | 307, 308 |
| BpiI | GAAGAC | 2 cut(s) | 279, 335 |
| BpuMI | CCSGG | 2 cut(s) | 307, 308 |
| BsaJI | CCNNGG | 3 cut(s) | 241, 305, 306 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 242 |
| Bse1I | ACTGG | 1 cut(s) | 67 |
| Bse3DI | GCAATG | 1 cut(s) | 279 |
| BseDI | CCNNGG | 3 cut(s) | 241, 305, 306 |
| BseGI | GGATG | 1 cut(s) | 351 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 242 |
| BseMI | GCAATG | 1 cut(s) | 279 |
| BseNI | ACTGG | 1 cut(s) | 67 |
| BseRI | GAGGAG | 1 cut(s) | 288 |
| BsiHKCI | CYCGRG | 1 cut(s) | 306 |
| BsiSI | CCGG | 1 cut(s) | 307 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 242 |
| BsoBI | CYCGRG | 1 cut(s) | 306 |
| Bsp143I | GATC | 4 cut(s) | 5, 53, 75, 123 |
| BspHI | TCATGA | 1 cut(s) | 286 |
| BspPI | GGATC | 2 cut(s) | 61, 131 |
| BsrDI | GCAATG | 1 cut(s) | 279 |
| BsrI | ACTGG | 1 cut(s) | 67 |
| BssECI | CCNNGG | 3 cut(s) | 241, 305, 306 |
| BssMI | GATC | 4 cut(s) | 5, 53, 75, 123 |
| BssT1I | CCWWGG | 1 cut(s) | 241 |
| Bst4CI | ACNGT | 1 cut(s) | 186 |
| BstC8I | GCNNGC | 1 cut(s) | 166 |
| BstF5I | GGATG | 1 cut(s) | 351 |
| BstKTI | GATC | 4 cut(s) | 8, 56, 78, 126 |
| BstMBI | GATC | 4 cut(s) | 5, 53, 75, 123 |
| BstSCI | CCNGG | 2 cut(s) | 305, 306 |
| BstV2I | GAAGAC | 2 cut(s) | 279, 335 |
| BtsCI | GGATG | 1 cut(s) | 351 |
| Cac8I | GCNNGC | 1 cut(s) | 166 |
| CaiI | CAGNNNCTG | 1 cut(s) | 184 |
| CciI | TCATGA | 1 cut(s) | 286 |
| Cfr9I | CCCGGG | 1 cut(s) | 306 |
| Csp6I | GTAC | 2 cut(s) | 118, 182 |
| CviAII | CATG | 1 cut(s) | 287 |
| CviJI | RGCY | 4 cut(s) | 106, 157, 164, 168 |
| CviKI_1 | RGCY | 4 cut(s) | 106, 157, 164, 168 |
| CviQI | GTAC | 2 cut(s) | 118, 182 |
| DpnI | GATC | 4 cut(s) | 7, 55, 77, 125 |
| DpnII | GATC | 4 cut(s) | 5, 53, 75, 123 |
| Eco130I | CCWWGG | 1 cut(s) | 241 |
| Eco88I | CYCGRG | 1 cut(s) | 306 |
| EcoT14I | CCWWGG | 1 cut(s) | 241 |
| ErhI | CCWWGG | 1 cut(s) | 241 |
| FaeI | CATG | 1 cut(s) | 290 |
| FaiI | YATR | 4 cut(s) | 46, 171, 254, 288 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 90, 122 |
| FatI | CATG | 1 cut(s) | 286 |
| HapII | CCGG | 1 cut(s) | 307 |
| Hin1II | CATG | 1 cut(s) | 290 |
| HindIII | AAGCTT | 1 cut(s) | 155 |
| HinfI | GANTC | 3 cut(s) | 21, 91, 331 |
| HpaII | CCGG | 1 cut(s) | 307 |
| HphI | GGTGA | 1 cut(s) | 118 |
| Hpy188III | TCNNGA | 5 cut(s) | 12, 25, 82, 95, 287 |
| HpyAV | CCTTC | 4 cut(s) | 22, 92, 255, 336 |
| HpyCH4III | ACNGT | 1 cut(s) | 186 |
| HpyCH4V | TGCA | 4 cut(s) | 41, 111, 146, 279 |
| Hsp92II | CATG | 1 cut(s) | 290 |
| Kzo9I | GATC | 4 cut(s) | 5, 53, 75, 123 |
| LpnPI | CCDG | 6 cut(s) | 80, 146, 150, 164, 183, 320 |
| MalI | GATC | 4 cut(s) | 7, 55, 77, 125 |
| MboI | GATC | 4 cut(s) | 5, 53, 75, 123 |
| MboII | GAAGA | 2 cut(s) | 284, 340 |
| MluCI | AATT | 3 cut(s) | 137, 207, 222 |
| MlyI | GAGTC | 1 cut(s) | 325 |
| MnlI | CCTC | 4 cut(s) | 95, 144, 306, 309 |
| MspI | CCGG | 1 cut(s) | 307 |
| MspR9I | CCNGG | 2 cut(s) | 307, 308 |
| NciI | CCSGG | 2 cut(s) | 307, 308 |
| NdeII | GATC | 4 cut(s) | 5, 53, 75, 123 |
| NlaIII | CATG | 1 cut(s) | 290 |
| PagI | TCATGA | 1 cut(s) | 286 |
| PfeI | GAWTC | 2 cut(s) | 21, 91 |
| PleI | GAGTC | 1 cut(s) | 325 |
| PpsI | GAGTC | 1 cut(s) | 325 |
| PstNI | CAGNNNCTG | 1 cut(s) | 184 |
| RsaI | GTAC | 2 cut(s) | 119, 183 |
| RsaNI | GTAC | 2 cut(s) | 118, 182 |
| Sau3AI | GATC | 4 cut(s) | 5, 53, 75, 123 |
| SchI | GAGTC | 1 cut(s) | 325 |
| ScrFI | CCNGG | 2 cut(s) | 307, 308 |
| SetI | ASST | 3 cut(s) | 159, 183, 202 |
| SmaI | CCCGGG | 1 cut(s) | 308 |
| Sse9I | AATT | 3 cut(s) | 137, 207, 222 |
| StyD4I | CCNGG | 2 cut(s) | 305, 306 |
| StyI | CCWWGG | 1 cut(s) | 241 |
| TaaI | ACNGT | 1 cut(s) | 186 |
| TasI | AATT | 3 cut(s) | 137, 207, 222 |
| TfiI | GAWTC | 2 cut(s) | 21, 91 |
| TspDTI | ATGAA | 1 cut(s) | 335 |
| TspGWI | ACGGA | 1 cut(s) | 163 |
| TspMI | CCCGGG | 1 cut(s) | 306 |
| XapI | RAATTY | 1 cut(s) | 207 |
| XmaI | CCCGGG | 1 cut(s) | 306 |