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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 3 cut(s) | 77, 83, 152 |
| AoxI | GGCC | 2 cut(s) | 121, 184 |
| AspS9I | GGNCC | 1 cut(s) | 122 |
| BmgT120I | GGNCC | 1 cut(s) | 122 |
| BmsI | GCATC | 1 cut(s) | 39 |
| BshFI | GGCC | 2 cut(s) | 123, 186 |
| BsnI | GGCC | 2 cut(s) | 123, 186 |
| BspANI | GGCC | 2 cut(s) | 123, 186 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 146 |
| BstNSI | RCATGY | 1 cut(s) | 148 |
| BsuRI | GGCC | 2 cut(s) | 123, 186 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 146 |
| Cfr13I | GGNCC | 1 cut(s) | 122 |
| CseI | GACGC | 1 cut(s) | 163 |
| CviAII | CATG | 2 cut(s) | 145, 171 |
| CviJI | RGCY | 2 cut(s) | 123, 186 |
| CviKI_1 | RGCY | 2 cut(s) | 123, 186 |
| FaeI | CATG | 2 cut(s) | 148, 174 |
| FaiI | YATR | 6 cut(s) | 20, 66, 139, 141, 146, 172 |
| FatI | CATG | 2 cut(s) | 144, 170 |
| HaeIII | GGCC | 2 cut(s) | 123, 186 |
| HgaI | GACGC | 1 cut(s) | 163 |
| Hin1II | CATG | 2 cut(s) | 148, 174 |
| HincII | GTYRAC | 1 cut(s) | 30 |
| HindII | GTYRAC | 1 cut(s) | 30 |
| HinfI | GANTC | 2 cut(s) | 4, 38 |
| Hpy166II | GTNNAC | 1 cut(s) | 30 |
| Hpy188III | TCNNGA | 1 cut(s) | 8 |
| Hpy8I | GTNNAC | 1 cut(s) | 30 |
| Hpy99I | CGWCG | 1 cut(s) | 179 |
| HpyCH4V | TGCA | 1 cut(s) | 56 |
| Hsp92II | CATG | 2 cut(s) | 148, 174 |
| LpnPI | CCDG | 1 cut(s) | 21 |
| LweI | GCATC | 1 cut(s) | 39 |
| MaeIII | GTNAC | 2 cut(s) | 154, 190 |
| MboII | GAAGA | 1 cut(s) | 159 |
| MnlI | CCTC | 2 cut(s) | 15, 169 |
| MslI | CAYNNNNRTG | 1 cut(s) | 107 |
| NlaIII | CATG | 2 cut(s) | 148, 174 |
| NspI | RCATGY | 1 cut(s) | 148 |
| PaeI | GCATGC | 1 cut(s) | 148 |
| PfeI | GAWTC | 2 cut(s) | 4, 38 |
| PspPI | GGNCC | 1 cut(s) | 122 |
| RseI | CAYNNNNRTG | 1 cut(s) | 107 |
| Sau96I | GGNCC | 1 cut(s) | 122 |
| SetI | ASST | 2 cut(s) | 161, 197 |
| SfaNI | GCATC | 1 cut(s) | 39 |
| SgeI | CNNG | 7 cut(s) | 20, 27, 65, 157, 161, 183, 200 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 107 |
| SphI | GCATGC | 1 cut(s) | 148 |
| TaqI | TCGA | 1 cut(s) | 96 |
| TfiI | GAWTC | 2 cut(s) | 4, 38 |
| TspDTI | ATGAA | 2 cut(s) | 17, 159 |
| XceI | RCATGY | 1 cut(s) | 148 |