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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 23 |
| AcsI | RAATTY | 1 cut(s) | 98 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 22, 34 |
| AluBI | AGCT | 1 cut(s) | 131 |
| AluI | AGCT | 1 cut(s) | 131 |
| Alw26I | GTCTC | 1 cut(s) | 79 |
| ApoI | RAATTY | 1 cut(s) | 98 |
| AspS9I | GGNCC | 1 cut(s) | 25 |
| AvaII | GGWCC | 1 cut(s) | 25 |
| BcoDI | GTCTC | 1 cut(s) | 79 |
| Bme18I | GGWCC | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 1 cut(s) | 25 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 22, 34 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 22, 34 |
| BseMII | CTCAG | 2 cut(s) | 39, 118 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 22, 34 |
| BsmAI | GTCTC | 1 cut(s) | 79 |
| BsmBI | CGTCTC | 1 cut(s) | 79 |
| Bsp143I | GATC | 1 cut(s) | 30 |
| BspACI | CCGC | 1 cut(s) | 23 |
| BspCNI | CTCAG | 2 cut(s) | 40, 117 |
| BssMI | GATC | 1 cut(s) | 30 |
| BstC8I | GCNNGC | 1 cut(s) | 140 |
| BstDEI | CTNAG | 2 cut(s) | 48, 104 |
| BstKTI | GATC | 1 cut(s) | 33 |
| BstMAI | GTCTC | 1 cut(s) | 79 |
| BstMBI | GATC | 1 cut(s) | 30 |
| Cac8I | GCNNGC | 1 cut(s) | 140 |
| Cfr13I | GGNCC | 1 cut(s) | 25 |
| CviJI | RGCY | 3 cut(s) | 131, 138, 142 |
| CviKI_1 | RGCY | 3 cut(s) | 131, 138, 142 |
| DdeI | CTNAG | 2 cut(s) | 48, 104 |
| DpnI | GATC | 1 cut(s) | 32 |
| DpnII | GATC | 1 cut(s) | 30 |
| Eco47I | GGWCC | 1 cut(s) | 25 |
| EcoRI | GAATTC | 1 cut(s) | 98 |
| Esp3I | CGTCTC | 1 cut(s) | 79 |
| FaiI | YATR | 2 cut(s) | 145, 160 |
| HindIII | AAGCTT | 1 cut(s) | 129 |
| Hpy188I | TCNGA | 2 cut(s) | 49, 107 |
| Hpy188III | TCNNGA | 1 cut(s) | 55 |
| Hpy99I | CGWCG | 1 cut(s) | 98 |
| HpyCH4V | TGCA | 2 cut(s) | 120, 158 |
| HpyF3I | CTNAG | 2 cut(s) | 48, 104 |
| Kzo9I | GATC | 1 cut(s) | 30 |
| LpnPI | CCDG | 3 cut(s) | 41, 120, 124 |
| MalI | GATC | 1 cut(s) | 32 |
| MboI | GATC | 1 cut(s) | 30 |
| MluCI | AATT | 2 cut(s) | 98, 111 |
| MnlI | CCTC | 3 cut(s) | 28, 113, 118 |
| NdeII | GATC | 1 cut(s) | 30 |
| PspPI | GGNCC | 1 cut(s) | 25 |
| Sau3AI | GATC | 1 cut(s) | 30 |
| Sau96I | GGNCC | 1 cut(s) | 25 |
| SetI | ASST | 3 cut(s) | 39, 133, 157 |
| SgeI | CNNG | 8 cut(s) | 40, 46, 67, 97, 129, 133, 147, 151 |
| SinI | GGWCC | 1 cut(s) | 25 |
| Sse9I | AATT | 2 cut(s) | 98, 111 |
| SsiI | CCGC | 1 cut(s) | 23 |
| TaqI | TCGA | 2 cut(s) | 33, 54 |
| TasI | AATT | 2 cut(s) | 98, 111 |
| TspGWI | ACGGA | 1 cut(s) | 137 |
| VpaK11BI | GGWCC | 1 cut(s) | 25 |
| XapI | RAATTY | 1 cut(s) | 98 |