Rw5G009520

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000145
Physical Location & Seq
Reverse (-)
10223755 .. 10226683
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009520.1

Sequence Viewer

Length: 675 bp
ATGGAGAACAGCAACCCAAAAGCGGACCAGATCGAGGTGGAAGTGTCTGAGTTCGAGAATGATTTTGATGTCGTCTCTTACACCAAGTTCTACAACGAATTCCTGAATGTAGATTTGCTGCAAGAAAGTTGGCCAAAAGTGGAATCTGCTTTAGTAGAGTTCGGCGTTTTATGCACCCTGGATGTGGTTGAGGGTAAAATGAAAGTTGCAAGAGCCACAAGGGATCAAGACCCAAATATTATTCTCAGGGCTATGGATGTTTTAGAGCTTTTGTCAAGAAGTGTTCCGGCAGAATGGGCAATACGAGTACTGGATTGCAGTTTCCAGTACGACATCATCAAGATTGGGAATCAAGAAGAGGGGATTTGCAATATGTACGGGATCACTAAGGAGCAATTTCTTGCACGGAGGAAGCTTCTGGCTGGCTATGTCCTAAAGGTACTGTATGAACTGACAGATTGTGGAATTTTTCTTAAGGGTAACACCATTGCTGTGATTGGTGGTTCACTCCAAGGAATAAATATAGTACGGAGGATTGTGGAAGACTGCATTGCTCATGGTGTGCCTCCTCTACCCCGGGTGCAATTGATGAAAAAGAAGACTGAACAGAAGAAGGATGGGATCATCAAAGTGACGAGTGAACTGATGATGAATCTCCAGGCTATGCGGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

25.38

Weight (kDa)

5.15

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 28 - 105 1.7e-14 Krr1 KH1 domain
KH_KRR1_2nd PF21800 110 - 200 9.5e-12 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08420
fragaria_vesca FvH4_1g11160 FvH4_2g08143 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36460 FvH4_3g36460 FvH4_3g45301 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_5g09160 FvH4_5g09161 FvH4_5g09380 FvH4_5g22900 FvH4_6g42520 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550
malus_domestica MD02G1015700.v1.1 MD02G1058900.v1.1 MD02G1059000.v1.1 MD03G1170900.v1.1 MD04G1231500.v1.1 MD13G1044300.v1.1 MD16G1045100.v1.1
prunus_persica Prupe.1G308200_v2.0.a1 Prupe.2G125300_v2.0.a1 Prupe.6G307800_v2.0.a1 Prupe.6G351700_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1
pyrus_communis pycom02g01420 pycom02g04810 pycom03g12790 pycom03g12800 pycom12g17840 pycom12g17850 pycom12g17860 pycom13g03890 pycom16g03970
rosa_chinensis RchiOBHm_Chr2g0090081 RchiOBHm_Chr3g0469061 RchiOBHm_Chr3g0469081 RchiOBHm_Chr4g0435531 RchiOBHm_Chr5g0014021 RchiOBHm_Chr5g0014031 RchiOBHm_Chr5g0014041 RchiOBHm_Chr7g0190151 RchiOBHm_Chr7g0190161 RchiOBHm_Chr7g0190171 RchiOBHm_Chr7g0210441
rosa_laevigata RLG00000003683 RLG00000003687 RLG00000003688 RLG00000004561 RLG00000004643 RLG00000016083 RLG00000021085 RLG00000024381 RLG00000032065 RLG00000032067 RLG00000032071 RLG00000032072 RLG00000032075
rosa_multiflora Rmu_co8284349.1_g000001 Rmu_co8324735.1_g000001 Rmu_co8355915.1_g000001 Rmu_co8388905.1_g000001 Rmu_sc0000302.1_g000041 Rmu_sc0000302.1_g000050 Rmu_sc0001940.1_g000034 Rmu_sc0003807.1_g000011 Rmu_sc0005803.1_g000001 Rmu_sc0005803.1_g000007 Rmu_sc0007139.1_g000007 Rmu_sc0007767.1_g000001 Rmu_sc0007767.1_g000003 Rmu_sc0007767.1_g000006 Rmu_sc0007933.1_g000005 Rmu_sc0008587.1_g000004 Rmu_sc0008968.1_g000004 Rmu_sc0018491.1_g000002 Rmu_sc0022420.1_g000001 Rmu_sc0027700.1_g000001 Rmu_sc0036218.1_g000001
rosa_roxburghii Rroxscaffold_1G00062590 Rroxscaffold_1G00062600 Rroxscaffold_1G00062620 Rroxscaffold_1G00062630 Rroxscaffold_1G00062640 Rroxscaffold_1G00062670 Rroxscaffold_1G00062680 Rroxscaffold_2G00091430 Rroxscaffold_2G00151410 Rroxscaffold_3G00245340 Rroxscaffold_3G00255390 Rroxscaffold_3G00264820 Rroxscaffold_3G00264830 Rroxscaffold_3G00265870 Rroxscaffold_5G00355140 Rroxscaffold_5G00376760 Rroxscaffold_6G00412230
rosa_rugosa Rorug02G0005200 Rorug02G0468300 Rorug02G0468300 Rorug02G0532400 Rorug03G0098900 Rorug03G0099000 Rorug03G0099400 Rorug04G0286000 Rorug05G0014400 Rorug05G0014500 Rorug05G0014600 Rorug05G0014600 Rorug05G0014600 Rorug05G0014800 Rorug05G0014900 Rorug05G0015000 Rorug05G0015100 Rorug05G0015100 Rorug05G0441300 Rorug06G0495300 Rorug06G0502600 Rorug06G0502700 Rorug07G0062300
rosa_samantha Rh2AG050900 Rh2BG049600 Rh2CG051800 Rh2CG234600 Rh2DG051100 Rh3BG171300 Rh3BG171800 Rh3CG336700 Rh3DG203600 Rh3DG203800 Rh4AG341600 Rh4AG341700 Rh4BG349900 Rh4CG364600 Rh4DG344200 Rh5AG108800 Rh5AG108900 Rh5AG109000 Rh5AG109100 Rh5BG105500 Rh5CG117300 Rh5CG117500 Rh5CG117600 Rh5DG104200 Rh5DG104400 Rh5DG104500 Rh5DG104600 Rh7BG102300 Rh7BG110600 Rh7BG110700 Rh7BG118900 Rh7CG103600 Rh7CG112800 Rh7CG113000 Rh7CG200300 Rh7CG271100 Rh7DG111300 Rh7DG111400 Rh7DG194400 Rh7DG261800 Rh7DG261900
rosa_wichuraiana Rw0G014550 Rw0G014580 Rw2G004580 Rw3G013980 Rw3G013990 Rw3G014000 Rw3G014020 Rw4G029810 Rw5G009500 Rw5G009510 Rw5G009520 Rw5G009530 Rw7G008640 Rw7G009350 Rw7G021680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 23, 667
AclWI GGATC 3 cut(s) 231, 389, 629
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 2 cut(s) 98, 465
AfaI GTAC 5 cut(s) 309, 329, 377, 441, 528
AfiI CCNNNNNNNGG 3 cut(s) 22, 34, 184
AflII CTTAAG 1 cut(s) 473
AjnI CCWGG 2 cut(s) 177, 657
AluBI AGCT 2 cut(s) 268, 415
AluI AGCT 2 cut(s) 268, 415
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 3 cut(s) 231, 389, 629
Ama87I CYCGRG 1 cut(s) 576
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 2 cut(s) 98, 465
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 2 cut(s) 577, 578
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 133
BbsI GAAGAC 2 cut(s) 549, 605
BbvI GCAGC 1 cut(s) 105
BccI CCATC 1 cut(s) 611
BciT130I CCWGG 2 cut(s) 179, 659
BcnI CCSGG 2 cut(s) 577, 578
BcoDI GTCTC 1 cut(s) 79
BfrI CTTAAG 1 cut(s) 473
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 4 cut(s) 179, 577, 578, 659
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 576
BmgT120I GGNCC 1 cut(s) 25
BmrFI CCNGG 4 cut(s) 179, 577, 578, 659
BpiI GAAGAC 2 cut(s) 549, 605
BpmI CTGGAG 1 cut(s) 641
BpuMI CCSGG 2 cut(s) 577, 578
BsaJI CCNNGG 4 cut(s) 177, 511, 575, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 34, 184
Bse1I ACTGG 2 cut(s) 315, 325
Bse3DI GCAATG 2 cut(s) 486, 549
BseBI CCWGG 2 cut(s) 179, 659
BseDI CCNNGG 4 cut(s) 177, 511, 575, 576
BseGI GGATG 3 cut(s) 187, 262, 622
BseLI CCNNNNNNNGG 3 cut(s) 22, 34, 184
BseMI GCAATG 2 cut(s) 486, 549
BseMII CTCAG 2 cut(s) 39, 259
BseNI ACTGG 2 cut(s) 315, 325
BseRI GAGGAG 1 cut(s) 558
BseXI GCAGC 1 cut(s) 105
BshFI GGCC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 576
BsiSI CCGG 2 cut(s) 287, 577
BslI CCNNNNNNNGG 3 cut(s) 22, 34, 184
BsmAI GTCTC 1 cut(s) 79
BsmBI CGTCTC 1 cut(s) 79
BsnI GGCC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 576
Bsp143I GATC 4 cut(s) 30, 223, 381, 621
BspACI CCGC 2 cut(s) 23, 667
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 40, 258
BspPI GGATC 3 cut(s) 231, 389, 629
BspTI CTTAAG 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 486, 549
BsrI ACTGG 2 cut(s) 315, 325
BssECI CCNNGG 4 cut(s) 177, 511, 575, 576
BssMI GATC 4 cut(s) 30, 223, 381, 621
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 179, 659
Bst4CI ACNGT 1 cut(s) 444
Bst6I CTCTTC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 3 cut(s) 48, 245, 387
BstF5I GGATG 3 cut(s) 187, 262, 622
BstKTI GATC 4 cut(s) 33, 226, 384, 624
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 4 cut(s) 30, 223, 381, 621
BstMWI GCNNNNNNNGC 2 cut(s) 171, 296
BstNI CCWGG 2 cut(s) 179, 659
BstSCI CCNGG 4 cut(s) 177, 575, 576, 657
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 2 cut(s) 549, 605
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 3 cut(s) 187, 262, 622
Cac8I GCNNGC 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 25
Cfr9I CCCGGG 1 cut(s) 576
Csp6I GTAC 5 cut(s) 308, 328, 376, 440, 527
CviAII CATG 1 cut(s) 557
CviJI RGCY 8 cut(s) 133, 215, 251, 268, 415, 422, 426, 662
CviKI_1 RGCY 8 cut(s) 133, 215, 251, 268, 415, 422, 426, 662
CviQI GTAC 5 cut(s) 308, 328, 376, 440, 527
DdeI CTNAG 3 cut(s) 48, 245, 387
DpnI GATC 4 cut(s) 32, 225, 383, 623
DpnII GATC 4 cut(s) 30, 223, 381, 621
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 25
Eco88I CYCGRG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 2 cut(s) 177, 657
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 1 cut(s) 560
FaiI YATR 8 cut(s) 172, 254, 374, 429, 447, 524, 558, 665
FatI CATG 1 cut(s) 556
FauI CCCGC 1 cut(s) 660
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 3 cut(s) 194, 269, 629
Fsp4HI GCNGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 1 cut(s) 133
HapII CCGG 2 cut(s) 287, 577
Hin1II CATG 1 cut(s) 560
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 3 cut(s) 143, 349, 652
HpaII CCGG 2 cut(s) 287, 577
Hpy166II GTNNAC 2 cut(s) 506, 641
Hpy188I TCNGA 2 cut(s) 49, 674
Hpy188III TCNNGA 6 cut(s) 55, 103, 227, 276, 340, 353
Hpy8I GTNNAC 2 cut(s) 506, 641
HpyAV CCTTC 1 cut(s) 607
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4V TGCA 8 cut(s) 121, 174, 209, 318, 369, 404, 549, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 296
HpyF3I CTNAG 3 cut(s) 48, 245, 387
Hsp92II CATG 1 cut(s) 560
Kzo9I GATC 4 cut(s) 30, 223, 381, 621
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 479, 631
MalI GATC 4 cut(s) 32, 225, 383, 623
MboI GATC 4 cut(s) 30, 223, 381, 621
MboII GAAGA 4 cut(s) 368, 554, 610, 622
MfeI CAATTG 1 cut(s) 584
MlsI TGGCCA 1 cut(s) 133
MluCI AATT 4 cut(s) 98, 395, 465, 584
MluNI TGGCCA 1 cut(s) 133
MnlI CCTC 7 cut(s) 28, 184, 352, 402, 525, 576, 579
Mox20I TGGCCA 1 cut(s) 133
MscI TGGCCA 1 cut(s) 133
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 2 cut(s) 491, 629
Msp20I TGGCCA 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 2 cut(s) 287, 577
MspR9I CCNGG 4 cut(s) 179, 577, 578, 659
MunI CAATTG 1 cut(s) 584
MvaI CCWGG 2 cut(s) 179, 659
MwoI GCNNNNNNNGC 2 cut(s) 171, 296
NciI CCSGG 2 cut(s) 577, 578
NdeII GATC 4 cut(s) 30, 223, 381, 621
NlaIII CATG 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 631
PfeI GAWTC 3 cut(s) 143, 349, 652
PkrI GCNGC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 177, 657
PspGI CCWGG 2 cut(s) 177, 657
PspPI GGNCC 1 cut(s) 25
RsaI GTAC 5 cut(s) 309, 329, 377, 441, 528
RsaNI GTAC 5 cut(s) 308, 328, 376, 440, 527
RseI CAYNNNNRTG 2 cut(s) 491, 629
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 4 cut(s) 30, 223, 381, 621
Sau96I GGNCC 1 cut(s) 25
ScaI AGTACT 1 cut(s) 309
ScrFI CCNGG 4 cut(s) 179, 577, 578, 659
SetI ASST 4 cut(s) 39, 270, 417, 441
SinI GGWCC 1 cut(s) 25
SmaI CCCGGG 1 cut(s) 578
SmiMI CAYNNNNRTG 2 cut(s) 491, 629
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 4 cut(s) 98, 395, 465, 584
SsiI CCGC 2 cut(s) 23, 667
SspI AATATT 1 cut(s) 238
StyD4I CCNGG 4 cut(s) 177, 575, 576, 657
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 33, 54
TasI AATT 4 cut(s) 98, 395, 465, 584
TatI WGTACW 1 cut(s) 307
TfiI GAWTC 3 cut(s) 143, 349, 652
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 4 cut(s) 215, 462, 605, 665
TspGWI ACGGA 2 cut(s) 421, 544
TspMI CCCGGG 1 cut(s) 576
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 98, 465
XmaI CCCGGG 1 cut(s) 576
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.